CN203947102U - A kind of device for nucleic acid rapid extraction - Google Patents
A kind of device for nucleic acid rapid extraction Download PDFInfo
- Publication number
- CN203947102U CN203947102U CN201420386073.4U CN201420386073U CN203947102U CN 203947102 U CN203947102 U CN 203947102U CN 201420386073 U CN201420386073 U CN 201420386073U CN 203947102 U CN203947102 U CN 203947102U
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- Prior art keywords
- adsorption film
- nucleic acid
- syringe
- rapid extraction
- interconnecting device
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- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 53
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 53
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 53
- 238000000605 extraction Methods 0.000 title claims abstract description 32
- 238000001179 sorption measurement Methods 0.000 claims abstract description 52
- 239000007788 liquid Substances 0.000 claims description 18
- 208000004141 microcephaly Diseases 0.000 claims description 5
- 238000001514 detection method Methods 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of device for nucleic acid rapid extraction of the utility model, comprise syringe, adsorption film and sample needle, also comprise the interconnecting device of a hollow, described adsorption film is arranged on the bottom in an adsorption film pipe, and interconnecting device is connected in the middle of syringe and adsorption film pipe and syringe, interconnecting device and adsorption film Guan Ye road are communicated with.The utility model is simple to operate, the operating time is short, approximately only need 10 within 15 minutes, can complete rapid extraction nucleic acid from various clinical samples (comprising RNA and DNA).
Description
Technical field
The utility model relate to a kind of can be from various clinical samples the device of rapid extraction nucleic acid.
Background technology
Nucleic acid extraction has become most important, the most basic operation in molecular biology experiment technology, but the extraction scheme of nucleic acid is many at present, and complicated and simple differing.The successful extraction that completes whole dependence nucleic acid of the related experiment such as molecular diagnosis, nucleic acid sequencing, functional genome, oneself can realize whole automatizations by warp traditional operating process, but these expensive instruments can not be promoted the use of on a large scale, China is still developing country at present, vast different medical unit can not be equipped with complicated instrument, therefore, provide a kind of nucleic acid-extracting apparatus of good economy performance necessary.
CN101684463A proposes a kind of trace clinical sample nucleic acid rapid extraction device, by sharp-crested suction pipe, strainer with film and suction unit (syringe or plastic blister) three part compositions, it has aspirated nucleic acid absorption by three times, clean and elution process, the fast and convenient nucleic acid that extracts from sample of energy, so blemish in an otherwise perfect thing is, sharp-crested suction pipe, strainer with film and suction unit three part array modes single and for fixed installation, (Fig. 2 of CN101684463A while making suction unit by plastic blister, Fig. 3) suction for the third time carries out that nucleic acid wash-out is actual can not complete that (waste liquid of front twice suction cannot be discharged, elutriant with nucleic acid is mixed in waste liquid), and use syringe while making suction unit, enter syringe with the elutriant of nucleic acid and not only can be infected with syringe formation biologic garbage, also can cause trace sample amplifying nucleic acid be injected device be infected with and lose cause detect inaccurate.
Utility model content
For overcoming problems of the prior art, the utility model object be to provide a kind of simple, practical, efficient for the device of nucleic acid rapid extraction.
Device for nucleic acid rapid extraction of the present utility model, comprise syringe, adsorption film and sample needle, also comprise the interconnecting device of a hollow, described adsorption film is arranged on the bottom in an adsorption film pipe, and interconnecting device is connected in the middle of syringe and adsorption film pipe and syringe, interconnecting device and adsorption film Guan Ye road are communicated with.
Wherein, the body that adsorption film pipe is both ends open, the opening end that one end is this body, the other end is fixed an osculum pipe connecting, and adsorption film is arranged on this osculum pipe connecting one end.
Adsorption film pipe opening end size is mated with the outside dimension of interconnecting device.
Syringe comprises cylindrical shell, be placed in the tubulose Connection Block of barrel front end and be placed in cylindrical shell can push-and-pull push rod, the external diameter of described osculum pipe connecting equates with the Connection Block outside dimension of syringe, described interconnecting device is established intermediate throughholes, and intermediate throughholes size is mated with the Connection Block outside dimension of syringe.
Described sample needle comprises needle stand and syringe needle, and the external diameter of described osculum pipe connecting mates with the needle stand internal diameter of described sample needle.
Interconnecting device one end is communicated with syringe, and the other end is communicated with the osculum pipe connecting of adsorption film pipe, forms the drawing liquid mode of this device.Or interconnecting device one end is communicated with syringe, the other end is socketed in the opening end of adsorption film pipe, and the osculum pipe connecting of adsorption film pipe is communicated with sample needle, forms the liquid mode that pushes away of this device.
Described interconnecting device profile is round table-like, and the microcephaly of round table-like interconnecting device holds outside dimension to mate with the opening end of adsorption film pipe, and this opening end is held socket from the microcephaly of round table-like interconnecting device.
The utility model adopts bulk-breaking mode, by the assembling use of arranging in pairs or groups, is skillfully constructed, simple in structure, practical.Wherein, interconnecting device two ends can connect different parts simultaneously, and adsorption film pipe detachably and oppositely assembles, and are used in conjunction with hollow and the identical interconnecting device of size, complete the collocation of nucleic acid extraction from absorption washing to wash-out different steps, make nucleic acid extraction simple, quick, efficient.
The utility model can be used as the front simple and direct means of one of biological specimen being carried out to nucleic acid extraction of nucleic acid amplification, without any need for instrument, operational safety, simple, disposable use, thereby can be widely used in pathogenic agent or host's nucleic acid extraction, for clinical detection and diagnosis.The utility model extraction element is with low cost, while simple to operate is again without any need for specific apparatus, operating time is short, approximately only need 10 ?15 minutes, thereby the utility model can be widely used in the relatively poor basic hospital of some experiment conditions and economically less developed region or country; Meanwhile, also can meet well the demand, particularly emergency room of high-level hospital and the nucleic acid rapid detection of bedside.
Brief description of the drawings
Fig. 1 is the device exploded view of the utility model for nucleic acid rapid extraction;
Decomposition and constitutional diagram when Fig. 2 device is exercised pumping function;
Decomposition and constitutional diagram when Fig. 3 device is exercised pushing function.
Embodiment
The utility model, for the device of nucleic acid rapid extraction, is mainly used in rapid extraction nucleic acid from various clinical samples (comprising RNA and DNA).
Referring to figure 1 ?shown in Fig. 3, this device mainly comprises syringe 1, adsorption film pipe 2, interconnecting device 3 and 4 four parts of sample needle, wherein:
Syringe 1 is common tubular disposable syringe or the allied equipment that can exercise syringe function, it has cylindrical shell 11, be placed in cylindrical shell 11 front ends for connect the tubulose Connection Block 12 of syringe needle and be placed in cylindrical shell 11 can push-and-pull push rod 13; Sample needle 4 is common injection needles, comprises needle stand 41 and syringe needle 42.
Cylinder or round platform that interconnecting device 3 is hollow, its intermediate throughholes 31 sizes are mated with syringe 1 tubulose Connection Block 12 outside dimensions, and this tubulose Connection Block 12 can be inserted in interconnecting device 3 intermediate throughholes 31; Separately, interconnecting device 3 appearances are cylindric or round table-like, and its outside dimension mates with adsorption film pipe 2, below describe in detail; Interconnecting device 3 can have elastic material and make as rubber, plastics.
The body that adsorption film pipe 2 is both ends open, available plastic material is made, its one end is the opening end 21 of this body, the other end is fixed an osculum pipe connecting 22, adsorption film pipe 2 inner bottom parts (pipe connecting 22 one end are called the end) are established adsorption film 23 (commercial goods, as pellosil Silica membrane); The both ends open of adsorption film pipe 2 all can be connected with interconnecting device 3, and wherein opening end 21 sizes are mated with the outside dimension of cylindric interconnecting device 3, or holds outside dimension to mate with the microcephaly of round table-like interconnecting device so that interconnecting device 3 can be socketed with opening end 21; Osculum pipe connecting 22 external diameters of adsorption film pipe 2 are identical with syringe 1 Connection Block 12, both mated with needle stand 41 internal diameters of sample needle 4, again with interconnecting device 3 intermediate throughholes 31 size match, make this osculum pipe connecting 22 can be inserted into the interior connection sample needle 4 of needle stand 41, can be inserted into again connection adapter 3 in intermediate throughholes 31.
In the utility model, while extracting solution, connect the Connection Block 12 of syringe 1 with interconnecting device 3 one end, the other end connects the osculum pipe connecting 22 of adsorption film pipe 2, make adsorption film 23 rear (in the utility model with syringe user to definition before and after, immediate shipment syringe needle one end is defined as " front ", and when push rod extracts, direction of motion is defined as " afterwards "), combine the drawing liquid form (as shown in Figure 2) of nucleic acid rapid extraction device.While releasing solution, connect the Connection Block 12 of syringe 1 with interconnecting device 3 one end, the other end stretches into the opening end 21 of adsorption film pipe 2, and the osculum pipe connecting 22 of adsorption film pipe 2 connects the needle stand 41 of sample needle 4 again, combines the liquid form that pushes away (as shown in Figure 3) of nucleic acid rapid extraction device.
While utilizing the utility model to extract nucleic acid, operate like this:
1, use drawing liquid form, aspirate with the opening end 21 of adsorption film pipe 2 from sample lysate, lysate enters in cylindrical shell 11 by adsorption film 23, and the nucleic acid discharging after sample cracking is adsorbed on adsorption film 23;
2, use drawing liquid form, aspirate rinsing liquid with the opening end 21 of adsorption film pipe 2, rinsing liquid enters in cylindrical shell 11 by adsorption film 23, so just can be by subsidiary organic solvent, the cell debris of nucleic acid being adsorbed on film by the cleaning function of rinsing liquid, the foreign material such as protein are brought in cylindrical shell 11, and the waste liquid entering in cylindrical shell 11 is processed in addition;
3, change the syringe 1 of another cleaning, wherein first suck elutriant, then use and push away the assembling of liquid form: by reverse adsorption film pipe 2, make adsorption film 23 in front (as shown in Figure 3), the opening end 21 of adsorption film pipe 2 is socketed interconnecting device 3 one end, and the osculum pipe connecting 22 of adsorption film pipe 2 connects the needle stand 41 of sample needle 4; The push rod 13 of pushing syringe 1 forward, the elutriant in cylindrical shell 11, by the nucleic acid wash-out on adsorption film 23, is extruded into elutriant and nucleic acid other nucleic acid collection container from sample needle 4 by the strength of pushing, completes the leaching process of sample amplifying nucleic acid.
Use the utility model to have the advantage of nucleic acid extraction to be, each parts match is coincide, and uses the flexible assembly of each parts, with drawing liquid form and the extraction and the release that push away liquid form and complete different solutions, easy to use respectively; When wash-out is adsorbed on the nucleic acid on film, uses and push away liquid mode, can directly nucleic acid be pushed in nucleic acid collection container, both prevented pollution of nucleic acid entry needle, can not make nucleic acid samples loss because barrel wall adheres to nucleic acid two again; In addition, adsorption film pipe front end connects the point sample that sample needle contributes to follow-up detection of nucleic acids, reduces transitional link to ensure the continuity of nucleic acid extraction and subsequent detection equally as far as possible.
Claims (8)
1. for the device of nucleic acid rapid extraction, comprise syringe, adsorption film and sample needle, it is characterized in that, also comprise the interconnecting device of a hollow, described adsorption film is arranged on the bottom in an adsorption film pipe, and interconnecting device is connected in the middle of syringe and adsorption film pipe and syringe, interconnecting device and adsorption film Guan Ye road are communicated with.
2. according to claim 1 for the device of nucleic acid rapid extraction, it is characterized in that, the body that adsorption film pipe is both ends open, the opening end that one end is this body, the other end is fixed an osculum pipe connecting, and adsorption film is arranged on this osculum pipe connecting one end.
3. according to claim 2 for the device of nucleic acid rapid extraction, it is characterized in that, adsorption film pipe opening end size is mated with the outside dimension of interconnecting device.
4. according to claim 3 for the device of nucleic acid rapid extraction, it is characterized in that, syringe comprises cylindrical shell, be placed in the tubulose Connection Block of barrel front end and be placed in cylindrical shell can push-and-pull push rod, the external diameter of described osculum pipe connecting equates with the Connection Block outside dimension of syringe, described interconnecting device is established intermediate throughholes, and intermediate throughholes size is mated with the Connection Block outside dimension of syringe.
5. according to claim 3 for the device of nucleic acid rapid extraction, it is characterized in that, described sample needle comprises needle stand and syringe needle, and the external diameter of described osculum pipe connecting mates with the needle stand internal diameter of described sample needle.
6. according to the device for nucleic acid rapid extraction described in claim 2 or 3 or 4, it is characterized in that, interconnecting device one end is communicated with syringe, and the other end is communicated with the osculum pipe connecting of adsorption film pipe, forms the drawing liquid mode of this device.
7. according to the device for nucleic acid rapid extraction described in claim 2 or 3 or 4 or 5, it is characterized in that, interconnecting device one end is communicated with syringe, and the other end is socketed in the opening end of adsorption film pipe, the osculum pipe connecting of adsorption film pipe is communicated with sample needle, forms the liquid mode that pushes away of this device.
8. according to claim 7 for the device of nucleic acid rapid extraction, it is characterized in that, described interconnecting device profile is round table-like, and the microcephaly of round table-like interconnecting device holds outside dimension to mate with the opening end of adsorption film pipe, and this opening end is held socket from the microcephaly of round table-like interconnecting device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420386073.4U CN203947102U (en) | 2013-07-12 | 2014-07-14 | A kind of device for nucleic acid rapid extraction |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310292120.9 | 2013-07-12 | ||
| CN201310292120 | 2013-07-12 | ||
| CN201420386073.4U CN203947102U (en) | 2013-07-12 | 2014-07-14 | A kind of device for nucleic acid rapid extraction |
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| Publication Number | Publication Date |
|---|---|
| CN203947102U true CN203947102U (en) | 2014-11-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420386073.4U Expired - Lifetime CN203947102U (en) | 2013-07-12 | 2014-07-14 | A kind of device for nucleic acid rapid extraction |
| CN201410333236.7A Active CN104059849B (en) | 2013-07-12 | 2014-07-14 | Device and method used for rapid extraction of nucleic acid |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410333236.7A Active CN104059849B (en) | 2013-07-12 | 2014-07-14 | Device and method used for rapid extraction of nucleic acid |
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| CN (2) | CN203947102U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104059849A (en) * | 2013-07-12 | 2014-09-24 | 中国人民解放军疾病预防控制所 | Device and method used for rapid extraction of nucleic acid |
| WO2019001228A1 (en) * | 2017-06-30 | 2019-01-03 | 开启基因股份有限公司 | Fixed tube of nucleic acid extraction component, and nucleic acid extraction component |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105567670A (en) * | 2014-10-11 | 2016-05-11 | 中国医学科学院基础医学研究所 | Method and apparatus for preserving nucleic acid in urine sample |
| CN104593252B (en) * | 2015-01-12 | 2017-02-22 | 国家纳米科学中心 | Sample pretreatment and amplification integrated nucleic acid analysis system based on pipette tip and application of system |
| CN105779277A (en) * | 2015-01-13 | 2016-07-20 | 温和心 | Nucleic acid extraction suction tube |
| JP2016158557A (en) * | 2015-03-02 | 2016-09-05 | セイコーエプソン株式会社 | Container, biological substance purification cartridge, and assembly kit for biological substance purification cartridge |
| CN105296327B (en) * | 2015-10-21 | 2017-10-27 | 陈辉 | A kind of nucleic acid-extracting apparatus and its extracting method |
| CN106047660A (en) * | 2016-07-07 | 2016-10-26 | 李丽倩 | Nucleic acid extraction device for nucleic acid isolation machine |
| CN106591107B (en) * | 2017-01-12 | 2019-04-12 | 武汉菲思特生物科技有限公司 | Sample adding device for pyrosequencing |
| CN106947683B (en) * | 2017-05-24 | 2024-02-20 | 苏州天隆生物科技有限公司 | Nucleic acid extraction and purification device and method |
| CN107574165A (en) * | 2017-09-27 | 2018-01-12 | 广州和实生物技术有限公司 | A kind of method for extracting nucleic acid and the extraction element for realizing this method |
| CN110272807B (en) * | 2018-03-13 | 2020-11-24 | 武汉医蒂生物科技有限公司 | Positive pressure device for nucleic acid extraction and purification |
| CN110437980B (en) * | 2019-08-28 | 2023-12-22 | 湖北微伞医疗科技有限公司 | Nucleic acid extraction adsorption column |
| CN113930318B (en) * | 2020-07-13 | 2024-11-12 | 山东凯景生物技术有限公司 | Nucleic acid extraction consumables and method for extracting nucleic acid using the same |
| CN113249188A (en) * | 2021-06-03 | 2021-08-13 | 宁波康程德诺生物医药有限公司 | Rapid nucleic acid extraction device and method for extracting nucleic acid |
| CN114045207B (en) * | 2021-09-26 | 2024-02-06 | 厦门健康工程与创新研究院 | Nucleic acid extraction detection system and method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4102149B2 (en) * | 2002-09-25 | 2008-06-18 | 富士フイルム株式会社 | Nucleic acid separation and purification equipment |
| CN101684463A (en) * | 2008-09-28 | 2010-03-31 | 杭州优思达生物技术有限公司 | Method and kit for rapidly extracting nucleic acids from trace clinical samples |
| CN102409079A (en) * | 2010-08-26 | 2012-04-11 | 杭州优思达生物技术有限公司 | Novel infectious disease nucleic acid rapid detection kit and detection method thereof |
| CN203947102U (en) * | 2013-07-12 | 2014-11-19 | 中国人民解放军疾病预防控制所 | A kind of device for nucleic acid rapid extraction |
-
2014
- 2014-07-14 CN CN201420386073.4U patent/CN203947102U/en not_active Expired - Lifetime
- 2014-07-14 CN CN201410333236.7A patent/CN104059849B/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104059849A (en) * | 2013-07-12 | 2014-09-24 | 中国人民解放军疾病预防控制所 | Device and method used for rapid extraction of nucleic acid |
| WO2019001228A1 (en) * | 2017-06-30 | 2019-01-03 | 开启基因股份有限公司 | Fixed tube of nucleic acid extraction component, and nucleic acid extraction component |
| CN109207340A (en) * | 2017-06-30 | 2019-01-15 | 开启基因股份有限公司 | Fixing tube of nucleic acid extraction assembly and nucleic acid extraction assembly |
| US11365406B2 (en) * | 2017-06-30 | 2022-06-21 | Catchgene Co., Ltd. | Fixed tube of nucleic acid extraction component, and nucleic acid extraction component |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104059849B (en) | 2017-01-18 |
| CN104059849A (en) | 2014-09-24 |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |