CN203813679U - Split type electrophoresis power supply - Google Patents

Abstract

The utility model discloses a split type electrophoresis apparatus power supply. The electrophoresis apparatus power supply and the electrophoresis apparatus are independently designed. The power supply adopts a modulated design for its internal part, and it comprises a voltage reducing module which is mutually connected with a motherboard, a voltage raising module and an operation display module. The motherboard comprises a control module and a regulation module that are also connected with each other. The power supply of utility model is compatible with a traditional tubular type or horizontal type electric focusing groove and adopts the latest IPG-2-DE technology, which overcomes the inadaptability in prior art.

Description

Split type electrophoresis power supply

technical field

The utility model relates to a power supply for an electrophoresis instrument, in particular to a power supply for an electrophoresis instrument with special functions.

Background technique

Biological science research has entered the post-gene era, that is, the era of proteomics. Equipoint focusing is currently the most effective method of proteomics in the world. Traditional isoelectric focusing requires a voltage of less than 3000v and a current of less than 1 mA, while the latest isoelectric focusing technology IPG-2-DE (solid-phase pH gradient gel two-dimensional electrophoresis) requires 10000v or even higher and a current of tens of to several hundred microamperes. Therefore, isoelectric focusing requires a wide voltage range, usually in the ultra-high voltage range, and high current accuracy, generally in the microampere level.

At present, domestic bioelectrophoresis power supplies do not have electrophoresis power supplies with a voltage exceeding 5000v, and power supplies with currents displayed at the microampere level are even rarer.

Although foreign isoelectric focusing equipment meets the requirements of ultra-high voltage and microampere current, it is generally an integrated machine, which can neither meet the requirements of traditional isopoint focusing, nor can it be used in conjunction with domestic split electrophoresis devices.

Therefore, it is very necessary to develop a split-type electrophoresis power supply with ultra-high voltage (0-10000v) and high-precision current (accuracy up to 1uA).

Contents of the invention

The purpose of the utility model is to overcome the incompatibility of existing products, and provide a power supply for electrophoresis apparatus with very good adaptability and special voltage function and current function in market space.

In order to achieve the above object, the utility model adopts the following technical solutions:

The power supply of the electrophoresis instrument and the electrophoresis instrument adopt a separate independent design. The interior of the power supply adopts a modular design, including a step-down module, a boost module and a display operation module connected to the main board. The main board includes a control module and an adjustment module connected to each other. The module is mainly responsible for output voltage control and output current control, the adjustment module is mainly responsible for output voltage conversion acquisition and output current conversion acquisition, various modes and parameter storage and display are realized by the display operation module.

The step-down module converts AC220V into DC24V, and then the step-up module boosts the voltage to DC high voltage for output.

Compared with the prior art, the utility model has the following advantages and beneficial effects:

1. The advantage of the separate design of the power supply is that it is compatible with traditional tubular and horizontal isoelectric focusing tanks, and can also be equipped with the latest IPG-2-DE technology;

2. The output voltage of the power supply can meet the requirements of traditional isoelectric focusing with a voltage below 3000v, and can also meet the requirements of IPG-2-DE with a voltage of 10000v;

3. The current of the power supply can be controlled and displayed accurately to 1uA. This can ensure real-time monitoring of current changes in the early stage and during the electrophoresis experiment, creating conditions for the success and repeatability of biological experiments.

The utility model has many practical functions close to the needs of customers, mainly as follows:

The power program part can realize functions such as VH, fast and slow (linear) mode, and step by step. These functions are practical functions and operations for users, such as VH mode, this function can flexibly control the time of the experiment when the voltage is uncertain, so as to ensure that the experiment is completed accurately, and the step by step provides An automatic function is provided by which the user can simplify and store the conditions and status of experiments.

Description of drawings

Figure 1 Schematic diagram of the front panel;

Fig. 2 is the principle diagram of the mainboard of the power supply of the electrophoresis instrument of the present invention;

Fig. 3 is a working interface diagram of the power supply of the present invention;

Fig. 4 is the flow chart of the utility model power supply current program control.

The labels in the figure are as follows:

1. LCD display; 2. Value adjustment button (-); 3. Number adjustment button (+); 4. Selection button; 5. Confirmation button; 6. Electrode jack.

Detailed ways

Now describe the utility model in detail in conjunction with accompanying drawing:

The mechanical part of the split-type independent power supply is shown in Figure 1, which is composed of the electrode jack 6 and the corresponding connecting wires to complete the separate and independent operation of the power supply and the electrophoresis instrument. Among them: the liquid crystal display 1 displays the operation structure, and the corresponding commands are executed by adjusting the value adjustment button (-) 2, the number adjustment button (+) 3, the selection button 4 and the confirmation button 5.

The circuit part is shown in Figure 2, including a step-down module, a boost module and a display operation module connected to the main board. The main board includes a control module and an adjustment module connected to each other. The main working principle of the circuit is as follows:

As shown in the figure, the main circuit of the power supply first converts AC220V to DC24V by the step-down module, and then boosts the voltage to DC high voltage by the step-up module for output. When the voltage is boosted, it is controlled by the corresponding software and hardware on the main board. The control part is composed of a control module and an adjustment module. The control module is mainly responsible for output voltage control and output current control. The adjustment module is mainly responsible for output voltage conversion acquisition and output current conversion acquisition. Various modes and parameters are stored and displayed by the display operation module. .

The following describes its structural composition by function:

The composition and use of high-voltage functions are relatively simple. Its structure is composed of hardware circuits and machine control software. The hardware circuits are composed of voltage references, operational amplifiers, precision resistance-capacitance circuits, and memory. The software is mainly the control software of the machine, including control software, detection software, storage software and so on.

The composition of the high-precision current function is relatively complicated, and the use is relatively simple. Its structure is composed of hardware circuit and machine control software. The hardware circuit is composed of voltage reference, operational amplifier, precision resistance-capacitance circuit, and memory. In addition to the machine control software, the software mainly relies on acquisition and display software and pid algorithm. Control software to realize high-precision current display and control. When in use, it can automatically realize the display and control of microampere current according to the setting.

The utility model work process is as follows:

The use of power supply voltage and current must first set parameters and corresponding modes, etc., and then start the power supply to complete the output.

The working process of high-voltage output is relatively simple. The specific parameter setting process is related to the specific experiment and will not be described in detail here. After the parameter setting is completed and the power is turned on, the control software calls out the setting parameters and writes them into the control module. The hardware components of the control module are set according to the parameters Transform the voltage, and at the same time adjust the module to monitor and collect the output results in real time and send them back to the display module;

As shown in Figure 4, the working process of the high-precision current function is relatively complicated, and its parameter setting process is similar to that of the voltage. After the power is turned on, the control software calls out the setting parameters and writes them into the control module. The control completes the transformation of the electricity, and at the same time, the monitoring and collection results of the adjustment module include parameters such as voltage and current. The voltage parameter is sent back to the display operation module for display; after the current parameter is transformed, part of it is sent back to the control module through the pid algorithm to control the current, and part of it is sent back to the display operation module to display, and the two parts are completed in coordination to achieve high-precision current output control and display Function.

The current control part involves many time parameters: the main adjustment process should be completed in 1-2 minutes, the normal single-cycle delay should be within 20ms, and the adjustment cycle in the later stage of PID adjustment should be extended to 100-200ms to ensure no overshoot.

Claims (2)

1. a split type electrophoresis instrument power supply, electrophoresis apparatus power supply and electrophoresis apparatus adopt split type independent design, it is characterized in that: power supply inside comprises and the interconnective voltage reduction module of mainboard, boost module and display operation module, mainboard comprises interconnective control module and adjustment module, control module is mainly responsible for output voltage control and output current and is controlled, adjustment module mainly responsible output voltage conversion gathers and output current conversion collection, and various patterns and Parameter storage and demonstration are realized by display operation module.

2. split type electrophoresis instrument power supply according to claim 1, is characterized in that: described voltage reduction module becomes DC24V by AC220V, then by boost module, boosts to high direct voltage, exports.