CN203807475U - Quick bacteria detection device based on micro-fluidic chip - Google Patents
Quick bacteria detection device based on micro-fluidic chip Download PDFInfo
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- CN203807475U CN203807475U CN201320892495.4U CN201320892495U CN203807475U CN 203807475 U CN203807475 U CN 203807475U CN 201320892495 U CN201320892495 U CN 201320892495U CN 203807475 U CN203807475 U CN 203807475U
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Abstract
The utility model relates to the technical field of molecular organism monitoring, and discloses a quick bacteria detection device based on a micro-fluidic chip. The quick bacteria detection device comprises a bacteria detection device main body, wherein the bacteria detection device main body comprises a temperature control module, a fluorescent detection module, a power supply drive module, and a data processing and control module, wherein the temperature control module, the fluorescent detection module and the power supply drive module are all connected with the data processing and control module; a chip insertion opening is formed in the bacteria detection device main body; the micro-fluidic chip is mounted inside the chip insertion opening; the micro-fluidic chip is formed in the way that the upper layer of a base plate and the lower layer of the base plate are bonded and connected in a sealing manner; the lower layer of the base plate comprises a PCR reaction channel and a CE separation channel; grooves used as reagent tanks are formed in the end openings of the PCR reaction channel and the CE separation channel; the upper layer of the base plate comprises a hole used for filling a reagent and placing an electrode; the hole corresponds to the groove of the lower layer of the base plate in position. According to the quick bacteria detection device, the automation degree and the working efficiency of the analyzing process are improved.
Description
Technical field
The utility model relates to molecular biosciences monitoring technical field, is specifically related to bacteria detecting apparatus main body.
Background technology
PCR is the process that realizes in vitro DNA cloning, by the primer of polysaccharase and particular design, can from the DNA sequence dna of sample, copy the specific fragment of " significant ", and can make the quantity amplification of specific fragment reach 109 times.
Use capillary electrophoresis (Capillary Electrophoresis, CE) according to fragment size, to carry out separation to DNA fragmentation, detect the specific fragment with length-specific.CE is the electrophoresis carrying out in capillary channel, uses electric field driven DNA molecular, utilizes the interaction of sieving medium and DNA molecular simultaneously, makes the DNA fragmentation generation electrophoretic velocity of different lengths poor, reaches the object that detects DNA fragmentation length.CE is available higher separation voltage conventionally, and just can complete separation within a short period of time.
The operating process of conventional genetic analysis is more loaded down with trivial details, conventionally need to use as a series of equipment such as whizzer, temperature cycler, plate gel electrophoresis instrument, fluorescence imaging analyser or capillary electrophoresis apparatus, this has all proposed higher requirement to the hardware condition in laboratory and experimenter's operant level, is also difficult for reducing reagent and the cost of labor occurring in testing process.And sample shifts at a plurality of equipment rooms, increased the possibility of misoperation and sample contamination.
Along with the concept of micro-total analysis system (μ TAS) is suggested, the development that molecular biosciences detects has entered the new stage.Micro-total analysis system be take microflow control technique as basis, the miniaturization of analyzing and testing equipment, integrated and automatization have been promoted, not only can reduce acquisition cost, reagent cost and the manual operation cost of equipment, also for detecting real time implementation and the scene of monitoring, provide technological approaches.
Utility model content
The purpose of this utility model is to provide a kind of fast-bacteria-detection device based on micro-fluidic chip, to solve the problems of the technologies described above.
The technical problem that the utility model solves can realize by the following technical solutions:
A kind of fast-bacteria-detection device based on micro-fluidic chip, comprise a bacteria detecting apparatus main body, bacteria detecting apparatus main body comprises temperature control module, fluoroscopic examination module, electric power driving module, data processing and control module, described temperature control module, fluoroscopic examination module, electric power driving module all connect described data processing and control module, it is characterized in that, described bacteria detecting apparatus main body is provided with a chip socket, one micro-fluidic chip is installed in described chip socket, and described micro-fluidic chip is formed by top substrate layer and the sealing-in of lower laminar substrate bonding;
Described lower laminar substrate comprises a PCR reaction channel, a CE split tunnel, and the port of described PCR reaction channel and described CE split tunnel is equipped with the groove as reagent pond;
Described top substrate layer comprises that one for filling the hole of reagent and arrangement electrode, and the position in described hole is corresponding with the groove location of described lower laminar substrate.
The utility model is integrated in PCR and CE on micro-fluidic chip, is applicable to Bacteria Detection, has cancelled the manual operation link between PCR and CE, has improved level of automation and the working efficiency of analytic process.PCR coordinates CE to carry out the detection of cell, bacterium and virus, has high specificity, highly sensitive, fireballing advantage.Not only utilized the intrinsic advantage of PCR and CE, and further improved analysis efficiency, simplified the operation, improved the level of automation of proofing unit, for the rapid detection of bacterium, extensive examination and the quick diagnosis of disease provide technical support.
Temperature control module is for what heat with temperature control to PCR reaction channel, and described temperature control module is arranged on described micro-fluidic chip;
Described temperature control module comprises for changing the semiconductor temperature-control sheet of the temperature of PCR reaction channel, also comprises for measuring the temperature sensor of the temperature of PCR reaction channel; Described temperature control module is connected with control module with a data processing.
Temperature sensor is electrical signal by temperature transition.Electric signal transmission, to data processing and control module, is produced the feedback control signal of semiconductor temperature-control sheet by the electrical signal of temperature according to predefined algorithm, make temperature control sheet can keep PCR reaction channel in constant design temperature.
Described semiconductor temperature-control sheet is fitted in the one or two sides of described micro-fluidic chip.
When described semiconductor temperature-control sheet is fitted in the one side of described micro-fluidic chip, the quantity of described semiconductor temperature-control sheet is 2～3, and the orientation of temperature control sheet is vertical with the orientation of the straight line portion of PCR reaction channel.Each temperature control sheet is across all straight line portioies of PCR reaction channel, and each straight line portion of PCR reaction channel is heated as by all temperature control sheets several humidity provinces that temperature is different, for sex change, extension and the annealing of PCR reaction.
Fluoroscopic examination module is for gathering CE split tunnel fluorescent signal, and described fluoroscopic examination module connects described data processing and control module, and described data processing module connects and shows and interface module;
Described fluoroscopic examination module comprises excitation light source, optical delivery unit, photoelectric detector, and the range of exposures of described excitation light source covers described CE split tunnel.
Excitation light source produces the exciting light of narrower spectral bandwidth, and irradiates the detection window on CE split tunnel, excites the DNA fragmentation that contains fluorescence molecule to send fluorescence.Optical delivery unit is used for transmitting exciting light and collects fluorescence, and filtering wavelength equals to be less than the scattered light of exciting light.Photoelectric detector converts fluorescent signal to electrical signal, is transferred to data processing and control module.
Excitation light source can be the combination of photodiode, laser diode, broad spectrum light source and spike filter etc.
Optical delivery unit can be freeboard, optical waveguides (for example optical fiber) and lens.
Photoelectric detector can be photorectifier, phototriode, photomultiplier, charge coupled device etc.
Described electric power driving module comprises PCR driving power and CE driving power; PCR driving power is placed on respectively the initiating terminal of PCR reaction channel with two and the electrode of end is connected.Be used for driving DNA molecular and pcr amplification product Continuous Flow thereof to cross PCR reaction channel.
Described CE driving power is placed on respectively the initiating terminal of CE split tunnel with two and the electrode of end is connected.For driving the DNA fragmentation after amplification to flow through CE split tunnel.
Described data processing and control module realize the Control and coordination of temperature control module, fluoroscopic examination module, electric power driving module.The semiconductor temperature-control sheet of controlling temperature control module heats PCR reaction channel, from temperature sensor, accept temperature measurement signal simultaneously, produce feedback control signal, make temperature control module before reaching design temperature to PCR reaction channel continuous heating, after reaching design temperature, keep the homo(io)thermism of PCR reaction channel; Control the PCR driving power in electric power driving module, make DNA molecular and pcr amplification product Continuous Flow thereof cross PCR reaction channel; DNA fragmentation after pcr amplification has passed through the cross section of CE split tunnel, stops PCR driving power, opens CE driving power, makes the DNA fragmentation after amplification flow through CE split tunnel; Control the fluorescent signal that fluoroscopic examination module gathers CE split tunnel detection window place, and the signal obtaining is processed and stored.
Described demonstration and interface module are used for showing detection data, or with other equipment connections such as flash memory device, hard disc apparatus, computer, output test data.
Described top substrate layer material and described lower floor baseplate material can be the baseplate materials of any one material in glass, quartz, polydimethylsiloxane (Polydimethylsiloxane, PDMS).Thereby the material that makes top substrate layer and lower laminar substrate has certain physical strength and good optical transmittance
Described PCR reaction channel is a tortuous passage, comprise straight line portion and curved part, described straight line portion comprises a plurality of beeline channels of parallel arrangement, described curved part comprises a plurality of curved channels, the left end of a beeline channel is connected with the left end of adjacent next beeline channel by curved channel, and right-hand member passes through a curved channel and is connected with the right-hand member of an adjacent upper beeline channel;
The initiating terminal of described PCR reaction channel is provided with the groove as sample inlet pool; The end of described PCR reaction channel is provided with the groove as waste liquid pool.
Described CE split tunnel is straight line passage, or as described in the tortuous passageway of PCR reaction channel formula.Be that described CE split tunnel is several beeline channels that coupled together by bending channel.
The initiating terminal of described CE split tunnel is provided with the groove as separated liquid pool; The end of described CE split tunnel is provided with as waste liquid pool groove.
Last straight line portion at described PCR reaction channel end intersects with described CE split tunnel; Described intersection can be right-angled intersection, or double-T intersects.
While using described fast-bacteria-detection device, in the PCR of micro-fluidic chip reaction channel, fill PCR reaction solution, in CE split tunnel, fill parting liquid; Get the sample inlet pool that the reagent (for example 1 μ L) that contains in right amount sample DNA injects micro-fluidic chip; Micro-fluidic chip is inserted to proofing unit, the electrode of electric power driving module is placed in the slotted eye of micro-fluidic chip; Open this device, then this device will complete all subsequent steps that comprise PCR, CE, detection and data output automatically under the control in data processing and control module.First, data processing and control module opening temp. control module, make each temperature control sheet heating then keep design temperature, forms 2～3 different humidity provinces in PCR reaction channel; Then, the PCR driving power of power-on driver module, drives sample DNA and pcr amplification product Continuous Flow thereof to cross PCR reaction channel, completes PCR reaction, and the cross section of process CE split tunnel; Then, automatically switch to CE driving power, drive DNA fragmentation electrophoresis; Open fluoroscopic examination module simultaneously, detect the fluorescent signal of CE split tunnel; Signal is shown and preservation as data, and is output on other equipment.
The beneficial effects of the utility model are: compare with conventional genetic analysis flow process, this experiment is novel for Bacteria Detection, only need an equipment just can complete detection, have reduced the quantity of equipment, have reduced hardware acquisition cost; Improved the level of automation of analytic process, increased work efficiency; Avoid the transfer of sample between distinct device, simplified manual operation, reduced the risk of sample contamination; Micro-fluidic chip has reduced the consumption of sample and reagent, has reduced running cost; Device volume of the present utility model is little, can form handheld device, meets the detection needs of real time implementation, scene.
Accompanying drawing explanation
Fig. 1 is partial circuit block diagram of the present utility model, wherein 1-data processing and control module, and 2-electric power driving module, 4-fluoroscopic examination module, 5-temperature control module, 6-shows and interface module;
Fig. 2 is the structural representation of micro-fluidic chip, laminar substrate under 7-wherein, 8-PCR reaction channel, the separated liquid pool of 9-, waste liquid pool of 10-, No. bis-waste liquid pools of 11-, 12-CE split tunnel, 13-1 temperature control sheet, 14-2 temperature control sheet, 15-chip sample inlet pool;
Fig. 3 adopts the utility model to detect the result of porphyromonas gingivalis (Porphyromonas Gingivalis, Pg), wherein, (a) is the signal of the primer generation of PCR reaction, is (b) signal of the Pg bacterium specific sequence generation of 197bp.
Embodiment
For technique means, creation characteristic that the utility model is realized, reach object with effect is easy to understand, below in conjunction with the further elaboration the utility model of concrete diagram.
Referring to Fig. 1, a fast-bacteria-detection device based on micro-fluidic chip, comprises a bacteria detecting apparatus main body, and bacteria detecting apparatus main body is provided with a chip socket, one micro-fluidic chip is installed in chip socket, and micro-fluidic chip is formed by top substrate layer and the sealing-in of lower laminar substrate bonding; Lower laminar substrate comprises a PCR reaction channel, a CE split tunnel, and the port of PCR reaction channel and CE split tunnel is equipped with the groove as reagent pond; Top substrate layer comprises that one for filling the hole of reagent and arrangement electrode, and the position in hole is corresponding with the groove location of lower laminar substrate.
Bacteria detecting apparatus main body also comprises temperature control module 5, fluoroscopic examination module 4, electric power driving module 2, temperature control module 5, fluoroscopic examination module 4, electric power driving module 2 connection data are processed and control module 1, and data processing is connected with control module 1 and shows and interface module 6; Show with interface module 6 for showing detection data, or with other equipment connections such as flash memory device, hard disc apparatus, computer, output test data.Temperature control module 5 comprises for changing the semiconductor temperature-control sheet of the temperature of PCR reaction channel, also comprises for measuring the temperature sensor of the temperature of PCR reaction channel.Temperature sensor is electrical signal by temperature transition.Electric signal transmission, to data processing and control module, is produced the feedback control signal of semiconductor temperature-control sheet by the electrical signal of temperature according to predefined algorithm, make temperature control sheet can keep PCR reaction channel in constant design temperature.
Top substrate layer material and lower floor's baseplate material can be the baseplate materials of any one material in glass, quartz, polydimethylsiloxane (Polydimethylsiloxane, PDMS).Thereby the material that makes top substrate layer and lower laminar substrate has certain physical strength and good optical transmittance
PCR reaction channel comprises straight line portion and curved part, and straight line portion is arranged in parallel, and curved part is positioned at the two ends of straight line portion, and curved part connects two adjacent straight line portioies.The initiating terminal of PCR reaction channel is provided with the groove as sample inlet pool; The end of PCR reaction channel is provided with the groove as waste liquid pool.CE split tunnel is straight line passage.Or CE split tunnel is several beeline channels that coupled together by bending channel.The initiating terminal of CE split tunnel is provided with the groove as separated liquid pool; The end of CE split tunnel is provided with as waste liquid pool groove.Last straight line portion at PCR reaction channel end intersects with CE split tunnel; Intersection can be right-angled intersection, or double-T intersects.
Semiconductor temperature-control sheet is fitted in the one or two sides of micro-fluidic chip; When semiconductor temperature-control sheet is fitted in the one side of micro-fluidic chip, the quantity of semiconductor temperature-control sheet is 2～3, and the orientation of temperature control sheet is vertical with the orientation of the straight line portion of PCR reaction channel.Each temperature control sheet is across all straight line portioies of PCR reaction channel, and each straight line portion of PCR reaction channel is heated as by all temperature control sheets several humidity provinces that temperature is different, for sex change, extension and the annealing of PCR reaction.
Fluoroscopic examination module comprises excitation light source, optical delivery unit, photoelectric detector.Excitation light source produces the exciting light of narrower spectral bandwidth, and irradiates the detection window on CE split tunnel, excites the DNA fragmentation that contains fluorescence molecule to send fluorescence.Optical delivery unit is used for transmitting exciting light and collects fluorescence, and filtering wavelength equals to be less than the scattered light of exciting light.Photoelectric detector converts fluorescent signal to electrical signal, is transferred to data processing and control module.
Excitation light source can be the combination of photodiode, laser diode, broad spectrum light source and spike filter etc.Optical delivery unit can be freeboard, optical waveguides (for example optical fiber) and lens.Photoelectric detector can be photorectifier, phototriode, photomultiplier, charge coupled device etc.
Electric power driving module comprises PCR driving power and CE driving power; PCR driving power is placed on respectively the initiating terminal of PCR reaction channel with two and the electrode of end is connected.Be used for driving DNA molecular and pcr amplification product Continuous Flow thereof to cross PCR reaction channel.
CE driving power is placed on respectively the initiating terminal of CE split tunnel with two and the electrode of end is connected.For driving the DNA fragmentation after amplification to flow through CE split tunnel.
Embodiment 1. micro-fluidic chips.
The long 76mm of micro-fluidic chip, wide 22mm, thick 2mm, is formed by the sealing-in of upper and lower two-layer PDMS substrate bonding.Upper and lower laminar substrate thickness is 1mm, on lower laminar substrate, comprises PCR reaction channel, CE split tunnel and groove structure, and top substrate layer comprises pore structure, and position is corresponding with the groove structure of lower laminar substrate.
Referring to Fig. 2, the structure of lower laminar substrate 7.The cross-sectional dimension of PCR reaction channel 8 is 0.1mm * 0.1mm, and every two adjacent straight line portioies are one group, can complete a PCR reaction, has 30 groups.The cross-sectional dimension of CE split tunnel 12 is 0.1mm * 0.1mm, overall length 16mm.The straight line portion of PCR reaction channel tail end and the right-angled intersection of CE split tunnel, position, point of crossing is according to CE split tunnel initiating terminal 1.3mm.Chip sample inlet pool 15 is circular groove, diameter 5mm, dark 0.5mm.Separated liquid pool 9 is circular groove, diameter 4mm, dark 0.5mm.A waste liquid pool 10 and No. two waste liquid pools 11 are circular groove, diameter 4mm, dark 0.5mm.Two step method PCR is used No. 1 temperature control sheet 13 of two temperature control sheets and No. 2 temperature control sheets 14, and wherein, No. one temperature control sheet 13 temperature are higher, and for sex change, No. 2 temperature control sheet 14 temperature are lower, as extending and annealing.
During work, in the PCR reaction channel 8 of micro-fluidic chip, be filled with PCR reaction solution, CE split tunnel 12 is filled with parting liquid, and chip inserts proofing unit.The reagent that contains sample DNA 1 μ L is injected to chip sample inlet pool 15, open detection device, device completes all subsequent steps automatically.First, data processing and control module 1 order temperature control module 5 No. 1 temperature control sheet 13 of heating and No. 2 temperature control sheets 14, make the temperature of No. 1 temperature control sheet 13 reach 95 ℃, the temperature of No. 2 temperature control sheets 14 reaches 65 ℃, is kept afterwards the homo(io)thermism of No. 1 temperature control sheet 13 and No. 2 temperature control sheets 14 by temperature control module 5; Then, the PCR driving power part of data processing and control module 1 order electric power driving module 2 applies voltage between chip sample inlet pool 15 and a waste liquid pool 10, and chip sample inlet pool 15 is negative pole, and No. one waste liquid pool 10 is anodal; After 15 minutes, data processing and control module 1 order electric power driving module 2 switch to CE driving power part, between separated liquid pool 9 and No. two waste liquid pools 11, apply voltage, and separated liquid pool 9 is negative pole, and No. two waste liquid pool 11 is anodal; When CE driving power starts, data processing and control module 1 also order fluoroscopic examination module 4 to start, and detect the fluorescent signal of CE split tunnel 12; Fluorescent signal is transferred to data processing and control module 1 is processed and preserved, and can show result by showing with interface module 6, or is connected with miscellaneous equipment.
Embodiment 2. is used the fast-bacteria-detection device of the utility model PCR-based and capillary electrophoresis integrated chip to complete the rapid detection of porphyromonas gingivalis (Porphyromonas Gingivalis, Pg).Experimental result is referring to Fig. 3.
Use hygroscopic paper point (Tianjin DaYaDing Medical Appliance Co., Ltd) wiping gingival sulcus, obtain the gum seam liquid that contains oral cavity bacterium.The tip portion that speckles with gum seam liquid on hygroscopic paper point is immersed in to lysate PBS(U.S. Sigma-Aldrich company) in 3 minutes.At the PCR of micro-fluidic chip reaction channel 8, fill PCR reaction solution SpeedSTAR HS DNA Polymerase(Japan TAKARA company), CE split tunnel 12 is filled parting liquid (hydroxy ethyl fiber cellulose solution).Chip is inserted to proofing unit, at chip sample inlet pool 15, inject the lysate 1 μ L that contains oral cavity bacterium DNA, opening device, completes subsequent step automatically.Under the order of data processing and control module 1, temperature control module 5 makes No. 1 temperature control sheet 13 reach 95 ℃ and keep constant, makes No. 2 temperature control sheets 14 reach 65 ℃ and keep constant; Then start the PCR driving power of electric power driving module 2, make DNA molecular and pcr amplification product thereof along 8 continuous flows of PCR reaction channel, repeatedly, through the sex change district of 95 ℃ and the extension annealed zone of 65 ℃, complete pcr amplification reaction; After 15 minutes, automatically switch to CE driving power, at the two ends of CE split tunnel 12, apply the separation voltage that strength of electric field is 200V/cm, the module of fluoroscopic examination simultaneously 4 open detection; DNA fragmentation after separated passes through check point, excites generation fluorescence, after detecting and processing, is shown as and take the time as transverse axis, and the electrophoretogram that the fluorescence intensity of take is the longitudinal axis, referring to Fig. 3.In figure, the signal (a) that appears at approximately 0.75 minute comes from the primer in PCR liquid; The signal (b) that appears at approximately 1.4 minutes is the specific sequence that comes from the 197bp of Pg bacterium, shows to exist in sample Pg bacterium.In the present embodiment, from the sampling of oral cavity, to obtaining detected result, required time is in 20 minutes; The required reagent of whole testing process is approximately 200 μ L only.
More than show and described ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present utility model; do not departing under the prerequisite of the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.The claimed scope of the utility model is defined by appending claims and equivalent thereof.
Claims (10)
1. the fast-bacteria-detection device based on micro-fluidic chip, comprise a bacteria detecting apparatus main body, bacteria detecting apparatus main body comprises temperature control module, fluoroscopic examination module, electric power driving module, data processing and control module, described temperature control module, fluoroscopic examination module, electric power driving module all connect described data processing and control module, it is characterized in that, described bacteria detecting apparatus main body is provided with a chip socket, one micro-fluidic chip is installed in described chip socket, and described micro-fluidic chip is formed by top substrate layer and the sealing-in of lower laminar substrate bonding;
Described lower laminar substrate comprises a PCR reaction channel, a CE split tunnel, and the port of described PCR reaction channel and described CE split tunnel is equipped with the groove as reagent pond;
Described top substrate layer comprises that one for filling the hole of reagent and arrangement electrode, and the position in described hole is corresponding with the groove location of described lower laminar substrate.
2. a kind of fast-bacteria-detection device based on micro-fluidic chip according to claim 1, is characterized in that: described PCR reaction channel is a tortuous passage, comprises straight line portion and curved part;
Described straight line portion comprises a plurality of beeline channels of parallel arrangement, described curved part comprises a plurality of curved channels, the left end of a beeline channel is connected with the left end of adjacent next beeline channel by curved channel, and right-hand member passes through a curved channel and is connected with the right-hand member of an adjacent upper beeline channel;
The initiating terminal of described PCR reaction channel is provided with the groove as sample inlet pool; The end of described PCR reaction channel is provided with the groove as waste liquid pool.
3. a kind of fast-bacteria-detection device based on micro-fluidic chip according to claim 2, is characterized in that: described CE split tunnel is straight line passage, or as described in the tortuous passageway of PCR reaction channel formula;
The initiating terminal of described CE split tunnel is provided with the groove as separated liquid pool; The end of described CE split tunnel is provided with as waste liquid pool groove.
4. a kind of fast-bacteria-detection device based on micro-fluidic chip according to claim 3, is characterized in that: last straight line portion at described PCR reaction channel end intersects with described CE split tunnel; Described intersection is right-angled intersection, or double-T intersects.
5. according to a kind of fast-bacteria-detection device based on micro-fluidic chip described in claim 1,2,3 or 4, it is characterized in that: described temperature control module comprises that one for changing the semiconductor temperature-control sheet of the temperature of PCR reaction channel, also comprises for measuring the temperature sensor of the temperature of PCR reaction channel.
6. a kind of fast-bacteria-detection device based on micro-fluidic chip according to claim 5, is characterized in that: described semiconductor temperature-control sheet is fitted in the one or two sides of described micro-fluidic chip.
7. a kind of fast-bacteria-detection device based on micro-fluidic chip according to claim 6, it is characterized in that: when described semiconductor temperature-control sheet is fitted in the one side of described micro-fluidic chip, the quantity of described semiconductor temperature-control sheet is 2～3, and the orientation of temperature control sheet is vertical with the orientation of the straight line portion of PCR reaction channel.
8. a kind of fast-bacteria-detection device based on micro-fluidic chip according to claim 1, it is characterized in that: described fluoroscopic examination module comprises excitation light source, optical delivery unit, photoelectric detector, the range of exposures of described excitation light source covers described CE split tunnel.
9. a kind of fast-bacteria-detection device based on micro-fluidic chip according to claim 1, is characterized in that: described electric power driving module comprises PCR driving power and CE driving power; PCR driving power is placed on respectively the initiating terminal of PCR reaction channel with two and the electrode of end is connected;
Described CE driving power is placed on respectively the initiating terminal of CE split tunnel with two and the electrode of end is connected.
10. a kind of fast-bacteria-detection device based on micro-fluidic chip according to claim 9, is characterized in that: described data processing module also connects a demonstration and interface module.
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| CN201320892495.4U CN203807475U (en) | 2013-12-31 | 2013-12-31 | Quick bacteria detection device based on micro-fluidic chip |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104893954A (en) * | 2015-05-29 | 2015-09-09 | 上海交通大学 | Three-temperature-region channel embedded continuous flow laminated PCR (polymerase chain reaction) chip |
| CN105842324A (en) * | 2016-04-27 | 2016-08-10 | 河海大学常州校区 | Integrated micro-fluidic chip and working method thereof |
| CN107099598A (en) * | 2017-05-24 | 2017-08-29 | 济南市疾病预防控制中心 | A kind of method of the micro-fluidic integrated detection of bacterium |
| CN108088822A (en) * | 2016-11-21 | 2018-05-29 | 中国科学院大连化学物理研究所 | A kind of chip type light derivatization device of aflatoxin and sulfa drugs |
| CN112899149A (en) * | 2021-01-27 | 2021-06-04 | 上海理工大学 | Continuous flow microfluidic PCR real-time quantitative detection device and method |
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- 2013-12-31 CN CN201320892495.4U patent/CN203807475U/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104893954A (en) * | 2015-05-29 | 2015-09-09 | 上海交通大学 | Three-temperature-region channel embedded continuous flow laminated PCR (polymerase chain reaction) chip |
| CN105842324A (en) * | 2016-04-27 | 2016-08-10 | 河海大学常州校区 | Integrated micro-fluidic chip and working method thereof |
| CN105842324B (en) * | 2016-04-27 | 2018-10-23 | 河海大学常州校区 | A kind of integrated microfluidic chip and its working method |
| CN108088822A (en) * | 2016-11-21 | 2018-05-29 | 中国科学院大连化学物理研究所 | A kind of chip type light derivatization device of aflatoxin and sulfa drugs |
| CN108088822B (en) * | 2016-11-21 | 2020-05-26 | 中国科学院大连化学物理研究所 | Chip type light derivatization device for aflatoxin and sulfanilamide drugs |
| CN107099598A (en) * | 2017-05-24 | 2017-08-29 | 济南市疾病预防控制中心 | A kind of method of the micro-fluidic integrated detection of bacterium |
| CN112899149A (en) * | 2021-01-27 | 2021-06-04 | 上海理工大学 | Continuous flow microfluidic PCR real-time quantitative detection device and method |
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