CN203807461U - Buffer and perfusion system - Google Patents
Buffer and perfusion system Download PDFInfo
- Publication number
- CN203807461U CN203807461U CN201420249052.8U CN201420249052U CN203807461U CN 203807461 U CN203807461 U CN 203807461U CN 201420249052 U CN201420249052 U CN 201420249052U CN 203807461 U CN203807461 U CN 203807461U
- Authority
- CN
- China
- Prior art keywords
- cavity
- flexible pipe
- joint
- snubber
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000010412 perfusion Effects 0.000 title claims abstract description 17
- 239000007788 liquid Substances 0.000 claims description 53
- 239000012530 fluid Substances 0.000 claims description 26
- 238000002955 isolation Methods 0.000 claims description 9
- 230000002572 peristaltic effect Effects 0.000 claims description 7
- 239000007769 metal material Substances 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 3
- 238000007599 discharging Methods 0.000 abstract 4
- 230000008855 peristalsis Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 230000003139 buffering effect Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 210000002445 nipple Anatomy 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000009365 direct transmission Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- External Artificial Organs (AREA)
Abstract
The utility model relates to a buffer. The buffer comprises a cavity, wherein a feeding joint and a discharging joint are respectively connected with the top of the cavity and the bottom of the cavity, a flexible pipe is arranged inside the cavity, the feeding joint is connected with the upper end of the flexible pipe, the lower end of the flexible pipe extends to a part above the bottom of the cavity and is not communicated with the discharging joint, and an air release valve is also arranged at the top of the cavity. The utility model also relates to a perfusion system adopting the buffer. The perfusion system adopting the buffer comprises a feeding pipeline and a discharging pipeline, wherein the feeding pipeline and/or the discharging pipeline are/is connected with the buffer in series. The buffer and the perfusion system have the advantages that the problems of interference and jittering in the existing peristalsis technique are solved, waveform obtained by an experiment is clean, and data obtained by the experiment are accurate.
Description
Technical field
The utility model relates to for imparting knowledge to students and the Bioexperiment instrument field of scientific research, particularly, relates to a kind of snubber and perfusion system of using in perfusion operation of being applied to.
Background technology
Existing perfusion system, for example brain sheet perfusion system mostly adopts action of gravity to come fluid injection and discharge opeing, fluid injection under this mode need to be used special-purpose medicinal fluid bag or medicinal liquid bottle, and medicinal fluid bag need to be suspended to enough height and just can overcome pipe resistance, and then in inflow experimental tank, liquid flow velocity can be subject to medicinal fluid bag effect of altitude, wayward, thereby feed liquor flow is wayward, and the size of flux values is very not directly perceived; Suction port position during discharge opeing is non-adjustable, and flow is also non-adjustable, causes the liquid liquid level in experimental tank not regulate, affect the tissue activity in liquid, thereby cause experimental result not good, and discharge opeing need to be placed waste collecting device on experiment cell body side, obstruction experimental implementation.
In view of the above problems, there are now many use peristaltic pumps to the technology of perfusion or sucking-off liquid in experimental tank, its Flow-rate adjustment is directly perceived, easy, experimental tank inner liquid medicine liquid level is adjustable, experimental system layout is unrestricted, but because the wriggling of peristaltic pump can be brought a lot of interference into system, the liquid fluctuating in experimental tank is larger simultaneously, directly cause the experimental waveform data that detect to be forbidden, even test unsuccessful.
Utility model content
The technological deficiency that easily causes liquid level to disturb and fluctuate for overcoming existing perfusion system, the utility model discloses a kind of snubber.
Snubber described in the utility model, comprise cavity, described cavity top and cavity bottom are connected with respectively liquid inlet joint and fluid joint, described inside cavity is provided with flexible pipe, described flexible pipe upper end connects liquid inlet joint, flexible pipe lower end extends to cavity bottom top, and is not communicated with fluid joint, and described cavity top is also provided with vent valve.
Concrete, described flexible pipe lower end and cavity bottom distance are not more than 5 millimeters.
Preferably, described flexible pipe moves towards in inside cavity helically.
Preferably, described fluid joint is positioned at cavity bottom surface geometric centre, and described liquid inlet joint is positioned at non-central region, cavity top.
Preferably, described chamber outer wall is transparent material.
Preferably, described liquid inlet joint and/or draining connector are that metallic substance is made.
Preferably, cavity below is provided with annular isolation sheet, and described annular isolation sheet is positioned at below, flexible pipe lower end, and middle part has liquid outlet, and described fluid joint is positioned at liquid outlet below.
The utility model discloses a kind of perfusion system, comprises feed liquor pipeline and drain line, is in series with the snubber as described in above-mentioned any one in described feed liquor pipeline and/or drain line.
Preferably, described feed liquor pipeline and/or drain line are connected with peristaltic pump pipeline.
Snubber described in the utility model and perfusion system, overcome interference and liquid level jitter problem in existing wriggling technological method, makes that the waveform that obtains of experiment is clean, data are accurate.This snubber is in other interference of solve wriggling and can also filtering pipeline inner liquid medicine may bring into when self disturbing.
Accompanying drawing explanation
Fig. 1 is a kind of embodiment schematic diagram of the utility model;
Mark and corresponding component title in accompanying drawing: 1-cavity, 2-vent valve, 3-liquid inlet joint, 4-flexible pipe, 5-fluid joint, 6-annular isolation sheet.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the utility model is done to detailed description further, but embodiment of the present utility model is not limited to this.
Snubber described in the utility model, comprise cavity 1, described cavity top and cavity bottom are connected with respectively liquid inlet joint 3 and fluid joint 5, described inside cavity is provided with flexible pipe 4, described flexible pipe upper end connects liquid inlet joint, flexible pipe lower end extends to cavity bottom top, and is not communicated with fluid joint, and described cavity top is also provided with vent valve.
During use, the liquid that directly enters or carry into by peristaltic pump enters cavity by liquid inlet joint, by the flexible pipe being connected with liquid inlet joint, carry, because the cross section of cavity is much larger than flexible pipe cross section, inlet hydraulic is become suddenly large volume cavity and is absorbed, when cavity internal pressure is interrupted accumulation, by liquid outlet continuous strand slowly-releasing, put, thereby wriggling pressure is become to mild continuous pressure form, because flexible pipe 4 the other ends are not straight-through with liquid outlet, the liquid of coming in wriggling imports in cavity, the liquid level of having avoided water droplet to produce disturbs, the Clearance Flow of liquid under peristaltic forces effect becomes continuously mobile gently, the mechanical disturbance of having avoided wriggling and liquid level shake to form, vent valve 2 is equipped with at antifluctuator cavity 1 top, its effect is first to open vent valve 2 in the Preparatory work of experiment stage, at the feed liquor initial stage, the pressure entering is released from vent valve, simultaneously at the feed liquor initial stage, liquid level is very low, bottom liquid surface pressure is not enough, fluid pipeline has a fixed tube resistance simultaneously, the liquid entering can not drained from fluid joint, therefore can in snubber chamber, stockpile, when having a certain amount of liquid, close vent valve 2, the liquid entering afterwards will be discharged under pressure, and in antifluctuator cavity, remain a certain amount of liquid, avoid the liquid newly entering to be directly discharged, strengthened the slow releasing function of snubber.The liquid level situation of observing for convenience inside cavity, chamber outer wall preferably adopts transparent material.
Hose nipple can contact also and can not contact with cavity bottom, but conventionally apart from cavity bottom distance, should not be greater than 5 millimeters, too highly can form water droplet, thereby form new vibration interference, hose nipple also should keep certain distance apart from the inside liquid outlet of fluid joint, for example more than 5 millimeters, avoid the direct transmission of inlet hydraulic.For better strengthening buffering effect, to the trend of flexible pipe, can not adopt linear pattern, but bending is spiral-shaped.
The ambient interference and the circuit interference that for overcoming transmission static, bring, the ground wire that can contact with liquid in inside cavity setting, preferably select liquid inlet joint and fluid joint to make with metallic substance, while testing, these two joints are passed through to lead-in wire ground connection, and other independent grounding parts need not be set.The location optimization of two joints is set to fluid joint and is positioned at cavity bottom surface geometric centre, being convenient to chamber inner liquid medicine contacts with metal joint well, the center of gravity of Simultaneous Stabilization device, and liquid inlet joint is positioned at non-central region, cavity top, making two joints is on the one hand non-aligned position relationship, be beneficial to enhancing buffering effect, at cavity top, reserve larger space, vent valve 2 is conveniently set simultaneously.
To a kind of preferred arrangement of inside cavity as shown in Figure 1, for annular isolation sheet 6 is installed below cavity, described annular isolation sheet 6 is positioned at flexible pipe 4 belows, lower end to the utility model, and middle part has liquid outlet, and described fluid joint 5 is positioned at liquid outlet below.The fluid joint height of the height distance bottom of annular isolation sheet preferably remains within the scope of 1-5 millimeter, the lower end of flexible pipe is directly blocked in annular isolation sheet top, when hose length is longer, flexible pipe is extruded self-assembling formation curved shape, guarantee the distance apart from fluid joint simultaneously, realized good buffering effect.
When utilizing peristaltic pump perfusion technique to carry out brain sheet perfusion experiment, 1 snubber described in the utility model of only need to connecting respectively in fluid injection and/or drain line, based on above-described principle of work, be the steady fluid injection of feasible system and discharge opeing, in experimental tank liquid liquid level stabilizing can not shake, and filtering outside atmosphere is disturbed and circuit interference.
As mentioned above, can realize preferably the utility model.
Claims (1)
1. snubber, comprise cavity (1), it is characterized in that, described cavity top and cavity bottom are connected with respectively liquid inlet joint (3) and fluid joint (5), described inside cavity is provided with flexible pipe (4), and described flexible pipe upper end connects liquid inlet joint, and flexible pipe lower end extends to cavity bottom top, and be not communicated with fluid joint, described cavity top is also provided with vent valve (2).
2. snubber according to claim 1, is characterized in that, described flexible pipe (4) lower end and cavity (1) distance from bottom are not more than 5 millimeters.
3. snubber according to claim 1, is characterized in that, described fluid joint (5) is positioned at cavity bottom surface geometric centre, and described liquid inlet joint (3) is positioned at non-central region, cavity top.
4. snubber according to claim 1, is characterized in that, described flexible pipe (4) moves towards in inside cavity helically.
5. snubber according to claim 1, is characterized in that, described chamber outer wall is transparent material.
6. snubber according to claim 1, is characterized in that, cavity below is provided with annular isolation sheet (6), and described annular isolation sheet (6) is positioned at below, flexible pipe (4) lower end, and middle part has liquid outlet, and described fluid joint (5) is positioned at liquid outlet below.
7. snubber according to claim 1, is characterized in that, described liquid inlet joint (2) and/or draining connector (5) are made for metallic substance.
8. perfusion system, comprises feed liquor pipeline and drain line, it is characterized in that, is in series with the snubber as described in claim 1 to 6 any one in described feed liquor pipeline and/or drain line.
9. perfusion system according to claim 8, is characterized in that, described feed liquor pipeline and/or drain line are connected with peristaltic pump pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420249052.8U CN203807461U (en) | 2014-05-16 | 2014-05-16 | Buffer and perfusion system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420249052.8U CN203807461U (en) | 2014-05-16 | 2014-05-16 | Buffer and perfusion system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203807461U true CN203807461U (en) | 2014-09-03 |
Family
ID=51446244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420249052.8U Expired - Fee Related CN203807461U (en) | 2014-05-16 | 2014-05-16 | Buffer and perfusion system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203807461U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111849749A (en) * | 2020-03-31 | 2020-10-30 | 同济大学 | An efficient isolation system for adult small animal cardiomyocytes |
-
2014
- 2014-05-16 CN CN201420249052.8U patent/CN203807461U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111849749A (en) * | 2020-03-31 | 2020-10-30 | 同济大学 | An efficient isolation system for adult small animal cardiomyocytes |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140903 Termination date: 20150516 |
|
| EXPY | Termination of patent right or utility model |