CN203653566U - Candle type bioreactor - Google Patents
Candle type bioreactor Download PDFInfo
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- CN203653566U CN203653566U CN201320864221.4U CN201320864221U CN203653566U CN 203653566 U CN203653566 U CN 203653566U CN 201320864221 U CN201320864221 U CN 201320864221U CN 203653566 U CN203653566 U CN 203653566U
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- magnetic ring
- candle
- type filling
- reactor
- candle type
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- 238000011049 filling Methods 0.000 claims abstract description 61
- 239000007788 liquid Substances 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 230000008878 coupling Effects 0.000 claims description 4
- 238000010168 coupling process Methods 0.000 claims description 4
- 238000005859 coupling reaction Methods 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 238000003466 welding Methods 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 abstract description 9
- 210000004102 animal cell Anatomy 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 11
- 239000007789 gas Substances 0.000 description 8
- 238000003756 stirring Methods 0.000 description 6
- 239000002207 metabolite Substances 0.000 description 5
- 230000010261 cell growth Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 241000238634 Libellulidae Species 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a candle type bioreactor which is reasonable in structure and skillful in design, and is applicable to animal cell culture and microculture. The candle type bioreactor comprises an electrode, a candle type filling reaction tank, a machine base and a controller, wherein the candle type filling reaction tank comprises an upper cover, a candle type filling bed, an impeller combined piece, a shell and a lower bearing base; the upper cover is arranged on the top of the candle type filling reaction tank; the upper cover is provided with a plurality of interfaces and is mounted with the electrode; a deluge distribution plate, an upper liquid separating plate, a carrier cavity and a lower liquid separating plate are sequentially arranged on the candle type filling bed from upside to downside, and a carrier is accommodated in the carrier cavity; the impeller combined piece comprises blades, a rotating shaft and an inner magnetic ring; the machine base comprises a constant-temperature water bath tank and a motor; the motor is arranged at the bottom of the constant-temperature water bath tank; an outer magnetic ring base is arranged on the motor shaft; the outer magnetic ring base is sleeved on the lower bearing base; an outer magnetic ring is embedded in the outer magnetic ring base; the outer magnetic ring and the inner magnetic ring correspond in position, thus forming a structure that the inner magnetic ring and the outer magnetic ring are coupled and are mutually isolated by the lower bearing base.
Description
technical field:
The utility model relates to a kind of slot type bio-reactor that stirs, and is specifically a kind of candle formula bio-reactor, prepares biological products for zooblast, belongs to biological products Preparation equipment technical field.
background technology:
At present, the form of bio-reactor is many, and according to the feature of animal cell culture, the bio-reactor that is suitable for carrying out animal cell large-scale cultivation mainly contains stirred-tank reactor, hollow fiber reactor, airlift reactor and fluidized-bed reactor etc.
Stir slot type bio-reactor is the maximum one of application in cell cultures, and it had both been applicable to the suspension culture of zooblast, and the microcarrier that is also applicable to zooblast is cultivated and the cultivation of zooblast fixed bed.The cultivation of zooblast fixed bed has high-cell density, and substratum is changed simple, separates easily, and cell cultures is static, and cell has numerous features such as three-dimensional growing space.At present, with the basket stirring arm of U.S. NBS, chip carrier fixed bed is main flow.
summary of the invention:
The utility model provides a kind of candle formula bio-reactor, and this structure of reactor is reasonable, designs ingeniously, can be used for animal cell culture and microorganism culturing.
The purpose of this utility model realizes by following measures:
A kind of candle formula bio-reactor, comprises electrode, candle formula filling retort, support and controller;
Candle formula filling retort comprises upper cover, candle type filling bed, impeller sets component, shell and step, and candle formula filling retort top is provided with upper cover, is covered with multiple interfaces and described electrode is installed on this;
Described candle type filling bed is disposed with the grid distributor that drenches with rain, upper liquid separating plate, carrier chamber, lower liquid separatnig plate from top to bottom, and carrier is placed in carrier chamber;
Described cover top portion is installed upper cover, in shell, be provided with guide ring, guide ring bottom is connected with shell, the pod apertures that substratum passes through is convenient in axially and being radially equipped with of guide ring, candle type filling bed is arranged on guide ring top (with being threaded), guide ring top is provided with top chock, and outer casing bottom connects step, and lower bearing is placed in step;
Described impeller sets component comprises blade, rotating shaft and internal magnetic ring; Impeller sets component is located between upper and lower bearing support, and described blade is positioned at rotating shaft upper end, and rotating shaft lower end is set with internal magnetic ring, and this internal magnetic ring is placed in step;
Described support comprises constant temperature water bath and motor, motor is contained in water bath with thermostatic control trench bottom, outer magnetic ring seat is housed on motor shaft, outer magnetic ring cover for seat is contained on described step, outer magnetic ring seat is embedded with outer magnetic ring, outer magnetic ring is corresponding with the position of internal magnetic ring, forms inside and outside magnet ring coupling also each other by step isolation structure.
Described candle formula bio-reactor upper cover interface comprises four sensor interfaces and eight ports.
Described controller can be provided with four gas airing systems, peristaltic pump, color touch screen and control software.
Described candle formula bio-reactor is cylindrical, and its candle type filling bed is solid right cylinder.
Further design of the present utility model is:
The described grid distributor that drenches with rain is located at the top of candle type filling bed, and is positioned at the outer ring of candle type filling bed.Substratum overflows from candle type filling bed top and forms by the grid distributor that drenches with rain the gas in shape fluid and tank that drenches with rain and carry out balanced type exchange, and exchange area is large, can effectively increase the DO in substratum, and discharge foul smell, without deep layer air inlet.
The blade of described impeller is 2-8 sheet, adopts axial flow or centrifugal blade.
the utility model compared to existing technology tool has the following advantages:
1. the candle type filling bed of the utility model candle formula bio-reactor is solid right cylinder, and what be different from that the fixed bed of U.S. NBS adopts is ring-like fixed-bed structure form.
2. the utility model candle formula bioreactor culture base flow direction is upwards to be flowed by bottom, upwards flow in can form very stable laminar flow through the effect of liquid separatnig plate, whole candle type filling bed velocity flow profile is even, make carrier have a thrust upwards simultaneously, carrier is pressurized not, between carrier, be difficult for bonding agglomerating, being conducive to cell grows uniformly, easily cell metabolite is taken out of simultaneously, improve and make cell have a good growing environment, thereby improve cell cultures quality, avoid other filling beds to give carrier downward pressure because substratum flows downward, along with the prolongation of incubation time, carrier easily lumps, conglomeration, cause cell metabolite to assemble, cause Growth of Cells inhomogeneous, affect training quality.
3. the utility model candle formula bioreactor culture base flow direction is to flow through from bottom to top candle type filling bed, candle type filling bed top is provided with the grid distributor that drenches with rain, substratum overflows from candle type filling bed top and forms by the grid distributor that drenches with rain the gas in shape fluid and tank that drenches with rain and carry out balanced type exchange, exchange area is large, can effectively increase the DO in substratum, discharge foul smell, without deep layer air inlet.And U.S. NBS fixed bed substratum is to flow to filling bed bottom from top, need deep layer air inlet.
4. on the utility model candle type filling bed, device has upper and lower two skimmers, makes substratum in filling bed, form stable laminar flow, and distribution of culture medium and extremely low shearing force extremely are uniformly provided.
5. the utility model adopts built-in magnetic force coupling type stirring arm, and stirring rake, with outside isolated, can not pollute because of agitator.And the NBS fixed bed of the U.S. adopt be mechanical seal stirring arm, easily leak.
6. the utility model candle type filling bed, bed height, from 20 ~ 600mm capable of regulating, can form different fixed bed volumes.
7. the utility model candle type filling bed can filling be suitable for the strong adaptabilities such as the chip carrier being made up of various materials, ball-type carrier, microcarrier of cell cultures.
8. the utility model candle formula filling retort can be designed to disposablely or muptiple-use use, and form is multiple.
9. the utility model candle formula bio-reactor external diameter can form plurality of specifications from 100 ~ 1000mm, and Φ 100 lights up formula bio-reactor for laboratory, and Φ 200-1000 candle formula bio-reactor is for pilot scale and scale operation.
10. the utility model candle formula bio-reactor tank body is placed in constant temperature water bath, and temperature control is accurate.
11. the utility model constant temperature water baths and agitator motor combine together, and volume is little, easy to install.
accompanying drawing explanation:
Fig. 1 is the structural representation of the utility model candle formula bio-reactor.
Fig. 2 is the structural representation of candle formula filling retort.
Fig. 3 is the structural representation of upper cover.
Fig. 4 is the exploded view of candle formula filling retort.
Fig. 5 is the structural representation of candle type filling bed.
Fig. 6 is the exploded view of impeller sets component.
Fig. 7 is the structural representation of support.
In figure: 1-electrode; 2-candle formula filling retort; 3-support; 4-controller; 5-upper cover; 6-candle type filling bed; 7-impeller sets component; 8-shell; 9-step; 901-lower bearing; 10-guide ring; 1001-pod apertures; 11-top chock; The 12-grid distributor that drenches with rain; 13-upper liquid separating plate; 14-carrier chamber; Liquid separatnig plate under 15-; 16-rotating shaft; 17-blade; 18-internal magnetic ring; 19-outer magnetic ring; 20-constant temperature water bath; 21-motor; 22-outer magnetic ring seat; 23-gas-liquid inverting chamber.
embodiment:
Below in conjunction with accompanying drawing, the utility model will be further described:
As shown in Figure 1, the utility model candle formula bio-reactor comprises electrode 1, candle formula filling retort 2, support 3 and controller 4, in the constant temperature water bath 20 that candle formula filling retort 2 is arranged on support.Controller 4 can be installed control software, four gas airing systems, peristaltic pump and color touch screen, and then realize automatically and controlling.
As shown in Figure 2; Candle formula filling retort 2 comprises upper cover 5, candle type filling bed 6, impeller sets component 7, shell 8 and step 9, between shell 8 and candle type filling bed 6, is provided with gas-liquid inverting chamber 23, and candle formula filling retort 2 can adopt disposable rigid plastics to make; Or nonexpondable stainless material is made.
As shown in Figure 3, the upper cover 5 of this candle formula filling retort is provided with multiple interface F1, F2, F3, F4, and pH, DO, temperature, sugared electrode can be installed respectively; Also be provided with eight ports, can be respectively used to A substratum and add, B exhaust, C de-bubble, D adds soda acid, E inoculation, F sampling, G is settlement separate, H air inlet.
As shown in Figure 4, upper cover 5 is installed at shell 8 tops, step 9 is installed in shell 8 bottoms, forms a sealed vessel, is provided with guide ring 10 in the middle of shell 8, guide ring 10 and shell 8 ultrasonic weldings, the pod apertures 1001 that substratum passes through is convenient in axially and being radially equipped with of guide ring 10, and candle type filling bed 6 is threaded with guide ring 10 use, and guide ring 10 tops are also provided with top chock 11, shell 8 bottoms connect step 9, and lower bearing 901 is placed in step 9.
As shown in Figure 5, candle type filling bed 6 is disposed with the grid distributor 12 that drenches with rain, upper liquid separating plate 13, carrier chamber 14, lower liquid separatnig plate 15 from top to bottom, and carrier is placed in carrier chamber 14, and candle type filling bed 6 is solid cylinder.
As shown in Figure 6, impeller sets component comprises rotating shaft 16, blade 17 and internal magnetic ring 18; Blade 17 adopts axial flow or centrifugal blade, and blade arranges 2-8 sheet.Blade 17 is arranged on rotating shaft 16, and internal magnetic ring 18 is equipped with in rotating shaft 16 bottoms, and impeller sets component 7 is contained between upper and lower bearing support.In this step, set bearing and internal magnetic ring.
As shown in Figure 7, support 3 is provided with constant temperature water bath 20 and motor 21, and motor 21 is contained in constant temperature water bath 20 bottoms, and motor shaft is equipped with outer magnetic ring seat 22, and outer magnetic ring 19 is inlaid in outer magnetic ring seat 22.This outer magnetic ring 19 is corresponding with the position of internal magnetic ring 18, forms coupling and is isolated by step 9 each other.So just can realize outer magnetic ring 19 impeller subassemblies 7 and rotate, promote substratum at tank internal recycle.Avoid occurring that nutrient solution pollutes Seepage.
Constant temperature water bath 20 temperature control scope-5 ~ 100 ℃, normal temperature ~ 95 are ℃ available, and the constant temperature water bath 20 that candle formula filling retort 2 is put on support 3, can accurately control culture temperature, and it is controlled that temperature is adjustable, temperature-controlled precision ± 0.01 ℃.
principle of work of the present utility model:
On controller, start the motor on support, internal magnetic ring on outer magnetic ring impeller subassembly on motor shaft makes the rotation of impeller sets component, impeller sets component promotes substratum, substratum through lower liquid separatnig plate along candle type filling bed from bottom to top uniformly (laminar flow) flow through carrier to the cell supply nutrient being attached on carrier, and metabolite is taken out of, the grid distributor that drenches with rain through candle type filling bed top forms the shape that drenches with rain in the peripheral gas-liquid inverting chamber of candle type filling bed, carry out balanced exchange with gas in tank, provide O with this
2discharge foul smell, at the bottom of finally falling into tank, carry out culture tank internal recycle.Controller carries out relevant parameters control by electrode detection, carries out cell cultures.
the utlity model has following features:
1, candle formula bio-reactor of the present utility model is different from all now bio-reactors, its medium flow to be by bottom upwards flow, upwards flow in can form very stable laminar flow through the effect of liquid separatnig plate, whole candle type filling bed velocity flow profile is even, make carrier have a thrust upwards simultaneously, carrier is pressurized not, between carrier, be difficult for bonding agglomerating, being conducive to cell grows uniformly, easily cell metabolite is taken out of simultaneously, improve and make cell have a good growing environment, thereby improve cell cultures quality, avoid other filling beds to give carrier downward pressure because substratum flows downward, along with the prolongation of incubation time, carrier easily lumps, conglomeration, cause cell metabolite to assemble, cause Growth of Cells inhomogeneous, affect training quality.
2, substratum flows from bottom to top after candle type filling bed and to drench with rain at the bottom of grid distributor flows to tank through candle type filling bed top, now substratum forms the shape distributed flow that drenches with rain in container, at this moment substratum can be fully in gas-liquid inverting chamber with tank in gas (four gases or single gas) carry out gas-liquid exchange, O
2enter substratum, foul smell is discharged substratum, can effectively increase the DO in substratum, does not generally need to carry out deep layer air inlet.
3, because device has upper and lower liquid separatnig plate, make substratum in filling bed, form stable laminar flow, distribution of culture medium and extremely low shearing force extremely are uniformly provided.
4, the culturing process of total closed type bio-reactor from preculture to finished product is simple.This reactor can reach very high cell density, cultivates and has obvious advantage than the conventional stirred-tank formula of same volume.
5, this candle formula bio-reactor can form plurality of specifications from 25ML ~ 100L, simple to operate, saves time to reduce to produce and R&D costs.Be convenient to change between quick batch.And this candle formula bio-reactor can be realized linear amplification.
Claims (6)
1. a candle formula bio-reactor, is characterized in that: this candle formula bio-reactor comprises electrode, candle formula filling retort, support and controller;
Candle formula filling retort comprises upper cover, candle type filling bed, impeller sets component, shell and step, and candle formula filling retort top is provided with upper cover, is covered with multiple interfaces and described electrode is installed on this;
Described candle type filling bed is disposed with the grid distributor that drenches with rain, upper liquid separating plate, carrier chamber, lower liquid separatnig plate from top to bottom, and carrier is placed in carrier chamber;
Described cover top portion is installed upper cover, in shell, be provided with guide ring, guide ring bottom is connected with shell, the pod apertures that substratum passes through is convenient in axially and being radially equipped with of guide ring, candle type filling bed is arranged on guide ring top, guide ring top is provided with top chock, and outer casing bottom connects step, and lower bearing is placed in step;
Described impeller sets component comprises blade, rotating shaft and internal magnetic ring; Impeller sets component is located between upper and lower bearing support, and described blade is positioned at rotating shaft upper end, and rotating shaft lower end is set with internal magnetic ring, and this internal magnetic ring is placed in step;
Described support comprises constant temperature water bath and motor, motor is contained in water bath with thermostatic control trench bottom, outer magnetic ring seat is housed on motor shaft, outer magnetic ring cover for seat is contained on described step, outer magnetic ring seat is embedded with outer magnetic ring, outer magnetic ring is corresponding with the position of internal magnetic ring, the structure that forms inside and outside magnet ring coupling and isolated by step each other.
2. candle formula bio-reactor according to claim 1, is characterized in that: candle formula bio-reactor is cylindrical, and its candle type filling bed is solid right cylinder.
3. candle formula bio-reactor according to claim 1, is characterized in that: described candle formula bio-reactor upper cover interface comprises four sensor interfaces and eight ports.
4. according to the candle formula bio-reactor described in claim 1,2 or 3, it is characterized in that: described in the grid distributor that drenches with rain be located at the top of candle type filling bed, and be positioned at the outer ring of candle type filling bed.
5. candle formula bio-reactor according to claim 4, is characterized in that: the blade of described impeller adopts axial flow or centrifugal blade.
6. candle formula bio-reactor according to claim 1, is characterized in that: guide ring adopts ultrasonic welding mode to be connected with shell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320864221.4U CN203653566U (en) | 2013-12-26 | 2013-12-26 | Candle type bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320864221.4U CN203653566U (en) | 2013-12-26 | 2013-12-26 | Candle type bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203653566U true CN203653566U (en) | 2014-06-18 |
Family
ID=50919978
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320864221.4U Expired - Lifetime CN203653566U (en) | 2013-12-26 | 2013-12-26 | Candle type bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203653566U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104353142A (en) * | 2014-09-30 | 2015-02-18 | 南京比瑞生物科技有限公司 | Biological artificial liver reactor |
-
2013
- 2013-12-26 CN CN201320864221.4U patent/CN203653566U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104353142A (en) * | 2014-09-30 | 2015-02-18 | 南京比瑞生物科技有限公司 | Biological artificial liver reactor |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140618 |