CN203646369U - Lamplight lure based microbial pest control device - Google Patents

Abstract

The utility model discloses a device for preventing and controlling pests by microorganisms lured by light. The device for preventing and controlling pests based on light-induced microorganisms includes a solar panel, an automatic powder spraying device, an insect trap lamp tube, a storage battery and a support. The solar panel is fixed on the support top, and the automatic powder spraying device is suspended from the support top, and the insect trap lamp tube is installed in the center directly below the automatic powder spraying device. A dusting aerosol is installed in the automatic dusting device, and the content of the dusting aerosol is a microparticle powder and a propellant containing a microbial insecticide. In the utility model, light lure and microbial control are used synergistically, and the control area is enlarged by means of the migratory pests; and the virulent microbial insecticides are released according to the target pests to reduce the impact on beneficial insects. The utility model expands the traditional physical pest control technology and biological control technology, has low use cost, and is specially used for killing target pests, and is widely used in the fields of agricultural and forestry pest control and vector biological control.

Description

A device for preventing and controlling pests by microorganisms based on light lure

technical field

The utility model belongs to the field of pest control, in particular to a device for preventing and controlling pests by microorganisms lured by light.

Background technique

Light insecticidal technology is a technology that uses the phototaxis of insects to attract and kill pests, thereby preventing and controlling agricultural and forestry pests and sanitary pests. Different types of insects have different sensitivities to different bands of spectrum. The long-wave ultraviolet light with a wavelength of 320-400nm, which is invisible to humans, has a strong attracting power to hundreds of pests. Generally, the light source with a wavelength of 320-680nm and a spectral range covering long-wave ultraviolet light and visible light is called a broad-spectrum insect-attracting light source. The effective range of light traps is the circular area formed by the radius of the pests’ visible light source. The effective range of light traps is also related to the type and power of the light source. Generally, the control radius of insecticidal lamps is about 50m, and the effective control area is about 20 mu.

Insect trap lights can only lure and gather target flying pests, and they also need a supporting insecticidal device to have insecticidal effect. Generally, the insecticidal methods that are matched with the insect trap lamp include electric shock type, drowning type, inhalation type, sticking type and other physical control technologies. Lighting insecticidal technology is the most common physical control technology used in agricultural and forestry pest control, which can reduce the release of pesticides and have little impact on the environment.

Insect pathogenic microorganisms, also known as insecticidal microorganisms, are microorganisms that can cause disease and death of host insects, or whose metabolites can directly kill insects, including entomopathogenic bacteria, entomopathogenic fungi, insect viruses, entomopathogenic nematodes, etc. Including actinomycetes capable of producing insecticidal antibiotics. Entomopathogenic microorganisms are used in plant protection, animal husbandry and hygiene, and gradually form a new biological control method, that is, microbial control, and the corresponding biological pesticides are called microbial insecticides.

Entomopathogenic fungus grows on the surface of host insects, can invade the body from the surface of insects, and has contact killing effect. The fungal insecticides used more often include Beauveria bassiana, Metarhizium anisopliae, Penicillium, Paecilomyces, Pleurotus moths, and Erythrobacter. Compared with other microbial insecticides, fungal insecticides have a wider host range and stronger applicability. They mainly use contact killing and infect host insects by infecting the cuticle.

Viruses isolated from insects are divided into two categories, one can multiply in insects and cause insect disease, which is called entomopathogenic virus, and one kind of virus uses insects as a medium to infect other animals or plants, called arboviruses (arbovirus). As a biological control agent, entomopathogenic viruses, usually referred to as insect viruses (insect viruses), are specific, and their hosts are limited to insects, and do not infect vertebrates including humans. Insect viruses can cause insect epidemics in insect populations, especially high-density insect populations. Host insects are infected by feeding on insect viruses contaminated in the environment or by close contact with diseased insects. Insect viruses infected by host insects can be transmitted horizontally between individuals or vertically through eggs. Once a zoonosis breaks out in a host insect population, the entire population dies. Although some insect viruses cannot cause obvious diseases in host insects, they can affect the health of insect populations by putting them in a state of stress, reducing their harmfulness and keeping their density at a low level for a long time.

Insecticide formulations with entomogenic fungi and insect viruses as active ingredients can be wettable powder, powder, bait, oil, etc., and the application method is the same as that of chemical insecticides.

Using the phototaxis of insects to trap and kill agricultural pests with light is an important physical control measure and an important part of comprehensive control. At home and abroad, since the 1960s, lights have been used to predict and control various pests in cotton fields or other crop fields. Insect trap lights are also an important tool for monitoring insect migration, dispersal, occurrence period and occurrence amount, and are one of the effective measures for integrated pest management.

Commonly used light sources for attracting insects include electric lamps, gas lamps or oil lamps, and some mountainous areas also have direct fires as light sources for attracting insects. Since the 1970s, black light and other new insect traps have been widely used in the prediction and control of pests. In recent years, with the application of new technologies such as electronic technology and solar technology, the diversified development of insect traps has been promoted, and new energy-saving and efficient insect traps such as LED insect traps and solar insect traps have emerged. And widely used in agriculture, forestry, aquaculture and other fields.

Black light lamp is a kind of insect trap lamp widely used in agriculture at home and abroad. It belongs to special gas discharge lamp. Black light lamp is composed of high-voltage power grid insect killer and black light lamp. In the electric field, when the pest touches the power grid, a high-voltage arc is generated instantaneously, killing the pest. Because this kind of lamp mainly emits ultraviolet light and violet light, which are not sensitive to human beings, this kind of lamp is called a black light lamp. The frequency vibration insecticidal lamp uses the strong phototaxis and wave characteristics of pests to set the light wave within a specific range, using light at a short distance and using waves at a long distance. The lamp is equipped with a frequency vibration high-voltage power grid to kill the pests Bag drop, to reduce the amount of eggs dropped in the field, lower the population base of insects and play a role in the prevention and control of pests. The solar light trap adopts a maintenance-free independent solar power supply system to provide independent power supply, which avoids the inconvenience and harm caused by pulling AC wires in the field, and can easily adjust and control the spectrum and light intensity of the light source. According to the phototaxis characteristics of different pests, the intelligent controller provides targeted light sources with different wavelengths and light intensities to effectively kill pests, overcomes the defect of single performance of traditional light-induced pest killers, and improves the efficiency of pest control . The new double-wave lamp insect-attracting light source has the characteristics of short-wave black light and long-wave incandescent lamp. It is an insect-attracting lamp that can emit two long and short light waves at the same time. When the wave light emits light, the long light wave first lures the insects in the distance to the area near the light. Because the compound eyes of insects have poor tolerance to ultraviolet rays, they are dazzled and flick the lights. The double-wave lamp has a high amount of attracting insects, kills less natural enemies, and has a high response rate of insects in the field. Due to its inherent characteristics and pure light color, the new light source LED can use different spectra for different insects, so that we can accurately lock the target pests, kill pests to the greatest extent, and protect beneficial insects. At the same time, due to its high brightness, long range and low power consumption, it can trap a wider range of pests under the premise of saving energy.

The unique percutaneous invasion mechanism of entomogenic fungi makes them have a contact effect. Since the Russian Мечников first mass-proliferated Metarhizium anisopliae to control Austrian scarab (Anisop liaaustriaca) and sugar beet weevil (Cleonus punativ entris), Metarhizium anisopliae The main entomogenic fungi have become one of the important means for microorganisms to control pests. There are 51 registered fungal insecticides in the world, including 17 for Metarhizium anisopliae, and the dosage forms include powder, wettable powder, emulsion, oil, dry mycelia, microcapsules, etc. From production test to commercialization of Metarhizium anisopliae preparations, the most successful one is the Metarhizium anisopliae biopesticide developed by CABI Bioscience, which has been registered and put into production in Africa, and has been used on a large scale. It is used for the control of desert locust (Schistocerca gregaria); the control effect of this product reaches more than 90% after 10-15 days of application, and it becomes an effective means of locust biocontrol. The Australian Locust Biological Control Research Project (CSIRO) has developed a biopesticide of Metarhizium anisopliae, which is used to control large-scale grassland locusts, and is safe for non-target organisms such as natural enemies of locusts. Metarhizium anisopliae products (Bio-Path, Bio-Blast) from EcoScience in the United States are used to control cockroaches and termites; Metarhizium anisopliae products from Brazil and Venezuela (Metaquino, Cobican) are used to control sugarcane moths and so on. Brazil, Australia, Mexico, Japan and other countries have commercial preparations of Metarhizium anisopliae produced and widely used, mainly Metarhizium anisopliae, which is widely used in the control of crops, forests, sanitation and other pests. (Helicoverpa armigera), peach borer (Carosin aniponensis), corn borer (Ostrinia urnacalis), sugarcane stalk borer (Diatraea saccharalis), sweet potato leaf beetle (Colasposoma metallicum), litura litura (Prodenia litura), bamboo moth (Pantana phyllostachysae), Pseudophacopteron canarium, Monochamus alternatus, Paranthrenetabaniformis, Endolimus punctatus, Blattella germanica, Black-winged soil termite (Odontotermes formosanus) and other pests have obvious control effect. my country has developed oils, powders, wettable powders and floating agents for locusts, coconut leaf beetles (Brontispalongissima), and rice water weevils (Lissorhoptrus oryzophilus). Utilizing Metarhizium anisopliae to control the cocoa beetle, the number of spores used per plant of the powder reaches the order of 10 ⁹ , the control effect exceeds 80% after 15 days of application, and the lethality rate of the wettable powder reaches 84% after 7 days of application. One of the main techniques of the pest cocoa beetle.

In 1975, the U.S. Environmental Protection Agency approved the registration of the first baculovirus insecticide that could be used to control cotton pests. At present, in Brazil, more than 1 million hectares of land use baculovirus to control soybean pests every year; in Europe, another A baculovirus is used to control pests on fruit trees. In the South Pacific, a naked virus (Nudivirus) is widely used to control the rhinoceros beelte on coconut trees. In my country, as of September 2013, 11 viral insecticides have been registered for biological control of agricultural, forestry, grassland and sanitary pests.

Due to the short light radiation distance, the existing light insecticidal technology has a limited control range and cannot distinguish between pests and beneficial insects. Both pests and beneficial insects are killed at the same time, so the cost of use is high and the insect community in the control area is greatly affected. Difficult to apply on a large scale. Microbial control technology can be applied on a large scale, but the application technology, especially the technology of pest forecasting is very demanding. Premature application of microbial insecticides, due to the small number of target host pests, it is difficult for insect pathogenic microorganisms to increase and spread with the help of host populations, and microbial control factors will soon fail to degrade in nature. The best time to apply microbial insecticides is missed, the population density is already too high, and it takes about a week for the host pests to infect the microbial control factors and die. purpose of biological control.

Utility model content

The purpose of the utility model is to overcome the shortcomings and deficiencies of the prior art, and provide a device for preventing and controlling pests based on microorganisms lured by light.

The purpose of this utility model is achieved through the following technical solutions:

A device for preventing and controlling pests based on light-induced microorganisms, that is, an "attraction-infection-release" device, including a solar panel, an automatic powder spraying device, an insect trap lamp tube, a storage battery and a bracket. The insect trap lamp tube is installed in the center directly below the automatic powder spraying device. A dusting aerosol is installed in the automatic dusting device, and the content of the dusting aerosol is a microparticle powder and a propellant containing a microbial insecticide. Preferably, the solar panel is fixed on the top of the support, and the automatic powder spraying device hangs from the top of the support.

The power generated by the solar panels is preferably no less than 25 watts.

The storage battery guarantees that the device can operate normally under the weather condition of continuous cloudiness for 3 days.

The power of the insect trap lamp is preferably not less than 8 watts, and the opening time is preferably not less than 3 hours per day.

The type of the microbial insecticide is selected according to the target pests, and its active ingredients are mainly contact killing, and have selective toxicity to the target pests. The recommended active ingredients are entomogenic fungi such as Metarhizium anisopliae, Beauveria bassiana, and insect viruses. The fineness of the particulate powder containing microbial insecticide is not more than 600 mesh.

The automatic powder spraying device can be triggered by timing or by infrared rays; the automatic powder spraying device is equipped with an infrared or timing trigger device and a control chip. The infrared triggering device sets the threshold of the activity intensity of pests under the insect trap lamp. When the threshold is reached, the first spraying is started, and the subsequent spraying is stopped after several times of timing spraying. After 24 hours, the spraying operation is carried out according to this process. The timing trigger device determines the initial spraying time according to the time when the target pests flick the lights, and the subsequent timing spraying stops after several times, and after an interval of 24 hours, the spraying operation is carried out according to this process.

The effective control area of the above-mentioned light-attracted microbial pest control device is determined according to the average flying distance of the target pests. Control its effective control area radius between 50m and 100m, which is equivalent to 12 mu to 47 mu.

The above-mentioned device for controlling pests based on light-induced microorganisms should be activated before the target pest damage starts, and continue to be turned on until the harvest of crops is completed or the threat of sanitary pests disappears.

The above-mentioned device for controlling pests based on light-induced microorganisms can control different target pests by adjusting the active ingredients of microbial pesticides or/and the wavelength of the light source of the insect-attracting lamp tube, which improves the control efficiency and reduces the impact on beneficial insects or non-target insects. Impact.

The above-mentioned device for preventing and controlling pests based on light-induced microorganisms lures and gathers target pests through light sources or spectral waves, and applies insect-pathogenic microorganisms with selective virulence to the target pests at fixed points, causing the target pests to carry insects such as insect-borne fungi or insect viruses. Microbes spread among populations and cause insect epidemics, thereby achieving the purpose of pest control. This method of using a light source or spectral wave that is sensitive to pests and has strong attracting power to lure and gather target pests, and then implement microbial control at a fixed point, is referred to as "pull-infect-release technology (PIR)". The PIR device releases an insect-sensitive light source in a specific spectral range to attract agricultural and forestry pests and sanitary pests to the surroundings of the PIR device. Through infrared triggering or timed spraying, it sprays the surface of the target pests that gather and fly around the insect trap lamp. Particle dry powder of insect pathogenic microorganisms such as fungi and insect viruses. Target pests carry entomopathogenic microorganisms and return to their populations, where they subsequently become infected. Due to the high population density of pests and the close contact between individuals due to inhabiting, feeding, mating and other behaviors, the infected insect pathogenic microorganisms will cause insect epidemics in the target pest population in a short period of time, resulting in the decline of individual harm ability and population density, and the pest population will be destroyed. Controlled to the extent of insufficient hazard. Specific target pests can be controlled by releasing insect-pathogenic microorganisms with selective virulence. Insect pathogenic microorganisms spread far beyond the control area of ordinary insecticidal lamps by means of the flying and habitat habits of pests.

Compared with the prior art, the utility model has the following advantages and effects:

The utility model uses the light lure technology and the microbial control technology synergistically, and doubles the control area of the light insect killing technology by means of the migratory pests, and releases the microbial control factors with selective toxicity according to different target pests to kill the target pests. , avoid killing beneficial insects by mistake, reduce the impact on beneficial insects, and solve the disadvantages of conventional light insecticidal technology, such as limited control range, killing all pests and beneficial insects, and only killing adults.

The "attraction-infection-release" device of the utility model can be set in the field or other places where pests are dense, and through the long-term fixed-point continuous release of insect-causing microorganisms, the target pest population can be continuously infected with insect epidemics, and the pest population density can be controlled for a long time. the extent of the hazard.

The utility model expands the traditional physical pest control technology and biological control technology, has low use cost, and is specially used for killing target pests, and is widely used in the fields of agricultural and forestry pest control and vector biological control.

Description of drawings

Fig. 1 is a structural schematic diagram of the "attraction-infection-release" device; wherein, 1 is a solar panel, 2 is an automatic dusting device, 3 is an insect trap lamp, 4 is a microbial insecticide, 5 is a storage battery, and 6 is a support.

曲线为对照区的防效-处理时间曲线，

曲线为对照区的密度下降率-处理时间曲线。Fig. 2 is the control effect statistics of the utility model to rice planthopper

The curve is the control effect-treatment time curve of the control area,

The curve is the density decrease rate-treatment time curve of the control area.

曲线为对照区的防效-处理时间曲线，

曲线为对照区的密度下降率-处理时间曲线。Fig. 3 is the monitoring statistics of the utility model to the control effect of mosquitoes by the method of trapping mosquitoes and oviraptors

The curve is the control effect-treatment time curve of the control area,

The curve is the density decrease rate-treatment time curve of the control area.

曲线为对照区的防效-处理时间曲线，曲线为对照区的密度下降率-处理时间曲线。Fig. 4 is the monitoring statistical diagram of the mosquito trap lamp of the utility model to the control effect of mosquitoes,

The curve is the control effect-treatment time curve of the control area, The curve is the density decrease rate-treatment time curve of the control area.

Detailed ways

The utility model will be further described in detail below in conjunction with the embodiments and accompanying drawings, but the implementation of the utility model is not limited thereto.

Example 1

An "attraction-infection-release" device, its structural schematic diagram is shown in Figure 1, including solar panel 1, automatic powder spraying device 2, insect trap lamp tube 3, storage battery 5 and bracket 6 and other components. Solar panel 1 is fixed on support 6 tops, and support 6 tops hangs automatic powder spraying device 2, and insect trap lamp tube 3 is installed in the center directly below automatic powder spraying device 2. Dust spraying aerosol is installed in the automatic powder spraying device 2, and the powder spraying aerosol is convenient to change, and content is the particle powder and the propellant containing microbial insecticide 4.

The generating power of the solar panel 1 is not less than 25 watts, and the storage battery 5 guarantees that the device can operate normally under continuous cloudy weather conditions for 3 days.

The power of the insect trap lamp 3 is not less than 8 watts, and the opening time is not less than 3 hours every day.

The type of microbial insecticide 4 is selected according to the target pests, and its active ingredients are mainly contact killing, and have selective toxicity to the target pests. The recommended active ingredients are entomogenic fungi such as Metarhizium anisopliae, Beauveria bassiana, and insect viruses. The fineness of the particulate powder containing microbial insecticide is not more than 600 mesh.

The automatic powder spraying device 2 can be a timing trigger powder spray, or an infrared trigger powder spray. Infrared ray or timing trigger device and control chip are installed in the automatic dusting device 2 . The infrared trigger sets the threshold of the activity intensity of the pests under the insect trap lamp. When the threshold is reached, the first spray will be started, and the subsequent spraying will stop after a few times of timing. After an interval of 24 hours, the spraying operation will be carried out according to this process. The timing trigger determines the initial spraying time based on the time when the target pests flick the lights, and the subsequent timing spraying will stop after a few times, and after an interval of 24 hours, the spraying operation will be carried out according to this process.

The effective control area of the "attraction-infection-release" device is determined according to the average flying distance of the target pests. Control its effective control area radius between 50m and 100m, which is equivalent to 12 mu to 47 mu.

The "attraction-infection-release" device should be released before the target pest damage starts, and it will continue to open until the crop harvest is completed or the threat of sanitary pests disappears.

According to different crops and target pests in the control area, the "attraction-infection-release" device can realize the prevention and control of different target pests by adjusting the light source wavelength of microbial insecticides or/and insect trap lamps, improve the control efficiency, and reduce the Effects of beneficial or non-target insects.

The control of embodiment 2 paddy rice planthopper

Rice planthopper, a common name for insect pests of the family Delphacidae of the order Homoptera (Homoptera), damages rice and other crops by piercing and sucking plant juices, including brown planthopper (Nilaparvata lugens), white-backed planthopper (Sogatella furcifera) and gray planthopper (Laodelphax striatellus), etc. Rice planthopper is an important pest of rice in China, and it has occurred continuously in various places in recent years. In the initial stage of rice damage, many irregular brown spots appeared on the stalks. When the damage was serious, the whole plant would wither and expand into pieces, causing the whole field of rice plants to fall and wither, severely reducing yield, and posing a serious threat to rice production.

Methamidophos, an insecticide widely used in the control of rice planthoppers, has been banned in China since January 1, 2007, due to its high toxicity and high residue characteristics, and its strong lethality to beneficial organisms in rice fields. Used in agriculture. At present, the commonly used insecticides for the control of rice planthoppers mainly include imidacloprid, buprofezin, secbucarb and isoprocarb, but with the large-scale and high-frequency use of imidacloprid, the resistance of rice planthoppers to it has increased rapidly. Various places reflect that its effect on rice planthopper is not as good as before.

Rice planthoppers have strong phototaxis. From August 10 to September 10, 2013, a field experiment was carried out using the "lure-infection-release" technology to verify the control effect of the utility model on rice planthoppers.

Materials and Methods

Experimental site: The experimental site was selected in the Jiangxia rice field in Wuhan, and the single-cropping late rice varieties were planted. Select 6 plots, each plot is square, with a side length of about 200m. The interval between the plots is not less than 100m, and 3 treatment plots and 3 control plots are made respectively, and the intervals are distributed.

The wavelength of the insect trap lamp and the active ingredient of the microbial insecticide: the wavelength of the insect trap lamp is 365nm. The Metarhizium anisopliae selected as the active ingredient of the microbial insecticide is a strain isolated and purified from dead termites in ant nests in the wild by Wuhan University. After morphological, physiological, biochemical, and molecular biological identification, the strain is a strain of Metarhizium anisopliae. A new variant was named Metarhizium anisopliae var.dcjhyium (CCTCC collection number M206077, Genbank accession number DQ288247), referred to as Metarhizium anisopliae Wuhan strain Lj01. The active ingredient content of the applied agent is 5 billion spores/g, and the dosage form is microparticle dry powder (DP) with a fineness of 600 mesh.

Prevention and control method: The "attraction-infection-release" device is installed in the center of the field in the treatment area, and the lower end of the insect trap lamp is 1.5m away from the ground. According to the habit of flicking the lights of rice planthoppers, turn on the lights at 19:00, and the first spraying time of the timing powder spraying device is 20:00, and the duration of each spraying of microbial insecticide is 5s, and the spraying rate is 0.3g/s～0.5g/s. Spray 1 time at an interval of 1 hour, spray 3 times in a row, and end the treatment of the day. The treatment was sequentially performed daily for 21 days.

Investigation method: 3 consecutive days before the treatment, 7d, 14d, 21d and 28d after the start of the treatment. The treatment area is centered on the "attraction-infection-release" pest control device, and the circular area within a radius of 100m and the control area population density of rice planthoppers. The survey method is to investigate 30 points in each treatment area or control area by parallel jumping method, with 2 clusters in each point, and a total of 60 clusters are investigated. Use a 40cm×30cm white porcelain plate to brush a layer of engine oil, place it obliquely in the middle and lower part of the rice, quickly pat the rice plant 2 times, and then quickly lift it up to check the number of rice planthoppers. Sampling by the same investigation method before and after spraying, investigating the number of residual live insects, and counting the number of residual insects in 100 caves.

According to the survey results, the insect population decline rate and corrected control effect of each treatment area were calculated in chronological order.

Insect population reduction rate = [(number of live insects before treatment - number of live insects after treatment)/number of live insects before treatment] × 100%,

Corrected control effect = [(the reduction rate of the insect population in the chemical treatment area-the reduction rate of the insect population in the blank control area)/(1-the reduction rate of the insect population in the blank control area)]×100%.

Using SPSS analysis software, Duncan's new multiple range method (DMRT) was used to analyze the variance of the experimental data.

Result analysis

From 7d to 28d after applying the pesticide, the rice in the treatment area grew normally, and there was no obvious abnormality in leaf color, plant height, etc., and no leaf chlorosis or deformity occurred, indicating that the "attraction-infection-release" technology is safe for rice.

The test results show that the 365nm wavelength lures and releases 5 billion spores per gram of Metarhizium anisopliae Wuhan strain Lj01 particulate powder, which is effective in controlling late rice planthoppers. The control effect reached 81.3% in 14 days, and continued to 28 days, with a control effect of 90.6% (Figure 2 ). The control radius reaches 100m, and the equivalent area reaches 47 mu, while it is usually reported that the control area of ordinary light insecticide is only in the range of 10 mu to 20 mu. However, due to the continuous release of microbial pesticides at fixed points in the field, the target pests have a process of migrating and spreading. Within 7 days of implementing the utility model, there is no significant control effect, so it is necessary to implement "attraction-infection- release" technology.

The control of embodiment 2 mosquitoes

Mosquito is a general term for species of Mosquitoidae of the order Diptera of Insecta. Mosquito species closely related to humans include common species of Anopheles, Aedes and Culex, which not only sting human blood , and spread malaria, dengue fever, Japanese encephalitis and many other insect-borne diseases, it is the vector that is the most harmful to humans. Mosquito control in outdoor environments usually adopts adult mosquito space spraying insecticides, removal of mosquito breeding sites, screen doors, screens, mosquito nets and other physical barriers, personal protection and other measures. During the high-incidence season of mosquitoes, it is often difficult to implement treatment measures for breeding sites. Frequent space spraying of chemical insecticides is a short-term control measure that pollutes the environment and endangers health. It is not suitable for large-scale and high-intensity implementation. As the threat of emerging and re-emerging mosquito borne diseases such as dengue fever and chikungunya fever transmitted by mosquitoes continues to rise, the environment outside human settlements requires an efficient, environmentally friendly and sustainable mosquito control technology.

Mosquitoes have strong phototaxis, and mosquitoes are also infected by a variety of insect pathogenic microorganisms. From June 20 to July 20, 2013, the "attraction-infection-release" technology was used to carry out field experiments on mosquito control to verify the practicality of this method. New type of control effect on mosquitoes.

Materials and Methods

Experimental site: The experimental site is selected in a residential area in Hongshan District, Wuhan. The area is a 7-story old-fashioned residential building with a brick-concrete structure that has been built for about 20 years. Mainly, in summer and autumn every year, residents complain more about mosquito bites.

The wavelength of the insect trap lamp and the active ingredient of the microbial insecticide: the wavelength of the insect trap lamp is 365nm, and the active ingredient of the microbial insecticide is Metarhizium anisopliae, which is a strain isolated and purified from dead termites in the wild ant nest by Wuhan University. According to the identification of morphology, physiology, biochemistry and molecular biology, the strain is a new variant of Metarhizium anisopliae var.dcjhyium (CCTCC preservation number M206077, Genbank accession number is DQ288247), referred to as Metarhizium anisopliae Wuhan strain Lj01. The content of active ingredients in the sprayed agent is 5 billion spores/g, and the dosage form is microparticle dry powder (DP) with a fineness of 600 mesh.

Prevention and control methods: The "attraction-infection-release" device is installed on the west side of the main road of the community, in the green area between two buildings, and the lower end of the insect trap lamp is 1.5m away from the ground. According to the mosquito's habit of flicking the lamp, turn on the light at 18:00, and the first spraying time of the timing powder spraying device is 18:30. Spray 1 time at an interval of 1 hour, spray 3 times in a row, and end the treatment of the day. The treatment was sequentially performed daily for 21 days.

The control area is the east side of the main road of the residential area, the green area between the two buildings, and the linear distance of 200m from the treatment area, with a building blocking in the middle.

Investigation method: use mosquito ovitrap method and insect trap method, respectively, for 3 consecutive days before the treatment, and investigate the treatment area within 100m from the "attraction-infection-release" device on the 7th, 14th, 21d and 28th days after starting the treatment Mosquito densities in circular area and control areas.

The mosquito ovitrap method is to set 20 mosquito ovitraps (produced by Guangdong Dongnan Industrial Co., Ltd.) in each treatment area or control area, mainly to detect the density of Aedes mosquitoes, and set them up on the 7th day before the start of the experiment. Put a piece of φ9cm qualitative filter paper into each mosquito ovirap, and add overnight tap water, the water level should not be higher than the surface of the filter paper. After each inspection, the water and filter paper should be changed. Mosquito and egg traps are placed in the outdoor green belt. When deploying, the distance between mosquito and egg traps should be ensured, and one should be placed every 10m to 15m.

Calculation of mosquito-egg trapping index:

LTS-M02B "Kung Fu Xiaoshuai" photocatalytic mosquito and fly trapping device (manufactured by Wuhan Jixing Environmental Protection Technology Co., Ltd.) was selected as the monitoring tool for the mosquito trap method. Since 7 days before the start of the experiment, it will be monitored every 7 days. The weather (wind force above level 5) will be postponed. Set up 4 hanging lamp points in an external environment far away from interfering light sources and sheltered from the wind, located in the four directions of southeast, northwest, respectively, and the straight-line distance between each lamp and the "attraction-infection-release" device exceeds 80m. The light source of the mosquito trap light is 1.5m away from the ground, turn on the power supply 1 hour before sunset, turn on the mosquito trap lamp to trap mosquitoes, take it back 1 hour after sunrise on the 2nd day, identify the species, count, and calculate the density (piece/lamp).

The mosquito trap adopts the method of late setting and early harvesting. The main mosquito species to be monitored is Culex, and the density index is calculated by Culex:

Mosquito killing effect = [(density before treatment - density after treatment) / density before treatment] × 100%,

Corrected mosquito control effect = [(insect control effect in chemical treatment area - density decrease rate in blank control area) / (1 - density decrease rate in blank control area)] × 100%.

Using SPSS analysis software, Duncan's new multiple range method (DMRT) was used to analyze the variance of the experimental data.

Result analysis

From 7d to 28d after applying the pesticide, the vegetation in the treatment area grew normally without obvious abnormalities, and there was no chlorosis or deformity of the leaves of the green plants, which indicated that the "attraction-infection-release" technology treatment was safe for urban garden plants.

The test results show that the 365nm wavelength lures and releases 5 billion spores per gram of Metarhizium anisopliae Wuhan strain Lj01 microparticle powder, which has a good effect on controlling mosquitoes in urban residential communities. Mosquito-ovitrap test, the control effect reached 73.5% at 14 days, and 91.2% at 28 days (Figure 3); as determined by the mosquito trap method, the control effect reached 57.8% at 14 days, and 82.9% at 28 days (Figure 4).

This experiment confirmed that the control range of "attraction-infection-release" technology is more than 80m in a straight line, and the control area of ordinary outdoor light insecticidal lamps is within 50m. However, due to the continuous release of microbial insecticides at fixed points in the external environment, mosquitoes need to migrate and infect the process. Within 7 days of implementing the utility model, there is no significant control effect, so it is necessary to implement "attraction-infection- release" technology.

The above-mentioned embodiment is a preferred implementation mode of the present utility model, but the implementation mode of the present utility model is not limited by the above-mentioned embodiment, and any other changes, modifications and substitutions made without departing from the spirit and principle of the present utility model , combination, and simplification, all should be equivalent replacement methods, and are all included in the protection scope of the present utility model.

Claims (10)

1. a device for the microbial control insect based on light trapping, is characterized in that: comprise solar panels, automatic powder spraying device, light trap pipe, accumulator and support; Light trap pipe be arranged on automatic powder spraying device under center; The aerosol that dusts is installed in automatic powder spraying device, and the aerosol content that dusts is particulate pulvis and the propellant containing microorganism insecticide.

2. the device of the microbial control insect based on light trapping according to claim 1, is characterized in that: described solar panels are fixed on cantilever tip, the cantilever tip automatic powder spraying device that dangles.

3. the device of the microbial control insect based on light trapping according to claim 1, is characterized in that: described solar panels generated output is not less than 25 watts.

4. the device of the microbial control insect based on light trapping according to claim 1, is characterized in that: described accumulator ensures to connect under cloudy 3 days weather conditions, and this device can run well.

5. the device of the microbial control insect based on light trapping according to claim 1, is characterized in that: the power of described light trap pipe is not less than 8 watts, and opening time every day is not shorter than 3 hours.

6. the device of the microbial control insect based on light trapping according to claim 1, is characterized in that: the active ingredient of described microorganism insecticide is selected according to target pest; The fineness of the described particulate pulvis containing microorganism insecticide is not more than 600 orders.

7. the device of the microbial control insect based on light trapping according to claim 1, is characterized in that: described automatic powder spraying device is that clocked flip dusts or infrared rays triggers.

8. the device of the microbial control insect based on light trapping according to claim 1, is characterized in that: effective control area of the device of the described microbial control insect based on light trapping is determined according to the distance of on average circling in the air of target pest.

9. the device of the microbial control insect based on light trapping according to claim 1, it is characterized in that: the time of discharging of the device of the described microbial control insect based on light trapping be target pest harm initial before, continue to be opened into that crop harvesting completes or sanitary insect pest threatens and disappears.

10. the device of the microbial control insect based on light trapping according to claim 1, it is characterized in that: the device of the described microbial control insect based on light trapping is by adjusting the active ingredient of microorganism insecticide or/and the optical source wavelength of light trap pipe is realized the different target pest of control.

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