CN203422367U - System detector capable of detecting food components rapidly based on micro electrode array - Google Patents
System detector capable of detecting food components rapidly based on micro electrode array Download PDFInfo
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Abstract
The utility model relates to an electrochemical food safety detection device, and particularly relates to a system detector capable of detecting food components rapidly based on a micro electrode array. A host machine of the system detector is internally provided with a chip insertion groove; one surface of the detector host machine is provided with a button region and a display region; a biological sensor chip mainly comprises a comparison liquid bag and a groove chip clip; the groove chip clip is matched with the chip insertion groove. According to the utility model, effective components in food can be detected rapidly, and can be toxic and harmful substances, such as aflatoxin, or supplemental effective components, such as ginseng soap alkali; meanwhile, the problems that the detection operation of food additives and toxic and harmful substances is complex, and long time is spent are solved; the system detector provided by the utility model is easy to prepare, simple and convenient to operate and test, high in flexibility and great in specificity, and has short response time.
Description
Technical field
The utility model relates to a kind of Electrochemical Detection food security device, specifically a kind of system detector of the fast detecting food component based on microelectrode array.
Background technology
Effective constituent is chemistry, biology, and the term of materia medica widespread use, refers in a kind of potpourri, the composition that biosome metabolism or chemical reaction are worked.In pharmacy, effective constituent: there is certain biologic activity in natural drug, can represent the chemical composition of its effect.As having, Sanedrine relievings asthma, spasmolysis; Glycyrrhizic acid has anti-inflammatory, antiallergy, and the effect for the treatment of gastric ulcer, being construed to is respectively the representative effective constituent of Chinese medicine Chinese ephedra and Radix Glycyrrhizae.Therefore it is quite necessary can fast detecting going out the limited component of food, and for some food, some food component poisonous and harmful, such as our edible vegetable seed sesame oil, in general fuel-displaced sesame oil, generally all contain the aflatoxins of certain component, this mycin has harmful to human body, so the World Health Organization (WHO) has proposed new definition for food security.
Food security refers to that food is nontoxic, harmless, meets the nutritional requirement that should have, and health is not caused to any acute, subacute or chronic hazard.According to the definition of the World Health Organization (WHO), food security is " public health problem of poisonous and harmful substances to Health Impact in food ".Food security is also to inquire into guarantee food hygiene and edible safety in the processes such as food processing, storage, sale, reduces disease risk, takes precautions against interdisciplinary fields of food poisoning.The closest diet problem of specialty research daily life relation, its effect can not be underestimated.Job operation, process and device used in Food Science and Engineering research food industry production, she is the design basis of food production process and equipment, relates to each subjects such as chemistry, physics, agronomy, biological chemistry, microbiology, chemical engineering, Biochemical Engineering, mechanical engineering, human nutrition and food hygiene, environmental improvement and engineering.An importance of Food Science and Engineering is introduce and use chemical engineering unit operation, and concurrent spread becomes food engineering unit operations, thereby promotes food industry to the future development of extensive, serialization and robotization.The same chemical engineering of development of Food Science and Engineering, bioengineering are closely related, and its developing direction is that new packing means and equipment improve Food Packaging technology, improve food preservation performance and shelf life; Improve boiling technology, aseptic packaging technology; Study rational energy saver to reduce the cost of frozen food; The optimization of food engineering unit operations, robotization and computer application.Although being young technology-oriented discipline ，Dan Ta modern societies, Food Science and Engineering become already the outstanding feature of economic development, civilization degree raising.Following development trend is to use for reference the development result of gene technology, develops that nutrition is more abundant, more delicious taste, more scientific food of shelf-life.
Therefore detecting fast and effectively adjuvant not up to standard in food and poisonous and hazardous material is the current important development problem for whole food security industry.
Summary of the invention
The purpose of this utility model is to provide a kind of system detector of the fast detecting food component based on microelectrode array.
The utility model solves its above-mentioned technical matters and adopts following technical scheme: a kind of system detector of the fast detecting food component based on microelectrode array, it is mainly configured with: instrument host, key zone, viewing area, comparison liquid capsule, biologic sensor chip, groove chip card, chip reads probe, analyzer, chip insertion groove, puncture probe, comparison electrode, chip tectorial membrane, comparison liquid permeable membrane, filtrate basement membrane, yellow film, three color base films, sensor base plate, green film, red membrane, test fluid syringe needle, chip top cover inside groove film, inner tank mouths, injector, test fluid, in described instrument host, a place is provided with chip insertion groove, instrument host one side is provided with key zone, viewing area, biologic sensor chip is mainly comprised of comparison liquid capsule, groove chip card, and described groove chip card and chip insertion groove match, chip insertion groove inside upper part is provided with comparison electrode, and bottom is provided with chip and reads probe, and chip reads probe mount and is fixed on analyzer, in instrument host, a place is also provided with and punctures probe, punctures probe location corresponding on comparison liquid capsule,
Described comparison liquid capsule is to be composited by chip tectorial membrane, comparison liquid permeable membrane, filtrate basement membrane, three color base films, sensor base plate; Three color base films are compounded with yellow film, green film, red membrane;
Described chip tectorial membrane inner layer surface is provided with inner tank mouths, and inner tank mouths is rolled up one deck chip top cover inside groove film;
Described test fluid is placed in injector, and test fluid splashes in chip top cover inside groove film by test fluid syringe needle.
Above-mentioned biologic sensor chip is disposable abandoning.
Above-mentioned yellow film, green film, red membrane are semi-permeable diaphragm.
Above-mentioned instrument host one place is provided with data-out port.
The beneficial effects of the utility model: can fast detecting go out the active principle in food, this kind of component can be poisonous and harmful substance, such as aflatoxins; Also can be the effective constituent of augmenting, such as ginseng soap alkali; Solved simultaneously food additives, poisonous and harmful substance have been detected to complicated operation, and need the long problem of spended time, the microfluid mixer chip that provide that a kind of easy preparation and test operation are simple and convenient, highly sensitive, high specificity, response time are short, can quantitatively detect food additives, poisonous and harmful substance content, has realized the direct-detection to food beneficiating ingredient, poisonous and harmful substance content.The utility model is of great significance in food industry and the equal tool of field of environment protection.
Accompanying drawing explanation
Fig. 1 is the system detector main machine structure schematic diagram of a kind of fast detecting food component based on microelectrode array of the utility model.
Fig. 2 is the system detector chip insertion groove of a kind of fast detecting food component based on microelectrode array of the utility model and the structural representation of biologic sensor chip.
Fig. 3 is each film released state schematic diagram of system detector comparison liquid capsule of a kind of fast detecting food component based on microelectrode array of the utility model.
Fig. 4 is each film merging phase schematic diagram of system detector comparison liquid capsule of a kind of fast detecting food component based on microelectrode array of the utility model.
Fig. 5 is that the system detector comparison liquid capsule of a kind of fast detecting food component based on microelectrode array of the utility model is punctured the schematic diagram that state splashes into test fluid.
Fig. 6 is the figure that shows many concentration determinations result of the present embodiment 2.
1-instrument master in figure, 2-key zone, 3-viewing area, 4-comparison liquid capsule, 5-biologic sensor chip, 6-groove chip card, 7-chip reads probe, 8-analyzer, 9-chip insertion groove, 10-punctures probe, and 11-compares electrode, 12-chip tectorial membrane, 13-comparison liquid permeable membrane, 14-filtrate basement membrane, the yellow film of 15-, 16-tri-color base films, 17-sensor base plate, 18-green film, 19-red membrane, 20-test fluid syringe needle, 21-chip top cover inside groove film, 22-inner tank mouths, 23-injector, 24-test fluid.
Embodiment
Below in conjunction with accompanying drawing 1-6, embodiment of the present utility model is made a detailed explanation.
Embodiment: a kind of system detector of the fast detecting food component based on microelectrode array, it is mainly configured with: instrument host 1, key zone 2, viewing area 3, comparison liquid capsule 4, biologic sensor chip 5, groove chip card 6, chip reads probe 7, analyzer 8, chip insertion groove 9, puncture probe 10, comparison electrode 11, chip tectorial membrane 12, comparison liquid permeable membrane 13, filtrate basement membrane 14, yellow film 15, three color base films 16, sensor base plate 17, green film 18, red membrane 19, test fluid syringe needle 20, chip top cover inside groove film 21, inner tank mouths 22, injector 23, test fluid 24, in described instrument host 1, a place is provided with chip insertion groove 9, instrument host 1 one side is provided with key zone 2, viewing area 3, biologic sensor chip 5 is mainly comprised of comparison liquid capsule 4, groove chip card 6, and described groove chip card 6 matches with chip insertion groove 9, chip insertion groove 9 inside upper part are provided with comparison electrode 11, and bottom is provided with chip and reads probe 7, and chip reads probe 7 bases and is fixed on analyzer 8, in instrument host 1, a place is also provided with and punctures probe 10, punctures probe 10 positions corresponding on comparison liquid capsule 4,
Described comparison liquid capsule 4 is to be composited by chip tectorial membrane 12, comparison liquid permeable membrane 13, filtrate basement membrane 14, three color base films 16, sensor base plate 17; Three color base films 16 are compounded with yellow film 15, green film 18, red membrane 19;
Described chip tectorial membrane 12 inner layer surfaces are provided with inner tank mouths 22, and inner tank mouths 22 is rolled up one deck chip top cover inside groove film 21;
Described test fluid 24 is placed in injector 23, and test fluid 24 splashes in chip top cover inside groove film 21 by test fluid syringe needle 20.
Described biologic sensor chip 5 is disposable abandoning.
Described yellow film 15, green film 18, red membrane 19 are semi-permeable diaphragm.
Described instrument host 1 one places are provided with data-out port.
For the configuration component of test fluid 24, do the explanation of a description below:
By confirming to be examined, amount of solution reduce erroneous judgement disconnected when measuring, can realize the ease of Use that correctness is high and an existing step immuno-array method has.It is the porous material that use can be wetting that a so-called step is exempted from remorse Array Method, by being examined the interpolation of solution, start the immunoassay of measuring, it is the mensuration system of having utilized antigen-antibody reaction, in common immunoassay, the clean operation that B/F separation etc. are necessary, one step immuno-array method is the process of look grammer carrier of soaking into by being examined solution, realizes the mensuration system of B/F separation.Conventionally all reagent, in drying regime, utilizes and is examined solution wetted when measuring.Because user's basic measurement operation is just added and is examined the operation of solution, so be called a step immuno-array method.The thing that serves as a mark is generally used gold colloid, latex, also uses magnetic particle, enzyme, metallic colloid etc.
Configuration component embodiment 1:
Be manufactured on the immune look grammer developer layer in broadband of the compound of the line that contains anti-hCG-p antibody immobilization in high and steepization cellulose membrane and anti-hCG-a antibody and gold colloid.Utilize space to form material and make this immuno-array method developer layer, divide formation clearance portion being examined solution addition portion, manufacture immuno-array test piece.Sample contains antibody immobilization portion, the labelled reagent maintaining part of the compound that be examined in contrast to this side that solution more contacts, contains anti-hCG-a antibody and gold colloid.These samples are performed as follows manufacture.
A. the preparation of immuno-array method developer layer
First prepare, with phosphate buffer solution dilution, to adjust the anti-hcG-p antibody-solutions of concentration, use solution bleeder that this antibody-solutions is coated on nitrocellulose filter.Can on nitrocellulose filter, obtain like this detecting the line of the antibody immobilization of use.After this nitrocellulose filter is dry, be immersed in the Tris-Hcl buffer solution of the skimmed milk that contains 1%, shake lentamente 30 minutes.After 30 minutes, film is transferred in Tris-HCI buffer solution groove, slowly vibrated 10 minutes, in other Tris-HCI buffer solution groove, slowly vibrate again 10 minutes, purification membrane afterwards.After carrying out 2 clean operations as above, from detergent remover, take out film, in drying at room temperature.Utilizing in 0.01% gold chloride, the solution of 100 ℃ in backflow adds citric acid solution to prepare gold colloid.Gold colloid is cooling after continuous backflow 30 minutes again, utilizes the solution of potassium carbonate of 0.2M to be adjusted into pH9.In this gold colloid solution, adding hCG-a antibody, carry out the stirring of several minutes, only add afterwards 10%BsA(bovine serum albumin(BSA)) to make it be finally 1% amount to pH value of solution 9, stirs, and is made into antibody-gold glue compound (labelled antibody) solution.Thereafter utilize the centrifuging of 20000G50 minute, separation marking antibody, is suspended in clean damping fluid (1%BSA phosphate buffer) separately, carries out afterwards centrifuging, the separated labelled antibody of cleaning.
With clean buffer solution, be suspended this labelled antibody, with filtrator, filter, be made into 1/10th of initial gold colloid solution amount, at 4 ℃ of storages. then, the labelled antibody solution of preparation is like this placed on solution bleeder, be coated on the position separated with antibody immobilization position on anti-hCG-p antibody immobilization desciccator diaphragm, make afterwards film dry.On fixed film, can obtain like this labelled antibody and keep position.Can complete like this immuno-array method responding layer, be developer layer.
B. the making of immuno-array method test piece:
On the sensor base plate 17 that the white PET that is 0.8mm at thickness makes, paste and know the epidemic disease Array Method developer layer that the above is made, with the width cut-out of 2.8mm.After cut-out, each sheet of immuno-array method, from labelled antibody retaining part to terminal part, is rolled to the oolemma that thickness is 100 μ m.In the central authorities of the top of the oolemma of not rolling part, pick up the stacked formation material in space in advance with airport of making of transparent PET that pastes thickness 100 μ m, form clearance portion (wide 2.0mm, the high 0.8mm of long 6.00mx).Make like this test piece of immuno-array method.
C. the preparation of test solution
By add the hCG solution of concentration known in people's urine, be mixed with the hCG solution of various concentration known.
D. measure
The urine that contains hCG 15 μ 1 that concentration is prepared drop on sensor base plate 17, on sensor base plate 17, form drop.The drop of formation is contacted to the clearance portion of immune look grammer test specimen, it is imported in clearance portion.The drop importing launches to process along water accepting layer direction, makes it carry out antigen-antibody reaction, the chromogenic reaction in being fixed portion.Here use the colour developing situation of reflection-type spectrophotometric determination add sample 5 minutes in this test specimen part after, computing colour developing degree.First on immuno-array test specimen, add and contain that hcG concentration is respectively 100,1000, the urine of the hCG of 10000U/l, launches processing.Then, the colour developing situation of the antibody immobilization portion on the test specimen of the urine correspondence of each hCG of use reflection-type spectrophotometric determination.Utilize the absorbance of the wavelength of reflection-type spectrophotometric determination 520nm, bring the detection line of the relation of the demonstration hCG concentration made in advance and absorbance into.
Configuration component embodiment 2:
Whole blood CRP is quantitatively manufactured on the immuno-array method test piece of the labelled reagent of the compound of slightly changing the Immobilized reagents portion of containing the anti-CRP antibody A of immobilization in cellulose membrane and keeping anti-CRP antibody B and gold colloid with the making of test piece.This immuno-array method test piece be included in the more approaching interpolation of Immobilized reagents portion being immobilized than antibody be examined solution expansion starting point compound part, that contain anti-CRP antibody B and gold colloid region labelled reagent and detect thing maintaining part.This immunity look language test piece is manufactured as follows.
A. the preparation of immuno-array method test piece
Preparation is diluted with phosphate buffer solution, adjusts the anti-CRP antibody A solution of concentration.Use solution bleeder that this antibody-solutions is coated on nitrocellulose filter.Can on nitrocellulose filter, obtain the line of the antibody immobilization of Immobilized reagents portion like this.After this nitrocellulose filter is dry, be immersed in the Tris-HCI buffer solution of the skimmed milk that contains 1%, shake lentamente 30 minutes.After 30 minutes, film is transferred in Tris-Hcl buffer solution groove, slowly vibrated 10 minutes, in other Tris-HCI buffer solution groove, slowly vibrate again 10 minutes, purification membrane afterwards.After carrying out the clean operation on knowing for 2 times, from liquid bath, take out film, in drying at room temperature.Utilizing in 0.01% gold chloride, the solution of 100 ℃ in backflow adds citric acid solution to prepare gold colloid.Cooling after continuous backflow 30 minutes again, utilize solution of potassium carbonate O.2M, in being adjusted into the described gold colloid solution of pH9, add anti-CP antibody B to carry out the stirring of several minutes, only adding afterwards 10%BSA(bovine serum albumin(BSA)) to make it be finally 1% amount to pH value of solution 9, stir, be made into the antibody one gold colloid compound (labelled antibody) of detection material.Thereafter utilize labelled antibody solution described in the centrifuging of 20000G50 minute, the separated labelled antibody of cleaning, is suspended in clean damping fluid (1%BSA phosphate buffer), carries out afterwards centrifuging, the separated separation marking antibody of cleaning.With clean buffer solution, be suspended this labelled antibody, with the filtrator of 0.8 micron, filter, be made into afterwards 1/10th of initial gold colloid solution amount, at 4 ℃ of storages.Described gold colloid labelled antibody solution is placed on solution bleeder, coating, the immobilized line being from anti-CRP antibody immobilization A desciccator diaphragm separates, by being examined solution, adds the position relationship that beginning direction is followed successively by labelled antibody, immobilized line, makes afterwards film carry out vacuum freezing and is dried.The responding layer carrier on like this can being fixed film with labelled reagent.Then, it is on the sensor base plate 17 that forms of the white PET of 0.8mm that the responding layer carrier that contains labelled reagent preparing is sticked on to thickness, with the width of 5.0mm, cuts off.After cut-out, each sheet, from labelled antibody retaining part to terminal part, is rolled to thickness and is the oolemma of 100 microns.In the central authorities of the top of the oolemma that does not have, rolls part, the stacked space in advance with airport of making of transparent PET of pasting 100 microns of thickness forms material, forms clearance portion (the long 12.0mm of wide 5.0mmx, high 0.8mm).On space forms material, forming and preventing from advance detecting the wall that the liquid of thing maintaining part leaks.And, described space forms material and makes as follows, lights the potassium chloride solution that 2 μ 1 are made into 1.8M in advance on per unit area, utilizes afterwards liquid nitrogen freezing immediately, carry out freeze drying, manufacture thus and there is potassium chloride with the space formation material of the shrinking agent maintaining part of drying regime maintenance.Manufacture like this test piece of immuno-array method.
B. the preparation of sample
It is 45% that human blood using interpolation EDTA.ZK as anticoagulant is mixed with hematocrite value.By add the CRP solution of concentration known in this blood, be mixed with the various blood that contains concentration known CRP.
C. the mensuration of the colour developing degree in test piece
The whole blood preparing described in use, considers actual blood sampling situation, makes it fill the syringe that 20ml volume is used, and need not measure the whole blood that contains CRP of certain volume, adds in right amount and detects thing maintaining part.After interpolation, be examined solution and be inhaled into clearance portion.To water suction portion direction, launch to process again, make it carry out antigen-antibody reaction, carry out the chromogenic reaction of antibody immobilization portion.Add sample 5 minutes on this biology sensor after, utilize reflection absorbance measurement instrument to measure its colour developing situation.
The whole blood that contains 0.lmg/dl, 1.omg/d1,3.omg/d1 as blood plasma concentration is directly added on biology sensor in right amount by described 20ml syringe, launches to process.The colour developing situation of the Immobilized reagents part on biology sensor corresponding to the blood of each CRP concentration is utilized) measure.Measure the absorbance of the wavelength of 635nm, according to individual CRP concentration mapping.This is illustrated in to Fig. 6.Fig. 6 is the figure that shows many concentration determinations result of the present embodiment 2.Transverse axis shows the CRP concentration of using commercially available determinator to measure.Here commercially available determinator is used the reagent of latex immune agglutination method.The longitudinal axis shows the absorbance obtaining in addition.So-called detection line refers in the region that CRP concentration rising absorbance rises relatively, conventionally utilize in advance the solution that is examined of concentration known to calculate, then, after locating and being examined solution, from the absorbance obtaining, can calculate the calculating formula that this unknown is examined the CRP concentration solution.In addition, the biology sensor that the biological device in the present embodiment 2 is used nitrocellulose and the such Array Method material by Porous carrier forms arbitrarily of glass fiber filter paper to form.The biology sensor that material forms like this uses any measuring principle of for example antigen-antibody reaction class, has to analyze to detect certain predetermined substance, carries out qualitative or quantitative function.In the present embodiment 2, use is provided with the biology sensor of labelled reagent and Immobilized reagents portion on same case cellulose membrane, with the material different with nitrocellulose, for example on the Porous carrier of Non-woven fabrics, load is held the reagent that serves as a mark of labelled reagent, even if be placed on supporter also without any problem.The label that forms labelled reagent is used the existing example of gold colloid, also can be coloring material, fluorescent material, phosphorus, luminescent substance, redox materials, enzyme, nucleic acid, endoplasmic reticulum, so long as produce the material of some variation before and after reaction, can use.
As the solution that is examined of measuring, for example there are water or aqueous solution, urine, blood, blood plasma, serum, the body fluid such as saliva, solid and powder or make liquid of gas dissolution etc., its purposes is uroscopy and the pregnant inspection of shaking, examination of water, just check, soil analysis, food analysis etc. the existing example narration of analyte CRP matter (CRP) in addition, also has in addition antibody, immunoglobulin (Ig), hormone, the protein such as enzyme and skin and protein derivatives and bacterium, virus, Mycophyta, mycoplasma, the infectious substance that parasite and these products and one-tenth thereof grade, curative and medicine and the tumor marker of abusing medicine etc.Even it is also no problem that concrete human chorionic gonadtropin (hcG), corpus luteum hormone (LH), thyroid-stimulating hormone (TSH), filter born of the same parents form hormone, first shape parent glandular spines swash hormone, adrenal cortex stimulation hormone, estradiol, prostate specific antigen, hepatitis B surface antibody, myoglobins, CRP, cardiac troponin, HbAlc/ protein etc.In addition, also can be used in the aspects such as the environmental analyses such as examination of water, soil analysis and food analysis.According to knowing front described form, can realize easy fast, the mensuration of high sensitivity high-performance, high correctness.The possibility of utilizing in industry is known the above.
The biology sensor that the utility model relates to as a kind of at immuno-array method, the biology sensor that uses in a step immuno-array method etc., be useful, be particularly suitable as for towards food component, judge and easy and simple to handleization of general family etc. and the desired situation of fast under measure.
Claims (4)
1. the system detector of the fast detecting food component based on microelectrode array, it is mainly configured with: instrument host (1), key zone (2), viewing area (3), comparison liquid capsule (4), biologic sensor chip (5), groove chip card (6), chip reads probe (7), analyzer (8), chip insertion groove (9), puncture probe (10), comparison electrode (11), chip tectorial membrane (12), comparison liquid permeable membrane (13), filtrate basement membrane (14), yellow film (15), three color base films (16), sensor base plate (17), green film (18), red membrane (19), test fluid syringe needle (20), chip top cover inside groove film (21), inner tank mouths (22), injector (23), test fluid (24), it is characterized in that: instrument host (1) Nei Yichu is provided with chip insertion groove (9), instrument host (1) one side is provided with key zone (2), viewing area (3), biologic sensor chip (5) is mainly comprised of comparison liquid capsule (4), groove chip card (6), and described groove chip card (6) matches with chip insertion groove (9), chip insertion groove (9) inside upper part is provided with comparison electrode (11), and bottom is provided with chip and reads probe (7), and chip reads probe (7) base and is fixed on analyzer (8), in instrument host (1), a place is also provided with and punctures probe (10), punctures probe (10) position corresponding on comparison liquid capsule (4),
Described comparison liquid capsule (4) is to be composited by chip tectorial membrane (12), comparison liquid permeable membrane (13), filtrate basement membrane (14), three color base films (16), sensor base plate (17); Three color base films (16) are compounded with yellow film (15), green film (18), red membrane (19);
Described chip tectorial membrane (12) inner layer surface is provided with inner tank mouths (22), and inner tank mouths (22) is rolled up one deck chip top cover inside groove film (21);
Described test fluid (24) is placed in injector (23), and test fluid (24) splashes in chip top cover inside groove film (21) by test fluid syringe needle (20).
2. the system detector of a kind of fast detecting food component based on microelectrode array according to claim 1, is characterized in that described biologic sensor chip (5) is for disposable abandoning.
3. the system detector of a kind of fast detecting food component based on microelectrode array according to claim 1, is characterized in that described yellow film (15), green film (18), red membrane (19) are semi-permeable diaphragm.
4. the system detector of a kind of fast detecting food component based on microelectrode array according to claim 1, is characterized in that described instrument host (1) one place is provided with data-out port.
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| CN201320577354.3U CN203422367U (en) | 2013-09-17 | 2013-09-17 | System detector capable of detecting food components rapidly based on micro electrode array |
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| CN201320577354.3U CN203422367U (en) | 2013-09-17 | 2013-09-17 | System detector capable of detecting food components rapidly based on micro electrode array |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105043453A (en) * | 2015-08-31 | 2015-11-11 | 福州市台江区振斌高效电磁聚能科技研究所 | Food safety inspection device |
| CN108931639A (en) * | 2017-05-25 | 2018-12-04 | 浙江清华长三角研究院萧山生物工程中心 | The chip and reagent of cortisol detection in a kind of saliva |
| CN111349555A (en) * | 2018-12-21 | 2020-06-30 | 成都万众壹芯生物科技有限公司 | Digital PCR amplification device based on micropore array chip and use method thereof |
| CN114460149A (en) * | 2020-11-09 | 2022-05-10 | 陈文亮 | Biochip detection device, biosensor platform, manufacturing method and application thereof |
-
2013
- 2013-09-17 CN CN201320577354.3U patent/CN203422367U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105043453A (en) * | 2015-08-31 | 2015-11-11 | 福州市台江区振斌高效电磁聚能科技研究所 | Food safety inspection device |
| CN105043453B (en) * | 2015-08-31 | 2017-11-10 | 长乐芯聚电子科技研究所 | Food safety inspection device |
| CN108931639A (en) * | 2017-05-25 | 2018-12-04 | 浙江清华长三角研究院萧山生物工程中心 | The chip and reagent of cortisol detection in a kind of saliva |
| CN111349555A (en) * | 2018-12-21 | 2020-06-30 | 成都万众壹芯生物科技有限公司 | Digital PCR amplification device based on micropore array chip and use method thereof |
| CN114460149A (en) * | 2020-11-09 | 2022-05-10 | 陈文亮 | Biochip detection device, biosensor platform, manufacturing method and application thereof |
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