CN203220855U - Tangential flow ultrafiltration device - Google Patents
Tangential flow ultrafiltration device Download PDFInfo
- Publication number
- CN203220855U CN203220855U CN 201320213578 CN201320213578U CN203220855U CN 203220855 U CN203220855 U CN 203220855U CN 201320213578 CN201320213578 CN 201320213578 CN 201320213578 U CN201320213578 U CN 201320213578U CN 203220855 U CN203220855 U CN 203220855U
- Authority
- CN
- China
- Prior art keywords
- unit
- sample
- led control
- pump
- sample jar
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000108 ultra-filtration Methods 0.000 title claims abstract description 33
- 239000012528 membrane Substances 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims description 13
- 238000013019 agitation Methods 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 4
- 230000002706 hydrostatic effect Effects 0.000 claims description 3
- 238000005530 etching Methods 0.000 claims description 2
- 238000005086 pumping Methods 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000003760 magnetic stirring Methods 0.000 abstract 2
- 238000012544 monitoring process Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 description 10
- 238000001914 filtration Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 238000000926 separation method Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229920006221 acetate fiber Polymers 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 239000010836 blood and blood product Substances 0.000 description 1
- 229940125691 blood product Drugs 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000009295 crossflow filtration Methods 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000012065 filter cake Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 238000010010 raising Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Images
Landscapes
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The utility model discloses a tangential flow ultrafiltration device comprising a shell, an LED (Light Emitting Diode) control screen, a magnetic stirring unit, a pump unit, a pressure sensing unit, a temperature sensing unit, a membrane chamber, a sample can and a circuit board, wherein the LED control screen is located at the left of the shell; the sample can is located at the right of the shell; the shell at the bottom of the sample can is internally provided with the magnetic stirring unit; the pump unit is located in the shell, one end of the pump unit is connected with a sample outlet in the bottom of the sample can, and the other end of the pump unit is connected to the membrane chamber and reaches to a membrane bag; the membrane chamber is located at the rear of an instrument; relevant circuits of all the units are directly connected to the central circuit board; and all the units are controlled by using the LED control screen and the circuit board. The device has the advantages that the quantized monitoring for the ultrafiltration process is realized through the LED control screen; due to the design of the membrane chamber, the device can be compatible with various brands of membrane bags and is attractive in appearance and convenient; and meanwhile, the cost can be favorably reduced, and the application requirement of different crowds can be met to the maximum extent.
Description
Technical field
The utility model relates to a kind of biochemical separator; the tangential flow filtration that belongs to the molecular weight level; especially relate to a kind of employing membrane separation technique; usually molecular cut off is 1-1000KD, is widely used in the tangential flow ultra-filtration unit of bioengineering, bio-pharmaceuticals, chemistry, papermaking, Food Science, ocean engineering and field of environment protection.
Background technology
Ultrafiltration claims hyperfiltration again, is to be one of membrane separation technique of motive force with pressure, utilizes film surface mechanical grading, fenestra retardance and the adsorbing comprehensive effect of film surface fenestra, and reaching big molecule and little molecular separation is purpose.In ultra-filtration process, the aqueous solution film surface of flowing through under pressure promotes sees through rete less than solvent and the little molecule solute of fenestra, becomes cleaner liquid; Solute and the solute group bigger than fenestra are trapped, and discharge with solvent, become concentrate.Ultra-filtration process is dynamic filtration, separation process is finished under flow regime, have easy and simple to handle, with low cost, need not increase the experiment condition gentleness of advantage, the especially hyperfiltration technique of any chemical reagent, compare the variation that does not have phase with evaporation, freeze drying, and do not cause the variation of temperature, PH, thereby can prevent sex change, inactivation and the self-dissolving of large biological molecule.But ultrafiltration also has certain limitation, and it can not directly obtain dry powder formulations.For protein solution, generally can only obtain 10%～50% concentration.
Cross-flow ultrafiltration (Tangential flow ultrafiltration) refers to liquid flow direction and filtering direction ultrafiltration form in vertical direction.The flow direction of traditional vertical its liquid of filtration is consistent with filtering direction, along with the carrying out of filtering, cake layer or gel layer thickness that filter membrane surface forms increase gradually, and flow velocity reduces gradually, so the ability of the processing feed liquid of dead-end filtration is subjected to certain restriction.And cross-flow ultrafiltration produces shearing force on the filter medium surface in the liquid flow process, has reduced the accumulation of filter cake or gel layer, has guaranteed the stable rate of filtration, and this has important role concerning whole filter process.
The key of hyperfiltration technique is film, and film has various type and specification, can select for use according to requirements of one's work.Early stage film is isotropic uniform films, i.e. Chang Yong microporous membrane, and its aperture is 0.05mm and 0.025mm normally.In recent years, some anisotropic asymmetric milipore filters have appearred on the market, a kind of anisotropy diffusion barrier, porous " skin layer " with certain pore size extremely thin by one deck wherein, relative heavy back with one deck many easier to be logical that ooze, form as " spongy layer " that support usefulness.Skin layer has determined the selective of film, and spongy layer has increased mechanical strength.Because skin layer is extremely thin, therefore efficient, permeability is good, flow is big, and be difficult for being blocked by solute and cause flow velocity to descend.Film commonly used generally is to be made by the two mixture of acetate fiber or cellulose nitrate or this.For adapting to the needs of sterilizing in pharmacy and the food industry, developed the anisotropic membrane of non-fibrous type, for example PS membrane, polysulfonamides film and polyacrylonitrile film etc. in recent years.This film all is stable in PH1～14, and can be 90 ℃ of following operate as normal.
Ultrafiltration originated from 1748, but until the sixties in 20th century, proposition along with the molecular level concept, beginning of modern hyperfiltration technique development at last, the seventies and the eighties are the develop rapidly stages of hyperfiltration technique, external hyperfiltration technique just has been tending towards ripe after the nineties, and the diversification of market ultrafiltration commodity is widely used in every field.China is later to this technical research, still is in the research time limit seventies, the end of the eighties, just enter suitability for industrialized production and application stage, technical development at present is in good condition, and many product qualities are not second to external product, but product use still have many worth raisings aspect.
Cross-flow ultrafiltration equipment is mainly based on imported product in the market, the domestic product that has some producers that small-sized or easy cross-flow ultrafiltration are arranged.Film product as technological core has abundant product resource in market, but because developer's difference, model difference between the different film products, need different separately adapting appts, limited the idea that the user wants to carry out abundanter application to a certain extent, therefore, develop one's own moulding cross-flow ultrafiltration product and can accomplish that compatible domestic and international various different film products become a project that receives much concern in this field.
Summary of the invention
In order to overcome the existing domestic moulding cross-flow ultrafiltration equipment that still do not have, the situation of import equipment costliness, the utility model provides a kind of novel tangential flow ultra-filtration unit, this device is in conjunction with the present market demand to cross-flow ultrafiltration equipment, multiple cross-flow ultrafiltration functional part is become one, and with micro computer whole device is monitored, whole cross-flow ultrafiltration process is quantized, visual straight control, easy and simple to handle, the temperature and pressure sensor that carries provides the pressure and temperature data in real time, warning line can be set report to the police automatically, and is safe and reliable; Pipeline junctional membrane bag is directly used in the film chamber, can adaptive various film bag, and it is convenient to change, and maximizes compatible various experimental program.
The technical scheme that its technical problem that solves the utility model adopts is: a kind of tangential flow ultra-filtration unit comprises device case, the LED control flow, the magnetic agitation unit, pump unit, pressure sensitive unit, temperature sensing unit, film chamber, sample jar, circuit board is formed, and the LED control flow is positioned at the housing left side, and sample jar is positioned at the housing right side, be the magnetic agitation unit in the sample jar bottom shell, the pump unit is positioned at enclosure interior, and an end connects sample jar bottom outlet, and an end junctional membrane chamber arrives the film bag; The film chamber is positioned at the instrument rear, and each unit interlock circuit is directly connected on the circuit board of central authorities, by LED control flow and the common regulation and control that realize each unit of circuit board.
Described pump unit adopts the membrane pump of anti-deep-etching, and pump housing size is little, and it is non-maintaining not have oil, quiet microseismic activity during running, and auto-pumping can be done running, and running speed is controlled in real time by the LED control flow.
Described magnetic agitation unit is positioned at the housing of sample jar bottom, adopts slim motor, and running speed is controlled in real time by the LED control flow, evenly rotates by sample liquids in the magneticaction drive sample jar, reaches to make the stable purpose of each component of sample liquid.
Described film chamber directly connects pump, sample jar, receiving flask by pipeline, by opening the film bag that the side wicket can directly connect various brands, hydrostatic sensor and the temperature sensor installed at wall simultaneously can show pressure and temperature in real time, to ultrafiltration environment intuitive display.
The novel beneficial effect of this use is, it is attractive in appearance to adopt the structure of film chamber that instrument is not lost in can the film bag of compatible each brand both at home and abroad; Hydrostatic sensor on the film locular wall and temperature sensor guarantee that the instrument user can understand the physicochemical property of sample liquids in real time; Can carry out high-precision electronic control to pump unit and the magnetic agitation unit of instrument by the LED control flow, science is accurate more, can satisfy the experimental implementation that most needs carry out ultra-filtration process, the most frequently used have: the microorganism collection experiment, different molecular weight protein, the polyose separation process, small-molecule substance is except sugar, except albumen, except the thermal source operation, protein, polyose concentrates, desalination, dealcoholysis, exchange buffering liquid, dialysis waits experiment and pharmaceutical industry, monoclonal antibody, recombinant protein, vaccine, gene therapy medicament, the macromolecular purifying of blood product and other biological and concentrated.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Fig. 1 is basic pattern structures sketch of the present utility model.
Fig. 2 is the simple structure sketch of overlooking of the present utility model.
Fig. 3 is outward appearance sketch of the present utility model.
Wherein:
LED control flow 1
Liquid-pressure pick-up unit 4
Liquid-pressure pick-up and temperature sensor unit 5
Pump unit 6
Sample jar 7
Magnetic agitation unit 8
Receiving flask 10
Sample jar material-feeding port 11
Sample jar gas supplementing opening 12
The specific embodiment
The sample that need carry out ultrafiltration adds sample jar 7, each composition forms the comparatively state of stable homogeneous under the stirring of magnetic agitation unit 8, sample liquid is pumped under the effect of pump unit 6 in the film bag unit 2 in the film chamber 3, because the centrifugation of film, small-molecule substance sees through film with solvent and is collected bottle 10 collections, macromolecular substances then continues to become tangential sample liquid flow to return sample jar 7 with film, finishes whole ultra-filtration process.The speed of pump and the speed of magnetic agitation can be accomplished accurate control by LED control flow 1, and the liquid-pressure pick-up unit 4 on the 3 inwall pipelines of film chamber normally is presented at pressure and temperature on the LED control flow 1 with temperature sensor unit 5, and is convenient directly perceived.Material-feeding port 11 and the gas supplementing opening 12 on sample jar 7 tops can use according to experimenter's demand, satisfy diversified experiment demand.
Claims (4)
1. a tangential flow ultra-filtration unit comprises device case, the LED control flow, the magnetic agitation unit, pump unit, pressure sensitive unit, temperature sensing unit, film chamber, sample jar, circuit board is formed, and the LED control flow is positioned at the housing left side, and sample jar is positioned at the housing right side, be the magnetic agitation unit in the sample jar bottom shell, the pump unit is positioned at enclosure interior, and an end connects sample jar bottom outlet, and an end junctional membrane chamber arrives the film bag; The film chamber is positioned at the instrument rear, and each unit interlock circuit is directly connected on the circuit board of central authorities, by LED control flow and the common regulation and control that realize each unit of circuit board.
2. a kind of tangential flow ultra-filtration unit according to claim 1, it is characterized in that: described pump unit adopts the membrane pump of anti-deep-etching, and pump housing size is little, it is non-maintaining not have oil, quiet microseismic activity during running, auto-pumping, can do running, running speed is controlled in real time by the LED control flow.
3. a kind of tangential flow ultra-filtration unit according to claim 1, it is characterized in that: described magnetic agitation unit is positioned at the housing of sample jar bottom, adopt slim motor, running speed is controlled in real time by the LED control flow, drive by magneticaction that sample liquids evenly rotates in the sample jar, reach to make the stable purpose of each component of sample liquid.
4. a kind of tangential flow ultra-filtration unit according to claim 1, it is characterized in that: described film chamber directly connects pump, sample jar, receiving flask by pipeline, by opening the film bag that the side wicket can directly connect various brands, hydrostatic sensor and the temperature sensor installed at wall simultaneously can show pressure and temperature in real time, to ultrafiltration environment intuitive display.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320213578 CN203220855U (en) | 2013-04-22 | 2013-04-22 | Tangential flow ultrafiltration device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320213578 CN203220855U (en) | 2013-04-22 | 2013-04-22 | Tangential flow ultrafiltration device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203220855U true CN203220855U (en) | 2013-10-02 |
Family
ID=49247873
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320213578 Expired - Fee Related CN203220855U (en) | 2013-04-22 | 2013-04-22 | Tangential flow ultrafiltration device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203220855U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936889A (en) * | 2014-03-19 | 2014-07-23 | 苏州英诺凯生物医药科技有限公司 | Method for purification of enoxaparin by tangential flow filtration |
-
2013
- 2013-04-22 CN CN 201320213578 patent/CN203220855U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936889A (en) * | 2014-03-19 | 2014-07-23 | 苏州英诺凯生物医药科技有限公司 | Method for purification of enoxaparin by tangential flow filtration |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2092974A1 (en) | A method and apparatus for membrane separation applying concentration polarization, and an extrctor specially used thereof | |
| WO2000024499A1 (en) | A cross-flow filtration apparatus | |
| CN115060571A (en) | Liquid-to-liquid bio-particle concentrator with disposable fluid path | |
| CN103173933B (en) | A kind of preparation method of glucose vinyl acetate/N-isopropylacrylamide copolymer nano tunica fibrosa | |
| CN203220855U (en) | Tangential flow ultrafiltration device | |
| CN102899247B (en) | A blood cell separation device | |
| CN204637683U (en) | A kind of slipstream filter post purification devices of multicolumn parallel connection | |
| CN102083482A (en) | Device for filtering a complex liquid such as blood, particularly applicable to an autotransfuser | |
| CN103059159B (en) | Process for extracting mannan from beer yeast powder | |
| CN202415421U (en) | Simple dynamic protein dialysis system | |
| JP5696527B2 (en) | Plasma separation method using plasma separation membrane module | |
| CN204996339U (en) | Improved generation health level hollow fiber filtration membrane group spare | |
| CN116672533A (en) | Medical device | |
| CN106047963A (en) | Method for producing high fructose syrup by immobilized glucose isomerase | |
| CN205774238U (en) | A kind of remove impurity purification system of vitamin C glucoside | |
| CN208389793U (en) | A kind of blood plasma micro-filtration structure and its vortex tube structure | |
| CN204933266U (en) | A kind of lentinan grading purification filtration system | |
| CN211097902U (en) | Virus filtering, adsorbing and concentrating device | |
| Michaels | Fifteen years of ultrafiltration: problems and future promises of an adolescent technology | |
| CN209301846U (en) | A pressurized rapid dialysis device | |
| KR102708545B1 (en) | Separation device and method for separating target particles from a liquid sample | |
| CN221759848U (en) | A separation and preparation device | |
| CN208839808U (en) | Separator is used in a kind of HMG production | |
| CN203899463U (en) | Membrane filtration device | |
| CN218290874U (en) | Extracellular vesicle circulating separation and purification platform |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: Hangzhou Neoline Technology Co., Ltd. Document name: Notification to Pay the Fees |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: Hangzhou Neoline Technology Co., Ltd. Document name: Notification of Termination of Patent Right |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131002 Termination date: 20180422 |