CN203212573U - Device for improving culturability of microorganisms - Google Patents
Device for improving culturability of microorganisms Download PDFInfo
- Publication number
- CN203212573U CN203212573U CN 201320247504 CN201320247504U CN203212573U CN 203212573 U CN203212573 U CN 203212573U CN 201320247504 CN201320247504 CN 201320247504 CN 201320247504 U CN201320247504 U CN 201320247504U CN 203212573 U CN203212573 U CN 203212573U
- Authority
- CN
- China
- Prior art keywords
- culture dish
- water storage
- dish ware
- ware body
- microorganisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000012780 transparent material Substances 0.000 claims abstract description 5
- 239000011159 matrix material Substances 0.000 claims description 14
- 238000001914 filtration Methods 0.000 claims description 13
- 230000000295 complement effect Effects 0.000 claims description 3
- 239000012528 membrane Substances 0.000 abstract description 5
- 239000000758 substrate Substances 0.000 abstract 4
- 230000000813 microbial effect Effects 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000007747 plating Methods 0.000 description 2
- 230000011664 signaling Effects 0.000 description 2
- 208000035126 Facies Diseases 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a microorganism culture device, and provides a device for improving the culturability of microorganisms. The device comprises a culture dish cover 1, a culture dish body 2, a micropore filter membrane 3 and a water storage substrate 4. The water storage substrate 4 is placed at the inner bottom of the culture dish body 2, the shape and the size of the water storage substrate 4 are matched with the shape and the size of the inner bottom of the culture dish body 2, the micropore filter membrane 3 covers the upper surface of the water storage substrate 4, the culture dish cover 1 is made of transparent materials, the shape of the culture dish cover 1 is matched with that of the culture dish body 2, the inner diameter of the culture dish cover 1 is larger than the outer diameter of the culture dish body 2, the height of the culture dish cover 1 is smaller than that of the culture dish body 2, and the culture dish cover 1 is placed on the culture dish body 2. The device has the advantages that the culturability of the microorganisms can be strengthened, the planting efficiency of the microorganisms is improved, and even some microorganisms which cannot be cultured under conventional conditions can grow by the aid of the device.
Description
Technical field
The utility model relates to microbial cultivation device.
Background technology
Chinese patent application numbers 201220224703.9 discloses " microbial cultivation device ", comprise the stand of being made by transparent material and be located at culture tube in the stand, described stand is provided with at least two bracing frames, is provided with the through hole that passes for culture tube at bracing frame.The utility model can prevent effectively that substratum from being polluted, and is convenient to observe the culturing process of microorganism; Whole apparatus structure novelty, with low cost, its special construction have guaranteed the security of substratum in transportation.
Chinese patent application numbers 201120132508.9 discloses a kind of " a kind of microbial cultivation device ", comprise culture dish, it is characterized in that, also comprise support, described support comprises base, top cover and vertically connects base and at least two parallel poles of top cover, is placed with the culture dish of a plurality of stacks between described base and the top cover; Described culture dish comprises ware body and ware lid, described ware body comprises diapire and perpendicular to the ware body sidewall of diapire, described ware body sidewall is provided with at least two the grooves perpendicular to diapire corresponding with the pole position, described ware lid is transparent plate, described ware covers and is provided with at least two apertures corresponding with the pole position, and described pole is in corresponding groove and aperture.
Summary of the invention
The purpose of this utility model is to provide a kind of device that can effectively improve the raising microorganism cultivation property of microorganism plating efficiency.
A kind of device that improves microorganism cultivation property described in the utility model is made of culture dish ware lid, culture dish ware body, millipore filtration and water storage matrix, water storage matrix is placed on culture dish ware body inner bottom part, its shape and size and culture dish ware body inner bottom part are complementary, cover millipore filtration above, culture dish ware lid is transparent material, shape matches with culture dish ware body, the internal diameter of culture dish ware lid is greater than the external diameter of culture dish ware body, height is less than culture dish ware body, and culture dish ware lid is placed on the culture dish ware body.
Described millipore filtration aperture is 0.25-0.5um, and millipore filtration thickness is 100-200um.The thickness of described water storage matrix is 3-8mm.
Millipore filtration aperture 0.25-0.5um in the utility model, can play a supporting role to the upper strata solid medium that in culturing process, adds, can guarantee that also prokaryotic organism such as various ions, signaling molecule, moisture and bacterium see through in the solid medium that filter membrane arrives the upper strata from water storage matrix, make the microorganism that in substratum, grows to obtain culture environment like the former growth environmental facies.The water storage matrix of lower floor provides moisture and part nutritive substance for the upper strata substratum, the more important thing is make the microorganism that grows on the substratum can with former growth environment in biology and abiotic factor carry out information communication.Culture dish ware lid slightly larger in diameter highly is slightly less than the ware body in the ware body, cover the ware lid after, still have the little air circulation inside and outside the ware.
Device described in the utility model can strengthen the cultivation property of microorganism, improves the microorganism plating efficiency.Even some generally can not be grown by cultured microorganism.Be conducive to the separation of slow growth microorganism.Also can guarantee simultaneously for a long time culturing micro-organisms and be unlikely to make because substratum is dry microbial death.The utility model novel structure, with low cost, make simply, easy and simple to handle, low consumption environment protection.
Description of drawings
Fig. 1 is culture dish ware lid described in the utility model.
Fig. 2 is culture dish ware body described in the utility model.
Among the figure: 1-culture dish ware lid, 2-culture dish ware body, 3-millipore filtration, 4-water storage matrix.
Embodiment
Below in conjunction with accompanying drawing the utility model is further described, this description is not construed as limiting the utility model.
In the present embodiment, water storage matrix 4 is placed in the bottom in culture dish ware body 2, and the thickness of water storage matrix 4 is 3-8mm, and water storage matrix can be sponge.The sterilization back adds the liquid of simulation microorganism environment of living in water storage matrix, cover the aseptic millipore filtration 3 of one deck on the water storage matrix, and the aperture of millipore filtration is 0.25-0.5um, and the thickness of millipore filtration is 100-200um.
Add solid medium at millipore filtration.Moisture in the simulated environment liquid, ion or signaling molecule, even prokaryotic organism such as bacterium can arrive in the substratum on filter membrane upper strata by filter membrane, make the microorganism of media surface growth can obtain the culture environment similar to its growing environment.Culture dish ware lid matches with culture dish ware body for transparent material, and slightly larger in diameter highly is slightly less than culture dish ware body in culture dish ware body, during use ware is placed on the ware body, and forward is positioned in the suitable environment.
Claims (3)
1. device that improves microorganism cultivation property, it is characterized in that by culture dish ware lid (1), culture dish ware body (2), millipore filtration (3) and water storage matrix (4) constitute, water storage matrix (4) is placed on culture dish ware body (2) inner bottom part, its shape and size and culture dish ware body (2) inner bottom part are complementary, cover millipore filtration (3) above, culture dish ware lid (1) is transparent material, shape and culture dish ware body (2) are complementary, the internal diameter of culture dish ware lid (1) is greater than the external diameter of culture dish ware body (2), height is less than culture dish ware body (2), and culture dish ware lid (1) is placed on the culture dish ware body (2).
2. as claimed in claim 1, it is characterized in that in described millipore filtration (3) aperture be 0.25-0.5um, thickness is 100-200um.
3. as claimed in claim 1, it is characterized in that the thickness in described water storage matrix (4) is 3-8mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320247504 CN203212573U (en) | 2013-05-09 | 2013-05-09 | Device for improving culturability of microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320247504 CN203212573U (en) | 2013-05-09 | 2013-05-09 | Device for improving culturability of microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203212573U true CN203212573U (en) | 2013-09-25 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320247504 Expired - Fee Related CN203212573U (en) | 2013-05-09 | 2013-05-09 | Device for improving culturability of microorganisms |
Country Status (1)
| Country | Link |
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| CN (1) | CN203212573U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104371914A (en) * | 2014-11-18 | 2015-02-25 | 海门市优耐特实验器材发展有限公司 | Sampling vessel |
| CN104789449A (en) * | 2015-04-27 | 2015-07-22 | 国家海洋局第一海洋研究所 | In-situ culturing device for deep-sea microbes |
| CN105670914A (en) * | 2016-04-26 | 2016-06-15 | 湖南生物机电职业技术学院 | Petri dish capable of continuously supplementing impact factors |
| CN105695312A (en) * | 2016-04-26 | 2016-06-22 | 湖南生物机电职业技术学院 | Microorganism gradient plate culture device and method |
| CN111197000A (en) * | 2020-03-16 | 2020-05-26 | 华东理工大学 | High temperature resistant ammonia oxidizing bacteria culture device and method |
-
2013
- 2013-05-09 CN CN 201320247504 patent/CN203212573U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104371914A (en) * | 2014-11-18 | 2015-02-25 | 海门市优耐特实验器材发展有限公司 | Sampling vessel |
| CN104789449A (en) * | 2015-04-27 | 2015-07-22 | 国家海洋局第一海洋研究所 | In-situ culturing device for deep-sea microbes |
| CN105670914A (en) * | 2016-04-26 | 2016-06-15 | 湖南生物机电职业技术学院 | Petri dish capable of continuously supplementing impact factors |
| CN105695312A (en) * | 2016-04-26 | 2016-06-22 | 湖南生物机电职业技术学院 | Microorganism gradient plate culture device and method |
| CN105695312B (en) * | 2016-04-26 | 2017-12-08 | 湖南生物机电职业技术学院 | A kind of microorganism gradient plate culture apparatus and method |
| CN105670914B (en) * | 2016-04-26 | 2018-03-02 | 湖南生物机电职业技术学院 | It is a kind of can continuous supplementation factor of influence culture dish |
| CN111197000A (en) * | 2020-03-16 | 2020-05-26 | 华东理工大学 | High temperature resistant ammonia oxidizing bacteria culture device and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130925 Termination date: 20190509 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |