CN202482303U

CN202482303U - Culture medium preparing device

Info

Publication number: CN202482303U
Application number: CN 201220002805
Authority: CN
Inventors: 吴腾飞; 孙志明; 李庆海; 王建新
Original assignee: Shinva Medical Instrument Co Ltd
Current assignee: Shinva Medical Instrument Co Ltd
Priority date: 2012-01-06
Filing date: 2012-01-06
Publication date: 2012-10-10
Anticipated expiration: 2022-01-06

Abstract

The utility model provides a culture medium preparing device, belongs to the field of sterilization and disinfection equipment in medical apparatus and instruments, and in particular relates to a device for sterilizing and separately packaging culture medium. The culture medium preparing device comprises a reaction tank (1) and an exhaust system, an air inlet system, a water replenishing system, a water bath sterilizing device, a rapid cooling device and a feeding and sub-packaging system which are connected with the reaction tank (1), wherein the upper part of tank body of the reaction tank (1) is connected with the exhaust system through an air outlet (25), and is connected with the air inlet system through an air inlet (28); the middle part of the tank body is connected with the water replenishing system through a water replenishing hole (29), connected with the feeding system through a feeding hole and connected with the water bath sterilizing device through a water inlet (31); the other end of the water bath sterilizing device is connected with the rapid cooling device; and the other end of the rapid cooling device is connected with a water outlet (27) at the bottom of the reaction tank (1). The culture medium preparing device provided by the utility model can realize sterilization and sub-package, has the advantages of rapid cooling, high efficiency and the like, and effectively avoid secondary pollutions of the culture medium.

Description

The medium preparation device

Technical field

The medium preparation device belongs to the sterilization device field in the medicine equipment, is specifically related to the device of a kind of specialized application in medium sterilization, packing.

Background technology

Substratum is the nutriment that supplies mikrobe, plant, animal tissues and bacterial growth and keep the human configuration of growth usefulness; Be used for single plant microorganism culturing and evaluation fully after the sterilization; Generally all be under sterile state, to store, quantitatively use, belong to a kind of high technology industry.

The process of traditional preparation substratum is carried out human configuration with the substratum powder earlier, stirs, and then re-uses in the sterilization of steam sterilizer the inside inside installing to Erlenmeyer flask, and this needs the manpower and materials of labor, and efficient is not high.At present, the special culture media preparing device of external import can unite two into one configuration, sterilization, and last constant temperature maintenance etc. are to be packed.Greatly improved efficient, and saved manpower and materials, but external import equipment price comparison is expensive, therefore caused can not being in any more of production cost, on user's use range, caused certain limitation.

At present domestic do not have company to produce this special-purpose medium preparation device, and therefore, the completion substratum disposes, sterilization is integrated seems particularly important.

Summary of the invention

The technical problem that the utility model will solve is: overcome the deficiency of prior art, a kind of realize simultaneously configuration, sterilization are provided, the medium preparation device of finish back constant temperature packing, remove secondary pollution from, cooling is fast, production efficiency is high.

The utility model solves the technical scheme that its technical problem adopted: this medium preparation device; It is characterized in that: comprise retort and with the joining exhaust system of retort, inlet system, water charging system, water-bath sterilising plant, fast cooling device and the reinforced assembling system that divides; Retort tank body top connects exhaust system through venting port, connects inlet system through inlet mouth, and the tank body middle part connects water charging system through water supplement port; Connect charging system through charge cavity; Connect the water-bath sterilising plant through water-in, the water-bath sterilising plant the other end connects fast cooling device, the dewatering outlet of fast cooling device the other end coupled reaction pot bottom.Between water-bath sterilising plant, fast cooling device and tank body, form the recycle loop of water.

Described water-bath sterilising plant comprises the electrothermal tube of installation in vaporizer, the vaporizer and the vaporizer platinum resistance thermometer sensor, that vaporizer top is provided with, and the water inlet of vaporizer is arranged on the bottom, and water outlet is arranged on the top, the water-in of water outlet coupled reaction jar.Water gets into from base of evaporator, does not have behind the electrothermal tube to get into the tank body from vaporizer top water outlet, prevented the dry combustion method of electrothermal tube, and the profile of vaporizer is small and exquisite, has saved the internal duct space.

Described reinforced minute assembling system passes the discharge port of the charge cavity coupled reaction jar on the body skin through silicone tube one end that increases the weight of, and the silicone tube the other end extends the substratum bottom, and charge cavity is provided with a plurality of, respectively as the reinforced inlet and the outlet of packing.The silicone tube that increases the weight of extends the substratum bottom, guarantees to find time fully substratum, past reaction ladle addition when charge cavity also can be used for two-stage sterilization.

Described exhaust system is divided into two-way through two venting ports, and one the tunnel connects tensimeter and exhaust bolt in proper order, and another road connects exhaust solenoid valve and discharge filter in proper order.

Described inlet system comprises air inlet SV, air intake filter, sterile air strainer and the air that connects successively.Guarantee sterility, avoid secondary pollution.

Described water charging system comprises moisturizing SV, moisturizing strainer and the volume pump that connects successively, and volume pump one end is as water inlet.

Described fast cooling device comprises plate-type heat exchanger, in-line pump and advances the cold water SV, and the in-line pump output terminal is through the dewatering outlet of pipeline coupled reaction pot bottom, and the place, dewatering outlet also is provided with water drain ball valve.During cooling, advance the cold water valve opening solenoid, be cooled to 80 ℃ from 121 ℃ and only need about 10 minutes, can fast cooling, guarantee the quality of substratum and saved cooling time.

Described plate-type heat exchanger is provided with four interfaces; An interface connects the water inlet of base of evaporator; The inlet of a duct coupling pump, two other interface connect recirculated water discharge pipe line and circulating water inlet road respectively, and the circulating water inlet road is provided with into cold water SV.

Described retort is double-layer nested formula structure; Inside is provided with interlayer; Be provided with the interlayer platinum resistance thermometer sensor, in the interlayer, the outer bottom of tank body is provided with support, and the tank base in support is installed magnetic stirring apparatus; Outer ring, tank body top is provided with water-level float and vernier switch, and the tank body top also is provided with inner core platinum resistance thermometer sensor,, pressure unit and SV.

The motor magnet of described magnetic stirring apparatus is installed in retort tank body outer bottom, and the stirring rotator of magnetic stirring apparatus is installed in the tank body inner bottom part, rotates through the electric machine control stirrer.During the equipment operation, the rotation of control stirrer makes substratum be in the homogeneity of mixing state and assurance substratum temperature all the time.

Compared with prior art, the beneficial effect that the utility model had is:

1, realizes sterilization and packing simultaneously; Carry out the heating in water bath sterilization through vaporizer; And keep sterilising temp and sterilization time, the silicone tube that increases the weight of directly extends the substratum bottom, guarantees to find time completely substratum; When also can be used for two-stage sterilization, charge cavity, further removed the danger of secondary pollution from toward the interior secondary charging of retort;

2, prevent dry combustion method, saving space: adopt special vaporizer; Water gets into from the base of evaporator water inlet; Water level did not have that the water outlet from the vaporizer top gets into the tank body of retort behind the electrothermal tube; Effectively prevented the dry combustion method of electrothermal tube, and the profile of this vaporizer is small and exquisite, pipeline installing space easy for installation, as to have saved enclosure interior;

3, cooling is quick, temperature is even: during cooling, electrothermal tube is closed, and advances the cold water SV and opens; The results showed, be cooled to 80 ℃, a need about 10 minutes from 121 ℃; Can fast cooling, on the basis of the quality that has guaranteed substratum, saved a large amount of cooling times; When equipment moved, the electric machine control stirrer rotated synchronously, made substratum be in the mixing state all the time, and strong assurance the homogeneity of substratum temperature, further guarantee the quality of substratum.

Description of drawings

Fig. 1 is the utility model structural front view synoptic diagram.

Fig. 2 is the heating curve diagram intention of the utility model embodiment 1.

Fig. 3 is the heating curve diagram intention of the utility model embodiment 2.

Fig. 4 is the utility model housing surface structure front view synoptic diagram.

Wherein: 1; Retort 2; Air inlet SV 3; Air intake filter 4; Interlayer platinum resistance thermometer sensor, 5; Moisturizing SV 6; Moisturizing strainer 7; Sterile air strainer 8; Exhaust solenoid valve 9; Discharge filter 10; Air 11; Volume pump 12; Exhaust bolt 13; Water drain ball valve 14; Magnetic stirring apparatus 15; In-line pump 16; Advance cold water SV 17; Plate-type heat exchanger 18; Electrothermal tube 19; Vaporizer platinum resistance thermometer sensor, 20; Water-level float 21; Inner core platinum resistance thermometer sensor, 22; Pressure unit 23; SV 24; Tensimeter 25; Venting port 26; Vernier switch 27; Dewatering outlet 28; Inlet mouth 29; Water supplement port 30; Vaporizer 31; Water-in 32; Support 33; Interlayer.

Fig. 1～2nd, the most preferred embodiment of the utility model further specifies below in conjunction with 1～4 pair of the utility model of accompanying drawing:

Embodiment

Embodiment 1

With reference to accompanying drawing 1,2 and 4:

The medium preparation device; Comprise retort 1 and with retort 1 joining exhaust system, inlet system, water charging system, water-bath sterilising plant, fast cooling device and the reinforced assembling system that divides, retort 1 tank body top connects exhaust system through venting port 25, connects inlet systems through inlet mouth 28; The tank body middle part connects water charging system through water supplement port 29; Connect charging system through charge cavity, connect the water-bath sterilising plant through water-in 31, the water-bath sterilising plant the other end connects fast cooling device; The dewatering outlet 27 of fast cooling device the other end coupled reaction jar 1 bottom, the recycle loop of formation water between water-bath sterilising plant, fast cooling device and tank body.

The reinforced assembling system that divides connects charge cavity through silicone tube one end that increases the weight of, and the silicone tube the other end extends the substratum bottom, and charge cavity is provided with a plurality of, respectively as the inlet of reinforced usefulness and the outlet that packing is used.

The water-bath sterilising plant comprises the electrothermal tube 18 of installation in vaporizer 30, the vaporizer 30 and the vaporizer platinum resistance thermometer sensor, 19 that vaporizer 30 tops are provided with; Vaporizer 30 is provided with two; Be in parallel installed with; The water inlet of each vaporizer 30 all is arranged on the bottom, and water outlet all is arranged on top, the water-in 31 that water outlet coupled reaction jar 1 middle part is provided with.

Exhaust system is divided into two-way through two venting ports 25, and one the tunnel connects tensimeter 24 and exhaust bolt 12 in proper order, and another road connects exhaust solenoid valve 8 and discharge filter 9 in proper order.

Inlet system comprises air inlet SV 2, air intake filter 3, sterile air strainer 7 and the air 10 that connects successively, and air inlet SV 2 one ends connect inlet mouth 28.

Water charging system comprises moisturizing SV 5, moisturizing strainer 6 and the volume pump 11 that connects successively, and volume pump 11 1 ends are as water inlet, and moisturizing SV 5 one ends connect water supplement port 29.

Fast cooling device comprises plate-type heat exchanger 17, in-line pump 15 and advances cold water SV 16, the dewatering outlet 27 that in-line pump 15 output terminals are provided with through pipeline coupled reaction jar 1 bottom, and 27 places, dewatering outlet also are provided with water drain ball valve 13.Plate-type heat exchanger 17 is provided with four interfaces; An interface connects the water inlet of vaporizer 30 bottoms; The inlet of a duct coupling pump 15, two other interface connect recirculated water discharge pipe line and circulating water inlet road respectively, and the circulating water inlet road is provided with into cold water SV 16.

Retort 1 is double-layer nested formula structure, and inside is provided with interlayer 33, is provided with interlayer platinum resistance thermometer sensor, 4 in the interlayer 33; The outer bottom of tank body is provided with support 32; Tank base in support 32 is equipped with magnetic stirring apparatus 14, and outer ring, tank body top is provided with water-level float 20 and vernier switch 26, and the tank body top also is provided with inner core platinum resistance thermometer sensor, 21, pressure unit 22 and SV 23; Tank interior also is provided with interlayer, is provided with interlayer platinum resistance thermometer sensor, 4 in the interlayer.The motor magnet of magnetic stirring apparatus 14 is installed in retort 1 tank body outer bottom, and the stirring rotator of magnetic stirring apparatus 14 is installed in the tank body inner bottom part, rotates through the electric machine control stirrer.

Working process is following:

Be used for the preparation standard substratum

Making processes: at first in tank body interlayer 33, inject an amount of water to pass through water charging system by operator, and through volume pump 11 countings, probably about 9L.Turn on the power switch, behind the wait 15s, function screen shows key frame, the making of choice criteria substratum.

In retort 1, inject through the sterile air after filtering through inlet system then, the water inlet from vaporizer 30 bottoms adds water in vaporizer 30 then, treats that water level did not have after the electrothermal tube 18; Water-bath is got in the retort 1 by the water outlet at vaporizer 30 tops, and electrothermal tube 18 work continue to heat; When temperature does not also reach preset temperature, then get into the temperature rise period, magnetic stirring apparatus 14 begins to stir simultaneously; In-line pump 15 is started working, and the water of the urceolus of retort 1 begins the circulation heating, up to reaching preset sterilising temp; If the water level of urceolus is low excessively, small pump can be through the moisturizing in the interlayer 33 of retort 1 of water charging system discontinuity; If the hypotony in the interlayer 33 at this moment, topping-up pump can be worked, and makes the pressure in the interlayer 33 be higher than certain value, guarantees that liquid is unlikely to boiling or splashes.

When passing through vaporizer 30, during as 121 ℃, keep this temperature with the persistently overheating sterilising temp of temperature to preliminary election; Retort 1 inner room fluid temperature then changes sterilization phase over to after reaching preset sterilising temp, and maintains sterilising temp and arrive up to sterilization time; Being used for the preliminary election sterilization holds time; Meanwhile, through the stirrer rotation of electric machine control magnetic stirring apparatus 14, make substratum be in the homogeneity of mixing state and assurance substratum temperature all the time.

Keep 121 ℃, and after lasting 20 minutes, the high temperature bath in the retort 1 is interior by in the in-line pump 15 entering plate-type heat exchangers 17 through the in-line pump 15 of the dewatering outlet 27 entering fast cooling devices of bottom; At this moment, electrothermal tube 18 is closed, and advances cold water SV 16 and opens; High temperature bath is discharged from the recirculated water discharge pipe line of plate-type heat exchanger 17, and cold water is got in the plate-type heat exchanger 17 by the circulating water inlet road through advancing cold water SV 16 again, is got in the vaporizer 30 by plate-type heat exchanger 17 again; And then import in the retort through vaporizer 30, substratum can be cooled fast to the outlet temperature of preliminary election at short notice, as 45 ℃; Behind the cooling temperature that the temperature of retort 1 tank body inner core is cooled to preset; Then get into holding stage, holding stage is to continue always, and sustainable this temperature of keeping is until stop.Standard medium can complete, and then through charge cavity and the output of the silicone tube that increases the weight of, stretches into the bottom of substratum by silicone tube one end, with this substratum is found time fully, avoids forming secondary pollution.Also can the gas in the retort 1 be discharged through exhaust system.

Embodiment 2

With reference to accompanying drawing 3:

Be used to prepare chocolate agar medium

Production process: at first also be to get into the temperature rise period, temperature risen to the sterilising temp of preliminary election, as 121 ℃; Keep for some time (general 15min), be cooled fast to the charge temperature of preliminary election subsequently, as 60 ℃; Carry out secondary charging through charge cavity then; Click the button of sterilizing for the second time after reinforced, program gets into sterilization for the second time, promptly begins sterilization cycle for the second time; Once more temperature is risen to second preselected temperature, as 100 ℃, and lasting for some time (being generally 15min); Cooling once more; Be quickly cooled to the outlet temperature of preliminary election,, and keep this final preselected temperature as 45 ℃; Until closing of program, the working process of each system of the medium preparation device of the utility model is identical with embodiment 1 with principle of work.

Embodiment 3

Be used for the preparation of complicated substratum

Be at first to heat up equally, arrive the sterilising temp that realization configures, keep this temperature for some time then, i.e. sterilization is held time; Be quickly cooled to the sterilising temp second time of preliminary election, and release pressure, operator can select to feed in raw material in this stage, add material afterwards in this temperature maintenance for some time; Carry out the second time of cooling fast again, be cooled to preselected temperature three, operator can select to feed in raw material, and keep for some time after feeding in raw material, and are cooled to the outlet temperature of preliminary election then, and insulation, keep this temperature, until closing of program.

Complicated substratum has sterilization process three times, is to accomplish automatically, as long as set the temperature and time of three sterilizations before.The working process of each system is identical with embodiment 1 with principle of work.

Embodiment 4

Be used for medium sterilization

This process is identical with the sterilization process of embodiment 1, heats up earlier, behind the arrival preliminary election sterilising temp, continues insulation, is quickly cooled to the outlet temperature of preliminary election then, and keeps outlet temperature until stop.

Embodiment 5

Be used for the substratum constant temperature water bath

The constant temperature water bath program has only holding stage.

Be about to temperature and rise to after the holding temperature of preliminary election, equipment maintains this holding temperature, substratum is continued insulation, until stop.

In addition, in sterilization process, the fluid temperature of retort inner room then changes sterilization phase over to after reaching preset sterilising temp; And maintaining sterilising temp arrives up to sterilization time; This step cycle water pump, magnetic stirring apparatus and topping-up pump are work; Electrothermal tube maintenance work, persistently overheating reaches the sterilization design temperature up to temperature.

The above; It only is the preferred embodiment of the utility model; Be not to be the restriction of the utility model being made other form, any professional and technical personnel of being familiar with possibly utilize the technology contents of above-mentioned announcement to change or be modified as the equivalent embodiment of equivalent variations.But every the utility model technical scheme content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment did, still belongs to the protection domain of the utility model technical scheme according to the technical spirit of the utility model.

Claims

1. medium preparation device; It is characterized in that: comprise be installed in the intravital retort of external shell (1) and with the joining exhaust system of retort (1), inlet system, water charging system, water-bath sterilising plant, fast cooling device and the reinforced assembling system that divides; Retort (1) tank body top connects exhaust system through venting port (25); Connect inlet system through inlet mouth (28); The tank body middle part connects water charging system through water supplement port (29), connects reinforced packing system through charge cavity, connects the water-bath sterilising plant through water-in (31); The water-bath sterilising plant the other end connects fast cooling device, the dewatering outlet (27) of fast cooling device the other end coupled reaction jar (1) bottom.

2. medium preparation device according to claim 1; It is characterized in that: described water-bath sterilising plant comprises the electrothermal tube (18) of installation in vaporizer (30), the vaporizer (30) and the vaporizer platinum resistance thermometer sensor, (19) that vaporizer (30) top is provided with; The water inlet of vaporizer (30) is arranged on the bottom; Water outlet is arranged on top, the water-in (31) of water outlet coupled reaction jar (1).

3. medium preparation device according to claim 1; It is characterized in that: described reinforced minute assembling system passes the discharge port of the charge cavity coupled reaction jar on the body skin through silicone tube one end that increases the weight of; The silicone tube the other end extends the substratum bottom; Charge cavity is provided with a plurality of, respectively as the reinforced inlet and the outlet of packing.

4. medium preparation device according to claim 1; It is characterized in that: described exhaust system is divided into two-way through two venting ports (25); One the tunnel connects tensimeter (24) and exhaust bolt (12) in proper order, and another road connects exhaust solenoid valve (8) and discharge filter (9) in proper order.

5. medium preparation device according to claim 1 is characterized in that: described inlet system comprises air inlet SV (2), air intake filter (3), sterile air strainer (7) and the air (10) that connects successively.

6. medium preparation device according to claim 1 is characterized in that: described water charging system comprises moisturizing SV (5), moisturizing strainer (6) and the volume pump (11) that connects successively, and volume pump (11) one ends are as water inlet.

7. medium preparation device according to claim 1; It is characterized in that: described fast cooling device comprises plate-type heat exchanger (17), in-line pump (15) and advances cold water SV (16); In-line pump (15) output terminal is through the dewatering outlet (27) of pipeline coupled reaction jar (1) bottom, and water drain ball valve (13) is located also to be provided with in dewatering outlet (27).

8. medium preparation device according to claim 7; It is characterized in that: described plate-type heat exchanger (17) is provided with four interfaces; An interface connects the water inlet of vaporizer (30) bottom; The inlet of a duct coupling pump (15), two other interface connect recirculated water discharge pipe line and circulating water inlet road respectively, and the circulating water inlet road is provided with into cold water SV (16).

9. medium preparation device according to claim 1; It is characterized in that: described retort (1) is double-layer nested formula structure; Inside is provided with interlayer (33); Be provided with interlayer platinum resistance thermometer sensor, (4) in the interlayer (33), the outer bottom of tank body is provided with support (32), and the tank base in support (32) is installed magnetic stirring apparatus (14); Outer ring, tank body top is provided with water-level float (20) and vernier switch (26), and the tank body top also is provided with inner core platinum resistance thermometer sensor, (21), pressure unit (22) and SV (23).

10. medium preparation device according to claim 9; It is characterized in that: the motor magnet of described magnetic stirring apparatus (14) is installed in retort (1) tank body outer bottom; The stirring rotator of magnetic stirring apparatus (14) is installed in the tank body inner bottom part, rotates through the electric machine control stirrer.