CN202157074U - Electrode blood culture bottle - Google Patents
Electrode blood culture bottle Download PDFInfo
- Publication number
- CN202157074U CN202157074U CN 201120250854 CN201120250854U CN202157074U CN 202157074 U CN202157074 U CN 202157074U CN 201120250854 CN201120250854 CN 201120250854 CN 201120250854 U CN201120250854 U CN 201120250854U CN 202157074 U CN202157074 U CN 202157074U
- Authority
- CN
- China
- Prior art keywords
- bottle
- culture
- container
- electrode
- blood culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000009640 blood culture Methods 0.000 title claims abstract description 13
- 230000012010 growth Effects 0.000 claims abstract description 11
- 244000005700 microbiome Species 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 239000004033 plastic Substances 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 2
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 5
- 239000008280 blood Substances 0.000 abstract description 5
- 210000004369 blood Anatomy 0.000 abstract description 5
- 238000012360 testing method Methods 0.000 abstract description 5
- 239000000758 substrate Substances 0.000 abstract description 3
- 210000001124 body fluid Anatomy 0.000 abstract description 2
- 239000010839 body fluid Substances 0.000 abstract description 2
- 208000031888 Mycoses Diseases 0.000 abstract 1
- 238000012136 culture method Methods 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 238000012258 culturing Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 239000003463 adsorbent Substances 0.000 description 4
- 235000011089 carbon dioxide Nutrition 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 229920005549 butyl rubber Polymers 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical class CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229940062057 nitrogen 80 % Drugs 0.000 description 1
- 235000021062 nutrient metabolism Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229940063666 oxygen 90 % Drugs 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides an electrode blood culture bottle, particularly providing a medical detecting device that is used for determining whether microorganisms exist in the blood and other aseptic body fluid of suspected patients with bacteria or fungus infection. The electrode blood culture bottle comprises a bottle body, a bottle bottom, a filter plate, a bottle cover and a rubber plug, after a filter plate is arranged between the bottle body and the bottle bottom, the bottle body can be screwed down; after being closed and sealed by the rubber plug and the bottle cover, the culture bottle becomes a sealed culture container; two electrodes are adopted as the bottom of the sealing culture container; the container is provided with various culture substrates suitable for the growth of the microorganisms at the middle part; and mixed gas is arranged in the container and above the culture substrates. The electrode blood culture bottle has a simple structure and can be used conveniently; the instrument test and manpower eyeballing are integrated into a whole so that the growth situation of the microorganisms can be clearly observed outside the bottle during the microorganism culture; furthermore, the continuous determination can be realized by instruments, so that the automatic determination can be realized by a traditional manual culture method, the sensitivity is high, the culture time is shortened, and the false negative results do not exist.
Description
Technical field
The medical science that relates to the utility model detects and uses device, is specially a kind of electroded Blood culture bottle.
Background technology
In the Clinical microorganism testing laboratory, hemoculture is one of a kind of most important and modal test.Instrument and the supporting Blood culture bottle that is used at present blood cultivation clinically is mainly import; The Bactectm9000 series of U.S. company BD and the Bact/Alert3D of French Mei Liai are arranged, and they adopt the method for fluorescence or color sensing to observe the CO that produces in the bacteria growth process
2Variable quantity judged whether bacteria growing, though this method positive is more accurate, false-negative appearance is arranged, for producing trace amounts of CO
2Bacterial strain have the phenomenon of omission, it is the instrument Blood culture bottle in addition, is inappropriate for visual observation, price is more expensive, is difficult for popularizing.
The utility model content
The technical problem that the utility model solved is to provide a kind of electrode Blood culture bottle, to solve the shortcoming in the above-mentioned background technology.
The technical problem that the utility model solved adopts following technical scheme to realize:
The electrode Blood culture bottle; Comprise bottle, the bottle end, screen plate, bottle cap and plug; Bottle can be tightened after letting slip filter plate with bottle in the middle of the end, and covering behind the plug seal bottle cap is a sealing culture vessel, and the sealing bottom of culture vessel is two electrodes; For being suitable for the various substratum of microorganism growth, internal tank, mixed gas is arranged above the substratum in the middle of the container.
Said bottle material is glass or plastics; The bottle end is that injection molded is processed, and has two electrodes, and electrode is stainless steel or silver; Screen plate is double-layer porous plastic plate, and the centre accompanies filtering membrane; Bottle cap and plug are the 18-20 type bottle cap and the freezing butyl rubber plug of medical standard.
Said mixed gas is carbonic acid gas, hydrogen and nitrogen mixture body, and its ratio is carbonic acid gas 10%, hydrogen 10%, nitrogen 80%, perhaps is carbonic acid gas and oxygen mixed gas, carbonic acid gas 10%, oxygen 90%.
Said filtering membrane is the plastic film of aperture 5-8 μ m.
Substratum in the said culturing bottle is limpid transparent liquid, can contain polymeric adsorbent.
The utility model is used for measuring the blood of doubting to bacterium or fungi infestation patient and whether has mikrobe with other aseptic body fluid, for mikrobe common in the blood provides appropriate nutrition composition and growing environment.Whether after the culturing bottle of inoculation sample is put into instrument, cultivate and monitoring continuously, measuring has bacterium and fungi growth.
Screen plate in the utility model is immersed in the substratum, and anticoagulant substances is arranged in the substratum, and polymeric adsorbent is on screen plate top; After adding blood; Red corpuscle is because the effect of screen plate can not get into the bottle bottom, if there is mikrobe in the test specimen, in culturing process; Can freely get into bottom the substratum owing to shake; At this moment the metabolism of mikrobe can make electric inert macromolecule substrate in the substratum, is electroactive small molecules product like nutrient metabolisms such as glucide, protein, fat, like amino acid, lactic acid salt, acetate etc.Along with microbial growth and breeding, the electric inert molecule in the substratum is replaced by electroactive substance gradually, thereby the electroconductibility of substratum is increased, and electricimpedance reduces, and the substratum of screen plate bottom becomes muddy simultaneously.The starting quantity of mikrobe is different, and the time that the Exponential growth phase occurs is also different, through setting up relation between the two, detects the electrical property change of substratum and extrapolates microbial growth.Microbial growth is judged in the variation of turbidity that also can be through visual inspection substratum bottom.
Beneficial effect: the utility model is simple in structure, and is easy to use, and instrument test and artificial visually examine are united two into one; Can be outside bottle when carrying out microorganism culturing clear observation microorganism growth situation; Also can realize METHOD FOR CONTINUOUS DETERMINATION, make the traditional hand cultural method realize measuring automatically through instrument, highly sensitive; Shortened incubation time, no false negative result.
Description of drawings
Fig. 1 is the structural representation of the utility model preferred embodiment.
Among the figure: bottle 1, the bottle end 2, screen plate 3, polymeric adsorbent 4, substratum 5, mixed gas 6, plug 7, bottle cap 8.
Embodiment
For technique means, creation characteristic that the utility model is realized, reach purpose and be easy to understand understanding with effect, below in conjunction with concrete diagram, further set forth the utility model.
Electrode Blood culture bottle referring to Fig. 1; Comprise bottle 1, the bottle end 2, screen plate 3, bottle cap 8 and plug 7; Can tighten after letting slip filter plate 3 at bottle 1 and the bottle end 2, covering behind plug 7 seal bottle caps 8 is a sealing culture vessel, and the sealing bottom of culture vessel is two electrodes; For being suitable for the various substratum 5 of microorganism growth, internal tank, mixed gas 6 is arranged above the substratum 5 in the middle of the container.
When the utility model uses, after putting into screen plate 3 at bottle 1 and the bottle end 2, tighten, from bottleneck pack into polymeric adsorbent 4 and liquid bacterial substratum 5; Vacuumize the back and inject mixed gas 6, internal layer is airtight with butyl rubber bung 7, again with bottle cap 8 sealing culturing bottles; Gas in the adjustment bottle forms the 6-10ml negative pressure, seals and covers 7; Through 115 ℃, 10 pounds autoclavings 20 minutes, take out, labelledly then become.
More than show and described the advantage of ultimate principle, principal character and the utility model of the utility model.The technician of the industry should understand; The utility model is not restricted to the described embodiments; The principle of describing in the foregoing description and the specification sheets that the utility model just is described; Under the prerequisite that does not break away from the utility model spirit and scope, the utility model also has various changes and modifications, and these variations and improvement all fall in the utility model scope that requires protection.The utility model requires protection domain to be defined by appending claims and equivalent thereof.
Claims (2)
1. electrode Blood culture bottle; It is characterized in that, comprise bottle, the bottle end, screen plate, bottle cap and plug, bottle can be tightened after letting slip filter plate with bottle at the end; Covering behind the plug seal bottle cap is a sealing culture vessel; The sealing bottom of culture vessel is two electrodes, for being suitable for the various substratum of microorganism growth, internal tank, mixed gas is arranged above the substratum in the middle of the container.
2. electrode Blood culture bottle according to claim 1 is characterized in that, said bottle material is glass or plastics; Bottle is that injection molded is processed the end, on have electrode; Screen plate is double-layer porous plastic plate, and the centre accompanies filtering membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120250854 CN202157074U (en) | 2011-07-16 | 2011-07-16 | Electrode blood culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120250854 CN202157074U (en) | 2011-07-16 | 2011-07-16 | Electrode blood culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202157074U true CN202157074U (en) | 2012-03-07 |
Family
ID=45764716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120250854 Expired - Fee Related CN202157074U (en) | 2011-07-16 | 2011-07-16 | Electrode blood culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202157074U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673664A (en) * | 2015-02-15 | 2015-06-03 | 武汉迪艾斯科技有限公司 | Blood sample culture device |
| CN115537312A (en) * | 2022-10-12 | 2022-12-30 | 宁波天康生物科技有限公司 | Magnetic blood culture bottle and online electrical impedance detection blood culture instrument composed of same |
-
2011
- 2011-07-16 CN CN 201120250854 patent/CN202157074U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673664A (en) * | 2015-02-15 | 2015-06-03 | 武汉迪艾斯科技有限公司 | Blood sample culture device |
| CN115537312A (en) * | 2022-10-12 | 2022-12-30 | 宁波天康生物科技有限公司 | Magnetic blood culture bottle and online electrical impedance detection blood culture instrument composed of same |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120307 Termination date: 20160716 |