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CN201825948U - Combined centrifuge tube for preparing cell blocks by using cast-off cells - Google Patents

Combined centrifuge tube for preparing cell blocks by using cast-off cells Download PDF

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Publication number
CN201825948U
CN201825948U CN2010205537829U CN201020553782U CN201825948U CN 201825948 U CN201825948 U CN 201825948U CN 2010205537829 U CN2010205537829 U CN 2010205537829U CN 201020553782 U CN201020553782 U CN 201020553782U CN 201825948 U CN201825948 U CN 201825948U
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China
Prior art keywords
filtration membrane
cell lump
tube
push pipe
collecting ring
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CN2010205537829U
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Chinese (zh)
Inventor
何洪敏
宋继兰
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Individual
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Individual
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Priority to CN2010205537829U priority Critical patent/CN201825948U/en
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Publication of CN201825948U publication Critical patent/CN201825948U/en
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Abstract

The utility model discloses a combined centrifuge tube for preparing cell blocks by using cast-off cells. The combined centrifuge tube comprises a top tube and is characterized in that the upper end and the lower end of the top tube are opened; a thread or a clamping groove is arranged at the lower part of the top tube; the lower part of the top tube is connected with a cell block collection ring; the upper end and the lower end of the cell block collection ring are opened; the lower part of the cell block collection ring is connected with a filtration membrane; the top tube is connected with a bottom tube through the threads or the clamping groove; the upper end of the bottom tube is opened and the lower end is closed; and the cell block collection ring and the filtration membrane are positioned in the top tube and the bottom tube. The combined centrifuge tube is simple in operation, directly realizes solid-liquid separation and enables the solid-phase sediment to have a certain normative shape simultaneously, and can be convenient for taking out complete cell blocks, thus being convenient for the subsequent operation.

Description

Cast-off cells prepare cell lump wax stone combined centrifugal pipe
Technical field
The utility model relates to and a kind ofly is used to make the combined centrifugal pipe that the cell lump wax stone uses for medical speciality is used to separate the preparation cast-off cells.
Background technology
The exfoliative cytology inspection is the important component part of medical pathologies and medical test, the general smear of variety of way that adopts is as the main means of checking, in real work, many positives, false positive and boundary property smear, need the patient further to draw materials or check, and the previous exfoliative cytology sample of getting is except that repeating to make the smear, owing to lack the means of sophisticated making cytology wax stone, generally all discarded, the utility model provides a kind of combined centrifugal pipe that the preparation cast-off cells are used to make the cell lump wax stone that separates, can be with the above-mentioned smear that need further draw materials or check, utilize original exfoliative cytology sample, be made as cytology wax stone, further verify and/or clarify a diagnosis, improve correct diagnosis with certain canonical schema.
At present, the used centrifuge tube of medical institutions is generally a monoblock type centrifuge tube, utilize whizzer to make that solid matter is deposited into the purpose that the pipe end reaches solid-liquid separation in the suspension, but still there is directly contact in the solid matter that is deposited on the pipe end with liquid phase substance, it can't kept the certain volume shape and is not having complete taking-up under the situation of unnecessary liquid phase composition as far as possible.
Summary of the invention
The purpose of this utility model provides a kind of cast-off cells and prepares cell lump wax stone combined centrifugal pipe, solve in the actual mechanical process, the solid matter piece that centrifugal back cell deposition thing forms can not accomplish from pipe that solid-liquid separates separately, the problem that the cell lump settling can't take out separately, make the solid precipitation thing in pipe, can accomplish solid-liquid separation, give the shape that the solid precipitation thing has certain standardization simultaneously, can take out easily, enter next step operation.
For achieving the above object, the technical solution adopted in the utility model is:
A kind of cast-off cells prepare cell lump wax stone combined centrifugal pipe, comprise push pipe, it is characterized in that: the upper and lower ends opening of push pipe, and the bottom of push pipe is threaded or draw-in groove; The bottom of push pipe is connected with the cell lump collecting ring, the upper and lower ends opening of cell lump collecting ring, and the bottom of cell lump collecting ring is connected with filtration membrane; Push pipe is connected with bottom tube by screw thread or draw-in groove, the upper end open of bottom tube, lower end closed; Described cell lump collecting ring and filtration membrane are positioned at push pipe and bottom tube.
Prepare cell lump wax stone combined centrifugal pipe according to described cast-off cells, it is characterized in that: be connected with the filtration membrane supporting ring below the described filtration membrane.
Prepare cell lump wax stone combined centrifugal pipe according to described cast-off cells, it is characterized in that: be threaded or draw-in groove on the outer wall of described push pipe bottom, correspondence is threaded or draw-in groove on the inwall of described bottom tube, on the tube wall of the screw thread of bottom tube or draw-in groove below annular platform is arranged, place described cell lump collecting ring, filtration membrane, filtration membrane supporting ring on the annular platform.
Prepare cell lump wax stone combined centrifugal pipe according to described cast-off cells, it is characterized in that: described cell lump collecting ring top is bulge loop, the bottom is a seat, the inwall socket connection of the outer wall of bulge loop and push pipe bottom, and the outer wall of cell lump collecting ring lower seat is provided with anti-skidding line.
Advantage of the present utility model is: simple to operate, directly accomplish solid-liquid separation and give the shape that the solid precipitation thing has certain standardization simultaneously that cell lump is easy to take out, and is convenient to next step operation.
Description of drawings
Fig. 1 is a decomposition texture synoptic diagram of the present utility model.
Fig. 2 is a decomposition texture sectional view of the present utility model.
Fig. 3 is the structural representation after the utility model assembling.
In the accompanying drawing: 1, push pipe; 2, cell lump collecting ring; 3, filtration membrane; 4, bottom tube; 5, filtration membrane supporting ring.
Embodiment
The utility model is described in further detail below in conjunction with the drawings and specific embodiments:
The utility model, cast-off cells prepare the cell lump wax stone with combined centrifugal pipe such as Fig. 1, Fig. 2, shown in Figure 3, comprise push pipe 1, the upper and lower ends opening of push pipe 1, be threaded or draw-in groove on the outer wall of push pipe 1 bottom, correspondence is threaded or draw-in groove on the inwall of bottom tube 4; Push pipe 1 is connected the upper end open of bottom tube 4, lower end closed by screw thread or draw-in groove with bottom tube 4.
The bottom of push pipe 1 is connected with cell lump collecting ring 2, the upper and lower ends opening of cell lump collecting ring 2, cell lump collecting ring 2 tops are bulge loop, the bottom is a seat, the inwall socket connection of the outer wall of bulge loop and push pipe 1 bottom, the outer wall of cell lump collecting ring 2 lower seat is provided with anti-skidding line.
The bottom of cell lump collecting ring 2 is connected with filtration membrane 3, is connected with filtration membrane supporting ring 5 below the filtration membrane 3, and filtration membrane supporting ring 5 can be made by plastic cement or silica gel.
On the tube wall of the screw thread of bottom tube 4 or draw-in groove below annular platform is arranged, place described cell lump collecting ring 2, filtration membrane 3, filtration membrane supporting ring 5 on the annular platform.Cell lump collecting ring 2 and filtration membrane 3 are positioned at push pipe 1 and bottom tube 4.Annular platform supports the filtration membrane supporting ring in order to connect the back with push pipe.
The utility model cast-off cells prepare the cytology wax stone with the combined centrifugal pipe by push pipe 1, cell lump collecting ring 2, filtration membrane 3, bottom tube 4, filtration membrane supporting ring 5 totally five the part form.As shown in Figure 3, make push pipe, cell lump collecting ring, filtration membrane, filtration membrane supporting ring, bottom tube form a unified integral body after push pipe and bottom tube spin.
Cell lump collecting ring 2 tops are open, and the corresponding push pipe bottom of inserting with the push pipe bottom forms insert with push pipe and is connected, the bottom outer wall is provided with the anti-skidding line of vertical arrangement, be convenient to separate with push pipe in the subsequent operations, the non-stability of bottom and filtration membrane is bonding, as adopting non-setting adhesive bonding.Filtration membrane can separate under certain external force effect with the cell lump collecting ring in the subsequent operations process, is convenient to take out cell lump.
Filtration membrane 3 adopts well known materials, and by having making such as the various material plastics films in different apertures, various absorption paper, non-woven fabrics, top and the non-stability of cell lump collecting ring are bonding, as adopting non-setting adhesive bonding.Bottom periphery is the plastic cement or the silica gel filtration membrane supporting ring of supporting role, and the filtration membrane supporting ring closely is connected with filtration membrane, can not separate.Certainly, also can not use the filtration membrane supporting ring.
Annotate: cell lump collecting ring and filtration membrane, filtration membrane supporting ring material need organic solvents such as anti-dehydrated alcohol, dimethylbenzene to soak.
Bottom tube 4 closed bottoms, volume are greater than the push pipe volume, and the top has the screw thread (or draw-in groove) that is complementary with push pipe bottom thread (or draw-in groove), and there is prominent annular platform to tube chamber the screw thread of bottom tube (or draw-in groove) below.Push pipe with make the certain pressure of generation between push pipe bottom, cell lump collecting ring, filtration membrane and the bottom tube annular platform after bottom tube is connected by screw thread (or draw-in groove), and make whole tube wall have certain antiseepage stopping property, the pressure that produces between filtration membrane and the bottom tube annular platform simultaneously compresses and fixedly filtration membrane periphery plastic cement or silica gel filtration membrane supporting ring.When push pipe separated with bottom tube, push pipe, cell lump collecting ring, filtration membrane be for having certain internuncial integral body, can with push pipe jointly with the separating and take out of bottom tube.
In the operation, cell suspension injects push pipe, after centrifugal, solid-liquid separation is taken off cell lump collecting ring and filtration membrane, and cell lump promptly is positioned at it, can enter the wax stone production process jointly and handle, when embedding, take off filtration membrane, can take out complete cell lump easily.
This centrifuge tube is that medical speciality is used for separating the preparation cast-off cells and is used to make the cell lump wax stone and uses, according to what of cell quantity in the cell suspension, select to use the centrifuge tube of different bores, make the cell lump of making have suitable thickness, guarantee slice-making quality.Cell suspension (being generally the spissated cell suspension in for the first time centrifugal back) with suitable dosage during use injects push pipe, put into horizontal centrifugal whizzer, centrifugal back liquid phase composition enters bottom tube through filtration membrane, the solid phase composition is the solid matter piece of the cell lump collecting ring internal diameter cross section columnar shape of certain rule, retain on the filtration membrane, take out centrifuge tube, after separating push pipe and bottom tube, the above composition of filtration membrane takes out with push pipe, separate with bottom tube, hold anti-skidding line place, tight cell lump collecting ring bottom again, with push pipe and cell lump collecting ring, filtration membrane separates, and will contain the cell lump collecting ring of cell lump subsequently, filtration membrane is put into dewatering box jointly and is entered the water extracter dehydration, waxdip; When embedding, behind isolated cell piece collecting ring and the filtration membrane, cell lump dehydration back volume-diminished can be separated with the cell lump collecting ring easily, can embedding production standard cytology wax stone.
Embodiment recited above is described preferred implementation of the present utility model; be not that design of the present utility model and protection domain are limited; under the prerequisite that does not break away from the utility model design concept; common engineering technical personnel make the technical solution of the utility model in this area various modification and improvement all should fall into protection domain of the present utility model.

Claims (4)

1. cast-off cells prepare cell lump wax stone combined centrifugal pipe, comprise push pipe (1), it is characterized in that: the upper and lower ends opening of push pipe (1), and the bottom of push pipe (1) is threaded or draw-in groove; The bottom of push pipe (1) is connected with cell lump collecting ring (2), the upper and lower ends opening of cell lump collecting ring (2), and the bottom of cell lump collecting ring (2) is connected with filtration membrane (3); Push pipe (1) is connected with bottom tube (4) by screw thread or draw-in groove, the upper end open of bottom tube (4), lower end closed; Described cell lump collecting ring (2) and filtration membrane (3) are positioned at push pipe (1) and bottom tube (4).
2. cast-off cells according to claim 1 prepare cell lump wax stone combined centrifugal pipe, it is characterized in that: be connected with filtration membrane supporting ring (5) below the described filtration membrane (3).
3. cast-off cells according to claim 2 prepare cell lump wax stone combined centrifugal pipe, it is characterized in that: be threaded or draw-in groove on the outer wall of described push pipe (1) bottom, correspondence is threaded or draw-in groove on the inwall of described bottom tube (4), on the tube wall of the screw thread of bottom tube (4) or draw-in groove below annular platform is arranged, place described cell lump collecting ring (2), filtration membrane (3), filtration membrane supporting ring (5) on the annular platform.
4. cast-off cells according to claim 3 prepare cell lump wax stone combined centrifugal pipe, it is characterized in that: described cell lump collecting ring (2) top is bulge loop, the bottom is a seat, the inwall socket connection of the outer wall of bulge loop and push pipe (1) bottom, the outer wall of cell lump collecting ring (2) lower seat is provided with anti-skidding line.
CN2010205537829U 2010-09-30 2010-09-30 Combined centrifuge tube for preparing cell blocks by using cast-off cells Expired - Fee Related CN201825948U (en)

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CN2010205537829U CN201825948U (en) 2010-09-30 2010-09-30 Combined centrifuge tube for preparing cell blocks by using cast-off cells

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Application Number Priority Date Filing Date Title
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101992136A (en) * 2010-09-30 2011-03-30 何洪敏 Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells
CN103543057A (en) * 2013-11-13 2014-01-29 何洪敏 Combined type centrifuge tube for preparation of cytological paraffin block by using trace cast-off cells
CN103849562A (en) * 2012-12-05 2014-06-11 北京东方华辉生物医药科技有限公司 Sleeve for biological sample in-vitro purification treatment
CN104990780A (en) * 2015-07-26 2015-10-21 赵稳兴 Dedicated centrifugal tube and method for manufacturing cell blocks
WO2020168085A3 (en) * 2019-02-13 2020-09-24 The Trustees Of Columbia University In The City Of New York Medical apparatus and method for collection and preparation of biological samples
WO2021042159A1 (en) 2019-09-02 2021-03-11 Pang Tony A device for immobilising rare cells for cytology
WO2021047096A1 (en) * 2019-09-11 2021-03-18 陈俊林 Anti-contamination mycobacterium tuberculosis culture tube
US11369350B2 (en) 2012-11-20 2022-06-28 The Trustees Of Columbia University In The City Of New York Medical apparatus and method for collecting biological samples
CN116106108A (en) * 2023-02-13 2023-05-12 杭州瑞普晨创科技有限公司 Cell wax block and embedding method for frozen sections of small number of cells

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101992136B (en) * 2010-09-30 2012-09-05 何洪敏 Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells
CN101992136A (en) * 2010-09-30 2011-03-30 何洪敏 Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells
US11369350B2 (en) 2012-11-20 2022-06-28 The Trustees Of Columbia University In The City Of New York Medical apparatus and method for collecting biological samples
US11918192B2 (en) 2012-11-20 2024-03-05 The Trustees Of Columbia University In The City Of New York Medical apparatus and method for collecting biological samples
CN103849562A (en) * 2012-12-05 2014-06-11 北京东方华辉生物医药科技有限公司 Sleeve for biological sample in-vitro purification treatment
CN103849562B (en) * 2012-12-05 2015-06-10 北京东方华辉生物医药科技有限公司 Sleeve for biological sample in-vitro purification treatment
CN103543057A (en) * 2013-11-13 2014-01-29 何洪敏 Combined type centrifuge tube for preparation of cytological paraffin block by using trace cast-off cells
CN103543057B (en) * 2013-11-13 2015-12-02 何鑫 The Combined centrifuge tube of cytology wax stone is prepared for micro-cast-off cells
CN104990780A (en) * 2015-07-26 2015-10-21 赵稳兴 Dedicated centrifugal tube and method for manufacturing cell blocks
CN104990780B (en) * 2015-07-26 2017-10-17 赵稳兴 A kind of method for making cell block
WO2020168085A3 (en) * 2019-02-13 2020-09-24 The Trustees Of Columbia University In The City Of New York Medical apparatus and method for collection and preparation of biological samples
EP4025891A4 (en) * 2019-09-02 2023-10-11 Rare Cell Technologies Pty Ltd DEVICE FOR IMMOBILIZING RARE CELLS FOR CYTOLOGY
WO2021042159A1 (en) 2019-09-02 2021-03-11 Pang Tony A device for immobilising rare cells for cytology
WO2021047096A1 (en) * 2019-09-11 2021-03-18 陈俊林 Anti-contamination mycobacterium tuberculosis culture tube
CN116106108A (en) * 2023-02-13 2023-05-12 杭州瑞普晨创科技有限公司 Cell wax block and embedding method for frozen sections of small number of cells
CN116106108B (en) * 2023-02-13 2024-04-05 杭州瑞普晨创科技有限公司 Cell wax block and embedding method for frozen sections of small number of cells

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Legal Events

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C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20110511

Termination date: 20110930