CN201799135U - Split nano-fiber solid phase extraction column - Google Patents

Abstract

The utility model discloses a split-type nanofiber solid-phase extraction column, which comprises a sample storage tube (1), and is characterized in that a fiber filling tube (2) is sleeved on the lower end of the sample storage tube (1), and the fiber filling tube (2) The inside of the filling tube (2) is filled with nanofibers as column packing. The extraction column has the advantages of simplifying the operation process, improving the enrichment efficiency, shortening the operation time and the like. Compared with traditional solid-phase extraction columns, the amount of adsorbent is greatly reduced, and it is convenient and fast to make and use.

Description

Split nanofiber solid phase extraction column

technical field

The invention belongs to the technical field of purification of micro or trace substances, and in particular relates to a split nanofiber solid-phase extraction column and its application.

Background technique

Modern analysis technology is developing towards fast, micro, trace, multi-component, and high selectivity. Various new types of precision instruments emerge in an endless stream, and higher and higher requirements are put forward for the sample pretreatment process. However, 60% or more of the analysis operation time is spent on sample pretreatment, and most of the experimental errors come from the sample pretreatment process. Solid-phase extraction (Solid-Phase Extraction, referred to as SPE) is an effective means of separation and purification of trace or trace substances. It is a sample pretreatment technology developed in recent years. It is developed by combining liquid-solid extraction and column liquid chromatography. It is mainly used for the separation, purification and concentration of samples. Compared with the traditional liquid-liquid extraction method, it can improve the recovery rate of analytes, more effectively separate analytes from interfering components, reduce the sample pretreatment process, and is easy to operate. ,save time and energy. Widely used in medicine, food, environment, commodity inspection, chemical industry and other fields.

SPE is mostly used to process liquid samples to extract, concentrate and purify semi-volatile and non-volatile compounds. It can also be used for solid samples, but it must be processed into liquid first. The SPE device consists of SPE columns and accessories. SPE small column consists of three parts, column tube, sintered pad and packing. SPE accessories generally include vacuum system, vacuum pump, drying device, inert gas source, large-capacity sampler and buffer bottle. Most of the current commercial extraction columns use granular fillers (about 30-60 μm), and the column materials are mostly plastic (usually polypropylene), glass and stainless steel. 1g. When using a column solid-phase extraction device, the analyte is generally eluted and collected in a minimum volume (several milliliters) of organic solvent through steps such as activation, adsorption and extraction of the sample, and elution, and then blown off with nitrogen and concentrated to a higher concentration. Small volume for analysis. These operations are cumbersome, the enrichment effect is not obvious, and the operation time is long, which affects the test efficiency. The present invention comes to this end.

Contents of the invention

The purpose of the present invention is to provide a split-type nanofiber solid-phase extraction column, which solves the problems in the prior art that the solid-phase extraction operation is cumbersome, the enrichment effect is not obvious, and the operation time is long.

In order to solve these problems in the prior art, the technical solution provided by the invention is:

A split-type nanofiber solid-phase extraction column, including a sample storage tube, characterized in that the lower end of the sample storage tube is sleeved with a fiber filling tube, and the inside of the fiber filling tube is filled with nanofibers as a column filler.

Preferably, the sample storage tube includes upper and lower tapered tubes, and the upper and lower tapered tubes are connected into one body through the larger end of the tapered tube.

Preferably, the fiber-filled tube includes a circular tube filled with nanofibers, and the circular tube orifice is a tapered orifice that is socketed and fitted with the orifice of the tapered tube at the lower end.

Preferably, a collecting pipe is fixedly connected to the lower end of the round pipe, the collecting pipe is a tapered pipe, and a porous partition or a porous diaphragm is arranged at the connection between the collecting pipe and the round pipe.

Preferably, the upper end of the sample storage tube is airtightly connected with the pressurizing device.

Preferably, the pressurizing device is selected from a syringe or an air pump.

The present invention also provides an application of the split-type nanofiber solid-phase extraction column in the separation and purification of micro or trace substances.

The nanofiber solid-phase extraction column in the technical solution of the present invention includes a fiber filling tube and a sample storage tube. The two parts of the fiber filling tube and the sample storage tube are connected by socket (specifically, the lower end of the sample storage tube is inserted into the fiber filling tube), the inside of the fiber filling tube is filled with nanofibers, and the main part of the fiber filling tube is a round The upper opening of the circular tube is a tapered nozzle that can be nested with the sample storage tube, and the lower end of the circular tube is fixedly connected to the collecting tube. The collecting pipe can be a tapered pipe, and the pipe has a certain downward inclination angle, such as an inclination angle of 5° with the vertical direction, so that it is convenient for the liquid to flow downward into the collecting pipe, and the round pipe is connected with the collecting pipe A porous partition or a porous diaphragm can be provided at the position, and a plurality of through holes are arranged on the porous partition or porous diaphragm, on the one hand, it is convenient for the sample to pass through, on the other hand, the nanofiber is restricted in the circular tube. The sample storage tube includes an upper and lower conical tube, and the upper conical tube is a conical tube with a smaller taper, which facilitates a tighter connection with the pressurizing device.

Compared with the scheme in the prior art, the advantages of the present invention are:

The technical scheme of the present invention obtains a nanofiber solid-phase extraction column, which is used to enrich and extract samples and perform sample pretreatment by using the nanofiber solid-phase extraction column (which is a solid adsorbent). The filling method is more convenient to use, and it is suitable for mass production of nanofiber solid-phase extraction columns. The present invention also obtains a device and device for fast enrichment and pre-purification treatment of complex samples of medicine and biology, environmental samples, trace samples of poisons and pollutants.

The nanofiber solid-phase extraction column of the invention is convenient for nanofiber filling, and is easy to realize mass production, so that the production and use of the novel solid-phase extraction column based on nanofiber is more convenient. The present invention is based on the solid phase extraction technology based on nanofibers, and on the basis of SPE, the high-efficiency adsorption characteristics of nanomaterials are fully utilized, the absorption and desorption are fast, and the amount of adsorbent is greatly reduced, and the amount of desorbent can be reduced during use. A step in which the volatile solvent is concentrated. The solid-phase extraction device using nanofiber as the adsorbent has the advantages of simplifying the operation process, improving the enrichment efficiency, and shortening the operation time. Compared with the traditional solid phase extraction column, because the amount of adsorbent is greatly reduced, a new design of the column is required to meet the needs of making and using the new solid phase extraction column conveniently and quickly.

Description of drawings

The present invention will be further described below in conjunction with accompanying drawing and embodiment:

Fig. 1 is the structural representation of the nanofiber solid-phase extraction column of the embodiment of the present invention;

Fig. 2 is the internal structure diagram of the nanofiber solid phase extraction column fiber packing tube of the embodiment of the present invention;

Figure 3 is a schematic diagram of the connection of the nanofiber solid-phase extraction column in the embodiment of the present invention using manual operation;

Fig. 4 is a schematic diagram of connection of the nanofiber solid-phase extraction column in an embodiment of the present invention using a pneumatic operation method.

Among them: 1 is the sample storage tube; 2 is the fiber filling tube; 3 is the pressure device; 11 is the upper conical tube; 12 is the lower conical tube; 21 is the round tube; 22 is the collecting tube; 23 is the porous partition Plate, 4 is a rubber connecting pipe.

Detailed ways

The above solution will be further described below in conjunction with specific embodiments. It should be understood that these examples are used to illustrate the present invention and not to limit the scope of the present invention. The implementation conditions used in the examples can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not indicated are usually the conditions in routine experiments.

Embodiments shown in Figures 1 to 4, the split nanofiber solid phase extraction column includes a sample storage tube 1, the lower end of the sample storage tube 1 is sleeved with a fiber filling tube 2, and the inside of the fiber filling tube 2 is filled with Nanofibers are used as column packing. The sample storage tube 1 includes upper and lower tapered tubes 11, 12, and the upper and lower tapered tubes 11, 12 are connected into one body through the larger ends of the tapered tubes. The fiber filling tube 2 includes a circular tube 21 filled with nanofibers, and the nozzle of the circular tube 21 is a tapered nozzle that is socketed and fitted with the nozzle of the tapered tube at the lower end. The lower end of the round pipe 21 is fixedly connected with a collecting pipe 22 , the collecting pipe 22 is a tapered pipe, and the connection between the collecting pipe 22 and the round pipe 21 is provided with a porous partition 23 or a porous diaphragm.

When in use, the upper end of the sample storage tube 1 is airtightly connected with the pressurizing device 3 . Said pressurizing device 3 selects a syringe or an air pump according to different ways of use.

When assembling, first fill the nanofibers into the fiber filling tube 2, then insert the sample storage tube 1 into the fiber filling tube 2, inject the required sample into the sample storage tube 1, connect the rubber connecting tube 4 and the sample storage tube 1 connection, and finally connect the rubber connecting tube 4 with the syringe or the air pump 3, then it can be used.

As shown in Figure 3, the manual operation mode is used. When in use, the sample is injected into the sample storage tube 1, and then connected to the syringe 3 through the rubber connecting tube 4, and the syringe is pushed to perform the separation and purification of the sample; as shown in Figure 4, the pneumatic operation mode , the sample is injected into the sample storage tube 1, and then connected to the pneumatic pump 3 through the rubber connecting tube 4, and the pneumatic pump is started to increase the gas pressure in the device, so that the separation and purification of the sample can be carried out.

The above examples are only to illustrate the technical conception and characteristics of the present invention, and its purpose is to allow people familiar with this technology to understand the content of the present invention and implement it accordingly, and cannot limit the protection scope of the present invention. All equivalent changes or modifications made according to the spirit of the present invention shall fall within the protection scope of the present invention.

Claims (6)

1. split type nanofiber solid-phase extraction column comprises that sample deposits pipe (1), it is characterized in that described sample deposits pipe (1) lower end and be socketed with fiber and load pipe (2), and described fiber is loaded pipe (2) inner filling nanofiber as column packing.

2. split type nanofiber solid-phase extraction column according to claim 1 is characterized in that described sample deposits pipe (1) and comprise upper and lower end conical pipe (11,12), and described upper and lower end conical pipe (11,12) links into an integrated entity by the bigger end of conical pipe.

3. split type nanofiber solid-phase extraction column according to claim 2 is characterized in that described fiber filling pipe (2) comprises the pipe (21) of filling nanofiber, and described pipe (21) mouth of pipe is the taper mouth of pipe that cooperates with lower end conical pipe mouth of pipe socket.

4. split type nanofiber solid-phase extraction column according to claim 3, it is characterized in that described pipe (21) lower end is fixedly connected with header (22), described header (22) is a conical pipe, and described header (22) is provided with porous barrier (23) or porous septum with pipe (21) junction.

5. split type nanofiber solid-phase extraction column according to claim 2 is characterized in that described sample deposits pipe (1) upper end and airtight connection of pressue device (3).

6. split type nanofiber solid-phase extraction column according to claim 5 is characterized in that described pressue device (3) is selected from syringe or pulsometer.

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