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CN201681012U - Quick environmental water sample virus enriching device - Google Patents

Quick environmental water sample virus enriching device Download PDF

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Publication number
CN201681012U
CN201681012U CN2010201713145U CN201020171314U CN201681012U CN 201681012 U CN201681012 U CN 201681012U CN 2010201713145 U CN2010201713145 U CN 2010201713145U CN 201020171314 U CN201020171314 U CN 201020171314U CN 201681012 U CN201681012 U CN 201681012U
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China
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mouth
water sample
virus
filter assemblies
enriching
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Expired - Fee Related
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CN2010201713145U
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Chinese (zh)
Inventor
王学武
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TIANJIN OUNUO INSTRUMENT CO Ltd
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TIANJIN OUNUO INSTRUMENT CO Ltd
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Priority to CN2010201713145U priority Critical patent/CN201681012U/en
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Abstract

The utility model discloses a quick environmental water sample virus enriching device which comprises a first level filter component, a second level filter component, laminar flow enriching component, a peristaltic pump and a PLC control of the peristaltic pump, and the second level filter component has back flushing function. The virus enriching device combines the advantages of intercept-type filtering and tangential flow separation, ensures the higher virus enrichment recovery rate of large volume and high flow water samples, the back flushing procedure is introduced to effectively prevent a diaphragm component from being blocked during impurity separation process, and the virus enriching device can reliably and continuously operate to reflect the quick virus enriching characteristic. The quick environmental water sample virus enriching device has higher automation degree, has the advantages of quick virus enriching, continuous and reliable operation and convenient and economical use. The utility model also fully considers the cleaning issue of the internal pipeline, adopts disposable filter membranes and filter cores to avoid the cross contamination of samples and microorganisms from breeding and reproducing in the pipeline. The quick environmental water sample virus enriching device is particularly applicable to concentrating large water sample into small sample concentrate for lab testing.

Description

The quick viral enriching apparatus of environmental water sample
Technical field
The utility model belongs to bioanalysis test and environmental science, particularly relates to the quick viral enriching apparatus of a kind of environmental water sample.
Background technology
The safety issue of microbiological indicator all is the matter of utmost importance that threatens safe drinking water, and the survey showed that, and the microbial safety of China's drinking-water effectively ensured as yet, the microorganism phenomenon ubiquity that exceeds standard.From China's pollution of waterhead disease incidence situation in recent years, in rising trend by the epidemic situations such as aseptic encephalitis, hand-foot-and-mouth disease, acute hemorrhagic conjunctivitis and neonate's acute myocarditis that enterovirus causes.Virus much larger than bacterium, in potable water, even there is a viral unit that can detect, also has the people's of causing possibility of infection to the resistivity of conventional drinking water disinfection mode.World Health Organization's suggestion in 100~1000L potable water, detects through at present the most responsive method, and the virus that do not have exists.The concentration of disease caused by infectious water poison is often very low, relies on prior art directly to detect, and therefore the enrichment of virus is the key factor that decision detects success or failure.
It is several that the method for viral enrichment mainly contains absorption method, filtration method, the precipitation method, prize law etc. in the existing water sample.Wherein, absorption method is to utilize charged material that electronegative virus in the water body is carried out the initiatively characteristic of absorption, the virus on the sorbing material surface of flowing through is caught, and then adopt method such as centrifugal, wash-out to realize separating of virus and sorbing material.The absorption method recovery is higher, but operation steps is comparatively loaded down with trivial details, and is consuming time longer, serious dependence manual operations.
Filtration method is to make water sample pass through the filter membrane in ultra-fine aperture, utilizes the principle of detention to realize separating of virus and water body, though this method is easy to be easy-to-use, speed is very fast, the virus loss is little, but has the susceptible to plugging shortcoming of filter membrane, can't be used for the lower water sample of large volume or cleanliness factor and handle.
The precipitation method are that Xiang Shuizhong adding chemical substance makes it to form flocculation sediment, utilize the virus and the combination of precipitation to realize the purpose of enrichment, and this method is applicable to enrichment viral in the small amount of water sample, and is relatively poor to the processing power of large volume water sample.
Prize law mainly is to utilize the principle of antibody selective binding viral antigen to realize the separation of virus, though this method has high specificity, highly sensitive advantage, has the defective of length consuming time, cost height, complicated operation and has limited applying of it.
The laminar flow partition method is comparatively effective a kind of separation method in the biochemical industry, this method is that sample liquid is circulated on the filter membrane surface according to the direction that is parallel to the film surface with certain flow velocity, material permeance film less than membrane aperture flows out, material tunicle greater than membrane aperture is trapped in the surface, and with liquid stream inflow collection container, the Control Circulation number of times can obtain certain concentration ratio.But above-mentioned enrichment mode only is suitable for the separation of material in the comparatively clean liquid, and the virus that is not suitable for the environmental water sample of major diameter particles such as containing more sand grain, soil ulmin, planktonic organism is separated, show use the existing layer flow point when enriching apparatus carries out virus separation to described large volume environmental water sample its filter membrane with very fast obstruction.
In sum, at the environmental water sample of larger volume (more than the 40L), still there is not comparatively effectively quick viral enriching apparatus at present.
Summary of the invention
The purpose of this utility model is to overcome the method above shortcomings of viral enrichment in the existing water sample, and discloses the quick viral enriching apparatus of a kind of environmental water sample.
The utility model is taked following technical scheme for achieving the above object: this device is provided with the application of sample bucket, its outlet is joined with first order filter assemblies inlet, described first order filter assemblies is provided with the ground floor coarse filtration film of aperture 100 μ m and the second layer coarse filtration film of aperture 30 μ m, the outlet of first order filter assemblies and the 1st two-position three way solenoid directional control valve P mouth join, this reversal valve A mouth and second level filter assemblies inlet join, the B mouth joins with the recoil waste liquid bottle, described second level filter assemblies is provided with the filtration recoil film of aperture 10 μ m and is positioned at the pressure transducer of outlet, the outlet of second level filter assemblies and the 2nd two-position three way solenoid directional control valve P mouth join, this reversal valve A mouth and peristaltic pump one port join, B mouth and specimen bottle outlet are joined, described peristaltic pump another port and the 3rd two-position three way solenoid directional control valve P mouth join, this reversal valve A mouth and laminar flow enrichment assembly inlet join, the B mouth joins with the recoil bottle, described laminar flow enrichment assembly outlet is joined with the specimen bottle inlet, and the cleaner liquid outlet links to each other with waste liquid bottle; This device is provided with the PLC programming controller, the pressure transducer of enriching apparatus startup, stop signal and second level filter assemblies represents that the signal of filter membrane blockage inputs to the PLC programming controller, and the corresponding steering order correspondence of PLC programming controller output is connected in the driver part of the 1st, the 2nd, the 3rd two-position three way solenoid directional control valve and peristaltic pump.
The utility model can also be taked following technical measures:
Ground floor coarse filtration film and second layer coarse filtration film are the disk filter membrane in described the 1st grade of filter assemblies, and its diameter is 100mm.
Filtering the recoil film in described the 2nd grade of filter assemblies is disk recoil film, and its diameter is 30mm.
The external dimensions of described laminar flow enrichment assembly is Φ 50mm * 386mm, and molecular cut off is 60K~80K, and what filled inside is the hollow fiber filtering membrane, external diameter 0.9mm/1.3mm in its single filter membrane, total surface area 0.3m 2
The working pressure 0.12MPa of described laminar flow enrichment assembly.
The beneficial effects of the utility model are with advantage: this device combines the advantage that the formula of holding back is filtered, slipstream is separated two kinds of technology, guaranteed the higher recovery of viral enrichment in large volume, the high flow capacity water sample, introduce the back flush link in good time, can prevent effectively that the membrane module in the impurity detachment process from stopping up, can turn round continuously reliably and embody the outstanding feature of its quick viral enrichment.This enriching apparatus automaticity is higher, has quick viral enrichment, the outstanding advantage of reliable, economy easy to use continuously.The utility model has also fully taken into account the cleaning problem of internal duct, and filter membrane and filter core are disposable use, can avoid cross pollution and microorganism growing and breeding in pipeline between the sample.The utility model is particularly useful for being used for the laboratory detection after the scene is condensed into small sample concentrate sample with the large sample water sample.
Description of drawings
Fig. 1 is the utility model virus enriching apparatus example structure synoptic diagram.
Fig. 2 is the filling mode synoptic diagram of the hollow fiber filter membrane of Fig. 1 laminar flow enrichment assembly.
Fig. 3 is the viral enriching apparatus workflow block diagram of Fig. 1 embodiment.
Fig. 4 is the viral enriching apparatus cleaning process block diagram of Fig. 1 embodiment.
Number in the figure: 1 application of sample bucket, 2 first order filter assemblies, 2-1 ground floor coarse filtration film, 2-2 second layer coarse filtration film, 3 the 1st two-position three way solenoid directional control valves, 4 second level filter assemblies, 4-1 filters the recoil film, 5 recoil waste liquid bottles, 6 pressure transducers, 7 the 2nd two-position three way solenoid directional control valves, 8 peristaltic pumps, 9 specimen bottles, 10 the 3rd two-position three way solenoid directional control valves, 11 recoil bottles, 12 waste liquid bottles, 13 laminar flow enrichment assemblies, 13-1 hollow fiber filtering membrane.
Embodiment
Further specify the utility model below in conjunction with embodiment and accompanying drawing thereof.
As shown in Figure 1, viral enriching apparatus is provided with application of sample bucket 1 in the water sample that present embodiment provides, the inlet of its outlet and first order filter assemblies 2 joins, described first order filter assemblies 2 is provided with the ground floor coarse filtration film 2-1 of aperture 100 μ m and the second layer coarse filtration film 2-2 of aperture 30 μ m, ground floor coarse filtration film and second layer coarse filtration film are the disk filter membrane, and its diameter is 100mm.
The P mouth of the outlet of first order filter assemblies 2 and the 1st two-position three way solenoid directional control valve 3 joins, and the A mouth of this reversal valve and the inlet of second level filter assemblies 4 join, and the B mouth joins with recoil waste liquid bottle 5.
Be provided with the filtration recoil film 4-1 of disc in the second level filter assemblies 4, its diameter is 30mm, and the aperture is 10 μ m.The outlet of second level filter assemblies 4 also is provided with pressure transducer 6, the outlet of second level filter assemblies is simultaneously also joined with the P mouth of the 2nd two-position three way solenoid directional control valve 7, the A mouth of this reversal valve and peristaltic pump 8 diagram left side ports join, and the B mouth of this reversal valve and the outlet of specimen bottle 9 are joined.
The P mouth of described peristaltic pump 8 diagram right side ports and the 3rd two-position three way solenoid directional control valve 10 joins, and this reversal valve A mouth and laminar flow enrichment assembly 13 inlets join, and the B mouth of this reversal valve joins with recoil bottle 11.The outlet of described laminar flow enrichment assembly 13 and the inlet of specimen bottle 9 join.The cleaner liquid outlet C of laminar flow enrichment assembly 13 links to each other with waste liquid bottle 12.
As shown in Figure 2, the external dimensions of laminar flow enrichment assembly is Φ 50mm * 386mm, and working pressure 0.12MPa, molecular cut off are 60K~80K, and what filled inside is the hollow fiber filtering membrane, external diameter 0.9mm/1.3mm in its single filter membrane, total surface area 0.3m 2
Among the embodiment, the 2nd two-position three way solenoid directional control valve the 7, the 3rd two-position three way solenoid directional control valve 10 is that internal diameter is the latex flexible pipe of 8mm with the connecting line of peristaltic pump 8 corresponding ports, and it is the hard teflon pipe of 5mm~8mm that other connecting line all adopts internal diameter.
This device is provided with PLC programming controller (not shown), the signal that recoil film 4-1 blockage is filtered in the expression of pressure transducer 6 outputs of the startup of enriching apparatus, stop signal and second level filter assemblies 4 inputs to the PLC programming controller, the corresponding steering order correspondence of PLC programming controller output is connected in the driver part of the 1st, the 2nd, the 3rd two-position three way solenoid directional control valve and peristaltic pump, and its workflow as shown in Figure 3.The purpose of this flow process is to keep under the situation viral in the water sample as far as possible it is tentatively filtered, and gets rid of most of impurity.Viral enrichment flow process comprises this fault eliminating step of preliminary filtration, two basic steps of viral enrichment and counterflush in the water sample.
Preliminary filtering process shown in Fig. 3 process flow diagram is: at first inject in environmental water sample application of sample bucket and treat the enrichment water sample, add clean water in the recoil bottle.Start this device switch then, under the control of PLC intelligent program, device is closed the B mouth of the 1st, the 2nd and the 3rd two-position three way solenoid directional control valve, open A, the P mouth of the 1st, the 2nd and the 3rd two-position three way solenoid directional control valve, and the flow direction of peristaltic pump is set at the 1-2 positive flow, at this moment, environmental water sample will be under the effect of peristaltic pump, pass through first order filter assemblies, second level filter assemblies and laminar flow enrichment assembly successively, contain the water sample that filters out impurities, contains virus and flow into specimen bottle, cleaner liquid enters waste liquid bottle.
Virus enrichment flow process is: at first close the 1st, the B mouth of the P mouth of the 2nd two-position three way solenoid directional control valve and the 3rd two-position three way solenoid directional control valve, open the A of the 2nd two-position three way solenoid directional control valve simultaneously, the A of P mouth and the 3rd two-position three way solenoid directional control valve, the P mouth, the flow direction of setting peristaltic pump is the 1-2 positive flow, at this moment, water sample through coarse filtration and smart filter will be no longer through the 1st, the cascade filtration assembly, only between specimen bottle and laminar flow enrichment assembly, repeatedly circulate, whenever carry out 1 circulation, all discharge a certain amount of cleaner liquid, viral suspension in specimen bottle reaches the final objective volume, stop the enrichment circulation this moment, viral enrichment flow process is finished.
The counterflush flow process is: this flow process purpose is stopped up the filtration capacity of recovery component for getting rid of under the situation that takes place to stop up at second level filter assemblies.The and the outlet of level filter assemblies is connected with pressure transducer, when the filtration recoil film of this assembly stops up, pressure can raise unusually, this is surveyed by pressure transducer unusually, at this moment, intelligent control program will close the 1st, the B mouth of the P mouth of the 2nd two-position three way solenoid directional control valve and the 3rd two-position three way solenoid directional control valve, open the A of the 2nd two-position three way solenoid directional control valve, the A of B mouth and the 3rd two-position three way solenoid directional control valve, the P mouth, peristaltic pump flows to and is set at the 2-1 reverse direction flow, at this moment, the pure water that this device will extract in the recoil bottle washes the recoil of the filtration in the filter assemblies of second level film, flushing waste drains into the recoil waste liquid bottle, recovers normal until the force value that pressure transducer records.
As shown in Figure 4, must clean whole device after viral enrichment finishes in each water sample, avoiding causing the cross pollution between the water sample, and prevent microorganism growing in pipeline when long time stored.This cleaning process is to finish automatically under the intelligent control program control of device.Cleaning process is: at first inject 100ppm Peracetic acid 10L in environmental water sample application of sample bucket, take off the filter membrane of first and second grade filter assemblies, close the B mouth of the 1st, the 2nd, the 3rd two-position three way solenoid directional control valve, open A, the P mouth of each valve, the flow direction of setting peristaltic pump is 1-2, and cleaning fluid will fully clean pipeline under the driving of peristaltic pump this moment.
The effect of viral enriching apparatus in sample is good in the water sample of present embodiment, the virus recovery is more than 40%, sample water sample processing power can reach more than the 50L/h, and in use not having the line clogging situation substantially occurs, after enrichment finishes, cleaning performance is good, for follow-up enrichment is got ready.

Claims (5)

1. quick viral enriching apparatus of environmental water sample, it is characterized in that: this device is provided with the application of sample bucket, its outlet is joined with first order filter assemblies inlet, described first order filter assemblies is provided with the ground floor coarse filtration film of aperture 100 μ m and the second layer coarse filtration film of aperture 30 μ m, the outlet of first order filter assemblies and the 1st two-position three way solenoid directional control valve P mouth join, this reversal valve A mouth and second level filter assemblies inlet join, the B mouth joins with the recoil waste liquid bottle, described second level filter assemblies is provided with the filtration recoil film of aperture 10 μ m and is positioned at the pressure transducer of outlet, the outlet of second level filter assemblies and the 2nd two-position three way solenoid directional control valve P mouth join, this reversal valve A mouth and peristaltic pump one port join, B mouth and specimen bottle outlet are joined, described peristaltic pump another port and the 3rd two-position three way solenoid directional control valve P mouth join, this reversal valve A mouth and laminar flow enrichment assembly inlet join, the B mouth joins with the recoil bottle, and described laminar flow enrichment assembly outlet is joined with the specimen bottle inlet, and the cleaner liquid outlet links to each other with waste liquid bottle; This device is provided with the PLC programming controller, the pressure transducer of enriching apparatus startup, stop signal and second level filter assemblies represents that the signal of filter membrane blockage inputs to the PLC programming controller, and the corresponding steering order correspondence of PLC programming controller output is connected in the driver part of the 1st, the 2nd, the 3rd two-position three way solenoid directional control valve and peristaltic pump.
2. the quick viral enriching apparatus of environmental water sample according to claim 1 is characterized in that: ground floor coarse filtration film and second layer coarse filtration film are the disk filter membrane in described the 1st grade of filter assemblies, and its diameter is 100mm.
3. the quick viral enriching apparatus of environmental water sample according to claim 1 is characterized in that: filtering the recoil film in described the 2nd grade of filter assemblies is disk recoil film, and its diameter is 30mm.
4. the quick viral enriching apparatus of environmental water sample according to claim 1, it is characterized in that: the external dimensions of described laminar flow enrichment assembly is Ф 50mm * 386mm, molecular cut off is 60K~80K, what filled inside is the hollow fiber filtering membrane, external diameter 0.9mm/1.3mm in its single filter membrane, total surface area 0.3m 2
5. the quick viral enriching apparatus of environmental water sample according to claim 1 is characterized in that: the working pressure 0.12MPa of described laminar flow enrichment assembly.
CN2010201713145U 2010-04-27 2010-04-27 Quick environmental water sample virus enriching device Expired - Fee Related CN201681012U (en)

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102235948A (en) * 2010-04-27 2011-11-09 天津市欧诺仪器仪表有限公司 Quick virus enrichment method and enrichment device for environmental water sample
CN102654436A (en) * 2011-03-04 2012-09-05 华东理工大学 Fluidized bed enrichment device and application method thereof
CN104745464A (en) * 2013-12-31 2015-07-01 牛刚 Full-automatic microbiological detection enrichment system and enrichment method thereof
CN104771935A (en) * 2015-04-08 2015-07-15 北京普立泰科仪器有限公司 Solid-phase extraction device
CN106010955A (en) * 2016-07-22 2016-10-12 牛刚 Sterile cabin drug sterile examination gathering working vehicle
CN106501253A (en) * 2017-01-13 2017-03-15 北京瑞升特科技有限公司 A kind of volatile phenol detecting system in gas
CN106980003A (en) * 2017-05-08 2017-07-25 华电水务控股有限公司 A kind of surface water quality device for fast detecting and its application method
CN108913544A (en) * 2018-08-02 2018-11-30 军事科学院军事医学研究院环境医学与作业医学研究所 Pathogenic microorganism efficiently concentrating device and its enrichment method in a kind of water environment
CN112812956A (en) * 2021-02-05 2021-05-18 北京埃鲁克技术检测有限责任公司 Pretreatment equipment for rapid enrichment of viruses in water body
CN114088505A (en) * 2021-11-18 2022-02-25 光大水务科技发展(南京)有限公司 Pretreatment device system of water treatment water quality detection instrument and working method thereof
CN115582229A (en) * 2022-09-27 2023-01-10 深圳赛桥生物创新技术有限公司 Clogging detection method and device

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102235948A (en) * 2010-04-27 2011-11-09 天津市欧诺仪器仪表有限公司 Quick virus enrichment method and enrichment device for environmental water sample
CN102235948B (en) * 2010-04-27 2013-06-19 天津市欧诺仪器仪表有限公司 Quick virus enrichment method and enrichment device for environmental water sample
CN102654436A (en) * 2011-03-04 2012-09-05 华东理工大学 Fluidized bed enrichment device and application method thereof
CN102654436B (en) * 2011-03-04 2014-08-27 华东理工大学 Fluidized bed enrichment device and application method thereof
CN104745464A (en) * 2013-12-31 2015-07-01 牛刚 Full-automatic microbiological detection enrichment system and enrichment method thereof
CN104745464B (en) * 2013-12-31 2016-09-14 牛刚 Full automatic microorganism detection enrichment system and enrichment method thereof
CN104771935A (en) * 2015-04-08 2015-07-15 北京普立泰科仪器有限公司 Solid-phase extraction device
CN104771935B (en) * 2015-04-08 2016-08-17 北京普立泰科仪器有限公司 A kind of solid-phase extraction device
CN106010955A (en) * 2016-07-22 2016-10-12 牛刚 Sterile cabin drug sterile examination gathering working vehicle
CN106010955B (en) * 2016-07-22 2018-12-11 牛刚 Sterile cabin pharmaceutical sterile test is enriched with Operation Van
CN106501253A (en) * 2017-01-13 2017-03-15 北京瑞升特科技有限公司 A kind of volatile phenol detecting system in gas
CN106980003A (en) * 2017-05-08 2017-07-25 华电水务控股有限公司 A kind of surface water quality device for fast detecting and its application method
CN108913544A (en) * 2018-08-02 2018-11-30 军事科学院军事医学研究院环境医学与作业医学研究所 Pathogenic microorganism efficiently concentrating device and its enrichment method in a kind of water environment
CN112812956A (en) * 2021-02-05 2021-05-18 北京埃鲁克技术检测有限责任公司 Pretreatment equipment for rapid enrichment of viruses in water body
CN114088505A (en) * 2021-11-18 2022-02-25 光大水务科技发展(南京)有限公司 Pretreatment device system of water treatment water quality detection instrument and working method thereof
CN115582229A (en) * 2022-09-27 2023-01-10 深圳赛桥生物创新技术有限公司 Clogging detection method and device

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