CN201607421U - Plane biological detection test piece - Google Patents

Abstract

The utility model provides a plane biological detection test piece, which comprises a slide glass, wherein a conductive circuit formed in a vapor deposition mode is arranged on the slide glass, and the front end and the rear end of the conductive circuit are sequentially provided with a detection electrode end and an electrode circuit end which are communicated with each other; a ferment reaction zone connected to the front end of the detection electrode; therefore, the test piece has the economic benefits of mass production and cost reduction, and further improves the stability of product quality and market competitiveness.

Description

Planar bioassay test strip

technical field

The utility model relates to a planar biological detection test piece, especially a test piece for detecting the physiological conditions of biological fluids, such as detecting blood sugar, cholesterol or urine in human blood. Excellent stability, precision, and very economical in preparation.

Background technique

The prolongation of the life expectancy of modern people is closely related to the development of modern medical technology. Many latent symptoms can be confirmed through the preliminary inspection of medical equipment, and thus achieve the effect of early prevention and treatment. Taking the detection of blood sugar in the human body as an example, a small amount of blood is collected from the human body, and then the blood is dripped into the blood glucose enzyme area on the reaction test strip for reaction, and then analyzed by a blood sugar detector to detect the blood sugar level of the human body. Users can deal with the initial symptoms of abnormal blood sugar levels as early as possible, and avoid serious diseases that cause related deterioration.

Existing blood glucose response test strips are shown in Figures 1 and 2. The reaction test strip 90 uses a single-sheet plastic film (such as polyimide) as a substrate 100, and then uses a conductive graphite material as a net Printing carbon ink or coating copper foil on the surface of the substrate 100 by means of photolithography and etching (Etching) to form a conductive circuit 93 that is connected to each other, and the conductive circuit 93 includes a detection electrode terminal 931 at the front stage and the rear electrode line end 932, and an enzyme reaction zone 92 (with enzyme) and a siphon channel 91 are sequentially connected to the front end of the detection electrode end 931, and then cover a loam cake 96 to expose the electrode line The end 932 is finally cut to an appropriate size.

When in use, the blood collected is in contact with the siphon channel 91, and the blood is sucked into the enzyme reaction area 92 through capillary action, and the glucose in the blood reacts with the enzyme to generate an electron flow, and the electron flow passes through the detection electrode terminal 931 to the The electrode line terminal 932, the electrode line terminal 932 is connected to a blood glucose detector 95, the blood glucose detector 95 measures the current through the electrode line terminal 932, and converts it into an electrical signal, and finally converts the electrical signal converted into a corresponding blood glucose concentration value.

Although the aforementioned existing blood glucose response test strip 90 can achieve the effect of measuring blood glucose, there are still many deficiencies in its preparation method, such as:

1. It is based on a single-sheet plastic film 100, and then forms the front-stage detection electrode terminal 931 and the rear-stage electrode line terminal 932 on the substrate by screen printing carbon ink, because the detection electrode terminal 931 And the length of the electrode line terminal 932 is longer and the surface area is larger, so it is more unstable during processing, and it is easy to cause the phenomenon of uneven resistance value;

2. In order to reduce the above unstable conditions, at present, a conductive silver ink is mostly printed on the carbon ink layer base, which can improve its stability, but due to the high cost of silver ink materials, and the thickness control requirements and drying process during printing Complexity also has many deficiencies;

The above-mentioned unfavorable conditions are well known and common in the market at present.

In addition, as in the U.S. Patent No. 7,465,597 patent case, it is a conductive circuit for making blood sugar response test strips by "light lithography"; in short, it uses light lithography on the substrate, Precisely make the designed conductive pattern into a photo mask and copy it to the substrate, and apply the principle of optical imaging to project the pattern onto the substrate. Due to the light emitted by the light source, only the part that passes through the transparent area of the mask can continue to pass through and be imaged on the surface of the substrate; and the surface of the substrate needs to be cleaned in advance, and then coated with photoresist (photo resist), so that the light passing through The light from the mask will react with the photoresist, and we usually call this step exposure; the exposed substrate needs to go through a development step, and then deposit gaseous metal on the preset surface without photoresist by evaporating metal. The conductive circuit and the masking layer with photoresist, and then remove the photoresist by chemical means (such as dissolution, acid etching), and the metal covering it will also be lifted off, and the remaining metal circuit will form the ideal The necessary conductive circuit; when it comes down, follow-up procedures, such as setting the enzyme reaction area, upper cover and cutting to the appropriate size of the finished product, etc.

Of course, the above-mentioned method of using "photolithography" to make the conductive circuit of the blood glucose test strip does not have the problem of low precision of the conductive circuit and the large surface area of the carbon ink material, which makes it difficult to control the electrical impedance value. However, due to the It is applied to the highly precise and precise micro-electromechanical and circuit board wiring industries, so its production cost is relatively high; on the other hand, it uses acid etching, and then removes the photoresist part with chemicals, so there will be Acidic residues destroy the activity of the enzyme, and when the preparation is not well controlled, it will lead to problems of enzyme degradation and environmental pollution, which is not an ideal preparation method. Therefore, how to improve the deficiency of the existing method for preparing blood glucose response test strips should be an important direction to be solved and overcome in this field.

Therefore, in view of the fact that the deficiencies of the existing planar biological detection test strips are not ideal, the author of this case started to develop its solution, hoping to develop a more accurate, stable and economical test strip. Planar biometric test strips are designed to serve the public and promote the development of this industry. After a long time of conception, this creation came into being.

Utility model content

The purpose of this utility model is to provide a kind of planar biological detection test strip, which uses plastic film as the carrier (the material of the carrier is not stretchable), and is completed in the form of a printing unit object, so that it can be produced quickly. , Economic benefits of cost reduction, and can further simplify the processing procedures, thereby improving the stability of product quality and market competitiveness.

In order to achieve the stated purpose, the utility model provides a planar biological detection test piece, which at least includes: a slide, which is made of a plastic film, the slide is printed with masking image prints, and the A conduction circuit is formed by vapor-depositing metal conductors on the masked image print, and the conduction circuit includes a detection electrode terminal and an electrode line terminal that are connected with each other; an enzyme reaction area is connected to the front end of the detection electrode terminal.

In order to achieve the above purpose, the utility model provides a planar biological detection test piece, which at least includes: a slide, which is made of a plastic film; a conductive circuit, which is arranged on the slide, and the guide The circuit further includes: a detection electrode terminal, which is formed by coating or printing conductive materials; and an electrode circuit terminal, which is connected to the detection electrode terminal, and the electrode circuit terminal is used to complete the detection electrode terminal. Afterwards, printing masking image prints on the carrier sheet, and vapor-depositing metal conductors on the masking image prints; and an enzyme reaction area, which is connected in front of the detection electrode terminals.

Wherein, the end of the detection electrode far away from the enzyme reaction area is a coupling electrode that forms upper and lower layers with evaporated metal conductors.

Wherein, the conductive material forming the detection electrode end is carbon ink or conductive silver ink.

Wherein, the carrier sheet is composed of single-layer or multi-layer plastic film bonding.

Wherein, the carrier sheet is made of PET film, PC film, PTFE film, nylon film, polycarbonate resin film, polystyrene film, styrene film, acrylonitrile film or butadiene copolymer film.

Wherein, the vapor-deposited metal conductive material forming the conductive circuit is: gold, silver, copper or aluminum metal.

The beneficial effects of the utility model:

The utility model provides a planar biological detection test piece for environmental protection, which uses printing to process masked image prints (such as non-toxic water-based ink), and is fully covered with a layer of metal conductive coating through an evaporation process. Then physically (such as washing process) remove the conductive plating block covered with masked image prints, and reveal the unshielded conductive circuit; because the whole process is prepared with physical characteristics, it can meet the environmental protection appeal of protecting the earth, and its control The impedance value is easy, the conductivity is stable, and it can improve the accuracy and sensitivity of micro-current detection for electrobiochemical reactions.

The technical means adopted by the utility model include: a carrier sheet, which is composed of plastic film, and the conductive circuit formed by evaporation process is arranged on the carrier sheet, and the front and rear sections of the conductive circuit are sequentially provided with corresponding The conducting detection electrode terminal and the electrode line terminal; an enzyme reaction zone is connected to the front end of the detection electrode terminal; a cover film is provided on the slide, and the cover film is a cover Overlay on the slide, and expose the electrode line end, for the convenience of detection.

Another technical means adopted by the utility model includes: on the detection electrode end of the conductive circuit on the slide, first use a conductive material (such as carbon ink, conductive silver ink, etc.) that is not easy to chemically change with the enzyme by coating or printing. Complete, vapor-deposit metal conductors to cover the electrode line end and the detection electrode end far away from the enzyme reaction zone, so that the electrode line end and the detection electrode end are in electrical conduction, and make the electrode line end far away from the detection electrode end. The detection electrode end of the enzyme reaction zone forms the upper and lower coupling electrodes; because the detection electrode end in contact with the enzyme reaction zone is a conductive material that is difficult to chemically change with the enzyme, the stability of its detection is higher , it is not easy to change due to environmental changes or the prolongation of storage time, and the control of its variable conductance coefficient (CV) is also easy.

Yet another technical means adopted by the utility model: the carrier sheet is composed of a sheet-like plastic film, and then completed by a large number of printing unit objects.

The planar biological detection test piece adopted by the utility model further includes: the carrier piece is composed of a whole bundle of roll-shaped plastic film, and then through the continuous printing of a large number of unit objects in roll-to-roll (Roll to Roll) After completion, it will have the economic benefits of mass rapid production and cost reduction, and can further simplify the processing procedures, thereby improving the stability of product quality and market competitiveness.

Description of drawings

FIG. 1 is a schematic structural diagram of an existing blood glucose response test strip.

Fig. 2 is a schematic diagram of the preparation of the existing blood glucose response test strip.

3A-3D are schematic diagrams of the structure of the first embodiment of the present invention.

Fig. 4 is the preparation flowchart of the first embodiment of the present utility model.

Fig. 5 is a schematic diagram of the preparation process of the first embodiment of the present invention.

Fig. 6 is a schematic diagram of the preparation and arrangement of the first embodiment of the present invention.

7A-7E are schematic diagrams of the second embodiment of the present invention.

Fig. 8 is a preparation flow chart of the second embodiment of the present invention.

[Description of drawing number comparison]

Biological test strips 10A, 10C slides 11A, 11C

Masked image print 12A, 12C Conductive circuit 13A, 13C

Detection electrode terminals 131A, 131C Electrode line terminals 132A, 132C

Enzyme reaction zone 16A, 16C

Cover film 15A, 15C Conductive coating 17A, 17C

Plastic film 31 Gravure printing wheel 32

Screen printing 321 Drying equipment 33

Vacuum evaporation machine34 Washing equipment35

Enzyme printing equipment 36 cutting machine 37

Pre-printed or coated on plastic film to form detection electrodes (20C)

Construction of Supports with Masked Image Prints (21A, 21C)

Evaporation to form conductive coating (22A, 22C)

Physical removal of conductive plated areas covered with masked image prints to reveal conductive circuits (23A, 23C)

Place the enzyme in the enzyme reaction area of the carrier plate (24A, 24C)

Attach the cover film to the carrier (25A, 25C)

Cut into finished unit objects (26A, 26C)

Detailed ways

In order to have a further understanding and understanding of the technology, features and achieved effects of the utility model, I would like to provide a better embodiment diagram and a detailed description, as follows: Please refer to Figure 3C and Figure 3D, this utility model In the first embodiment of the new planar biological detection test strip, the biological detection test strip 10A includes a slide 11A, and the slide 11A is in the form of a single-layer or multi-layer sheet or a bundled roll (In this embodiment, it is in the form of a bundled roll) continuous plastic film (such as PET film, PC film, PTFE film, Nylon film (Nylon), polycarbonate resin film (polycarbonate), polystyrene film (Polystyrenes film) ), styrene film, acrylonitrile film or butadiene copolymer film ABS (Acrylonitrile butadiene styrene) and other materials that do not have stretch properties, only those who are familiar with the above-mentioned technology can still be replaced by other materials), and the above-mentioned The sheet 11A is provided with a conduction circuit 13A formed by an evaporation procedure, and the front and rear ends of the conduction circuit 13A are sequentially provided with a detection electrode terminal 131A and an electrode line terminal 132A that are connected to each other, and the front end of the detection electrode terminal 131A is It is also connected with an enzyme reaction area 16A, in which an enzyme is arranged, and an upper cover film 15A is arranged on the slide 11A, and the electrode line terminal 132A is exposed to facilitate detection.

Please also refer to Fig. 3A and Fig. 3B, the composition of the planar biological detection test piece 10A is to print a shadow mask (Shadow Mask) 12A on the slide 11A, the shadow mask 12A 12A reveals a conductive circuit 13A print (in the present embodiment, it is in the form of negative engraving, that is, it is not coated or printed on the printed block of the detection electrode end and the printed block of the electrode line end); After the evaporation process, it is used to form a conductive coating 17A, and then the conductive coating 17A block covered with the masked image print 12A is physically removed (such as washing with water), and the detection electrode terminal 131A is exposed and the electrode terminal 132A; in a preferred embodiment, the masking image print 12A is formed by printing with non-toxic water-based ink.

Please refer to Fig. 4 to Fig. 6, based on the composition of the above-mentioned planar biological detection test piece 10A, the preparation of the present utility model includes:

(1) Construct the masked image print 12A (21A) in a continuous mass printing manner in the form of a sheet or a bundle of rolls (in this embodiment, Roll to Roll is taken as an example) (the printing method is as follows: Screen printing, or in the gravure roller printing of present embodiment); Namely get the plastic film 31 (such as PET film, PC film, PTFE film, nylon film, polycarbonate resin film, Polystyrene film, styrene film, acrylonitrile film or butadiene copolymer film and other materials that do not have stretch properties) are used as the material of the carrier sheet 11A (as shown in Figure 3A), and the carrier sheet 11A is obtained by The gravure continuous printing of gravure printing wheel 32 (or screen printing, but those who are familiar with the technology can still implement other printing methods), the gravure printing wheel 32 is provided with a plurality of screens that can accommodate non-toxic water-based ink. The target print 321, and the carrier sheet 11A is printed with a layer mask image print 12A.

(2) Evaporation to form a conductive coating (22A); after the described carrier 11A printed with a layer masking image print 12A passes through a drying program of a drying device 33, it enters the evaporation process of a vacuum evaporation machine 34. Plating procedure, the vapor deposition procedure is to pass the carrier sheet 11A through the vapor deposition roller and wind it on the winding station (not shown), then use a vacuum pump to evacuate, and heat the evaporation source to make the high-purity conductive material (such as Gold, silver, copper, aluminum and other metals) are melted at high temperature and evaporated into a gaseous state. After activating the film winding system, when the film running speed reaches a certain value, the baffle is opened to make the gaseous conductive particles move on the carrier sheet 11A The surface is deposited and cooled to form a continuous conductive plating layer 17A on the carrier 11A.

In this evaporation process, the evaporation speed of conductive materials (such as gold, silver, copper, aluminum and other metals), the moving speed of the carrier sheet 11A, and the vacuum degree in the evaporation chamber can be determined to control the evaporation process. The thickness of the conductive plating layer 17A makes the preparation quality of the detection electrode terminal 131A and the electrode line terminal 132A accurately controlled.

(3) Remove the conductive coating 17A (23A) covered with the masking image print 12A; that is, the carrier 11A that has completed the vapor deposition process will be covered with the masking image print 12A through a washing device 35 (such as washing with water). (Mask) The conductive plating layer 17A block is washed and removed, and the exposed part is the detection electrode terminal 131A and the electrode line terminal 132A.

(4) Place the enzyme in the enzyme reaction area (24A) of the carrier plate; that is, the slide 11A is followed by an enzyme printing device 36 to set the enzyme, and the enzyme is placed on the slide 11A (enzyme reaction area 16A ), the setting of the ferment can be gravure, screen printing, jet printing or dripping.

Furthermore, if the utility model is printed by gravure, the thickness of the enzyme can be set according to the depth of the gravure, so that the effect of the enzyme reaction is better and the detection is more accurate.

(5) Paste the upper cover film 15A on the carrier sheet 11A (25A); that is, continuously print adhesive materials (such as water glue, acrylic glue) on the carrier sheet 11A and relatively attach the upper cover film 15A , and expose the electrode line terminal 132A to facilitate detection.

It is worth mentioning that, the enzyme reaction zone 16A (detection electrode terminal 131A) of the plastic film 31 is set in a double facing, as shown in FIG. The arrangement of a plurality of upper cover films 15A can improve the manufacturing efficiency.

(6) Cutting into unit object finished products (26A); the plastic film 31 that is about to set up relevant composition enters a cutting machine 37 to carry out cutting procedure, and obtains a plurality of complete planar biological detection test strips 10A (blood sugar detector) finished product.

Please refer to Fig. 7A to Fig. 7E again, it is the second embodiment of the planar biological detection test piece of the present invention, the biological detection test piece 10C includes a slide 11C (the composition of the slide is the same as that of the first slide One embodiment is the same), and the slide 11C is provided with a conductive circuit 13C, and the conductive circuit 13C includes a detection electrode terminal 131C and an electrode line terminal 132C; wherein, the detection electrode terminal 131C is not easy to connect with The conductive material (such as carbon ink, conductive silver ink, etc.) that the enzyme produces chemical changes is completed by coating or printing; and then evaporated metal conductive material (such as gold, silver, copper, aluminum and other metals) covers the electrode line end 132C and the detection electrode terminal 131C far away from the enzyme reaction zone 16C, so that the electrode line terminal 132C is electrically connected to the detection electrode terminal 131C, and the detection electrode terminal 131C forms an upper and lower coupling electrode ; Because the detection electrode terminal 131C contacted with the enzyme reaction zone 16C is a stable conductive material, it will not produce chemical changes with the enzyme in the enzyme reaction zone 16C, so the stability of its detection is higher, and it is not necessary to It is easy to change due to environmental changes or prolongation of storage time, and the control of its variable conductance coefficient (CV) is also easy.

Please refer to Figure 8, which is based on the preparation of the above-mentioned second embodiment, which includes:

(1) Pre-printed or coated on the plastic film to form the detection electrode (20C): that is, take the plastic film in the form of a sheet or wound into a bundle of rolls as the material of the carrier sheet, and place it on the plastic film Printing or coating conductive materials that are not easy to chemically change with enzymes to form detection electrode terminals;

(2) Construct a carrier plate (21C) with a masked image print in a printing manner; that is, a layer of masked image print 12C is printed on the carrier sheet 11C, and the masked image print is connected to the detection electrode terminal 131C connection;

(3) Evaporation to form a conductive coating (22C); after the drying process, the carrier 11C printed with the masking image print 12C enters an evaporation process to cover the electrode line terminal 12C and away from it. The detection electrode terminal 131C of the enzyme reaction zone 16C is such that the electrode line terminal 132C is electrically connected to the detection electrode terminal 131C, and the detection electrode terminal 131C forms an upper and lower coupling electrode;

(4) Remove the conductive coating (23C) covered with the masking image print 12C; that is, the carrier 11C that has completed the vapor deposition process will be covered with the masking image print in a physical way (such as washing) through a washing device. The conductive plating layer 17C of the pattern 12C (Mask) is washed and removed, and the exposed part is the detection electrode terminal 131C and the electrode line terminal 132C.

(5) Place the enzyme in the enzyme reaction zone (24C) of the carrier plate; that is, the slide 11C is followed by an enzyme printing device 36 to set the enzyme, so that the enzyme is placed on the slide 11C (enzyme reaction zone), the setting of the ferment can be operations such as gravure, screen printing, jet printing or dripping.

(6) Paste the upper cover film 15C on the carrier sheet 11C (25C); that is, continuously print adhesive materials (such as water glue, acrylic glue) on the carrier sheet 11C and relatively attach the upper cover film 15C , and expose the electrode line terminal 132C to facilitate detection.

(7) Cutting into unit object finished products (26C); the plastic film 31 that is about to set up relevant composition enters a cutting machine to carry out the cutting procedure, and obtains a plurality of complete planar biological detection test strips 10C (blood sugar detection device) finished product.

With the help of the above structure, the utility model enables the planar biological detection test piece to form conductive characteristics through physical means, which can meet the environmental protection demands of protecting the earth, and has easy impedance control, stable conductivity, and excellent physiological measurement accuracy and sensitivity. .

In summary, the utility model has indeed met the requirements of a new patent, and a patent application has been filed according to law. It’s just that the above description is only a preferred embodiment of the utility model, and it’s not used to limit the scope of the utility model’s implementation. Therefore, all equal changes are made according to the shape, structure, characteristics and spirit of the utility model’s patent application scope. and modification, all should be included in the patent application scope of the present utility model.

Claims (7)

1. a plane formula biological detecting test piece is characterized in that, includes at least:

A slide glass, it is made of plastic sheeting, is printed with the occlusion image plating on the described slide glass, and forms the conduction road with the evaporation metal conducting objects on described occlusion image plating, and described conduction road includes detecting electrode end and the electrode wires terminal that is conducted;

A ferment reaction zone is before it is arranged in described detecting electrode end.

2. a plane formula biological detecting test piece is characterized in that, includes at least:

One slide glass, it is that plastic sheeting constitutes;

A conduction road, it is located on the described slide glass, and described conduction road further includes:

The detecting electrode end, it is with coating or printing conductive material means and forming; And

The electrode wires terminal, itself and described detecting electrode end are conducted, and described electrode wires terminal is after finishing described detecting electrode end, printing occlusion image plating on described slide glass, and on described occlusion image plating, form with the evaporation metal conducting objects; And

A ferment reaction zone is to be arranged in described detecting electrode end front end.

3. plane formula biological detecting test piece as claimed in claim 2 is characterized in that, is the coupling electrode that forms levels with the metallic conduction thing of evaporation in the end away from described ferment reaction zone on the detecting electrode end.

4. plane formula biological detecting test piece as claimed in claim 2 is characterized in that, the conductive material that forms described detecting electrode end is carbon ink or conductive silver printing ink.

5. plane formula biological detecting test piece as claimed in claim 1 or 2 is characterized in that, described slide glass is made of single or multiple lift plastic sheeting applying institute.

6. plane formula biological detecting test piece as claimed in claim 1 or 2, it is characterized in that described slide glass is constituted by PET film, PC film, PTFE film, anti-grand film, poly-carbonic acid resin film, plasticon, styrene film, vinyl cyanide film or butadiene copolymer film.

7. plane formula biological detecting test piece as claimed in claim 1 or 2 is characterized in that, the evaporation metal conductive material that forms described conduction road is: gold, silver, copper or aluminum metal.

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