CN201517993U - Micro-fluidic chip detecting device based on photoacoustic technique - Google Patents

Abstract

The utility model relates to a micro-fluidic chip detecting device based on the photoacoustic technique, comprising an incident light generating unit A, a photoacoustic detecting unit B, a signal processing and data converting unit C, a computer processing unit D and a platform translational in the X direction and in the Y direction. The preprocessed sample to be measured is added into a micro-fluidic chip for the electrophoresis, the computer processing unit sends out a signal according to the instruction, the signal is subjected to the D/A conversion by a single chip to control the high voltage of the electrophoresis, under the effect of the electric field, the samples with different mobility are separated based on different transport factors, the samples absorb the light energy by a flare detecting window to generate a photoacoustic signal, the photoacoustic signal is collected and converted into an electrical signal by an ultrasonic sensor, the photoacoustic signal received by the sensor is converted into a digital signal by an A/D converter, the digital signal is processed by a computer to determine the variety and character of the sample. The utility model has the advantages of high sensibility, low cost and convenient operation.

Description

A microfluidic chip detection device based on photoacoustic technology

technical field

The utility model relates to a detection device, in particular to a microfluidic chip detection device based on photoacoustic technology.

Background technique

Micro Total Analysis System (μ-TAS), also known as Lab-on-a-chip, is a technology that integrates sample preparation, biology and chemical reactions involved in the fields of chemistry and biology. Processes such as separation and detection are miniaturized or basically miniaturized onto a chip of a few square centimeters to complete different biological or chemical reaction processes and analyze their products. It is a micro and comprehensive system.

At present, the micro-full analysis system can be divided into two categories: chip type and non-chip type, among which the chip type is the focus of the development of this technology. According to the chip structure and working mechanism of the chip, it can be divided into microarray chip (Microarray chip) and microfluidic analysis chip (Microfluidic chip). Microarray chips, also known as biochips, are mainly based on biotechnology, using technologies such as photoconductive in-situ synthesis or micro-spotting, to orderly solidify biological macromolecules such as nucleic acid fragments, polypeptide molecules and other biological samples on a support (such as glass Sheets, silicon wafers, high molecular polymers, etc.) to form a dense two-dimensional molecular arrangement, and then hybridize with the target molecules in the labeled biological sample to determine the number of target molecules in the sample. DNA microarray chip (DNA Microarray) is the most important one in this kind of chip. Another type of chip, the microfluidic analysis chip, is mainly based on biochemistry and analytical chemistry, based on micro-electromechanical processing technology (MEMS), and characterized by a micro-pipeline network, which integrates the functions of the entire analysis laboratory, including Sampling, sample pretreatment, reaction, separation, detection, etc. are integrated on the microchip. Through the miniaturization and integration of the entire sample analysis process, the goals of high-sensitivity and rapid detection, low sample consumption, high-throughput output and online automatic operation are achieved. Looking at the performance of the two chips, since the microarray chip fixes the sample on the surface of the support, the current biochip is generally disposable and has strong biological specificity. The microfluidic analysis chip system integrates the entire analysis process, that is, the process of sample collection, pretreatment, reaction, separation, and analysis, on the microchip, so that it can be reused many times. Therefore, it has a wider range of applications than biochips. Practicality and application prospects.

Microchips have the advantages of high efficiency, rapidity, less reagent consumption, sample saving, and easy integration. The advantages of microfluidic chips also lie in: batch manufacturing, low cost, high reliability, easy operability, and good repeatability. Microfluidic chips are mainly used in gene sequencing, proteomics, concentration monitoring, new drug synthesis and screening, clinical diagnosis and other fields.

The microfluidic chip is an integrated capillary electrophoresis chip, which is the fastest-growing chip technology in microfluidic technology. At present, the main detection methods include laser-induced fluorescence detection, chemiluminescence detection, mass spectrometry detection, ultraviolet absorption detection, electrochemical detection, etc. CN2575662Y discloses a microfluidic chip detection device based on a fluorescence detection method. The disadvantage of the fluorescence detection method is that the optical equipment used is complex, bulky, expensive, and the actual price of the fluorescent agent is high.

Contents of the invention

The purpose of the utility model is to provide a microfluidic chip detection device based on photoacoustic technology with high sensitivity and convenient operation, which utilizes photoacoustic spectroscopy.

In order to solve the above technical problems, the technical solution adopted by the utility model is: a microfluidic chip detection device based on photoacoustic technology, including an incident light generation unit, a signal processing and data conversion unit and a computer processing unit, and a photoacoustic signal detection unit. The unit and the platform, the microfluidic chip is placed on the platform, and the platform can be translated bidirectionally in the X and Y directions.

The incident light generating unit includes a laser located below the platform and a focusing lens located between the platform and the laser, and the focal point of the focusing lens is located on the separation channel of the microfluidic chip.

The photoacoustic signal detection unit includes an ultrasonic sensor located above the chip.

The signal processing and data conversion unit includes a signal amplifier and a single-chip microcomputer, the signal amplifier includes an electrically connected preamplifier and a main amplifier; the single-chip computer includes an electrically connected A/D converter and a D/A converter.

The computer processing unit includes a computer, and the computer is equipped with acquisition control software and data processing software modules.

The ultrasonic sensor, the signal amplifier, the single chip microcomputer and the computer are electrically connected in sequence.

The working principle of the utility model is: the system design is based on microchip capillary electrophoresis, and the pretreated sample to be tested is added to the microfluidic chip for electrophoresis; Control the high voltage of electrophoresis to adjust the progress of electrophoresis. Under the action of the electric field, samples with different mobility will be separated according to different mobilities. After passing through the spot detection window, the sample absorbs light energy to generate a photoacoustic signal, which is then collected by an ultrasonic sensor and converted into an electrical signal. The photoacoustic signal received by the sensor is converted into a digital signal, and after computer signal processing, the type and nature of the sample to be tested can be determined.

The beneficial effects of the utility model are: compared with the traditional fluorescence detection method, the utility model has the advantages of high sensitivity, low cost, simple equipment and convenient operation.

Description of drawings

Fig. 1 is a structural block diagram of the utility model.

Detailed ways

Below in conjunction with accompanying drawing and embodiment the utility model is further described.

The embodiment is shown in Figure 1: a microfluidic chip detection device based on photoacoustic technology, including an incident light generation unit A, a signal processing and data conversion unit C, a computer processing unit D, a photoacoustic signal detection unit B and a platform 5 , the microfluidic chip 6 is placed on the platform 5, and the platform 5 can translate bidirectionally in the X and Y directions.

The incident light generating unit A includes a laser 11 located below the platform 5 and a focusing lens 12 located between the platform 5 and the laser 11 , the focal point of the focusing lens 12 is located on the separation channel of the microfluidic chip 6 .

The photoacoustic signal detection unit B includes an ultrasonic sensor 21 located above the chip.

The signal processing and data conversion unit C includes a signal amplifier 31 and a single-chip microcomputer 32; the signal amplifier 31 includes an electrically connected preamplifier and a main amplifier, and the single-chip microcomputer 32 includes an electrically connected A/D converter and a D/A converter.

The computer processing unit D includes a computer 41 . The ultrasonic sensor 21, the signal amplifier 31, the single chip microcomputer 32, and the computer 41 are electrically connected in sequence. The computer 41 is equipped with acquisition control software and data processing software modules.

The photoacoustic signal is transmitted to the ultrasonic sensor 21 placed above the chip 6 , the ultrasonic sensor 21 detects the photoacoustic signal in a forward mode, and the ultrasonic sensor 21 converts the collected photoacoustic signal into an electrical signal. After the electrical signal of the ultrasonic sensor 21 is processed by the signal amplifier 31, it is converted into a digital signal by the single-chip microcomputer 32A/D and sent to the computer processing unit D for processing, storage or display; Finally, control the high voltage of electrophoresis to adjust the progress of electrophoresis. On the one hand, the computer processing unit D is connected to the photoacoustic signal detection unit B through the signal processing and data conversion unit C, and performs final processing on the output signal; on the other hand, it also controls the electrophoretic voltage through the signal processing and data conversion unit C; through the computer After 41 processing, all the photoacoustic intensity data of the sample can be obtained, and the type and nature of the sample to be tested can be determined.

Claims (5)

1. A microfluidic chip detection device based on photoacoustic technology, comprising an incident light generation unit A, a signal processing and data conversion unit C and a computer processing unit D, characterized in that it also includes a photoacoustic signal detection unit B and a platform (5), the microfluidic chip (6) is placed on the platform (5); The incident light generating unit A includes a laser (11) located below the platform (5) and a focusing lens (12) between the platform (5) and the laser (11), the focus of the focusing lens (12) is located at the micro On the separation channel of the flow control chip (6); The photoacoustic signal detection unit B includes an ultrasonic sensor (21) located above the chip; The signal processing and data conversion unit C includes a signal amplifier (31) and a single-chip microcomputer (32); Said computer processing unit D comprises a computer (41); The ultrasonic sensor (21), the signal amplifier (31), the single chip microcomputer (32), and the computer (41) are electrically connected in sequence. 2. A microfluidic chip detection device based on photoacoustic technology according to claim 1, characterized in that: the platform (5) can be bidirectionally translated in the X and Y directions. 3. A microfluidic chip detection device based on photoacoustic technology according to claim 1, characterized in that: the signal amplifier (31) includes a preamplifier and a main amplifier electrically connected. 4. A microfluidic chip detection device based on photoacoustic technology according to claim 1, characterized in that: the single-chip microcomputer (32) includes an electrically connected A/D converter and a D/A converter. 5. A microfluidic chip detection device based on photoacoustic technology according to claim 1, characterized in that: said computer (41) is equipped with acquisition control software and data processing software modules.

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