CN201465393U - Paper Chromatography Experimental Device - Google Patents

Abstract

The utility model relates to an experimental apparatus for filter paper chromatography. A hole is drilled by taking a middle point of a connection line between a center point of a glass tank cover plate of a round chromatographic tank and any point at the edge as a center of a circle, and a conical rubber plug is inserted in the hole; the center of the rubber plug is provided with a hole passing through the upper surface and the lower surface of the rubber plug, and a long-neck sphere separating funnel is inserted in the hole; during the experimental operation of paper chromatography, two culture plates are placed on the inner bottom surface of the chromatographic tank, wherein a developing solvent is injected in one of the culture plates. The utility model has the advantages of simple structure, convenient use, no solvent and steam escape phenomenon, linear solvent front of an atlas of experimental, computable Rf and extremely high experimental success rate.

Description

Paper Chromatography Experimental Device

technical field

The utility model belongs to a paper chromatography experiment device.

Background technique

At present, the experimental device of "paper chromatography experiment" in the known biochemistry teaching generally consists of a flat glass chromatography cylinder cover plate (hereinafter referred to as the cylinder cover plate) with a diameter of about 330mm, a cylindrical glass container with an inner diameter of about 300mm ( Hereinafter referred to as the chromatography cylinder) and a petri dish with an inner diameter of 80-100 mm and a height of 15-20 mm. When in use, first place the culture dish on the inner bottom of the chromatography tank, inject 8-10 mm thick "developing agent" into the culture dish, and then place the filter paper that has been spotted and rolled into a cylinder upright on the culture dish. Next to the dish, and then cover the cylinder mouth with a cylinder cover plate to seal the chromatography cylinder, let the solvent vapor formed by the evaporation of the developer saturate the space in the chromatography cylinder and the filter paper for "balance". After the balance is over, the operator opens the cylinder cover and reaches into the chromatography cylinder, picks up the filter paper and puts it vertically into the petri dish, allows the developer to flow from bottom to top along the filter paper, and then adds the cylinder cover to seal the chromatography. Cylinder "unfolds". Once the cylinder cover is opened during the above operation, almost all the solvent vapor in the chromatography cylinder will escape. As a result, the solvent front of the experimental spectrum will be in an irregular curve and _Rf cannot be calculated, which often leads to the failure of the experiment.

Contents of the invention

The purpose of this utility model is to provide a paper chromatography experiment device, which uses a long-necked spherical separating funnel to add a developing agent when the chromatography cylinder is closed, so that the filter paper is immersed in the developing agent, so as to overcome the problem of opening the cylinder cover during operation. , almost all the solvent vapor in the chromatographic cylinder runs away, and the consequence is that the solvent front of the experimental spectrum is in an irregular curve shape and R _f cannot be calculated, which often leads to the disadvantage of experimental failure.

The utility model is composed of a chromatography tank, a petri dish, a developing agent, a filter paper, a plate cover, a conical rubber stopper and a spherical separating funnel, and is characterized in that a petri dish and a petri dish are placed on the inner bottom of the chromatography tank, and when balanced , only install the developer in the petri dish, place the filter paper that has been spotted and rolled into a cylindrical shape vertically in another petri dish without developer. The rubber stopper is inserted into the round hole of the chromatography tank cover, and the center of the truncated rubber stopper is inserted into a spherical separating funnel. The funnel neck of the separating funnel extends from the center of the cylindrical filter paper, and the lower outlet of the funnel neck is close to the When the inner bottom surface of the culture dish is unfolded, the switch of the spherical separating funnel is turned on, and the developer is allowed to flow into the culture dish from the lower liquid outlet of the funnel neck for development.

The beneficial effects of the utility model are that the structure is simple, the use is convenient, and there is no phenomenon of solvent vapor escaping, the solvent front of the experimental map is linear, R _f can be calculated, and the success rate of the experiment is extremely high.

Description of drawings

Fig. 1 is a structural diagram of a longitudinal section in the balanced progress of the utility model;

Fig. 2 is a longitudinal sectional structure diagram during the deployment of the utility model.

In the figure, 1 is a spherical separating funnel, 2 is a separating funnel switch, 3 is a cylinder cover plate, 4 is a chromatography cylinder, 5 is a petri dish, 6 is a round table-shaped rubber stopper, 7 is a funnel neck, 8 is a sampling filter paper, 9 is a petri dish, and 10 is a developer.

Detailed ways

The technical scheme adopted by the utility model to solve its technical problems is: take the midpoint of the line between the center point of the above-mentioned glass jar cover plate and any point on the edge as the center point to drill a hole with a diameter of 37mm, and insert a hole with a diameter of 40mm, bottom diameter 35mm, high 35mm frustum-shaped rubber stopper. Drill a hole through the upper and lower sides with a diameter of 10mm in the center of the rubber stopper, and insert a 300ml long-necked spherical separating funnel in the hole. When performing paper chromatography experiments, first place two petri dishes with an inner diameter of 80-100 mm and a height of 15-20 mm on the inner bottom of the chromatography tank, and inject a developer about 10 mm thick into one of the petri dishes. Place the filter paper that has been spotted and rolled into a cylindrical shape vertically in another Petri dish without developer, with the spotting end facing down. Then cover the cylinder cover to make the chromatography cylinder airtight, and make the funnel neck of the separating funnel located in the center of the cylindrical filter paper and the lower liquid outlet close to the inner bottom surface of the culture dish, close the separating funnel, and let the chromatography The solvent vapor generated by the developer in the cylinder is "balanced". After the balance is over, fill up the developing agent in the separating funnel, turn on the switch, and let the developing agent flow down into the petri dish slowly. The experiment is over.

In the "equilibrium in progress" step of the implementation example shown in Figure 1, the petri dish 5 and the petri dish 9 are placed in the chromatography cylinder 4, and the petri dish 9 is filled with a developing agent with a thickness of about 10 mm, and the cylinder cover plate 3 is covered in the chromatography cylinder. On the mouth of the cylinder 4, the frustum-shaped rubber plug 6 is inserted into the round hole of the cylinder head plate 3, and the spherical separating funnel 1 runs through the round hole in the middle of the frustum-shaped rubber plug 6, which has been sampled and rolled into a cylindrical shape. Put the filter paper 8 vertically in the Petri dish 5 without developing agent, with the sampling end facing down, cover the cylinder cover plate 3 to seal the chromatography cylinder 4, and make the funnel neck 7 of the spherical separating funnel 1 just in the cylinder In the center of the filter paper 8, the lower liquid outlet of the separatory funnel 1 is close to the inner bottom surface of the culture dish 5, and the switch 2 of the separatory funnel is closed.

In the step of "expansion in progress" in the embodiment shown in Figure 2, the petri dish 5 and the petri dish 9 are placed in the chromatography cylinder 4, and the petri dish 9 is filled with a developing agent with a thickness of about 10mm, and the cylinder cover plate 3 is covered in the chromatography cylinder. On the mouth of the cylinder 4, the frustum-shaped rubber plug 6 is inserted into the round hole of the cylinder head plate 3, and the spherical separating funnel 1 runs through the round hole in the middle of the frustum-shaped rubber plug 6, which has been sampled and rolled into a cylindrical shape. Put the filter paper 8 vertically in the Petri dish 5 without developing agent, with the sampling end facing down, cover the cylinder cover plate 3 to seal the chromatography cylinder 4, and make the funnel neck 7 of the spherical separating funnel 1 just in the cylinder In the center of the filter paper 8, the lower liquid outlet of the separatory funnel 1 is close to the inner bottom surface of the culture dish 5, and the switch 2 of the separatory funnel is closed. After the "balance" step is over, fill the developing agent in the separating funnel 1, turn on the separating funnel switch 2, let the developing agent flow into the petri dish 5 slowly along the lower liquid outlet of the funnel neck 7, when the developing agent liquid level is about When the height is 10mm, turn off the separatory funnel switch 2 and carry out "expansion" until the end of the experiment.

Claims (1)

1. A kind of paper chromatography experiment device, is made up of chromatography cylinder, Petri dish, developer, filter paper, plate cover, conical rubber stopper, spherical separating funnel, is characterized in that in the inner bottom surface of chromatography cylinder (4) Put the Petri dish (5) and the Petri dish (9) on top, when balancing, only install the developer (10) on the Petri dish (9), and place it vertically in another Petri dish (5) without the developer (10) The filter paper (8) that has been sampled and rolled into a cylinder, the chromatography cylinder (4) cover plate (3) covers the cylinder mouth of the chromatography cylinder (4), and the truncated rubber plug (6) is inserted into the chromatography cylinder (4) In the round hole of the cover plate (3), a spherical separatory funnel (1) is inserted into the center of the truncated rubber stopper (6), and the funnel neck (7) of the separatory funnel (1) passes through the cylindrical filter paper (8) The center of the funnel neck (7) extends into the center, and the lower outlet of the funnel neck (7) is close to the inner bottom surface of the petri dish (5). The lower outlet of the funnel neck (7) flows into the culture dish (5) for expansion.

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