CN201092574Y - Mainstream smoke exposing device for cigarette external toxicology - Google Patents
Mainstream smoke exposing device for cigarette external toxicology Download PDFInfo
- Publication number
- CN201092574Y CN201092574Y CNU2007200921543U CN200720092154U CN201092574Y CN 201092574 Y CN201092574 Y CN 201092574Y CN U2007200921543 U CNU2007200921543 U CN U2007200921543U CN 200720092154 U CN200720092154 U CN 200720092154U CN 201092574 Y CN201092574 Y CN 201092574Y
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- CN
- China
- Prior art keywords
- cigarette smoke
- cigarette
- gas
- vitro
- tissue culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 235000019504 cigarettes Nutrition 0.000 title claims abstract description 39
- 231100000027 toxicology Toxicity 0.000 title claims abstract description 24
- 239000000779 smoke Substances 0.000 title claims abstract description 16
- 238000012360 testing method Methods 0.000 claims abstract description 18
- 238000007599 discharging Methods 0.000 claims description 6
- 230000002110 toxicologic effect Effects 0.000 abstract description 12
- 239000007789 gas Substances 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 2
- 238000000638 solvent extraction Methods 0.000 abstract description 2
- 238000000338 in vitro Methods 0.000 abstract 5
- 238000007789 sealing Methods 0.000 abstract 3
- 238000004113 cell culture Methods 0.000 abstract 2
- 230000007547 defect Effects 0.000 abstract 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 10
- 239000003546 flue gas Substances 0.000 description 10
- 239000003517 fume Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 231100000820 toxicity test Toxicity 0.000 description 5
- 239000008187 granular material Substances 0.000 description 4
- 238000009602 toxicology test Methods 0.000 description 4
- 241000208125 Nicotiana Species 0.000 description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 231100000025 genetic toxicology Toxicity 0.000 description 2
- 230000001738 genotoxic effect Effects 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 206010025323 Lymphomas Diseases 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007541 cellular toxicity Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- PJANXHGTPQOBST-VAWYXSNFSA-N trans-stilbene Chemical group C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a device for exposing mainstream cigarette smoke of cigarette bodies in vitro toxicologically, which is characterized in that the device comprises a sealing bin and a controlling unit which is used to control the conditions in the bin, a fixed frame used to place a cell culture bottle is arranged in the sealing bin, the fixed frame sways according to a certain rate via a power driving device which is arranged on the lower portion of the fixed frame, at least more than two testing gases are respectively led into the cell culture bottle through a pipeline, and various controlling buttons are arranged on a front panel of a base of the sealing bin. The device overcomes the defects that toxicological indexes of mainstream cigarette gas particular matters on a Cambridge filter are only tested and trapped in the toxicological test of the existing cigarette smoke in vitro, simultaneously, tedious solvent extraction processes after collecting the cigarette smoke can be greatly reduced, cells can be placed in mainstream cigarette smoke to expose, the toxicological indexes in vitro are tested, and the toxicological testing indexes of the mainstream cigarette smoke in vitro are directly and fully reflected. The device for exposing mainstream cigarette smoke in vitro toxicologically has simple structure, easy making, simple operation and low cost.
Description
Technical field
The utility model belongs to tobacco field testing apparatus, particularly a kind ofly is used for the device that the external toxicology main flume of cigarette exposes, mainly be with cellular exposure under cigarette smoke, measure to carry out external toxicologic index.
Background technology
The aerosol that cigarette smoke is made up of several thousand kinds of chemical substances, wherein many components all have certain toxic action for cell or human body.And external toxicologic method is to be used for measuring toxicant potential hazard or the damage of pair cell or DNA in a short time, has advantages such as quick, cheap, easy handling.Tobacco circle is being carried out a large amount of work aspect the evaluation of tobacco product hazardness both at home and abroad, because cigarette mainstream flue gas is very complicated mixture, can cause to comprise genotoxicity and different biological respinses such as cytotoxicity, thereby adopt the single evaluation method toxicity of evaluating cigarette product effectively.The external toxicologic study of cigarette smoke mainly concentrates on the genotoxicity and the cytotoxicity testing research aspect of cigarette smoke, consistent these testing method toxicity of evaluating cigarette product effectively of admitting of tobacco toxicology expert at present.Adopt external toxicologic method can also be used to estimate single additive or additive agent mixture and cigarette product design as the influence to cigarette flue gas toxity such as filter tip ventilation, cigarette composition, obbbin Gas permeability.The external toxicologic study of cigarette mainstream flue gas comprises that mainly Salmonella reversion test is Salmonella typhimurium mutating experiment, micronucleus test, mouse lymphoma cell mutant test and toluylene red cell toxicity test at present, the object of test is the main flume granule phase substance, main flume is captured with cambridge filter, the main flume granule phase substance that will trap cambridge filter then extracts, and carries out above several toxicology test again.Such testing method will spend a large amount of time, and because the just cigarette mainstream flue gas granule phase substance of test is not tested the gaseous component in the main flume, can not reflect the external toxicologic index of main flume truly.
Summary of the invention
The purpose of this utility model is just at above-mentioned existing in prior technology problem, and custom-designed a kind of simple in structure, be easy to make, simple to operate, with low cost be used for the device that the external toxicology main flume of cigarette exposes, this device is at only testing the weak point that traps the main flume granule phase substance toxicologic index on the cambridge filter in the external toxicology test of above-mentioned cigarette smoke, can also reduce significantly simultaneously and carry out loaded down with trivial details solvent extraction process after flue gas is collected, can directly also comprehensively reflect the external toxicology test index of cigarette mainstream flue gas.
The purpose of this utility model is achieved through the following technical solutions: the device that originally is used for the external toxicology main flume exposure of cigarette comprises gas-tight silo that is provided with Cang Gai and the control unit that is used to control condition in the storehouse, in gas-tight silo, be provided with the anchor that is used to place several Tissue Culture Flasks, the Power Drive Unit that anchor is installed by its underpart waves by given pace, at least two or more test gas feed in the Tissue Culture Flask by pipeline respectively, and each push button is arranged on the front panel of gas-tight silo base.
In the utility model, the Tissue Culture Flask on the anchor divides two dischargings to put, and every discharging is put three.
By 4 times/min swing, this is the hunting frequency condition that cell is suitable for growing to anchor under Power Drive Unit drives.
For ease of operation, test gas is entered in the corresponding Tissue Culture Flask by the both sides of gas-tight silo by cigarette smoke pipeline, uncontaminated air pipeline respectively; Cang Gai uncaps on the diagonal formula, and storehouse lid front panel is provided with handle.
Advantage of the present utility model is: cell directly can be inserted in the cigarette mainstream flue gas and expose, carry out external toxicologic index and measure, and can be directly and the comprehensive external toxicology test index of reflection cigarette mainstream flue gas.This device also has simple in structure, is easy to make characteristics simple to operate, with low cost.
Description of drawings
Accompanying drawing is that axle of the present utility model is surveyed synoptic diagram.
Among the figure: 1 is the bottle anchor, and 2 is gas-tight silo, and 3 is control unit, and 4 is Cang Gai, and 5 is the temperature push button, and 6 is Tissue Culture Flask, and 7 is the cigarette smoke pipeline, and 8 is the uncontaminated air pipeline, and 9 is the flutter rate push button, and 10 is Power Drive Unit.
Embodiment
The utility model is described further below in conjunction with accompanying drawing: as shown in the figure: the device that originally is used for the external toxicology main flume exposure of cigarette comprises gas-tight silo 2 that is provided with storehouse lid 4 and the control unit 3 that is used to control condition in the storehouse, in gas-tight silo 2, be provided with the anchor 1 that is used to place Tissue Culture Flask 6, the discharging in 6 minutes two of Tissue Culture Flask on the anchor 1 is put, every discharging is put three, the Power Drive Unit 10 that anchor 1 is installed by its underpart is swung by 4 times/min, and this is the hunting frequency condition that cell is suitable for growing; Flue gas pipeline 7 and uncontaminated air pipeline 8 are fed in the Tissue Culture Flask 6 by the both sides of gas-tight silo 2 respectively, for ease of operation, storehouse lid 4 is for uncapping on the diagonal formula, storehouse lid front panel is provided with handle, and push buttons such as temperature push button 5, flutter rate push button 9 are arranged on the front panel of gas-tight silo base.
Carrying out cigarette smoke when exposing test, at first the storehouse lid 4 of fume exposure gas-tight silo 2 is closed, and regulate temperature push button 5 in the exposure condition control unit 3, make temperature in the fume exposure gas-tight silo 2 reach cell and grow temperature required 37 ℃.Open the storehouse lid 4 of fume exposure gas-tight silo 2, the Tissue Culture Flask 6 of long good cell is installed on the Tissue Culture Flask anchor 1, the main flume that smoking machine suction is produced is by the road in 7 Tissue Culture Flasks 6 that import in the fume exposure gas-tight silo 2, simultaneously uncontaminated air is by the road in 8 Tissue Culture Flasks 6 that import in the fume exposure gas-tight silo 2, as exposing control sample.The storehouse lid of holding one's breath and expose gas-tight silo 2 in the pass 4 is regulated the flutter rate push button 9 in the exposure condition control unit 3, makes Tissue Culture Flask anchor 1 flutter rate be stabilized in 4 times/min, thereby cell is fully exposed in flue gas.
Claims (5)
1. one kind is used for the device that the external toxicology main flume of cigarette exposes, it is characterized in that: it comprises gas-tight silo (2) that is provided with Cang Gai (4) and the control unit (3) that is used to control condition in the storehouse, in gas-tight silo (2), be provided with the anchor (1) that is used to place several Tissue Culture Flasks (6), anchor (1) can wave by the Power Drive Unit (10) that install its underpart, at least two or more test gas feed in the Tissue Culture Flask (6) by pipeline (7), (8) respectively, and push button (5), (9) are arranged on the front panel of gas-tight silo base.
2. the device that is used for the external toxicology main flume exposure of cigarette according to claim 1 is characterized in that: the Tissue Culture Flask (6) on the anchor (1) divides two dischargings to put, and every discharging is put three.
3. the device that is used for the external toxicology main flume exposure of cigarette according to claim 1 is characterized in that: anchor (1) is swung by 4 times/min under Power Drive Unit (10) drives.
4. the device that is used for the external toxicology main flume exposure of cigarette according to claim 1, it is characterized in that: test gas enters corresponding Tissue Culture Flask (6) by cigarette smoke pipeline (7), uncontaminated air pipeline (8) by the both sides of gas-tight silo respectively.
5. the device that is used for the external toxicology main flume exposure of cigarette according to claim 1, it is characterized in that: Cang Gai (4) uncaps on the diagonal formula, and storehouse lid front panel is provided with handle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200921543U CN201092574Y (en) | 2007-09-29 | 2007-09-29 | Mainstream smoke exposing device for cigarette external toxicology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200921543U CN201092574Y (en) | 2007-09-29 | 2007-09-29 | Mainstream smoke exposing device for cigarette external toxicology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201092574Y true CN201092574Y (en) | 2008-07-30 |
Family
ID=39900538
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007200921543U Expired - Fee Related CN201092574Y (en) | 2007-09-29 | 2007-09-29 | Mainstream smoke exposing device for cigarette external toxicology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201092574Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102086438B (en) * | 2009-12-07 | 2012-12-19 | 韩春茂 | Device and method for biological culture of cell or tissue engineering |
| CN105918261A (en) * | 2016-07-15 | 2016-09-07 | 云南中烟工业有限责任公司 | Determination device and determination method for influence of gas phase substance on motion capability of nematodes |
| CN106399076A (en) * | 2016-09-14 | 2017-02-15 | 南京大学 | Micro-fluidic gel gas-liquid interface flue gas exposing device |
-
2007
- 2007-09-29 CN CNU2007200921543U patent/CN201092574Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102086438B (en) * | 2009-12-07 | 2012-12-19 | 韩春茂 | Device and method for biological culture of cell or tissue engineering |
| CN105918261A (en) * | 2016-07-15 | 2016-09-07 | 云南中烟工业有限责任公司 | Determination device and determination method for influence of gas phase substance on motion capability of nematodes |
| CN106399076A (en) * | 2016-09-14 | 2017-02-15 | 南京大学 | Micro-fluidic gel gas-liquid interface flue gas exposing device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080730 Termination date: 20160929 |
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| CF01 | Termination of patent right due to non-payment of annual fee |