CN1960699B

CN1960699B - Keratin-binding polypeptides

Info

Publication number: CN1960699B
Application number: CN2005800167278A
Authority: CN
Inventors: H·巴尔格; T·萨布科夫斯基; H-G·勒迈尔; C·博尔施韦勒; A·普托克
Original assignee: BASF SE
Current assignee: BASF SE
Priority date: 2004-05-24
Filing date: 2005-05-24
Publication date: 2011-12-28
Anticipated expiration: 2025-05-24
Also published as: ZA200610812B; CN1960699A; DE102004025805A1

Abstract

The invention relates to novel keratin-binding protein active substances, and also to the production and use thereof.

Description

Keratin-binding polypeptides

Prior art

Vertebrate cells comprises fibril, i.e. the material be made up of keratin of a class.These keratin also are present in hair, among skin and the fingernail, and specific protein such as the desmoplakin special sequence motif by being referred to as the keratin binding structural domain is with it in conjunction with (Fontao L, Favre B, RiouS, Geerts D, Jaunin F, Saurat JH, Green KJ, Sonnenberg A, Borradori L., Interaction of the bullous pemphigoid antigen 1 (BP230) and desmoplakinwith intermediate filaments is mediated by distinct sequences within theirCOOH terminus, Mol Biol Cell.2003 May; 14 (5): 1978-92, on January 26th, 2003, electronics was open; Hopkinson SB, Jones JC., The N terminus of thetransmembrane protein BP180 interacts with the N-terminal domain ofBP230, thereby mediating keratin eytoskeleton anchorage to the cellsurface at the site of the hemidesmosome, Mol Biol Cell.2000 January; 11 (1): 277-86).

Goal of the invention

One object of the present invention is to provide to keratin or contains the new polypeptide that keratin thing such as skin or hair have high-affinity.Such polypeptide is suitable for containing the cosmetic and the drug treating of keratin structure (particularly hair and skin).

Summary of the invention

The present invention relates to be used to handle the make-up composition that contains the keratin thing, comprise at least a keratin-binding polypeptides sequence (i) in the cosmetic compatible media.

Peptide sequence (i)

Peptide sequence (i) has binding affinity to keratin.Peptide sequence (i) can be analyzed under the condition described in embodiment 8,9 and 10 with keratic the combination.

Particularly Shi Yi keratin-binding polypeptides is the sequence that is present in people's desmoplakin, or by modifying people's desmoplakin peptide sequence for example aminoacid insertion, replacement or disappearance and by it deutero-sequence.

The peptide sequence of people's desmoplakin is shown in SEQ ID NO:1.Suitable keratin binding structural domain (domain B) is the 2193rd to 2481 and the function equivalent of peptide sequence SEQ ID NO:1.Other keratin binding structural domain (domain C) is the 2606th to 2871 of peptide sequence SEQ ID NO:1 and function equivalent thereof.

The keratin binding structural domain is shown in Fig. 1.

Preferred peptide sequence (i) comprises the aminoacid sequence shown in SEQ ID NO:1.

According to the present invention, equally also comprise described concrete openly " function equivalent " and the purposes in the methods of the invention thereof of peptide sequence (i).

Be purpose meter of the present invention, " function equivalent " of described concrete openly polypeptide (i) or analog be different with it and the biological activity that additionally has an expectation for example keratin in conjunction with active polypeptide.Thereby, for instance, " function equivalent " is meant such peptide sequence, it demonstrates following combination (activity) in one of embodiment 9 or 10 described binding assays, be embodiment 9 or 10 described in conjunction with shown in the polypeptide of domain B that has SEQ ID NO:1 in measuring or domain C in conjunction with (activity) at least 10%, preferably at least 50%, especially preferred 75%, extremely preferred 90%.

The example of suitable aminoacid replacement can see the following form:

Original residue replaces example

Ala Ser

Arg Lys

Asn Gln；His

Asp Glu

Cys Ser

Gln Asn

Glu Asp

Gly Pro

His Asn；Gln

Ile Leu；Val

Leu Ile；Val

Lys Arg；Gln；Glu

Met Leu；Ile

Phe Met；Leu；Tyr

Ser Thr

Thr Ser

Trp Tyr

Tyr Trp；Phe

Val Ile；Leu

For example, as everyone knows, the serine that the natural SEQ of being present in ID NO:1 is the 2849th can be replaced by glycine, to avoid this locational phosphorylation (Fontao L, Favre B, Riou S, Geerts D, Jaunin F, Saurat JH, Green KJ, Sonnenberg A, Borradori L., Interaction of the bullous pemphigoid antigen 1 (BP230) and desmoplakinwith intermediate filaments is mediated by distinct sequences within theirCOOH terminus, Mol Biol Cell.2003 May; 14 (5): 1978-92, on January 26th, 2003, electronics was open).

According to the present invention, " function equivalent " also means to have the mutein that is different from the aminoacid of specifically mentioning but still has one of above-mentioned biological activity especially at least one sequence location of above-mentioned aminoacid sequence.Therefore, " function equivalent " comprises the mutein that can obtain by one or more aminoacid addition, replacement, disappearance and/or inversion, wherein said being modified with may be present on any sequence location, as long as can obtain having the mutein of character type of the present invention.

" function equivalent " of above-mentioned implication comprises that also " precursor " of described polypeptide, " functional deriv " of polypeptide reach " salt ".

Thus, " precursor " is for being with or without the bioactive natural or synthetic polypeptide precursor of expectation.

Term " salt " refers to the carboxylic salts and the amino acid addition salt of protein molecule of the present invention.Carboxylic salts can prepare according to himself known mode, and comprises inorganic salt for example sodium salt, calcium salt, ammonium salt, iron salt and zinc salt, and organic alkali salt, amine for example, and as triethanolamine, arginine, lysine, piperidines, or the like.The present invention relates to acid addition salt equally, inorganic acid salt for example, and example hydrochloric acid salt or sulfate, and acylate are as acetate and oxalates.

" functional deriv " of polypeptide of the present invention can prepare at the functional amino side-chain radical or on its N-terminal or C-terminal by known technology equally.For example, such derivant comprises carboxyl ester or thioesters, Carboxylamide (can by obtaining with ammonia or primary amine or secondary amine reaction); The N-acyl derivative of free amine group (by preparing) with acylation reaction; The N-alkyl derivative of free amine group (by with the alkylating agent prepared in reaction); The S-acyl derivative of free mercaptan (by preparing) with acylation reaction; The thioether of free mercaptan (by the alkylating agent prepared in reaction); Disulphide (by the reaction of free mercaptan) with for example mercaptan; The O-acyl derivative of free hydroxyl group (by preparing) with acylation reaction; Or ether (by the reaction of free hydroxyl group and alkylating agent).

" function equivalent " also comprises polypeptide and the naturally occurring variant that obtains from other organism naturally.For example, might relatively set up the homologous sequence area scope, and determine enzyme of equal value based on specific requirement of the present invention by sequence.

For example, " function equivalent " comprises the fragment of the polypeptide of the present invention with expectation biological function equally, preferred single structure territory or sequence motifs.

In addition, " function equivalent " can be fusion rotein, comprise one of aforementioned polypeptides sequence or by its deutero-function equivalent, and another different heterologous sequence on function with it at least, described heterologous sequence is functional N-terminal with it or C-terminal connects (that is, fusion rotein partly being had the mutual function damage of ignoring).For example, the limiting examples of such heterologous sequence has signal peptide or enzyme.

" function equivalent " included equally in the present invention is the described proteinic homologue that specifically discloses.According to Pearson and Lipman algorithm (Proc.Natl.Acad, Sci. (USA) 85 (8), 1988, calculating 2444-2448), these homologues and described concrete disclosed aminoacid sequence have at least 50%, preferably at least 75%, particularly at least 85%, 90%, 95% or 99% homology for example.The percent homology of homology polypeptide of the present invention is meant the homogeneity percentage ratio based on the amino acid residue of the total length of one of specifically described aminoacid sequence of this paper especially.

With regard to possible protein glycosylation, " function equivalent " of the present invention comprises the protein type of deglycosylation defined above or glycosylation form, and by changing the modified forms that the glycosylation pattern obtains.

With regard to possible protein phosphorylation effect, " function equivalent " of the present invention comprises the protein type of dephosphorylation defined above or phosphorylation form, and by changing the modified forms that the phosphorylation pattern obtains.

The homologue of polypeptide of the present invention (i) can produce by mutation, as point mutation or protein truncate.

The homologue of polypeptide of the present invention can be by for example combinatorial library evaluation of truncated mutant of screening mutant.For example, the protein variants library can be by the preparation of combinatorial mutagenesis on nucleic acid level, and for example enzymatic connects synthetic oligonucleotide mixture.There are a lot of methods to can be used for preparing potential homologue library from degenerate oligonucleotide sequence.In automatic dna synthesizer, can carry out the chemosynthesis of degeneracy gene order, then synthetic gene is connected in the suitable expression vector.Use one group of degeneracy gene, all sequences of the potential protein sequence of one group of expectation of codified might be provided in a kind of mixture.The method that is used for synthetic degenerate oligonucleotide be the technical staff known (as Narang, S.A. (1983) Tetrahedron 39:3; Itakura etc., (1984) Annu.Rev.Biochem.53:323; Itakura etc., (1984) Science 198:1056; Ike etc., (1983) Nucleic Acids Res.11:477).

Several Methods known in the art is used in by screening-gene product in the prepared combinatorial library of point mutation or truncate, and the gene outcome that has selected character at the cDNA library screening.These technology can be suitable for the gene library that the combinatorial mutagenesis of rapid screening by homologue of the present invention produces.Be used to screen the technology of normal use of a large amount of gene libraries of pending high throughput analysis, comprise gene library is cloned in the replication form expression vector, vector library with gained transforms suitable cell, and under such condition, express combination gene, expect under the described conditions that wherein active detection can promote the separation of carrier, and the gene of the described detection product of described vector encoded.Recurrence assemblage mutation (REM), a kind of technology that improves functional mutants frequency in the library can be united with screening test and is used to identify homologue (Arkin and Yourvan (1992) PNAS 89:7811-7815; Delgrave etc., (1993) Protein Engineering 6 (3): 327-331).

The particularly advantageous embodiment of the present invention is the peptide sequence (i) that comprises at least a following peptide sequence:

A) the 2193rd to 248 peptide sequence (domain B) among the SEQ ID NO:1;

B) the 2606th to 2871 peptide sequence (domain C) among the SEQ ID NO:1;

C) compare with (a), go up to the adorned peptide sequence of 60% aminoacid;

D) compare with (b), go up to the adorned peptide sequence of 50% aminoacid;

Condition be peptide sequence (c) or keratin (d) in conjunction with reach the peptide sequence (a) in the test of embodiment 9 or 10, surveyed or (b) institute's indicating value at least 10%.In this connection, domain B or C refer to the keratin binding structural domain of above-mentioned people's desmoplakin (SEQ ID NO:1).Thus, amino acid modified finger aminoacid replacement, insertion and disappearance or this three kinds of possible combination in any.

The preferred peptide sequence (i) that uses is that the organism of expecting is had the sequence of high specific affinity.Correspondingly, use for cosmetics for skin, the preferred peptide sequence (i) that adopts is the sequence that the application on human skin keratin is had special high-affinity.Use for hair cosmetic composition, preferred peptide sequence is the sequence that human hair keratin is had special high-affinity.

Correspondingly, for the application of house pet aspect, except that described peptide sequence (SEQ ID NO:1), preferred peptide sequence (i) is that corresponding keratin (for example Canis animals keratin or felid keratin) is had the sequence of special high-affinity.

Yet, in effector molecule of the present invention, also might use more than one peptide sequence (i), for example the application on human skin keratin is had the sequence (i) of high binding affinity, together with the sequence (i) that human hair keratin is had high-affinity.For example, same peptide sequence (i) that also might sequential connection multicopy is to realize higher combination.

Suitable keratin-binding polypeptides sequence (i) is known.For example, the desmoplakin and plectin (the Fontao L that comprise the keratin binding structural domain, Favre B, Riou S, Geerts D, Jaunin F, Saurat JH, Green KJ, Sonnenberg A, Borradori L., Interaction of thebullous pemphigoid antigen 1 (BP230) and desmoplakin with intermediatefilaments is mediated by distinct sequences within their COOH terminus, Mol Biol Cell.2003 May; 14 (5): 1978-92.2003 electronics on January 26 is open; Hopkinson SB, Jones JC., The N terminus of the transmembrane proteinBP180 interacts with the N-terminal domain of BP230, thereby mediatingkeratin cytoskeleton anchorage to the cell surface at the site of thehemidesmosome, Mol Biol Cell.2000 January; 11 (1): 277-86).

For example, the Vector NTI 8 (2002 year JIUYUE 25 day version) of the program that uses a computer as being provided by InforMax Inc by comparing this class known protein matter sequence, might draw and identify such zone.

Other the suitable peptide sequence (i) that human keratin is had good combination is to show the sequence area of high homology or sequence homogeneity in comparison, and can be considered the consensus sequence of keratin binding structural domain.

Preferred especially following sequence area:

Domain B (KBD-B): among the peptide sequence SEQ ID NO:1 the 2193rd to 2448;

Domain B (KBD-B): among the peptide sequence SEQ ID NO:1 the 2209th to 2448;

Domain C (KBD-C): among the peptide sequence SEQ ID NO:1 the 2606th to 2871;

Domain C (KBD-C): among the peptide sequence SEQ ID NO:1 the 2616th to 2871;

Domain C (KBD-C): among the peptide sequence SEQ ID NO:1 the 2616th to 2811;

Domain C (KBD-C): among the peptide sequence SEQ ID NO:1 the 2606th to 2871.

For example, the 2849th serine can be replaced by glycine among the known natural SEQ of the being present in ID NO:1, to avoid this locational phosphorylation, thereby guarantee that domain C is bonded to corresponding keratin (Fontao L, Favre B, Riou S, Geerts D, Jaunin F, Saurat JH, Green KJ, Sonnenberg A, Borradori L., Interaction of the bullouspemphigoid antigen 1 (BP230) and desmoplakin with intermediatefilaments is mediated by distinct sequences within their COOH terminus, Mol Biol Cell.2003 May; 14 (5): 1978-92, on January 26th, 2003, electronics was open).

If expectation peptide sequence (i) has special good binding to the keratin that comes from non-human being's body, selected suitable sequence motifs is preferably from the sequence of keratin conjugated protein (as the desmoplakin or the plectin of suitable organism).

Fig. 2 has shown the comparison of keratin binding molecule.

If expectation also can easily separate from keratin according to keratin-binding polypeptides of the present invention (i) once more.For this purpose, what might adopt is: for example, washing keratin, by this with keratin-binding polypeptides (i) from its with keratic existing the combination cement out, and carry out saturated with the keratin in the cleaning mixture.Perhaps, also might be used to wash off keratin-binding polypeptides (i) with high-load detergent (as SDS) washing.

In people's cosmetics (particularly skin, fingernail and hair-care), animal care, leather nursing and leather processing, has application fields according to keratin-binding polypeptides of the present invention (i).

(i) is preferred for cosmetics for skin according to keratin-binding polypeptides of the present invention.They allow the high concentration and the long-acting time of skin nursing or skin care effector.

The suitable adjuvant and the additive that are used to produce hair cosmetic product, fingernail cosmetic product or skin cosmetic product are well known to those skilled in the art, and can be referring to the cosmetics handbook, Schrader for example, the ultimate principle and the preparation of Grundlagen und Rezepturen der Kosmetika[cosmetics], H ü thig Verlag, Heidelberg, 1989, ISBN 3-7785-1491-1.

According to make-up composition of the present invention can be cutization composition for cosmetics, fingernail make-up composition, hair make-up composition, dermatosis compositions, health compositions or pharmaceutical composition.

Preferably, the compositions according to the present invention form that is gel, foam, spray, unguentum, cream, Emulsion, suspensoid, lotion, emulsion or paste.If expectation also can be used liposome or microsphere.

Can additionally comprise cosmetic and/or dermatosis active component and adjuvant according to cosmetic of the present invention or pharmaceutically active compositions.

Preferably, cosmetic composition according to the present invention comprises at least a keratin-binding polypeptides sequence (i) and at least a different with it component as hereinbefore defined, and wherein said component is selected from the cosmetic active component, emulsifying agent, surfactant, antiseptic, aromatic oil, thickening agent, polymeric hair, hair and skin conditioner, graft polymers, the polymer that contains water solublity or dispersibility silicones, light protective agent, brightening agent, gelatinizing agent, nursing agent, coloring agent, stain, tanning agent, dyestuff, pigment, concentration regulator, humidizer, fatting agent (re-fatting agents), collagen, protolysate, lipid, antioxidant, antifoaming agent, antistatic additive, emollient and softening agent.The keratin-binding polypeptides active component also can be present in the cosmetic product with capsule formulation.

Antioxidant preferably is selected from aminoacid (as glycine, histidine, tyrosine, tryptophan) and derivant, imidazoles (as urocanic acid) and derivant thereof, peptide such as D, the L-carnosine, the D-carnosine, L-carnosine and their derivant (as anserine), carotenoid, carotene is (as beta-carotene, lycopene) and their derivant, chlorogenic acid and derivant thereof, thioctic acid and derivant thereof (as dihydrolipoic acid), aurothioglucose, propylthiouracil and other mercaptan are (as thioredoxin, glutathion, cysteine, cystine, cystamine and glycosyl ester thereof, the N-acetonyl ester, methyl ester, ethyl ester, propyl ester, pentyl ester, butyl ester and lauryl, the palmityl ester, grease, γ-Ya oleoyl ester, cholesteryl ester and glyceride) and their salt, dilauryl thiodipropionate, distearyl thiodipropionate, thio-2 acid and their derivant (ester, ether, peptide, lipid, nucleotide, nucleoside and salt), and sulfoxide amine (sulfoximine) chemical compound of extremely low tolerance dose (as pmol to μ mol/kg) is (as buthionine sulfoximine, homocysteine sulfoxide amine, fourth methyllanthionine sulfone, five thionine sulfoxide amine, six thionine sulfoxide amine, seven thionine sulfoxide amine), also has (metal) chelating agen (as alpha-hydroxy fatty acid, Palmic acid, phytic acid, lactotransferrin), alpha-hydroxy acid is (as citric acid, lactic acid, malic acid), humic acid, bile acid, bile extract, bilirubin, biliverdin, EDTA and their derivant, unsaturated fatty acid and derivant thereof are (as gamma-Linolenic acid, linoleic acid, oleic acid), folic acid and derivant thereof, ubiquinone and pantothenylol and their derivant, vitamin C and derivant thereof are (as sodium ascorbate, ascorbyl palmitate, magnesium ascorbyl phosphate, the ascorbic acid acetate), tocopherol and derivant thereof are (as Vitamin E acetate, tocotrienol), vitamin A and derivant thereof (vitamin A palmitate), and the coniferyl benzoate of benzoin resin, rutinic acid and their derivant, the a-glycosyl rutin, ferulic acid, the furfurylidene glucitol, carnosine, butylated hydroxytoluene, Butylated hydroxyanisole, nor-dihydroguaiaretic acid phenolic acid, nordihydroguaiaretic acid, trihydroxybutyrophenone, uric acid and derivant thereof, mannose and derivant thereof, zinc and derivant thereof are (as ZnO, ZnSO ₄), selenium and derivant (as selenomethionine), stilbene and derivant thereof (as stilbene oxide, trans-stilbene oxide).

In such preparation, common thickening agent is crosslinked polyacrylic acid and derivant thereof, polysaccharide and derivant thereof, as xanthan gum, agar, alginate or methylcellulose, cellulose derivative is as carboxymethyl cellulose or hydroxyl carboxymethyl cellulose, aliphatic alcohol, monoglyceride and fatty acid, polyvinyl alcohol and polyvinylpyrrolidone.The preferred nonionic thickener that uses.

For example, suitable cosmetic and/or dermatosis active component are painted active component, the skin and hair pigment agent, stain, tanning agent, brightening agent, the keratin hardening material, the antimicrobial acivity composition, lightscreening agent (photofilter) active component, the repellant active component, material with congested effect, material with keratolysis and ceratoplasty effect, dandruff removing agent active component, antiinflammatory, material with keratinization effect, active component with effect of antioxidation or removing free radical, increase the material of moisture of skin or maintenance skin wet, the stuffing active component, anti-erythema or anti-allergy active component, branched chain fatty acid such as 18-methyl arachic acid, and their mixture.

For example, artificial tanning and be suitable for needn't be with ultraviolet rays nature or manually irradiation and the active component of tanning has dihydroxyacetone, alloxan and Endocarpium Juglandis extract.Suitable keratin hardening material normally also can be used as the active component of antiperspirant, for example aluminium potassium sulfate, polymeric aluminum chloride, aluctyl. or the like.

The antimicrobial acivity composition is used for the elimination of micro-organisms or suppresses its growth, also can be used as the deodorization material that reduces body odor formation or intensity thereby both can be used as antiseptic.For example, they comprise and well known to a person skilled in the art conventional preservatives, for example para hydroxybenzene methyl ester, imidazolinyl carbamide, formaldehyde, sorbic acid, benzoic acid, salicylic acid or the like.For example, such deodorization material has zinc ricinoleate, triclosan (triclosan), endecatylene alkylolamides (undecylenic alkylolamides), triethyl citrate, chlorhexidine or the like

Following table is listed suitable antiseptic and the E coding thereof that preferably uses according to the present invention.

E?200	Sorbic acid	E?227	Calcium bisulfite
				E?201	Sodium sorbate	E?228	Potassium acid sulfite
E?202	Potassium sorbate	E?230	Biphenyl (diphenyl)
				E?203	Calcium sorbate	E?231	O-phenyl phenol
E?210	Benzoic acid	E?232	O-Phenylphenol Sodium salt tetrahydrate
				E?211	Sodium benzoate	E?233	Thiabendazole
E?212	Potassium Benzoate	E?235	Natamycin
				E?213	Calcium benzoate	E?236	Formic acid
E?214	Ethylparaben	E?237	Sodium formate

E?215	Nipagin A sodium	E?238	Calcium formate
				E?216	The p-hydroxybenzoic acid n-propyl	E?239	Hexamethylenetetramine
E?217	P-hydroxybenzoic acid n-propyl sodium	E?249	Potassium nitrite
				E?218	Methyl parahydroxybenzoate	E?250	Sodium nitrite
E?219	Sodium Methyl Hydroxybenzoate	E?251	Chile saltpeter
				E?220	Sulfur dioxide	E?252	Potassium nitrate
E?221	Sodium sulfite	E?280	Propanoic acid
				E?222	Sodium sulfite	E?281	Sodium propionate
E?223	Sodium disulfide	E?282	Calcium propionate
				E?224	Curing potassium	E?283	Potassium propionate
E?226	Calcium sulfite	E?290	Carbon dioxide

According to the present invention same be suitable for antiseptic or the antiseptic adjuvant that is generally used for cosmetics arranged, for example dibromo dicyanobutane (2-bromo-2-bromomethyl glutaronitrile), 3-iodo-2-propynyl butyl carbamate, 2-bromo-2-nitropropane-1,3 glycol, imidazolinyl carbamide, 5-chloro-2-methyl-4-isothiazoline-3-ketone, 2-chloroacetamide, Benzalkonii Chloridum and benzyl alcohol+formaldehyde donor.

Be suitable for equally as antiseptic the phenyl hydroxyalkyl ether arranged, particularly because many microorganisms are killed antibacterial and fungicidal effect and the well-known chemical compound that is referred to as phenoxyethanol.

Other antimicrobial is suitable for being incorporated in the article according to the invention equally.For example, useful material has 2,4,4 '-three chloro-2 '-xenol ether (triclosan), 1,6-two (4-chlorobenzene biguanide) hexane (chlorhexidine), 3,4,4 '-Amolden MCM 400, quaternary ammonium compound, Oleum Caryophylli, Oleum menthae, thyme oil, triethyl citrate, farnesol (3,7,11-trimethyl-2,6,10-12 carbon triolefin-1-alcohol), and active component of describing in following patent disclosure description or active component combination: DE-37 40186, DE-39 38 140, DE-42 04 321, DE-42 29 707, DE-43 09 372, DE-4411 664, DE-195 41 967, DE-195 43 695, DE-195 43 696, DE-195 47 160, DE-196 02 108, DE-196 02 110, DE-196 02 111, DE-196 31 003, DE-196 31004 and DE-196 34 019, and patent specification DE-42 29 737, DE-42 37 081, DE-4324 219, DE-44 29 467, DE-44 23 410 and DE-195 16 705.Also be preferably to use sodium bicarbonate.Similarly also can use antimicrobial polypeptide.

Suitable lightscreening agent active component is the material that absorbs the ultraviolet rays in UV-B and/or the ultraviolet light,long wave district.For example, suitable ultraviolet filter agent is 2,4,6-triaryl-1,3, the 5-triazine, at least one substituent group of aryl portability in either case wherein, preferred substituents is selected from hydroxyl, alkoxyl, methoxyl group particularly, alkoxy carbonyl group, particularly methoxycarbonyl group and ethoxycarbonyl and their mixture.Same suitable have p-aminobenzoate, cinnamate, benzophenone, camphor derivatives and the pigment that can stop ultraviolet rays, for example titanium dioxide, Talcum and zinc oxide.

Suitable ultraviolet filter material is any ultraviolet light,long wave and UV-B ray filtering material.That can mention has a following example:

Numbering	Material	CAS numbers (=acid)
			1	The 4-amino benzoic Acid	150-13-0
2	3-(4 '-trimethylamine) benzal thatch alkane-2-ketone methylsulfuric acid ester	52793-97-2
			3	3,3,5-trimethylcyclohexyl salicylate (homosaligenin)	118-56-9
4	2-hydroxyl-4-methoxyl group-benzophenone (oxybenzone)	131-57-7
			5	2-Phenylbenzimidazole-5-sulfonic acid and potassium salt, sodium salt and triethanolamine salt	27503-81-7
6	3,3 '-(1, the 4-phenylenedimethylidyne) two (7,7-dimethyl-2-oxo dicyclo [2.2.1] heptane-1-methane-sulfonic acid) and salt thereof	90457-82-2
			7	Poly-ethoxyethyl-4-two (poly-ethoxy) Aminobenzoate	113010-52-9
8	2-ethylhexyl 4-dimethylaminobenzoic acid ester	21245-02-3
			9	The 2-Ethylhexyl salicylate	118-60-5
10	2-isopentyl 4-Methoxycinnamate	71617-10-2
			11	2-ethylhexyl 4-Methoxycinnamate	5466-77-3
12	2-hydroxyl-4-methoxyl group benzophenone-5-sulfonic acid (sulisobenzone) and sodium salt thereof	4065-45-6

13	3-(4 '-sulfo-benzal) thatch alkane-2-ketone and salt thereof	58030-58-6
			14	3-benzal thatch alkane-2-ketone	16087-24-8
15	1-(4 '-isopropyl phenyl)-3-phenyl-propane-1, the 3-diketone	63260-25-9
			16	4-isopropyl phenyl salicylate	94134-93-7
17	3-imidazol-4 yl acrylic acid and ethyl ester thereof	104-98-3
			18	2-cyano-3,3-diphenyl ethyl acrylate	5232-99-5
19	2 '-ethylhexyl 2-cyano group-3,3-diphenylacrylate ester	6197-30-4
			20	Methyl 2-aminobenzoate or 5-methyl-2-(1-Methylethyl)-2-Aminobenzoate	134-09-8
21	Para-amino benzoic acid glyceride or 1-glyceryl 4-Aminobenzoate	136-44-7
			22	2,2 '-dihydroxy-4-methoxyl group benzophenone (dihydroxyphenyl ketone)	131-53-3
23	2-hydroxyl-4-methoxyl group-4-methyldiphenyl ketone (mexenone)	1641-17-4
			24	The triethanolamine Salicylate	2174-16-5
25	Dimethoxyphenyl glyoxalic acid or 3,4-Dimethoxyphenyl glyoxalic acid sodium	4732-70-1
			26	3-(4 '-sulfo-) benzal thatch alkane-2-ketone and salt thereof	56039-58-8
27	The 4-tert-butyl group-4 '-methoxy dibenzoyl methane	70356-09-1
			28	2,2 ', 4,4 '-tetrahydroxybenzophenone	131-55-5
29	2,2 '-di-2-ethylhexylphosphine oxide [6-(2H-benzotriazole-2-yl)-4-(1,1,3, the 3-tetramethyl butyl) phenol]	103597-45-1
			30	2,2 '-(1, the 4-phenylene) is two-1H-benzimidazole-4,6-disulfonic acid, sodium salt	180898-37-7
31	2, two [4-(2-ethyl hexyl oxy)-2-hydroxyl] phenyl-6-(4-methoxyl group-phenyl) of 4--(1,3,5) triazine	187393-00-6
			32	3-(4 '-methyl benzal) Camphora	36861-47-9
33	Poly-ethoxyethyl-4-two (poly-ethoxy) p-aminobenzoate	113010-52-9
			34	2,4 dihydroxy benzophenone	131-56-6
35	2,2 '-dihydroxy-4,4 '-dimethoxy-benzophenone-5,5 '-sodium disulfonate	3121-60-6

36	2-[4-(diethylamino)-2-hydroxy benzoyl]-benzoic acid hexyl ester	302776-68-7
			37	2-(2H-2-benzotriazole-2-yl)-4-methyl-6-[2-methyl-3-[1,3,3,3-tetramethyl-1-[(trimethyl silyl) the oxygen base]-the disiloxane base] propyl group]-phenol	155633-54-8
38	1,1-[(2,2 '-diformazan propoxyl group) carbonyl]-4,4-hexichol-1,3-butadiene	363602-15-7

Can preferably additionally contain according to cosmetic of the present invention and dermatosis goods and to stop ultravioletly, and can be selected from zinc oxide (ZnO), titanium oxide (TiO based on the insoluble or water-soluble slightly metal-oxide and/or the inorganic pigment of other metallic compound ₂), iron oxides is (as Fe ₂O ₃), Zirconium oxide (ZrO ₂), Si oxide (SiO ₂), Mn oxide (as MnO), aluminum oxide (Al ₂O ₃), cerium oxide is (as Ce ₂O ₃), the mixed oxide of respective metal and the mixture of this type oxide.

The inorganic pigment of this paper can be a coating form, promptly through surface treatment.For example, this surface treatment can comprise with itself known method form hydrophobic thin film on pigment, as described in DE-A-33 14 742

Suitable repellant active component is or to drive the particularly chemical compound of insecticide of some animal away from the human body expeling.For example, these comprise 2-ethyl-1, the 3-hexanediol, and N, toluamide between the N-diethyl, or the like.Stimulate blood for example essential oil to be arranged by the mobile suitable congested material of skin, for example Pinus mugo extract, Garden lavender extract, Herba Rosmarini Officinalis extract, Juniperus rigida Sieb.et Zucc. extract, Aesculus chinensis Bunge extract, birch leaf extract, Radix Glycyrrhizae flower extract, ethyl acetate, Camphora, menthol, Oleum menthae, Herba Rosmarini Officinalis extract, Eucalyptus oil, or the like.For example, the suitable keratolysis and the material of ceratoplasty have salicylic acid, mercaptan calcium acetate, mercaptan acetic acid and salt thereof, sulfur, or the like.For example, suitable dandruff removing agent active component has sulfur, sulfuration Polyethylene Glycol sorbitan mono-oleic acid ester, sulfuration Semen Ricini alcohol polyethoxylate, pyrithione zinc, pyrithione aluminum, or the like.For example, reducing skin irritant suitable antiinflammatory has allantoin, Bisabolol terpene alcohol, bisabolol, Flos Chrysanthemi extract, pantothenylol, or the like.

Can contain as making up and/or (and if also suitable also as adjuvant) at least a cosmetic or pharmaceutically acceptable polymer of active constituents of medicine according to make-up composition of the present invention, wherein said polymer is different from the polymer that can generate the used polyelectrolyte complex compound of the present invention.Normally, these comprise cation, both sexes and neutral polymer.

For example, suitable polymers has the polyquaternary ammonium salt cationic polymer of INCI name, as copolymer (Luviquat FC, Luviquat HM, LuviquatMS, the Luviquat﹠amp of vinylpyrrolidone/N-vinyl imidazole salt; Commat, Care), with dithyl sulfate quaternised N-vinylpyrrolidone/dimethylaminoethyl methacrylate copolymer (Luviquat PQ 11), N-ethylene caprolactam/N-vinylpyrrolidone/N-vinyl imidazole salt copolymer (Luviquat E Hold), cationic cellulose derivative (polyquaternary ammonium salt-4 and polyquaternary ammonium salt-10), acrylamide copolymer (polyquaternary ammonium salt-7) and chitosan.

Suitable cationic polymers (quaternised) also has Merquat (based on the muriatic polymer of dimethyldiallylammonium), Gafquat (reacting the quadripolymer that produces by polyvinylpyrrolidone and quaternary ammonium compound), polymer JR (hydroxyethyl-cellulose with cation group), and based on the cationic polymer of plant, as guar polymer, for example from the guar polymer of the Jaguar grade series of Rhodia (Luo Diya).

Other suitable polymers also has neutral polymer, polyvinylpyrrolidone for example, the copolymer of N-vinyl pyrrolidone and vinyl acetate and/or propionate, polysiloxanes, polyethylene caprolactam and other have N-vinylpyrrolidone copolymers, polymine and salt thereof, polyvinylamine and salt thereof, cellulose derivative, polyaspartic acid salts and derivant.For example, these comprise Luviflex 0 Swing (the saponified copolymer of polyvinyl acetate and polyalkylene glycol moiety, BASF).

Suitable polymers also has non-ionic water-soluble or water-dispersible polymer or oligomer, polyethylene caprolactam (as Luviskol 0 Plus (BASF)) for example, or polyvinylpyrrolidone and copolymer thereof, the copolymer that particularly has vinyl acetate, vinyl acetate (Luviskol 0 VA 37 (BASF)) for example, and for example based on the polyamide of methylene succinic acid and aliphatic diamine, for example, as described in the DE-A-4333238.

Suitable polymers also has both sexes or amphoteric ion polymer, octyl acrylamide/methyl methacrylate/tert-butyl group aminoethyl methacrylate/Hydroxypropyl methacrylate the copolymer that for example can trade name Amphomer (National Starch) obtains, and in German patent application DE39 29 973, DE21 50 557, DE28 17 369 and DE37 08 451 disclosed amphoteric ion polymer.Acrylamido oxypropyl trimethyl ammonium chloride/acrylic or methacrylic acid copolymer and alkali metal salt thereof and ammonium salt are preferred amphoteric ion polymers.Other suitable amphoteric ion polymer have can the commercial acquisition of trade name Amersette (AMERCHOL) the methylpropenyl ethyl in ammonium ester (methacroylethylbetaine)/methacrylate copolymer, and hydroxyethyl meth acrylate, methyl methacrylate, methacrylic acid N, N-dimethylaminoethyl and acrylic acid copolymer (Jordapon (D)).

Suitable polymers also has nonionic to contain the water solublity or the water-dispersible polymers of siloxanes, as polyether silicone, and for example Tegopren 0 (Goldschmidt) or Besi﹠amp; Commat (Wacker).

Preparation matrix optimization according to pharmaceutical composition of the present invention comprises pharmaceutically acceptable adjuvant.Pharmaceutically acceptable adjuvant is because of use well-known adjuvant in pharmaceutical field, Food technology and association area, those listed adjuvant in relevant pharmacopeia (as DAB Ph.Eur.BP NF) particularly, with and the character adjuvant that do not hinder the physiology to use.

Suitable adjuvant can be: lubricant, wetting agent, emulsifying agent and suspending agent, antiseptic, antioxidant, counter-stimulus, chelating agen, emulsion stabilizer, film former, gelatinizing agent, odor masking agent, resin, hydrocolloid, solvent, solubilizing agent, nertralizer, penetration enhancer, pigment agent, quaternary ammonium compound, fatting agent and superfatting agent, unguentum, cream or oily matter, silicone derivative, stabilizing agent, antibacterial, propellant, desiccant, opacifier, thickening agent, wax, softening agent, white oil.In this respect, prescription is benchmark with the expertise, for example, as Fiedler, H.P (Lexikon der Hilfsstoffe f ü rPharmazie, the adjuvant dictionary of Kosmetik und angrenzende Gebiete[pharmacy, cosmetic and association area], the 4th edition, Aulendorf:ECV-Editio-Kantor-Verlag, 1996) knowledge that is provided in.

In order to prepare according to dermatosis compositions of the present invention, available suitable adjuvant (excipient) mixes or the dilution active component.Excipient can be solid, semisolid or the liquid charging stock of the carrier, carrier or the medium that can be used as active component.If expectation can add other adjuvant in the manner known to persons skilled in the art.In addition, polymer and dispersion are suitable for as adjuvant in pharmacy, preferably as or be used for the coating or the binding agent of solid pharmaceutical dosage formulation.They can also be used for cream and as tablet coating and tablet binder.

According to embodiment preferred, compositions of the present invention is a skin cleansing compositions.

Preferred skin cleansing compositions is the liquid soap of gluey concentration, for example transparent soap, high-grade fancy soap, deodorant soap, cream soap, baby's soap, skin-protection soap, abrasive soap and synthetic detergent, pasty state soap (pastysoaps), soft soap and cleaning paste, exfoliation soap (exfoliation soaps), preserve moisture and wipe paper, liquid scrubbing thing, shower and bathing goods, for example washing liquid, shower lotion, shower glue, bath foam, oil bath and scrub goods, shaving foam glue, washing liquid and cream.

According to another preferred embodiment, compositions of the present invention is make-up composition, the manicure compositions of nursing and protection skin and hair or the goods that are used for ornamental cosmetic.

For example, the suitable skin make-up composition is facial cosmetic water (tonic), facial film, deodorizer and other astringent.For example, the compositions that is used for decorative cosmetic product having comprises: concealer (concealingstick), stage cosmetics (stage makeup), mascara and eye shadow cream, lipstick, cosmetic pencil, eyeliner, kermes, face powder and eyebrow pencil.

In addition, peptide sequence (i) can be used for the subsides of pore cleaning nose, anti-acne composition, repellant, Shave composition, behind the care composition before and after shaving, Exposure to Sunlight among care composition, Depilatory composition, hair dye, privates care composition, foodcare compositions, the Baby Care compositions.

Particularly, skin care compositions according to the present invention be water-in-oil type or oil-in-water type protective skin cream, day cream and night frost, eye cream, facial cream, crease-proof cream, sunscreen cream, moisturiser, fair complexion cream, self-service U.S. black frost, vitamin cream, skin care liquid, conditioning liquid and moisture retention liquid.

Skin cosmetic and dermatosis compositions display based on above-mentioned polyelectrolyte complex compound go out superior effect.Wherein, polymer helps preserving moisture and conditioning skin, and improves the skin feel.Polymer also can be used as the thickening agent of preparation.By adding, in some preparation, can realize the improvement that the skin adaptability is considerable according to polymer of the present invention.

Skin is made up and the dermatosis compositions preferably comprises at least a about by weight 0.001% to 30%, preferred about by weight 0.01% to 20%, the extremely preferred peptide sequence (i) of about by weight 0.1% to 12% weight based on composition total weight.

Especially, compare, have the performance of the holdup time of improving the uv absorption composition based on the photoprotection compositions of peptide sequence (i) with the adjuvant such as the polyvinylpyrrolidone of routine.

According to the use field, compositions according to the present invention is used with the form that is suitable for skin protection, for example, and as cream, foam, gel, stick (stick), mousse, emulsion, fog-like body (atomizing pump or contain the spray of propellant) or lotion.

Except that peptide sequence (i) and suitable carriers, the skin cosmetic product also can comprise other active component and the conventional adjuvant in the aforesaid cosmetics for skin.They preferably include emulsifying agent, antiseptic, aromatic oil, cosmetic active component (for example phytantriol, vitamin A, vitamin E and vitamin C, retinol, Bisabolol terpene alcohol, pantothenylol), light protective agent, brightening agent, coloring agent, stain, tanning agent, collagen, protein hydrolysate, stabilizing agent, pH regulator agent, dyestuff, salt, thickening agent, gelatinizing agent, concentration regulator, silicone, humidizer, fatting agent and other conventional additive.

Skin is made up and the preferred lubricant component of dermatosis compositions is above-mentioned mineral oil and artificial oil, for example paraffin, silicone oil and more than the aliphatic hydrocarbon of 8 carbon atoms; Vegetable and animals oils, for example Oleum helianthi, coconut oil, American Avocado Tree oil, olive oil, lanoline or wax; Fatty acid or fatty acid ester, the triglyceride of C6-C30 fatty acid for example, wax ester, for example Simmondsia chinensis oil, aliphatic alcohol, vaseline, hydrogenated lanolin and acetylated lanolin, and their mixture.

If the establishment special performances also can be mixed with conventional polymer according to peptide sequence of the present invention (i).

In order to create some performance, for example improve the water-resistance and/or the combination of sense of touch, spreading property and active component and adjuvant (for example pigment), described skin cosmetic and skin goods can also comprise the conditioning material based on silicone compounds in addition.

For example, suitable silicone compounds has poly-alkylsiloxane, poly-aryl siloxanes, poly-aryl alkyl siloxanes, polyether siloxane or silicone resin.

Cosmetic or dermatosis goods can prepare according to conventional method well known by persons skilled in the art.

Preferably, cosmetic and dermatosis compositions are Emulsion form, particularly water-in-oil type (W/O) or oil-in-water type (O/W) Emulsion.

But, can also select other dosage form, example gel agent, oil preparation, oleogel, multiple Emulsion be W/O/W or the form of O/W/O type Emulsion, anhydrous unguentum or paste substrate for example, or the like.The dosage form of emulsifier-free also is superior embodiment, for example water dispersant (hydrodispersion), hydrogel or Pickering Emulsion.

Emulsion prepares by known method.Except that at least a peptide sequence (i), Emulsion comprises conventional component usually, particularly fatty acid triglyceride, fatty acid, lanoline and derivant thereof, natural or synthetic oil or wax of aliphatic alcohol, fatty acid ester for example, and be present in emulsifying agent among the water.For example, Schrader, the ultimate principle and the preparation of Grundlagen und Rezepturen der Kosmetika[cosmetics], H ü thig Buch Verlag, Heidelberg, second edition, 1989, third part clearly is incorporated herein by reference hereby) additive that is specific to the Emulsion kind and the preparation of suitable Emulsion described.

Usually comprise by suitable emulsifier system as the suitable Emulsion of water-in-oil emulsion (as protective skin cream etc.) and to carry out emulsive water in mutually at oil phase or fat.For water is provided, can use polyelectrolyte complex compound.

Being present in the Emulsion fat fat constituent among mutually preferably has: Hydrocarbon oil preparation, for example the microwax solution in paraffin oil, purcellin oil, perhydro-squalene and these oil; Animal or plant oil, for example Semen pruni armeniacae oil, American Avocado Tree oil, Caulis et folium euphorbiae milii (calophylum) oil, lanoline and derivant thereof, Oleum Ricini, Oleum sesami, olive oil, Simmondsia chinensis oil, cream wood fruit (karit é) oil, breast sour jujube Channa argus (hoplostethus) oil; Normal pressure down about 250 ℃ and distillation end point of distillation starting point is 410 ℃ a mineral oil, for example vaseline oil; Saturated or unsaturated fatty acid ester, alkyl myristinate for example, as isopropyl myristate, butyl myristate or myristic acid spermaceti alcohol ester, hexadecyl stearate, ethyl palmitate or isopropyl palmitate, Trivent OCG or tricaprin and cetyl ricinoleate.

Be dissolved in other oily silicone oil fatty also can comprising mutually, for example dimethicone, toluene polysiloxanes and silicone glycol copolymer, fatty acid and aliphatic alcohol.

Except that peptide sequence (i), also might use wax, for example Brazil wax, candelilla wax, Cera Flava, microwax, ceresine, and the oleate of Ca, Mg and Al, myristate, linoleate and stearate.

In addition, can be the oil-in-water emulsion form according to Emulsion of the present invention.Such Emulsion comprises oil phase usually, makes oil phase at aqueous phase stable emulsifying agent and water, and it exists with dense thick form usually.Suitable emulsifying agent is oil-in-water emulsifiers preferably, for example the glyceride of polyglycerin ester, sorbitan ester or partial esterification.

According to another preferred embodiment, compositions of the present invention is shower lotion, hair washing preparation or bath goods.

Such preparation comprises at least a peptide sequence (i) and as the conventional anion surfactant of surface of base activating agent and as the both sexes and/or the nonionic surfactant of cosurfactant.Active component that other is suitable and/or adjuvant are selected from lipid, aromatic oil, dyestuff, organic acid, antiseptic and antioxidant and thickening agent/gelatinizing agent, skin conditioning agent and humidizer usually.

These preparations preferably comprise about by weight 2% to 50%, preferred about by weight 5% to 40%, preferred especially about by weight 8% to 30% the surfactant based on total formulation weight.

In washing, shower and bathing goods, might use all aniones, neutrality, both sexes or cationic surfactant among the compositions that is generally used for cleaning health.

For example; suitable anion surfactant has alkyl sulfate, alkyl ether sulfate, alkylsulfonate, alkylaryl sulfonates, alkyl succinate, alkyl sulfo succinate, N-alkoxyl sarcosinate, acyl taurine salt, acyl-hydroxyethyl sulfonate, alkylphosphonic, alkyl ether phosphate, alkyl ether carboxy acid salt, alpha-alkene sulfonate; particularly alkali metal salt and alkali salt are as sodium salt, potassium salt, magnesium salt, calcium salt, ammonium salt and triethanolamine salt.Alkyl ether sulfate, alkyl ether phosphate and alkyl ether carboxy acid salt can have 1 to 10 oxirane or expoxy propane unit in molecule, preferred 1 to 3 ethylene oxide unit(s).

For example, these comprise sodium lauryl sulphate, tauryl ammonium sulfate, dodecyl ether sodium sulfate, ammonium dodecyl ether sulfate, sarcosyl, oily octenyl succinate sodium, dodecyl 2-Sulfosuccinic acid ammonium, dodecylbenzene sodium sulfonate, triethanolamine dodecylbenzene sodium sulfonate.

For example, suitable amphoteric surfactant has alkyl betaine, alkyl amino CAB, alkyl sulfobetaines, p dialkylaminobenzoic acid salt, alkyl carboxyl Glycinates, alkyl both sexes acetate or both sexes propionate, alkyl both sexes diacetin or both sexes dipropionate.

For example, can use coco dimethyl sulfopropyl betaine (cocodimethylsulfopropylbetaine), empgen BB, cocamidopropyl betaine (cocamidopropylbetaine) or cocos nucifera oil both sexes sodium propionate (sodiumcocamphopropionate).

For example, suitable nonionic surfactant is the product that has aliphatic alcohol or alkyl phenol and the oxirane and/or the expoxy propane of 6 to 20 carbon atoms in the straight or branched hydrocarbon chain.The amount of epoxyalkane is about 6 to 60 moles of every mol of alcohol.In addition, fatty acid ester, ethoxylated fatty acid amide, alkyl poly glucoside or the sorbitan ether-ether of alkyl amine oxide, an alkyl alkanolamine or dialkyl group alkanolamine, Polyethylene Glycol are fit to.

In addition, washing, shower and bathing goods can comprise conventional cationic surfactant, and quaternary ammonium compound for example is as hexadecyltrimethylammonium chloride.

In addition, shower lotion/hair washing preparation can comprise thickening agent, for example sodium chloride, PEG-55, propylene glycol oleate, PEG-120, methyl glucoside dioleate etc., and antiseptic, other active component and adjuvant and water.

According to another preferred embodiment, compositions of the present invention is a Hiar treatment compositions.

The peptide sequence (i) that preferably comprises at least a about by weight 0.01% to 30% based on composition total weight, preferred about by weight amount of 0.5% to 20% according to Hiar treatment compositions of the present invention.

Preferably, Hiar treatment compositions according to the present invention is following form: typing foamed glue, hair mousse, hair jelly, shampoo, hair spray, hair foamed glue, hydrojet agent (spitzenfluid) are used for nertralizer, hair dye and brightening agent or the hot oil processing agent of lasting hair-waving.According to the use field, the hair cosmetic product can be used as (aerosol) spray, (aerosol) foamed glue, hair jelly, hair jelly spray, hair ointment, hair conditioner or pomade and uses.The hair spray of this paper comprises the pump formula spray of aerosol spray agent and no propelling gas.The hair foamed glue comprises the pump formula foamed glue of aerosol foam glue and no propelling gas.Hair spray and hair foamed glue preferably comprise water solublity or water-dispersible composition highlightedly or exclusively.If it is water-dispersible being used for the chemical compound of the present invention of agent and hair foamed glue, they are that the form of a water differential prose style free from parallelism of 1 to 350nm, preferred 1 to 250nm is used with particle diameter usually.The solid content of these goods is usually in about scope of 0.5% to 20% by weight herein.Usually these differential prose style free from parallelisms do not need emulsifying agent or surfactant to its stabilisation in addition.

In preferred embodiments, hair make-up preparation according to the present invention comprises: a) be 0.01% to 30% at least a peptide sequence (i) by weight; B) be 20% to 99.95% water and/or alcohol by weight; C) be 0 to 50% at least a gaseous propellant by weight; D) be 0 to 5% at least a emulsifying agent by weight; E) be 0 to 3% at least a thickening agent by weight; Go up by weight other component to 25%.

Alcohol refers to the alcohol of all routines in the cosmetics, as ethanol, isopropyl alcohol, normal propyl alcohol.

Other component refers to additive conventional in the cosmetics, and for example propellant, defoamer, interfacial activity chemical compound are surfactant, emulsifying agent, foam agent and solubilizing agent.The interfacial activity chemical compound that uses can be anion, cation, both sexes or neutral compound.For example, other conventional component also can be an antiseptic, aromatic oil, opacifier, active component, ultraviolet filter agent, care substance, for example pantothenylol, collagen, vitamin, protein hydrolysate, α and β hydroxy carboxylic acid, stabilizing agent, the pH regulator agent, dyestuff, viscosity modifier, gelatinizing agent, salt, humidizer, fatting agent, chelating agent and other conventional additive.

If create very special performances, these also comprise all known hair styles and the conditioner polymer in cosmetic field that can unite use with peptide sequence of the present invention (i).

For example, suitable conventional hair cosmetic polymer is above-mentioned cationic polymer, anionic polymer, neutral polymer, non-ionic polymers and amphiphilic polymers, at this it is incorporated herein by reference.

In order to create some performance, goods also can comprise the conditioning material based on silicone compounds in addition.For example, suitable silicone compounds has poly-alkylsiloxane, poly-aryl siloxanes, poly-aryl alkyl siloxanes, polyether silicone, silicone resin or dimethicone copolyol (CTFA) and amido functional group silicone compounds, for example amino polydimethylsiloxane (CTFA).

Polymer according to the present invention is particularly suitable as setting agent, particularly hair spray (the pump formula spray of aerosol spray agent and no propelling gas) and the hair foamed glue (the pump formula foamed glue of aerosol foam glue and no propelling gas) in the hair style goods.

In preferred embodiments, the spray goods comprise: a) be 0.01% to 30% at least a peptide sequence (i) by weight; B) be 20% to 99.9% water and/or alcohol by weight; C) be 0 to 70% at least a propellant by weight; D) be other composition of 0 to 20% by weight.

Propellant is the propellant that is generally used for hair spray or aerosol foam glue.Preferably propane/butane mix, pentane, dimethyl ether, 1, the 1-Difluoroethane (HFC-152 a), carbon dioxide, nitrogen or compressed air.

The preparation that is used for aerosol hair foamed glue of the present invention preferably includes: a) be 0.01% to 30% at least a peptide sequence (i) by weight; B) be 55% to 99.8% water and/or alcohol by weight; C) be 5% to 20% propellant by weight; D) be 0.1% to 5% emulsifying agent by weight; E) be other component of 0 to 10% by weight.

Employed emulsifying agent is all emulsifying agents that are generally used for the hair foamed glue.Suitable emulsifying agent is nonionic, cation or anion or amphoteric emulsifier.

The example of nonionic emulsifier (INCI name) has lauryl alcohol (laureth), as lauryl alcohol-4; Spermaceti alcohol ether (ceteth) is as spermaceti alcohol ether-1; The Polyethylene Glycol cetyl ether, ceteareth (ceteareth) is as ceteareth-25; Polyethylene glycol fatty acid glyceride, hydroxylated lecithin, fatty acid lactoyl ester, alkyl poly glucoside.

The example of cationic emulsifier has cetyl dimethyl-2-ethoxy Ammonium biphosphate, cetyl trimethyl ammonium chloride, cetyl trimethylammonium bromide, cocos nucifera oil trimethyl sulfate methyl ammonium, quaternary ammonium salt-1 to x (INCI).

For example; anion emulsifier can be selected from alkyl sulfate, alkyl ether sulfate, alkylsulfonate, alkylaryl sulfonates, alkyl succinate, alkyl sulfo succinate, N-alkoxyl sarcosinate, acyl taurine salt, acyl-hydroxyethyl sulfonate, alkylphosphonic, alkyl ether phosphate, alkyl ether carboxy acid salt, alpha-alkene sulfonate; particularly alkali metal salt and alkali salt are as sodium salt, potassium salt, magnesium salt, calcium salt, ammonium salt and triethanolamine salt.Alkyl ether sulfate, alkyl ether phosphate and alkyl ether carboxy acid salt can have 1 to 10 oxirane or expoxy propane unit in molecule, preferred 1 to 3 ethylene oxide unit(s).

For example, the suitable goods of the present invention that are applicable to hair style glue can have following compositions: a) be 0.01% to 30% at least a peptide sequence (i) by weight; B) be 80% to 99.85% water and/or alcohol by weight; C) be 0 to 3% by weight, preferably be 0.05% to 2% gelatinizing agent by weight; D) be other component of 0 to 20% by weight.

Usually, peptide sequence used according to the invention (i) has " thickening automatically " effect, means when the preparation gel, can exempt the use of gelatinizing agent as a rule.Yet for special flow degeneration or other application performance of creating gel, the use of gelatinizing agent may be best.Available gelatinizing agent has all conventional gelatinizing agents in the cosmetics.These comprise lightly crosslinked polyacrylic acid, carbomer (INCI) for example, cellulose derivative, as hydroxypropyl cellulose, hydroxyethyl-cellulose, cation modified cellulose, polysaccharide such as xanthan gum, caprylic/capric triglyceride, sodium acrylate copolymer, polyquaternium-32 (with) paraffin oil (INCI), sodium acrylate copolymer (with) paraffin oil (with) PPG-1 polyoxyethylene tridecyl-6 (PPG-1 trideceth-6), acrylamido oxypropyl trimethyl ammonium chloride/acrylamide copolymer, stearic alcohol ether-10 (steareth), allyl ether, acrylic copolymer, polyquaternary ammonium salt-37 (with) paraffin oil (with) PPG-1 polyoxyethylene tridecyl ether-6, polyquaternary ammonium salt 37 (with) propylene glycol dicaprate/dicaprylate (with) PPG-1 polyoxyethylene tridecyl ether-6, polyquaternary ammonium salt-7, polyquaternary ammonium salt-44.

Can be used as conditioner in the cosmetic product according to peptide sequence of the present invention (i).

The goods that comprise peptide sequence of the present invention (i) preferably are used among the shampoo preparation as setting agent and/or conditioner.Preferred shampoo preparation comprises: a) be 0.01% to 30% at least a peptide sequence (i) by weight; B) be 20% to 94.95% water by weight; C) be 5% to 50% surfactant by weight; C) be other conditioner of 0 to 5% by weight; D) be other cosmetic components of 0 to 10% by weight.

In the shampoo preparation, might use all aniones, neutrality, both sexes or the cationic surfactant that are generally used for shampoo.

For example; suitable anion surfactant has alkyl sulfate, alkyl ether sulfate, alkylsulfonate, alkylaryl sulfonates, alkyl succinate, alkyl sulfo succinate, N-alkoxyl sarcosinate, acyl taurine salt, acyl-hydroxyethyl sulfonate, alkylphosphonic, alkyl ether phosphate, alkyl ether carboxy acid salt, alpha-alkene sulfonate; particularly alkali metal salt and alkali salt are as sodium salt, potassium salt, magnesium salt, calcium salt, ammonium salt and triethanolamine salt.Alkyl ether sulfate, alkyl ether phosphate and alkyl ether carboxy acid salt can have 1 to 10 oxirane or expoxy propane unit in molecule, preferred 1 to 3 ethylene oxide unit(s)

What for example, be fit to has sodium lauryl sulphate, ammonium lauryl sulfate, dodecyl ether sodium sulfate, ammonium dodecyl ether sulfate, sarcosyl, oily octenyl succinate sodium, dodecyl 2-Sulfosuccinic acid ammonium, dodecylbenzene sodium sulfonate, a triethanolamine dodecylbenzene sodium sulfonate.

For example, can use coco dimethyl sulfopropyl betaine, empgen BB, cocamidopropyl betaine or cocos nucifera oil both sexes sodium propionate.

For example, suitable nonionic surfactant is the product that has aliphatic alcohol or alkyl phenol and the oxirane and/or the expoxy propane of 6 to 20 carbon atoms in the straight or branched hydrocarbon chain.The amount of epoxyalkane is about 6 to 60 moles of every mol of alcohol.In addition, fatty acid ester, alkyl poly glucoside or the sorbitan ether-ether of alkyl amine oxide, an alkyl alkanolamine or dialkyl group alkanolamine, Polyethylene Glycol are fit to.

In addition, the shampoo preparation can comprise conventional cationic surfactant, and quaternary ammonium compound for example is as hexadecyltrimethylammonium chloride.

In the shampoo preparation, conventional conditioner can be united use with peptide sequence (i), to realize some effect.

For example, these comprise copolymer (Luviquat FC, Luviquat HM, Luviquat MS, the Luviquat﹠amp of cationic polymer, the particularly vinylpyrrolidone/N-vinyl imidazole salt of the polyquaternary ammonium salt that the above-mentioned INCI of having names; Commat, Care), with dithyl sulfate quaternised N-vinylpyrrolidone/dimethylaminoethyl methacrylate copolymer (Luviquat D PQ 11), N-ethylene caprolactam/N-vinylpyrrolidone/N-vinyl imidazole salt copolymer (Luviquat D Hold), cationic cellulose derivative (polyquaternary ammonium salt-4 and-10), acrylamide copolymer (polyquaternary ammonium salt-7).In addition, can use protein hydrolysate and, for example poly-alkylsiloxane, poly-aryl siloxanes, poly-aryl alkyl siloxanes, polyether silicone or silicone resin based on the adjusting material of silicone compounds.Other suitable silicone compounds has dimethicone copolyol (CTFA) and amido functional group silicone compounds, for example amino polydimethylsiloxane (CTFA).In addition, can use cationic guar derivative, for example guar gum hydroxypropyl-trimethyl ammonium chloride (INCI).

The invention still further relates to keratin-binding effector molecules, comprising:

(i) at least a have the peptide sequence of binding affinity to keratin,

The (ii) effector molecule that is connected with peptide sequence (i) and non-natural.

Suitable peptide sequence (i) as mentioned above.

I. peptide sequence (domain B)

Ii. peptide sequence (domain C)

Iii. compare with (a), go up to the adorned peptide sequence of 70% aminoacid.

Iv. compare with (b), go up to the adorned peptide sequence of 70% aminoacid.

Correspondingly, for the application of house pet aspect, preferred peptide sequence (i) is that corresponding keratin (for example Canis animals keratin or felid keratin) is had the sequence of special high-affinity.

Preferred especially following sequence area:

Domain B (KBD-B): among the peptide sequence SEQ ID NO:1 the 2193rd to 2448;

Domain B (KBD-B): among the peptide sequence SEQ ID NO:1 the 2209th to 2448;

Domain C (KBD-C): among the peptide sequence SEQ ID NO:1 the 2606th to 2871;

Domain C (KBD-C): among the peptide sequence SEQ ID NO:1 the 2616th to 2871;

Domain C (KBD-C): among the peptide sequence SEQ ID NO:1 the 2616th to 2811;

Domain C (KBD-C): among the peptide sequence SEQ ID NO:1 the 2606th to 2871.

Fig. 2 has shown the comparison of keratin binding molecule.

Effector molecule (ii)

Effector molecule (ii) is meant the molecule with specific measurable effect hereinafter.They can be protein molecule such as enzyme, perhaps also can be the molecule of non-proteinogen such as the chemical compound of dyestuff, opacifier, vitamin, provitamin, antioxidant and fatty acid, conditioner or metal ion.

Among the protein effector molecule, preferably surely belong to enzyme and antibody.

Among enzyme, preferred following action effect molecule (ii): oxidase, peroxidase, protease, glucanase, mutant enzyme, tryrosinase, laccase, melts combine enzyme, lactoperoxidase, lysozyme, starch glycosidase, glucoseoxidase, superoxide dismutase, photolyase, T4 Cobra venom endonuclease, catalase, thioredoxin, thioredoxin reductase.

Following be preferred action effect molecule non-enzymatic activity protein effector molecule (ii) (ii): antibacterial peptide, silk protein, hydrophobin, collagen (collaten), carotenoid is conjugated protein, heavy metal is conjugated protein, OBP.

Be suitable as equally very much the protein effector molecule (ii) be the protein hydrolysate in plant and animal source, for example protein hydrolysate in marine products source or silk hydrolysate.

The nonprotein effector molecule (ii) among, preferably surely belong to dyestuff, for example food coloring, impermanency dyestuff or chemically-reactive dyes or oxidation dye.With regard to oxidation dye, preferably with a kind of component and keratin-binding polypeptides sequence (i) coupling (ii) of action effect molecule, subsequently at (after being attached on the hair) on the action site and the second dye component oxidative coupling.Also, implement the coupling of colour component by oxidation dye preferably being connected to peptide sequence (i) before.

Also can preferably chemically-reactive dyes action effect molecule a kind of component (ii) be connected on the keratin-binding polypeptides sequence (i), it is attached on the hair.In addition, might be in decorative cosmetic product having use the action effect molecule (ii) to be connected to such dyestuff on the keratin-binding polypeptides sequence (i) by being incorporated into fingernail or skin.

The suitable dye that is used for molecule of the present invention is all conventional hair dyess.According to the cosmetics handbook, suitable dyestuff is known for the technical staff, Schrader for example, Grundlagen undRezepturen " der Kosmetika, H ü thig Verlag, Heidelberg, 1989, ISBN3-7785-1491-1 ".

Particularly advantageous dyestuff is the dyestuff of listing in following table.Color index number (CIN) is selected from " RoweColour Index, the 3rd edition, Society of Dyers and Colourists, Bradford, England, 1971 ".

Chemical name or other title	?CIN	Color
			Phthalocyanine green	?10006	Green
Acid green 1	?10020	Green
			2,4-dinitro hydroxyl naphthalene-7-sulfonic acid	?10316	Yellow
Pigment yellow 1	?11680	Yellow
			Pigment yellow 3	?11710	Yellow
Pigment orange
	1	?11725	Orange
2,4-dihydroxy diphenyl diimide				?11920	Orange
	Solvent red 3	?12010	Red
1-(2 '-chloro-4 '-nitro-1 '-phenylazo)-2 hydroxy naphthalene				?12085	Red
	Pigment red 3	?12120	Red
Solvent red; Tonyred; Oil red G				?12150	Red

Pigment red 112	12370	Red
			Paratonere 7	12420	Red
Pigment brown 1	12480	Palm fibre
			4-(2 '-methoxyl group-5 '-sulfo group lignocaine-1 '-phenylazo)-3-hydroxyl-5 "-chloro-2 ", 4 "-dimethoxy-2-naphthanilide	12490	Red
Disperse yellow 16	12700	Yellow
			1-(4-sulfo--1-phenylazo)-4-aminobenzene-5-sulfonic acid	13015	Yellow
2,4-dihydroxy diphenyl diimide-4 '-sulfonic acid	14270	Orange
			2-(2,4-xylene azoic base-5-sulfo group)-1-hydroxyl naphthalene-4-sulfonic acid	14700	Red
2-(4-sulfo--1-naphthylazo)-Neville acid	14720	Red
			2-(6-sulfo--2,4-xylene azoic)-1-naphthol-5-sulfonic acid	14815	Red
1-(4 '-sulfophenyl azo)-2 hydroxy naphthalene	15510	Orange
			1-(2-sulfo--4-chloro-5-carboxyl-1-phenylazo)-2 hydroxy naphthalene	15525	Red
1-(3-methylbenzene azo-4-sulfo group)-2 hydroxy naphthalene	15580	Red
			1-(4 ', (8 ')-sulfonic acid benzeneazo)-2 hydroxy naphthalene	15620	Red
2-hydroxyl-1,2 '-azonaphthalene-1 '-sulfonic acid	15630	Red
			3-hydroxyl-4-phenylazo-2-naphthalene-carboxylic acid	15800	Red
1-(2-sulfo--4-methyl isophthalic acid-phenylazo)-2-naphthalene-carboxylic acid	15850	Red
			1-(2-sulfo--4-methyl-5-chloro-1-phenylazo)-2 hydroxy naphthalene-3-carboxylic acid	15865	Red
1-(2-sulfo--1-naphthylazo)-2 hydroxy naphthalene-3-carboxylic acid	15880	Red
			1-(3-sulfo--1-phenylazo)-beta naphthal-6-sulfonic acid	15980	Orange
1-(4-sulfo--1-phenylazo)-beta naphthal-6-sulfonic acid	15985	Yellow
			Lure red	16035	Red
1-(4-sulfo--1-naphthylazo)-beta naphthal-3, the 6-disulfonic acid	16185	Red
			Acid orange 10	16230	Orange
1-(4-sulfo--1-naphthylazo)-beta naphthal-6, the 8-disulfonic acid	16255	Red
			1-(4-sulfo--1-naphthylazo)-beta naphthal-3,6, the 8-trisulfonic acid	16290	Red

8-amino-2-benzeneazo-1-naphthols-3, the 6-disulfonic acid	?17200	Red
			Azogeramine	?18050	Red
Azogeramine 55	?18130	Red
			Indian yellow 121	?18690	Yellow
Acid red 18 0	?18736	Red
			Acid yellow 11	?18820	Yellow
Indian yellow 17	?18965	Yellow
			4-(4-sulfo--1-benzeneazo)-1-(4-sulfophenyl)-5-hydroxypyrazoles quinoline ketone-3-carboxylic acid	?19140	Yellow
Pigment yellow 16	?20040	Yellow
			2,6-(4 '-sulfo--2 ", 4 "-dimethyl) two benzeneazos)-1, the 3-hydroquinone	?20170	Orange
Acid black 1	?20470	Black
			Pigment yellow 13	?21100	Yellow
Pigment yellow 83	?21108	Yellow
			Solvent yellow	?21230	Yellow
Azogeramine 63	?24790	Red
			Xylene Red 73	?27290	Red
2-[4 '-(4 "-sulfo--1 "-benzeneazo)-7 '-sulfo--1 '-naphthylazo]-1-hydroxyl-7-amino naphthalenes-3, the 6-disulfonic acid	?27755	Black
			4 '-[4 "-sulfo--1 "-benzeneazo)-7 '-sulfo--1 '-naphthylazo]-1-hydroxyl-8-acetyl-amino naphthalenes-3, the 5-disulfonic acid	?28440	Black
Direct orange 34,39,44,46,60	?40215	Orange
			Edible yellow	?40800	Orange
Trans-β-apo--8 '-carotenal (C30)	?40820	Orange
			Trans-apo--8 '-daucic acid (C30) ethyl ester	?40820	Orange
Canthaxanthin	?40850	Orange
			Blue VRS	?42045	Blue

2,4-two sulfur-5-hydroxyl-4 ', 4 "-two (diethyl amino) triphenylcarbinol	42051	Blue
			The 4-[(4-N-ethyl-to sulfophenyl (amino) phenyl-(4-hydroxyl-2-sulfophenyl) (methylene-1-(N-ethyl-N-is to sulfophenyl)-2,5-cyclohexyl diimine]	42053	Green
Acid Blue 7	42080	Blue
			(the N-ethyl-to the sulphur benzylamine) phenyl-(2-sulfophenyl) methylene-(N-ethyl-N-is to sulfophenyl)-δ 2,5-cyclohexyl diimine	42090	Blue
Acid green 9	42100	Green
			Diethyl two sulfur benzyls two-4-amino-2-chloro two-2-methyl fuchsonimmonium	42170	Green
Basis purple 14	42510	Purple
			Basis purple 2	42520	Purple
2 '-methyl-4 '-(sulfophenyl between N-ethyl-N-) amino-4 "-(N-diethyl) amino-2-methyl-N-ethyl-N-between sulphur benzene fuchsonimmonium	42735	Blue
			4 '-(N-dimethyl) amino-4 "-(N-phenyl) amino naphthalenes-N-dimethyl fuchsonimmonium	44045	Blue
2-hydroxyl-3,6-two sulfur-4,4 '-two dimethylamino naphthalene fuchsonimmonium	44090	Green
			Xylene Red 52	45100	Red
3-(2 '-amino toluene)-6-(2 '-methyl-4 '-sulfur phenylamino)-9-(2 "-carboxyl benzene) xanthene Salt	45190	Purple
			Xylene Red 50	45220	Red
Phenyl-2-oxygen base fluorone-2-carboxylic acid	45350	Yellow
			4, the 5-dibromofluorescein	45370	Orange
2,4,5, the 7-eosin	45380	Red
			Solvent dye	45396	Orange
Xylene Red 98	45405	Red
			3 ', 4 ', 5 ', 6 '-tetrachloro-2,4,5,7-eosin	45410	Red

4, the 5-diiodofluorescein	?45425	Red
			2,4,5, the 7-tetraiodofluorescein	?45430	Red
Quinophthalone (Quinophthalone)	?47000	Yellow
			The quinophthalone disulfonic acid	?47005	Yellow
Acid violet 50	?50325	Purple
			Acid black 2	?50420	Black
Pigment Violet 23	?51319	Purple
			1,2-dihydroxyanthraquinone, calcium-aluminum complex	?58000	Red
3-hydroxyl pyrene-5,8,10-sulfonic acid	?59040	Green
			1-hydroxyl-4-N-phenyl amino anthraquinone	?60724	Purple
1-hydroxyl-4-(4 '-aminomethyl phenyl amino) anthraquinone	?60725	Purple
			Acid violet 23	?60730	Purple
1,4-two (4 '-aminomethyl phenyl amino) anthraquinone	?61565	Green
			1, two (adjacent sulfo group-para-totuidine base) anthraquinones of 4-	?61570	Green
Acid blue 80	?61585	Blue
			Acid blue 62	?62045	Blue
N, N '-dihydro-1,2,1 ', 2 '-anthraquinone azine	?69800	Blue
			Vat blue 6; Alizarol saphirol 64	?69825	Blue
Urn orange 7	?71105	Orange
			Indigo	?73000	Blue
Indigo disulfonic acid	?73015	Blue
			4,4 '-dimethyl-6,6 '-dichloro thioindigo	?73360	Red
5,5 '-two chloro-7,7 '-dimethyl thioindigo	?73385	Purple
			Quinacridone violet 19	?73900	Purple
Pigment red 122	?73915	Red
			Pigment blue 16	?74100	Blue
Phthalocyanine	?74160	Blue

Sun blue 86	74180	Blue
			The chlorination phthalocyanine	74260	Green
Naturally yellow 6,19; Naturally red 1	75100	Yellow
			Annatto falls in annatto	75120	Orange
Lycopene	75125	Yellow
			Trans-α-, β-or gamma carotene	75130	Orange
The ketone of carotene and/or hydroxy derivatives	75135	Yellow
			Guanine or pearling agent	75170	In vain
1, two (the 4-hydroxy 3-methoxybenzene bases)-1 of 7-, 6-heptadiene-3,5-diketone	75300	Yellow
			The complex salt of axinic acid (Na, Al, Ca)	75470	Red
Chlorophyll a and b; The copper compound of chlorophyll and CHLOROPHYLLINE	75810	Green
			Aluminum	77000	In vain
Hydrated alumina	77002	In vain
			Hydrated aluminium silicate	77004	In vain
Ultramarine	77007	Blue
			Paratonere 101 and 102	77015	Red
Barium sulfate	77120	In vain
			The bismuth oxychloride and with micaceous mixture	77163	In vain
Calcium carbonate	77220	In vain
			Calcium sulfate	77231	In vain
Carbon black	77266	Black
			Pigment black 9	77267	Black
Medicinal charcoal, plant carbon	77268	Black
			Chromated oxide	77288	Green
The chromated oxide hydrate	77289	Green
			Alizarol saphirol 28, naphthol green 14	77346	Green
Pigment metal
	2	77400	Palm fibre

Gold	77480	Palm fibre
			Iron oxides and hydroxide	77489	Orange
Iron oxides	77491	Red
			The iron oxides hydrate	77492	Yellow
Iron oxides	77499	Black
			Six cyano group ferric acid ferrum (II) and six cyano group ferric acid ferrum (III) mixture	77510	Blue
Pigment white 18	77713	In vain
			Diphosphonic acid manganese ammonium salt	77742	Purple
Manganese phosphate, Mn ₃(PO ₄) ₂·7H ₂O	77745	Red
			Silver	77820	In vain
Titanium dioxide and composition thereof	77891	In vain
			Zinc oxide	77947	In vain
6,7-dimethyl-9-(1 '-D-ribityl) isoalloxazine, riboflavin	?	Yellow
			Caramel	?	Palm fibre
Capsorubin, capsorubin	?	Orange
			Betanin	?	Red
Benzopyralium salt, anthocyanidin	?	Red
			Bromocresol green	?	Green
Aluminium stearate, zinc stearate, magnesium stearate and calcium stearate	?	In vain
			Bromophenol blue	?	Blue
Azogeramine 95	?	Red

Food coloring is suitable as hair dyes equally very much.

Above-mentioned dyestuff can also be used as in conjunction with the effector molecule of peptide sequence (i) (ii), and is painted to (for example in tatooing) skin or fingernail.

If expectation, also can be easy in the keratin from skin, hair or fingernail to separate once more be connected in keratin-binding polypeptides sequence (i) effector molecule (ii).For this purpose, what might adopt is: for example, washing keratin, the effector molecule that will be connected in keratin-binding polypeptides (i) by this (ii) from its with keratic existing the combination cement out, and carry out saturated with the keratin in the cleaning mixture.Perhaps, also might be used to wash effector molecule off (ii) with high-load detergent (as SDS) washing.

Other preferred effector molecule (ii) is a fatty acid, the satisfied fatty acid that particularly has alkyl branches, preferred especially side chain arachidic acid, for example 18-methyl arachic acid.

Other preferred effector molecule (ii) is a carotenoid.According to the present invention, carotenoid is interpreted as being meant following one or as chemical compound and the esterification or the glycosylated derivative of mixture: beta-carotene, lycopene, phylloxanthin, astaxanthin, cryptoxanthin, kryptoxanthin, citroxanthin (citranaxanthin), canthaxanthin, annatto, β-apo--4-Radix Dauci Sativae aldehyde, β-apo--8-Radix Dauci Sativae aldehyde, β-apo--8-Radix Dauci Sativae ester, neurosporene, echinenone, adonirubin, violaxanthin, torulin (torulene), torularhodin.The preferred carotenoid that uses has beta-carotene, lycopene, phylloxanthin, astaxanthin, cryptoxanthin, citroxanthin and canthaxanthin.

Other preferred effector molecule (ii) is vitamin, particularly vitamin A and ester thereof.

For the object of the invention, retinoid refers to retinol (retinol) and derivant thereof, for example axerophthal (retinal), retinoic acid (tretinoin) and Davitin A (as acetic acid retinyl ester, Vitamin A propionate and retinyl palmitate).Thus, the term tretinoin comprises all trans retinoic acids and 13-cis-retinoic acid.Term retinol and retinal preferably include all trans-compounds.The retinoid that is preferred for suspension of the present invention is an alltrans retinol, hereinafter is referred to as retinol.

Other preferred effector molecule (ii) has from the vitamin of A, C, E and F family, provitamin and previtamin thing, and particularly 3, the two dehydroretinols of 4-, beta-carotene (provitamin of vitamin A); The cetylate of ascorbic acid (vitamin C) and ascorbic acid or glucosides or phosphate; Tocopherol, particularly alpha-tocopherol and ester thereof are as acetate, nicotinate, phosphate ester and succinate; The vitaminF, particularly linoleic acid, linolenic acid and the arachidonic acid that belong to essential fatty acid in addition in addition.

The vitamin of vitamin B group preferably used according to the invention, provitamin or previtamin or derivatives thereof, and 2-furanone derivatives comprise:

Vitamin B ₁, popular name thiamine, chemical name 3-[(4 '-amino-2 '-methyl-5 ' pyrimidine radicals) and methyl]-5-(2-ethoxy)-4-methylthiazol hydrochlorate;

Vitamin B ₂, popular name riboflavin, chemical name 7,8-dimethyl-10-(1-D-ribityl)-benzo [g] pteridine-2,4 (3H, 10H)-diketone.For example, riboflavin is present in the milk surum with free form, and can separate other from antibacterial and yeast and examine horizontal plain derivant.Being suitable for riboflavin stereoisomer of the present invention equally is lyxoflavin (lyxoflavin), and it can separate and have the D-arabinose alcohol radical that replaces the D-ribityl from fish flour or liver.

Vitamin B ₃, chemical compound nicotinic acid and nicotiamide (niacin amide) be so name often.According to the present invention, preferred nicotiamide.

Vitamin B ₅(pantothenic acid and pantothenylol).The preferred pantothenylol that adopts.Especially, adoptable pantothenylol derivant is a pantothenylol according to the present invention ester and ether, and the cationic derivative of pantothenylol.In another embodiment preferred of the present invention, except that pantothenic acid or pantothenylol, also might adopt the 2-furanone derivatives.Particularly preferred derivant is commercially available material, as dihydro-3-hydroxyl-4,4-dimethyl-2 (3H)-furanone, popular name pantolactone (pantolactone) (Merck), 4-methylol-gamma-butyrolacton (Merck), 3,3-dimethyl-2-hydroxyl-gamma-butyrolacton (Aldrich) and 2,5-dihydro-5-methoxyl group-2-furanone (Merck) is wherein particularly including all stereoisomers.

These chemical compounds can advantageously be given the performance of preserving moisture with skin lubrication to keratin-binding effector molecules of the present invention.

Vitamin B ₆Be not the same material, and be meant 5-methylol-2-picoline-3-01 derivatives, it is famous because of popular name pyridoxol, pyridoxamine and 2-methyl-3-hydroxy-4-formyl-5-hydroxymethylpyridine..

Vitamin B ₇(biotin) also is referred to as biotin or " skin vitamin ".Biotin be (3aS, 4S, 6aR)-2-oxo six hydrogen thiophene [3,4-d] imidazoles-4-valeric acid.

According to the present invention, extremely preferred pantothenylol, pantolactone, nicotiamide and biotin.

According to the present invention, might use suitable derivant (salt, ester, sugar, nucleotide, nucleoside, peptide and lipid).Preferred lipotropy oil-soluble inhibitor is tocopherol and derivant thereof in this family, gallium ester (gallic ester), flavonoid and carotenoid, and butylated hydroxy-methylbenzene/methoxybenzene.Preferred water soluble antioxidant is an aminoacid, as tyrosine and cysteine and their derivant, and the tannic acid tannic acid of plant origin particularly.

Triterpenoid compound, particularly triterpenic acid, for example ursolic acid, rosmarinic acid, belulinic acid Betulinic acid, boswellic acid and bryonolic acid.

Other preferred effector molecule is thioctic acid and suitable derivant (salt, ester, sugar, nucleotide, nucleoside, peptide and lipid) thereof.

Other preferred effector molecule (ii) is the ultraviolet filter agent, is meant the organic substance that can absorb ultraviolet and can radiate the energy that is absorbed with the form of long-wave radiation (as heat radiation) once more.Organic substance can be oil-soluble or water miscible.

The example of operable oil-soluble UV-B lightscreening agent has following material:

3-benzylidene camphor and derivant thereof are as 3-(4-methyl benzal) Camphora;

The 4-aminobenzoic acid derivative, preferred 4-(dimethylamino) benzoic acid 2-Octyl Nitrite, 4-(dimethylamino) benzoic acid 2-monooctyl ester and 4-(dimethylamino) amyl benzoate;

Cinnamate, preferred 4-methoxy cinnamic acid 2-Octyl Nitrite, 4-methoxy cinnamic acid propyl ester, 4-methoxy cinnamic acid isopentyl ester, 4-methoxy cinnamic acid isopentyl ester, 2-cyanogen-3-phenyl-cinnamic acid 2-Octyl Nitrite (1-Octyl acrylate);

Salicylate, preferred 2-Ethylhexyl salicylate, 4-isopropyl phenyl salicylate, 3,5,5-cyclonol salicylate (homomenthyl salicylate);

The derivant of benzophenone, preferred 2-hydroxyl-methoxy benzophenone, 2-hydroxyl-4-methoxyl group-4 '-methyldiphenyl ketone, 2,2 '-dihydroxy-4-methoxy benzophenone;

The benzylidene malonic acid ester, preferred 4-methoxyl group benzal malonic acid 2-Octyl Nitrite;

Pyrrolotriazine derivatives, for example 2,4,6-triphen amido-(to carbonyl-2 '-ethyl-1 '-hexyloxy)-1,3,5-triazines (octyl triazone) and dioctyl fourth ammonia triazinone (Uvasorb

HEB)

Propane-1,3-diketone, 1-(4-2-methyl-2-phenylpropane)-3-(4 '-methoxyphenyl) propane-1 for example, 3-diketone.

Suitable water-soluble substances has:

2-Phenylbenzimidazole-5-sulfonic acid and alkali metal salt, alkali salt, ammonium salt, alkylammonium salt, alkanol ammonium salt and glutamic acid ammonium salt.

The benzophenone sulfonic acid, preferred 2-hydroxyl-4-methoxy benzophenone-5-sulfonic acid and salt thereof;

3-benzylidene camphor sulfonic acid, for example 4-(2-oxo-3-borneol diene methyl) benzenesulfonic acid and 2-methyl-5-(2-oxo-3-borneol diene) sulfonic acid and salt thereof.

Especially preferably use cinnamate, preferred 4-methoxy cinnamic acid 2-Octyl Nitrite, 4-methoxy cinnamic acid isopentyl ester, 2-cyanogen-3-phenyl-cinnamic acid-2 Octyl Nitrite (1-Octyl acrylate);

Also preferably use benzophenone derivates, particularly 2-hydroxyl-4-methoxy benzophenone, 2-hydroxyl-4-methoxyl group-4 '-methyldiphenyl ketone, 2,2 '-dihydroxy-4-methoxy benzophenone; And use propane-1,3-diketone, 1-(4-2-methyl-2-phenylpropane)-3-(4 '-anisyl) propane-1 for example, 3-diketone.

Suitable typical ultraviolet light,long wave lightscreening agent has:

The benzoyl methane Derivatives, 1-(4 '-2-methyl-2-phenylpropane)-3-(4 '-methoxyphenyl) propane-1 for example, 3-diketone, the 4-tert-butyl group-4 '-methoxy dibenzoyl methane or 1-phenyl-3-(4 '-isopropyl phenyl) propane-1,3-diketone;

The hydroxy amino substitutive derivative of benzophenone, N for example, N-diethylamino (2-hydroxybenzoyl)-positive hexyl phenenyl formic acid esters.

Certainly also can adopt the mixture of ultraviolet light,long wave and UV-B lightscreening agent.

Following table provides other suitable ultraviolet filtering agent material.

Numbering	Material	CAS numbers (=acid)
			1	The 4-amino benzoic Acid	150-13-0
2	3-(4 '-trimethylamine) benzal thatch alkane-2-ketone methylsulfuric acid ester	52793-97-2
			3	3,3,5-trimethylcyclohexyl salicylate (homosaligenin)	118-56-9
4	2-hydroxyl-4-methoxyl group-benzophenone (oxybenzone)	131-57-7
			5	2-Phenylbenzimidazole-5-sulfonic acid and potassium salt, sodium salt and triethanolamine salt	27503-81-7
6	3,3 '-(1, the 4-phenylenedimethylidyne) two (7,7-dimethyl-2-oxo dicyclo [2.2.1] heptane-1-methane-sulfonic acid) and salt thereof	90457-82-2
			7	Poly-ethoxyethyl 4-two (poly-ethoxy) Aminobenzoate	113010-52-9
8	2-ethylhexyl 4-dimethylaminobenzoic acid ester	21245-02-3
			9	The 2-Ethylhexyl salicylate	118-60-5
10	2-isopentyl 4-Methoxycinnamate	71617-10-2

11	2-ethylhexyl 4-Methoxycinnamate	5466-77-3
			12	2-hydroxyl-4-methoxyl group benzophenone-5-sulfonic acid (sulisobenzone) and sodium salt thereof	4065-45-6
13	3-(4 '-sulfo-benzal) thatch alkane-2-ketone and salt thereof	58030-58-6
			14	3-benzal thatch alkane-2-ketone	16087-24-8
15	1-(4 '-isopropyl phenyl)-3-phenyl-propane-1, the 3-diketone	63260-25-9
			16	4-isopropyl phenyl salicylate	94134-93-7
17	3-imidazol-4 yl acrylic acid and ethyl ester thereof	104-98-3
			18	2-cyano-3,3-diphenyl ethyl acrylate	5232-99-5
19	2 '-ethylhexyl 2-cyano group-3,3-diphenylacrylate ester	6197-30-4
			20	Methyl 2-aminobenzoate or 5-methyl-2-(1-Methylethyl)-2-Aminobenzoate	134-09-8
21	Para-amino benzoic acid glyceride or 1-glyceryl 4-Aminobenzoate	136-44-7
			22	2,2 '-dihydroxy-4-methoxyl group benzophenone (dihydroxyphenyl ketone)	131-53-3
23	2-hydroxyl-4-methoxyl group-4-methyldiphenyl ketone (mexenone)	1641-17-4
			24	The triethanolamine Salicylate	2174-16-5
25	Dimethoxyphenyl glyoxalic acid or 3,4-Dimethoxyphenyl glyoxalic acid sodium	4732-70-1
			26	3-(4 '-sulfo-) benzal thatch alkane-2-ketone and salt thereof	56039-58-8
27	The 4-tert-butyl group-4 '-methoxy dibenzoyl methane	70356-09-1
			28	2,2 ', 4,4 '-tetrahydroxybenzophenone	131-55-5
29	2,2 '-di-2-ethylhexylphosphine oxide [6-(2H-benzotriazole-2-yl)-4-(1,1,3, the 3-tetramethyl butyl) phenol]	103597-45-1
			30	2,2 '-(1, the 4-phenylene) is two-1H-benzimidazole-4,6-disulfonic acid, sodium salt	180898-37-7
31	2, two [4-(2-ethyl hexyl oxy)-2-hydroxyl] phenyl-6-(4-methoxyl group-phenyl) of 4--(1,3,5) triazine	187393-00-6
			32	3-(4 '-methyl benzal) Camphora	36861-47-9

33	Poly-ethoxyethyl-4-two (poly-ethoxy) p-aminobenzoate	113010-52-9
			34	2,4 dihydroxy benzophenone	131-56-6
35	2,2 '-dihydroxy-4,4 '-dimethoxy-benzophenone-5,5 '-sodium disulfonate	3121-60-6
			36	2-[4-(diethylamino)-2-hydroxy benzoyl]-benzoic acid hexyl ester	302776-68-7
37	2-(2H-2-benzotriazole-2-yl)-4-methyl-6-[2-methyl-3-[1,3,3,3-tetramethyl-1-[(trimethyl silyl) the oxygen base]-the disiloxane base] propyl group]-phenol	155633-54-8

Except that the main photoprotection material of two above-mentioned classes, also might adopt the secondary opacifier of antioxidation dosage form, described opacifier can interrupt when ultraviolet runs through skin and inductive chain type photochemical reaction.Its representative instance has superoxide dismutase, catalase, tocopherol (vitamin E) and ascorbic acid (vitamin C).

Other class has the skin of pair ultraviolet damage to have the counter-stimulus of antiinflammatory action.The example of this class material is Bisabolol terpene alcohol, phytol and phytantriol.

Effector molecule (ii) with being connected of keratin-binding polypeptides sequence (i)

Effector molecule (ii) is connected on the peptide sequence (i) with keratin binding affinity.(i) and the connection (ii) both can be covalent bond, also can be based on the interaction of ion or Van der Waals force.

The preferably covalently key.For example, this can be undertaken by the side chain of peptide sequence (i), particularly by amido functional group or hydroxy functional group or carboxylate functional group or thiol functionalities.Preferably the amido functional group by one or more lysine residues, the mercaptan by one or more cysteine residues or N-terminal or C-terminal functional group by polypeptide (i) connect.The amino acid functional group in being present in peptide sequence (i), also the aminoacid (as cysteine, lysine, aspartic acid, glutamic acid) with appropriate functional group might be connected in peptide sequence (i), or the aminoacid of peptide sequence (i) is replaced by such amino acid functional group.

Can directly carry out effector molecule (ii) with being connected of peptide sequence (i), promptly as be present in already (i) and (ii) among two kinds of chemical functional groups covalently bound, for example the amido functional group of (i) is connected in (ii) carboxylate functional group with the generation amide.But, also can connect by so-called joint (promptly being at least dual functional molecule), described joint is connected with (i) by a kind of functional group, and is connected in (ii) by one or more other functional groups.

If effector molecule (ii) is made up of peptide sequence equally, then (i) with (ii) can be connected the i.e. continuous peptide sequence of forming by two parts (i) and sequence (ii) by the mode of so-called fusion rotein.

Also so-called spacer element might be inserted in (i) and (ii) between, the peptide sequence that for example has the potential cracking site of protease, lipase, esterase, phosphatase, hydrolytic enzyme, perhaps make the oligopeptide sequence or the peptide sequence of the easy purification of fusion rotein, for example so-called histidine-tagged, i.e. oligo-histidine residue.

Spacer element can be made up of alkyl chain, ethylene glycol and Polyethylene Glycol in addition.

Particularly preferably, joint and/or spacer element have the potential cracking site of protease, lipase, esterase, phosphatase, hydrolytic enzyme, promptly are the enzyme cleavables.

For example, disclose the example of the joint of the enzyme cleavable that can be used for molecule of the present invention among the WO98/01406, clearly its full content has been quoted as a reference at this.

Particularly preferred joint and sept are hot cleavable, light cleavable.

Corresponding chemical constitution is well known to a person skilled in the art, and can be incorporated into molecular moiety (i) and (ii) between.

Under nonprotein effector molecule and situation that peptide sequence (i) is connected, preferably by polypeptide (i) but the residue (side-chain radical, C or N-terminal) of going up functional groupization carry out, it is covalently attached to the chemical functional group of effector molecule.

In this case, preferably amino, mercaptan or the hydroxy functional group by polypeptide (i) connects, and for example, under suitable situation, after they are activated, can carry out corresponding amide, thioesters or ester with effector molecule carboxyl functional group (ii) and be connected.

Other preferred peptide sequence (i) and the effector molecule use that is connected customization (tailored) joint that is (ii).Such joint has two or more so-called anchoring groups, and it can (ii) couple together peptide sequence (i) and one or more effector molecules.For example, the anchoring group that is used for peptide sequence (i) can be a thiol functionalities, and by means of this mercaptan, joint carries out disulfide bond with the cysteine residues of polypeptide (i) and is connected.For example, being used for effector molecule anchoring group (ii) can be carboxyl functional group, and by means of this carboxyl, joint can carry out ester bond with effector molecule hydroxy functional group (ii) and be connected.

Use such customization joint, make connection can mate the effector molecule of expectation exactly.Thus, also the mode of a large amount of effector molecules with regulation might be connected on the peptide sequence (i) in addition.

Used joint depends on treats link coupled functional group.For example, suitable example has by following sulfydryl reactive group and polypeptide (i) coupling: maleimide, pyridyl disulfide, alpha-halogen acetyl, vinyl sulfone(Remzaol, sulfato alkyl sulfone (the preferably sulfuric acid root closes ethyl sulfone), and by following groups and the (ii) link coupled molecule of effector molecule:

-sulfydryl reactive group is (as maleimide, pyridyl disulfide, a-halo acetyl, vinyl sulfone(Remzaol, sulfato alkyl sulfone (the preferably sulfuric acid root closes ethyl sulfone);

-amine reactive group (as succinimide ester, carbodiimide, methylol phosphine, imino-ester, PFP ester or the like);

-sugar or oxosugar reactive group (as hydrazides or the like);

-carboxyl reactive group (as carbodiimide or the like);

-hydroxyl reaction base (as isocyanates or the like);

-thymus pyrimidine reactive group (as psoralen or the like);

-non-selectivity group (as aromatic yl azide or the like);

-light reaction group (as full-fluorinated benzene azide or the like);

-metal complex group (as EDTA, six histidine (hexahis), ferritin);

-antibody and fragment thereof (as F (ab) fragment, the catalytic antibody of single-chain antibody, antibody).

Alternative possibilities is directly to put together between active substance/effector molecule and keratin binding structural domain, as being the known cross-linking agent of technical staff by carbodiimide, glutaraldehyde, above-mentioned cross-linking agent or other.

If expectation also can be easy to separate once more keratin-binding effector molecules of the present invention from keratin.For this purpose, what might adopt is: for example, washing keratin, by this with keratin-binding effector molecules from its with keratic existing the combination cement out, and carry out saturated with the keratin in the cleaning mixture.Therefore, might carry out a large amount of effector molecules to keratic reversible adhering to.Perhaps, also might be used to wash off effector molecule with high-load detergent (as SDS) washing.

Keratin-binding effector molecules according to the present invention particularly is with a wide range of applications in skin and hair-care, animal care, leather nursing and the leather processing at people's cosmetics.

Keratin-binding effector molecules of the present invention is preferred for skin, fingernail and hair cosmetic composition.They allow the high concentration and the long-acting time of skin, fingernail and hair nursing or skin, fingernail and hair protective effect thing.

Be used to produce that hair is made up or the suitable adjuvant of skin cosmetic product is well known to those skilled in the art, and can be referring to the cosmetic handbook, Schrader for example, Grundlagen und Rezepturen, derKosmetika, H ü thig Verlag, Heidelberg, 1989, ISBN 3-7785-1491-1.

In another embodiment, described hair is made up or the skin cosmetic product is used for nursing or protection skin or hair, and can be following form: Emulsion; dispersion, suspension, aqueous tenside goods; emulsion, lotion, cream; face cream, unguentum, gel; granule, face powder, the stick product is lipstick for example; foamed glue, aerosol or spray.Such dosage form is very suitable for local goods.Suitable Emulsion is oil-in-water emulsion and water-in-oil emulsion or microemulsion.

Hair cosmetic or skin cosmetic product are applied to skin (part) or hair usually.Thus, local goods refer to be suitable for the active substance FINE DISTRIBUTION to skin and be preferably the goods that can see through the skin absorbs form.The example that is suitable for this purpose has aqueous and alcohol-water solution, spray, foamed glue, foam aerosol, unguentum, aqueous gel, oil-in-water or water-in-oil emulsion, microemulsion or cosmetic stick product.

In the preferred embodiment of make-up composition of the present invention, compositions comprises carrier.Preferred carrier is water, gas, the liquid based on water, oil, gel, Emulsion or microemulsion, dispersion or their mixture.Described carrier is that skin is well tolerable.Good especially local goods are hydrogel adhesive, Emulsion or microemulsion.

Spendable emulsifying agent is nonionic surfactant, zwitterionic surfactant, amphoteric surfactant or anion emulsifier.Emulsifier based in compositions be 0.1% to 10% by weight, preferred 1% to 5% amount is present among the compositions of the present invention.

For example, can use and be selected from following at least a surfactant and be used as nonionic surfactant:

2 to 30mol oxirane and/or 0 to 5mol expoxy propane and straight-chain fatty alcohol with 8 to 22 C atoms, with the adduct that has the fatty acid of 12 to 22 C atoms and in alkyl, have the alkyl phenol of 8 to 15 C atoms;

The C of the adduct of 1 to 30mol oxirane and glycerol _12/18Fatty-acid monoester and diester;

Have the saturated and unsaturated fatty acid of 6 to 22 carbon atoms and glycerol monoesters and diester and the sorbitan monoester and the diester of ethylene oxide adduct thereof;

The alkyl monoglycosides and oligosaccharide glycosides and the ethoxylation analog thereof that in alkyl, have 8 to 22 carbon atoms;

The adduct of 15 to 60mol oxirane and Oleum Ricini and/or hardened castor oil;

Polyol ester, particularly polyglycerin ester, for example poly-ricinoleate of polyglycerol, the poly-12-hydroxy stearic acid ester of polyglycerol or polyglycerol dimer.Compound mixture from a large amount of these class materials equally also is suitable;

The adduct of 2 to 15mol oxirane and Oleum Ricini and/or hardened castor oil;

With the unsaturated or saturated C of straight or branched _6/22Fatty acid, ricinoleic acid and 12-hydroxy stearic acid and glycerol, polyglycerol, tetramethylolmethane, dipentaerythritol, sugar alcohol (as Sorbitol), alkyl polyglucoside (as methylglycoside, butyl glycoside, dodecyl glucosides) and polyglycosides (as cellulose) are the partial ester on basis.

Mono alkyl phosphate, phosphate dialkyl ester and trialkylphosphate and PEG-mono alkyl phosphate, PEG-phosphate dialkyl ester and/or PEG-trialkylphosphate and their salt;

Wool wax alcohol;

Polysiloxanes-poly-alkane copolyether and corresponding derivant;

As the mixed ester of disclosed tetramethylolmethane, fatty acid, citric acid and aliphatic alcohol among the DE1165574, and/or have fatty acid, methyl glucoside and the polyhydric alcohol of 6 to 22 carbon atoms, the mixed ester of preferred glycerol or polyglycerol, and

Polyglycols;

Betanin.

Also can use zwitterionic surfactant as emulsifying agent.The surfactant that is referred to as zwitterionic surfactant is the surfactant that has at least one quaternary ammonium group and at least one carboxylate or a sulfonate group in molecule.Specially suitable zwitterionic surfactant is so-called betanin; as N-alkyl-N; N-dimethylglycine ammonium; cocos nucifera oil alkyl dimethyl glycine ammonium for example; N-amidopropyl-N; N-dimethylglycine ammonium for example has the cocamidopropyl propyl-dimethyl glycine ammonium and the 2-alkyl-3-carboxymethyl-3-hydroxyethyl imidazole quinoline of 8 to 18 carbon atoms and cocamidopropyl ethyl-hydroxyethyl carboxymethyl glycinate respectively in alkyl or acyl group.Particularly preferred fat amide derivant is well-known derivant with CTFA name cocamidopropyl betaine.

Same suitable emulsifying agent is an amphoteric surfactant.Amphoteric surfactant refers to except C _8,18Beyond alkyl or the acyl group, in molecule, comprise at least one free amine group and at least one COOH or SO ₃The H group also can form the surface active cpd of inner salt.Suitable examples of amphoteric surfactants is N-alkyl glycinate, N-alkyl propionate, N-alkyl amino butyrate, N-alkyl imido malonate, N-ethoxy-N-alkyl amino propyl group glycinate, N-alkyltaurate, N-alkyl sarcosine salt, 2-alkyl aminopropionic acid salt and the alkyl glycinate that has about 8 to 18 carbon atoms in alkyl respectively.

Particularly preferred amphoteric surfactant is N-coco alkyl amino propionic acid salt, coconut oleoyl amine ethylamino propionate and C12/18-acyl sarcosinates.Except that amphoteric emulsifier, same suitable emulsifying agent also has the quaternary emulsifying agent, the emulsifying agent of preferred especially four esters types, preferred tetramethylated difatty acid triethanolamine ester salts.Also spendable anion emulsifier is alkyl ether sulphate, sulphuric acid monoglyceride, fatty acid sulfate, sulfosuccinate and/or ether carboxylic acid.

Suitable oily matter is based on the Guerbet alcohol of the aliphatic alcohol with individual, preferred 8-10 the carbon atom of 6-18, straight chain C ₆-C ₂₂Fatty acid and straight chain C ₆-C ₂₂The ester of aliphatic alcohol, side chain C ₆-C ₁₃Carboxylic acid and straight chain C ₆-C ₂₂The ester of aliphatic alcohol, straight chain C ₆-C ₂₂The ester of fatty acid and branched-chain alcoho (particularly 2-Ethylhexyl Alcohol), the ester and/or the guerbet alcohol of straight chain and/or branched chain fatty acid and polyhydroxy-alcohol (for example propylene glycol, dimer diol or trimer triol) are based on C ₆-C ₁₀The triglyceride of fatty acid is based on C ₆-C ₁₈Liquid glycerin monoesters/the diester of fatty acid, three ester admixtures, C ₆-C ₂₂Aliphatic alcohol and/or guerbet alcohol and aromatic carboxylic acid's's (particularly benzoic acid) ester, C ₂-C ₁₂The ester of dicarboxylic acids and straight or branched alcohol with 1 to 22 carbon atom or with the ester of polyhydric alcohol, vegetable oil, branched-chain primary alcohol, the cyclohexane extraction of replacement, straight chain C with 2 to 10 carbon atoms and 2 to 6 hydroxyls ₆-C ₂₂The aliphatic alcohol carbonic ester, guerbet carbonic ester, benzoic acid and straight chain and/or side chain C ₆-C ₂₂Alcohol is (as FinsoIv TN) ester, dialkyl ethers, the open-loop products of epoxidized fatty acid ester and polyhydric alcohol, silicone oil and/or aliphatic series or cycloalkane.Adoptable other oily matter is a silicone compounds, for example dimethicone, methyl phenyl silicone, ring silicone, and the silicone compounds of amino, fatty acid, alcohol, polyethers, epoxy radicals, fluorine, alkyl and/or glucosides modification, the silicone compounds of described modification at room temperature both can be that liquid state also can be a resin form.Oily matter based on compositions be by weight 1% to 90%, preferred 5% to 80% and preferred especially 10% to 50% amount be present among the compositions of the present invention.

By suitable compound being coupled to the continuous action that can prolong significantly on the keratin-binding polypeptides (i) to skin.Carry out coupling as mentioned above, and prepare and use by method known to those skilled in the art.The effector molecule that is particularly suitable for doing deodorizer (ii) is aromatic oil, cyclodextrin, ion-exchanger, zinc ricinoleate, antibiotic/bacteriostatic compound (as DCMX, Irgasan DP 300, TCC).

What be applicable to antiperspirant is: tannic acid and zinc/aluminum salt.

The Another Application field of material of the present invention is the treatment or the preventive use of some skin and mucous membrane disorder.The active substance that is used for the treatment of/prevents preferably is combined in more by force and for more time particularly in oral cavity, pharyngeal and nasal septum film by the keratin binding structural domain.Its concrete range of application is:

-viral disease (as herpes, Coxsackie virus, varicella zoster, cytomegalovirus etc.);

-bacterial disease (as tuberculosis, syphilis etc.);

-fungal disease (as Candida disease, Cryptococcus disease, histoplasmosis, aspergillosis, mucormycosis etc.);

-neoplastic disease (as melanoma, adenoma etc.);

-autoimmune disease (as chronic pemphigus, large blister quasi-Pemphigus, systemic lupus erythematosus etc.);

-sunburn;

-parasitic infection (as tick, demodicid mite, flea etc.);

-insecticide contact (as the blood sucking insect, for example malarial mosquito etc.).

Can will be suitable for treating or the material (as corticoid, immunosuppressant compounds, antibiotic, anti-mildew material, antiviral compound, insect repellent or the like) that prevents is coupled on the keratin-binding polypeptides (i) by means of above-mentioned joint (according to the joint for the treatment of that link coupled functional group is optimized).

Embodiment 1: expression vector and production strain

Multiple expression vector is carried out the test of express keratin binding structural domain (KBD).For this purpose, can use multiple promoter (as IPTG induction type, rhamnose induction type, arabinose induction type, methanol evoked, constitutive promoter, or the like).Same tested K BD as fusion rotein (as with the fusion [B.subtilis, SWISS-PROT:P37527, PDX1] of thioredoxin or eGFP or YaaD) construct of expressing.Use multiple expression system to express the combination of described KBD-B (keratin binding structural domain B) and KBD-C (keratin binding structural domain C) and these two kinds of domain KBD-BC herein.The vector construction body of mentioning is for claims and nonrestrictive.

Explanation is the carrier collection of illustrative plates of IPTG induction type carrier pQE30-KBD-B (Fig. 3), methanol evoked carrier pLib15 (Fig. 4) and pLib16 (Fig. 5) and induction type carrier pLib19 (Fig. 6) as an example.The method that is used for KBD-C also can be similar to the structure and the expression of described carrier.

For example, for expressing K BD, use multiple production host, for example escherichia coli (E.coli) bacterial strain is (referring to embodiment 2; As XL10-Gold[Stratagene], BL21-CodonPlus[Stratagene], or the like).But, also can use other antibacterial to produce host, for example bacillus megaterium (Bacillus megaterium) or bacillus subtilis (Bacillus subtilis).With regard to the expression in the bacillus megaterium, method is similar to Barg, H., Malten, M. and Jahn, D. (2005) .Proteinand vitamin production in Bacillus megaterium.In Methods inBiotechnology-Microbial Products and Biotransformations (Barredo, J.-L. edits).

The fungus production strain of using is that pichia pastoris phaff (Pichia pastoris) is (referring to embodiment 3; As GS115 and KM71[all from Invitrogen]; Or the like) and aspergillus nidulans (Aspergillusnidulans) (referring to embodiment 4; As RMS011[Stringer, MA, Dean, RA, Sewall, TC, Timberlake, WE (1991), Rodletless, a new Aspergillus developmentalmutant induced by direct gene activation.Genes Dev 5:1161-1171] and SRF200[Karos, M, Fischer, R (1999), Molecular characterization ofHymA, an evolutionarily highly conserved and highly expressed protein ofAspergillus nidulans.Mol Genet Genomics 260:510-521], or the like).But, also might use other fungus to produce the host, for example be used for the aspergillus niger (Aspergillus niger) (KBD expresses and is similar to EP0635574A1 and/or WO98/46772) that KBD expresses.

Embodiment 2: by IPTG inducible promoter such as expression plasmid pQE30-KBD-B at expression in escherichia coli KBD

In order to express, use multiple production host, for example multiple e. coli strains (as XL10-Gold[Stratagene], BL21-CodonPlus [Stratagene], or the like), bacillus megaterium, bacillus subtilis or the like.

Explanation as an example, what describe in the literary composition is by cloning and expressing K BD-B with the pQE30-KBD-B transformed into escherichia coli:

The clone of pQE30-KBD-B

-prepare λ-MaxiDNA (λ DNA Maxi test kit, Qiagen) (BD Bioscience, Clontech, human keratinocyte cDNA, foreskin, the primary culture of logarithmic (log) phase, carrier: λ gt11) from human keratinocyte cDNA library.

PCR uses following oligonucleotide to carry out:

Bag 43 (5 '-GGTCAGTTACGTGCAGCTGAAGG-3 ') and

Bag?44(5′GCTGAGGCTGCCGGATCG-3’)。

-from agarose gel, downcut the PCR product of about 1102 bp of resulting size, and carry out purification.

The PCR product of-use purification carries out the PCR second time then as template:

Use following oligonucleotide:

Bag 53:(5 '-CGCGCCTCGAGCCACATACTGGTCTGC-3 ') and

Bag?51(5′-GCTTAGCTGAGGCTGCCGGATCG-3’)。

-from the PCR product of the about 1073bp of the resulting size of agarose gel cutting-out, purification also is cloned in the following carrier: pCR2.1-TOPO (Invitrogen).

-then with carrier pCR2.1-TOPO+KBD-B (5027bp) transformed into escherichia coli that obtains and increase, use xhoI and EcoRI enzyme action then, and with the KBD-B fragment cloning that obtains to pBAD/HisA (Invitrogen; Same with xhoI and EcoRI enzyme action) in.

-with SacI and the StuI new carrier pBAD/HisA+KBD-B (5171bp) that forms of enzyme action once more, and with the KBD-B fragment cloning that obtains to pQE30 (Qiagen; With SacI and SmaI enzyme action) in.With the expression vector pQE30-KBD-B (4321bp that obtains; See Fig. 3) be used for following KBD-B and express.

By the KBD-B of carrier pQE30-KBD-B, in peptide sequence SEQ ID NO:1, the 2193-2481 position, comprise the aminoacid MRGSHHHHHHGSACEL of N-terminal and the aminoacid GVDLQPSLIS of C-terminal in addition at expression in escherichia coli.

By pQE30-KBD-B at expression in escherichia coli KBD-B

-inoculate pre-culture from the plate or the glycerol culture of the e. coli strains that transformed by pQE30-KBD-B (as XL10-Gold[Stratagene]).According to the size of master culture, in test tube or little flask, inoculate with LB culture medium (about 1: 100).

-use antibiotic (for pQE30-KBD-B, ampicillin is 100 μ g/ml) according to used bacterial strain.

-carry out incubation 250rpm and 37 ℃.

-with pre-culture with about 1: 100 the inoculation master culture (master culture: contain corresponding antibiotic LB culture medium or suitable minimal medium).Carry out incubation in 250rpm and 37 ℃.

-surpass at 0.5 o'clock in OD (600nm) value, induce with 1mM IPTG.

-induce 4h after, centrifuge cell.

Method is similarly in fermentation tank, though might when higher OD value, induce, thus cell and protein output improved in a large number.

Embodiment 3: use methanol inducible promoters, as expression plasmid pLib 15 and pLib 16, carry out cell inner expression and the secreting, expressing (shaking bottle) of KBD by the pichia pastoris phaff bacterial strain

For the expression of KBD, use multiple pichia pastoris phaff strain, for example GS115 and KM71 (Pichia Expression test kit, Version M; Invitrogen LifeTechnologies).

Explanation as an example, what describe in the literary composition is to come expressing K BD-B with the pichia pastoris phaff that pLib15 (cell inner expression, carrier is seen Fig. 4) or pLib16 (secreting, expressing, carrier is seen Fig. 5) transform.

-in order to make up pLib15, (embodiment 2 with carrier pQE30-KBD-B, Fig. 3) as template, use following oligonucleotide by PCR the increase dna fragmentation of encoded K BD-B of the about 930bp of size: Lib148 (5 '-GCTAAGGAATTCACCATGCATCACCATCACCATCACGAGCCA CATACTGGTCTGCT-3 ') and Lib149 (5 '-GCTGGAGAATTCTCAGCTAATTAAGCTTGGCTGCA-3 ').All introduced the EcoRI restriction enzyme site at the two ends of PCR product herein.

-in order to make up pLib16, (embodiment 2 with carrier pQE30-KBD-B, Fig. 3) as template, use following oligonucleotide by PCR the increase dna fragmentation of encoded K BD-B of the about 930bp of size: Lib149 (5 '-GCTGGAGAATTCTCAGCTAATTAAGCTTGGCTGCA-3 ') and Lib150 (5 '-GCTAAGGAATTCCATCACCATCACCATCACGAGCCACATACTGGTCTGCT-3 ').All introduced the EcoRI restriction enzyme site at the two ends of PCR product herein.

-digest by EcoRI with oligonucleotide Lib148/Lib149 amplification PCR products, and be connected to the EcoRI enzyme action carrier pPIC3.5 (Pichia Expression test kit, Version M, Invitrogen) in.Check order by the carrier pLib15 (Fig. 4) that connection is obtained, verify correct KBD-B amplification.

-digest by EcoRI with oligonucleotide Lib149/Lib150 amplification PCR products, and be connected to the EcoRI enzyme action carrier pPIC9 (Pichia Expression test kit, Version M, Invitrogen) in.Check order by the carrier plib16 (Fig. 5) that connection is obtained, verify correct KBD-B amplification.

-use ring-type and the linearizing carrier pLib15 of StuI and pLib16 to transform the electroporation competent cell and the spheroplast of pichia pastoris phaff.

-carry out Southern engram analysis transformant by PCR and use chromosomal DNA.

-for pre-culture, the pichia pastoris phaff transformant of inoculation expressing K BD-B from flat board or glycerol culture.According to the size of master culture, (the Pichia-Expression-test kit, Version M Invitrogen) inoculates to use MGY, BMG or BMGY culture medium (about 1: 100) in test tube or little flask.

-with culture in 250-300rpm and 30 ℃ of incubations until OD ₆₀₀=2-6.

-at room temperature came collecting cell in centrifugal 5 minutes by 1500-3000 * g.

-for master culture, with the cell precipitation collected with OD ₆₀₀=1 be dispersed in the mM, the BMM that contain methanol or BMMY culture medium (the Pichia-Expression-test kit, Version M, Invitrogen) in so that abduction delivering.

-with master culture in 250-300rpm and 30 ℃ of incubation 1-96h.

-add 100% methanol by every 24h to keep until 0.5% methanol final concentration and induce.

-under the situation of cell inner expression, after main cultivation finishes, collect and smudge cells by the Menton-Gaulin method.

-under the situation of secreting, expressing, collect the culture supernatant, and direct purification KBD-B therefrom.

-in peptide sequence SEQ ID NO:1 the 2193-2481 position, the KBD-B of cell inner expression comprises the aminoacid MHHHHHH of N-terminal and the aminoacid GVDLQPSLIS of C-terminal in addition in pichia pastoris phaff (pLib15).

-in peptide sequence SEQ ID NO:1 the 2193-2481 position, the KBD-B of secreting, expressing comprises the aminoacid MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFS NSTNNGLLFINTTIASIAAKEEGVSLEKREAEAYVEFHHHHHH of N-terminal and the aminoacid GVDLQPSLIS of C-terminal in addition in pichia pastoris phaff (pLib16).

In peptide sequence SEQ ID NO:1, the 2193-2481 position, comprise the aminoacid YVEFHHHHHH of N-terminal and the aminoacid GVDLQPSLIS of C-terminal in addition by pichia pastoris phaff (pLib16) processing and excretory KBD-B.

Embodiment 4: use induction type alcA promoter, as expression plasmid pLib 19, by aspergillus nidulans bacterial strain expressing K BD (shaking bottle)

In order to express, use the aspergillus nidulans wild-type strain, for example RMS011 or SRF200.Explanation as an example, what describe herein is to come expressing K BD-B with the aspergillus nidulans that pLib19 (Fig. 6) transforms.

-in order to make up pLib19, (embodiment 2 with carrier pQE30-KBD-B, Fig. 3) as template, use following oligonucleotide by PCR the increase dna fragmentation of encoded K BD-B of the about 900bp of size: Lib151 (5 '-CACCATGCATCACCATCACCATCACGAGCCACATACTGGTCTGCT-3 ') and Lib152 (5 '-GCTAATTAAGCTTGGCTGCA-3 ').The PCR product is linked carrier pENTR/D (pENTR ^TMDirectional TOPO

The Cloning test kit, Version E, Invitrogen) in.Verify correct KBD-B amplified matter by checking order.

-use " Gateway

LR clonaseTM enzyme mix " (Invitrogen), the dna fragmentation of encoded K BD-B is recombinated among the carrier pMT-OvE (Toews MW, Warmbold J, Konzack S; Rischitor P, Veith D, Vienken K; Vinuesa C, Wei H, Fischer R; Establishment of mRFP1 as a fluorescent marker in Aspergillus nidulansand construction of expression vectors for high-throughput protein taggingusing recombination in vitro (GATEWAY). (2004) Curr Genet 45:383-389).This can prepare carrier pLib19 (Fig. 6).

-use circular vectors pLib19 to transform the protoplast of aspergillus nidulans wild-type strain.Carry out Southern engram analysis transformant by PCR and use chromosomal DNA.

-be the pre-cultivation of carrying out the aspergillus nidulans transformant of expressing K BD-B, in the 500ml flask, with 10 ⁶-10 ⁷Spore inoculating 100ml minimal medium (0.6%NaNO ₃, 0.152%KH ₂PO ₄, 0.052%KCl[pH6.5], 0.8% glucose, 0.05%MgSO ₄, 1ml trace element solution [1g/lFeSO ₄* 7H ₂O, 8.8g/l ZnSO ₄* 7H ₂O, 0.4g/l CuSO ₄* 5H ₂O, 0.15g/l MnSO ₄* 4H ₂O, 0.1g/l Na ₂B ₄O ₇* 10H ₂O, 0.05g/l (NH ₄) ₆Mo ₇O ₂₄* 4H ₂O]+the specific fill-in of bacterial strain) or 100ml complete medium (2% malt extract, 0.1% peptone, the specific fill-in of 2% glucose+bacterial strain), and in 200-250rpm and 37 ℃ of incubation 16-24h.

-after pre-the cultivation, collect fungal mycelium, with distilled water wash and change in the flask that contains the fresh minimal medium of 100-500ml by filtering.In this main medium, use 0.1% fructose to replace glucose to be used as carbon source.In order to induce KBD to express, in culture medium, add ethanol (1% final concentration) or glycerol (50mM) or sodium acetate (50mM) or ethamine or threonine in addition.

Additive of mentioning that is used for abduction delivering and unrestricted claim.With master culture in 200-250rpm and 37 ℃ of other incubation 5-48h.

-after cultivating end, collected fungal mycelium in centrifugal 5 minutes by 1500-3000 * g under the room temperature, and carry out fragmentation by Menton-Gaulin.

-in peptide sequence SEQ ID NO:1 the 2193-2481 position, the KBD-B that expresses in aspergillus nidulans (pLib19) comprises the aminoacid MHHHHHH of N-terminal and the aminoacid KGGRADPAFLYKVVMIRLLTKPERKLLEGGPGTQLLFPLVRVNCALGVIMVIAVSC VKLLSAHNSTQHTSRKHKV of C-terminal in addition.

Embodiment 5: cell breakage and inclusion body purification (pQE30-KBD-B)

Can directly use institute behind the KBD purification of solubility expression.The following purification of KBD (as in inclusion body) that non-solubility is expressed:

-centrifugal fermented material is suspended in precipitation in the 20mM phosphate buffer (pH=7.4), and carries out fragmentation by Menton-Gaulin.

The cell of-recentrifuge fragmentation (15000g) is handled the therefrom precipitation of gained with 20mm phosphate, 500mm NaCl and 8M carbamide, and is stirred.

(dissolving of inclusion body)

-adjusting supernatant pH to 7.5.

-and then carry out centrifugally, and supernatant is applied on the agarose gel post of Ni chelating.

Embodiment 6: the purification of keratin binding structural domain B on the agarose gel post of Ni chelating

By on the Ni post to the absorption of His label, but chromatogram purification KBD.

Column material: high performance Ni-agarose gel,

The Amersham Biosciences number of ordering No.:17-5268-02

Material is inserted in the post (as diameter 2.6cm, height 10cm), and carried out balance with buffer A+4% buffer B (being equivalent to the 20mM imidazoles).

Use Superloop ( The KTA system) (the about 5ml/min of flow velocity) is added to protein extract (referring to cell breakage and inclusion body purification) on the post when pH 7.5.

Behind the strutting, wash with buffer A+20mM imidazoles.Carry out eluting with buffer B (buffer A of 500mM imidazoles).

Use fraction collector, collect the eluent in the fraction.

Buffer A: 20mM sodium dihydrogen phosphate

500mM?NaCl

8M carbamide

pH＝7.40

Buffer B: 20mM sodium dihydrogen phosphate

500mM?NaCl

8M carbamide

The 500mM imidazoles

pH＝7.40

Embodiment 7: the renaturation of keratin binding structural domain B.

As follows, renaturation also activates the keratin binding structural domain (as from inclusion body) that non-solubility is expressed:

Method 1: intermittently dialysis

8 M carbamide (Ni chelating eluent, HiTrap) in, 6.5ml Cellytic IB (Sigma, the number of ordering No.C5236) and 5mM DTT are joined in the 6.5ml KBD-B inclusion body.To treat that then the solution of renaturation pours in the Dialysis tubing (Spectrum:Spectra Por MWCO:12-14kD).

At 4 ℃,, dialysed about 12 hours with the 6M urea liquid of 1L by careful stirring.

The 25mM Tris/HCl (pH=7.50) that adds 500ml then, and dialysed like this 9 hours at 4 ℃.Add other 250ml Tris buffer (seeing above) subsequently, and dialysed in addition 12 hours.

And then add the Tris/HCl (pH=7.50) of 500ml 25mM, and dialysed like this 9 hours at 4 ℃.Add other 250ml Tris buffer (seeing above) subsequently, and dialysed in addition 12 hours.

And then add the Tris/HCl (pH=7.50) of 500ml 25mM, and dialysed like this 9 hours at 4 ℃.The Dialysis tubing that will comprise dialysis solution is then put into the 25mM Tris+150mMNaCl (pH=7.50) of 2L.Dialysed once more 12 hours at 4 ℃ then.

Take out the inclusions in the Dialysis tubing then.

Method 2: dialysis continuously

The 8M urea liquid (Ni chelating eluent, HiTrap) in, with 10ml Cellytic IB (Sigma, the number of ordering No.C5236) and 5mM DTT processing 20ml KBD-B inclusion body.Pour solution into dialysate chamber then: Slide-A-Lyzer Dialyses Cassette PIERCE, MWCO:10kD.The number of ordering No.:66830.

Dialysed about 1 hour at 4 ℃ of 6M urea liquids then with 1L.

Then, during 48h in, by the following buffer of peristaltic pump continuous metering 2L: 25mMTris/HCl (pH=7.5).

In the Dialysis tubing that contains dialysis solution, add 2L stop buffer: 25mM Tris+150mM NaCl (pH=7.50) then, and dialysed about 12 hours at 4 ℃.

Take out the inclusions in the Dialysis tubing then.

Embodiment 8: with 1 (qualitative) that combine of skin

Developed visual qualitative test whether to study KBD in conjunction with skin.

Solutions employed:

Confining liquid: DIG washing liquid+be diluted in buffer series 1585762 BoehringerMA (10 * solution) among the TBS.

TBS：20mM?Tris；150mM?NaCl，pH?7.5

TTBS：TBS+0.05％Tween20

The first step is that the outer keratin of skin is transferred on the stable holder.For this purpose, adhesive tape is adhered to securely on the application on human skin of depilation, and take off once more.Can directly on adhesive tape, test, perhaps utilize new adhesion that adherent keratin layer is forwarded on the microscope slide.Following checking combination:

-in order to carry out incubation, change the Falcon pipe over to plurality of reagents;

If-be suitable for, add ethanol and carry out defat, remove ethanol and dry microscope slide;

Under-the room temperature with sealing buffer incubation 1h;

-wash 2 times each 5 minutes with TTBS;

-washed 1 time 5 minutes with TBS;

-in TBS/0.05%Tween 20, (be coupled to label, as His with KBD to be measured ₆, HA etc.) or control protein in room temperature incubation 2-4h;

-remove supernatant;

-wash 3 times with TBS;

-with in the TBS+0.01% confining liquid by 1: 2000 the dilution monoclonal anti polyhistidyl (or specific rabbit KBD) antibody in room temperature incubation 1h;

-wash 2 times each 5 minutes with TTBS;

-washed 1 time 5 minutes with TBS;

-with in the TBS+0.01% confining liquid by 1: 5000 the dilution anti-mice IgG alkali phosphatase conjugate in room temperature incubation 1h;

-wash 2 times each 5 minutes with TTBS;

-washed 1 time 5 minutes with TBS;

-adding phosphatase substrate (NBT-BCIP; 1/40ml of Boehringer MA water reacted 2.5 minutes; The water cessation reaction)

-with the naked eye or use Optics in Microscope to detect coloured precipitate.Blue precipitate represents that KBD has been incorporated into skin.

Embodiment 9: with 2 (quantitatively) that combine of skin

Developed quantitative test, available comparison nonspecific proteins matter and KBD be to the bond strength of hair/skin.

Use the 5mm card punch on skin that thaw, atrichous (people or pig) dry crushing sheet, to get out section (or with regard to surface test, skin biopsy being inserted in the Falcon slide glass).It is thick then skin samples to be processed into 2-3mm, to remove any tissue that exists.Then skin samples is changed over to Eppendorf pipe (the weak combination of protein) to carry out combination checking (referring to Fig. 7):

-wash 2 times with PBS/0.05%Tween 20;

-add the PBS solution of 1ml 1%BSA, and softly rotate (900rpm) incubation 1h in room temperature.

-remove supernatant.

-in PBS, add 100 μ g KBD with 0.05%Tween 20; Room temperature incubation 2h and soft rotate (900rpm).

-remove supernatant

-wash 3 times with PBS/0.05%Tween 20.

-with the peroxidase conjugated thing of 1ml monoclonal mouse anti label (His6 or HA or specificity KBD) antibody (be dissolved at 1: 2000 PBS) [the monoclonal anti polyhistidyl peroxidase conjugated thing lyophilized powder that in mice, prepares with 0.05%Tween 20, Sigma] in room temperature incubation 2-4h, soft rotate (900rpm).

-wash 3 times with PBS/0.05%Tween 20.

-(1ml/Eppendorf manages to add peroxidase substrate; Form and vide infra).

-allow reaction to proceed to (about 90 seconds) till the blue colour developing.

-with 100 μ l, 2 M H ₂SO ₄Cessation reaction.

-in 450nm measure light absorption value.

Peroxidase substrate (preparation newly in advance):

0.1ml TMB solution (the DMSO liquid of 42mM TMB)

+ 10ml substrate buffer solution (the 0.1M sodium acetate, pH4.9)

+ 14.7 μ l H ₂O ₂, concentration is 3%

Embodiment 10: with combine (quantitatively) of hair

In order to prove that KBD is also relevant with other albumen to the bond strength of hair, has developed quantitative determination process (referring to Fig. 7).In this test, at first with hair with the KBD incubation, and the excessive KBD of flush away.Pass through His label and the antibody-peroxidase conjugated thing coupling of KBD then.The unconjugated antibody of flush away-peroxidase conjugated thing once more.Bonded antibody-peroxidase conjugated thing [monoclonal anti polyhistidyl peroxidase conjugated thing, the lyophilized powder for preparing in mice, Sigma] can be converted into colourless substrate (TMB) coloured product that can carry out photometric measurement at the 405nm place.Photon absorbing intensity is represented the amount of bonded KBD or control protein.For example, control protein is selected from the YaaD of bacillus subtilis, its have equally [for this test necessary] the His label that is used to detect.Also can use the peroxidase conjugated thing of other specific antibody to replace the His label.

5mg hair (people) is cut into the long section of 5mm, and changes Eppendorf pipe (the weak combination of albumen) over to so that carry out the combination checking:

-adding is used for the 1ml ethanol of defat;

-centrifugal, remove ethanol and use H ₂The O washing;

-add the PBS solution of 1ml 1%BSA, and softly rotate incubation 1h in room temperature;

-centrifugal, remove supernatant;

-adding keratin binding structural domain (being coupled to label) or control protein to be measured in the PBS/0.05%Tween 20 of 1ml as His6, HA or the like; 4 ℃ of soft rotations with incubation 16h (or room temperature incubation 2h) at least;

-centrifugal, remove supernatant;

-wash 3 times with PBS/0.05%Tween 20;

-the peroxidase conjugated thing of 1ml monoclonal mouse anti label (His6 or HA or specificity KBD) antibody (be dissolved at 1: 2000 PBS/0.05%Tween 20) [the peroxidase conjugated thing lyophilized powder of the monoclonal anti polyhistidyl for preparing in mice, Sigma] is softly rotated in room temperature incubation 2-4h together;

-wash 3 times with PBS/0.05%Tween 30;

-adding peroxidase substrate (1ml/Eppendorf pipe);

-allow reaction to proceed to (about 2 minutes) till the blue colour developing;

-with the 2 M H of 100 μ l ₂SO ₄Cessation reaction.

-in 450nm measure light absorption value.

Peroxidase substrate (preparation newly in advance):

0.1ml TMB solution (the DMSO liquid of 42mM TMB);

+ 10ml substrate buffer solution (the 0.1M sodium acetate, pH4.9);

+ 14.7 μ l H ₂O ₂, concentration is 3%.

The BSA=bovine serum albumin;

The PBS=phosphate buffered saline;

Tween 20=polyoxyethylene mono laurate sorbitan ester, n about 20;

TMB=3,5,3 ', 5 '-the N tetramethyl benzidine.

What carry out on hair is the combination test of example with KBD-B, proves with the significantly weak combination of control protein YaaD to compare, and KBD-B has suitable advantage to the combination of hair:

1	Buffer	?A _405nm＝0.000
			?2	Control protein YaaD	?A _405nm＝0.088
?3	The KBD-B of degeneration	?A _405nm＝0.254
			?4	The KBD-B of renaturation	?A _405nm＝1.591

Table 1: the quantitative active testing of the KBD of hair: 1) buffer; 2) control protein YaaD; 3) KBD-B of degeneration; 4) KBD-B of renaturation.This table has shown the absorption value of surveying at 405nm.

Embodiment 10a: the coupling of dyestuff and KBD-B

For with fluorescent dye (Alexa Fluor 532, Molecular Probes/Invitrogen) and KBD-B albumen coupling, come the coupling dyestuff according to the maleic acid imidodicarbonic diamide joint of following method by the cysteine mercapto.Reaction as shown in Figure 8.

-1mg Alexa Fluor 532 is dissolved among the 150 μ l PBS buffer (pH=7.0); Of short duration subsequently centrifugal to remove any indissolvable component;

-the dissolved dyestuff of 10 μ l is added among the 100 μ g KBD-B (1mg/ml);

-use the aluminium foil covering mixture, and on agitator with 450pm in 24 ℃ of incubations 1 hour;

The 1 M DTT of-adding 10 μ l comes the maleic acid imidodicarbonic diamide functional group of the unreacted Alexa Fluor 532 of inactivation;

-then 450pm and 24 ℃ of incubations (covering) 30 minutes with aluminium foil.

By active testing (referring to embodiment 9 and 10), be measured to the coupling that coupling has KBD-B and the skin/hair of Alexa Fluor 532.Be similar to embodiment 9 or 10 and be combined in KBD-B-Alexa Fluor 532 couplings on skin or the hair, can be at an easy rate with fluorescence microscope (launch in absorption: 532nm/: the 590nm place is detected) on the hair or with the naked eye on the hair of bleaching, detect.

The effect of embodiment 11:KBD in oil-in-water type day nursing Emulsion

AI1％：

% composition (INCI)

A 1.7 ceteareth-6, octadecanol

0.7 ceteareth-25

2.0 diethylamino (2-hydroxybenzoyl) hexyl benzene formic acid esters

2.0 PEG-14 dimethicone

3.6 cetearyl alcohol

6.0 ethylhexyl Methoxycinnamate

2.0 dibutyl adipate

B 5.0 glycerol

0.2 sodium ethylene diamine tetracetate

1.0 pantothenylol

An amount of antiseptic

67.8 water

C 4.0 caprylic/capric triglyceride, sodium acrylate copolymer

D 0.2 vitamin C sodium phosphate

1.0 tocopheryl acetate

0.2 Bisabolol terpene alcohol

1.0 caprylic/capric triglyceride, sodium ascorbate, tocopherol, retinol

1.0 have the aqueous solution of about 5% keratin binding structural domain active component

An amount of sodium hydroxide of E

AI?5％：

% composition (INCI)

A 1.7 ceteareth-6, octadecanol

0.7 ceteareth-25

2.0 diethylamino (2-hydroxybenzoyl) hexyl benzene formic acid esters

2.0 PEG-14 dimethicone

3.6 cetearyl alcohol

6.0 basic hexyl Methoxycinnamate

2.0 dibutyl adipate

B 5.0 glycerol

0.2 sodium ethylene diamine tetracetate

1.0 pantothenylol

An amount of antiseptic

63.8 water

C 4.0 caprylic/capric triglyceride, sodium acrylate copolymer

D 0.2 vitamin C sodium phosphate

1.0 tocopheryl acetate

0.2 Bisabolol terpene alcohol

1.0 caprylic/capric triglyceride, sodium ascorbate, tocopherol, retinol

5.0 have the aqueous solution of about 5% keratin binding structural domain active component

An amount of sodium hydroxide of E

Preparation: heat A independent of one another mutually and extremely about 80 ℃ mutually of B.With B pour into while stirring mutually A mutually in, and stir evenly.C is mixed in the mixed phase of A and B mutually while stirring, and stirs evenly once more.Stirring is cooled to about 40 ℃, adds the D phase, regulates pH mutually to about 6.5 with E, stirs evenly, and stirs and be cooled to room temperature.

Attention: the preparation preparation need not to protect gas.Must in the packing of oxygen flow not, carry out fill, as aluminum pipe.

The purposes of embodiment 12:KBD in oil-in-water type protectiveness day cream

AI?1％：

% composition (INCI)

A 1.7 ceteareth-6, octadecanol

0.7 ceteareth-25

2.0 diethylamino (2-hydroxybenzoyl) hexyl benzene formic acid esters

2.0 PEG-14 dimethicone

3.6 cetearyl alcohol

6.0 basic hexyl Methoxycinnamate

2.0 dibutyl adipate

B 5.0 glycerol

0.2 sodium ethylene diamine tetracetate

1.0 pantothenylol

An amount of antiseptic

68.6 water

C 4.0 caprylic/capric triglyceride, sodium acrylate copolymer

D 1.0 vitamin C sodium phosphates

1.0 tocopheryl acetate

0.2 Bisabolol terpene alcohol

An amount of sodium hydroxide of E

AI?5％：

% composition (INCI)

A 1.7 ceteareth-6, octadecanol

0.7 ceteareth-25

2.0 diethylamino (2-hydroxybenzoyl) hexyl benzene formic acid esters

2.0 PEG-14 dimethicone

3.6 cetearyl alcohol

6.0 basic hexyl Methoxycinnamate

2.0 dibutyl adipate

B 5.0 glycerol

0.2 sodium ethylene diamine tetracetate

1.0 pantothenylol

An amount of antiseptic

64.6 water

C 4.0 caprylic/capric triglyceride, sodium acrylate copolymer

D 1.0 vitamin C sodium phosphates

1.0 tocopheryl acetate

0.2 Bisabolol terpene alcohol

An amount of sodium hydroxide of E

Preparation: heat A independent of one another mutually and extremely about 80 ℃ mutually of B.With B pour into while stirring mutually A mutually in, and stir evenly.C is poured into mutually in the mixed phase of A and B, and stir evenly.Stirring is cooled to about 40 ℃.Add the D phase, regulate pH mutually to about 6.5 and stir evenly with E.Stirring is cooled to room temperature.The purposes of embodiment 13:KBD in clean lotion of oil-in-water type

AI?1％：

% composition (INCI)

A 10.0 cetearyl ethylhexoates

10.0 caprylic/capric triglyceride

1.5 encircle penta siloxanes, hexamethylene siloxanes

2.0 PEG-40 castor oil hydrogenated

B 3.5 caprylic/capric triglyceride, sodium acrylate copolymer

C 1.0 tocopheryl acetates

0.2 Bisabolol terpene alcohol

An amount of antiseptic

An amount of aromatic oil

D 3.0 polyquaternary ammonium salt-44

0.5 cocos nucifera oil trimethyl sulfate methyl ammonium

0.5 ceteareth-25

2.0 pantothenylol, propylene glycol

4.0 propylene glycol

0.1 sodium ethylene diamine tetracetate

60.7 water

AI?5％：

% composition (INCI)

A 10.0 cetearyl ethylhexoates

10.0 caprylic/capric triglyceride

1.5 encircle penta siloxanes, hexamethylene siloxanes

2.0 PEG-40 castor oil hydrogenated

B 3.5 caprylic/capric triglyceride, sodium acrylate copolymer

C 1.0 tocopheryl acetates

0.2 Bisabolol terpene alcohol

An amount of antiseptic

An amount of aromatic oil

D 3.0 polyquaternary ammonium salt-44

0.5 cocos nucifera oil trimethyl sulfate methyl ammonium

0.5 ceteareth-25

2.0 pantothenylol, propylene glycol

4.0 propylene glycol

0.1 sodium ethylene diamine tetracetate

56.7 water

Preparation: dissolving A phase.With B pour into while stirring mutually A mutually in.C is poured into mutually in the mixed phase of A and B.Dissolving D phase is poured into while stirring in the mixed phase of A, B and C, and is stirred evenly.Stirred afterwards 15 minutes.

The purposes of embodiment 14:KBD in the daily nursing body sprays

AI?1％：

% composition (INCI)

A 3.0 basic hexyl Methoxycinnamates

2.0 diethylamino (2-hydroxybenzoyl) hexyl benzene formic acid esters

1.0 polyquaternary ammonium salt-44

3.0 propylene glycol

2.0 pantothenylol, propylene glycol

1.0 encircle penta siloxanes, hexamethylene siloxanes

10.0 octyl dodecanol

0.5 PVP

10.0 caprylic/capric triglyceride

3.0 C12-15 alkyl benzoate

3.0 glycerol

1.0 tocopheryl acetate

0.3 Bisabolol terpene alcohol

59.2 alcohol

AI 5％：

% composition (INCI)

A 3.0 basic hexyl Methoxycinnamates

20 diethylamino (2-hydroxybenzoyl) hexyl benzene formic acid esters

1.0 polyquaternary ammonium salt-44

3.0 propylene glycol

2.0 pantothenylol, propylene glycol

1.0 encircle penta siloxanes, hexamethylene siloxanes

10.0 octyl dodecanol

0.5 PVP

10.0 caprylic/capric triglyceride

3.0 C12-15 alkyl benzoate

3.0 glycerol

1.0 tocopheryl acetate

0.3 Bisabolol terpene alcohol

55.2 alcohol

Preparation: weigh and dissolve the A phase component until clarification.

The purposes of embodiment 15:KBD in the skin nursing gel

AI 1％：

% composition (INCI)

A 3.6 PEG-40 castor oil hydrogenated

15.0 alcohol

0.1 Bisabolol terpene alcohol

0.5 tocopheryl acetate

An amount of aromatic oil

B 3.0 pantothenylol

0.6 carbomer

75.4 water

C 0.8 triethanolamine

AI?5％：

% composition (INCI)

A 3.6 PEG-40 castor oil hydrogenated

15.0 alcohol

0.1 Bisabolol terpene alcohol

0.5 tocopheryl acetate

An amount of aromatic oil

B 3.0 pantothenylol

0.6 carbomer

71.4 water

C 0.8 triethanolamine

Preparation: A is up to clarification in dissolving.Make B expand mutually and neutralize mutually with C.With A pour into while stirring mutually uniform B mutually in, and stir evenly.

The purposes of embodiment 16:KBD in the back maintenance liquid that shaves

AI?1％：

% composition (INCI)

A 10.0 cetearyl ethylhexoates

5.0 tocopheryl acetate

1.0 Bisabolol terpene alcohol

0.1 aromatic oil

0.3 acrylate/C10-30 alkyl acrylate cross-linked polymer

B 15.0 alcohol

1.0 pantothenylol

3.0 glycerol

0.1 triethanolamine

63.5 water

AI?5％：

% composition (INCI)

A 10.0 cetearyl ethylhexoates

5.0 tocopheryl acetate

1.0 Bisabolol terpene alcohol

0.1 aromatic oil

0.3 acrylate/C10-30 alkyl acrylate cross-linked polymer

B 15.0 alcohol

1.0 pantothenylol

3.0 glycerol

0.1 triethanolamine

59.5 water

Preparation: the component of mixing the A phase.Dissolving B phase, be incorporated into A mutually in and stir evenly.

Embodiment 17:KBD is the purposes in the lotion after Exposure to Sunlight

AI?1％：

% composition (INCI)

A 0.4 acrylate/C10-30 alkyl acrylate cross-linked polymer

15.0 cetearyl ethylhexoate

0.2 Bisabolol terpene alcohol

1.0 tocopheryl acetate

An amount of aromatic oil

B 1.0 pantothenylol

15.0 alcohol

3.0 glycerol

63.2 water

C 0.2 triethanolamine

AI?5％：

% composition (INCI)

A 0.4 acrylate/C10-30 alkyl acrylate cross-linked polymer

15.0 cetearyl ethylhexoate

0.2 Bisabolol terpene alcohol

1.0 tocopheryl acetate

An amount of aromatic oil

B 1.0 pantothenylol

15.0 alcohol

3.0 glycerol

59.2 water

C 0.2 triethanolamine

Preparation: the component of mixing the A phase.With B pour into while stirring mutually A mutually in.Neutralize mutually and stir evenly once more with C.

The purposes of embodiment 18:KBD in sunscreen lotion

AI?1％：

% composition (INCI)

A 4.5 basic hexyl Methoxycinnamates

2.0 diethylamino (2-hydroxybenzoyl) hexyl benzene formic acid esters

3.0 1-Octyl acrylate

2.5 two C12-13 alkyl malates

0.5 tocopheryl acetate

4.0 polyglycereol-3 methyl glucoside distearate

B 3.5 different n-nonanoic acid cetearyl alcohol esters

1.0 VP/ icosane copolymer

5.0 2-Methylpentadecane

2.5 two C12-13 alkyl malates

30 titanium dioxide, trimethoxy decoyl silane

C 5.0 glycerol

1.0 cetearyl alcohol sodium sulfate

0.5 xanthan gum

59.7 water

D 1.0 has the aqueous solution of about 5% keratin binding structural domain active component

1.0 phenoxyethanol, methyl parahydroxybenzoate, ethylparaben,

Butyl p-hydroxybenzoate, propyl parabene, p-Hydroxybenzoic acid isobutyl ester

0.3 Bisabolol terpene alcohol

AI?5％：

% composition (INCI)

A 4.5 basic hexyl Methoxycinnamates

2.0 diethyl base ammonia (2-hydroxybenzoyl) hexyl benzene formic acid esters

3.0 1-Octyl acrylate

2.5 two C12-13 alkyl malates

0.5 tocopheryl acetate

4.0 polyglycereol-3 methyl glucoside distearate

B 3.5 different n-nonanoic acid cetearyl alcohol esters

1.0 VP/ icosane copolymer

5.0 2-Methylpentadecane

2.5 two C12-13 alkyl malic acids

3.0 titanium dioxide, trimethoxy decoyl silane

C 5.0 glycerol

1.0 cetearyl alcohol sodium sulfate

0.5 xanthan gum

55.7 water

D 5.0 has the aqueous solution of about 5% keratin binding structural domain active component

1.0 phenoxyethanol, methyl parahydroxybenzoate, ethylparaben,

Butyl p-hydroxybenzoate, propyl parabene, p-Hydroxybenzoic acid isobutyl ester

0.3 Bisabolol terpene alcohol

Preparation: heat A phase and extremely about 80 ℃ of B components mutually independent of one another.With B pour into while stirring mutually A mutually in, and stir evenly.To about 80 ℃, stirring is poured the mixed phase of A and B into and is stirred evenly with the C heat phase.Stirring is cooled to about 40 ℃, adds D and also stirs evenly once more mutually.

The purposes of embodiment 19:KBD in the oil-in-water type sunscreen lotion

AI?1％：

% composition (INCI)

A 2.0 ceteareth-6, octadecanol

2.0 ceteareth-25

3.0 methyl behenate

2.0 cetearyl alcohol

2.0 cetearyl ethylhexoate

5.0 ethylhexyl Methoxycinnamate

1.0 basic hexyl triazinone

1.0 VP/ icosane copolymer

7.0 isopropyl myristate

B 5.0 zinc oxides, the sad silane of triethoxy

C 0.2 xanthan gum

0.5 hydroxy ethyl methacrylate/sodium acryloyldimethyl taurate copolymers, squalane,

Polysorbate 60

0.2 sodium ethylene diamine tetracetate

5.0 propylene glycol

0.5 pantothenylol

60.9 water

0.5 phenoxyethanol, methyl parahydroxybenzoate, ethylparaben,

Ethylparaben, propyl parabene, p-Hydroxybenzoic acid isopropyl ester

1.0 tocopheryl acetate

0.2 Bisabolol terpene alcohol

AI?5％：

% composition (INCI)

A 2.0 ceteareth-6, octadecanol

2.0 ceteareth-25

3.0 methyl behenate

2.0 cetearyl alcohol

2.0 cetearyl ethylhexoate

5.0 ethylhexyl Methoxycinnamate

1.0 basic hexyl triazinone

1.0 VP/ icosane copolymer

7.0 isopropyl myristate

B 5.0 zinc oxides, the sad silane of triethoxy

C 0.2 xanthan gum

Polysorbate 60

0.2 sodium ethylene diamine tetracetate

5.0 propylene glycol

0.5 pantothenylol

56.9 water

0.5 phenoxyethanol, methyl parahydroxybenzoate, ethylparaben,

Ethylparaben, propyl parabene, p-Hydroxybenzoic acid isopropyl ester

1.0 tocopheryl acetate

0.2 Bisabolol terpene alcohol

Prepare: heating A is mutually to about 80 ℃, and stirring is poured the B phase into and stirred evenly 3 minutes.Similarly heat C to 80 ℃, stirring is poured in A and the B mixed phase and is stirred evenly.Be cooled to about 40 ℃, stirring is poured D into and is also stirred evenly once more mutually

The purposes of embodiment 20:KBD in the oil-in-water type sunscreen lotion

AI?1％：

% composition (INCI)

A 3.5 ceteareth-6, octadecanol

1.5 ceteareth-25

7.5 ethylhexyl Methoxycinnamate

2.0 diethylamino (2-hydroxybenzoyl) hexyl benzene formic acid esters

2.0 encircle penta siloxanes, hexamethylene siloxanes

0.5 Cera Flava

3.0 cetearyl alcohol

10.0 caprylic/capric triglyceride

B 5.0 titanium dioxide, Silicon stone, methyl-silicone oil, Alumina

C 3.0 glycerol

0.2 sodium ethylene diamine tetracetate

0.3 xanthan gum

1.0 decyl glucosides

2.0 pantothenylol, propylene glycol

56.3 water

1.0 tocopheryl acetate

0.2 Bisabolol terpene alcohol

An amount of aromatic oil

An amount of antiseptic

AI?5％：

% composition (INCI)

A 3.5 ceteareth-6, pantothenylol

1.5 ceteareth-25

7.5 ethylhexyl Methoxycinnamate

2.0 diethylamino (2-hydroxybenzoyl) hexyl benzene formic acid esters

2.0 encircle penta siloxanes, hexamethylene siloxanes

0.5 Cera Flava

3.0 cetearyl alcohol

10.0 caprylic/capric triglyceride

B 5.0 titanium dioxide, Silicon stone, methyl-silicone oil, Alumina

C 3.0 glycerol

0.2 sodium ethylene diamine tetracetate

0.3 xanthan gum

1.0 decyl glucosides

2.0 pantothenylol, propylene glycol

52.3 water

1.0 tocopheryl acetate

0.2 Bisabolol terpene alcohol

An amount of aromatic oil

An amount of antiseptic

Prepare: heating A is mutually to about 80 ℃, and stirring is poured the B phase into and stirred evenly 3 minutes.Similarly, heat C to 80 ℃, stir in the mixed phase pour A and B into and stir evenly.Be cooled to about 40 ℃, stirring is poured D into and is also stirred evenly once more mutually.

The purposes of embodiment 21:KBD in the foot balsam

AI?1％：

% composition (INCI)

A 2.0 ceteareth-6, pantothenylol

2.0 ceteareth-25

5.0 cetearyl ethylhexoate

4.0 spermol

4.0 tristerin

5.0 mineral oil

0.2 menthol

0.5 Camphora

B 69.3 water

An amount of antiseptic

C 1.0 Bisabolol terpene alcohol

1.0 tocopheryl acetate

5.0 Radix Hamamelidis Mollis extract

AI?5％：

% composition (INCI)

A 2.0 ceteareth-6, pantothenylol

2.0 ceteareth-25

5.0 cetearyl ethylhexoate

4.0 spermol

4.0 tristerin

5.0 mineral oil

0.2 menthol

0.5 Camphora

B 65.3 water

An amount of antiseptic

C 1.0 Bisabolol terpene alcohol

1.0 tocopheryl acetate

5.0 Radix Hamamelidis Mollis extract

Preparation: heat A phase and extremely about 80 ℃ of B components mutually independent of one another.With B stir mutually pour into A mutually in.Stirring is cooled to about 40 ℃, of short duration stir evenly the back add C mutually with D mutually.Stirring is cooled to room temperature.

The purposes of embodiment 22:KBD in water-in-oil emulsion with Bisabolol terpene alcohol

AI?1％：

% composition (INCI)

A 6.0 PEG-7 castor oil hydrogenated

8.0 cetearyl ethylhexoate

5.0 isopropyl myristate

15.0 mineral oil

0.3 magnesium stearate

0.3 aluminium stearate

2.0 PEG-45/ dodecyl glycol copolymer

B 5.0 glycerol

0.7 magnesium sulfate

55.6 water

C 1.0 has the aqueous solution of about 5% keratin binding structural domain active component

0.5 tocopheryl acetate

0.6 Bisabolol terpene alcohol

AI?5％：

% composition (INCI)

A 6.0 PEG-7 castor oil hydrogenated

8.0 cetearyl ethylhexoate

5.0 isopropyl myristate

15.0 mineral oil

0.3 magnesium stearate

0.3 aluminium stearate

2.0 PEG-45/ dodecyl glycol copolymer

B 5.0 glycerol

0.7 magnesium sulfate

51.6 water

C 5.0 has the aqueous solution of about 5% keratin binding structural domain active component

0.5 tocopheryl acetate

Preparation: heat A independent of one another mutually and extremely about 85 ℃ mutually of B.With B stir mutually pour into A mutually in, and stir evenly.Stirring is cooled to about 40 ℃, adds C phase and of short duration once more stirring evenly.Stirring is cooled to room temperature.

Preparation tabulation-the hair care that is used for patent keratin binding structural domain

Embodiment 23: the foamed glue conditioner with setting agent

AI?1％

% composition (INCI)

A 10.0 PVP/VA copolymers

0.2 ethoxy spermaceti dimethyl ammonium phosphate

0.2 ceteareth-25

0.5 dimethicone copolyol

An amount of aromatic oil

10.0 alcohol

68.1 water

10.0 propane/butane

AI?5％

% composition (INCI)

A 10.0 PVP/VA copolymers

0.2 ethoxy spermaceti dimethyl ammonium phosphate

0.2 ceteareth-25

0.5 dimethicone copolyol

An amount of aromatic oil

10.0 alcohol

64.1 water

10.0 propane/butane

Preparation: the component of the A phase of weighing together, stir until all the components dissolving and fill.

Embodiment 24: the foamed glue conditioner

AI?1％

% composition (INCI)

A 1.0 polyquaternary ammonium salt-4

0.5 ethoxy spermaceti dimethyl ammonium phosphate

An amount of aromatic oil

An amount of antiseptic

91.5 water

6.0 propane/butane

AI?5％

% composition (INCI)

A 1.0 polyquaternary ammonium salt-4

0.5 ethoxy spermaceti dimethyl ammonium phosphate

An amount of aromatic oil

An amount of antiseptic

87.5 water

6.0 propane/butane

Preparation: the component of the A phase of weighing together, stir until all the components dissolving and produce clear liquid and fill.

Embodiment 25: the foamed glue conditioner

AI?1％

% composition (INCI)

A 1.0 polyquaternary ammonium salt-11

0.5 ethoxy spermaceti dimethyl ammonium phosphate

An amount of aromatic oil

An amount of antiseptic

91.5 water

6.0 propane/butane

AI?5％

% composition (INCI)

A 1.0 polyquaternary ammonium salt-11

0.5 ethoxy spermaceti dimethyl ammonium phosphate

An amount of aromatic oil

An amount of antiseptic

87.5 water

6.0 propane/butane

Embodiment 26: the hair style foamed glue

AI?1?％

% composition (INCI)

A 0.5 lauryl alcohol-4

An amount of aromatic oil

B 77.3 water

10.0 polyquaternary ammonium salt-28

0.5 dimethicone copolyol

0.2 ceteareth-25

0.2 pantothenylol

0.1 PEG-25?PABA

0.2 hydroxyethyl-cellulose

C 10.0 HFC?152?A

AI 5％

% composition (INCI)

A 0.5 lauryl alcohol-4

An amount of aromatic oil

B 73.3 water

10.0 polyquaternary ammonium salt-28

0.5 methyl-silicone oil copolyol

0.2 ceteareth-25

0.2 pantothenylol

0.1 PEG-25?PABA

0.2 hydroxyethyl-cellulose

C 10.0 HFC?152?A

Preparation: the component of mixing the A phase.The component and the dissolving that add the B phase one by one.With C fill together mutually.

Embodiment 27: the hair style foamed glue

AI?1％

% composition (INCI)

A 2.0 cocos nucifera oil trimethyl sulfate methyl ammoniums

An amount of aromatic oil

B 78.5 water

6.7 acrylate copolymer

0.6 AMP

0.5 dimethicone copolyol

0.2 ceteareth-25

0.2 pantothenylol

0.1 PEG-25?PABA

0.2 hydroxyethyl-cellulose

C 10.0 HFC?152?A

AI?5％

% composition (INCI)

A 2.0 cocos nucifera oil trimethyl sulfate methyl ammoniums

An amount of aromatic oil

B 74.5 water

6.7 acrylate copolymer

0.6 AMP

0.5 dimethicone copolyol

0.2 ceteareth-25

0.2 pantothenylol

0.1 PEG-25?PABA

0.2 hydroxyethyl-cellulose

C?10.0 HFC?152?A

Embodiment 28: the hair style foamed glue

AI?1％

% composition (INCI)

A 2,0 cocos nucifera oil trimethyl sulfate methyl ammoniums

An amount of aromatic oil

B 7.70 polyquaternary ammonium salt-44

An amount of antiseptic

79.3 water

C 10.0 propane/butane

AI?5％

% composition (INCI)

A 2.0 cocos nucifera oil trimethyl sulfate methyl ammoniums

An amount of aromatic oil

B 7.70 polyquaternary ammonium salt-44

An amount of antiseptic

75.3 water

C 10.0 propane/butane

Preparation: the component of mixing the A phase.Dissolving B phase component stirs B mutually then and pours the A phase into until clarification.Regulate pH to 6-7, with C fill together mutually.

Embodiment 29: the hair style foamed glue

AI?1％

% composition (INCI)

A 2.00 cocos nucifera oil trimethyl sulfate methyl ammoniums

An amount of aromatic oil

B 72.32 water

2.00 VP/ acrylate/metering system dodecyl gallate copolymer

0.53 AMP

1.00 have the aqueous solution of about 5% keratin binding structural domain active component

0.20 ceteareth-25

0.50 pantothenylol

0.05 benzophenone-4

0.20 amino dimethicone, hexadecyltrimethylammonium chloride,

Tridecyl polyoxyethylene ether-12

15.00 alcohol

C 0.20 hydroxyethyl-cellulose

D 6.00 propane/butane

AI?5％

% composition (INCI)

A 2.00 cocos nucifera oil trimethyl sulfate methyl ammoniums

An amount of aromatic oil

B 68.32 water

2.00 VP/ acrylate/metering system dodecyl gallate copolymer

0.53 AMP

5.00 have the aqueous solution of about 5% keratin binding structural domain active component

0.20 ceteareth-25

0.50 pantothenylol

0.05 benzophenone-4

0.20 amino dimethicone, hexadecyltrimethylammonium chloride,

Tridecyl polyoxyethylene ether-12

15.00 alcohol

C 0.20 hydroxyethyl-cellulose

D 6.00 propane/butane

Preparation: the component of mixing the A phase.The component and the dissolving that add the B phase one by one.C is dissolved in A and the B mixed phase mutually, regulates pH to 6-7 then.With D fill together mutually.

Embodiment 30: the hair style foamed glue

AI?1％

% composition (INCI)

A 2.00 hexadecyltrimethylammonium chlorides

An amount of aromatic oil

B 67.85 water

7.00 polyquaternary ammonium salt-46

0.20 ceteareth-25

0.50 pantothenylol

0.05 benzophenone-4

0.20 amino dimethicone, hexadecyltrimethylammonium chloride,

Tridecyl polyoxyethylene ether-12

15.00 alcohol

C 0.20 hydroxyethyl-cellulose

D 6.00 propane/butane

AI?5％

% composition (INCI)

A 2.00 hexadecyltrimethylammonium chlorides

An amount of aromatic oil

B 63.85 water

7.00 polyquaternary ammonium salt-46

0.20 ceteareth-25

0.50 pantothenylol

0.05 benzophenone-4

0.20 amino dimethicone, hexadecyltrimethylammonium chloride,

Tridecyl polyoxyethylene ether-12

15.00 alcohol

C 0.20 hydroxyethyl-cellulose

D 6.00 propane/butane

Embodiment 31: the hair style foamed glue

AI?1％

% composition (INCI)

A appropriate amount of PEG-40 castor oil hydrogenated

An amount of aromatic oil

85.5 water

B 7.0 kayexalates

0.5 cetyl trimethyl ammonium bromide

An amount of antiseptic

C 6.0 propane/butane

The hair style foamed glue

AI?5％

% composition (INCI)

A appropriate amount of PEG-40 castor oil hydrogenated

An amount of aromatic oil

81.5 water

B 7.0 kayexalates

0.5 cetyl trimethyl ammonium bromide

An amount of antiseptic

C 6.0 propane/butane

Preparation: dissolving A phase.Take by weighing B be added to A mutually in, and the dissolving until clarification.Regulate pH to 6-7, with C fill together mutually.

Embodiment 32: the hair style foamed glue

AI?1％

% composition (INCI)

A appropriate amount of PEG-40 castor oil hydrogenated

An amount of aromatic oil

92.0 water

B 0.5 polyquaternary ammonium salt-10

0.5 cetyl trimethyl ammonium bromide

An amount of antiseptic

C 6.0 propane/butane

AI?5％

% composition (INCI)

A appropriate amount of PEG-40 castor oil hydrogenated

An amount of aromatic oil

88.0 water

B 0.5 polyquaternary ammonium salt-10

0.5 cetyl trimethyl ammonium bromide

An amount of antiseptic

C 6.0 propane/butane

Embodiment 32: the hair style foamed glue

AI?1％

% composition (INCI)

A appropriate amount of PEG-40 castor oil hydrogenated

An amount of aromatic oil

82.5 water

B 10.0 polyquaternary ammonium salt-16

0.5 ethoxy spermaceti dimethyl ammonium phosphate

An amount of antiseptic

C 6.0 propane/butane

AI?5％

% composition (INCI)

A appropriate amount of PEG-40 castor oil hydrogenated

An amount of aromatic oil

78.5 water

B 10.0 polyquaternary ammonium salt-16

0.5 ethoxy spermaceti dimethyl ammonium phosphate

An amount of antiseptic

C 6.0 propane/butane

Embodiment 33: the hair style foamed glue

AI?1％

% composition (INCI)

A 2.0 cocos nucifera oil trimethyl sulfate methyl ammoniums

An amount of aromatic oil

B 84.0 water

2.0 chitosan

0.5 dimethicone copolyol

0.2 ceteareth-25

0.2 pantothenylol

0.1 PEG-25?PABA

C 10.0?HFC?152?A

AI?5％

% composition (INCI)

A 2.0 cocos nucifera oil trimethyl sulfate methyl ammoniums

An amount of aromatic oil

B 80.0 water

2.0 chitosan

0.5 dimethicone copolyol

0.2 ceteareth-25

0.2 pantothenylol

0.1 PEG-25?PABA

C 10.0?HFC?152?A

Embodiment 34: nursing shampoo

AI?1％

% composition (INCI)

A 30.0 lauryl alcohol sodium sulfate

6.0 cocos nucifera oil acyl both sexes guanidine-acetic acid sodium

6.0 cocoamidopropyl

3.0 lauryl alcohol sodium sulfate, glycol distearate, coconut oleoyl amine MEA,

Lauryl alcohol-10

7.7 polyquaternary ammonium salt-44

2.0 amino dimethicone

An amount of aromatic oil

An amount of antiseptic

1.0 sodium chloride

43.3 water

An amount of citric acid of B

AI?5％

% composition (INCI)

A 30.0 lauryl alcohol sodium sulfate

6.0 cocos nucifera oil acyl both sexes guanidine-acetic acid sodium

6.0 cocoamidopropyl

3.0 lauryl alcohol sodium sulfate, glycol distearate, coconut oleoyl amine MEA,

Lauryl alcohol-10

7.7 polyquaternary ammonium salt-44

2.0 amino dimethicone

An amount of aromatic oil

An amount of antiseptic

1.0 sodium chloride

39.3 water

An amount of citric acid of B

Preparation: mix the component and the dissolving of A phase.With the Fructus Citri Limoniae acid for adjusting pH to 6-7.

Embodiment 35: shower lotion

AI?1％

% composition (INCI)

A 40.0 lauryl alcohol sodium sulfate

5.0 decyl glucosides

5.0 cocoamidopropyl

1.0 pantothenylol

An amount of aromatic oil

An amount of antiseptic

2.0 sodium chloride

46.0 water

An amount of citric acid of B

AI?5％

% composition (INCI)

A 40.0 lauryl alcohol sodium sulfate

5.0 decyl glucosides

5.0 cocoamidopropyl

1.0 pantothenylol

An amount of aromatic oil

An amount of antiseptic

2.0 sodium chloride

42.0 water

An amount of citric acid of B

Preparation: the component of mixing the A phase.With the Fructus Citri Limoniae acid for adjusting pH to 6-7.

Embodiment 36: shampoo

AI?1％

% composition (INCI)

A 40.0 lauryl alcohol sodium sulfate

5.0 C12-15 Petiolus Trachycarpi oil ether-15 sodium sulfonate

5.0 decyl glucosides

An amount of aromatic oil

0.1 phytantriol

44.6 water

0.3 polyquaternary ammonium salt-10

1.0 pantothenylol

An amount of antiseptic

1.0 lauryl alcohol-3

2.0 sodium chloride

AI?5％

% composition (INCI)

A 40.0 lauryl alcohol sodium sulfate

5.0 C12-15 Petiolus Trachycarpi oil ether-15 sodium sulfonate

5.0 decyl glucosides

An amount of aromatic oil

0.1 phytantriol

40.6 water

0.3 polyquaternary ammonium salt-10

1.0 pantothenylol

An amount of antiseptic

1.0 lauryl alcohol-3

2.0 sodium chloride

Embodiment 37: shampoo

AI?1％

% composition (INCI)

A 15.00 cocoamidopropyl

10.00 cocos nucifera oil both sexes two acetic acid disodiums

5.00 poly mountain alcohol ester 20

5.00 decyl glucosides

An amount of aromatic oil

An amount of antiseptic

0.15 guar gum hydroxypropyl-trimethyl ammonium chloride

2.00 lauryl alcohol-3

58.00 water

An amount of citric acid

B 3.00 PEG-150 distearates

AI?5％

% composition (INCI)

A 15.00 cocoamidopropyl

10.00 cocos nucifera oil both sexes two acetic acid disodiums

5.00 poly mountain alcohol ester 20

5.00 decyl glucosides

An amount of aromatic oil

An amount of antiseptic

0.15 guar gum hydroxypropyl-trimethyl ammonium chloride

2.00 lauryl alcohol-3

54.00 water

An amount of citric acid

B 3.00 PEG-150 distearates

Preparation: weigh and dissolve the component of A phase.Regulate pH to 6-7.Add the B phase and be heated to about 50 ℃.Stirring is cooled to room temperature.

Embodiment 38: the watch box moisturiser

AI?1％

% composition (INCI)

A 2.0 ceteareth-25

2.0 ceteareth-6, pantothenylol

3.0 cetearyl ethylhexoate

1.0 dimethicone

4.0 cetearyl alcohol

3.0 tristerin SE

5.0 mineral oil

4.0 jojoba seed oil

3.0 mineral oil, lanolin alcohol

B 5.0 propylene glycol

1.0 pantothenylol

0.5 aluminium-magnesium silicate

An amount of antiseptic

65.5 water

An amount of aromatic oil of C

An amount of citric acid of D

AI5％

% composition (INCI)

A 2.0 ceteareth-25

2.0 ceteareth-6, pantothenylol

3.0 cetearyl ethylhexoate

1.0 dimethicone

4.0 cetearyl alcohol

3.0 tristerin SE

5.0 mineral oil

4.0 jojoba seed oil

3.0 mineral oil, lanolin alcohol

B 5.0 propylene glycol

1.0 pantothenylol

0.5 aluminium-magnesium silicate

An amount of antiseptic

61.5 water

An amount of aromatic oil of C

An amount of citric acid of D

Preparation: heat A separately mutually and extremely about 80 ℃ mutually of B.The of short duration B phase that stirs evenly in advance stirs B then and pours A mutually into mutually and stir evenly once more.Be cooled to about 40 ℃, add C and also thoroughly stir evenly once more mutually.With the Fructus Citri Limoniae acid for adjusting pH to 6-7.

Embodiment 39: the watch box moisturiser

AI1％

% composition (INCI)

A 6.0 PEG-7 castor oil hydrogenated

10.0 cetearyl ethylhexoate

5.0 isopropyl myristate

7.0 mineral oil

0.5 Adeps Bovis seu Bubali resin (Butyrospermum)

0.5 aluminium stearate

0.5 magnesium stearate

0.2 Bisabolol terpene alcohol

0.7 quaternary ammonium salt-18-hectorite

B 5.0 dipropylene glycol

0.7 magnesium sulfate

An amount of antiseptic

62.9 water

An amount of aromatic oil of C

AI5％

% composition (INCI)

A 6.0 PEG-7 castor oil hydrogenated

10.0 cetearyl ethylhexoate

5.0 isopropyl myristate

7.0 mineral oil

0.5 Adeps Bovis seu Bubali resin (Butyrospermum)

0.5 aluminium stearate

0.5 magnesium stearate

0.2 Bisabolol terpene alcohol

0.7 quaternary ammonium salt-18-hectorite

B 5.0 dipropylene glycol

0.7 magnesium sulfate

An amount of antiseptic

58.9 water

An amount of aromatic oil of C

Preparation: heat A individually mutually and extremely about 80 ℃ mutually of B.With B stir mutually pour into A mutually in, and stir evenly.Stirring is cooled to about 40 ℃, adds C and also stirs evenly once more mutually.Stirring is cooled to room temperature.Embodiment 40: the liquid make-up of oil-in-water type

AI?1％

% composition (INCI)

A 2.0 ceteareth-6, pantothenylol

2.0 ceteareth-25

6.0 tristerin

1.0 spermol

8.0 mineral oil

7.0 cetearyl ethylhexoate

0.2 dimethicone

B 3.0 propylene glycol

1.0 pantothenylol

An amount of antiseptic

61.9 water

C 0.1 Bisabolol terpene alcohol

An amount of aromatic oil

D 5.7 C.I.77 891, titanium dioxide

1.1 iron oxides

AI5％

% composition (INCI)

A 2.0 ceteareth-6, pantothenylol

2.0 ceteareth-25

6.0 tristerin

1.0 spermol

8.0 mineral oil

7.0 cetearyl ethylhexoate

0.2 dimethicone

B 3.0 propylene glycol

1.0 pantothenylol

An amount of antiseptic

57.9 water

C 0.1 Bisabolol terpene alcohol

An amount of aromatic oil

D 5.7 C.I.77 891, titanium dioxide

1.1 iron oxides

Preparation: heat A individually mutually and extremely about 80 ℃ mutually of B.With B stir mutually pour into A mutually in, and stir evenly.Stirring is cooled to about 40 ℃, adds C and also thoroughly stirs evenly once more mutually with D mutually.Stirring is cooled to room temperature.

Embodiment 41

In following exemplary preparation, the active component of employing is 5% of keratin binding structural domain aqueous solution or keratin-binding effector molecules aqueous solution by weight.Following data are weight ratio parts.

The cleaning shampoo

Composition (INCI)	?1	?2	?3	?4	?5
						Texapon?N?70	?13.00	?15.00	?10.50	?12.50	?10.00
Dehyaufn?PK?45	?7.50	?7.00	?5.00	?5.50	?10.00
						Cetiol?HE	?2.00	?2.50	?3.50	?5.00	?2.30
Fragrance	?0.10	?0.10	?0.10	?0.10	?0.10
						Aqueous solution with keratin binding structural domain active component	?1.0	?5.0	?0.1	?0.5	?10.0
D-panthenol USP	?1.00	?1.50	?1.80	?1.70	?1.40
						Antiseptic	?0.10	?0.10	?0.10	?0.10	?0.10
Citric acid	?0.10	?0.10	?0.10	?0.10	?0.10
						Luviquat?Ultra?Care	?1.50	?1.00	?1.50	?1.20	?1.10
Sodium chloride	?1.50	?1.40	?1.40	?1.30	?1.50
						Water	Add to 100	Add to 100	Add to 100	Add to 100	Add to 100

Shampoo

Composition (INCI)	?1	?2	?3	?4	?5
						Texapon?NSO	?35.00	?40.00	?30.00	?45.00	?27.00
Plantacare?2000	?5.00	?5.50	?4.90	?3.50	?7.00
						Tego betanin L7	?10.00	?5.00	?12.50	?7.50	?15.00
Fragrance	?0.10	?0.10	?0.10	?0.10	?0.10
						Aqueous solution with keratin binding structural domain active component	?1.0	?5.0	?0.1	?0.5	?10.0
D-panthenol USP	?0.50	?1.00	?0.80	?1.50	?0.50

Antiseptic	0.10	?0.10	?0.10	?0.10	?0.10
						Citric acid	0.10	?0.10	?0.10	?0.10	?0.10
Rewopal?LA?3	0.50	?2.00	?0.50	?0.50	?2.00
						Sodium chloride	1.50	?1.50	?1.50	?1.50	?1.50
Water	Add to 100	Add to 100	Add to 100	Add to 100	Add to 100

The cleaning conditioner shampoo

Composition (INCI)	?1	?2	?3	?4	?5
						Amphotensid?GB?2009	?10.00	?15.00	?20.00	?12.00	?17.00
Plantacare?2000	?5.00	?6.00	?7.00	?8.00	?4.00
						Tego betanin L7	?15.00	?12.00	?10.00	?18.00	?20.00
Luviquat?FC?550	?0.30	?0.20	?0.20	?0.20	?0.30
						Fragrance	?0.10	?0.10	?0.10	?0.10	?0.10
Aqueous solution with keratin binding structural domain active component	?20.0	?5.0	?1.0	?0.5	?10.0
						Cremophor?PS?20	?5.00	?1.00	?1.00	?7.00	?5.00
Antiseptic	?0.10	?0.10	?0.10	?0.10	?0.10
						Rewopal?LA?3	?2.00	?1.00	?0.50	?2.00	?2.00
Citric acid	?0.20	?0.20	?0.20	?0.20	?0.20
						Stepan?PEG-600?DS	?3.00	?2.00	?2.00	?3.00	?2.50
Water	Add to 100	Add to 100	Add to 100	Add to 100	Add to 100

The oil-in-water type whipped cream

?	Emulsion 1	?	Emulsion 2	?
					?	Weight %	Volume %	Weight %	Volume %
Stearic acid	5.00	?	1.00	?

Spermol	5.50	?	?	?
					Cetearyl alcohol	?	?	2.00	?
The PEG-40 stearate	8.50	?	?	?
					The PEG-20 stearate	?	?	1.00	?
Caprylic/capric triglyceride	4.00	?	2.00	?
					The C12-15 alkyl benzoate	10.00	?	15.00	?
Cyclomethicone	4.00	?	?	?
					Dimethicone	?	?	0.50	?
Aqueous solution with keratin binding structural domain active component	5.0	?	10.0	?
					The isostearic acid monooctyl ester	?	?	5.00	?
The myristyl myristinate	?	?	2.00	?
					Ceresine	1.50	?	?	?
Glycerol	?	?	3.00	?
					Lightscreening agent	?	?	?	?
Hydroxypropyl starch phosphate	1.00	?	3.50	?
					BHT	?	?	0.02	?
Sodium ethylene diamine tetracetate	0.50	?	0.10	?
					Aromatic, antiseptic	In right amount	?	In right amount	?
Coloring agent	In right amount	?	In right amount	?
					Potassium hydroxide	In right amount	?	In right amount	?
Water	Add to 100	?	Add to 100	?
					?	PH transfers to 6.5-7.5	?	PH transfers to 5.0-6.0	?
Emulsion 1	?	70	?	?
					Emulsion 2	?	?	?	35
Gas (nitrogen)	?	30	?	?
					Gas (helium)	?	?	?	65

Conditioner shampoo with Margarita powder

?	1	?2	?3
				Polyquaternary ammonium salt-10	0.50	?0.50	?0.40
Lauryl alcohol sodium sulfate	9.00	?8.50	?8.90
				Cocoamidopropyl	2.50	?2.60	?3.00
Benzophenone-4	1.50	?0.50	?1.00
				Aqueous solution with keratin binding structural domain active component	1.0	?5.0	?0.5
Margarita powder aqueous solution with keratin binding structural domain active component	2.00	?2.50	?
				Sodium ethylene diamine tetracetate	0.10	?0.15	?0.05
Antiseptic, aromatic, thickening agent	In right amount	In right amount	In right amount
				Water	Add to 100	Add to 100	Add to 100

Regulate pH to 6.0

The cleaning conditioner shampoo

?	1	?2	?3
				Polyquaternary ammonium salt-10	0.50	?0.50	?0.50
Lauryl alcohol sodium sulfate	9.00	?8.50	?9.50
				Aqueous solution with keratin binding structural domain active component	5.0	?0.1	?3.0
Benzophenone-3	1.00	?1.50	?0.50
				The imido sodium succinate	0.20	?0.20	?0.80
Antiseptic, aromatic, thickening agent	In right amount	In right amount	In right amount

Water

Add to 100

Regulate pH to 6.0

Cleaning conditioner shampoo with volume effects

?	1	?2	?3
				Lauryl alcohol sodium sulfate	10.00	?10.50	?11.00
The ethylhexyl Methoxycinnamate	2.00	?1.50	?2.30
				Aqueous solution with keratin binding structural domain active component	10.0	?0.1	?0.5
Cocoamidopropyl	2.50	?2.60	?2.20
				Sodium ethylene diamine tetracetate	0.01	?0.10	?0.01
Antiseptic, aromatic oil, thickening agent	In right amount	In right amount	In right amount
				Water	Add to 100	Add to 100	Add to 100

Regulate pH to 6.0

The gel cream

?	1	?2	?3	?4
					Acrylate/C10-30 alkyl acrylate cross-linked polymer	0.40	?0.35	?0.40	?0.35
Polyacrylic acid	0.20	?0.22	?0.20	?0.22
					Xanthan gum	0.10	?0.13	?0.10	?0.13
Cetearyl alcohol	3.00	?2.50	?3.00	?2.50
					The C12-15 alkyl benzoate	4.00	?4.50	?4.00	?4.50
Caprylic/capric triglyceride	3.00	?3.50	?3.00	?3.50
					Uvinul?A?Plus ^TM	2.00	?1.50	?0.75	?1.00
UvaSorb?K2A	?	?3.00	?	?
					The ethylhexyl Methoxycinnamate	3.00	?	?1.00	?

Two ethylhexyl phenol anisyl triazines	?	1.50	?	?2.00
					Butyl methoxydibenzoylmethise	?	?	?2.00	?
Phenyl bisbenzimidazole tetrasulfonic acid disodium	2.50	?	?0.50	?2.00
					The ethylhexyl triazinone	4.00	?	?3.00	?4.00
1-Octyl acrylate	?	4.00	?	?
					Diethylhexyl fourth ammonia triazinone	1.00	?	?	?2.00
Phenylbenzimidazolesulfonic acid	0.50	?	?3.00	?
					Di-2-ethylhexylphosphine oxide-benzotriazole base tetramethyl butylbenzene phenol	2.00	?	?0.50	?1.50
Ethylhexyl salicylate	?	?	?3.00	?
					The drometrizole trisiloxanes	?	?	?0.50	?
The Terephthalidene Dicamphor Sulfonic Acid	?	1.50	?	?1.00
					Diethylhexyl 2,6-naphthylene ester	3.50	4.00	?7.00	?9.00
The meticulous powder of titanium dioxide	1.00	?	?3.00	?
					The meticulous powder of zinc oxide	?	?	?	?0.25
Aqueous solution with keratin binding structural domain active component	0.1	0.5	?1.0	?0.02
					The ring dimethyl polysiloxane	5.00	5.50	?5.00	?5.50
The dimethicone polydimethylsiloxane	1.00	0.60	?1.00	?0.60
					Glycerol	1.00	1.20	?1.00	?1.20
Sodium hydroxide	In right amount	In right amount	In right amount	In right amount
					Antiseptic	0.30	0.23	?0.30	?0.23
Aromatic	0.20	?	?0.20	?
					Water	Add to 100	Add to 100	Add to 100	Add to 100
Regulate pH to 6.0	?	?	?	?

The oil-in-water type sunscreen formulations

?

1

2

3

?4

?5

?6

?7

Glyceryl monostearate SE

0.50

1.00

3.00

?

?1.50

?

Tristerin

2.00

?

1.00

?2.00

?4.00

?

Stearic acid

?

3.00

?

?2.00

?

The PEG-40 stearate

0.50

?

?2.00

?

Hexadecanyl phosphate

?

?1.00

?

Cetearyl alcohol sodium sulfate

?

?0.75

Octadecanol

?

3.00

?

?2.00

?0.60

Spermol

2.50

1.10

?

?1.50

?0.60

?

?2.00

Aqueous solution with keratin binding structural domain active component

10.0

0.5

3.0

?5.0

?0.1

?0.02

?7.5

Uvinul?A?Plus ^TM

2.00

1.50

0.75

?1.00

?2.10

?4.50

?5.00

UVASorb?K2A

?

The ethylhexyl methoxy cinnamic acid

?

?5.00

?6.00

?8.00

Two ethylhexyl phenol anisyl triazines

?

1.50

?

?2.00

?2.50

?

?2.50

Butyl methoxy dibenzoyl methane

?

2.00

?

?2.00

?1.50

?

Phenyl bisbenzimidazole tetrasulfonic acid disodium

2.50

?

0.50

?2.00

?

?0.30

?

The ethylhexyl triazinone

4.00

?

3.00

?4.00

?

?2.00

?

1-Octyl acrylate

?

4.00

?

?7.50

Diethylhexyl fourth ammonia triazinone

1.00

?

?2.00

?1.00

?

?1.00

Phenylbenzimidazolesulfonic acid

0.50

?

3.00

?

Di-2-ethylhexylphosphine oxide-benzotriazole base tetramethyl butylbenzene phenol

2.00

?

0.50

?1.50

?2.50

?

Ethylhexyl salicylate

?

3.00

?

?5.00

The drometrizole trisiloxanes

?

0.50

?

?1.00

?

The Terephthalidene Dicamphor Sulfonic Acid

?

1.50

?

?1.00

?

?0.50

Diethylhexyl 2,6-naphthylene ester

3.50

?

?7.00

?

?6.00

?9.00

?

The meticulous powder of titanium dioxide

1.00

?

?3.00

?

?3.50

?

?1.50

The meticulous powder of zinc oxide

?

?0.25

?

?2.00

?

The C12-15 alkyl benzoate

?

0.25

?

?4.00

?7.00

?

Two caprylyl ethers

?

?3.50

?

?2.00

?

Butanediol dicaprylate/dicaprate

5.00

?

?6.00

?

Cocos nucifera oil glyceride

?

?6.00

?

?2.00

?

Dimethicone

0.50

?

?1.00

?

?2.00

?

Cyclomethicone

2.00

?

?0.50

?

?0.50

?

Adeps Bovis seu Bubali resin

?

2.00

?

PVP hexadecene copolymer

0.20

?

?0.50

?

?1.00

?

Glycerol

3.00

7.50

?

?7.50

?5.00

?

?2.50

Xanthan gum

0.15

?

?0.05

?

?0.30

?

Carbomer sodium

?

0.20

?

?0.15

?0.25

?

Vitamin e acetate

0.60

?

?0.23

?

?0.70

?1.00

?

Fucogel?1000

?

3.00

?10.0?0

?

Glycine Semen Glycines (Semen sojae atricolor) oil

?

?0.50

?

?1.50

?1.00

The ethyl hexyl oxy glycine

0.30

?

DMDM?Hydantaufin

?

0.60

?0.40

?0.20

?

Glyacil-L

?

?0.18

?0.20

?

Methyl parahydroxybenzoate

0.15

?

?0.25

?

?0.50

?

Phenoxyethanol

1.00

0.40

?

?0.40

?0.50

?0.40

Sodium versenate

0.02

?

?0.05

?

The imido succinic acid

?

?0.25

?1.00

?

Ethanol

2.00

1.50

?

?3.00

?

?1.20

?5.00

Aromatic

0.10

0.25

?0.30

?

?0.40

?0.20

?

Water

Add to 100

Aqueous dispersion

?	1	2	3	?4	?5
						Ceteareth	1.00	?	?	?0.50	?
Spermol	?	?	1.00	?	?
						Carbomer sodium	?	0.20	?	?0.30	?
Acrylate/C10-30 alkyl acrylate cross-linked polymer	0.50	?	0.40	?0.10	?0.50
						Xanthan gum	?	0.30	0.15	?	?
Aqueous solution with keratin binding structural domain active component	5.0	0.5	3.0	?0.1	?10.0
						Uvinul?A?Plus ^TM	2.00	1.50	0.75	?1.00	?2.10
UVASorb?K2A	?	3.50	?	?	?
						The ethylhexyl methoxy cinnamic acid	?	?	?	?	?5.00
Two ethylhexyl phenol anisyl triazines	?	1.50	?	?2.00	?2.50
						Butyl methoxy dibenzoyl methane	?	?	2.00	?	?2.00
Phenyl bisbenzimidazole tetrasulfonic acid disodium	2.50	?	0.50	?2.00	?
						The ethylhexyl triazinone	4.00	?	3.00	?4.00	?
1-Octyl acrylate	?	4.00	?	?	?
						Diethylhexyl fourth ammonia triazinone	1.00	?	?	?2.00	?1.00
Phenylbenzimidazolesulfonic acid	0.50	?	3.00	?	?
						Di-2-ethylhexylphosphine oxide-benzotriazole base tetramethyl butylbenzene phenol	2.00	?	0.50	?1.50	?2.50
Ethylhexyl salicylate	?	?	3.00	?	?
						The drometrizole trisiloxanes	?	?	0.50	?	?

The Terephthalidene Dicamphor Sulfonic Acid	?	?1.50	?	?1.00	1.00
						Diethylhexyl 2,6-naphthylene ester	?	?	?7.00	?	9.00
The meticulous powder of titanium dioxide	1.00	?	?3.00	?	3.50
						The meticulous powder of zinc oxide	?	?	?	?0.25	?
The C12-15 alkyl benzoate	2.00	?2.50	?	?	?
						Two caprylyl ethers	?	?4.00	?	?	?
Butanediol dicaprylate/dicaprate	4.00	?	?2.00	?6.00	?
						Two caprylyl carbonic esters	?	?2.00	?6.00	?	?
Dimethicone	?	?0.50	?1.00	?	?
						Phenyl trimethyl silicone oil	2.00	?	?0.50	?	?
Adeps Bovis seu Bubali resin	?	?2.00	?	?5.00	?
						PVP hexadecene copolymer	0.50	?	?	?0.50	1.00
Herba leucadis ciliatae alkyl PVP (Tricontanyl PVP)	0.50	?	?1.00	?	?
						Ethylhexyl glycerol	?	?	?1.00	?	0.80
Glycerol	3.00	?7.50	?	?7.50	8.50
						The Semen Glycines glycine	?	?	?1.50	?	1.00
Vitamin e acetate	0.50	?	?0.25	?	1.00
						α glucose rutin (Glucosilrutin)	0.60	?	?	?0.25	?
Fucogel?1060	?	?2.50	?0.50	?	2.00
						DMDM?Hydanaufin	?	?0.60	?0.45	?0.25	?
Glyacil-S	0.20	?	?	?	?
						Methyl parahydroxybenzoate	0.50	?	?0.25	?0.15	?
Phenoxyethanol	0.50	?0.40	?	?1.00	?
						Sodium versenate	?	?0.01	?0.05	?	0.10
Ethanol	3.00	?2.00	?1.50	?	7.00
						Aromatic	0.20	?	?0.05	?0.40	?
Water	To 100	To 100	To 100	To 100	To 100

The oil-in-water type sunscreen emulsion

?	1	2	3	?4	?5
						Cetyl dimethicone copolyol	?	2.50	?	?4.00	?
Polyglyceryl-2 dimerization hydroxy stearic acid ester	5.00	?	?	?	?4.50
						PEG-30 dimerization hydroxy stearic acid ester	?	?	5.00	?	?
Aqueous solution with keratin binding structural domain active component	5.0	1.0	10.0	?0.5	?0.1
						Uvinul?A?Plus ^TM	2.00	1.50	0.75	?1.00	?2.10
UVASorb?K2A	?	2.00	?	?	?
						The ethylhexyl Methoxycinnamate	?	?	?	?	?5.00
Diethylhexyl phenol anisyl triazine	?	1.50	?	?2.00	?2.50
						Butyl methoxy dibenzoyl methane	?	?	2.00	?	?2.00
Phenyl bisbenzimidazole tetrasulfonic acid disodium	2.50	?	0.50	?2.00	?
						The ethylhexyl triazinone	4.00	?	3.00	?4.00	?
1-Octyl acrylate	?	4.00	?	?	?
						Two ethylhexyl fourth ammonia triazinones	1.00	?	?	?2.00	?1.00
Phenylbenzimidazolesulfonic acid	0.50	?	3.00	?	?
						Di-2-ethylhexylphosphine oxide-benzotriazole base tetramethyl butylbenzene phenol	2.00	?	0.50	?1.50	?2.50
Ethylhexyl salicylate	?	?	3.00	?	?
						The drometrizole trisiloxanes	?	?	0.50	?	?
The Terephthalidene Dicamphor Sulfonic Acid	?	1.50	?	?1.00	?1.00
						Diethylhexyl 2,6-naphthylene ester	?	?	7.00	?	?4.00
The meticulous powder of titanium dioxide	1.00	?	3.00	?	?3.50
						The meticulous powder of zinc oxide	?	?	?	?0.25	?
Mineral oil	?	12.00	10.00	?	?8.00

The C12-15 alkyl benzoate	?	?	?	?9.00	?
						Two caprylyl ethers	10.00	?	?	?	?7.00
Butanediol dicaprylate/dicaprate	?	?	?2.00	?8.00	?4.00
						Two caprylyl carbonic esters	5.00	?	?6.00	?	?
Dimethicone	?	?4.00	?1.00	?5.00	?
						Cyclomethicone	2.00	?25.00	?	?	?2.00
Adeps Bovis seu Bubali resin	?	?	?3.00	?	?
						Vaseline	?	?4.50	?	?	?
PVP hexadecene copolymer	0.50	?	?	?0.50	?1.00
						Ethylhexyl glycerol	?	?0.30	?1.00	?	?0,50
Glycerol	3.00	?7.50	?	?7.50	?8.50
						The Semen Glycines glycine	?	?1.00	?1.50	?	?1.00
MgSO ₄	1.00	?0.50	?	?0.50	?
						MgCl ₂	?	?	?1.00	?	?0.70
Vitamin e acetate	0.50	?	?0.25	?	?1.00
						Ascorbyl palmitate	0.50	?	?	?2.00	?
Fucogel?1000	?	?	?	?3.50	?1.00
						DMDM?Hydanaufin	?	?0.60	?0.40	?0.20	?
Methyl parahydroxybenzoate	0.50	?	?0.25	?0.15	?
						Phenoxyethanol	0.50	?0.40	?	?1.00	?
Sodium versenate	0.12	?0.05	?	?0.30	?
						Ethanol	3.00	?	?1.50	?	?5.00
Aromatic	0.20	?	?0.40	?0.35	?
						Water	Add to 100	Add to 100	Add to 100	Add to 100	Add to 100

Stick

?	1	2	?3	?4
					Caprylic/capric triglyceride	12.00	10.00	?6.00	?
Octyl dodecanol	7.00	14.00	?8.00	?3.00
					Butanediol dicaprylate/dicaprate	?	?	?	?12.00
Tetramethylolmethane base tetraoctyl stearate	10.00	6.00	?8.00	?7.00
					Polyglyceryl-3 diisopstearate	2.50	?	?	?
Two-polyamides base adipic acid two sweet esters-2	9.00	8.00	?10.00	?8.00
					Cetearyl alcohol	8.00	11.00	?9.00	?7.00
The myristyl myristinate	3.50	3.00	?4.00	?3.00
					Cera Flava	5.00	5.00	?6.00	?6.00
Brazil wax	1.50	2.00	?2.00	?1.50
					Cera alba	0.50	0.50	?0.50	?0.40
C16-40 alkyl stearate	?	1.50	?1.50	?1.50
					Aqueous solution with keratin binding structural domain active component	0.5	3.0	?1.0	?5.0
Uvinul?A?Plus ^TM	2.00	1.50	?0.75	?9.00
					UVASorb?K2A	?	2.00	?	?4.00
The ethylhexyl methoxy cinnamic acid	?	3.00	?	?
					Two ethylhexyl phenol anisyl triazines	?	1.50	?	?2.00
Butyl methoxy dibenzoyl methane	?	?	?2.00	?
					Phenyl bisbenzimidazole tetrasulfonic acid disodium	2.50	?	?0.50	?2.00
The ethylhexyl triazinone	4.00	?	?3.00	?4.00
					1-Octyl acrylate	?	4.00	?	?
Two ethylhexyl fourth ammonia triazinones	1.00	?	?	?2.00
					Phenylbenzimidazolesulfonic acid	0.50	?	?3.00	?
Di-2-ethylhexylphosphine oxide-benzotriazole base tetramethyl butylbenzene	2.00	?	?0.50	?1.50

Phenol	?	?	?	?
					Ethylhexyl salicylate	?	?	?3.00	?
The drometrizole trisiloxanes	?	?	?0.50	?
					The Terephthalidene Dicamphor Sulfonic Acid	?	?1.50	?	?1.00
Diethylhexyl 2,6-naphthylene ester	?	?	?7.00	?
					The meticulous powder of titanium dioxide	?1.00	?	?3.00	?
The meticulous powder of zinc oxide	?	?	?	?0.25
					Vitamin e acetate	?0.50	?1.00	?	?
Ascorbyl palmitate	?0.05	?	?0.05	?
					Buxux?Chinensis	?2.00	?1.00	?	?1.00
Aromatic, BHT	?0.10	?0.25	?	?0.35
					Oleum Ricini	To 100	To 100	To 100	To 100

The PIT emulsifying agent

?

1

?2

?3

?4

?5

?6

?7

?8

Glyceryl monostearate SE

0.50

?2.00

?3.00

?5.00

?

?0.50

?4.00

?

Glyceryl isostearate

?

?3.50

?4.00

?2.00

?

Different spermaceti alcohol ether-20

?

?0.50

?

?2.00

?

Ceteareth-12

?

?5.00

?

?1.00

?

?3.50

?5.00

Ceteareth-20

?

?5.00

?

?1.00

?

?3.50

The PEG-100 stearate

?

?2.80

?

?2.30

?3.30

?

Spermol

5.20

?

?1.20

?1.00

?1.30

?

?0.50

?0.30

Cetyl palmitate

2.50

?1.20

?

?1.50

?

?0.50

?

?1.50

The cetyl dimethicone copolyol

?

?0.50

?

?1.00

?

Polyglyceryl-2

?

?0.75

?0.30

?

Has the keratin binding structural domain

0.1

?5.0

?0.01

?0.5

?3.0

?0.25

?10.0

?3.0

The aqueous solution of active component

?

Uvinul?A?Plus ^TM

2.00

1.50

?0.75

?1.00

?2.10

?4.50

?5.00

?2.10

UVASorb?K2A

?

?4.00

?

?1.50

?

The ethylhexyl methoxy cinnamic acid

?

?5.00

?6.00

?8.00

?5.00

Two ethylhexyl phenol anisyl triazines

?

1.50

?

?2.00

?2.50

?

?2.50

Butyl methoxybenzophenone

?

?2.00

?

?2.00

?1.50

?

?2.00

Phenyl bisbenzimidazole tetrasulfonic acid disodium

2.50

?

?0.50

?2.00

?

?0.30

?

The ethylhexyl triazinone

4.00

?

?3.00

?4.00

?

?2.00

?

1-Octyl acrylate

?

4.00

?

?7.50

?

Two ethylhexyl fourth ammonia triazinones

1.00

?

?2.00

?1.00

?

?1.00

Phenylbenzimidazolesulfonic acid

0.50

?

?3.00

?

2.00

?

?0.50

?1.50

?2.50

?

?2.50

Ethylhexyl salicylate

?

?3.00

?

?5.00

?

The drometrizole trisiloxanes

?

?0.50

?

?1.00

?

The Terephthalidene Dicamphor Sulfonic Acid

?

1.50

?

?1.00

?

?0.50

?1.00

Diethylhexyl 2,6-naphthylene ester

?

?7.00

?

?10.0?0

?7.50

?

?8.00

The meticulous powder of titanium dioxide

1.00

?

?3.00

?

?3.50

?

?1.50

?3.50

The meticulous powder of zinc oxide

?

?0.25

?

?2.00

?

The C12-15 alkyl benzoate

3.50

?

?6.35

?

?0.10

Cocos nucifera oil glyceride

?

3.00

?

?3.00

?

?1.00

Two caprylyl ethers

4.50

?

Two caprylyl carbonic esters

?

4.30

?

?3.00

?

?7.00

Dibutyl adipate

?

?0.50

?

?0.30

Phenyl trimethyl silicone oil

2.00

?

?3.50

?

?2.00

?

Cyclomethicone

?

3.00

?

The ethyl galactomannan

?

0.50

?

?2.00

?

Hydrogenation cocos nucifera oil glyceride

?

?3.00

?4.00

?

Spermaceti (Abil Wax) 2440

?

?1.50

?2.00

?

PVP hexadecene copolymer

?

?1.00

?1.20

?

Glycerol

4.00

6.00

?5.00

?

?8.00

?10.0?0

?

Vitamin e acetate

0.20

0.30

?0.40

?

?0.30

?

Adeps Bovis seu Bubali resin

?

2.00

?

?3.60

?

?2.00

?

Iodine third butyl carbamate

0.12

?

?0.20

?

Fucogel?1000

?

?0.10

?

DMDM?Hydanaufin

0.10

?

?0.12

?

?0.13

?

Methyl parahydroxybenzoate

?

0.50

?0.30

?

?0.35

?

Phenoxyethanol

0.50

0.40

?

?1.00

?

Ocaufxy glycerol

?

0.30

?

?1.00

?

?0.35

?

Ethanol

2.00

?

?2.00

?

?5.00

?

Sodium versenate

0.40

?

?0.15

?

?0.20

?

Aromatic

0.20

?

?0.20

?

?0.24

?0.16

?0.10

Water

Add to 100

The gel cream

?	1	?2	?3	?4
					Acrylate/C10-30 alkyl acrylate is handed over	0.40	?0.35	?0.40	?0.35

Linked polymer	?	?	?	?
					Polyacrylic acid	0.20	?0.22	?0.20	?0.22
Luvigel?EM	1.50	?2.50	?2.80	?3.50
					Xanthan gum	0.10	?0.13	?0.10	?0.13
Cetearyl alcohol	3.00	?2.50	?3.00	?2.50
					The C12-15 alkyl benzoate	4.00	?4.50	?4.00	?4.50
Caprylic/capric triglyceride	3.00	?3.50	?3.00	?3.50
					The meticulous powder of titanium dioxide	1.00	?	?1.50	?
The meticulous powder of zinc oxide	?	?2.00	?	?0.25
					Aqueous solution with keratin binding structural domain active component	0.5	?10.0	?3.0	?5.0
Dihydroxyaceaufn	?	?	?3.00	?5.00
					The ring dimethyl polysiloxane	5.00	?5.50	?5.00	?5.50
The dimethicone dimethione	1.00	?0.60	?1.00	?0.60
					Glycerol	1.00	?1.20	?1.00	?1.20
Sodium hydroxide	In right amount	In right amount	In right amount	In right amount
					Antiseptic	0.30	?0.23	?0.30	?0.23
Aromatic	0.20	?	?0.20	?
					Water	Add to 100	Add to 100	Add to 100	Add to 100
Regulate pH to 6.0	?	?	?	?

The self-service U.S. black preparation of oil-in-water type

?

1

2

?3

?4

?5

?6

?7

Glyceryl monostearate SE

0.50

1.00

?3.00

?

?1.50

?

Tristerin

2.00

?

?1.00

?2.00

?4.00

?

Stearic acid

?

3.00

?

?2.00

?

The PEG-40 stearate

0.50

?

?2.00

?

Hexadecanyl phosphate

?

?1.00

?

Cetearyl alcohol sulfate

?

?0.75

Octadecanol

?

?3.00

?

?2.00

?0.60

Spermol

2.50

?1.10

?

?1.50

?0.60

?

?2.00

Aqueous solution with keratin binding structural domain active component

0.1

?0.5

?0.025

?5.0

?3.0

?10.0

?1.0

Dihydroxy acetone (Dihydroxyaceaufn)

?

?3.00

?5.00

?

?4

?

The meticulous powder of titanium dioxide

1.00

?

?1.50

?

?1.50

The meticulous powder of zinc oxide

?

?0.25

?

?2.00

?

The C12-15 alkyl benzoate

?

?0.25

?

?4.00

?7.00

?

Two caprylyl ethers

?

?3.50

?

?2.00

?

Butanediol dicaprylate/dicaprate

5.00

?

?6.00

?

Cocos nucifera oil glyceride

?

?6.00

?

?2.00

?

Dimethicone

0.50

?

?1.00

?

?2.00

?

Cyclomethicone

2.00

?

?0.50

?

?0.50

?

Adeps Bovis seu Bubali resin

?

?2.00

?

PVP hexadecene copolymer

0.20

?

?0.50

?

?1.00

?

Glycerol

3.00

?7.50

?

?7.50

?5.00

?

?2.50

Xanthan gum

0.15

?

?0.05

?

?0.30

?

Carbomer sodium

?

?0.20

?

?0.15

?0.25

?

Vitamin e acetate

0.60

?

?0.23

?

?0.70

?1.00

?

Fucogel?1000

?

?3.00

?10.00

?

The Semen Glycines glycine

?

?0.50

?

?1.50

?1.00

The ethyl hexyl oxy glycine

0.30

?

DMDM?Hydanaufin

?

?0.60

?0.40

?0.20

?

Glyacil-L

?

0.18

?0.20

?

Methyl parahydroxybenzoate

0.15

?

0.25

?

?0.50

?

Phenoxyethanol

1.00

0.40

?

?0.40

?0.50

?0.40

Sodium versenate

0.02

?

0.05

?

The imido succinic acid

?

0.25

?1.00

?

Ethanol

2.00

1.50

?

3.00

?

?1.20

?5.00

Aromatic

0.10

0.25

0.30

?

?0.40

?0.20

?

Water

Add to 100

The oil-in-water type cosmetics

?

1

2

?3

?4

?5

?6

?7

Glyceryl monostearate SE

0.50

1.00

?3.00

?

?1.50

?

Tristerin

2.00

?

?1.00

?2.00

?4.00

?

Stearic acid

?

3.00

?

?2.00

?

The PEG-40 stearate

0.50

?

?2.00

?

Hexadecanyl phosphate

?

?1.00

?

Cetearyl alcohol sulfate

?

?0.75

Octadecanol

?

?3.00

?

?2.00

?0.60

Spermol

2.50

1.10

?

?1.50

?0.60

?

?2.00

Aqueous solution with keratin binding structural domain active component

3.0

5.0

?2.0

?0.5

?1.0

?5.0

?10.0

Titanium dioxide

10.00

12.00

?9.00

?8.50

?11.00

?9.50

?10.00

Ferrum oxide

2.00

4.00

?3.00

?5.00

?3.40

?6.00

?4.40

Zinc oxide

?

4.00

?

?2.00

?

?3.00

?

The C12-15 alkyl benzoate

?

0.25

?

?4.00

?7.00

?

Dicaprylyl ether

?

?3.50

?

?2.00

?

Butanediol dicaprylate/dicaprate

5.00

?

?6.00

?

Cocos nucifera oil glyceride

?

?6.00

?

?2.00

?

Dimethicone

0.50

?

?1.00

?

?2.00

?

Cyclomethicone

2.00

?

?0.50

?

?0.50

?

Adeps Bovis seu Bubali resin

?

?2.00

?

PVP hexadecene copolymer

0.20

?

?0.50

?

?1.00

?

Glycerol

3.00

?7.50

?

?7.50

?5.00

?

?2.50

Xanthan gum

0.15

?

?0.05

?

?0.30

?

Carbomer sodium

?

?0.20

?

?0.15

?0.25

?

Vitamin e acetate

0.60

?

?0.23

?

?0.70

?1.00

?

The Semen Glycines glycine

?

?0.50

?

?1.50

?1.00

The ethyl hexyl oxy glycine

0.30

?

DMDM?Hydanaufin

?

?0.60

?0.40

?0.20

?

Glyacil-L

?

?0.18

?0.20

?

Methyl parahydroxybenzoate

0.15

?

?0.25

?

?0.50

?

Phenoxyethanol

1.00

?0.40

?

?0.40

?0.50

?0.40

Sodium versenate

0.02

?

?0.05

?

The imido succinic acid

?

?0.25

?1.00

?

Ethanol

2.00

?1.50

?

?3.00

?

?1.20

?5.00

Aromatic

0.10

?0.25

?0.30

?

?0.40

?0.20

?

Water

Add to 100

Self-service U.S. Heisui River dispersion

?	?1	?2	?3	?4	?5
						Ceteareth-20	?1.00	?	?	?0.50	?
Spermol	?	?	1.00	?	?

Luvigel?EM	?	2.00	?	?2.50	?2.00
						Acrylate/C10-30 alkyl acrylate cross-linked polymer	0.50	?	?0.40	?0.10	?0.50
Xanthan gum	?	0.30	?0.15	?	?
						Aqueous solution with keratin binding structural domain active component	3.0	1.0	?0.5	?0.1	?5.0
Dihydroxy acetone (Dihydroxyaceaufn)	?	?	?3.00	?5.00	?
						Uvinul?A?Plus ^TM	2.00	1.50	?0.75	?1.00	?2.10
The meticulous powder of titanium dioxide	1.00	?	?1.00	?	?1.00
						The meticulous powder of zinc oxide	?	1.90	?	?0.25	?
The C12-15 alkyl benzoate	2.00	2.50	?	?	?
						Dicaprylyl ether	?	4.00	?	?	?
Butanediol dicaprylate/dicaprate	4.00	?	?2.00	?6.00	?
						The dioctyl carbonic ester	?	2.00	?6.00	?	?
Dimethicone	?	0.50	?1.00	?	?
						Phenyl trimethyl silicone oil	2.00	?	?0.50	?	?
Adeps Bovis seu Bubali resin	?	2.00	?	?5.00	?
						PVP hexadecene copolymer	0.50	?	?	?0.50	?1.00
Herba leucadis ciliatae alkyl PVP	0.50	?	?1.00	?	?
						Ethylhexyl glycerol	?	?	?1.00	?	?0.80
Glycerol	3.00	7.50	?	?7.50	?8.50
						The Semen Glycines glycine	?	?	?1.50	?	?1.00
Vitamin e acetate	0.50	?	?0.25	?	?1.00
						The phlorose rutin	0.60	?	?	?0.25	?
DMDM?Hydan?aufin	?	0.60	?0.45	?0.25	?
						Glyacil-S	0.20	?	?	?	?
Methyl parahydroxybenzoate	0.50	?	?0.25	?0.15	?

Phenoxyethanol	0.50	0.40	?	1.00	?
						Sodium versenate	?	0.01	?0.05	?	?0.10
Ethanol	3.00	2.00	?1.50	?	?7.00
						Aromatic	0.20	?	?0.05	0.40	?
Water	Add to 100	Add to 100	Add to 100	Add to 100	Add to 100

Aqueous dispersion after the Exposure to Sunlight

?	?1	?2	?3	?4	?5
						Ceteareth-20	?1.00	?	?	?0.50	?
Spermol	?	?	?1.00	?	?
						Luvigel EM	?	?2.00	?	?2.50	?2.00
Acrylate/C10-30 alkyl acrylate cross-linked polymer	?0.50	?0.30	?0.40	?0.10	?0.50
						Xanthan gum	?	?0.30	?0.15	?	?
Aqueous solution with keratin binding structural domain active component	?0.1	?5.0	?0.5	?3.0	?1.0
						The C12-15 alkyl benzoate	?2.00	?2.50	?	?	?
Dicaprylyl ether	?	?4.00	?	?	?
						Butanediol dicaprylate/dicaprate	?4.00	?	?2.00	?6.00	?
The dioctyl carbonic ester	?	?2.00	?6.00	?	?
						Dimethicone	?	?0.50	?1.00	?	?
Phenyl trimethyl silicone oil	?2.00	?	?0.50	?	?
						Herba leucadis ciliatae alkyl PVP	?0.50	?	?1.00	?	?
Ethylhexyl glycerol	?	?	?1.00	?	?0.80
						Glycerol	?3.00	?7.50	?	?7.50	?8.50
The Semen Glycines glycine	?	?	?1.50	?	?1.00

Vitamin e acetate	0.50	?	?0.25	?	?1.00
						Phlorose rutin (Alpha-Glucosilrutin)	0.60	?	?	?0.25	?
Sodium versenate	?	0.01	?0.05	?	?0.10
						Ethanol	15.00	10.00	?8.00	?12.00	?9.00
Aromatic	0.20	?	?0.05	?0.40	?
						Water	Add to 100	Add to 100	Add to 100	Add to 100	Add to 100

Water-in-oil emulsion

?	1	?2	?3	?4	?5
						The cetyl dimethicone copolyol	?	?2.50	?	?4.00	?
Polyglyceryl-2 dimerization hydroxy stearic acid ester	5.00	?	?	?	?4.50
						PEG-30 dimerization hydroxy stearic acid ester	?	?	?5,00	?	?
Aqueous solution with keratin binding structural domain active component	5.0	?10.0	?0.1	?0.5	?1.0
						Uvinul?A?Plus ^TM	2.00	?1.50	?0.75	?1.00	?2.10
The meticulous powder of titanium dioxide	1.00	?	?3.00	?	?3.50
						The meticulous powder of zinc oxide	?	?0.90	?	?0.25	?
Mineral oil	?	?12.00	?10.00	?	?8.00
						The C12-15 alkyl benzoate	?	?	?	?9.00	?
Two caprylyl ethers	10.00	?	?	?	?7.00
						Butanediol dicaprylate/dicaprate	?	?	?2.00	?8.00	?4.00
Two caprylyl carbonic esters	5.00	?	?6.00	?	?
						Dimethicone	?	?4.00	?1.00	?5.00	?
Cyclomethicone	2.00	?25.00	?	?	?2.00
						Adeps Bovis seu Bubali resin	?	?	?3.00	?	?

Vaseline	?	4.50	?	?	?
						PVP hexadecene copolymer	0.50	?	?	?0.50	?1.00
Ethylhexyl glycerol	?	0.30	1.00	?	?0.50
						Glycerol	3.00	7.50	?	?7.50	?8.50
The Semen Glycines glycine	?	1.00	1.50	?	?1.00
						MgSO ₄	1.00	0.50	?	?0.50	?
MgCl ₂	?	?	1.00	?	?0.70
						Vitamin e acetate	0.50	?	0.25	?	?1.00
Ascorbyl palmitate	0.50	?	?	?2.00	?
						Fucogel?1000	?	?	?	?3.50	?7.00
DMDM?Hydanaufin	?	0.60	0.40	?0.20	?
						Methyl parahydroxybenzoate	0.50	?	0.25	?0.15	?
Phenoxyethanol	0.50	0.40	?	?1.00	?
						Sodium versenate	0.12	0.05	?	?0.30	?
Ethanol	3.00	?	1.50	?	?5.00
						Aromatic	0.20	?	0.40	?0.35	?
Water	Add to 100	Add to 100	Add to 100	Add to 100	Add to 100

Pickering Emulsion (Pickering Emulsion)

?	1	?2	?3	?4	?5
						Mineral oil	?	?	?16.00	?16.00	?
Octyl dodecanol	9.00	?9.00	?5.00	?	?
						Caprylic/capric triglyceride	9.00	?9.00	?6.00	?	?
The C12-15 alkyl benzoate	?	?	?	?5.00	?8.00
						Butanediol dicaprylate/dicaprate	?	?	?	?	?8.00
Two caprylyl ethers	9.00	?	?	?4.00	?

Two caprylyl carbonic esters	?	?9.00	?	?	?
						Hydroxyl octacosanol hydroxy stearic acid ester	?2.00	?2.00	?2.20	?2.50	?1.50
Disteardimonium?Hecaufrite	?1.00	?0.75	?	?0.50	?0.25
						Microwax+paraffin oil	?	?0.35	?	?	?5.00
Hydroxypropyl emthylcellulose	?	?	?0.10	?	?0.05
						Dimethicone	?	?	?	?	?3.00
Aqueous solution with keratin binding structural domain active component	1.0	?0.5	?0.1	?3.0	?5.0
						Titanium dioxide+Alumina+dimethicone+water	?	?3.00	?	?	?
Titanium dioxide+trimethoxy decoyl silane	?	?2.00	?4.00	?2.00	?4.00
						Dimethyl silane silicon (Silica dimethyl silylate)	2.50	?	?	?6.00	?2.50
Boron nitride	?	?	?1.00	?	?
						Starch/Polymeric sodium metaphosphate. polymer	2.00	?	?	?	?
Tapioca	?	?0.50	?	?	?
						Sodium chloride	5.00	?7.00	?8.50	?3.00	?4.50
Glycerol	?	?	?	?1.00	?
						Sodium versenate	1.00	?1.00	?1.00	?1.00	?1.00
Vitamin e acetate	5.00	?10.00	?3.00	?6.00	?10.00
						Ascorbyl palmitate	1.00	?1.00	?	?1.00	?
Methyl parahydroxybenzoate	?	?0.60	?	?	?0.20
						Propyl p-hydroxybenzoate	?	?	?	?	?0.20
Phenoxyethanol	?	?	?0.20	?	?
						Amidine is decided dihydroxy ethyl sulfonic acid	?	?	?0.40	?0.50	?0.40
Two imidazolidinyl urea	?	?	?	?	?0.08
						Ethanol	?	?	?0.23	?0.20	?
Aromatic	5.00	?	?3.00	?4.00	?

Water	0.20	?	0.30	0.10	?
						?	Add to 100	Add to 100	Add to 100	Add to 100	Add to 100

Stick

?	?1	?2	?3	?4
					Caprylic/capric triglyceride	?12.00	?10.00	?6.00	?
Octyl dodecanol	?7.00	?14.00	?8.00	?3.00
					Butanediol dicaprylate/dicaprate	?	?	?	?12.00
Tetramethylolmethane base tetraoctyl stearate	?10.00	?6.00	?8.00	?7.00
					Polyglyceryl-3 diisopstearate	?2.50	?	?	?
Two-polyamides base adipic acid two sweet esters-2	?9.00	?8.00	?10.00	?8.00
					Cetearyl alcohol	?8.00	?11.00	?9.00	?7.00
The myristyl myristate	?3.50	?3.00	?4.00	?3.00
					Cera Flava	?5.00	?5.00	?6.00	?6.00
Brazil wax	?1.50	?2.00	?2.00	?1.50
					Cera alba	?0.50	?0.50	?0.50	?0.40
C16-40 alkyl stearate	?	?1.50	?1.50	?1.50
					Aqueous solution with keratin binding structural domain active component	?10.0	?1.0	?3.0	?0.1
Uvinul?A?Plus ^TM	?2.00	?1.50	?0.75	?9.00
					The meticulous powder of titanium dioxide	?1.00	?	?3.00	?
The meticulous powder of zinc oxide	?	?1.00	?	?0.25
					Vitamin e acetate	?0.50	?1.00	?	?
Ascorbyl palmitate	?0.05	?	?0.05	?
					Buxux?Chinensis	?2.00	?1.00	?	?1.00
Aromatic, BHT	?0.10	?0.25	?	?0.35

Semen Ricini

Add to 100

Self-service U.S. deceives PIT Emulsion

?

1

2

?3

?4

?5

?6

?7

?8

Glyceryl monostearate SE

0.50

2.00

?3.00

?5.00

?

?0.50

?4.00

?

The glyceryl isostearate

?

?3.50

?4.00

?2.00

?

Different spermaceti alcohol ether-20

?

0.50

?

?2.00

?

Ceteareth-12

?

5.00

?

?1.00

?

?3.50

?5.00

Ceteareth-20

?

5.00

?

?1.00

?

?3.50

The PEG-100 stearate

?

?2.80

?

?2.30

?3.30

?

Spermol

5.20

?

?1.20

?1.00

?1.30

?

?0.50

?0.30

The spermol cetylate

2.50

1.20

?

?1.50

?

?0.50

?

?1.50

The cetyl dimethicone copolyol

?

?0.50

?

?1.00

?

Polyglyceryl-2

?

?0.75

?0.30

?

Aqueous solution with keratin binding structural domain active component

0.1

0.5

?0.01

?5.0

?0.5

?3.0

?0.025

?10.0

Dihydroxyaceaufn

?

?3.00

?5.00

?

?4.00

?

Uvinul?A?Plus ^TM

2.00

1.50

?0.75

?1.00

?2.10

?4.50

?5.00

?2.10

The meticulous powder of titanium dioxide

1.00

?

?1.50

?

?3.50

?

?1.50

?1.00

The meticulous powder of zinc oxide

?

1.00

?

?0.25

?

?2.00

?

?1.50

The C12-15 alkyl benzoate

3.50

?

?6.35

?

?0.10

Cocos nucifera oil glyceride

?

3.00

?

?3.00

?

?1.00

Dicaprylyl ether

4.50

?

Two caprylyl carbonic esters

?

4.30

?

?3.00

?

?7.00

Dibutyl adipate

?

?0.50

?

?0.30

Phenyl trimethyl silicone oil

2.00

?

?3.50

?

?2.00

?

Cyclomethicone

?

3.00

?

The ethyl galactomannan

?

0.50

?

?2.00

?

Hydrogenation cocos nucifera oil glyceride

?

?3.00

?4.00

?

Spermaceti (Abil Wax) 2440

?

?1.50

?2.00

?

PVP hexadecene copolymer

?

?1.00

?1.20

?

Glycerol

4.00

6.00

?5.00

?

?8.00

?10.00

?

Vitamin e acetate

0.20

0.30

?0.40

?

?0.30

?

Adeps Bovis seu Bubali resin

?

2.00

?

?3.60

?

?2.00

?

Iodine third butyl carbamate

0.12

?

?0.20

?

DMDM?Hydanaufin

0.10

?

?0.12

?

?0.13

?

Methyl parahydroxybenzoate

?

0.50

?0.30

?

?0.35

?

Phenoxyethanol

0.50

0.40

?

?1.00

?

Ocaufxyglycerin

?

0.30

?

?1.00

?

?0.35

?

Ethanol

2.00

?

?2.00

?

?5.00

?

Sodium versenate

0.40

?

?0.15

?

?0.20

?

Aromatic

0.20

?

?0.20

?

?0.24

?0.16

?0.10

Water

To 100

The oleogel agent

?	1	?2	?3	?4
					Caprylic/capric triglyceride	12.00	?10.00	?6.00	?
Octyl dodecanol	7.00	?14.00	?8.00	?3.00
					Butanediol dicaprylate/dicaprate	?	?	?	?12.00
Tetramethylolmethane base tetraoctyl stearate	10.00	?6.00	?8.00	?7.00
					Polyglyceryl-3 diisopstearate	2.50	?	?	?
Two-polyamides base adipic acid two sweet esters-2	9.00	?8.00	?10.00	?8.00

The myristyl myristinate	3.50	?3.00	?4.00	?3.00
					Benaufne-34	5.00	?5.00	?6.00	?6.00
Allyl carbonate	15.00	?20.00	?18.00	?19.50
					Aqueous solution with keratin binding structural domain active component	1.0	?0.5	?3.0	?5.0
Vitamin e acetate	0.50	?1.00	?	?
					Ascorbyl palmitate	0.05	?	?0.05	?
Buxux?Chinensis	2.00	?1.00	?	?1.00
					Aromatic, BHT	0.10	?0.25	?	?0.35
Semen Ricini	Add to 100	Add to 100	Add to 100	Add to 100

Sequence table

＜110〉BASF Aktiengesellchaft

＜120〉keratin-binding polypeptides

<130>AE20040313

<140>PF55618

<141>2005-05-24

<160>1

<170>PatentIn?version?3.1

<210>1

<211>2871

<212>PRT

＜213〉homo sapiens (Homo sapiens)

<400>1

Met?Ser?Cys?Asn?Gly?Gly?Ser?His?Pro?Arg?Ile?Asn?Thr?Leu?Gly?Arg

1 5 10 15

Met?Ile?Arg?Ala?Glu?Ser?Gly?Pro?Asp?Leu?Arg?Tyr?Glu?Val?Thr?Ser

20 25 30

Gly?Gly?Gly?Gly?Thr?Ser?Arg?Met?Tyr?Tyr?Ser?Arg?Arg?Gly?Val?Ile

35 40 45

Thr?Asp?Gln?Asn?Ser?Asp?Gly?Tyr?Cys?Gln?Thr?Gly?Thr?Met?Ser?Arg

50 55 60

His?Gln?Asn?Gln?Asn?Thr?Ile?Gln?Glu?Leu?Leu?Gln?Asn?Cys?Ser?Asp

65 70 75 80

Cys?Leu?Met?Arg?Ala?Glu?Leu?Ile?Val?Gln?Pro?Glu?Leu?Lys?Tyr?Gly

85 90 95

Asp?Gly?Ile?Gln?Leu?Thr?Arg?Ser?Arg?Glu?Leu?Asp?Glu?Cys?Phe?Ala

100 105 110

Gln?Ala?Asn?Asp?Gln?Met?Glu?Ile?Leu?Asp?Ser?Leu?Ile?Arg?Glu?Met

115 120 125

Arg?Gln?Met?Gly?Gln?Pro?Cys?Asp?Ala?Tyr?Gln?Lys?Arg?Leu?Leu?Gln

130 135 140

Leu?Gln?Glu?Gln?Met?Arg?Ala?Leu?Tyr?Lys?Ala?Ile?Ser?Val?Pro?Arg

145 150 155 160

Val?Arg?Arg?Ala?Ser?Ser?Lys?Gly?Gly?Gly?Gly?Tyr?Thr?Cys?Gln?Ser

165 170 175

Gly?Ser?Gly?Trp?Asp?Glu?Phe?Thr?Lys?His?Val?Thr?Ser?Glu?Cys?Leu

180 185 190

Gly?Trp?Met?Arg?Gln?Gln?Arg?Ala?Glu?Met?Asp?Met?Val?Ala?Trp?Gly

195 200 205

Val?Asp?Leu?Ala?Ser?Val?Glu?Gln?His?Ile?Asn?Ser?His?Arg?Gly?Ile

210 215 220

His?Asn?Ser?Ile?Gly?Asp?Tyr?Arg?Trp?Gln?Leu?Asp?LysIle?Lys?Ala

225 230 235 240

Asp?Leu?Arg?Glu?Lys?Ser?Ala?Ile?Tyr?Gln?Leu?Glu?Glu?Glu?Tyr?Glu

245 250 255

Asn?Leu?Leu?Lys?Ala?Ser?Phe?Glu?Arg?Met?Asp?His?Leu?Arg?Gln?Leu

260 265 270

Gln?Asn?Ile?Ile?Gln?Ala?Thr?Ser?Arg?Glu?Ile?Met?Trp?Ile?Asn?Asp

275 280 285

Cys?Glu?Glu?Glu?Glu?Leu?Leu?Tyr?Asp?Trp?Ser?Asp?Lys?Asn?Thr?Asn

290 295 300

Ile?Ala?Gln?Lys?Gln?Glu?Ala?Phe?Ser?Ile?Arg?Met?Ser?Gln?Leu?Glu

305 310 315 320

Val?Lys?Glu?Lys?Glu?Leu?Asn?Lys?Leu?Lys?Gln?Glu?Ser?Asp?Gln?Leu

325 330 335

Val?Leu?Asn?Gln?His?Pro?Ala?Ser?Asp?Lys?Ile?Glu?Ala?Tyr?Met?Asp

340 345 350

Thr?Leu?Gln?Thr?Gln?Trp?Ser?Trp?Ile?Leu?Gln?Ile?Thr?Lys?Cys?Ile

355 360 365

Asp?Val?His?Leu?Lys?Glu?Asn?Ala?Ala?Tyr?Phe?Gln?Phe?Phe?Glu?Glu

370 375 380

Ala?Gln?Ser?Thr?Glu?Ala?Tyr?Leu?Lys?Gly?Leu?Gln?Asp?Ser?Ile?Arg

385 390 395 400

Lys?Lys?Tyr?Pro?Cys?Asp?Lys?Asn?Met?Pro?Leu?Gln?His?Leu?Leu?Glu

405 410 415

Gln?Ile?Lys?Glu?Leu?Glu?Lys?Glu?Arg?Glu?Lys?Ile?Leu?Glu?Tyr?Lys

420 425 430

Arg?Gln?Val?Gln?Asn?Leu?Val?Asn?Lys?Ser?Lys?Lys?Ile?Val?Gln?Leu

435 440 445

Lys?Pro?Arg?Asn?Pro?Asp?Tyr?Arg?Ser?Asn?Lys?Pro?Ile?Ile?Leu?Arg

450 455 460

Ala?Leu?Cys?Asp?Tyr?Lys?Gln?Asp?Gln?LysIle?Val?Hi?s?Lys?Gly?Asp

465 470 475 480

Glu?Cys?Ile?Leu?Lys?Asp?Asn?Asn?Glu?Arg?Ser?Lys?Trp?Tyr?Val?Thr

485 490 495

Gly?Pro?Gly?Gly?Val?Asp?Met?Leu?Val?Pro?Ser?Val?Gly?Leu?Ile?Ile

500 505 510

Pro?Pro?Pro?Asn?Pro?Leu?Ala?Val?Asp?Leu?Ser?Cys?Lys?Ile?Glu?Gln

515 520 525

Tyr?Tyr?Glu?Ala?Ile?Leu?Ala?Leu?Trp?Asn?Gln?Leu?Tyr?Ile?Asn?Met

530 535 540

Lys?Ser?Leu?Val?Ser?Trp?His?Tyr?Cys?Met?Ile?Asp?Ile?Glu?Lys?Ile

545 550 555 560

Arg?Ala?Met?Thr?Ile?Ala?Lys?Leu?Lys?Thr?Met?Arg?Gln?Glu?Asp?Tyr

565 570 575

Met?Lys?Thr?Ile?Ala?Asp?Leu?Glu?Leu?His?Tyr?Gln?Glu?Phe?Ile?Arg

580 585 590

Asn?Ser?Gln?Gly?Ser?Glu?Met?Phe?Gly?Asp?Asp?Asp?Lys?Arg?Lys?Ile

595 600 605

Gln?Ser?Gln?Phe?Thr?Asp?Ala?Gln?Lys?His?Tyr?Gln?Thr?Leu?Val?Ile

610 615 620

Gln?Leu?Pro?Gly?Tyr?Pro?Gln?His?Gln?Thr?Val?Thr?Thr?Thr?Glu?Ile

625 630 635 640

Thr?His?His?Gly?Thr?Cys?Gln?Asp?Val?Asn?His?Asn?Lys?Val?Ile?Glu

645 650 655

Thr?Asn?Arg?Glu?Asn?Asp?Lys?Gln?Glu?Thr?Trp?Met?Leu?Met?Glu?Leu

660 665 670

Gln?Lys?Ile?Arg?Arg?Gln?Ile?Glu?His?Cys?Glu?Gly?Arg?Met?Thr?Leu

675 680 685

Lys?Asn?Leu?Pro?Leu?Ala?Asp?Gln?Gly?Ser?Ser?His?His?Ile?Thr?Val

690 695 700

Lys?Ile?Asn?Glu?Leu?Lys?Ser?Val?Gln?Asn?Asp?Ser?Gln?Ala?Ile?Ala

705 710 715 720

Glu?Val?Leu?Asn?Gln?Leu?Lys?Asp?Met?Leu?Ala?Asn?Phe?Arg?Gly?Ser

725 730 735

Glu?Lys?Tyr?Cys?Tyr?Leu?Gln?Asn?Glu?Val?Phe?Gly?Leu?Phe?Gln?Lys

740 745 750

Leu?Glu?Asn?Ile?Asn?Gly?Val?Thr?Asp?Gly?Tyr?Leu?Asn?Ser?Leu?Cys

755 760 765

Thr?Val?Arg?Ala?Leu?Leu?Gln?Ala?Ile?Leu?Gln?Thr?Glu?Asp?Met?Leu

770 775 780

Lys?Val?Tyr?Glu?Ala?Arg?Leu?Thr?Glu?Glu?Glu?Thr?Val?Cys?Leu?Asp

785 790 795 800

Leu?Asp?Lys?Val?Glu?Ala?Tyr?Arg?Cys?Gly?Leu?Lys?Lys?Ile?Lys?Asn

805 810 815

Asp?Leu?Asn?Leu?Lys?Lys?Ser?Leu?Leu?Ala?Thr?Met?Lys?Thr?Glu?Leu

820 825 830

Gln?Lys?Ala?Gln?Gln?Ile?His?Ser?Gln?Thr?Ser?Gln?Gln?Tyr?Pro?Leu

835 840 845

Tyr?Asp?Leu?Asp?Leu?Gly?Lys?Phe?Gly?Glu?Lys?Val?Thr?Gln?Leu?Thr

850 855 860

Asp?Arg?Trp?Gln?Arg?Ile?Asp?Lys?Gln?Ile?Asp?Phe?Arg?Leu?Trp?Asp

865 870 875 880

Leu?Glu?Lys?Gln?Ile?Lys?Gln?Leu?Arg?Asn?Tyr?Arg?Asp?Asn?Tyr?Gln

885 890 895

Ala?Phe?Cys?Lys?Trp?Leu?Tyr?Asp?Arg?Lys?Arg?Arg?Gln?Asp?Ser?Leu

900 905 910

Glu?Ser?Met?Lys?Phe?Gly?Asp?Ser?Asn?Thr?Val?Met?Arg?Phe?Leu?Asn

915 920 925

Glu?Gln?Lys?Asn?Leu?His?Ser?Glu?Ile?Ser?Gly?Lys?Arg?Asp?Lys?Ser

930 935 940

Glu?Glu?Val?Gln?Lys?Ile?Ala?Glu?Leu?Cys?Ala?Asn?Ser?Ile?Lys?Asp

945 950 955 960

Tyr?Glu?Leu?Gln?Leu?Ala?Ser?Tyr?Thr?Ser?Gly?Leu?Glu?Thr?Leu?Leu

965 970 975

Asn?Ile?Pro?Ile?Lys?Arg?Thr?Met?Ile?Gln?Ser?Pro?Ser?Gly?Val?Ile

980 985 990

Leu?Gln?Glu?Ala?Ala?Asp?Val?His Ala?Arg?Tyr?Ile?Glu Leu?Leu?Thr

995 1000 1005

Arg?Ser Gly?Asp?Tyr?Tyr?Arg Phe?Leu?Ser?Glu?Met Leu?Lys?Ser

1010 1015 1020

Leu?Glu Asp?Leu?Lys?Leu?Lys Asn?Thr?Lys?Ile?Glu Val?Leu?Glu

1025 1030 1035

Glu?Glu Leu?Arg?Leu?Ala?Arg Asp?Ala?Asn?Ser?Glu Asn?Cys?Asn

1040 1045 1050

Lys?Asn Lys?Phe?Leu?Asp?Gln Asn?Leu?Gln?Lys?Tyr Gln?Ala?Glu

1055 1060 1065

Cys?Ser Gln?Phe?Lys?Ala?Lys Leu?Ala?Ser?Leu?Glu Glu?Leu?Lys

1070 1075 1080

Arg?Gln Ala?Glu?Leu?Asp?Gly Lys?Ser?Ala?Lys?Gln Asn?Leu?Asp

1085 1090 1095

Lys?Cys Tyr?Gly?Gln?Ile?Lys Glu?Leu?Asn?Glu?Lys Ile?Thr?Arg

1100 1105 1110

Leu?Thr Tyr?Glu?Ile?Glu?Asp Glu?Lys?Arg?Arg?Arg Lys?Ser?Val

1115 1120 1125

Glu?Asp Arg?Phe?Asp?Gln?Gln Lys?Asn?Asp?Tyr?Asp Gln?Leu?Gln

1130 1135 1140

Lys?Ala Arg?Gln?Cys?Glu?Lys Glu?Asn?Leu?Gly?Trp Gln?Lys?Leu

1145 1150 1155

Glu?Ser Glu?Lys?Ala?Ile?Lys Glu?Lys?Glu?Tyr?Glu Ile?Glu?Arg

1160 1165 1170

Leu?Arg Val?Leu?Leu?Gln?Glu Glu?Gly?Thr?Arg?Lys Arg?Glu?Tyr

1175 1180 1185

Glu?Asn Glu?Leu?Ala?Lys?Val Arg?Asn?His?Tyr?Asn Glu?Glu?Met

1190 1195 1200

Ser?Asn Leu?Arg?Asn?Lys?Tyr Glu?Thr?Glu?Ile?Asn Ile?Thr?Lys

1205 1210 1215

Thr?Thr Ile?Lys?Glu?Ile?Ser Met?Gln?Lys?Glu?Asp Asp?Ser?Lys

1220 1225 1230

Asn?Leu Arg?Asn?Gln?Leu?Asp Arg?Leu?Ser?Arg?Glu Asn?Arg?Asp

1235 1240 1245

Leu?Lys Asp?Glu?Ile?Val?Arg Leu?Asn?Asp?Ser?Ile Leu?Gln?Ala

1250 1255 1260

Thr?Glu Gln?Arg?Arg?Arg?Ala Glu?Glu?Asn?Ala?Leu Gln?Gln?Lys

1265 1270 1275

Ala?Cys Gly?Ser?Glu?Ile?Met Gln?Lys?Lys?Gln?His Leu?Glu?Ile

1280 1285 1290

Glu?Leu Lys?Gln?Val?Met?Gln Gln?Arg?Ser?Glu?Asp Asn?Ala?Arg

1295 1300 1305

His?Lys Gln?Ser?Leu?Glu?Glu Ala?Ala?Lys?Thr?Ile Gln?Asp?Lys

1310 1315 1320

Asn?Lys Glu?Ile?Glu?Arg?Leu Lys?Ala?Glu?Phe?Gln Glu?Glu?Ala

1325 1330 1335

Lys?Arg Arg?Trp?Glu?Tyr?Glu Asn?Glu?Leu?Ser?Lys Val?Arg?Asn

1340 1345 1350

Asn?Tyr Asp?Glu?Glu?Ile?Ile Ser?Leu?Lys?Asn?Gln Phe?Glu?Thr

1355 1360 1365

Glu?Ile Asn?Ile?Thr?Lys?Thr Thr?Ile?His?Gln?Leu Thr?Met?Gln

1370 1375 1380

Lys?Glu Glu?Asp?Thr?Ser?Gly Tyr?Arg?Ala?Gln?Ile Asp?Asn?Leu

1385 1390 1395

Thr?Arg Glu?Asn?Arg?Ser?Leu Ser?Glu?Glu?Ile?Lys Arg?Leu?Lys

1400 1405 1410

Asn?Thr Leu?Thr?Gln?Thr?Thr Glu?Asn?Leu?Arg?Arg Val?Glu?Glu

1415 1420 1425

Asp?Ile Gln?Gln?Gln?Lys?Ala Thr?Gly?Ser?Glu?Val Ser?Gln?Arg

1430 1435 1440

Lys?Gln Gln?Leu?Glu?Val?Glu Leu?Arg?Gln?Val?Thr Gln?Met?Arg

1445 1450 1455

Thr?Glu Glu?Ser?Val?Arg?Tyr Lys?Gln?Ser?Leu?Asp Asp?Ala?Ala

1460 1465 1470

Lys?Thr Ile?Gln?Asp?Lys?Asn Lys?Glu?Ile?Glu?Arg Leu?Lys?Gln

1475 1480 1485

Leu?Ile Asp?Lys?Glu?Thr?Asn Asp?Arg?Lys?Cys?Leu Glu?Asp?Glu

1490 1495 1500

Asn?Ala Arg?Leu?Gln?Arg?Val Gln?Tyr?Asp?Leu?Gln Lys?Ala?Asn

1505 1510 1515

Ser?Ser Ala?Thr?Glu?Thr?Ile Asn?Lys?Leu?Lys?Val Gln?Glu?Gln

1520 1525 1530

Glu?Leu Thr?Arg?Leu?Arg?Ile Asp?Tyr?Glu?Arg?Val Ser?Gln?Glu

1535 1540 1545

Arg?Thr Val?Lys?Asp?Gln?Asp Ile?Thr?Arg?Phe?Gln Asn?Ser?Leu

1550 1555 1560

Lys?Glu Leu?Gln?Leu?Gln?Lys Gln?Lys?Val?Glu?Glu Glu?Leu?Asn

1565 1570 1575

Arg?Leu Lys?Arg?Thr?Ala?Ser Glu?Asp?Ser?Cys?Lys Arg?Lys?Lys

1580 1585 1590

Leu?Glu Glu?Glu?Leu?Glu?Gly Met?Arg?Arg?Ser?Leu Lys?Glu?Gln

1595 1600 1605

Ala?Ile Lys?Ile?Thr?Asn?Leu Thr?Gln?Gln?Leu?Glu Gln?Ala?Ser

1610 1615 1620

Ile?Val Lys?Lys?Arg?Ser?Glu Asp?Asp?Leu?Arg?Gln Gln?Arg?Asp

1625 1630 1635

Val?Leu Asp?Gly?His?Leu?Arg Glu?Lys?Gln?Arg?Thr Gln?Glu?Glu

1640 1645 1650

Leu?Arg Arg?Leu?Ser?Ser?Glu Val?Glu?Ala?Leu?Arg Arg?Gln?Leu

1655 1660 1665

Leu?Gln Glu?Gln?Glu?Ser?Val Lys?Gln?Ala?His?Leu Arg?Asn?Glu

1670 1675 1680

His?Phe Gln?Lys?Ala?Ile?Glu Asp?Lys?Ser?Arg?Ser Leu?Asn?Glu

1685 1690 1695

Ser?Lys Ile?Glu?Ile?Glu?Arg Leu?Gln?Ser?Leu?Thr Glu?Asn?Leu

1700 1705 1710

Thr?Lys Glu?His?Leu?Met?Leu Glu?Glu?Glu?Leu?Arg Asn?Leu?Arg

1715 1720 1725

Leu?Glu Tyr?Asp?Asp?Leu?Arg Arg?Gly?Arg?Ser?Glu Ala?Asp?Ser

1730 1735 1740

Asp?Lys Asn?Ala?Thr?Ile?Leu Glu?Leu?Arg?Ser?Gln Leu?Gln?Ile

1745 1750 1755

Ser?Asn Asn?Arg?Thr?Leu?Glu Leu?Gln?Gly?Leu?Ile Asn?Asp?Leu

1760 1765 1770

Gln?Arg Glu?Arg?Glu?Asn?Leu Arg?Gln?Glu?Ile?Glu Lys?Phe?Gln

1775 1780 1785

Lys?Gln Ala?Leu?Glu?Ala?Ser Asn?Arg?Ile?Gln?Glu Ser?Lys?Asn

1790 1795 1800

Gln?Cys Thr?Gln?Val?Val?Gln Glu?Arg?Glu?Ser?Leu Leu?Val?Lys

1805 1810 1815

Ile?Lys Val?Leu?Glu?Gln?Asp Lys?Ala?Arg?Leu?Gln Arg?Leu?Glu

1820 1825 1830

Asp?Glu Leu?Asn?Arg?Ala?Lys Ser?Thr?Leu?Glu?Ala Glu?Thr?Arg

1835 1840 1845

Val?Lys Gln?Arg?Leu?Glu?Cys Glu?Lys?Gln?Gln?Ile Gln?Asn?Asp

1850 1855 1860

Leu?Asn Gln?Trp?Lys?Thr?Gln Tyr?Ser?Arg?Lys?Glu Glu?Ala?Ile

1865 1870 1875

Arg?Lys Ile?Glu?Ser?Glu?Arg Glu?Lys?Ser?Glu?Arg Glu?Lys?Asn

1880 1885 1890

Ser?Leu Arg?Ser?Glu?Ile?Glu Arg?Leu?Gln?Ala?Glu Ile?Lys?Arg

1895 1900 1905

Ile?Glu Glu?Arg?Cys?Arg?Arg Lys?Leu?Glu?Asp?Ser Thr?Arg?Glu

1910 1915 1920

Thr?Gln Ser?Gln?Leu?Glu?Thr Glu?Arg?Ser?Arg?Tyr Gln?Arg?Glu

1925 1930 1935

Ile?Asp Lys?Leu?Arg?Gln?Arg Pro?Tyr?Gly?Ser?His Arg?Glu?Thr

1940 1945 1950

Gln?Thr Glu?Cys?Glu?Trp?Thr Val?Asp?Thr?Ser?Lys Leu?Val?Phe

1955 1960 1965

Asp?Gly Leu?Arg?Lys?Lys?Val Thr?Ala?Met?Gln?Leu Tyr?Glu?Cys

1970 1975 1980

Gln?Leu Ile?Asp?Lys?Thr?Thr Leu?Asp?Lys?Leu?Leu Lys?Gly?Lys

1985 1990 1995

Lys?Ser Val?Glu?Glu?Val?Ala Ser?Glu?Ile?Gln?Pro Phe?Leu?Arg

2000 2005 2010

Gly?Ala Gly?Ser?Ile?Ala?Gly Ala?Ser?Ala?Ser?Pro Lys?Glu?Lys

2015 2020 2025

Tyr?Ser Leu?Val?Glu?Ala?Lys Arg?Lys?Lys?Leu?Ile Ser?Pro?Glu

2030 2035 2040

Ser?Thr Val?Met?Leu?Leu?Glu Ala?Gln?Ala?Ala?Thr Gly?Gly?Ile

2045 2050 2055

Ile?Asp Pro?His?Arg?Asn?Glu Lys?Leu?Thr?Val?Asp Ser?Ala?Ile

2060 2065 2070

Ala?Arg Asp?Leu?Ile?Asp?Phe Asp?Asp?Arg?Gln?Gln Ile?Tyr?Ala

2075 2080 2085

Ala?Glu Lys?Ala?Ile?Thr?Gly Phe?Asp?Asp?Pro?Phe Ser?Gly?Lys

2090 2095 2100

Thr?Val Ser?Val?Ser?Glu?Ala Ile?Lys?Lys?Asn?Leu Ile?Asp?Arg

2105 2110 2115

Glu?Thr Gly?Met?Arg?Leu?Leu Glu?Ala?Gln?Ile?Ala Ser?Gly?Gly

2120 2125 2130

Val?Val Asp?Pro?Val?Asn?Ser Val?Phe?Leu?Pro?Lys Asp?Val?Ala

2135 2140 2145

Leu?Ala Arg?Gly?Leu?Ile?Asp Arg?Asp?Leu?Tyr?Arg Ser?Leu?Asn

2150 2155 2160

Asp?Pro Arg?Asp?Ser?Gln?Lys Asn?Phe?Val?Asp?Pro Val?Thr?Lys

2165 2170 2175

Lys?Lys Val?Ser?Tyr?Val?Gln Leu?Lys?Glu?Arg?Cys Arg?Ile?Glu

2180 2185 2190

Pro?His Thr?Gly?Leu?Leu?Leu Leu?Ser?Val?Gln?Lys Arg?Ser?Met

2195 2200 2205

Ser?Phe Gln?Gly?Ile?Arg?Gln Pro?Val?Thr?Val?Thr Glu?Leu?Val

2210 2215 2220

Asp?Ser Gly?Ile?Leu?Arg?Pro Ser?Thr?Val?Asn?Glu Leu?Glu?Ser

2225 2230 2235

Gly?Gln Ile?Ser?Tyr?Asp?Glu Val?Gly?Glu?Arg?Ile Lys?Asp?Phe

2240 2245 2250

Leu?Gln Gly?Ser?Ser?Cys?Ile Ala?Gly?Ile?Tyr?Asn Glu?Thr?Thr

2255 2260 2265

Lys?Gln Lys?Leu?Gly?Ile?Tyr Glu?Ala?Met?Lys?Ile Gly?Leu?Val

2270 2275 2280

Arg?Pro Gly?Thr?Ala?Leu?Glu Leu?Leu?Glu?Ala?Gln Ala?Ala?Thr

2285 2290 2295

Gly?Phe Ile?Val?Asp?Pro?Val Ser?Asn?Leu?Arg?Leu Pro?Val?Glu

2300 2305 2310

Glu?Ala Tyr?Lys?Arg?Gly?Leu Val?Gly?Ile?Glu?Phe Lys?Glu?Lys

2315 2320 2325

Leu?Leu Ser?Ala?Glu?Arg?Ala Val?Thr?Gly?Tyr?Asn Asp?Pro?Glu

2330 2335 2340

Thr?Gly Asn?Ile?Ile?Ser?Leu Phe?Gln?Ala?Met?Asn Lys?Glu?Leu

2345 2350 2355

Ile?Glu Lys?Gly?His?Gly?Ile Arg?Leu?Leu?Glu?Ala Gln?Ile?Ala

2360 2365 2370

Thr?Gly Gly?Ile?Ile?Asp?Pro Lys?Glu?Ser?His?Arg Leu?Pro?Val

2375 2380 2385

Asp?Ile Ala?Tyr?Lys?Arg?Gly Tyr?Phe?Asn?Glu?Glu Leu?Ser?Glu

2390 2395 2400

Ile?Leu Ser?Asp?Pro?Ser?Asp Asp?Thr?Lys?Gly?Phe Phe?Asp?Pro

2405 2410 2415

Asn?Thr Glu?Glu?Asn?Leu?Thr Tyr?Leu?Gln?Leu?Lys Glu?Arg?Cys

2420 2425 2430

Ile?Lys Asp?Glu?Glu?Thr?Gly Leu?Cys?Leu?Leu?Pro Leu?Lys?Glu

2435 2440 2445

Lys?Lys Lys?Gln?Val?Gln?Thr Ser?Gln?Lys?Asn?Thr Leu?Arg?Lys

2450 2455 2460

Arg?Arg Val?Val?Ile?Val?Asp Pro?Glu?Thr?Asn?Lys Glu?Met?Ser

2465 2470 2475

Val?Gln Glu?Ala?Tyr?Lys?Lys Gly?Leu?Ile?Asp?Tyr Glu?Thr?Phe

2480 2485 2490

Lys?Glu Leu?Cys?Glu?Gln?Glu Cys?Glu?Trp?Glu?Glu Ile?Thr?Ile

2495 2500 2505

Thr?Gly Ser?Asp?Gly?Ser?Thr Arg?Val?Val?Leu?Val Asp?Arg?Lys

2510 2515 2520

Thr?Gly Ser?Gln?Tyr?Asp?Ile Gln?Asp?Ala?Ile?Asp Lys?Gly?Leu

2525 2530 2535

Val?Asp Arg?Lys?Phe?Phe?Asp Gln?Tyr?Arg?Ser?Gly Ser?Leu?Ser

2540 2545 2550

Leu?Thr Gln?Phe?Ala?Asp?Met Ile?Ser?Leu?Lys?Asn Gly?Val?Gly

2555 2560 2565

Thr?Ser Ser?Ser?Met?Gly?Ser Gly?Val?Ser?Asp?Asp Val?Phe?Ser

2570 2575 2580

Ser?Ser Arg?His?Glu?Ser?Val Ser?Lys?Ile?Ser?Thr Ile?Ser?Ser

2585 2590 2595

Val?Arg Asn?Leu?Thr?Ile?Arg Ser?Ser?Ser?Phe?Ser Asp?Thr?Leu

2600 2605 2610

Glu?Glu Ser?Ser?Pro?Ile?Ala Ala?Ile?Phe?Asp?Thr Glu?Asn?Leu

2615 2620 2625

Glu?Lys Ile?Ser?Ile?Thr?Glu Gly?Ile?Glu?Arg?Gly Ile?Val?Asp

2630 2635 2640

Ser?Ile Thr?Gly?Gln?Arg?Leu Leu?Glu?Ala?Gln?Ala Cys?Thr?Gly

2645 2650 2655

Gly?Ile Ile?His?Pro?Thr?Thr Gly?Gln?Lys?Leu?Ser Leu?Gln?Asp

2660 2665 2670

Ala?Val Ser?Gln?Gly?Val?Ile Asp?Gln?Asp?Met?Ala Thr?Ser?Val

2675 2680 2685

Lys?Pro Ala?Gln?Lys?Ala?Phe Ile?Gly?Phe?Glu?Gly Val?Lys?Gly

2690 2695 2700

Lys?Lys Lys?Met?Ser?Ala?Ala Glu?Ala?Val?Lys?Glu Lys?Trp?Leu

2705 2710 2715

Pro?Tyr Glu?Ala?Gly?Gln?Arg Phe?Leu?Glu?Phe?Gln Tyr?Leu?Thr

2720 2725 2730

Gly?Gly Leu?Val?Asp?Pro?Glu Val?His?Gly?Arg?Ile Ser?Thr?Glu

2735 2740 2745

Glu?Ala Ile?Arg?Lys?Gly?Phe Ile?Asp?Gly?Arg?Ala Ala?Gln?Arg

2750 2755 2760

Leu?Gln Asp?Thr?Ser?Ser?Tyr Ala?Lys?Ile?Leu?Thr Cys?Pro?Lys

2765 2770 2775

Thr?Lys Leu?Lys?Ile?Ser?Tyr Lys?Asp?Ala?Ile?Asn Arg?Ser?Met

2780 2785 2790

Val?Glu Asp?Ile?Thr?Gly?Leu Arg?Leu?Leu?Glu?Ala Ala?Ser?Val

2795 2800 2805

Ser?Ser Lys?Gly?Leu?Pro?Ser Pro?Tyr?Asn?Met?Ser Ser?Ala?Pro

2810 2815 2820

Gly?Ser Arg?Ser?Gly?Ser?Arg Ser?Gly?Ser?Arg?Ser Gly?Ser?Arg

2825 2830 2835

Ser?Gly Ser?Arg?Ser?Gly?Ser Arg?Arg?Gly?Ser?Phe Asp?Ala?Thr

2840 2845 2850

Gly?Asn Ser?Ser?Tyr?Ser?Tyr Ser?Tyr?Ser?Phe?Ser Ser?Ser?Ser

2855 2860 2865

Ile?Gly?His

2870

Claims

1. be used to handle the make-up composition that contains the keratin thing, comprise at least a keratin-binding polypeptides sequence (i) in the cosmetic compatible media, wherein peptide sequence (i) comprises at least a following peptide sequence:

(a) the 2193rd to 2481 of peptide sequence SEQ ID NO:1 the,

(b) the 2606th to 2871 of peptide sequence SEQ ID NO:1 the,

(c) compare with (a), go up to the adorned peptide sequence of 60% aminoacid,

(d) compare with (b), go up to the adorned peptide sequence of 50% aminoacid, condition be peptide sequence (c) or keratin (d) in conjunction with equal peptide sequence (a) or (b) shown value at least 10%.

2. make-up composition according to claim 1, wherein peptide sequence (i) has binding affinity to human hair keratin, fingernail keratin or skin keratin.

3. make-up composition according to claim 1, comprising is the peptide sequence (i) of 0.01% to 30% amount by weight.

4. make-up composition according to claim 1 wherein except that peptide sequence (i), also comprises at least a cosmetic active component.

5. make-up composition according to claim 4, the active component of wherein making up are selected from natural or synthetic polymer, pigment, humidizer, oil, wax, enzyme, mineral, vitamin, opacifier, dyestuff, spice, antioxidant and antiseptic.

6. the purposes of each described make-up composition in improving the hair cardability among the claim 1-5.

7. the purposes of each described make-up composition in improving Hairsetting among the claim 1-5.

8. keratin-binding polypeptides sequence (i) is in the purposes of preparation in the make-up composition, and described make-up composition is used to regulate skin effects, and wherein peptide sequence (i) as defined in claim 1.

Among the claim 1-5 each described make-up composition in the purposes of preparation in skin, fingernail and the hair make-up composition.

10. be used to handle the pharmaceutical composition that contains the keratin thing, comprise at least a keratin-binding polypeptides sequence (i) in the medicine compatible media,

Wherein peptide sequence (i) comprises at least a following peptide sequence:

(a) the 2269th to 2508 of peptide sequence SEQ ID NO:1 the,

(b) the 2606th to 2871 of peptide sequence SEQ ID NO:1 the,

(c) compare with (a), go up to the adorned peptide sequence of 70% aminoacid,

(d) compare with (b), go up to the adorned peptide sequence of 70% aminoacid,

Condition be peptide sequence (c) or keratin (d) in conjunction with equal peptide sequence (a) or (b) shown value at least 10%.

11. keratin-binding effector molecules, by:

(i) at least a have the peptide sequence of binding affinity to keratin,

The (ii) effector molecule that is connected with peptide sequence (i) and non-natural

Form,

Wherein peptide sequence (i) comprises at least a following peptide sequence:

(a) the 2269th to 2508 of peptide sequence SEQ ID NO:1 the,

(b) the 2606th to 2871 of peptide sequence SEQ ID NO:1 the,

(c) compare with (a), go up to the adorned peptide sequence of 70% aminoacid,

(d) compare with (b), go up to the adorned peptide sequence of 70% aminoacid,

12. keratin-binding effector molecules according to claim 11, wherein peptide sequence (i) has binding affinity to the keratin of Crinis Carbonisatus, fingernail or skin.

13. keratin-binding effector molecules according to claim 11, wherein effector molecule (ii) comprises peptide sequence.

14. keratin-binding effector molecules according to claim 11, wherein effector molecule (ii) has enzymatic activity.

15. keratin-binding effector molecules according to claim 11, wherein effector molecule (ii) is dyestuff or dye component.

16. keratin-binding effector molecules according to claim 11, wherein effector molecule (ii) is the ultraviolet filter agent.

17. keratin-binding effector molecules according to claim 11, wherein effector molecule (ii) is an antioxidant.

18. keratin-binding effector molecules according to claim 11, wherein effector molecule (ii) is a carotenoid.

19. keratin-binding effector molecules according to claim 11, wherein effector molecule (ii) is antifungal, insecticide or Biocide.

20. keratin-binding effector molecules according to claim 11, wherein effector molecule (ii) is vitamin or provitamin.

21. the purposes of the described keratin-binding effector molecules of each of claim 11-20 in preparation skin, fingernail and hair make-up composition.

22. according to the keratin-binding effector molecules of claim 11, wherein effector molecule (ii) is connected in peptide sequence (i) by covalent bond.

23. keratin-binding effector molecules according to claim 11, two chemical functional groups that wherein utilize polypeptide (i) and effector molecule to exist already in (ii) (ii) are covalently attached to effector molecule the functional group of side chain, C-terminal or the N-terminal of polypeptide (i).

24. keratin-binding effector molecules according to claim 11 wherein (ii) is covalently attached to one or more effector molecules the functional group of side chain, C-terminal or the N-terminal of polypeptide (i) by dual functional at least joint.

25. keratin-binding effector molecules according to claim 11 wherein (ii) and between the polypeptide (i) mixes spacer element at effector molecule.

26. according to claim 24 or 25 described keratin-binding effector molecules, it at effector molecule (ii) and have the potential cleavage site of protease, lipase, esterase, phosphatase or hydrolytic enzyme between the polypeptide (i).

27. according to claim 24 or 25 described keratin-binding effector molecules, it at effector molecule (ii) and have another peptide sequence that allows to be easy to purified fusion protein between the polypeptide (i).

28. be used to prepare the method for the described keratin-binding effector molecules of claim 11, comprise utilize joint with at least one effector molecule (ii) with keratin-binding polypeptides sequence (i) coupling.