CN1926999A - Natural plant pesticide, preparation method and application thereof - Google Patents
Natural plant pesticide, preparation method and application thereof Download PDFInfo
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- CN1926999A CN1926999A CN 200610153595 CN200610153595A CN1926999A CN 1926999 A CN1926999 A CN 1926999A CN 200610153595 CN200610153595 CN 200610153595 CN 200610153595 A CN200610153595 A CN 200610153595A CN 1926999 A CN1926999 A CN 1926999A
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Abstract
The invention discloses a natural botanical insecticides and its preparation methods, belonging to the field of plants pesticide, according to the volume of the praeparatum the insecticides contains 1mg/mL~10mg/mL active substance patchoulenone or 5mg/mL~40mg/mL of patchouli oil. In the preparation methods, patchoulenone emulsion is dispersing directly and uniformly the patchoulenone in the 5%span-20 solution according to the match; patchouli oil emulsion is dispersing directly and uniformly the patchouli in 6% Tween-80 solution according to the match.
Description
Technical field
The present invention relates to a kind of plant pesticide, refer in particular to a kind of natural botanical insecticide that is used for crop pest control, the invention still further relates to the preparation method of this insectofungicide and the application on agricultural.
Background technology
Agricultural chemicals is the assurance agricultural foison, improves human food's supply, improves human living standard's requisite valuable cargo.For this reason, many in the world countries drops into suitable human and material resources and is devoted to some global big chemical enterprises in the exploitation, particularly developed country of novel pesticide, puts one's ass on the line especially and carries out the development of novel pesticide.Now, developing a new chemical pesticide often needs 7 to 8,000 ten thousand dollars, lasts about 10 years, screens up to ten thousand compounds and begins to obtain a novel pesticide with commercial value.Yet a large amount of uses along with chemical pesticide have brought all drawbacks to the mankind, such as the drug-fast generation of pest, problems such as residual toxicity and environmental pollution.At present, many high toxicities, high residue, the persistent pesticide use that has been under an embargo.Deep variation has also taken place to the understanding and the expectation of agricultural chemicals in people.People to the target diversion of novel pesticide exploitation to the pest high activity, to non-target organism safety, low-residual, the easy agricultural chemicals of degraded.Therefore, the exploitation novel pesticide is very an important approach and a breach from natural materials.
In natural materials, plant is the natural treasure-house of bioactive compound.Found at present to have pesticide activity just reach 5000 surplus kind, the secondary metabolites of its generation is above 400000 kinds, the amino acid of the rare class of most compounds wherein such as terpene, alkaloid, flavonoids, steroidal, phenols, uniqueness and polysaccharose etc. all have desinsection or antibacterial activity, what this natural active matter had directly can agricultural chemicals, therefore the lead compound that can be used as that has makes the compound with pesticide activity by structure of modification, and developing plant resources, to be used for the prospect of PCO very wide.Ancient Times in China medicine scholar has accumulated abundant tcm clinical knowledge in long-term practice, for the screening operation of studying Farm chemical of Chinese berbs provides very strong specific aim.Has very strong realistic meaning as breach research Farm chemical of Chinese berbs active component, searching pesticide activity parent, innovation novel pesticide.
Summary of the invention
One of them purpose of the present invention be to provide a kind of aboundresources, cost low, to the pest high activity, to non-target organism safety, low-residual, easy a kind of natural botanical insecticide of degraded.
Another object of the present invention is to provide a kind of preparation method who states plant pest killing and sterilizing agent.
Last purpose of the present invention is achieved in that a kind of natural botanical insecticide, wherein contains the active substance Pogostone of 1mg/mL～10mg/mL by the stereometer of preparation.
Preparation described in above-mentioned a kind of natural botanical insecticide is good with emulsion, and it is stable, convenient easy-to-use to make formulation like this.
The active substance Pogostone replaces with Herba Pogostemonis essential oil described in above-mentioned a kind of natural botanical insecticide, and the content of Herba Pogostemonis essential oil is 2.5mg/mL～40mg/mL; Described preparation is good with emulsion, can guarantee that Herba Pogostemonis essential oil is uniformly dispersed, stablizes, the convenient use.
Above-mentioned a kind of natural botanical insecticide is with a wide range of applications in agricultural, plant husbandry, storage.
Back of the present invention one purpose is achieved in that a kind of preparation method of natural botanical insecticide, and wherein aqua is that Pogostone directly is dispersed in the 5% Arlacel-20 solution by proportioning.Emulsion agent is that Herba Pogostemonis essential oil directly is dispersed in the 6% Tween-80 solution by proportioning.
The present invention compared with prior art has following advantage:
(1) Pogostemon cablin has long medication history as a kind of important botanical herbs, and is safe in utilization.
(2) Pogostemon cablin in Guangzhou in Guangdong Province, ground such as Zhaoqing, Zhanjiang distribute wide, reserves are big, also there is bigger resource in Hainan of adjoining, Guangxi, this Application and Development for this plant provides abundant raw.
(3) laboratory test confirms that Pogostone and Herba Pogostemonis essential oil have control santal white butterfly and lichee leaf roller invasion and attack effect, imported cabbageworm and diamondback moth larvae had food refusal preferably and toxic action, prodenia litura there is stronger food refusal effect, the various plants disease fungus is all had in various degree inhibitory action.
(4) extracting method of Pogostone and Herba Pogostemonis essential oil is simple and easy, technical maturity.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail, but does not constitute any limitation of the invention.Pogostone of the present invention is to separate from Herba Pogostemonis essential oil to obtain, and Herba Pogostemonis essential oil is by root, the rhizome of traditional traditional medicine volatile oil extracting method from the plant Pogostemon cablin, extracts in stem and the leaf.
Embodiment 1
Herba Pogostemonis essential oil is to food refusal, the deterrent effect of santal white butterfly and lichee leaf roller
1.1 for trying essential oil: Herba Pogostemonis essential oil is carried certainly for this research institute, and every milliliter contains crude drug 50g approximately.
1.2 for the examination insect
Santal white butterfly (Delias aglaia L.): adopt back the santal larva of hatching just from this research institute living collection, put into big plastic casing, feed, treat to supply when larva grew to for 5 ages examination with the tender santal leaf of children.
The brown belt length leaf roller of lichee (Homona coffearia Nietner): adopt back the brown belt length leaf roller of the lichee larva of hatching just from the orchard, Zengcheng, put into big plastic casing, feed, treat to supply when larva grew to for 5 ages examination with the tender lychee leaf of children.
1.3 the food refusal effect is measured
The medicament preparation: with 6% Tween-80 solution is solvent, respectively the Herba Pogostemonis essential oil doubling dilution is become 2.5mg/mL, 5mg/mL, 10mg/mL, 20mg/mL, five gradient concentrations of 40mg/mL.
The selectivity antifeedant activity of feeding is measured: cut-off directly is the culture dish of 9cm, preserves moisture with wet filter paper at the bottom of the culture dish.In same culture dish, put into and handle the leaf dish and contrast each 2 pieces in leaf dish, cross arrangement.Insert the confession examination larva of 1 hungry 4h, establish 6 repetitions.Behind the 24h remaining blade is taken out, measure the quilt of leaf dish with squared paper and get the food area.
The non-selective antifeedant activity of feeding is measured: cut-off directly is the culture dish of 9cm, preserves moisture with wet filter paper at the bottom of the culture dish.In same culture dish, put into and handle the leaf dish and contrast each 4 pieces in leaf dish.Contrast leaf dish is then put into another culture dish.All insert the confession examination larva of 1 hungry 4h, establish 6 repetitions.Behind the 24h remaining blade is taken out, measure the quilt of leaf dish with squared paper and get the food area.
The calculating of food refusal rate: selectivity food refusal rate=(S
Ck-S)/(S
Ck+ S); Non-selective food refusal rate=(S
Ck-S)/S
CkWherein: S
CkOn average get the food area for contrast leaf dish; S on average gets the food area for what handle the leaf dish.Utilize SPSS software that data are carried out statistical analysis, multiple ratio adopts Duncan ' s duncan's new multiple range method.
1.4 deterrent effect is measured
The santal white butterfly avoid keep away the test be the branch of adopting back fresh and tender no worm, with glass triangle bottle sprayer test solution is sprayed thereon equably, in the 250ml beaker, add running water, rim of a cup is put a circular hard paper, the place's of keeping to the side relative direction is made a call to two holes, get one and handle branch and a contrast branch (only spraying 6% Tween-80 solution), insert in the beaker in the hole of passing on the scraps of paper.Clip contains several in the blade of a great deal of santal white butterfly larva, place in the middle of the scraps of paper, by the time blade withered after, the santal white butterfly just can be fled from by larva automatically.Behind the certain hour, judge the deterrent effect of this processing to the santal white butterfly with the santal white butterfly larva number of handling and contrast land on the branch.Avoiding the rate of keeping away calculates as follows:
The lichee leaf roller avoids that to keep away test be to be divided into two halves with rectangle 5 * 10cm filter paper, and half is for handling, and half is for contrasting.Essential oil is got the even spread of 1ml on filter paper after becoming variable concentrations with 6% Tween-80 solution dilution, makes the soup deposition on the filter paper reach 0.5mg/cm respectively
2, 1mg/cm
2, 2mg/cm
2, 4mg/cm
2, 8mg/cm
21ml6% Tween-80 solution is dripped in contrast, will handle and contrast two half of filter paper and couple together with transparent adhesive tape at reverse side, puts into the rectangular plastic magazine.20 lichee leaf rollers are inserted in the plastic casing, be allowed to condition on the filter paper and freely creep.Each checks that once contrasting the limit closes the borer population of handling the limit distribution after 4 hours and 24 o'clock.Avoiding the rate of keeping away calculates as follows:
1.5 the non-selective food refusal effect of feeding
The variable concentrations Herba Pogostemonis essential oil sees Table 1 to the non-selective food refusal exercising result of santal white butterfly and lichee leaf roller larva.
Table 1 Herba Pogostemonis essential oil is to the non-selective food refusal effect of santal white butterfly and lichee leaf roller
| Concentration of treatment (mg/mL) | The santal white butterfly | The lichee leaf roller | ||||||
| Get food leaf area (mm 2/ head) | Food refusal rate (%) | Get food leaf area (mm 2/ head) | Food refusal rate (%) | |||||
| 24h | 48h | 24h | 48h | 24h | 48h | 24h | 48h | |
| CK | 64.76±11.78a | 82.43±15.58a | 335.59±88.89a | 313.51±129.27a | ||||
| 2.5 | 0b | 0b | 100 | 100 | 165.32±23.45b | 156.83±17.52b | 51 | 53 |
| 5 | 0b | 0b | 100 | 100 | 105.98±26.98bc | 117.75±15.58bc | 68 | 71 |
| 10 | 0b | 0b | 100 | 100 | 13.25±13.25c | 39.74±39.74c | 96 | 91 |
| 20 | 0b | 0b | 100 | 100 | 4.42±4.42d | 4.42±4.42d | 96 | 98 |
| 40 | 0b | 0b | 100 | 100 | 0e | 0e | 100 | 100 |
As known from Table 1, Herba Pogostemonis essential oil has food refusal effect preferably to santal white butterfly and lichee leaf roller larva, and the antifeedant activity of 24h and 48h all raises with the raising of working concentration.Wherein more responsive with santal white butterfly larva, the food refusal rate of each concentration all is 100%; 2.5mg/mL-40mg/mL concentration is better to lichee leaf roller food refusal effect, all more than 90%.Simultaneously can see that the food refusal effect also descends along with the postponement of time, 48h slightly descends than the food refusal rate of 24h.
The food refusal effect 1.6 selectivity is fed
Table 2 Herba Pogostemonis essential oil is to the selectively acting of santal white butterfly and lichee leaf roller
| Chemicals treatment (mg/mL) | The santal white butterfly | The lichee leaf roller | ||
| Get food leaf area (mm 2/ head) | Food refusal rate (%) | Get food leaf area (mm 2/ head) | Food refusal rate (%) | |
| CK | 61.82±35.69 | 100 | 223.08±25.63 | 18 |
| 2.5 | 0 | 154.64±31.46 | ||
| CK | 23.55±11.78 | 100 | 147.19±15.58 | 30 |
| 5 | 0 | 80.32±14.35 | ||
| CK | 47.10±23.55 | 100 | 335.59±10.20 | 39 |
| 10 | 0 | 147.19±62.31 | ||
| CK | 123.64±30.59 | 100 | 347.36±5.89 | 91 |
| 20 | 0 | 13.25±13.25 | ||
| CK | 156.78±25.36 | 100 | 282.60±70.65 | 100 |
| 40 | 0 | 0 | ||
As shown in Table 2, patchouli oil is very strong to the selectivity food refusal effect of santal white butterfly larva, and the above concentration of 2.5mg/mL just can reach 100% food refusal rate.Concentration more than the 20mg/mL is also comparatively responsive to the lichee leaf roller, and the food refusal rate is more than 90%.
1.7 deterrent effect is measured
Table 3 Herba Pogostemonis essential oil is to the deterrent effect of santal white butterfly and lichee leaf roller
| Concentration of treatment (mg/mL) | The santal white butterfly | The lichee leaf roller | ||||||||
| Examination borer population (head) | Observing time (h) | Handle on (head) | Contrast (head) | Avoid the rate of keeping away (%) | Examination borer population (head) | Observing time (h) | Handle on (head) | Contrast (head) | Avoid the rate of keeping away (%) | |
| 2.5 | 20 | 4 | 5.5±1.87 | 8.67±1.63 | 36.56 | 20 | 4 | 5.8±0.93 | 14.4±0.97 | 59.72 |
| 24 | 5.5±1.38 | 7±1.27 | 21.43 | 24 | 8.17±0.48 | 12.2±0.66 | 33.03 | |||
| 5 | 20 | 4 | 4.4±1.14 | 9±1.58 | 51.11 | 20 | 4 | 6.0±0.84 | 14.6±0.87 | 58.90 |
| 24 | 5.4±1.82 | 9.6±2.19 | 43.75 | 24 | 7.0±0.84 | 13.4±0.58 | 47.76 | |||
| 10 | 20 | 4 | 4.4±1.14 | 11.8±1.48 | 62.71 | 20 | 4 | 4.2±0.88 | 16.2±0.58 | 74.07 |
| 24 | 5.2±1.48 | 13.8±1.92 | 62.32 | 24 | 5.2±0.97 | 15.2±0.8 | 65.79 | |||
| 20 | 20 | 4 | 3.2±1.48 | 10±1.87 | 68.00 | 20 | 4 | 1.4±0.51 | 19.2±1.58 | 92.71 |
| 24 | 3.4±1.82 | 9.6±2.19 | 64.58 | 24 | 3.4±0.93 | 17.2±0.8 | 80.23 | |||
| 40 | 20 | 4 | 2.6±1.52 | 13.2±1.48 | 80.30 | 20 | 4 | 1.2±0.58 | 19.4±0.93 | 93.81 |
| 24 | 2.2±1.31 | 10.4±1.30 | 78.85 | 24 | 2.0±0.71 | 18.0±2.02 | 88.89 |
As can be seen from Table 3, the borer population on contrast limit all is higher than the borer population of handling the limit, illustrates that patchouli oil all has deterrent effect to santal white butterfly and lichee leaf roller.
Embodiment 2
Herba Pogostemonis essential oil is to prodenia litura food refusal effect
1.1 for trying essential oil: Herba Pogostemonis essential oil is carried certainly for this research institute, and every milliliter contains crude drug 50g approximately.
1.2 for the examination insect
Prodenia litura (Spodoptera litura Fab.): adopt back the prodenia litura pieces of an egg from the field and be put in the culture dish of preserving moisture, after treating that the ovum hatching is for larva, change in the big glass jar, feed with cassava leaves, the larva of picking neat and consistent is for examination when larva grew to for 3 ages.
1.3 test method
The medicament preparation: with acetone is solvent, respectively the Herba Pogostemonis essential oil doubling dilution is become 2.5mg/mL, 5mg/mL, 10mg/mL, 20mg/mL, five gradient concentrations of 40mg/mL.
Selectivity is fed, and antifeedant activity is measured, the non-selective antifeedant activity of feeding is measured identical with embodiment 1.
1.4 the non-selective food refusal effect of feeding
Table 4 Herba Pogostemonis essential oil is to the non-selective food refusal effect of prodenia litura
| Concentration of treatment (mg/mL) | Get food leaf area (mm 2/ head) | Food refusal rate (%) | ||
| 24h | 48h | 24h | 48h | |
| CK | 335.59±112.18a | 706.5±3.24a | ||
| 2.5 | 301.15±160.05b | 690.61±5.89ab | 18.87±3.42a | 10.45±0.87a |
| 5 | 223.73±86.73c | 667.06±29.44b | 33.51±5.87b | 24.76±1.46b |
| 10 | 123.64±71.38d | 247.28±158.96c | 63.67±9.37c | 65.38±10.79c |
| 20 | 35.33±26.98e | 147.19±58.88d | 89.19±10.56d | 79.84±17.98d |
| 40 | 29.44±15.58ef | 129.53±5.89e | 91.73±7.84e | 82.92±20.56e |
Table 4 shows that each is handled food ingestion comparison illumination and shows decline, and the food refusal rate improves with the raising of handling concentration.48h descends relatively than the food refusal rate of 24h after handling.The food refusal rate is in proper order after handling back 24h and 48h: 40mg/mL＞20mg/mL＞10mg/mL＞5mg/mL＞2.5mg/mL.Concentration AFC in the food refusal of 24h after under non-selective condition, handling
50Be 68.88 μ g/mL (confidence interval is 66.42～71.34 μ g/mL), handle the AFC of back 48h
50Be 94.40 μ g/mL (confidence interval is 91.96～96.85 μ g/mL).
The food refusal effect 1.5 selectivity is fed
Table 5 Herba Pogostemonis essential oil is to prodenia litura selectivity food refusal effect
| Chemicals treatment (mg/mL) | Get food leaf area (mm 2/ head) | Food refusal rate (%) |
| CK | 132.75±13.46 | 45.43±6.87a |
| 2.5 | 50.65±18.35 | |
| CK | 143.73±32.35 | 56.31±9.73b |
| 5 | 41.25±5.89 | |
| CK | 156.15±11.78 | 60.84±12.76c |
| 10 | 38.31±56.02 | |
| CK | 169.53±94.75 | 78.38±4.78d |
| 20 | 21.78±11.78 | |
| CK | 172.43±84.57 | 84.57±13.56e |
| 40 | 10.89±5.89 |
Table 5 shows that the Herba Pogostemonis essential oil of various concentration shows different food refusal effects to the trophic behaviour of Spodoptera litura larvae.In selecting feeding experiment, each concentration all shows very high food refusal rate, and 5 kinds of concentration food refusal rate order are: 40mg/mL＞20mg/mL＞10mg/mL＞5mg/mL＞2.5mg/mL.Concentration AFC in the food refusal of 24h after under selective conditions, handling
50Be 36.23 μ g/mL (confidence interval is 34.19～38.26 μ g/mL).
Embodiment 3
The insecticidal action of Pogostone, Herba Pogostemonis essential oil
1.1 reagent agent: Herba Pogostemonis essential oil is carried certainly for this research institute, and every mL contains crude drug 50g approximately; Pogostone separates preparation by this research department from this essential oil, yield is 0.25%, and purity is more than 98.5%.With 6% Tween-80 solution is solvent, and the Herba Pogostemonis essential oil doubling dilution is become 2.5mg/mL, 5mg/mL, 10mg/mL, 20mg/mL, five gradient concentrations of 40mg/mL; With 5% Arlacel-20 solution is solvent, and the Pogostone doubling dilution is become 1mg/mL, 2mg/mL, 4mg/mL, 8mg/mL, five gradient concentrations of 10mg/mL.
1.2 for the examination insect
Imported cabbageworm (Preris rapae L.): adopt back from this institute living collection teaching practice district the imported cabbageworm pupa and advanced age larva raising, be collected in behind the larvae pupation in the big culture dish, move in the dependent insect cage of long 50cm, wide 60cm, high 80cm during emergence, put into potted plant Jie's indigo plant in the cage, and in cage, flood 10% honey liquid with cotton, supplement the nutrients for adult, allow adult on cabbage mustard, lay eggs, take out behind the 2d, treat that ovum hatches into behind the larva a collection of larva is collected in the big glass jar and feed, treat that it is used for test when growing to 4 instar larvaes with Jie's indigo plant or wild cabbage.
Diamond-back moth (Plutella xylostella L.): fetch high instar larvae from the Agricultural University Of South China insectary and breed at indoor feeding, treat to collect when it is pupated pupa in culture dish, connect culture dish after the emergence and put into the little dependent insect cage of long 50cm, wide 60cm, high 80cm together, be placed with the plastic casing of long 30cm, wide 20cm, high 5cm in the cage, it is the adult extra-nutrition that the interior kind of basin has the tender flower stalk seedling of 2 leaf sizes and 10% honey, allow adult on potted plant tender flower stalk seedling, lay eggs, treat to supply when larva grew to for 3 ages examination.
1.3 test method
Selectivity feeds that antifeedant activity is measured, the non-selective antifeedant activity of feeding measure identical with embodiment 1, the test method(s) of tagging use microsyringe with the sample drop on the chest backboard of examination worm, try the lethality of worm through back inspection after a while.Corrected mortality is tried to achieve by the Abbott formula: corrected mortality (%)=(handling lethality-contrast lethality) * 100/ (1-contrasts lethality).Utilize Excel software to calculate the toxicity regression curve and the LC of variable concentrations patchouli oil and Pogostone
50Value.
1.4 Herba Pogostemonis essential oil is to imported cabbageworm and the non-selective food refusal effect of diamondback moth larvae
Table 6 Herba Pogostemonis essential oil is to imported cabbageworm and the non-selective food refusal effect of diamondback moth larvae
| Concentration of treatment (mg/mL) | Imported cabbageworm | Diamond-back moth | ||||||
| Get food leaf area (mm 2/ head) | Food refusal rate (%) | Get food leaf area (mm 2/ head) | Food refusal rate (%) | |||||
| 24h | 48h | 24h | 48h | 24h | 48h | 24h | 48h | |
| CK | 706.5±20.34a | 706.5±20.34a | 198.96±10.19a | 247.48±20.40a | ||||
| 2.5 | 365.68±32.42b | 447.45±41.21b | 48 | 37 | 140.35±16.24ab | 186.73±20.26ab | 29 | 25 |
| 5 | 300.26±50.99c | 405.45±21.28b | 58 | 43 | 110.65±20.40b | 140.09±32.78b | 44 | 43 |
| 10 | 123.64±26.98d | 135.41±15.58c | 83 | 81 | 94.82±7.36bc | 127.82±17.19bc | 53 | 49 |
| 20 | 0e | 13.25±3.13d | 100 | 98 | 81.03±8.52c | 109.76±11.31c | 59 | 59 |
| 40 | 0e | 8.83±2.75d | 100 | 99 | 65.67±2.49c | 83.88±9.76c | 67 | 66 |
Table 6 shows: Herba Pogostemonis essential oil all has food refusal effect preferably to imported cabbageworm and diamondback moth larvae, and the antifeedant activity of 24h and 48h all raises with the raising of working concentration, and As time goes on the food refusal effect descends, and 48h slightly descends than the food refusal rate of 24h.
1.5 Herba Pogostemonis essential oil is to imported cabbageworm and diamondback moth larvae selectivity food refusal effect
Table 7 Herba Pogostemonis essential oil is to imported cabbageworm and diamondback moth larvae selectivity food refusal effect
| Chemicals treatment (mg/mL) | Imported cabbageworm | Diamond-back moth | ||
| Get food leaf area (mm 2/ head) | Food refusal rate (%) | Get food leaf area (mm 2/ head) | Food refusal rate (%) | |
| CK | 634.24±60.57 | 74 | 280.65±53.42 | 28 |
| 2.5 | 95.14±20.44 | 156.85±35.29 | ||
| CK | 605.98±70.65 | 100 | 256.38±32.12 | 31 |
| 5 | 0 | 134.25±23.56 | ||
| CK | 730.70±63.09 | 100 | 223.53±32.36 | 44 |
| 10 | 0 | 86.32±12.35 | ||
| CK | 697.82±73.97 | 100 | 265.98±25.68 | 58 |
| 20 | 0 | 68.96±13.56 | ||
| CK | 701.35±42.31 | 100 | 231.68±43.21 | 76 |
| 40 | 0 | 32.49±15.68 | ||
Table 7 shows: Herba Pogostemonis essential oil is more strong to the selectivity food refusal effect of imported cabbageworm and diamondback moth larvae, the above essential oil of 5mg/mL just reaches 100% food refusal rate to cabbage caterpillar, comparatively speaking, food refusal effect to diamond-back moth relatively relaxes, and the food refusal rate of each concentration essential oil is respectively 28%, 31%, 44%, 58%, 76%.
1.6 patchouli oil and Pogostone are to the cabbage caterpillar toxic action
Table 8 patchouli oil and Pogostone are to the cabbage caterpillar toxic action
| Chemicals treatment | Linear regression equation | Correlation coefficient (r) | LC 50(μg/mL) | 95% confidence interval (μ g/mL) | Chi-square value (x 2) |
| Patchouli oil | Y＝1.9056x+1.1541 | 0.9934 | 104.28 | 102.17～106.40 | 0.2293 |
| Pogostone | Y＝1.849x+2.2119 | 0.9767 | 32.20 | 29.94～34.46 | 0.2631 |
Table 8 is the result show, Herba Pogostemonis essential oil and Pogostone are all very strong to the toxic action of cabbage caterpillar, LC
50Be respectively 104.28 μ g/mL and 32.20 μ g/mL.
1.7 Pogostone is to imported cabbageworm and the non-selective food refusal effect of diamondback moth larvae
Table 9 Pogostone is to imported cabbageworm and diamondback moth larvae selectivity food refusal effect
| Concentration of treatment (mg/mL) | Imported cabbageworm | Diamond-back moth | ||||||
| Get food leaf area (mm 2/ head) | Food refusal rate (%) | Get food leaf area (mm 2/ head) | Food refusal rate (%) | |||||
| 24h | 736.5±20.34a | 24h | 48h | 24h | 48h | 24h | 48h | |
| CK | 722.31±50.47a | 167.45±21.56b | 276.71±52.33a | 353.25±90.64a | ||||
| 1 | 155.23±22.54b | 85.45±11.52b | 79 | 77 | 88.31±36.32b | 135.33±26.98b | 68 | 62 |
| 2 | 70.17±10.25c | 35.41±5.58c | 90 | 88 | 31.78±11.78c | 47.66±17.66c | 89 | 86 |
| 4 | 28.36±7.56d | 0d | 96 | 95 | 0d | 0d | 100 | 100 |
| 8 | 0e | 0d | 100 | 100 | 0d | 0d | 100 | 100 |
| 10 | 0e | 736.5±20.34a | 100 | 100 | 0d | 100 | 100 | |
As can be seen from Table 9, Pogostone has strong food refusal effect to imported cabbageworm and diamondback moth larvae, and the antifeedant activity of 24h and 48h all raises with the raising of working concentration.The food refusal effect also descends along with the postponement of time, and 48h slightly descends than the food refusal rate of 24h.
1.8 Pogostone is to imported cabbageworm and diamondback moth larvae selectivity food refusal effect
Table 10 Pogostone is to imported cabbageworm and diamondback moth larvae selectivity food refusal effect
| Chemicals treatment (mg/mL) | Dish (blue or green worm) white butterfly | Diamond-back moth | ||
| Get food leaf area (mm 2/ head) | Food refusal rate (%) | Get food leaf area (mm 2/ head) | Food refusal rate (%) | |
| CK | 759.58±57.18 | 80a | 322.44±29.77 | 70a |
| 1 | 84.67±33.73 | 57.07±7.73 | ||
| CK | 721.01±68.14 | 100b | 336.65±30.99 | 93b |
| 2 | 0 | 13.58±7.54 | ||
| CK | 679.53±38.42 | 100b | 313.53±31.76 | 100c |
| 4 | 0 | 0 | ||
| CK | 695.57±85.41 | 100b | 315.18±27.35 | 100c |
| 8 | 0 | 0 | ||
| CK | 701.65±52.74 | 100b | 332.68±33.51 | 100c |
| 10 | 0 | 0 | ||
Table 10 shows: Pogostone is very strong to the selectivity food refusal effect of imported cabbageworm and diamondback moth larvae, and the above concentration of 4mg/mL just can reach 100% food refusal rate.
From the above embodiments as can be seen, with the insectofungicide of Herba Pogostemonis essential oil and Pogostone preparation the insect and the storage insect of vegetables, fruit tree, medicinal plant there is strong food refusal, avoids effect such as keep away, tag, can be used as a kind of novel noresidue, pollution-free pesticide and in agricultural production, plant husbandry, obtains application widely.
Claims (7)
1. a natural botanical insecticide is characterized in that the stereometer by preparation contains the active substance Pogostone of 1mg/mL～10mg/mL.
2. a kind of natural botanical insecticide according to claim 1 is characterized in that described preparation is an emulsion.
3. a kind of natural botanical insecticide according to claim 1 is characterized in that the active substance Pogostone replaces with Herba Pogostemonis essential oil, and the content of Herba Pogostemonis essential oil is 2.5mg/mL～40mg/mL.
4. a kind of natural botanical insecticide according to claim 3 is characterized in that described preparation is an emulsion agent.
5. according to the preparation method of the described a kind of natural botanical insecticide of claim 2, it is characterized in that Pogostone directly is dispersed in the 5% Arlacel-20 solution by proportioning.
6. according to the preparation method of the described a kind of natural botanical insecticide of claim 4, it is characterized in that Herba Pogostemonis essential oil directly is dispersed in the 6% Tween-80 solution by proportioning.
7. according to the application of the described a kind of natural botanical insecticide of claim 1 to 4 in agricultural, plant husbandry, storage.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101427694A (en) * | 2007-11-08 | 2009-05-13 | 冷雪飞 | Natural green biological organic agricultural chemical composition and uses thereof |
| CN101579000B (en) * | 2009-06-23 | 2013-07-10 | 北京农学院 | Ageratum extract, preparation method and application |
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2006
- 2006-09-13 CN CN 200610153595 patent/CN1926999A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101427694A (en) * | 2007-11-08 | 2009-05-13 | 冷雪飞 | Natural green biological organic agricultural chemical composition and uses thereof |
| CN101579000B (en) * | 2009-06-23 | 2013-07-10 | 北京农学院 | Ageratum extract, preparation method and application |
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