CN1844396A - Genes regulating rice tiller angle and their encoded proteins and their applications - Google Patents

Abstract

The invention discloses a section of gene and its protein with controlling rice tillering angulations, and its application. The purpose is that providing a section of gene and its protein with controlling rice tillering angulations, and applying the gene and protein in controlling rice tillering angulations. The gene's cDNA has one of nucleotide sequences as follow: 1) the SEQ ID NO: 2 or SEQ ID NO: 4 DNA sequence in sequence table; 2) coding the DNA sequence of SEQ ID NO: 1 in the sequence table; 3) the nucleotide sequence that can hybrid with restrict DNA sequence of SEQ ID NO: 2 in the sequence table at high strict condition. The gene and its protein have important theory and operation significance in studying the molecular mechanism of rice tillering angulations and controlling rice plant type. The invention has extensive application and market outlook in agriculture field.

Description

Genes regulating rice tiller angle and their encoded proteins and their application

technical field

The invention relates to a plant gene and its coded protein and its application, in particular to a gene for regulating tillering angle of rice and its coded protein and its application in regulating the tillering angle of rice.

Background technique

The plant type of rice controls the three-dimensional distribution of its different tissues on the plant. It can properly allocate and adjust the space, light, temperature, water, and humidity of the paddy field to promote the normal development of the plant. The plant type of rice includes tiller angle, phyllotaxy, panicle type and plant height, among which tiller angle is an important factor affecting plant type. Tiller angle refers to the angle between the tiller and the main stem and other tillers (Xu Y B, McCouch S R, Shen Z T. Transgressive segregation of tiller angle in rice caused by complementary gene action. CropSci, 1998, 38: 12～19 .), too large a tiller angle not only wastes space, reduces the rice planting density per unit area and leads to a decrease in yield, but also is not conducive to the mechanized harvesting of rice; on the contrary, too small a tiller angle will make the growth of the plant too cramped, ventilated and light-transmitting Unsmooth, leading to vicious competition between tillers, the photosynthetic efficiency of the group is reduced, and the humidity is high and it is easy to be infected. Therefore, a reasonable tillering angle is conducive to adjusting the density of rice populations, improving the photosynthetic efficiency of the population, promoting rice growth and increasing yield. At present, improving the tiller angle of rice is still a time-consuming and labor-intensive task for breeders, and the direct transfer of genes that control the tiller angle into rice can greatly improve the efficiency of this work.

Studies have shown that tiller angle in rice is regulated by multiple genes. Takahashi found that these two phenotypes were controlled by a pair of recessive genes la and er(o) respectively after studying the scattered mutants with large tiller angles and the mutants with upright growth in rice (Takahashi M. Linkage groups and gene schemes of somestriking morphological characters in Japanese rice. Symp Rice Genet Cytogenet Internal Rice Inst (1963), Elsevier Amsterdam, 1963, 215-236.); Abenes, Abe and Li Peijin respectively located the la gene on the 11th chromosome of rice, and thought that Rice sporadic mutants are associated with negative gravitropism of plants; negative gravitropism means that plants will grow against the direction of gravity during growth; rice la mutant tillers are not sensitive to gravity, and it will form a Large angle and flat (Abenes MLP, Tabien RE, McCouch SR, Ikeda R, Ronad P, Khush GS, Huang N. Orientation and integration of the classical and molecular genetic maps of chromosome 11 in rice. Euphytica, 1994, 76: 81～ 87.; Abe K, Takahashi H, Suge H. Lazy gene(la) responsible for both an agravitropism of seeding and lazy habit of tillergrowth in rice(Oryza.sativa L.). J Plant Res, 1996, 109(1096): 381～386.; Li Peijin, Zeng Dali, Liu Xinfang, Xu Dan, Gu Dai, Li Jiayang, Qian Qian. Genetics and gene mapping of rice sporadic mutants. Science Bulletin, 2003, 48(21): 2271～2274.) . The plant types of indica rice and japonica rice are quite different. Generally, the tiller angle of indica rice is larger than that of japonica rice. Through the establishment of high-generation backcross populations between indica and japonica rice, multiple QTLs have been mapped in the DH population and recombinant inbred lines, and the main It is possible to decompose effective QTLs into individual Mendelian factors. Yamamoto et al. identified a dominant single gene Spk(t) from indica rice through the indica-japonica backcross population, which controls the loose plant type (Yamamoto T, Sasaki T, Yano M. Genetic analysis of spreading stubusing ind-ica/japonica backcrossed progenies in rice. Breeding Sci, 1997, 47: 141-144.); Li et al. used the Lemont/Teqing indica-japonica cross F2: 4 segregation population to also locate the main gene Ta that controls tiller angle on chromosome 9, and located 4 QTLs (QTa1, QTa2, QTa5 and QTa8) (Li Z, Paterson AH, Pinson SRM, Khush GS.A major gene, Tall, and QTLs affecting tiller and leaf angles. Rice Genet Newsl, 1998, 15:154～159. ); Qianqian et al. detected three QTLs controlling tiller angle (qTA-9a, qTA-9b and qTA-12) (Qian Qian, He Ping, Teng Sheng, Zeng Dali, Zhu Lihuang. QTL Analysis of Rice Tiller Angle. Acta Genetica Sinica, 2001, 28(1): 29-32.). Shen Shengquan et al. used the recombinant inbred line constructed by Xieqingzao B/Miyang 46 to locate two QTLs (qTA8-2 and qTA9-2) with significant additive effects (Shen Shengquan, Zhuang Jieyun, Bao Jinsong, Zheng Kangle, Xia Yingwu, Shu Qingyao. QTL Analysis of Rice Tiller Maximum Angle. Journal of Agricultural Biotechnology, 2005, 13(1): 16～20.).

Contents of the invention

The purpose of the present invention is to provide a gene regulating rice tiller angle.

The gene regulating the tiller angle of rice provided by the present invention is called compact1 (abbreviated as com1), which is derived from Oryza rice (Oryza sativa L.), and its cDNA is one of the following nucleotide sequences:

1) The DNA sequence of SEQ ID №: 2 or SEQ ID №: 4 in the sequence listing;

2) The DNA sequence of SEQ ID №: 1 in the coding sequence list;

3) A nucleotide sequence that can hybridize to the DNA sequence defined by SEQ ID №: 2 or SEQ ID №: 4 in the sequence listing under high stringency conditions.

The high stringency condition is to hybridize and wash the membrane at 65° C. in a solution of 0.1×SSPE (or 0.1×SSC) and 0.1% SDS.

SEQ ID №: 2 in the sequence listing consists of 948 bases, and its coding sequence is the 21st-800th base from the 5' end, encoding a protein with the amino acid residue sequence of SEQ ID №: 1 in the sequence listing, Bases 210-281 from the 5' end encode the ZNF-NFX zinc finger protein domain; SEQ ID No. 4 in the sequence listing consists of 2637 bases, and bases 832-2382 from the 5' end are its The fourth intron sequence of genomic DNA.

Its genome gene is one of the following nucleotide sequences:

1) The DNA sequence of SEQ ID №: 3 in the sequence listing;

2) A nucleotide sequence that can hybridize to the DNA sequence defined by SEQ ID No. 3 in the sequence listing under high stringency conditions.

The high stringency condition is to hybridize and wash the membrane at 65° C. in a solution of 0.1×SSPE (or 0.1×SSC) and 0.1% SDS.

SEQ ID № in the sequence listing: 3 consists of 3137 bases, bases 1-57 from the 5' end are the first exon of the genome gene, bases 58-224 from the 5' end It is the first intron of the genome gene, and the 225-291 base from the 5' end is the second exon of the genome gene, and the 292-394 base from the 5' end is the genome gene The second intron from the 395th-1011th base of the 5' end is the third exon of the genome gene, and the 1012th-1241th base from the 5' end is the third exon of the genome gene Intron, the 1242-1331 base from the 5' end is the fourth exon of the genome gene, and the 1332-2883 base from the 5' end is the fourth intron of the genome gene, Bases 2884-3137 from the 5' end are the fifth exon of the genome gene, bases 46-48 from the 5' end are the start codon ATG of the genome gene, bases from the 5' end Bases 1323-1325 are the stop codon TAA of the genome gene, bases 505-576 from the 5' end encode the ZNF-NFX zinc finger protein domain; bases 2882 from the 5' end are control rice strains When the base is A, the rice is a dispersed plant type with a large tiller angle; when the base is G, the rice is a compact plant type with a small tiller angle.

The protein (compact1, com1 for short) encoded by the gene regulating rice tiller angle of the present invention has one of the following amino acid residue sequences:

1) SEQ ID №: 1 in the sequence listing;

2) The amino acid residue sequence of SEQ ID №: 1 in the sequence table is substituted, deleted or added with one to ten amino acid residues, and has the function of regulating rice tiller angle.

SEQ ID №1 in the sequence listing consists of 259 amino acid residues, and amino acid residues 64-87 from the amino terminal (N-terminal) are ZNF-NFX zinc finger protein domains.

The expression vector, transgenic cell line and host bacteria containing the gene of the present invention all belong to the protection scope of the present invention.

The primer pair for amplifying any fragment in com1 is also within the protection scope of the present invention.

Another object of the present invention is to provide a method for regulating rice tiller angle.

The method for regulating tillering angle of rice provided by the present invention is to introduce the gene com1 regulating tillering angle of rice into rice tissues or cells, and the tillering angle of rice is regulated.

The gene com1 that regulates the rice tiller angle can be introduced into explants through a plant expression vector containing com1; the starting vector for constructing the plant expression vector can be any binary Agrobacterium vector or can be used for plant microprojectile bombardment vectors, etc., such as pCAMBIA1301-UbiN (GenBank No.: AF234296), pBI121, pBin19, pCAMBIA2301, pCAMBIA1300 or other derived plant expression vectors.

When com1 is used to construct a plant expression vector, any enhanced, constitutive, tissue-specific or inducible promoter can be added before its transcription initiation nucleotide, such as the cauliflower mosaic virus (CAMV) 35S promoter, Ubiquitin gene Ubiquitin promoter (pUbi) and rice actin gene promoter (Actin), etc., they can be used alone or in combination with other plant promoters; in addition, when using the gene of the present invention to construct a plant expression vector, Enhancers can also be used, including translation enhancers or transcription enhancers, and these enhancer regions can be ATG start codons or adjacent region start codons, etc., but must be the same as the reading frame of the coding sequence to ensure the integrity of the entire sequence. translate correctly. The sources of the translation control signals and initiation codons are extensive and can be natural or synthetic. The translation initiation region can be from a transcription initiation region or a structural gene.

In order to facilitate the identification and screening of transgenic plant cells or plants, the plant expression vectors used can be processed, such as adding genes (GUS genes, GFP genes, fluorescent luciferase gene, etc.), antibiotic markers with resistance (gentamycin marker, kanamycin marker, etc.) or chemical resistance marker genes (such as herbicide resistance genes), etc. Considering the safety of the transgenic plants, the transformed plants can be screened directly by adversity without adding any selectable marker gene.

Using pCAMBIA1301-UbiN as the starting vector, the constructed plant expression vector is pCambia1301-UbiN-COM1.

The plant expression vector carrying the rice tiller angle regulating gene com1 of the present invention can transform rice cells or tissues by conventional biological methods such as Ti plasmid, Ri plasmid, plant virus vector, direct DNA transformation, microinjection, conduction, Agrobacterium mediation, etc. and cultivating transformed rice cells or tissues into plants.

The invention provides a gene for regulating rice tiller angle and its encoded protein. The gene has important theoretical and practical significance for the study of the molecular mechanism of rice tiller angle and the regulation of rice plant type, and provides an economical, fast and effective way to improve the plant type of crops. The invention has broad application and market prospects in the agricultural field.

The present invention will be further described below in conjunction with specific embodiments.

Description of drawings

Figure 1 is a comparison of the compact line IL55 and the loose line IR24

Figure 2A is the distribution of Asominory introgression fragments on all chromosomes

Figure 2B is the fine mapping map of Asominory introgression fragments on chromosome 9

Figure 3A is the localization map of com1 and RM201, CP8, CP3, CP5 and RM1026

Figure 3B is a schematic diagram of the localization map of the 8 SSPL markers developed between RM201 and CP8 and the distribution of candidate com1

Figure 3C is the agarose gel electrophoresis detection results of the cDNAs of 6 candidate com1 in IL55 and IR24

Figure 4 is a schematic diagram of the splicing of the fourth intron in IL55 and IR24

Figure 5 shows the results of agarose gel electrophoresis detection of cDNA of LOC_Os09g35980 in rice with different scattered plant types and compact plant types

Figure 6 is a partial physical map of the pCambia1301-UbiN-COM1 plant expression vector

Figure 7 shows com1 transgenic plants with scattered plant types

Figure 8 is the PCR detection result of com1 transgenic plants

Detailed ways

The methods used in the following examples are conventional methods unless otherwise specified, and the primer synthesis and sequencing work are all completed by Shanghai Sangon Bioengineering Co., Ltd.

Example 1. Acquisition of transcription factor gene compact1 regulating rice tiller angle

Rice varieties: Asominori, IR24, Dongxiang wild rice, Yuanjiang wild rice, Teqing, 9311, Guichao 2, E32, Nipponbare, Qiuguang, Shuiyuan 349 (all from the rice group seed bank of the College of Agronomy and Biotechnology, China Agricultural University) .

Using the japonica rice Asominori as the donor and the indica rice IR24 as the recipient, a high-generation backcross population (BC3F4) was constructed, from which a compact line with a small tiller angle was found. The line IL55 with differences in IR24 (see Figure 1 for the comparison of the compact line IL55 and the loose line IR24) was crossed with IR24 to construct a F2 segregation population containing 12,000 individuals. In this F2 population, the ratio of scattered individuals to compact individuals was 9104:2896, in line with the ratio of 3:1, indicating that the gene controlling plant type is a recessive gene, named compact1 (com1). In order to isolate the com1 gene, the genotypes of the two lines IL55 and IL65 with the same compact plant type and other phenotypes were compared first, and it was found that the two only had a common Asominori introgression segment on chromosome 9 (the Asominori introgression segment was in The distribution map on all chromosomes is shown in Figure 2A, and the location map of Asominori infiltrated fragments on chromosome 9 is shown in Figure 2B, indicating that com1 may be located on chromosome 9, which is consistent with previous studies that there may be a The results of the main QTL were consistent (Yamamoto T, Sasaki T, Yano M. Genetic analysis of spreading stubusing indica/japonica backcrossed progenies in rice. Breeding Sci, 1997, 47: 141-144.; Li Peijin, Zeng Dali, Liu Xinfang, Xu Dan, Gu Dai, Li Jiayang, Qian Qian. Inheritance and gene mapping of rice sporadic mutants. Science Bulletin, 2003, 48(21): 2271-2274.; Qian Qian, He Ping, Teng Sheng, Zeng Dali, Zhu Lihuang. Rice tillering QTL analysis of angle. Acta Genetics, 2001, 28(1): 29～32.; Shen Shengquan, Zhuang Jieyun, Bao Jinsong, Zheng Kangle, Xia Yingwu, Shu Qingyao. QTL analysis of rice tillering maximum angle. Journal of Agricultural Biotechnology, 2005, 13(1): 16～20.). Linkage analysis of 400 compact individuals using SSR markers located on the Asominory introgression segment of chromosome 9 revealed that RM201 and RM1026 are closely linked to the com1 gene and will The com1 gene is stuck in this section, and then according to the online genome sequence in this section, three Simple Sequence Length Polymorphism (SSLP) markers CP3, CP5, and CP8 were developed to initially locate the com1 gene between RM201 and CP8 On the segment of com1, the distances between com1 and these two markers are 4.0cM and 2.4cM respectively (see Figure 3A). Then, 2496 compact recessive individual plants were used to fine-map com1, according to the distance between RM201 and CP8 Eight SSPL markers were successively developed for the genome sequence (see Figure 3B), and com1 was finally compressed in the range of 35Kb between P4 and P6, and P5 was co-segregated with com1. Within this 35Kb range, according to the TIGR rice genome annotation ( HTTP://WWW.TIGR.ORG) found 6 genes, namely: LOC_Os09g35980, LOC_Os09g35990, LOC_Os09g36000, LOC_Os09g36010, LOC_Os09g36020, LOC_Os09g36030 (see Figure 3B). Compare IR24 and IL655 genes in the cDNA The detection results of 1% agarose gel electrophoresis are shown in Figure 3C (lane M is a Marker in the range of 1Kb-3Kb), and the detection results show that there is a huge difference between the two cDNAs of LOC_Os09g35980, the former is about 1kb, and the latter is about 2.5Kb. To find out the reason for this large discrepancy, the genomic and cDNA sequences of the LOC_Os09g35980 gene in IR24 and IL55 were compared, respectively. The genomic DNA of LOC_Os09g35980 (corresponding to the full-length cDNA of AK066042) consists of five exons and four introns, has the nucleotide sequence of SEQ ID №: 3 in the sequence listing, and SEQ ID №: 3 in the sequence listing Consisting of 3137 bases, bases 1-57 from the 5' end are the first exon of the genome gene, and bases 58-224 from the 5' end are the first intron of the genome gene Intron, bases 225-291 from the 5' end are the second exon of the genome gene, bases 292-394 from the 5' end are the second intron of the genome gene, since Bases 395-1011 at the 5' end are the third exon of the genome gene, bases 1012-1241 at the 5' end are the third intron of the genome gene, bases at the 5' end are the third intron Bases 1242-1331 are the fourth exon of the genome gene, bases 1332-2883 from the 5' end are the fourth intron of the genome gene, bases from the 5' end 2884-3137 The base is the fifth exon of the genome gene, the 46th-48th base from the 5' end is the start codon ATG of the genome gene, and the 1323-1325th base from the 5' end is the genome The stop codon TAA of the gene encodes the ZNF-NFX zinc finger protein domain from the 505th-576th base at the 5' end. The above two cDNA fragments were recovered and sequenced. The sequencing results showed that the cDNA of LOC_Os09g35980 in IR24 had the nucleotide sequence of SEQ ID №: 2 in the sequence listing, and the SEQ ID №: 2 in the sequence listing consisted of 948 bases , its coding sequence is the 21st-800th base from the 5' end, which encodes a protein with the amino acid residue sequence of SEQ ID №1 in the sequence listing, and the 210th-281st base from the 5' end encodes ZNF-NFX Zinc finger protein domain; the cDNA of LOC_Os09g35980 in IL55 has the nucleotide sequence of SEQ ID №: 4 in the sequence listing, and the SEQ ID №: 4 in the sequence listing consists of 2637 bases, from 5′ end 832-2382 The base is the fourth intron sequence of LOC_Os09g35980 genomic DNA. In IR24, the four introns of LOC_Os09g35980 genomic DNA could be spliced normally, but in IL55, only the first three introns could be spliced normally, and the fourth intron could not be spliced. The genomic DNA of LOC_Os09g35980 in IR24 has the nucleotide sequence of SEQ ID №: 5 in the sequence table, the genomic DNA of LOC_Os09g35980 in IL55 has the nucleotide sequence of SEQ ID №: 6 in the sequence table, and the genomic DNA sequence of LOC_Os09g35980 in both There are 12 differences: there is a single nucleotide (SNP) difference in the third exon (ie, the 996th base from the 5' end in SEQ ID №: 3), and two in the fifth exon. A SNP difference (that is, respectively located at the 2984th and 2988th bases from the 5' end in SEQ ID №: 3), a 4bp deletion fragment (that is, the 68th-71st positions from the 5' end in SEQ ID №: 3 base) and two SNPs (that is, respectively located at the 381st and 1083rd bases from the 5' end in SEQ ID №3) are respectively located in the first three introns, and exist in the fourth intron 6 SNP differences (that is, respectively located at 1409, 2382, 2387, 2653, 2686, and 2879 bases from the 5′ end of SEQ ID №: 3) and a 1bp deletion fragment (that is, SEQ ID №: 2768th base from the 5' end of 3), among the 6 SNP differences in the fourth intron, one of the SNPs is at the 3' end splice site of the fourth intron (AG(IR24) → GG (IL55), that is, on the 2882-2883 bases from the 5' end in SEQ ID №: 3), this makes the fourth intron in IL55 unable to be spliced (see Figure 4, in Figure 4 Figure A is a schematic diagram of the structure of introns and exons of IR24 and IL55, and Figure B is a schematic diagram of the splicing of IR24 and IL55 introns), which are consistent with the above sequencing results. Surprisingly, the stop codon is at the 3' end of the fourth exon (the 1323-1325th base from the 5' end in SEQ ID №3 is the stop codon TAA of the genome gene), Since the first three introns of this gene can be spliced normally in IR24 and IL55, the translatable cDNA of this gene in these two lines is of equal length. In order to verify whether LOC_Os09g35980 is the com1 gene, and confirm whether the SNP difference located in the third exon or the splicing failure of the fourth intron caused the phenotypic differences of IR24 and IL55, Dongxiang wild rice with scattered plant types ( DXCWR) and Yuanjiang wild rice (YJCWR), four indica rice Teqing (TQ), 9311, Guichao 2 (GC-2) and E32; three compact japonica rice Nipponbare (NP), Qiuguang (QG) and Shuiyuan 349 (sewon349), compared the genomic DNA and cDNA sequences of LOC_Os09g35980 among them, the detection results of the cDNA sequences are shown in Figure 5 (s indicates a scattered plant type, c indicates a compact plant type), and found that Dongxiang wild rice with scattered plant types, The cDNA of LOC_Os09g35980 of Yuanjiang wild rice, Teqing, 9311, Guichao 2, E32 is the same length as that of IR24 (about 1 kb), while the cDNA of LOC_Os09g35980 of compact plant type Nipponbare, Qiuguang and Suwon 349 is the same length as that of IL55 ( About 2.5Kb), it proves that only the SNP at the splicing site of the fourth intron (base 2882 from the 5′ end in SEQ ID №3) is closely linked to its phenotype, that is, LOC_Os09g35980 of the scattered plant type with large tiller angle The base of the splicing site of the fourth intron is AG, and the base of the splicing site of the fourth intron of LOC_Os09g35980, which is a compact plant with a small tiller angle, is GG (see Figure 5). Next, 6 indica plants with scattered plant type and 16 japonica plants with compact plant type were selected, and the same method as above was used to further verify that the SNP at the splicing site of the fourth intron was closely linked to the plant type. The above analysis results show that LOC_Os09g35980 is the com1 gene, and the protein encoded by this gene is named compact1 (abbreviated as com1). Composed of 259 amino acid residues, amino acid residues 64-87 from the amino terminal (N-terminal) are ZNF-NFX zinc finger protein domains.

Example 2, the acquisition of com1 transgenic rice and its PCR detection

1. Construction of com1 plant expression vector

Primers were designed according to the full-length cDNA sequence of com1 of IR24, and restriction endonuclease Sma I and Spe I recognition sites and protective bases were respectively introduced at both ends of the primers. The primer sequences are as follows:

CDS-P-ATG: 5'- TGCCCCGGG CGTACTGTCTGGCTTTCTCTTCTGGT-3' (underlined bases are restriction endonuclease Sma I recognition sites and protected bases);

CDS-P-TGA: 5'- CACTAGT AGGACTATTCTTCATCACTGGCG-3' (underlined bases are restriction endonuclease Spe I recognition sites and protected bases)

Use TRIZOL reagent to extract the RNA of the leaves of IR24 seedling stage (three leaves and one heart), use this RNA as a template, use MMLV reverse transcriptase to perform reverse transcription to obtain cDNA, and then use this cDNA as a template, in the primer CDS-P-ATG Under the guidance of the primer CDS-P-TGA, the CDS sequence of rice com1 was amplified by the conventional PCR method. After the reaction, the PCR amplification product was detected by 1% agarose gel electrophoresis, and a 948bp DNA fragment was recovered and purified. It is cloned into the plant expression vector pCambia1301-UbiN (GenBank No.: AF234297) between the SmaI and SpeI restriction sites of the multiple cloning site to obtain the plant expression vector of rice com1, which is called pCambia1301-UbiN-COM1. Part of the physical map is shown in Figure 6.

2. Transformation of rice

The plant expression vector pCambia1301-UbiN-COM1 of the rice com1 constructed in step 1 was transformed into the mature embryo callus of the compact Nipponbare by the particle gun method, and the NB medium containing 50 mg/L hygromycin [N ₆ macroelements ( Zhu Zhiqing et al. 1974) Wang JJ (Wang Jingju), Sun JS (Sun Jingsan), Zhu ZQ (Zhu Zhiqing) On the conditions for the induction of rice pollen plantlets and certain factors affecting the frequency of induction. Acta Bot Sin (Journal of Botany), 1974, 16 : 43-54 (in Chinese); B5 trace elements, organic components and iron salts (Gamborg, 1968) Gamborg OL, MilerR A, Ojima K.Nutrient requirement of suspension cultures of soybean rootcells[J].Exp Cell Res, 1968, 50:151-158.] Two rounds of screening were carried out, and each round was selected for 20-30 days, and then pre-differentiated and differentiated to obtain transgenic plants with dispersed plant types, as shown in FIG. 7 .

3. PCR Identification of Transgenic Rice

Genomic DNA of transgenic rice with scattered plant types was extracted and used as a template for PCR detection under the guidance of primer 1: 5'-GTACTGTCTGGCTTTCTCTTCTGGT-3' and primer 2: 5'-AGGACTATTCTTCATCACTGGCG-3' to transform the empty vector The plants of pCambia1301-UbiN were used as the control, and the PCR reaction conditions were as follows: 94°C for 3min; then 94°C for 30sec, 60°C for 30sec, 72°C for 1min30sec, a total of 30 cycles; finally 72°C for 10min. After the reaction, the amplified products were detected by 1% agarose gel electrophoresis, and the detection results were as shown in Figure 8 (swimming lane 1: positive control, swimming lane 2: transformed plants with empty vector pCambiai301-UbiN, swimming lanes 3-9: Positive transgenic plants transformed with pCambia1301-UbiN-COM1, lane M: Marker), the positive com1 transgenic plants can amplify a band of about 1Kb.

After phenotypic and PCR identification, 7 positive T0 _generation com1 transgenic plants were finally obtained. The plant types were scattered and the characters were complemented, indicating that the com1 of the present invention can be used to regulate rice plant type.

sequence listing

<160>6

<210>1

<211>259

<212>PRT

<213>Oryza sativa L. indica

<400>1

Met Ala Leu Lys Val Phe Asn Trp Leu Asn Arg Lys Lys His Ser Asn

1 5 10 15

Val Glu Tyr Cys Thr Ile Asn Glu Asn Lys Ala Met Glu Glu Lys Glu

20 25 30

Asp Ser Leu Arg Ala Ser Val Thr Glu Gln Asp Thr Glu Ala Leu Leu

35 40 45

Leu Arg Asp Val Leu Ile Asn Gly Ile Leu Ala Ile Gly Thr Leu Gly

50 55 60

His Asn Val Asn Ser Leu Cys Pro Glu Ser Cys Ile Glu Gln Asp Glu

65 70 75 80

Pro Ile Ile Met Cys Asp Glu Lys Val Glu Gln Glu Lys Cys Glu Glu

85 90 95

Glu Lys Ala Glu Ala Lys Gln Asp Thr Pro Val Thr Ala Pro Ser Glu

100 105 110

Pro Ala Ser Ala Leu Glu Pro Ala Lys Met His Ser Ser Ser Met Lys

115 120 125

Glu Asp Asn Phe Met Cys Phe Val Lys Glu Glu Ile Leu Met His Gly

130 135 140

Met Glu Val Glu Asp Val Pro Asn Ile Gln Glu Arg Pro Leu Leu Met

145 150 155 160

Leu Glu Lys Val Glu Lys Val Arg Thr Thr Leu Ala Asp Leu Phe Ala

165 170 175

Ala Glu Ala Phe Ser Ser Ser Ser Asp Ala Glu Asp Lys Cys Tyr Pro Lys

180 185 190

Ile Val Ile Val Ala Gly Ala Ser Thr Ser Lys Pro Thr Ser Cys Met

195 200 205

Glu Lys Met His His His Lys Lys Pro Thr Lys Pro Thr Ser Lys Pro Leu

210 215 220

Lys Ala Thr Arg Lys Leu Ser Arg Val Met Arg Lys Met Leu Gly Lys

225 230 235 240

Lys Ile His Pro Glu Gln Leu Asn Gly Arg Ser Asn Ala Glu Gly Pro

245 250 255

Val Thr Ala

<210>2

<211>948

<212> cDNA

<213>Oryza sativa L. indica

<400>2

ttcatattgg ttctagagag atggctctaa aggtgttcaa ttggctgaat cggaagaagc 60

attctaatgt cgagtattgc accatcaatg agaacaaggc catggaagag aaggaagact 120

ctctgcgtgc aagtgtgact gagcaagaca ctgaggccct gctgctccgt gatgtgctta 180

ttaatggtat acttgcaatt ggcacgctgg gccacaatgt aaactcactc tgtcctgagt 240

cctgtattga acaagatgag cccatcatca tgtgtgatga gaaagtggaa caagagaagt 300

gcgaagaaga aaaggctgag gctaaacagg acacaccagt tacagcacca agtgaaccgg 360

catctgctct tgagcctgcc aagatgcact catcatcgat gaaagaagac aacttcatgt 420

gctttgtgaa ggaggaaatc ctaatgcatg gcatggaagt ggaagatgtt cctaacatcc 480

aggaacgacc acttctgatg ttagagaagg tggagaaagt gagaactaca cttgccgatc 540

tatttgctgc agaagcattc tcatcaagtg atgcagagga taagtgttac ccgaaaatcg 600

tcattgttgc tggggcatcc acttcaaagc ctacgtcgtg catggagaag atgcatcaca 660

agaagccaac aaaaccaacg tcaaagccgc tgaaggctac gagaaaatta agtcgagtca 720

tgaggaagat gttggggaag aagatccacc cagagcagct caatggacgt agcaatgcag 780

agggccctgt cactgcataa tgctaggatt tgttggatcc atcctcacgc ttcggattcc 840

ttgctcaaga gaaacatcca tgcatatcgt cgacagcgtt cgttcagtcc tcttcctttt 900

gttgttgttg ctgttgttat tattgttatt gttattgctg cctattcg 948

<210>3

<211>3137

<212>DNA

<213>Oryza sativa

<220>

<221>misc-feature

<222>(2882)

<223>n=a or g

<400>3

gtactgtctg gctttctctt ctggtttcat attggttcta gagagatggc tctaaaggta 60

cacagttagt tcatgctgat acaccatttg tccgtgtata gtgtatgctg taaagtacag 120

gtctttgcag tttctgtctc tcttaaacaa gtgcgttgga ttcaactcac tgttcttata 180

tctaatgaga ttactggtga tttatgtttt tatggcacta ccaggtgttc aattggctga 240

atcggaagaa gcattctaat gtcgagtatt gcaccatcaa tgagaacaag ggtaaggata 300

ccaggatatc tctcttttat gtaccacatg ttttgttgtt ctcatgttgt caactcttgt 360

ttcttctctt ctattttttt tcctgatgga gtagccatgg aagagaagga agactctctg 420

cgtgcaagtg tgactgagca agacactgag gccctgctgc tccgtgatgt gcttattaat 480

ggtatacttg caattggcac gctgggccac aatgtaaact cactctgtcc tgagtcctgt 540

attgaacaag atgagcccat catcatgtgt gatgagaaag tggaacaaga gaagtgcgaa 600

gaagaaaagg ctgaggctaa acaggacaca ccagttacag caccaagtga accggcatct 660

gctcttgagc ctgccaagat gcactcatca tcgatgaaag aagacaactt catgtgcttt 720

gtgaaggagg aaatcctaat gcatggcatg gaagtggaag atgttcctaa catccaggaa 780

cgaccacttc tgatgttaga gaaggtggag aaagtgagaa ctacacttgc cgatctattt 840

gctgcagaag cattctcatc aagtgatgca gaggataagt gttacccgaa aatcgtcatt 900

gttgctgggg catccacttc aaagcctacg tcgtgcatgg agaagatgca tcacaagaag 960

ccaacaaaac caacgtcaaa gccgctgaag gctacaagaa aattaagtcg agtatggttt 1020

tcttcgtctc tgctttatt gttaagcctt gttacattta attttactag tctggaattt 1080

attacacttt gttccatggt cagtgcgcca catcttacac atatgtttta ccatatagct 1140

acaactggag taagcattat gcatctagcc agtctttagt ggtaaatgat cactgacctg 1200

gaatgcacct ttttggtttt tgtcatctgt aaaataagta ggtcatgagg aagatgttgg 1260

ggaagaagat ccaccccagag cagctcaatg gacgtagcaa tgcagagggc cctgtcactg 1320

cataatgcta ggtttgtgga caaaagtttc ctttctctat ctggcaattt attacctaga 1380

gtttttttaa agctgtctgt tgaactatac agggctcaag gcccttgtgt tttgcatacg 1440

attttgtacc tttccaaatt ttcttgattt ttacttggat tccatgttt gtaataaaat 1500

taggttaata tctgaggtag tatttgcata gaatggatac cttctaccaa agttatattt 1560

gtttgtgggt cctagagcta gcatgaccca cgaatcacat tcaagaatat agtaattcgt 1620

ttaggctaca tttcactagg aatagaacat atgaatattc tcaggtttaa taaggcagat 1680

gcaaaaaaga actgaatagc acaggaaagt aatttttcca tttcaagctt ctcacagtca 1740

aaaacaagta atggttcaaa aattgaatat tctatcacct gttgcctcca tttatgtgga 1800

actcacaaga gggtctaagt gctgcttgac actctgacct gtatttaaat aaaatgttat 1860

cacctattgc ctctgcttat tattgggatc tcaaaacaca atctattact gtgtatgcct 1920

tggcattttt taaaatctga gctgccccct tattcttcac attttctcag aaaaccatat 1980

aaacttttag ataaatgaag cttttattga tctcagacaa ttacatcaag ttgatagaac 2040

caaactaaga acacttctgg cctctgataa tggaactgct gtttgtttaa gtagaaagaa 2100

tggtgtgtgt attttaacgg ttggtgattg ggaattggga tgcagtgacc atggagactg 2160

tgttcttcag aatattttta agaaatagtg ttttcggtgg atcctatcat ggaactgcct 2220

atggtggaaa gagcaatacg agttgaaaca tatagggatt aggaagcatg ggttcggact 2280

aaaatgcatt cgttcctgcc atgatgcaaa gcttcattcg aagcaaaatg agcacatgcc 2340

atggtacagt ggcgtgcaac gagcactgca attgcacacg acgtgcatgt tctgaaaaag 2400

gccagtggac actagacatg cagctaatcc tcttttgatg aattctatca tgaaaccaat 2460

ggattcgttg aaaatctgaa caaattgatg gatttggtcc cagctgtaga aaggttggca 2520

aactcttctc gctcctgtat tattgtaggc tggagctatg atggaccaaa tcatcagaag 2580

caaatttcac catatataag catctacttt tatctttttc tctattttaa aatgtgatca 2640

tagtgaagtt atatacattc tatctgctta acgctccaca ttatccaaaa aaatgcatca 2700

cactccatat tgggaacacg ttggtggttc tgaaccaaag tcgatctctt gatgccaatt 2760

ttttttttga gctggttgag tagtcgaact gggaacaatt actgcttgag gctacaaaat 2820

ttctggccga caatacgtct tgatcaggaa ctgactaaag aaattccctt tcaccttttg 2880

cnggatttgt tggatccatc ctcacgcttc ggattccttg ctcaagagaa acatccatgc 2940

atatcgtcga cagcgttcgt tcagtcctct tccttttgtt gttggtgctg ttgttattat 3000

tgttattgtt attgctgcct attcgctcgc cagtgatgaa gaatagtcct gcctatattt 3060

gcctgtagta cattgtaaag cctacagttga cgtgtcttgt aagaccctta ttatattgt 3120

ccataccacg acgtctc 3137

<210>4

<211>2637

<212> cDNA

<213>Oryza sativa L.japonica

<400>4

gtactgtctg gctttctctt ctggtttcat attggttcta gagagatggc tctaaaggtg 60

ttcaattggc tgaatcggaa gaagcattct aatgtcgagt attgcaccat caatgagaac 120

aaggccatgg aagagaagga agactctctg cgtgcaagtg tgactgagca agacactgag 180

gccctgctgc tccgtgatgt gcttattaat ggtatacttg caattggcac gctgggccac 240

aatgtaaact cactctgtcc tgagtcctgt attgaacaag atgagcccat catcatgtgt 300

gatgagaaag tggaacaaga gaagtgcgaa gaagaaaagg ctgaggctaa acaggacaca 360

ccagttacag caccaagtga accggcatct gctcttgagc ctgccaagat gcactcatca 420

tcgatgaaag aagacaactt catgtgcttt gtgaaggagg aaatcctaat gcatggcatg 480

gaagtggaag atgttcctaa catccaggaa cgaccacttc tgatgttaga gaaggtggag 540

aaagtgagaa ctacacttgc cgatctattt gctgcagaag cattctcatc aagtgatgca 600

gaggataagt gttacccgaa aatcgtcatt gttgctgggg catccacttc aaagcctacg 660

tcgtgcatgg agaagatgca tcacaagaag ccaacaaaac caacgtcaaa gccgctgaag 720

gctacaagaa aattaagtcg agtcatgagg aagatgttgg ggaagaagat ccaccccagag 780

cagctcaatg gacgtagcaa tgcagagggc cctgtcactg cataatgcta ggtttgtgga 840

caaaagtttc ctttctctat ctggcaattt attacctaga gtttttttaa agctgtctgt 900

tgaactatac agggctcaag gcccttgtgt tttgcatacg attttgtacc tttccaaatt 960

ttcttgattt ttacttggat tccattgttt gtaataaaat taggttaata tctgaggtag 1020

tatttgcata gaatggatac cttctaccaa agttatattt gtttgtgggt cctagagcta 1080

gcatgaccca cgaatcacat tcaagaatat agtaattcgt ttaggctaca tttcactagg 1140

aatagaacat atgaatattc tcaggtttaa taaggcagat gcaaaaaaga actgaatagc 1200

acaggaaagt aatttttcca tttcaagctt ctcacagtca aaaacaagta atggttcaaa 1260

aattgaatat tctatcacct gttgcctcca tttatgtgga actcacaaga gggtctaagt 1320

gctgcttgac actctgacct gtatttaaat aaaatgttat cacctattgc ctctgcttat 1380

tattgggatc tcaaaacaca atctattact gtgtatgcct tggcattttt taaaatctga 1440

gctgccccct tattcttcac attttctcag aaaaccatat aaacttttag ataaatgaag 1500

cttttattga tctcagacaa ttacatcaag ttgatagaac caaactaaga acacttctgg 1560

cctctgataa tggaactgct gtttgtttaa gtagaaagaa tggtgtgtgt attttaacgg 1620

ttggtgattg ggaattggga tgcagtgacc atggagactg tgttcttcag aatattttta 1680

agaaatagtg ttttcggtgg atcctatcat ggaactgcct atggtggaaa gagcaatacg 1740

agttgaaaca tatagggatt aggaagcatg ggttcggact aaaatgcatt cgttcctgcc 1800

atgatgcaaa gcttcattcg aagcaaaatg agcacatgcc atggtacagt ggcgtgcaac 1860

gagcactgca attgcacacg acgtgcatgt tctgaaaaag gccagtggac actagacatg 1920

cagctaatcc tcttttgatg aattctatca tgaaaccaat ggattcgttg aaaatctgaa 1980

caaattgatg gatttggtcc cagctgtaga aaggttggca aactcttctc gctcctgtat 2040

tattgtaggc tggagctatg atggaccaaa tcatcagaag caaatttcac catatataag 2100

catctacttt tatctttttc tctattttaa aatgtgatca tagtgaagtt atatacattc 2160

tatctgctta acgctccaca ttatccaaaa aaatgcatca cactccatat tgggaacacg 2220

ttggtggttc tgaaccaaag tcgatctctt gatgccaatt ttttttttga gctggttgag 2280

tagtcgaact gggaacaatt actgcttgag gctacaaaat ttctggccga caatacgtct 2340

tgatcaggaa ctgactaaag aaattccctt tcaccttttg cgggatttgt tggatccatc 2400

ctcacgcttc ggattccttg ctcaagagaa acatccatgc atatcgtcga cagcgttcgt 2460

tcagtcctct tccttttgtt gttggtgctg ttgttattat tgttattgtt attgctgcct 2520

attcgctcgc cagtgatgaa gaatagtcct gcctatattt gcctgtagta cattgtaaag 2580

cctacagttga cgtgtcttgt aagaccctta ttaattattgt ccataccacg acgtctc 2637

<210>5

<211>3086

<212>DNA

<213>Oryza sativa L. indica

<400>5

atattggttc tagagagatg gctctaaagg tacacagttc atgctgatac accatttgtc 60

cgtgtatagt gtatgctgta aagtacaggt ctttgcagtt tctgtctctc ttaaacaagt 120

gcgttggatt caactcactg ttcttatatc taatgagatt actggtgatt tatgttttta 180

tggcactacc aggtgttcaa ttggctgaat cggaagaagc attctaatgt cgagtattgc 240

accatcaatg agaacaaggg taaggatacc aggatatctc tcttttatgt accacatgtt 300

ttgttgttct catgttgtca actcttgttt cttctcttct atttttttcc ctgatggagt 360

agccatggaa gagaaggaag actctctgcg tgcaagtgtg actgagcaag acactgaggc 420

cctgctgctc cgtgatgtgc ttattaatgg tatacttgca attggcacgc tgggccacaa 480

tgtaaactca ctctgtcctg agtcctgtat tgaacaagat gagcccatca tcatgtgtga 540

tgagaaagtg gaacaagaga agtgcgaaga agaaaaggct gaggctaaac aggacacacc 600

agttacagca ccaagtgaac cggcatctgc tcttgagcct gccaagatgc actcatcatc 660

gatgaaagaa gacaacttca tgtgctttgt gaaggaggaa atcctaatgc atggcatgga 720

agtggaagat gttcctaaca tccaggaacg accacttctg atgttagaga aggtggagaa 780

agtgagaact acacttgccg atctatttgc tgcagaagca ttctcatcaa gtgatgcaga 840

ggataagtgt tacccgaaaa tcgtcattgt tgctggggca tccacttcaa agcctacgtc 900

gtgcatggag aagatgcatc acaagaagcc aacaaaacca acgtcaaagc cgctgaaggc 960

tacgagaaaa ttaagtcgag tatggttttc ttcgtctctg ctttatttgt taagccttgt 1020

tacatttat tttactagtc tggaatttat aacactttgt tccatggtca gtgcgccaca 1080

tcttacacat atgttttacc atatagctac aactggagta agcatttatgc atctagccag 1140

tctttagtgg taaatgatca ctgacctgga atgcaccttt ttggtttttg tcatctgtaa 1200

aataagtagg tcatgaggaa gatgttgggg aagaagatcc accccagagca gctcaatgga 1260

cgtagcaatg cagagggccc tgtcactgca taatgctagg tttgtggaca aaagtttcct 1320

ttctctatct ggcaatttat tacctagagt ttttttaaag ctgtctgttg aactatgcag 1380

ggctcaaggc ccttgtgttt tgcatacgat tttgtacctt tccaaatttt cttgattttt 1440

acttggattc cattgtttgt aataaaatta ggttaatatc tgaggtagta tttgcataga 1500

atggatacct tctaccaaag ttatatttgt ttgtgggtcc tagagctagc atgacccacg 1560

aatcacattc aagaatatag taattcgttt aggctacatt tcactaggaa tagaacatat 1620

gaatattctc aggtttaata aggcagatgc aaaaaagaac tgaatagcac aggaaagtaa 1680

tttttccatt tcaagcttct cacagtcaaa aacaagtaat ggttcaaaaa ttgaatattc 1740

tatcacctgt tgcctccatt tatgtggaac tcacaagagg gtctaagtgc tgcttgacac 1800

tctgacctgt atttaaataa aatgttatca cctattgcct ctgcttatta ttgggatctc 1860

aaaacacaat ctattactgt gtatgccttg gcatttttta aaatctgagc tgccccctta 1920

ttcttcacat tttctcagaa aaccatataa acttttagat aaatgaagct tttattgatc 1980

tcagacaatt acatcaagtt gatagaacca aactaagaac acttctggcc tctgataatg 2040

gaactgctgt ttgtttaagt agaaagaatg gtgtgtgtat tttaacggtt ggtgattggg 2100

aattgggatg cagtgaccat ggagactgtg ttcttcagaa tatttttaag aaatagtgtt 2160

ttcggtggat cctatcatgg aactgcctat ggtggaaaga gcaatacgag ttgaaacata 2220

tagggattag gaagcatggg ttcggactaa aatgcattcg ttcctgccat gatgcaaagc 2280

ttcattcgaa gcaaaatgag cacatgccat ggtacagtgg cgtgcaacga gcactgcaat 2340

tgcacacgat gtgcgtgttc tgaaaaaggc cagtggacac tagacatgca gctaatcctc 2400

ttttgatgaa ttctatcatg aaaccaatgg attcgttgaa aatctgaaca aattgatgga 2460

tttggtccca gctgtagaaa ggttggcaaa ctcttctcgc tcctgtatta ttgtaggctg 2520

gagctatgat ggaccaaatc atcagaagca aatttcacca tatataagca tctactttta 2580

tctttttctc tattttaaaa tgtgatcata gtgaagttat gtacattcta tctgcttaac 2640

gctccacatt atctaaaaaa atgcatcaca ctccatattg ggaacacgtt ggtggttctg 2700

aaccaaagtc gatctcttga tgccaatttt tttttgagct ggttgagtag tcgaactggg 2760

aacaattact gcttgaggct acaaaatttc tggccgacaa tacgtcttga tcaggaactg 2820

actaaagaaa ttccctttca ccttttgcag gatttgttgg atccatcctc acgcttcgga 2880

ttccttgctc aagagaaaca tccatgcata tcgtcgacag cgttcgttca gtcctcttcc 2940

ttttgttgtt gttgctgttg ttaattattgt tattgttatt gctgcctatt cgctcgccag 3000

tgatgaataa tagtcctgcc tatatttgcc tgtagtacat tgtaaagcta cagttgacgt 3060

gtcttgtaag accctttatta ttatg 3086

<210>6

<211>3146

<212>DNA

<213>Oryza sativa L.

<400>6

tctggctttc tcttctggtt tcatattggt tctagagaga tggctctaaa ggtacacagt 60

tagttcatgc tgatacacca tttgtccgtg tatagtgtat gctgtaaagt acaggtcttt 120

gcagtttctg tctctcttaa acaagtgcgt tggattcaac tcactgttct tatatctaat 180

gagattactg gtgattatg tttttatggc actaccaggt gttcaattgg ctgaatcgga 240

agaagcattc taatgtcgag tattgcacca tcaatgagaa caagggtaag gataccagga 300

tatctctctt ttatgtacca catgttttgt tgttctcatg ttgtcaactc ttgtttcttc 360

tcttctattt tttttcctga tggagtagcc atggaagaga aggaagactc tctgcgtgca 420

agtgtgactg agcaagacac tgaggccctg ctgctccgtg atgtgcttat taatggtata 480

cttgcaattg gcacgctggg ccacaatgta aactcactct gtcctgagtc ctgtattgaa 540

caagatgagc ccatcatcat gtgtgatgag aaagtggaac aagagaagtg cgaagaagaa 600

aaggctgagg ctaaacagga cacaccagtt acagcaccaa gtgaaccggc atctgctctt 660

gagcctgcca agatgcactc atcatcgatg aaagaagaca acttcatgtg ctttgtgaag 720

gaggaaatcc taatgcatgg catggaagtg gaagatgttc ctaacatcca ggaacgacca 780

cttctgatgt tagagaaggt ggagaaagtg agaactacac ttgccgatct atttgctgca 840

gaagcattct catcaagtga tgcagaggat aagtgttacc cgaaaatcgt cattgttgct 900

ggggcatcca cttcaaagcc tacgtcgtgc atggagaaga tgcatcacaa gaagccaaca 960

aaaccaacgt caaagccgct gaaggctaca agaaaattaa gtcgagtatg gttttcttcg 1020

tctctgcttt atttgttaag ccttgttaca ttatatttta ctagtctgga atttattaca 1080

ctttgttcca tggtcagtgc gccacatctt acacatatgt tttaccatat agctacaact 1140

ggagtaagca ttatgcatct agccagtctt tagtggtaaa tgatcactga cctggaatgc 1200

acctttttgg tttttgtcat ctgtaaaata agtaggtcat gaggaagatg ttggggaaga 1260

agatccaccc agagcagctc aatggacgta gcaatgcaga gggccctgtc actgcataat 1320

gctaggtttg tggacaaaag tttcctttct ctatctggca atttattacc tagagttttt 1380

ttaaagctgt ctgttgaact atacagggct caaggccctt gtgttttgca tacgattttg 1440

tacctttcca aattttcttg attttactt ggattccatt gtttgtaata aaattaggtt 1500

aatatctgag gtagtatttg catagaatgg ataccttcta ccaaagttat atttgtttgt 1560

gggtcctaga gctagcatga cccacgaatc attcaaga atatagtaat tcgtttaggc 1620

tacatttcac taggaataga acatatgaat attctcaggt ttaataaggc agatgcaaaa 1680

aagaactgaa tagcacagga aagtaatttt tccatttcaa gcttctcaca gtcaaaaaca 1740

agtaatggtt caaaaattga atattctatc acctgttgcc tccattattg tggaactcac 1800

aagagggtct aagtgctgct tgacactctg acctgtattt aaataaaatg ttatcaccta 1860

ttgcctctgc ttaattattgg gatctcaaaa cacaatctat tactgtgtat gccttggcat 1920

tttttaaaat ctgagctgcc cccttattct tcacattttc tcagaaaacc atataaactt 1980

ttagataaat gaagctttta ttgatctcag acaattacat caagttgata gaaccaaact 2040

aagaacactt ctggcctctg ataatggaac tgctgtttgt ttaagtagaa agaatggtgt 2100

gtgtatttta acggttggtg attgggaatt gggatgcagt gaccatggag actgtgttct 2160

tcagaatatt tttaagaaat agtgttttcg gtggatccta tcatggaact gcctatggtg 2220

gaaagagcaa tacgagttga aacatatagg gattaggaag catgggttcg gactaaaatg 2280

cattcgttcc tgccatgatg caaagcttca ttcgaagcaa aatgagcaca tgccatggta 2340

cagtggcgtg caacgagcac tgcaattgca cacgacgtgc atgttctgaa aaaggccagt 2400

ggacactaga catgcagcta atcctctttt gatgaattct atcatgaaac caatggattc 2460

gttgaaaatc tgaacaaatt gatggatttg gtcccagctg tagaaaggtt ggcaaactct 2520

tctcgctcct gtattattgt aggctggagc tatgatggac caaatcatca gaagcaaatt 2580

tcaccatata taagcatcta cttttatctt tttctctatt ttaaaatgtg atcatagtga 2640

agttatatac attctatctg cttaacgctc cacattatcc aaaaaaatgc atcacactcc 2700

atattgggaa cacgttggtg gttctgaacc aaagtcgatc tcttgatgcc aatttttttt 2760

ttgagctggt tgagtagtcg aactgggaac aattactgct tgaggctaca aaatttctgg 2820

ccgacaatac gtcttgatca ggaactgact aaagaaattc cctttcacct tttgcgggat 2880

ttgttggatc catcctcacg cttcggattc cttgctcaag agaaacatcc atgcatatcg 2940

tcgacagcgt tcgttcagtc ctcttccttt tgttgttggt gctgttgtta ttatgttat 3000

tgttattgct gcctattcgc tcgccagtga tgaagaatag tcctgcctat atttgcctgt 3060

agtacattgt aaagctacag ttgacgtgtc ttgtaagacc cttattatta ttgtccatac 3120

cacgacgtct catcatcggg ttctaa 3146

Claims (9)

1, the cDNA sequence of adjusting and controlling rice tillering angle gene is one of following nucleotide sequence:

1) SEQ ID № in the sequence table: 2 or SEQ ID №: 4 dna sequence dna;

2) SEQ ID № in the code sequence tabulation: 1 dna sequence dna;

3) under the rigorous condition of height can with SEQ ID № in the sequence table: the nucleotide sequence of 2 or SEQ ID №: the 4 dna sequence dnas hybridization that limit.

2, gene according to claim 1 is characterized in that: its cDNA has SEQ ID № in the sequence table: 2 dna sequence dna.

3, the genome sequence of adjusting and controlling rice tillering angle gene is one of following nucleotide sequence:

1) SEQ ID № in the sequence table: 3 dna sequence dna;

2) under the rigorous condition of height can with SEQ ID № in the sequence table: the nucleotide sequence of the 3 dna sequence dnas hybridization that limit.

4, the albumen of the described genes encoding of claim 1 has one of following amino acid residue sequences:

1) the SEQ ID № in the sequence table: 1;

2) with SEQ ID № in the sequence table: 1 amino acid residue sequence is through replacement, disappearance or the interpolation of one to ten amino-acid residue and the protein with adjusting and controlling rice tillering angle function.

5, albumen according to claim 4 is characterized in that: described albumen has SEQ ID № in the sequence table: 1 amino acid residue sequence.

6, contain claim 1 or 2 or 3 described expression carrier, transgenic cell line and host bacterium.

7, a kind of method of adjusting and controlling rice tillering angle is that rice tillering angle obtains regulation and control with the gene importing rice tissue or the cell of the described adjusting and controlling rice tillering angle of claim 1.

8, method according to claim 7 is characterized in that: the gene of described adjusting and controlling rice tillering angle imports explant by the plant expression vector that contains described gene; The carrier that sets out that is used to make up described plant expression vector is pCAMBIA1301-UbiN, pBI121, pBin19, pCAMBIA2301 or pCAMBIA1300.

9, method according to claim 8 is characterized in that: described plant expression vector is pCambia1301-UbiN-COM1.

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