CN1813834A - Method for preparing total flavone extract of many prickle acanthopanax - Google Patents
Method for preparing total flavone extract of many prickle acanthopanax Download PDFInfo
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- CN1813834A CN1813834A CN 200610016616 CN200610016616A CN1813834A CN 1813834 A CN1813834 A CN 1813834A CN 200610016616 CN200610016616 CN 200610016616 CN 200610016616 A CN200610016616 A CN 200610016616A CN 1813834 A CN1813834 A CN 1813834A
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- effluent
- ethanol
- acanthopanacis senticosi
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- acanthopanax
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- 239000000284 extract Substances 0.000 title claims abstract description 20
- 229930003944 flavone Natural products 0.000 title claims description 22
- 235000011949 flavones Nutrition 0.000 title claims description 22
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title description 6
- 238000000034 method Methods 0.000 title description 6
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title description 6
- 150000002212 flavone derivatives Chemical class 0.000 title description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 79
- 239000003480 eluent Substances 0.000 claims abstract description 17
- 238000000605 extraction Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000002360 preparation method Methods 0.000 claims abstract description 10
- 239000004952 Polyamide Substances 0.000 claims abstract description 9
- 239000012141 concentrate Substances 0.000 claims abstract description 9
- 239000012535 impurity Substances 0.000 claims abstract description 9
- 229920002647 polyamide Polymers 0.000 claims abstract description 9
- 238000010828 elution Methods 0.000 claims description 24
- 150000002213 flavones Chemical class 0.000 claims description 16
- 238000010992 reflux Methods 0.000 claims description 8
- 238000001694 spray drying Methods 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 2
- 230000001476 alcoholic effect Effects 0.000 claims description 2
- 206010008190 Cerebrovascular accident Diseases 0.000 abstract description 2
- OVSQVDMCBVZWGM-SJWGPRHPSA-N Hyperin Natural products O[C@H]1[C@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OVSQVDMCBVZWGM-SJWGPRHPSA-N 0.000 abstract description 2
- FVQOMEDMFUMIMO-UHFFFAOYSA-N Hyperosid Natural products OC1C(O)C(O)C(CO)OC1OC1C(=O)C2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 FVQOMEDMFUMIMO-UHFFFAOYSA-N 0.000 abstract description 2
- 208000006011 Stroke Diseases 0.000 abstract description 2
- OVSQVDMCBVZWGM-DTGCRPNFSA-N quercetin 3-O-beta-D-galactopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OVSQVDMCBVZWGM-DTGCRPNFSA-N 0.000 abstract description 2
- BBFYUPYFXSSMNV-UHFFFAOYSA-N quercetin-7-o-galactoside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC(O)=C2C(=O)C(O)=C(C=3C=C(O)C(O)=CC=3)OC2=C1 BBFYUPYFXSSMNV-UHFFFAOYSA-N 0.000 abstract description 2
- 229930003935 flavonoid Natural products 0.000 abstract 3
- 150000002215 flavonoids Chemical class 0.000 abstract 3
- 235000017173 flavonoids Nutrition 0.000 abstract 3
- 241001632410 Eleutherococcus senticosus Species 0.000 abstract 2
- 230000002490 cerebral effect Effects 0.000 abstract 1
- NQYPTLKGQJDGTI-FCVRJVSHSA-N hyperoside Natural products OC[C@H]1O[C@@H](OC2=C(Oc3cc(O)cc(O)c3[C@H]2O)c4ccc(O)c(O)c4)[C@H](O)[C@@H](O)[C@H]1O NQYPTLKGQJDGTI-FCVRJVSHSA-N 0.000 abstract 1
- 239000007921 spray Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 7
- 238000000926 separation method Methods 0.000 description 3
- -1 flavone compound Chemical class 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000003390 Chinese drug Substances 0.000 description 1
- 102000011759 adducin Human genes 0.000 description 1
- 108010076723 adducin Proteins 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 239000007909 solid dosage form Substances 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
本发明涉及一种治疗脑中风的刺五加叶总黄酮提取物的制备方法。将刺五加叶经除杂，碎断，置于提取罐中，加40％－95％乙醇回流提取2－4次，每次1－3小时，每次加乙醇的体积量L∶刺五加叶重量Kg为6－12∶1，提取液合并，浓缩，滤过，加入已处理好的聚酰胺柱中，以水洗脱至流出液近无色，再以10％－30％乙醇洗脱至流出液淡黄色，弃去上述洗脱液，再以40％－70％乙醇洗脱，收集洗脱液至流出液淡黄色，回收乙醇并浓缩，喷雾干燥，即得本发明的刺五加叶总黄酮提取物。以金丝桃苷计，总黄酮含量为50％－99％。The invention relates to a preparation method of total flavonoid extract of Acanthopanax senticosus leaves for treating cerebral apoplexy. After removing impurities, the leaves of Acanthopanax senticosus are broken, placed in an extraction tank, and refluxed with 40%-95% ethanol to extract 2-4 times, each time for 1-3 hours, the volume of ethanol added each time L: Acanthopanax The weight Kg of the added leaves is 6-12:1, the extracts are combined, concentrated, filtered, added to the treated polyamide column, eluted with water until the effluent is nearly colorless, and then washed with 10%-30% ethanol Remove until the effluent is light yellow, discard the above eluent, and then elute with 40%-70% ethanol, collect the eluate until the effluent is light yellow, recover the ethanol and concentrate, spray dry, and obtain the Acanthopanax quinciensis of the present invention. Add leaf total flavonoid extract. Calculated by hyperoside, the total flavonoid content is 50%-99%.
Description
Technical field
The invention belongs to the field of Chinese medicines, relate to the preparation method of Folium Acanthopanacis Senticosi total flavones extract.
Background technology
The extracting method that with the Radix Et Caulis Acanthopanacis Senticosi is all kinds of preparations of raw material is generally the water extract-alcohol precipitation extraction method, and the water of this method is put forward mode and compared with alcohol extracting method, and is low to the extraction ratio of flavone compound.What Song Shiyue etc. [Chinese patent, 11133176] with the Radix Et Caulis Acanthopanacis Senticosi were that the Radix Et Caulis Acanthopanacis Senticosi injection powder pin of raw material adopts is that the water extract-alcohol precipitation extraction method is extracted after the macroporous resin column separation method obtains Radix Et Caulis Acanthopanacis Senticosi extract.And macroporous adsorbent resin is because relatively poor to the selectivity of flavone compound, and the extractive total flavone content that obtains is not high.
Summary of the invention
The preparation method that the purpose of this invention is to provide Folium Acanthopanacis Senticosi total flavones extract.Preparation method of the present invention is the total flavones in the extraction separation Folium Acanthopanacis Senticosi effectively, general flavone content height in the extract, perfect technology, rationally.
The preparation method of a kind of Folium Acanthopanacis Senticosi total flavones extract of the present invention is: with Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 40%-95% alcohol reflux 2-4 time, each 1-3 hour, add alcoholic acid volume L: Folium Acanthopanacis Senticosi weight Kg was 6-12: 1 at every turn, extracting solution merges, concentrate, filter, add in the polyamide column of having handled well, closely colourless with water elution to effluent, faint yellow with the 10%-30% ethanol elution again to effluent, discard above-mentioned eluent, again with the 40%-70% ethanol elution, it is faint yellow to effluent to collect eluent, reclaim ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.In hyperin, general flavone content is 50%-99%.
Folium Acanthopanacis Senticosi total flavones extract can be treated apoplexy.Total flavones in the preparation method extraction separation Folium Acanthopanacis Senticosi of the present invention can be made any solid dosage forms with existing Chinese drug preparation technique and process, comprises capsule, tablet, powder or pill etc.
The specific embodiment
Embodiment 1:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 40% alcohol reflux 4 times, each 1 hour, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 6: 1 at every turn, extracting solution merges, concentrate, filter, add in the polyamide column of having handled well, closely colourless with water elution to effluent, faint yellow with 10% ethanol elution again to effluent, discard above-mentioned eluent, again with 40% ethanol elution, it is faint yellow to effluent to collect eluent, reclaim ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Embodiment 2:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 50% alcohol reflux 2 times, each 3 hours, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 11: 1 at every turn, extracting solution merges, concentrate, filter, add in the polyamide column of having handled well, closely colourless with water elution to effluent, faint yellow with 20% ethanol elution again to effluent, discard above-mentioned eluent, again with 50% ethanol elution, it is faint yellow to effluent to collect eluent, reclaim ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Embodiment 3:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 60% alcohol reflux 2 times, each 2.5 hours, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 12: 1 at every turn, extracting solution merges, concentrate, filter, add in the polyamide column of having handled well, closely colourless with water elution to effluent, faint yellow with 30% ethanol elution again to effluent, discard above-mentioned eluent, again with 60% ethanol elution, it is faint yellow to effluent to collect eluent, reclaim ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Embodiment 4:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 70% alcohol reflux 3 times, each 1.5 hours, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 9: 1 at every turn, extracting solution merges, and concentrates, and filters, add in the polyamide column of having handled well, closely colourless with water elution, faint yellow with 10%, 20% ethanol elution again to effluent to effluent, discard above-mentioned eluent, with 70% ethanol elution, it is faint yellow to effluent to collect eluent again, reclaims ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Embodiment 5:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 80% alcohol reflux 2 times, each 2 hours, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 10: 1 at every turn, extracting solution merges, and concentrates, and filters, add in the polyamide column of having handled well, closely colourless with water elution, faint yellow with 10%, 30% ethanol elution again to effluent to effluent, discard above-mentioned eluent, with 55% ethanol elution, it is faint yellow to effluent to collect eluent again, reclaims ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Embodiment 6:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 95% alcohol reflux 3 times, each 2 hours, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 8: 1 at every turn, extracting solution merges, concentrate, filter, add in the polyamide column of having handled well, closely colourless with water elution to effluent, faint yellow with 25% ethanol elution again to effluent, discard above-mentioned eluent, again with 65% ethanol elution, it is faint yellow to effluent to collect eluent, reclaim ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Claims (1)
1. the preparation method of a Folium Acanthopanacis Senticosi total flavones extract, it is characterized in that: with Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 40%-95% alcohol reflux 2-4 time, each 1-3 hour, add alcoholic acid volume L: Folium Acanthopanacis Senticosi weight Kg is 6-12 at every turn: 1, and extracting solution merges, and concentrates, filter, add in the polyamide column of having handled well, closely colourless with water elution, faint yellow with the 10%-30% ethanol elution again to effluent to effluent, discard above-mentioned eluent, with the 40%-70% ethanol elution, it is faint yellow to effluent to collect eluent again, reclaims ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610016616 CN1813834A (en) | 2006-03-03 | 2006-03-03 | Method for preparing total flavone extract of many prickle acanthopanax |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610016616 CN1813834A (en) | 2006-03-03 | 2006-03-03 | Method for preparing total flavone extract of many prickle acanthopanax |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1813834A true CN1813834A (en) | 2006-08-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610016616 Pending CN1813834A (en) | 2006-03-03 | 2006-03-03 | Method for preparing total flavone extract of many prickle acanthopanax |
Country Status (1)
| Country | Link |
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| CN (1) | CN1813834A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100446779C (en) * | 2007-06-20 | 2008-12-31 | 黑龙江省珍宝岛制药有限公司 | Method for preparing acanthopanax senticousus extracting liquid/extraction |
| CN102579531A (en) * | 2012-02-20 | 2012-07-18 | 山东大学威海分校 | Acanthopanax leaf extract having antioxidation |
| CN102977164A (en) * | 2012-11-05 | 2013-03-20 | 河南牧翔动物药业有限公司 | Enzyme-assistant extraction technology of hyperin in leaves of Acanthopanax senticosus Harms |
| CN104069312A (en) * | 2013-03-29 | 2014-10-01 | 天津药物研究院 | Traditional Chinese medicine composition for treating stroke, and preparation method, pharmaceutical preparation and application thereof |
| CN105476962A (en) * | 2015-12-04 | 2016-04-13 | 中国科学院长春应用化学研究所 | Acanthopanax leave flavone lipidosome and preparation method thereof |
| CN106309523A (en) * | 2015-11-01 | 2017-01-11 | 长沙博海生物科技有限公司 | Total flavonoid extract of acanthopanax senticosus harms, as well as preparation method and application thereof |
-
2006
- 2006-03-03 CN CN 200610016616 patent/CN1813834A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100446779C (en) * | 2007-06-20 | 2008-12-31 | 黑龙江省珍宝岛制药有限公司 | Method for preparing acanthopanax senticousus extracting liquid/extraction |
| CN102579531A (en) * | 2012-02-20 | 2012-07-18 | 山东大学威海分校 | Acanthopanax leaf extract having antioxidation |
| CN102977164A (en) * | 2012-11-05 | 2013-03-20 | 河南牧翔动物药业有限公司 | Enzyme-assistant extraction technology of hyperin in leaves of Acanthopanax senticosus Harms |
| CN102977164B (en) * | 2012-11-05 | 2015-09-30 | 河南牧翔动物药业有限公司 | The enzyme assisted extraction process of Quercetin 3-galactoside in a kind of Folium Acanthopanacis Senticosi |
| CN104069312A (en) * | 2013-03-29 | 2014-10-01 | 天津药物研究院 | Traditional Chinese medicine composition for treating stroke, and preparation method, pharmaceutical preparation and application thereof |
| CN106309523A (en) * | 2015-11-01 | 2017-01-11 | 长沙博海生物科技有限公司 | Total flavonoid extract of acanthopanax senticosus harms, as well as preparation method and application thereof |
| CN105476962A (en) * | 2015-12-04 | 2016-04-13 | 中国科学院长春应用化学研究所 | Acanthopanax leave flavone lipidosome and preparation method thereof |
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