CN1654435A - Composite microbial manure and method for production thereof - Google Patents
Composite microbial manure and method for production thereof Download PDFInfo
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- CN1654435A CN1654435A CN 200410065948 CN200410065948A CN1654435A CN 1654435 A CN1654435 A CN 1654435A CN 200410065948 CN200410065948 CN 200410065948 CN 200410065948 A CN200410065948 A CN 200410065948A CN 1654435 A CN1654435 A CN 1654435A
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- bacillus
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Abstract
The present invention discloses one kind of composite microbial fertilizer and its production process. The fertilizer features that it contains four kinds of microbial fermented matters of Clostridium pasteurianum, hemp soaking bacillus, phosphate decomposing giant bacillus and jelly-like bacillus silicate in the ratio of 40-60 to 25-35 to 10-20 to 2-8. The production process of the composite microbial fertilizer includes separating bacillus seed culture, fermentation, and combining the fermented products into the composite microbial fertilizer. The present invention can suit different soil types and different crops, and can improve soil, lower plantation cost and increase yield of crops, and has the use amount of 50-100 g/mou.
Description
Technical field:
The present invention relates to the fertilizer in the agrotechnique, is a kind of bio-feritlizer, specifically, is a kind of novel composite microbiological fertilizer and production method thereof.
Background technology:
Existing microbial fertilizer is monistic microbial fertilizer mostly.As: azotobacteria fertilizer, mainly form by bacterium with nitrogen fixation and fungi; And for example: phosphorus bacteria, its function are the organophosphoruss that decomposes in the soil, make it to become the phosphorus element that is easy to by plant absorbing; For another example: potassium bacterium, its function are the potassium compounds that decomposes in the soil, make it to become the available potassium that can be utilized by plant absorbing.These fertilizer all can be brought into play each self-applying when using separately, part satisfies plant to nitrogen, phosphorus, the needs of potassium three elements.They can not produce the soil compaction phenomenon that the life-time service chemical fertilizer is caused.But its deficiency is: use above-mentioned three quasi-microorganism fertilizer can only solve an aspect of plant desired nutritional separately, and cost is higher, consumption is big, is generally 3～15 kilograms of/mu (666.67m
2); Simultaneously, the three is used, again because of being difficult to the not enough or waste that the ratio of accomplishing suitably causes rate of fertilizer application.Single or the compound microbial fertilizer of life-time service facts have proved that they can only substitute below 30% of chemical fertilizer.
Summary of the invention:
Goal of the invention of the present invention provide a kind of can be at the basic condition of China's soil fertility, the basic content status of nitrogen, phosphorus, potassium three elements wherein particularly, design the plyability microbial fertilizer that contains vinelandii, phosphorus bacteria and potassium bacterium, to satisfy composite microbiological fertilizer and the production method thereof of plant to comprehensive needs of nitrogen, phosphorus, potassium.
Technical solution of the present invention is:
A kind of composite microbiological fertilizer is characterized in that: contain Clostridium baratii, soak numb genus bacillus, phosphorus decomposing bacillus megaterium and four kinds of microorganisms of bacillusmusilaginosiengineering silicate subspecies, its fermented product ratio is 40～60: 25～35: 10～20: 2～8.
Clostridium baratii described in the present invention, the fermented product ratio of soaking numb genus bacillus, phosphorus decomposing bacillus megaterium and four kinds of complex microbial communities of bacillusmusilaginosiengineering silicate subspecies are 50: 30: 15: 5.
A kind of method of producing composite microbiological fertilizer, it is characterized in that: with elite nitrogen-fixing bacteria, the potassium bacterial isolates that has the phosphorus bacteria that decomposes the organophosphorus function and can decompose potassium compound cultivates respectively, ferments, and then tunning is combined as composite microbiological fertilizer in proportion.
Elite nitrogen-fixing bacteria described in the present invention is Clostridium baratii and soaks numb genus bacillus; Phosphorus bacteria is the phosphorus decomposing bacillus megaterium; The potassium bacterium is for being bacillusmusilaginosiengineering silicate subspecies.The portfolio ratio of complex microbial community determines that through orthogonal experiment the ratio of four kinds of bacterium tunnings is: Clostridium baratii, soak numb genus bacillus, phosphorus decomposing bacillus megaterium and bacillusmusilaginosiengineering silicate subspecies=50: 30: 15: 5.
Beneficial effect of the present invention is: solved present domestic nitrogenous fertilizer (chemical fertilizer) inadequate resource and expensive problem.Reduce fertilizer cost, be beneficial to the development of agriculture production.Moisture content in its product≤10%.Total plate count 〉=8,600,000,000/gram; Total number of molds≤3.4 hundred million/gram.The ratio that this product is determined in orthogonal experiment by two kinds of vinelandii and phosphorus bacteria, potassium bacterium combines, so can replenish the deficiency of existing chemical fertilizer and microbial fertilizer unicity, it is suitable for the plant growth in China's Different Soil.Nitrogen, phosphorus, potassium fertilizer that this product substitutes more than 50% use, and can reduce planting cost, improve output.Experiment shows, uses this product can improve grain yield 5～30%, improves vegetable crop 10～40%, also can improve the quality and the local flavor of vegetables, grain.Every mu of (666.67m
2) amount of land use fertilizer of the present invention is 50～100 grams, is one of less composite microbiological fertilizer of usage quantity both at home and abroad at present.
The present invention is further illustrated below in conjunction with embodiment:
Embodiment:
Embodiment one:
One, bacterial classification
1, Clostridium baratii (Clostridium pasteurianum)
A kind in the fusobacterium (Clostridium) is movable no capsular bacterium.Oval gemma of inferior extreme formation.Decompose sugar and protein, solid oxygen effect is arranged.Do not reduce nitrate.Do not produce hydrogen sulfide.
2, soak numb genus bacillus (Bacillus macerans)
A kind in the bacillus (Bacillus).Motion, amphimicrobian.Usually produce sour aerogenesis from glucose, pectinose and N.F,USP MANNITOL.The VP feminine gender, caseinhydrolysate can not produce cyclodextrine from starch and glycogen.
3, phosphorus decomposing bacillus megaterium (Bacillus megatherium phosphaticum)
A kind in the bacillus (Bacillus).Motion forms pod membrane, and is aerobic.Produce acid from glucose, also often produce acid from pectinose and N.F,USP MANNITOL.Hydrolyzed starch does not produce lecithinase, the VP feminine gender.The organophosphorus that can decompose in the soil becomes the available rapid available phosphorus of plant.
4, potassium bacterium (bacillusmusilaginosiengineering silicate subspecies) (Bacillus mucilaginosussiliceus)
When growing on nutrient agar, bacterium colony is gel-shaped.Can utilize phosphorus potassium minerals such as silicate and phosphorus lime, and certain oxygen ability is admittedly arranged.
Two, spawn culture
1, Clostridium baratii
(1) test tube substratum (%)
Glucose 1.0, (NH
4)
2SO
40.001, KHPO
40.05, K2HPO
40.05, MgSO
47H
2O 0.02, and NaCl 0.001, FeSO
40.001, yeast extract paste 0.01, agar 1.5, peptone 0.01, CaCO
30.5, distilled water 100, PH7.0.
(2) culture temperature: 25 ± 2 ℃.
(3) liquid spawn (first class inoculum)
The same test tube of substratum does not just add agar.Cultivate down for 25 ℃.Container: shake bottle (triangular flask).
(4) liquid spawn enlarged culturing (second class inoculum)
The same liquid spawn of substratum is used aerobic seed tank culture.
(5) solid culture
Substratum: wheat bran 80%, bean cake powder 18%, rice meal 2%.Liquid spawn culture medium accounts for 50% of solid dry weight.0.14MPa following sterilization 1 hour.Insert liquid spawn below 40 ℃, inoculum size 20-25%.
(6) drying: Vacuumdrier, dry below 60 ℃, require cell count 〉=20,000,000,000/g, moisture≤10%.
(7) be ground into 60 orders.
2, soak numb genus bacillus (culture temperature 28-30 ℃)
(1) test tube substratum (%)
Proteolipid 0.5, extractum carnis 0.3, agar 1.5, NaCl 0.5, distilled water 100, PH7.0.
(2) liquid first class inoculum
Substratum does not add agar with (1), cultivates with the 500ml vial.
(3) liquid second class inoculum
Substratum is with (2), with the amphimicrobian seed tank culture.
(4) solid culture
1. substratum solids component: wheat bran 70%, bean cake powder 20%, Semen Maydis powder 10%.
2. medium liquid composition: press 50% of liquid spawn culture medium composition and add.
3. sterilization: 0.14MPa, 1 hour.
4. inoculum size: 20-25%.
Other operations require: viable count 〉=15,000,000,000/g, moisture≤10% with 1.
3, phosphorus decomposing bacillus megaterium
Operation is different from 1 place:
(1) test tube is cultivated: murphy juice 20%, sucrose 2%, agar 2%, distilled water 100%.
(2) liquid culture: same with (1), do not add agar.
(3) solid culture: with 2 they (4).
(4) viable count 〉=8,000,000,000/g, moisture≤10%.
4, potassium bacterium
Do not exist together with 1:
(1) test tube is cultivated: N.F,USP MANNITOL 1%, KH
2PO
40.02, MgSO
47H
2O 0.02, CaSO
42H
2O 0.02, and NaCl 0.02, CaCO
30.5, agar 2.0, distilled water 100.(culture temperature 25-30 ℃).
(2) liquid culture:, do not add agar with 4 they (1).
(3) solid culture: with 1 they (4).
(4) viable count 〉=2,500,000,000/g.
Three, bacterial classification ratio
Clostridium baratii: soak numb genus bacillus: phosphorus decomposing bacillus megaterium: potassium bacterium=50: 30: 15: 5.
Four, usage
Every mu of (666.67m
2) usage quantity be 50～100 the gram.Add 100 times tap water and 5 times urea in composite microbiological fertilizer of the present invention, activation is 24 hours in the water temperature below 45 ℃ more than 22 ℃, then together with chemical fertilizer (by last one year usage quantity 10～50%) be spilled in the soil.Preferably make base manure, annual use 1～3 time (weak point crop in vegetative period is 1 time, and long-living long-term crop is 1～3 time).
Five, principle
1, Clostridium baratii, soak numb genus bacillus nitrogen fixation arranged.
2, the phosphorus decomposing bacillus megaterium is separated the organophosphorus effect.
3, the potassium bacterium has the potassium decomposing effect.
4, Clostridium baratii can decompose sticking colloid substance, facilitates the formation of soil aggregate, and drought resistance function is arranged.
5, soak numb genus bacillus the effect of the germ reproduction of inhibition is arranged.
6, consumption is little but act on big principle: effectively viable bacteria is many, and the organism in the substratum can promote the breeding of four kinds of bacteriums in soil.
Six, the preparation of fertilizer:
The ratio that cultured four kinds of microorganisms are determined in orthogonal experiment is mixed, promptly Clostridium baratii, to soak numb genus bacillus, phosphorus decomposing bacillus megaterium and four kinds of fermented product ratios of bacillusmusilaginosiengineering silicate subspecies be 50: 30: 15: 5.And after stirring, the pack, composite microbiological fertilizer product of the present invention.
Embodiment two:
One, bacterial classification
1, Clostridium baratii (Clostridium pasteurianum)
2, soak numb genus bacillus (Bacillus macerans)
3, phosphorus decomposing bacillus megaterium (Bacillus megatherium phosphaticum)
4, potassium bacterium (bacillusmusilaginosiengineering silicate subspecies) (Bacillus mucilaginosussiliceus)
Four kinds of same substantially precedents of bacterial classification making method.
Two, the preparation of fertilizer:
Cultured four kinds of microorganisms are mixed in ratio that end to hand over experiment to determine, promptly Clostridium baratii, to soak numb genus bacillus, phosphorus decomposing bacillus megaterium and four kinds of fermented product ratios of bacillusmusilaginosiengineering silicate subspecies be 60: 25: 10: 5.And after stirring, the pack, composite microbiological fertilizer product of the present invention.
Three, usage
Every mu of (666.67m
2) usage quantity be 50 the gram.Add 80 times tap water and 4 times urea in composite microbiological fertilizer of the present invention, activation is 20 hours in the water temperature below 45 ℃ more than 22 ℃, then together with chemical fertilizer (by last one year usage quantity 10%) be spilled in the soil.Preferably make base manure, the annual use 1～3 time.
Embodiment three:
One, bacterial classification
1, Clostridium baratii (Clostridium pasteurianum)
2, soak numb genus bacillus (Bacillus macerans)
3, phosphorus decomposing bacillus megaterium (Bacillus megatherium phosphaticum)
4, potassium bacterium (bacillusmusilaginosiengineering silicate subspecies) (Bacillus mucilaginosussiliceus)
Four kinds of bacterial classification making methods are substantially with embodiment one.
One, the preparation of fertilizer:
The ratio that four kinds of microorganisms of cultured three classes are determined in orthogonal experiment is mixed, promptly Clostridium baratii, to soak numb genus bacillus, phosphorus decomposing bacillus megaterium and four kinds of fermented product ratios of bacillusmusilaginosiengineering silicate subspecies be 45: 35: 18: 2; And after stirring, the pack, composite microbiological fertilizer product of the present invention.
Three, usage
Every mu of (666.67m
2) usage quantity be 100 the gram.Add 120 times tap water and 6 times urea in composite microbiological fertilizer of the present invention, activation is 28 hours in the water temperature below 45 ℃ more than 22 ℃, then together with chemical fertilizer (by last one year usage quantity 50%) be spilled in the earth.Preferably make base manure, the annual use 1～3 time.
Embodiment four:
One, bacterial classification
1, Clostridium baratii (Clostridium pasteurianum)
2, soak numb genus bacillus (Bacillus macerans)
3, phosphorus decomposing bacillus megaterium (Bacillus megatherium phosphaticum)
4, potassium bacterium (bacillusmusilaginosiengineering silicate subspecies) (Bacillus mucilaginosussiliceus)
Four kinds of same substantially precedents of bacterial classification making method.
Two, the preparation of fertilizer:
The ratio that four kinds of microorganisms of cultured three classes are determined in orthogonal experiment is mixed, promptly Clostridium baratii, to soak numb genus bacillus, phosphorus decomposing bacillus megaterium and four kinds of fermented product ratios of bacillusmusilaginosiengineering silicate subspecies be 40: 35: 20: 5; And after stirring, the pack, composite microbiological fertilizer product of the present invention.
Three, usage
Every mu of (666.67m
2) usage quantity be 75 the gram.Add 101 times tap water and 5 times urea in composite microbiological fertilizer of the present invention, activation is 22 hours in the water temperature below 45 ℃ more than 22 ℃, then together with chemical fertilizer (by last one year usage quantity 30%) be spilled in the soil.Preferably make base manure, the annual use 1～3 time.
Embodiment five:
One, bacterial classification
1, Clostridium baratii (Clostridium pasteurianum)
2, soak numb genus bacillus (Bacillus macerans)
3, phosphorus decomposing bacillus megaterium (Bacillus megatherium phosphaticum)
4, potassium bacterium (bacillusmusilaginosiengineering silicate subspecies) (Bacillus mucilaginosussiliceus)
Four kinds of same substantially precedents of bacterial classification making method.
Two, the preparation of fertilizer:
The ratio that four kinds of microorganisms of cultured three classes are determined in orthogonal experiment is mixed, promptly Clostridium baratii, to soak numb genus bacillus, phosphorus decomposing bacillus megaterium and four kinds of fermented product ratios of bacillusmusilaginosiengineering silicate subspecies be 50: 30: 12: 8; And after stirring, the pack, composite microbiological fertilizer product of the present invention.
Three, usage
Every mu of (666.67m
2) usage quantity be 60 the gram.Add 90 times tap water and 5.5 times urea in composite microbiological fertilizer of the present invention, activation is 26 hours in the water temperature below 45 ℃ more than 22 ℃, then together with chemical fertilizer (by last one year usage quantity 40%) be spilled in the soil.Preferably make base manure, the annual use 1～3 time.
Claims (5)
1, a kind of composite microbiological fertilizer is characterized in that: contain Clostridium baratii, soak numb genus bacillus, phosphorus decomposing bacillus megaterium and four kinds of microorganisms of bacillusmusilaginosiengineering silicate subspecies, its fermented product ratio is 40～60: 25～35: 10～20: 2～8.
2, composite microbiological fertilizer according to claim 1 is characterized in that: Clostridium baratii, the fermented product ratio of soaking numb genus bacillus, phosphorus decomposing bacillus megaterium and four kinds of complex microbial communities of bacillusmusilaginosiengineering silicate subspecies are 50: 30: 15: 5.
3, a kind of method of producing composite microbiological fertilizer, it is characterized in that: with elite nitrogen-fixing bacteria, the potassium bacterial isolates that has the phosphorus bacteria that decomposes the organophosphorus function and can decompose potassium compound cultivates respectively, ferments, and then tunning is combined as composite microbiological fertilizer in proportion.
4, the method for production composite microbiological fertilizer according to claim 3 is characterized in that: elite nitrogen-fixing bacteria is Clostridium baratii and soaks numb genus bacillus; Phosphorus bacteria is the phosphorus decomposing bacillus megaterium; The potassium bacterium is for being bacillusmusilaginosiengineering silicate subspecies.
5, according to the method for claim 3 or 4 described production composite microbiological fertilizers, it is characterized in that: the portfolio ratio of complex microbial community determines that through orthogonal experiment the ratio of four kinds of bacterium tunnings is: Clostridium baratii, soak numb genus bacillus, phosphorus decomposing bacillus megaterium and bacillusmusilaginosiengineering silicate subspecies=50: 30: 15: 5.
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| CN 200410065948 CN1286776C (en) | 2004-12-25 | 2004-12-25 | Composite microbial manure and method for production thereof |
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| CN1286776C CN1286776C (en) | 2006-11-29 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101239847B (en) * | 2008-03-07 | 2011-04-20 | 山东省农业科学院土壤肥料研究所 | Liquid composite microbial fertilizer and its preparation method |
| CN102153380A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Method for preparing composite probiotic spraying agent |
| CN102199050A (en) * | 2010-12-15 | 2011-09-28 | 沈阳科丰牧业科技有限公司 | Composite microbial fertilizer and preparation method thereof |
| CN103570384A (en) * | 2013-10-28 | 2014-02-12 | 济源市国龙生物科技有限公司 | Compound microbial fertilizer and production method thereof |
| CN103773716A (en) * | 2013-12-28 | 2014-05-07 | 邵素英 | Efficient compound biofertilizer |
| CN104230450A (en) * | 2013-06-06 | 2014-12-24 | 张誉译 | Environmentally-friendly wheat special-purpose fertilizer |
| CN104322242A (en) * | 2014-09-16 | 2015-02-04 | 郎溪庆林生态特色农业观光园有限公司 | Cutting seedling raising method for promoting growth of yellow tea by using microorganisms |
| CN106396931A (en) * | 2016-08-31 | 2017-02-15 | 佛山市艳晖生物科技有限公司 | Compound nitrogen-fixing microorganism bacterium fertilizer and preparation method thereof |
| CN112390668A (en) * | 2020-11-19 | 2021-02-23 | 武汉木兰山水生态农业发展有限公司 | Microbial fertilizer production fermentation method |
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2004
- 2004-12-25 CN CN 200410065948 patent/CN1286776C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101239847B (en) * | 2008-03-07 | 2011-04-20 | 山东省农业科学院土壤肥料研究所 | Liquid composite microbial fertilizer and its preparation method |
| CN102153380A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Method for preparing composite probiotic spraying agent |
| CN102199050A (en) * | 2010-12-15 | 2011-09-28 | 沈阳科丰牧业科技有限公司 | Composite microbial fertilizer and preparation method thereof |
| CN102199050B (en) * | 2010-12-15 | 2013-06-12 | 沈阳科丰牧业科技有限公司 | Composite microbial fertilizer and preparation method thereof |
| CN102153380B (en) * | 2010-12-15 | 2013-07-24 | 沈阳科丰牧业科技有限公司 | Method for preparing composite probiotic spraying agent |
| CN104230450A (en) * | 2013-06-06 | 2014-12-24 | 张誉译 | Environmentally-friendly wheat special-purpose fertilizer |
| CN103570384B (en) * | 2013-10-28 | 2016-04-13 | 济源市国龙生物科技有限公司 | A kind of composite microbiological fertilizer and production method thereof |
| CN103570384A (en) * | 2013-10-28 | 2014-02-12 | 济源市国龙生物科技有限公司 | Compound microbial fertilizer and production method thereof |
| CN103773716A (en) * | 2013-12-28 | 2014-05-07 | 邵素英 | Efficient compound biofertilizer |
| CN103773716B (en) * | 2013-12-28 | 2016-01-20 | 义乌市巨晖生物科技有限公司 | A kind of high-efficiency composite biofertilizer |
| CN104322242A (en) * | 2014-09-16 | 2015-02-04 | 郎溪庆林生态特色农业观光园有限公司 | Cutting seedling raising method for promoting growth of yellow tea by using microorganisms |
| CN106396931A (en) * | 2016-08-31 | 2017-02-15 | 佛山市艳晖生物科技有限公司 | Compound nitrogen-fixing microorganism bacterium fertilizer and preparation method thereof |
| CN112390668A (en) * | 2020-11-19 | 2021-02-23 | 武汉木兰山水生态农业发展有限公司 | Microbial fertilizer production fermentation method |
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