CN1629620A - Fluorescence auxiliary detection device - Google Patents

Abstract

The present invention relates to a fluorescence auxiliary detection device, which utilizes the excitation light emitted by the light source module to illuminate the object to be detected through a collimator, a spectroscope and a first focusing lens respectively, thereby emitting the fluorescence to be detected, and receiving the generated photocurrent signal through a filter group, a second focusing lens and a light detector and transmitting it to a data processing device to perform detection and analysis operations. The device has the advantages of small size, simple structure and low production cost.

Description

Fluorescence auxiliary detection device

technical field

The invention relates to an auxiliary detection device, which is applied to the relevant fields of biomedicine, and in particular to a fluorescence auxiliary detection device.

Background technique

In recent years, biomedicine and other technologies have made major breakthroughs along with the progress of the world. The origin is the rise of the semiconductor industry, which has led to the rapid development of continuous research on related electronic components, and has driven biomedical research to a higher level.

Among them, the detection technology in the field of biomedicine is the current research focus. The existing detection method is to place the biochip on an optical disc with a data layer, and irradiate it with light of a specific wavelength, and then use the optical disc reading device to Simultaneously read the fluorescent signal emitted by the biological chip and the data layer signal of the optical disc data layer, and finally reconstruct the fluorescent signal of the two-dimensional biochip by combining the fluorescent signal and the data layer signal through the data processing unit. In addition, US Patent No. 6320660 also discloses related technologies.

In addition to the above detection methods, various detection techniques using electrophoresis (electrophoresis, EP) are also widely used. The basic principle of electrophoresis is the dissociation of any substance itself, or the adsorption of other charged particles on the surface, and it will move to a certain electrode in the electric field. As charged particles, they can be small ions, or biomacromolecules, proteins, nucleic acids, and virus particles. For example, the amino acid subunits that make up proteins are amphoteric substances, which can dissociate and charge under certain pH conditions to form a source of charge. Under the action of an electric field, the charged particles will move towards the oppositely charged electrode, which is the phenomenon of electrophoresis.

In biomedicine and other related detection fields, the principle of electrophoresis is also widely used, in which a high voltage is applied to the capillary, and at this time, the analyte filled in the capillary will produce electrophoresis; then the deoxyribose of the analyte Nucleic acid (deoxyribonucleic acid, DNA) will be combined with the added fluorescent substance, and through the irradiation of a light source such as a laser, it will produce fluorescence of different wavelengths, so that the relevant gene characteristics and concentration data of the analyte can be known, and the generated Test analysis report for research and development purposes.

However, the fluorescent detection equipment required for this capillary electrophoresis gene analysis process is usually complex in design and bulky, and the product price and related maintenance costs are quite expensive. Moreover, the purchase funds of research institutions are usually limited; therefore, this is a very big problem for many researchers. The big problem is also a subject that related industries are committed to improving.

Contents of the invention

The technical problem to be solved by the present invention is to provide a fluorescent auxiliary detection device, which has the advantages of simple structure, small volume, modular design, which can detect multiple analytes at the same time, and low price.

In order to achieve the above object, the present invention provides a fluorescent auxiliary detection device, which is used to irradiate an object to be tested and receive a detection fluorescence corresponding to the object to be tested, which is characterized in that it includes:

a light source module, used to emit an exciting light;

a collimator, mounted on one side of the light source module, for receiving and converting the excitation light so that the excitation light advances in parallel;

A beam splitter, arranged on one side of the collimating mirror, so that the excitation light traveling in parallel can be turned and refracted;

A first focusing lens, mounted on one side of the beam splitter, is used to focus the refracted excitation light and irradiate the object under test, so that the object under test emits the detection fluorescence correspondingly;

Wherein the detection fluorescence is converted by the first focusing lens and advances in a nearly parallel manner, and then passes through the beam splitter;

A filter lens group, assembled on the other side of the spectroscope, to filter stray light and background light, and only pass the detection fluorescence with a predetermined range of wavelengths;

a second focusing lens, mounted on one side of the filter lens group, to focus the detected fluorescence in a predetermined range of wavelengths; and

A photodetector is arranged on one side of the second focusing lens for receiving the focused detection fluorescence and converting the detection fluorescence into a photocurrent signal.

The above-mentioned fluorescent auxiliary detection device is characterized in that it also includes a photoelectric signal conversion module, including:

an optical signal conversion unit, used to receive the photocurrent signal and convert it into a voltage signal;

an amplifier for receiving and amplifying the voltage signal; and

A digital signal converting unit is used for converting the voltage signal into a digital signal.

The above-mentioned fluorescence auxiliary detection device is characterized in that the light source module is a laser diode.

The above-mentioned auxiliary fluorescence detection device is characterized in that the plane of the spectroscope receiving and reflecting the excitation light forms an included angle of 45 degrees with the incident direction of the excitation light.

The above-mentioned fluorescence auxiliary detection device is characterized in that the plane of the filter lens group receiving the detected fluorescence forms an included angle of 90 degrees with the incident direction of the detected fluorescence.

The above-mentioned auxiliary fluorescence detection device is characterized in that the filter group is an optical bandpass filter.

The above-mentioned auxiliary fluorescence detection device is characterized in that the beam splitter is a dual-wavelength beam splitter.

Another fluorescent auxiliary detection device of the present invention includes a light source module, a collimating mirror, a beam splitter, a first focusing lens, a filter lens group, a second focusing lens and a light detector; The light source module emits an excitation light to the collimating mirror to receive and convert the excitation light to advance in a parallel manner, and then the beam splitter turns and reflects the excitation light to the first focusing lens, and focuses and irradiates the object under test, so that The object to be tested correspondingly emits a detection fluorescence, and then passes through the first focusing lens so that the detection fluorescence advances in a nearly parallel manner, and passes through the beam splitter, is filtered by the filter lens group and limits the detection fluorescence within a predetermined range of wavelengths By using the second focusing lens to focus the detected fluorescence, and then receiving and converting it into a photocurrent signal through the photodetector.

The above-mentioned fluorescent auxiliary detection device is characterized in that it also includes a photoelectric signal conversion module, including:

an optical signal conversion unit, used to receive the photocurrent signal and convert it into a voltage signal;

an amplifier for receiving and amplifying the voltage signal; and

A digital signal converting unit is used for converting the voltage signal into a digital signal.

The above-mentioned fluorescence auxiliary detection device is characterized in that the light source module is a laser diode.

The above-mentioned fluorescence auxiliary detection device is characterized in that the plane receiving and reflecting the excitation light by the beam splitter forms an included angle of 45 degrees with the incident direction of the excitation light.

The above-mentioned fluorescence auxiliary detection device is characterized in that the plane of the filter lens group receiving the detected fluorescence forms an included angle of 90 degrees with the incident direction of the detected fluorescence.

The above-mentioned auxiliary fluorescence detection device is characterized in that the filter group is an optical bandpass filter.

The above-mentioned auxiliary fluorescence detection device is characterized in that the beam splitter is a dual-wavelength beam splitter.

According to an auxiliary fluorescence detection device disclosed in the present invention, it mainly includes a light source module, a collimating mirror, a beam splitter, a first focusing lens, a filter set, a second focusing lens and a light detector. The light source module is used to emit excitation light, and the collimating mirror is assembled on one side of the light source module to receive and convert the excitation light so that the excitation light advances in a parallel manner. The beam splitter is arranged on one side of the collimating mirror, and forms an included angle of 45 degrees with the advancing direction of the excitation light, so that the excitation light turns and reflects. The first focusing lens is installed on one side of the beam splitter to focus the reflected and deflected excitation light to irradiate the object under test, and the object under test correspondingly emits detection fluorescence.

Then, the detection fluorescence will be collected by the first focusing lens and travel nearly parallel, and then pass through the beam splitter. The optical filter assembly is installed on one side of the spectroscope, which can receive the detection fluorescence passing through the spectroscope, and block stray light so that the wavelength of the transmitted detection fluorescence is within a certain range. The second detection lens is used to focus the filtered detection fluorescence, and finally receive the filtered detection fluorescence through the photodetector arranged on one side of the second focus lens and convert it into a photocurrent signal. The photocurrent signal can be converted into a digital signal through the photoelectric signal conversion module and transmitted to a data processing device such as a computer for analysis and detection.

The fluorescence auxiliary detection device of the present invention can be applied to various types of fluorescence detection equipment such as capillary electrophoresis gene analyzers, gene chips, and protein chips. Advantages, and easy access to related parts can effectively reduce production costs.

The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but not as a limitation of the present invention.

Description of drawings

Fig. 1 is the schematic diagram of fluorescence auxiliary detection device of the present invention;

Fig. 2 is a schematic diagram of the fluorescence auxiliary detection device and the photoelectric signal conversion module of the present invention; and

Fig. 3 is a schematic diagram of the use of the modular fluorescence auxiliary detection device of the fluorescence auxiliary detection device of the present invention.

Detailed ways

According to a fluorescent auxiliary detection device disclosed in the present invention, please refer to FIG. 1 and FIG. 2 , which mainly includes a light source module 10, a collimating mirror 20, a beam splitter 30, a first focusing lens 40, an object under test 50, and a filter The lens group 60 , the second focusing lens 70 and the light detector 80 . Among them, the light source module 10 can be implemented in many types, and most commercially available products use gas lasers and continuous-spectrum mercury lamps. However, this type of light source module is expensive, and the service life of the mercury lamp is short, so the present invention recommends using a laser diode (Laser Diode) which is much cheaper and has similar functions as the light source module 10 . The light source module 10 is used to emit excitation light, and a collimator 20 is mounted on one side of the light source module 10 for receiving and converting the excitation light so that the excitation light advances in a parallel manner. The beam splitter 30 is placed obliquely on one side of the beam splitter, so that the parallel advancing excitation light turns to refraction. Because the beam splitter 30 has different characteristics and the slope of its installation according to its product specifications, the present invention adopts a dual-wavelength beam splitter (Dichroic Mirror), the plane receiving the excitation light is at an angle of 45 degrees to the incident direction of the excitation light. The first focusing lens 40 is an aspheric objective lens placed on one side of the beam splitter and on the optical path of the refracted excitation light for focusing the refracted excitation light on the object 50 to be tested. The object to be tested 50 in this preferred embodiment is applied with an external high voltage to cause electrophoresis to occur in the filling inside, so when the excitation light is irradiated, it will correspondingly emit detection fluorescence. In other words, after the excitation light is irradiated on the object 50 to be tested, a Stoke's Shift effect will be generated, and a detection fluorescence with a longer wavelength than the excitation light will be radiated. Then the detected fluorescence will be collected again by the first focusing lens 40 to advance in a nearly parallel manner, and pass through the beam splitter 30 in an almost completely transparent manner.

The optical filter group 60 is assembled on the other side of the beam splitter. The purpose of the optical filter group 60 is to block the entry of stray light and background light, and only allow specific wavelengths to detect fluorescence to penetrate. For the wavelength range of detection fluorescence Qualified to meet assay analysis requirements. In addition, the optical filter group 60 may be composed of a plurality of optical band pass filters (Optical Band Pass Filter) according to the detection requirements. Generally speaking, the optical filter group 60 receives the plane of the detected fluorescence and the incident direction of the detected fluorescence It is at an angle of 90 degrees. The second focusing lens 70 is a kind of aspheric objective lens, which is assembled on the other side of the filter lens group 60, and is used to focus the detected fluorescence having a predetermined range of wavelengths after being filtered by the filter lens group 60, and finally configured The photodetector 80 on one side of the second focusing lens receives and converts the detected fluorescence into a photocurrent signal. The photodetector 80 can be replaced by various types, such as a commercially available photomultiplier tube or a low-noise photodetector (Photo Diode) with the same product function. However, the photomultiplier tube has a large volume and is quite expensive, so a low-noise photodetector (Photo Diode) with low operating voltage, long service life and low price is the first choice.

As shown in FIG. 2 , since the photocurrent signal provided by the photodetector 80 cannot be directly read by the data processing device 120 , it must be completed through an additional photoelectric signal conversion module. The photoelectric signal conversion module is composed of an optical signal conversion unit 90 , an amplifier 100 , and a digital signal conversion unit 110 . The optical signal conversion unit 90 is used to receive the photocurrent signal transmitted by the photodetector 80 and convert it into a voltage signal, then receive and amplify the voltage signal through the amplifier 100, and finally convert the voltage signal through the digital signal conversion unit 110 The signal is converted into a digital signal; and the digital signal can be accepted by data processing devices such as a computer, an electrophoretic gene analyzer, etc., and can detect and analyze the detection fluorescence emitted by the object 50 to be tested.

The fluorescent auxiliary detection device provided by the present invention can also be designed in a modular manner. As shown in FIG. 50 performs related fluorescence detection and analysis, and transmits the relevant data generated by the detected fluorescence of each analyte 50 to the data processing device 120 for detection and analysis.

Certainly, the present invention also can have other multiple embodiments, without departing from the spirit and essence of the present invention, those skilled in the art can make various corresponding changes and deformations according to the present invention, but these corresponding Changes and deformations should belong to the scope of protection of the appended claims of the present invention.

Claims (14)

1, a kind of fluoroscopic assist pick-up unit, in order to shine a determinand and receive this determinand corresponding distribute one detect fluorescence, it is characterized in that, include:

One light source module is in order to launch an exciting light;

One collimating mirror is assemblied in a side of this light source module, in order to receive and to change this exciting light, so that this exciting light advances with parallel mode;

One spectroscope is arranged at a side of this collimating mirror, so that parallel this exciting light that advances turns to refraction;

One first condenser lens is assemblied in this spectroscopical side, focuses on this determinand of irradiation in order to this exciting light after will reflecting, and makes this determinand correspondence distribute this detection fluorescence;

Wherein this detection fluorescence is to see through this first condenser lens conversion and to advance near parallel mode, penetrate this spectroscope again;

One optical filtering group is assemblied in this spectroscopical opposite side, filtering parasitic light and bias light, and passes through only for this detection fluorescence of a preset range wavelength;

One second condenser lens is assemblied in a side of this optical filtering group, focuses in order to this detection fluorescence with the preset range wavelength; And

One optical detector is equipped on a side of this second condenser lens, detects fluorescence in order to behind the collectiong focusing this, and to change this detection fluorescence be a photocurrent signal.

2, fluoroscopic assist pick-up unit according to claim 1 is characterized in that, also includes a photoelectricity signal modular converter, includes:

One smooth signal conversion unit is in order to receive this photocurrent signal and to be converted to a voltage signal;

One amplifier is in order to receive and to amplify this voltage signal; And

One digital signal converting unit is in order to be converted to a digital signal with this voltage signal.

3, fluoroscopic assist pick-up unit according to claim 1 is characterized in that, this light source module is a laser diode.

4, fluoroscopic assist pick-up unit according to claim 1 is characterized in that, this spectroscope receives reflecting the plane of this exciting light, is 45 degree angles with the incident direction of this exciting light.

5, fluoroscopic assist pick-up unit according to claim 1 is characterized in that, this optical filtering group of received should detect the plane of fluorescence, is 90 with the incident direction of this detections fluorescence and spends angles.

6, fluoroscopic assist pick-up unit according to claim 1 is characterized in that, this optical filtering group is an optical band pass optical filtering.

7, fluoroscopic assist pick-up unit according to claim 1 is characterized in that, this spectroscope is a pair of measuring spectroscope.

8, a kind of fluoroscopic assist pick-up unit includes a light source module, a collimating mirror, a spectroscope, one first condenser lens, an optical filtering group, one second condenser lens and an optical detector; Wherein this light source module is launched an exciting light to this collimating mirror and is received and change this exciting light and advance with parallel mode, continuous this exciting light being turned to by this spectroscope reflexes to this first condenser lens, and this determinand of focusing irradiation, cause this determinand correspondence to distribute one and detect fluorescence, see through this first condenser lens again and make this detection fluorescence be near parallel advancing, and penetrate this spectroscope filtered and limited a preset range wavelength by this optical filtering group detection fluorescence and pass through, utilize this second condenser lens should detect fluorescence and focus on, receive by this optical detector again and be converted to a photocurrent signal.

9, fluoroscopic assist pick-up unit according to claim 8 is characterized in that, also includes a photoelectricity signal modular converter, includes:

One smooth signal conversion unit is in order to receive this photocurrent signal and to be converted to a voltage signal;

One amplifier is in order to receive and to amplify this voltage signal; And

One digital signal converting unit is in order to be converted to a digital signal with this voltage signal.

10, fluoroscopic assist pick-up unit according to claim 8 is characterized in that, this light source module is a laser diode.

11, fluoroscopic assist pick-up unit according to claim 8 is characterized in that, this spectroscope receives the plane of this exciting light of reflection, is 45 degree angles with the incident direction of this exciting light.

12, fluoroscopic assist pick-up unit according to claim 8 is characterized in that, this optical filtering group of received should detect the plane of fluorescence, is 90 with the incident direction of this detections fluorescence and spends angles.

13, fluoroscopic assist pick-up unit according to claim 8 is characterized in that, this optical filtering group is an optical band pass optical filtering.

14, fluoroscopic assist pick-up unit according to claim 8 is characterized in that, this spectroscope is a pair of measuring spectroscope.

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