CN1616673A - Enzymatic Determination of Blood Ammonia Kit - Google Patents
Enzymatic Determination of Blood Ammonia Kit Download PDFInfo
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- CN1616673A CN1616673A CNA2004100439000A CN200410043900A CN1616673A CN 1616673 A CN1616673 A CN 1616673A CN A2004100439000 A CNA2004100439000 A CN A2004100439000A CN 200410043900 A CN200410043900 A CN 200410043900A CN 1616673 A CN1616673 A CN 1616673A
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- lactose
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- ketoglutarate
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 title claims abstract description 24
- 239000008280 blood Substances 0.000 title claims abstract description 15
- 210000004369 blood Anatomy 0.000 title claims abstract description 15
- 229910021529 ammonia Inorganic materials 0.000 title claims abstract description 12
- 230000002255 enzymatic effect Effects 0.000 title 1
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims abstract description 38
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims abstract description 19
- 239000008101 lactose Substances 0.000 claims abstract description 19
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 claims abstract description 17
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229920001213 Polysorbate 20 Polymers 0.000 claims abstract description 12
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims abstract description 12
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims abstract description 12
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 claims abstract description 10
- 102000016901 Glutamate dehydrogenase Human genes 0.000 claims abstract description 10
- 102000004190 Enzymes Human genes 0.000 claims abstract description 6
- 108090000790 Enzymes Proteins 0.000 claims abstract description 6
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 claims description 34
- 229940054269 sodium pyruvate Drugs 0.000 claims description 17
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 claims description 15
- 238000006911 enzymatic reaction Methods 0.000 claims description 11
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 9
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 9
- 235000013922 glutamic acid Nutrition 0.000 claims description 9
- 239000004220 glutamic acid Substances 0.000 claims description 9
- 101710088194 Dehydrogenase Proteins 0.000 claims description 8
- 239000007983 Tris buffer Substances 0.000 claims description 7
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 5
- 238000006356 dehydrogenation reaction Methods 0.000 claims 1
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 206010018910 Haemolysis Diseases 0.000 abstract description 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 abstract description 2
- 230000008588 hemolysis Effects 0.000 abstract description 2
- 229950006238 nadide Drugs 0.000 abstract description 2
- BWLBGMIXKSTLSX-UHFFFAOYSA-N 2-hydroxyisobutyric acid Chemical compound CC(C)(O)C(O)=O BWLBGMIXKSTLSX-UHFFFAOYSA-N 0.000 abstract 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- HWXBTNAVRSUOJR-UHFFFAOYSA-N alpha-hydroxyglutaric acid Natural products OC(=O)C(O)CCC(O)=O HWXBTNAVRSUOJR-UHFFFAOYSA-N 0.000 abstract 1
- 229940009533 alpha-ketoglutaric acid Drugs 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
- 229960000281 trometamol Drugs 0.000 abstract 1
- XJLXINKUBYWONI-NNYOXOHSSA-N NADP zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-N 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- RTBFRGCFXZNCOE-UHFFFAOYSA-N 1-methylsulfonylpiperidin-4-one Chemical compound CS(=O)(=O)N1CCC(=O)CC1 RTBFRGCFXZNCOE-UHFFFAOYSA-N 0.000 description 1
- KPGXRSRHYNQIFN-UHFFFAOYSA-L 2-oxoglutarate(2-) Chemical compound [O-]C(=O)CCC(=O)C([O-])=O KPGXRSRHYNQIFN-UHFFFAOYSA-L 0.000 description 1
- JFCQEDHGNNZCLN-UHFFFAOYSA-N anhydrous glutaric acid Natural products OC(=O)CCCC(O)=O JFCQEDHGNNZCLN-UHFFFAOYSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
技术领域：Technical field:
本发明涉及一种试剂盒，具体涉及一种酶法测定血氨试剂盒。The invention relates to a kit, in particular to a kit for measuring blood ammonia by enzymatic method.
背景技术：Background technique:
血氨测定有扩散法、离子交换法、离子选择电极法和酶法，前三种方法由于测定繁琐，难于在临床上大规模开展，而酶法具有特异性高、微量、快速、准确的特点，但是目前文献介绍的酶法测定血氨方法，只适合于少量科研使用，因为该方法使用的酶与辅酶只适于短期使用，稳定性差，难于在各医院化验室大批量长期使用。There are diffusion method, ion exchange method, ion selective electrode method and enzymatic method for the determination of blood ammonia. The first three methods are difficult to carry out on a large scale in clinical practice due to the cumbersome determination, while the enzymatic method has the characteristics of high specificity, trace amount, rapidity and accuracy. However, the enzymatic method for measuring blood ammonia introduced in the current literature is only suitable for a small amount of scientific research, because the enzymes and coenzymes used in this method are only suitable for short-term use and have poor stability, making it difficult to use them in large quantities in various hospital laboratories for a long time.
发明内容：Invention content:
本发明的目的是提供一种能够长期保存的酶法测定血氨试剂盒。本发明的试剂盒由三羟甲基氨基甲烷、吐温-20、叠氮钠、乳糖、谷氨酸脱氢酶、α-酮戊二酸、丙酮酸钠、还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)组分构成，其中各组分的含量为：三羟甲基氨基甲烷50～150mmol、吐温-200.1～2ml、叠氮钠0.5～2g、乳糖5～50g、谷氨酸脱氢酶500～1500u、α-酮戊二酸2～20mmol、丙酮酸钠1～10mmol、还原型烟酰胺腺嘌呤二核苷酸磷酸0.1～1mmol。本发明的三羟甲基氨基甲烷、吐温-20对NADPH起保护作用；叠氮钠、乳糖对谷氨酸脱氢酶有保护作用。使用本发明的试剂盒，调节最佳pH＝8.0，各成分经溶解后分装、冷冻干燥，有效期可达两年(冷藏)，复溶后，室温稳定一周，冷藏稳定一个月，抗干扰能力强，中等程度溶血、乳摩血、血糖20mmol/l以下，胆红素100μmol/l以下，对测定不产生干扰，冻干试剂冷藏一年，试剂盒中酶活力损失小于5％，NADPH损失小于10％，线性达200μmol/l，批内CV＝3.8％，批间CV＝5.1％，回收率为96.1％。The purpose of the present invention is to provide a long-term preservation kit for measuring blood ammonia by enzymatic method. The kit of the present invention consists of trishydroxymethylaminomethane, Tween-20, sodium azide, lactose, glutamate dehydrogenase, α-ketoglutarate, sodium pyruvate, reduced nicotinamide adenine dinuclear NADPH component, the content of each component is: trishydroxymethylaminomethane 50-150mmol, Tween-200.1-2ml, sodium azide 0.5-2g, lactose 5-50g, glutamic acid Dehydrogenase 500~1500u, α-ketoglutarate 2~20mmol, sodium pyruvate 1~10mmol, reduced nicotinamide adenine dinucleotide phosphate 0.1~1mmol. Trishydroxymethylaminomethane and Tween-20 of the present invention have protective effects on NADPH; sodium azide and lactose have protective effects on glutamic acid dehydrogenase. Using the kit of the present invention, the optimal pH=8.0 is adjusted, each component is subpackaged and freeze-dried after dissolving, and the validity period can reach two years (refrigerated). After redissolving, the room temperature is stable for one week, and the refrigeration is stable for one month. Strong, moderate degree of hemolysis, breast rubbing blood, blood sugar below 20mmol/l, bilirubin below 100μmol/l, no interference to the determination, freeze-dried reagents refrigerated for one year, the loss of enzyme activity in the kit is less than 5%, and the loss of NADPH is less than 10%, linear up to 200 μmol/l, intra-assay CV=3.8%, inter-assay CV=5.1%, recovery rate 96.1%.
具体实施方式：Detailed ways:
具体实施方式一：本实施方式的试剂盒由三羟甲基氨基甲烷、吐温-20、叠氮钠、乳糖、谷氨酸脱氢酶、α-酮戊二酸、丙酮酸钠、还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)组分构成，其中各组分的含量为：三羟甲基氨基甲烷50～150mmol、吐温-200.1～2ml、叠氮钠0.5～2g、乳糖5～50g、谷氨酸脱氢酶500～1500u(u为酶活力的国际单位)、α-酮戊二酸2～20mmol、丙酮酸钠1～10mmol、还原型烟酰胺腺嘌呤二核苷酸磷酸0.1～1mmol，取蒸馏水，使用前将蒸馏水加热至沸腾，密封备用；将三羟甲基氨基甲烷、吐温-20、叠氮钠、乳糖、α-酮戊二酸和丙酮酸钠溶解于上述蒸馏水中，调节pH＝8.0，然后再向其中加入谷氨酸脱氢酶和NADPH，定容、分装，再经过冷冻干燥即得成品，有效期达两年。Specific embodiment one: the kit of this embodiment is composed of trishydroxymethylaminomethane, Tween-20, sodium azide, lactose, glutamate dehydrogenase, α-ketoglutarate, sodium pyruvate, reduced Nicotinamide adenine dinucleotide phosphate (NADPH) components, the content of each component is: Tris-hydroxymethylaminomethane 50-150mmol, Tween-200.1-2ml, sodium azide 0.5-2g, lactose 5 ～50g, glutamate dehydrogenase 500～1500u (u is the international unit of enzyme activity), α-ketoglutarate 2～20mmol, sodium pyruvate 1～10mmol, reduced nicotinamide adenine dinucleotide phosphate 0.1 ~ 1mmol, take distilled water, heat the distilled water to boiling before use, seal it for later use; dissolve Tris, Tween-20, sodium azide, lactose, α-ketoglutarate and sodium pyruvate in the above Adjust the pH to 8.0 in distilled water, then add glutamic acid dehydrogenase and NADPH to it, constant volume, sub-package, and then freeze-dry to obtain the finished product, with a validity period of two years.
具体实施方式二：本实施方式与具体实施方式一不同的是，各组分的含量为：三羟甲基氨基甲烷55～90mmol、吐温-200.2～0.8ml、叠氮钠0.6～1.2g、乳糖6～25g、谷氨酸脱氢酶600～900u、α-酮戊二酸5～10mmol、丙酮酸钠2～5mmol、还原型烟酰胺腺嘌呤二核苷酸磷酸0.2～0.5mmol。Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the content of each component is: trishydroxymethylaminomethane 55～90mmol, Tween-200.2～0.8ml, sodium azide 0.6～1.2g, Lactose 6-25g, glutamate dehydrogenase 600-900u, α-ketoglutarate 5-10mmol, sodium pyruvate 2-5mmol, reduced nicotinamide adenine dinucleotide phosphate 0.2-0.5mmol.
具体实施方式三：本实施方式与具体实施方式一不同的是，各组分的含量为：三羟甲基氨基甲烷100～140mmol、吐温-201.0～1.8ml、叠氮钠1.3～1.9g、乳糖30～45g、谷氨酸脱氢酶1000～1400u、α-酮戊二酸12～18mmol、丙酮酸钠6～9mmol、还原型烟酰胺腺嘌呤二核苷酸磷酸0.6～0.9mmol。Specific embodiment three: the difference between this embodiment and specific embodiment one is that the content of each component is: trishydroxymethylaminomethane 100～140mmol, Tween-201.0～1.8ml, sodium azide 1.3～1.9g, Lactose 30-45g, glutamate dehydrogenase 1000-1400u, α-ketoglutarate 12-18mmol, sodium pyruvate 6-9mmol, reduced nicotinamide adenine dinucleotide phosphate 0.6-0.9mmol.
具体实施方式四：本实施方式试剂盒中各组分的含量为：三羟甲基氨基甲烷60mmol、吐温-200.4ml、叠氮钠0.7g、乳糖7g、谷氨酸脱氢酶650u、α-酮戊二酸6mmol、丙酮酸钠3mmol、还原型烟酰胺腺嘌呤二核苷酸磷酸0.4mmol。Specific embodiment four: The content of each component in the kit of this embodiment is: trishydroxymethylaminomethane 60mmol, Tween-200.4ml, sodium azide 0.7g, lactose 7g, glutamic acid dehydrogenase 650u, α - 6 mmol of ketoglutarate, 3 mmol of sodium pyruvate, and 0.4 mmol of reduced nicotinamide adenine dinucleotide phosphate.
具体实施方式五：本实施方式试剂盒中各组分的含量为：三羟甲基氨基甲烷135mmol、吐温-201.7ml、叠氮钠1.8g、乳糖43g、谷氨酸脱氢酶1350u、α-酮戊二酸17mmol、丙酮酸钠8mmol、还原型烟酰胺腺嘌呤二核苷酸磷酸0.8mmol。Specific embodiment five: the content of each component in the kit of this embodiment is: trishydroxymethylaminomethane 135mmol, Tween-201.7ml, sodium azide 1.8g, lactose 43g, glutamic acid dehydrogenase 1350u, α - 17 mmol of ketoglutaric acid, 8 mmol of sodium pyruvate, and 0.8 mmol of reduced nicotinamide adenine dinucleotide phosphate.
具体实施方式六：本实施方式试剂盒中各组分的含量为：三羟甲基氨基甲烷100mmol、吐温-201ml、叠氮钠1g、乳糖20g、谷氨酸脱氢酶1000u、α-酮戊二酸10mmol、丙酮酸钠5mmol、还原型烟酰胺腺嘌呤二核苷酸磷酸0.24mmol。Specific embodiment six: the content of each component in the kit of this embodiment is: trishydroxymethylaminomethane 100mmol, Tween-201ml, sodium azide 1g, lactose 20g, glutamic acid dehydrogenase 1000u, α-ketone Glutaric acid 10mmol, sodium pyruvate 5mmol, reduced nicotinamide adenine dinucleotide phosphate 0.24mmol.
Claims (6)
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| CNB2004100439000A CN1256442C (en) | 2004-09-27 | 2004-09-27 | Blood ammonia reagent kit for enzyme detection |
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| CNB2004100439000A CN1256442C (en) | 2004-09-27 | 2004-09-27 | Blood ammonia reagent kit for enzyme detection |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101929318A (en) * | 2009-06-26 | 2010-12-29 | 大港油田集团有限责任公司 | Stage cementing device |
| CN102863495A (en) * | 2011-07-06 | 2013-01-09 | 上海执诚生物科技股份有限公司 | Stable composition containing NAD+ or NADH |
| CN102994612A (en) * | 2012-12-24 | 2013-03-27 | 北京利德曼生化股份有限公司 | Liquid monomer reagent for determining content of ammonia in blood serum, preparation method and application thereof |
| CN103543117A (en) * | 2013-10-21 | 2014-01-29 | 大连市沙河口区中小微企业服务中心 | Measurement method of ammonia content in serum |
| CN104374906A (en) * | 2014-11-28 | 2015-02-25 | 山东博科生物产业有限公司 | Serum ammonia detection reagent |
| CN104422666A (en) * | 2013-09-09 | 2015-03-18 | 中国科学院沈阳应用生态研究所 | Analysis method for detecting activity of glutamate dehydrogenase in soil |
-
2004
- 2004-09-27 CN CNB2004100439000A patent/CN1256442C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101929318A (en) * | 2009-06-26 | 2010-12-29 | 大港油田集团有限责任公司 | Stage cementing device |
| CN102863495A (en) * | 2011-07-06 | 2013-01-09 | 上海执诚生物科技股份有限公司 | Stable composition containing NAD+ or NADH |
| CN102994612A (en) * | 2012-12-24 | 2013-03-27 | 北京利德曼生化股份有限公司 | Liquid monomer reagent for determining content of ammonia in blood serum, preparation method and application thereof |
| CN104422666A (en) * | 2013-09-09 | 2015-03-18 | 中国科学院沈阳应用生态研究所 | Analysis method for detecting activity of glutamate dehydrogenase in soil |
| CN103543117A (en) * | 2013-10-21 | 2014-01-29 | 大连市沙河口区中小微企业服务中心 | Measurement method of ammonia content in serum |
| CN104374906A (en) * | 2014-11-28 | 2015-02-25 | 山东博科生物产业有限公司 | Serum ammonia detection reagent |
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| Publication number | Publication date |
|---|---|
| CN1256442C (en) | 2006-05-17 |
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