CN1583043A - Duyiwei extract and its preparation and use - Google Patents
Duyiwei extract and its preparation and use Download PDFInfo
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- CN1583043A CN1583043A CN 200410022734 CN200410022734A CN1583043A CN 1583043 A CN1583043 A CN 1583043A CN 200410022734 CN200410022734 CN 200410022734 CN 200410022734 A CN200410022734 A CN 200410022734A CN 1583043 A CN1583043 A CN 1583043A
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- lamiophlomidis rotatae
- radix lamiophlomidis
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- rotatae extract
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Abstract
An extract of lamiophlomis herb is disclosed, which has antalgic, inflammation-relieving and blood-circulating functions. Its preparing process and application are also disclosed.
Description
Affiliated technical field
The present invention relates to a kind of Chinese medicine extract, more particularly, the present invention relates to a kind of effective site of from the Chinese medicine Radix Lamiophlomidis Rotatae, extracting.The invention still further relates to the preparation method and the application thereof of this Chinese medicine extract.
Background technology
Traumatic wounds waits as fracture and rheumatism can produce symptoms such as pain, inflammation.Traumatic wounds needs earlier fixedly wound site as treatment of fractures, use analgesia, antiphlogistic medicine then, preferably uses the medicine with the effect of invigorating blood circulation simultaneously; Rheumatism is a kind of chronic disease, and cardinal symptom is pain, inflammation, and its treatment need be taken analgesia, anti-inflammatory drug for a long time, and it is best to take blood circulation promoting medicine simultaneously.
Existing treatment traumatic wounds medicine, chemical medicine is generally the antipyretic analgesic with analgesia and antiinflammatory action, do not have the effect of invigorating blood circulation, and the chemicals side effect is bigger; The pharmaceutical chemistry medicated bag of existing treatment rheumatism is drawn together antipyretic analgesic and partial immunity inhibitor, do not have the effect of invigorating blood circulation equally, and side effect is bigger; Compound Chinese medicinal preparation contains mostly that the taste of Chinese medicine number is more, and effective ingredient is unclear, and dose is bigger, and action intensity is not enough, and curative effect is undesirable.
The Chinese medicine Radix Lamiophlomidis Rotatae is the dry herb of labiate Radix Lamiophlomidis Rotatae Lamiophlomis rotata (Benth.) Kudo..Health Tibetan medicine standard promulgated by the ministries or commissions of the Central Government is recorded Radix Lamiophlomidis Rotatae and is had bone and muscle strengthening, the yellow fluid reducing effect.Be used for fracture contusion, bones and muscles pain, grasserie.
Summary of the invention
Purpose of the present invention just provides a kind of curative effect height, toxic and side effects is low, taking dose is little has analgesia, antiinflammatory, the Chinese medicine Radix Lamiophlomidis Rotatae extract of the effect of invigorating blood circulation.
Another object of the present invention provides a kind of preparation method of above-mentioned Radix Lamiophlomidis Rotatae extract.
A further object of the invention application in the medicine of effect that provides above-mentioned Radix Lamiophlomidis Rotatae extract and have analgesia, antiinflammatory, invigorates blood circulation in preparation.
The technical solution adopted for the present invention to solve the technical problems is: a kind of Radix Lamiophlomidis Rotatae extract, it is the extract that contains total glycosides and total flavones that extracts from the Chinese medicine Radix Lamiophlomidis Rotatae.
The total content of total glycosides and total flavones is having effect preferably in the described Radix Lamiophlomidis Rotatae extract more than 50%.
Total glycosides content is more than 20% in the described Radix Lamiophlomidis Rotatae extract, and general flavone content is better more than 10%.
Total glycosides content is more than 40% in the described Radix Lamiophlomidis Rotatae extract, and general flavone content is better more than 20%.
The preparation method of above-mentioned Radix Lamiophlomidis Rotatae extract comprises the steps:
(1) preparation Radix Lamiophlomidis Rotatae crude extract: get the raw medicinal material Radix Lamiophlomidis Rotatae or pulverize after Radix Lamiophlomidis Rotatae, extract with decoction and alcohol sedimentation technique or ethanol extraction method, extracting solution, this extracting solution is condensed into rare extractum, promptly get the Radix Lamiophlomidis Rotatae crude extract; Wherein the used ethanol of ethanol extraction method is that concentration is the ethanol of 30-95%;
(2) refining: that the Radix Lamiophlomidis Rotatae crude extract that (1) step makes is crossed macroporous adsorbent resin, the low-concentration ethanol eluting of elder generation's water or 1%-30%, the eluent of this water or low-concentration ethanol is discarded need not, remove the impurity that is dissolved in polar solvent, the higher concentration ethanol elution of reuse 40%-95%, collect this higher concentration ethanol elution, the higher concentration ethanol elution drying with behind the recovery ethanol promptly gets the Radix Lamiophlomidis Rotatae extract that contains total glycosides and total flavones.
If necessary, through the Radix Lamiophlomidis Rotatae extract that contains total glycosides and total flavones that above-mentioned (2) one-step refining makes, available n-butyl alcohol or ethyl acetate are further purified processing, to make the higher Radix Lamiophlomidis Rotatae extract of total glycosides and general flavone content.
Wherein the macroporous adsorbent resin described in (2) step hangs down polar macroporous adsorbent resin in being, as D101, D201, D301, AB-8, X-5 etc.
Wherein the drying means described in (2) step can be oven dry or drying under reduced pressure or spray drying or lyophilization etc.
The application of above-mentioned Radix Lamiophlomidis Rotatae extract, described Radix Lamiophlomidis Rotatae extract is used to prepare have analgesia, antiinflammatory, the medicine of the effect of invigorating blood circulation, describedly have analgesia, antiinflammatory, contain the above-mentioned Radix Lamiophlomidis Rotatae extract of effective dose in the medicine of the effect of invigorating blood circulation.
Describedly have analgesia, antiinflammatory, the dosage form of the medicine of the effect of invigorating blood circulation can be any existing pharmaceutical dosage form in injection, transfusion, powder pin, drop pill, tablet, slow releasing tablet, capsule, soft capsule, the granule.
The above-mentioned medicine that contains Radix Lamiophlomidis Rotatae extract is mainly used in postoperative recovery, also is used for the treatment of: soft tissue, joint and waist are dampened, traumatic injury, and fracture of ossiculum, wound, rheumatic arthritis etc. are pain caused, blood stasis, inflammation and cardiovascular and cerebrovascular disease.
Compared with prior art, the invention has the beneficial effects as follows: prove that through pharmacodynamics and toxicology test this Radix Lamiophlomidis Rotatae extract has analgesia preferably, antiinflammatory, the effect of invigorating blood circulation, toxic and side effects is less; Its effective ingredient is clear and definite, promptly wherein contained total glycosides and total flavones, and total glycosides and general flavone content are high more, other impurity components are then few more, can reduce dose, also can reduce toxic and side effects to a certain extent, more help guaranteeing the safety and the effectiveness of medicine.
The present invention with analgesia, antiinflammatory, invigorating blood circulation act as index, effective site to Radix Lamiophlomidis Rotatae is followed the trail of, Radix Lamiophlomidis Rotatae extract with different total glycosides and general flavone content is tested as medicine, and the result has confirmed that total glycosides and total flavones wherein is the effective ingredient that has analgesia, antiinflammatory in the Radix Lamiophlomidis Rotatae extract, invigorates blood circulation and act on.
The effective site screening and the curative effect of Radix Lamiophlomidis Rotatae extract are proved by following pharmacodynamics test:
Test one: the influence of xylol induced mice ear swelling
Animal: Kunming mouse, male, body weight 22～24g, Mus 10-11 in age week.
Medicine: Radix Lamiophlomidis Rotatae extract 1 (contain total glycosides 0%, contain flavone 0%), Radix Lamiophlomidis Rotatae extract 2 (contain total glycosides 20%, contain flavone 10%), Radix Lamiophlomidis Rotatae extract 3 (contain total glycosides 40%, contain flavone 15%), Radix Lamiophlomidis Rotatae extract 4 (contain total glycosides 40%, contain flavone 20%), Radix Lamiophlomidis Rotatae extract 5 (contain total glycosides 50%, contain flavone 10%), Radix Lamiophlomidis Rotatae extract 6 (contain total glycosides 50%, contain flavone 20%), administration 100mg/Kg respectively.Positive control drug: dexamethasone acetate tablets, Tianjin pharmaceutcal corporation, Ltd produces, specification: 0.75mg/ sheet * 100 slice/bottle, lot number: 000534, face the time spent to be made into the 3mg/ml medicinal liquid with distilled water.
Test method: choose 96 Kunming mouses, male, body weight 22～24g in Mus 10-11 in age week, is divided into 8 groups by body weight, 12 every group at random.Press listed medicine of table 1 and dosage, gastric infusion is 1 time/day respectively, continuous 3 days.60min after the last administration, each treated animal is applied to auricle two sides, a mice left side with dimethylbenzene 50 μ l and causes inflammation, cuts ears after causing scorching 30min, and hammer is got ears same area auricle, weighs, and is calculated as follows swelling degree and inhibitory rate of intumesce.The results are shown in Table 1.
Swelling degree=cause inflammation is picked up the ears, and to cause the inflammation sheet of picking up the ears heavy for sheet weight-non-
Table 1: the influence of xylol induced mice ear swelling (X ± SD)
Group dosage * number of times Mus is counted swelling degree inhibitory rate of intumesce
(g/kg * c) (only) is (%) (mg)
Matched group equal-volume distilled water * 3 12 10.3 ± 2.1-
Dexamethasone tablet group 0.03 * 3 12 7.1 ± 2.4 31.07
Radix Lamiophlomidis Rotatae extract 1 0.1 * 3 12 9.3 ± 2.1 9.71
Radix Lamiophlomidis Rotatae extract 2 0.1 * 3 12 7.6 ± 1.4 26.21
*
Radix Lamiophlomidis Rotatae extract 3 0.1 * 3 12 7.5 ± 2.6 27.18
*
Radix Lamiophlomidis Rotatae extract 4 0.1 * 3 12 7.2 ± 2.3 30.10
*
Radix Lamiophlomidis Rotatae extract 5 0.1 * 3 12 6.8 ± 2.4 33.98
* *
Radix Lamiophlomidis Rotatae extract 6 0.1 * 3 12 6.6 ± 2.1 35.92
* *
Annotate: each administration group and matched group be * * P＜0.01 * * * P＜0.001 relatively
By table 1 result of the test as can be known, the Radix Lamiophlomidis Rotatae extract xylol induced mice ear swelling that contains total glycosides and total flavones has remarkable inhibitory action (P＜0.01).Do not contain the almost not effect of Radix Lamiophlomidis Rotatae extract of total glycosides and total flavones, increasing antiinflammatory action with total glycosides in the extract and content of total flavone has enhancing trend.
Test two: to the bloated test of mice granuloma induced by implantation of cotton pellets
Animal: Kunming mouse, body weight 22-24g.
Medicine: Radix Lamiophlomidis Rotatae extract 1 (contain total glycosides 0%, contain flavone 0%), Radix Lamiophlomidis Rotatae extract 2 (contain total glycosides 20%, contain flavone 10%), Radix Lamiophlomidis Rotatae extract 3 (contain total glycosides 40%, contain flavone 15%), Radix Lamiophlomidis Rotatae extract 4 (contain total glycosides 40%, contain flavone 20%), Radix Lamiophlomidis Rotatae extract 5 (contain total glycosides 50%, contain flavone 10%), Radix Lamiophlomidis Rotatae extract 6 (contain total glycosides 50%, contain flavone 20%), administration 100mg/Kg respectively.Positive control drug: dexamethasone acetate tablets, Tianjin pharmaceutcal corporation, Ltd produces, specification: 0.75mg/ sheet * 100 slice/bottle, lot number: 000534, face the time spent to be made into the 3mg/ml medicinal liquid with distilled water.
Test method: choose 96 Kunming mouses, male, 10 ages in week, body weight 22～24g is divided into 8 groups at random, and each group is the aseptic cotton balls (10mg) of the equal size of subcutaneous implantation in the oxter all, press behind listed medicine of table 2 and the dosage Rhizoma Atractylodis Macrocephalae gastric infusion 1 time/day next day again, continuous 10 days.Each organizes time morning after the last administration, takes off cervical vertebra and puts to death mice, and cotton balls is taken out together with connective tissue on every side, rejects fatty tissue, puts 60 ℃ of oven dry 6h in the baking oven, claims the granuloma induced by implantation of cotton pellets dry weight, and is calculated as follows, and the results are shown in Table 2.
Table 2: test the mice granuloma induced by implantation of cotton pellets is bloated (X ± SD)
Group dosage * natural law Mus is counted granuloma expansibility granuloma suppression ratio
(g/kg * d) (only) (mg/10g body weight) (%)
Matched group isometric(al) * 10 12 55.90 ± 16.44-
Dexamethasone tablet group 0.005 * 10 12 25.68 ± 9.55
*54.06
Radix Lamiophlomidis Rotatae extract 1 0.1 * 10 12 48.24 ± 24.53 13.70
Radix Lamiophlomidis Rotatae extract 2 0.1 * 10 12 37.17 ± 17.21
*33.51
Radix Lamiophlomidis Rotatae extract 3 0.1 * 10 12 35.68 ± 18.04
*36.17
Radix Lamiophlomidis Rotatae extract 4 0.1 * 10 12 27.24 ± 24.02
*51.27
Radix Lamiophlomidis Rotatae extract 5 0.1 * 10 12 24.01 ± 10.17
*57.05
Radix Lamiophlomidis Rotatae extract 6 0.1 * 10 12 23.68 ± 11.25
*57.64
Annotate: each administration group and matched group be * * P＜0.05 * * * P＜0.01 relatively
By table 2 result of the test as can be known, the Radix Lamiophlomidis Rotatae extract that contains total glycosides and total flavones obviously suppresses mice cotton balls granulation hyperplasia swelling (P＜0.01).The Radix Lamiophlomidis Rotatae extract effect that does not contain total glycosides and total flavones is faint, and increasing antiinflammatory action with total glycosides in the extract and content of total flavone has enhancing trend.
Compbined test 1,2 results as can be known, total glycosides and total flavones are the effective ingredient of antiinflammatory action in the Radix Lamiophlomidis Rotatae.
Test three: to the influence of mice acetic acid twisting
Animal: Kunming mouse, body weight 23-25g.
Medicine: Radix Lamiophlomidis Rotatae extract 1 (contain total glycosides 0%, contain flavone 0%), Radix Lamiophlomidis Rotatae extract 2 (contain total glycosides 20%, contain flavone 10%), Radix Lamiophlomidis Rotatae extract 3 (contain total glycosides 40%, contain flavone 15%), Radix Lamiophlomidis Rotatae extract 4 (contain total glycosides 40%, contain flavone 20%), Radix Lamiophlomidis Rotatae extract 5 (contain total glycosides 50%, contain flavone 10%), Radix Lamiophlomidis Rotatae extract 6 (contain total glycosides 50%, contain flavone 20%), administration 100mg/Kg respectively.Positive control drug: tramadol, the Beijing Sihuan Medicine Science and Technology Co., Ltd produces, specification 50mg/ sheet * 10/ box, lot number 0110095.Facing the time spent is made into the 1mg/ml medicinal liquid with distilled water, gastric infusion 10ml/kg.
Test method: choose 96 Kunming mouses, male and female half and half, in Mus 9～10 weeks of age, body weight 23～25g is divided into 8 groups at random by sex and body weight, 10 every group.Press the listed dosage of table 3, experimental group is irritated the stomach Radix Lamiophlomidis Rotatae extract, and the normal control group is irritated stomach isometric(al) distilled water, and positive controls is irritated gastric hydrochloric acid tramadol sheet medicinal liquid before experiment, all be administered once.Behind each treated animal administration 60min, i.p 0.7% acetum 0.2ml/ only.With the abdominal part indent, buttocks is raised, and stretches hind leg for turning round the body index, observes the interior writhing response number of times of injection back 15min and turns round body time of occurrence (incubation period) for the first time, and be calculated as follows the analgesia rate, the results are shown in Table 9.
The influence of table 3 pair mice acetic acid twisting (X ± SD)
The dosage Mus is counted and turns round body number of times analgesia rate incubation period
Group
(g/kg) (only) (second) (inferior/15min) (%)
Matched group-10 208 ± 130 25 ± 13-
Tramadol group 0.01 10 900 ± 0
* *0 ± 0
* *100
Radix Lamiophlomidis Rotatae extract 1 0.1 10 250 ± 199 22 ± 13 12
Radix Lamiophlomidis Rotatae extract 2 0.1 10 560 ± 256 12 ± 13
*52
Radix Lamiophlomidis Rotatae extract 3 0.1 10 650 ± 269 10 ± 13
*60
Radix Lamiophlomidis Rotatae extract 4 0.1 10 760 ± 333 9 ± 7
*64
Radix Lamiophlomidis Rotatae extract 5 0.1 10 830 ± 315 6 ± 9
* *76
Radix Lamiophlomidis Rotatae extract 6 0.1 10 779 ± 278 5 ± 8
* *80
Annotate: each administration group with compare * P＜0.05 * * P＜0.01 * * * P＜0.001
What by table 3 result of the test as can be known, the Radix Lamiophlomidis Rotatae extract that contains total glycosides and total flavones obviously reduced the reaction of acetic acid induced mice stomachache turns round body number of times (P＜0.01).The Radix Lamiophlomidis Rotatae extract effect that does not contain total glycosides and total flavones is faint, and increasing analgesic activity with total glycosides in the extract and content of total flavone has enhancing trend.Total glycosides and total flavones are the effective ingredient of analgesic activity in the Radix Lamiophlomidis Rotatae.
The test four: to senile rat in the thrombotic influence of body
Principle: use unidirectional current continued stimulus common carotid artery 7 minutes, cause the tunica intima damage, activate platelet and blood coagulation system, vascular endothelial cell injury makes PGI simultaneously
2Synthetic and discharge and reduce, cause and form mixed thrombus in the carotid artery vascular gradually.When carotid artery vascular endogenous cause of ill thrombosis and during plug flow, blood vessel far-end temperature bust then.With temperature sensor monitors blood vessel surface variations in temperature, to report to the police automatically by instrument, record begins to temperature bust required time from stimulation, claims duration of congestion OT, i.e. thrombus formation time.Time is short more, represents easy more formation thrombosis; Otherwise the time is long more, represents difficult more formation thrombosis.
Senile rat can form blood stasis body constitution naturally, easily forms thrombus in vivo.Young rat then is difficult for forming thrombosis.So the test in the positive contrast of senile rat, with the negative contrast of young rat.
Material: male rat.Young group Mus 3-4 in the age month is about body weight 250g.Old group Mus 24-27 in the age month, body is given birth to about 500g.Equipment: rat operating-table, operating scissors, ophthalmology tweezer, mosquito forceps, mosquito clamp, rat oral gavage syringe needle, experimental thrombus in vivo form analyzer.Medicine and reagent: Radix Lamiophlomidis Rotatae extract 1 (contain total glycosides 0%, contain flavone 0%), Radix Lamiophlomidis Rotatae extract 2 (contain total glycosides 20%, contain flavone 10%), Radix Lamiophlomidis Rotatae extract 3 (contain total glycosides 40%, contain flavone 15%), Radix Lamiophlomidis Rotatae extract 4 (contain total glycosides 40%, contain flavone 20%), Radix Lamiophlomidis Rotatae extract 5 (contain total glycosides 50%, contain flavone 10%), Radix Lamiophlomidis Rotatae extract 6 (contain total glycosides 50%, contain flavone 20%), administration 50mg/Kg respectively; 20mg/ml pentobarbital sodium solution; Normal saline.
Method: experimental group is only organized rat oral gavage 2ml/ with the 50mg/ml medicine to old every day, 2ml/ of the young rat oral gavage distilled water of positive controls senile rat and negative control group, continuous 14 days.Drug withdrawal fasting on the same day.Lumbar injection 20mg/ml pentobarbital sodium 0.2ml/100g next day (body weight).Cut skin of neck, separate right carotid artery, transfer stimulating electrode at the carotid artery near-end, far-end is transferred the temperature gauge head that connects instrument.Open instrument switch, give 1.5mV galvanic stimulation 7 minutes with damage carotid artery endotheliocyte by stimulating electrode, along with carotid canal intracavity thrombosis forms gradually, blood flow is blocked gradually, and the temperature of carotid artery far-end descends gradually.When blood flow is blocked fully, the temperature bust, instrument is reported to the police, and shows duration of congestion OT, and the OT time is short more, easy more formation thrombosis; The OT time is long more, difficult more formation thrombosis.
Experimental result is carried out statistical test.Concrete numerical value and the results are shown in Table four.
Table four: to senile rat in the thrombotic influence of body
Medicine group example number duration of congestion OT (min)
Radix Lamiophlomidis Rotatae extract 1 12 11.32+1.67
Radix Lamiophlomidis Rotatae extract 2 12 16.13+1.24
* *
Radix Lamiophlomidis Rotatae extract 3 12 17.55+1.86
* *
Radix Lamiophlomidis Rotatae extract 4 12 18.25+1.45
* *
Radix Lamiophlomidis Rotatae extract 5 12 18.89+1.32
* *
Radix Lamiophlomidis Rotatae extract 6 12 19.54+1.58
* *
Old positive controls 12 10.11+1.23
▲ ▲ ▲
Young negative control group 12 18.49+1.75
Annotate:
*P＜0.05
* *Compare with the senile rat group P＜0.01.
▲ ▲ ▲Compare with young rat group P＜0.01
By table four result of the test as can be known, the obviously more young rat of senile rat OT is short, illustrates easily to form thrombosis.But the Radix Lamiophlomidis Rotatae extract significant prolongation OT that contains total glycosides and total flavones, the Radix Lamiophlomidis Rotatae extract that does not contain total glycosides and total flavones can not make OT prolong.Increase the thrombotic effect of inhibition with total glycosides in the extract and content of total flavone enhancing trend is arranged.Total glycosides and total flavones are the effective ingredient of the effect of invigorating blood circulation in the Radix Lamiophlomidis Rotatae.
For investigating the toxic and side effects of Radix Lamiophlomidis Rotatae extract, the inventor has carried out emergency toxicology to Radix Lamiophlomidis Rotatae extract and has investigated.The research method and the result of the test that are adopted are as follows:
Method: improve karber's method (with reference to " herbal pharmacology research methodology " the 113rd page, People's Health Publisher, the 1st edition, Qi Chen chief editor)
Sample: Radix Lamiophlomidis Rotatae extract 4 (contain total glycosides 40%, contain flavone 20%)
Result: LD
50Be 15.59 ± 2.79g/kg, 95% the credible 11.54～18.98g/kg that is limited to.
By above pharmacodynamics and toxicologic study result, can draw to draw a conclusion: Radix Lamiophlomidis Rotatae total glycosides and total flavones are analgesia, antiinflammatory in the Radix Lamiophlomidis Rotatae extract, the effective ingredient of the effect of invigorating blood circulation; And with total glycosides in the extract and content of total flavone increase its analgesia, antiinflammatory, the effect of invigorating blood circulation has enhancing trend.Radix Lamiophlomidis Rotatae saponin LD
50Be 15.59 ± 2.79g/kg, toxicity is lower.
The specific embodiment
The present invention is described in further detail below in conjunction with the specific embodiment.
Embodiment one
Get Radix Lamiophlomidis Rotatae medical material 100kg, add water 800kg, boil and extracted 1 hour, filter, filtrate for later use, medicinal residues add water 700kg again, boil and extracted 1 hour, filter, merge filtrate twice, it is rare extractum of 1.08 that the filtrate after merging is condensed into relative density, this rare extractum is crossed the D101 macroporous adsorbent resin, elder generation's water is eluted to colourless, and this water elution liquid is discarded, and reuse 85% ethanol elution is to colourless, collect this 85% ethanol elution, to be condensed into relative density be 1.11 thick extractum with reclaiming 85% ethanol elution behind the ethanol, with this thick extractum spray drying, obtains containing total glycosides 40%, the Radix Lamiophlomidis Rotatae extract that contains flavone 15%.
Embodiment two
Get Radix Lamiophlomidis Rotatae medical material 100kg, add water 900kg, boil and extracted 2 hours, filter, filtrate for later use, medicinal residues add water 900kg again, boil and extract 1 hour, filter, merge filtrate twice, it is 1.05 that filtrate after merging is concentrated into relative density, and adding ethanol to ethanol content is 40%, leaves standstill 18 hours, get the supernatant, ethanol is reclaimed in this supernatant, and to become relative density be rare extractum of 1.02, and this rare extractum is crossed the D201 macroporous adsorbent resin, extremely colourless with 15% ethanol elution earlier, this 15% ethanol elution is discarded, reuse 60% ethanol elution is collected this 60% ethanol elution to colourless, and to be condensed into relative density be 1.10 thick extractum with reclaiming 60% ethanol elution behind the ethanol, with this thick extractum drying under reduced pressure, the water that adds 5 times of amounts, heating makes dissolving, adds n-butanol extraction again 3 times, each n-butyl alcohol consumption equates with the water yield, combining extraction liquid reclaims the n-butyl alcohol after drying with the extract after merging, and obtains containing total glycosides 50%, the Radix Lamiophlomidis Rotatae extract that contains flavone 20%.
Embodiment three
Get Radix Lamiophlomidis Rotatae medical material 100kg, add water 1000kg, boil and extracted 2 hours, filter, filtrate for later use, medicinal residues add water 900kg again, boil and extracted 1 hour, filter, filtrate for later use, medicinal residues add water 900kg again, boil and extracted 1 hour, filter, merge three times filtrate, it is 1.05 that the filtrate after merging is concentrated into relative density, adding ethanol to ethanol content is 95%, left standstill 24 hours, and got the supernatant, ethanol is reclaimed in this supernatant, and to become relative density be rare extractum of 1.05, this rare extractum is crossed the D201 macroporous adsorbent resin, extremely colourless with 20% ethanol elution earlier, this 20% ethanol elution is discarded, reuse 50% ethanol elution is to colourless, collect this 50% ethanol elution, to be condensed into relative density be 1.09 thick extractum with reclaiming 50% ethanol elution behind the ethanol, with this thick extractum drying under reduced pressure, obtains containing total glycosides 25%, the Radix Lamiophlomidis Rotatae extract that contains flavone 10%.
Embodiment four
Get Radix Lamiophlomidis Rotatae medical material 100kg, add 1% ethanol 700kg, reflux, extract, 1.5 hours, filter, filtrate for later use, medicinal residues add 1% ethanol 700kg again, reflux, extract, 1.5 hours, filter, merge filtrate twice, it is rare extractum of 1.05 that the filtrate after merging is concentrated in relative density, this rare extractum is crossed the AB-8 macroporous adsorbent resin, extremely colourless with 10% ethanol elution earlier, this 10% ethanol elution is discarded, reuse 70% ethanol elution is to colourless, collect this 70% ethanol elution, to be condensed into relative density be 1.10 thick extractum with reclaiming 70% ethanol elution behind the ethanol, with this thick extractum spray drying, obtains total glycosides 35%, the Radix Lamiophlomidis Rotatae extract that contains flavone 15%.
Embodiment five
Get Radix Lamiophlomidis Rotatae medical material 100kg, add 95% ethanol 800kg, reflux, extract, 1.5 hours, filter, filtrate recycling ethanol is not distinguished the flavor of to there being alcohol, it is rare extractum of 1.06 that thin up becomes relative density, this rare extractum is crossed the D301 macroporous adsorbent resin, extremely colourless with 30% ethanol elution earlier, this 30% ethanol elution is discarded, reuse 95% ethanol elution is to colourless, collect this 95% ethanol elution, to be condensed into relative density be 1.35 thick extractum with reclaiming 95% ethanol elution behind the ethanol, with this thick extractum oven dry, obtains containing total glycosides 50%, the Radix Lamiophlomidis Rotatae extract that contains flavone 25%.
Embodiment six
Get Radix Lamiophlomidis Rotatae medical material 100kg, add 50% ethanol 700kg, reflux, extract, 1.5 hours, filter, filtrate for later use, medicinal residues add 50% ethanol 700kg again, and reflux, extract, 1.5 hours filters, merge filtrate twice, it is rare extractum of 1.08 that filtrate recycling ethanol after merging is become relative density, and this rare extractum is crossed the X-5 macroporous adsorbent resin, extremely colourless with 1% ethanol elution earlier, this 1% ethanol elution is discarded, reuse 40% ethanol elution is collected this 40% ethanol elution to colourless, and to be condensed into relative density be 1.13 thick extractum with reclaiming 40% ethanol elution behind the ethanol, with this thick extractum spray drying, the water that adds 5 times of amounts, heating makes dissolving, adds ethyl acetate extraction again 3 times, each ethyl acetate consumption equates with the water yield, combining extraction liquid reclaims the ethyl acetate after drying with the extract after merging, and obtains containing total glycosides 20%, the Radix Lamiophlomidis Rotatae extract that contains flavone 10%.
Embodiment seven
Get Radix Lamiophlomidis Rotatae medical material 100kg, add 70% ethanol 800kg, reflux, extract, 3 hours, filter, filtrate for later use, medicinal residues add 70% ethanol 700kg again, reflux, extract, 3 hours, filtering, merge filtrate twice, is that relative density is rare extractum of 1.08 with the filtrate recycling ethanol after merging, this rare extractum is crossed the D101 macroporous adsorbent resin, elder generation's water is eluted to colourless, and this water elution liquid is discarded, and reuse 80% ethanol elution is to colourless, collect this 80% ethanol elution, with reclaiming that 80% ethanol elution behind the ethanol is condensed into density is 1.15 thick extractum, with this thick extractum lyophilization, obtains containing total glycosides 40%, the Radix Lamiophlomidis Rotatae extract that contains flavone 20%.
Embodiment eight
Get the described Radix Lamiophlomidis Rotatae extract 3kg that contains total glycosides and total flavones, vegetable oil 7kg, mixing is made capsule casing material with gelatin, is pressed into soft capsule, promptly makes the pharmaceutical composition of the soft capsule dosage form that contains 30% Radix Lamiophlomidis Rotatae extract.
Embodiment nine
Get the described Radix Lamiophlomidis Rotatae extract 300g that contains total glycosides and total flavones, be dissolved in the 10000ml water for injection, adopt existing injectable powder lyophilizing preparation technology, promptly make the pharmaceutical composition of the injectable powder type that contains Radix Lamiophlomidis Rotatae extract.
Embodiment ten
Get the described Radix Lamiophlomidis Rotatae extract 300g that contains total glycosides and total flavones, starch 700g, mix homogeneously adopts existing capsules preparation technique, promptly makes the pharmaceutical composition of the capsule formulation that contains Radix Lamiophlomidis Rotatae extract.
Embodiment 11
Get the described Radix Lamiophlomidis Rotatae extract 300g that contains total glycosides and total flavones, adopt existing drop pill preparation technology, promptly make the pharmaceutical composition of the drops that contains Radix Lamiophlomidis Rotatae extract.
Embodiment 12
Get the described Radix Lamiophlomidis Rotatae extract 300g that contains total glycosides and total flavones, sucrose 800g, mix homogeneously adopts existing tablet producing technology, granulates, and sieves, and drying promptly makes the granule forms of pharmaceutical compositions that contains Radix Lamiophlomidis Rotatae extract; Maybe with the granule that makes through further tabletting, drying promptly makes the pharmaceutical composition of the Tabules that contains Radix Lamiophlomidis Rotatae extract.
Embodiment 13
Get the described Radix Lamiophlomidis Rotatae extract 300g that contains total glycosides and total flavones, polyethylene 60g, mix homogeneously adopts existing slow releasing tablet preparation technology, granulates, tabletting, drying promptly makes the pharmaceutical composition of the slow releasing tablet dosage form that contains Radix Lamiophlomidis Rotatae extract.
Embodiment 14
Get the described Radix Lamiophlomidis Rotatae extract 50g that contains total glycosides and total flavones, be dissolved in the 10000ml water for injection, adopt existing process for preparation of injection, promptly make the injection (containing transfusion) of 5mg/ml.
Embodiment 15
Get the described Radix Lamiophlomidis Rotatae extract 300g that contains total glycosides and total flavones, other gets Radix Ginseng total saponins 100g, sucrose 3600g, mix homogeneously adopts existing tablet producing technology, granulates, sieve, drying, tabletting promptly makes the pharmaceutical composition of the Tabules that contains Radix Lamiophlomidis Rotatae extract and Radix Ginseng total saponins.
Claims (10)
1, a kind of Radix Lamiophlomidis Rotatae extract, it is the extract that contains total glycosides and total flavones that extracts from the Chinese medicine Radix Lamiophlomidis Rotatae.
2, Radix Lamiophlomidis Rotatae extract according to claim 1 is characterized in that: the total content of total glycosides and total flavones is more than 50% in the described extract.
3, Radix Lamiophlomidis Rotatae extract according to claim 1 is characterized in that: total glycosides content is more than 20% in the described extract, and general flavone content is more than 10%.
4, Radix Lamiophlomidis Rotatae extract according to claim 3 is characterized in that: total glycosides content is more than 40% in the described extract, and general flavone content is more than 20%.
5, the preparation method of the described Radix Lamiophlomidis Rotatae extract of a kind of claim 1 may further comprise the steps:
(1) preparation Radix Lamiophlomidis Rotatae crude extract: get the raw medicinal material Radix Lamiophlomidis Rotatae or pulverize after Radix Lamiophlomidis Rotatae, extract with decoction and alcohol sedimentation technique or ethanol extraction method, extracting solution, this extracting solution is condensed into rare extractum, promptly get the Radix Lamiophlomidis Rotatae crude extract;
(2) refining: that the Radix Lamiophlomidis Rotatae crude extract that (1) step makes is crossed macroporous adsorbent resin, the low-concentration ethanol eluting of elder generation's water or 1%-30%, the eluent of this water or low-concentration ethanol is discarded need not, remove the impurity that is dissolved in polar solvent, the higher concentration ethanol elution of reuse 40%-95%, collect this higher concentration ethanol elution, the higher concentration ethanol elution drying with behind the recovery ethanol promptly gets the Radix Lamiophlomidis Rotatae extract that contains total glycosides and total flavones.
6, the preparation method of Radix Lamiophlomidis Rotatae extract according to claim 5 is characterized in that: through the Radix Lamiophlomidis Rotatae extract that contains total glycosides and total flavones that (2) one-step refining makes, available n-butyl alcohol or ethyl acetate are further purified processing.
7, the preparation method of Radix Lamiophlomidis Rotatae extract according to claim 5 is characterized in that: the macroporous adsorbent resin described in (2) one-step refining hangs down polar macroporous adsorbent resin in being.
8, the preparation method of Radix Lamiophlomidis Rotatae extract according to claim 5 is characterized in that: the used ethanol of ethanol extraction method is that concentration is the ethanol of 30-95% in (1) step preparation Radix Lamiophlomidis Rotatae crude extract.
9, the application of a kind of claim 1 or 2 or 3 or 4 described Radix Lamiophlomidis Rotatae extracts, described Radix Lamiophlomidis Rotatae extract is used to prepare have analgesia, antiinflammatory, the medicine of the effect of invigorating blood circulation, it is characterized in that: describedly have analgesia, antiinflammatory, contain the above-mentioned Radix Lamiophlomidis Rotatae extract of effective dose in the medicine of the effect of invigorating blood circulation.
10, the application of Radix Lamiophlomidis Rotatae extract according to claim 9 is characterized in that: describedly have analgesia, antiinflammatory, the dosage form of the medicine of the effect of invigorating blood circulation can be any existing pharmaceutical dosage form in injection, transfusion, powder pin, drop pill, tablet, slow releasing tablet, capsule, soft capsule, the granule.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899390B (en) * | 2006-07-18 | 2010-06-02 | 西藏央科生物科技有限公司 | External use analgesic hemostatic preparation and its preparing method |
| CN101940617A (en) * | 2010-09-08 | 2011-01-12 | 西藏奇正藏药股份有限公司 | Medicinal composition with anti-inflammatory and analgesic effects, preparation method and application thereof |
| CN101618069B (en) * | 2009-06-15 | 2012-01-04 | 甘肃独一味生物制药股份有限公司 | Capsule preparation for treating bruise as well as bleeding and easing pain and preparation method and use thereof |
-
2004
- 2004-06-07 CN CN 200410022734 patent/CN1583043A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899390B (en) * | 2006-07-18 | 2010-06-02 | 西藏央科生物科技有限公司 | External use analgesic hemostatic preparation and its preparing method |
| CN101618069B (en) * | 2009-06-15 | 2012-01-04 | 甘肃独一味生物制药股份有限公司 | Capsule preparation for treating bruise as well as bleeding and easing pain and preparation method and use thereof |
| CN101940617A (en) * | 2010-09-08 | 2011-01-12 | 西藏奇正藏药股份有限公司 | Medicinal composition with anti-inflammatory and analgesic effects, preparation method and application thereof |
| CN101940617B (en) * | 2010-09-08 | 2012-08-29 | 西藏奇正藏药股份有限公司 | Medicinal composition with anti-inflammatory and analgesic effects, preparation method and application thereof |
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