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CN1560230A - A kind of fast-rot organic waste bacterial agent and its construction method - Google Patents

A kind of fast-rot organic waste bacterial agent and its construction method Download PDF

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Publication number
CN1560230A
CN1560230A CNA2004100142013A CN200410014201A CN1560230A CN 1560230 A CN1560230 A CN 1560230A CN A2004100142013 A CNA2004100142013 A CN A2004100142013A CN 200410014201 A CN200410014201 A CN 200410014201A CN 1560230 A CN1560230 A CN 1560230A
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China
Prior art keywords
composite bacteria
high temperature
bacteria
lignocellulose
organic waste
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哲 朴
朴哲
杨林章
殷士学
胡健
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Yangzhou University
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Yangzhou University
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

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  • Fertilizers (AREA)

Abstract

本发明公开了垃圾处理技术领域内的一种有机废弃物快腐菌剂及其构建方法，所述快腐菌剂包括细菌、放线菌、真菌等不同类群的微生物，不同微生物间具有良好的协同作用。本发明所述菌剂直接以复合菌的形式分离筛选木质纤维素分解菌群而得。所述快腐菌剂不仅对作物秸秆、畜禽粪便、生活垃圾等有机废弃物具有高效稳定的降解机能，而且对不同环境的适应性及抗污染能力强，在有机废弃物资源化再生利用及环保等方面具有广阔的应用前景。The invention discloses a fast-rotting microbial agent for organic waste in the technical field of garbage treatment and a construction method thereof. The fast-rotating microbial agent includes microorganisms of different groups such as bacteria, actinomycetes, and fungi, and different microorganisms have good synergy. The microbial agent of the present invention is directly obtained by separating and screening lignocellulosic decomposing bacterial groups in the form of complex bacteria. The fast-rot fungus not only has an efficient and stable degradation function for organic waste such as crop straw, livestock and poultry manure, domestic garbage, etc., but also has strong adaptability to different environments and strong anti-pollution ability. Environmental protection and other aspects have broad application prospects.

Description

A kind of organic waste quick puterfaction bacteria agent and construction process thereof
Technical field
The present invention relates to a kind of quick degraded composite fungus agent and construction process thereof of organic waste, belong to the organic waste processing technology field.
Background technology
Along with The development in society and economy, more and more at the organic waste that the various fields of society produce.No matter be developed country or developing country, all face the serious threat that waste causes disaster inevitably.Organic waste has become big public hazards of society, and its contaminate environment is engulfed the arable land, the harm people's physical and mental health.How to make that organic waste is innoxious, resource utilization, minimizing, turn waste into wealth, problem demanding prompt solution has become international.Biological degradation is one of effective way of the present reclaiming organic waste regeneration of generally confessing, makes at fertilizer, and aspects such as feed, sugaring, fuel industry and environmental protection have broad application prospects.
At present, the degradable solid organic castoff of each field generation of society is mainly formed with the lignocellulose material.Wherein, Mierocrystalline cellulose accounts for 40%, and hemicellulose accounts for 20%～30%, and xylogen accounts for 20%～30%.Therefore, constantly select the microorganism with efficient stable ligocellulose degradation function, be active research field the most in the recycling treatment process of organic waste always.Under the state of nature, all close-connected characteristic of lignocellulose material, this type of material demand of thoroughly degrading depends on the fellowship of multiple microorganisms such as Mierocrystalline cellulose, hemicellulose and xylogen decomposer and could realize down.Therefore, in recent years in the biological degradation field of lignocellulose mix bacterium agent begun to cause people's great attention.
So far, existing a lot of report about organic waste degradation mix bacterium agent aspect.But existing mix bacterium agent at first adopts traditional pure separation method screening major function microorganism, two or more pure bacterial strain is made up artificially make up and preparation.And generally adopt the method for liquid fermenting to prepare microbial inoculum, improved production cost inevitably.Several problems that existing biological degradation microbial inoculum mainly exists:
1, the prior biological degradation bacterial agent is confined to separable microorganism, and the separable microorganism of occurring in nature is less than 10%, and traditional pure separation method has limited artificially and has had the efficient degradation function under field conditions (factors) but the utilization of inseparable microorganism, and it is unilateral therefore only paying attention to separable microorganism;
2, will thoroughly degrade the under field conditions (factors) fellowship of the multiple microorganism of certain material demand could be realized down, and adopt traditional pure isolating method to carry out in the screening process, original micro-flora is destroyed under the natural condition, be easy to miss that to produce enzyme performance more weak relatively or do not produce enzyme, but have good synergistic microorganism;
3, adopt microbial inoculum that pure strain makes in actual production and application process, problems such as the ubiquity contamination resistance is poor,, production cost height narrower to strict, degraded function instability, the practical ranges of fermentation condition.
Summary of the invention
One of purpose of the present invention provides a kind of organic waste quick puterfaction bacteria agent, and this microbial inoculum contamination resistance is strong, have good synergy between different microorganisms, the wide ranges of, the pH that be fit to tight to the requirement of oxygen and temperature and the lignocellulosic material that is difficult to degrade is had the degraded function of efficient stable.
One of purpose of the present invention is achieved in that a kind of organic waste quick puterfaction bacteria agent, described microbial inoculum is made of jointly the suitable composite flora of having a liking for warm microorganism and thermophilic microorganism of pH scope between 4.0～10.0, contain bacterium, actinomycetes and the fungi that can produce the lignocellulose enzyme in the described microbial inoculum, bacterium mainly is made up of genus bacillus, actinomycetes mainly are made up of streptomycete, fungi is mainly formed with aspergillus by wood is mould, contains bacterium 10 in the microbial inoculum of every dry weight 1 gram 8～10 10Individual, actinomycetes 10 6～10 8Individual, fungi 10 5～10 7Individual.
Quick puterfaction bacteria agent of the present invention has good Degradation to organic waste, synergetic property is good between different microorganisms, under the different fermentations condition, all show very strong synergy and contamination resistance, applicable to the degradation treatment of organic waste, in treating processes, can also produce fertilizer.
Two of purpose of the present invention provides a kind of construction process of organic waste quick puterfaction bacteria agent.
Two of purpose of the present invention can realize by two kinds of methods:
One of method may further comprise the steps:
A) screening of high temperature composite bacteria: from be rich in lignocellulose decomposer environment, take a sample, with the lignocellulose is sole carbon source, under 50～60 ℃, leave standstill enrichment culture, continuous passage to the micro-flora and the degraded function of enrichment culture thing stablized under identical fermentation condition, is the high temperature composite bacteria;
B) screening of warm composite bacteria in: from be rich in lignocellulose decomposer environment, take a sample, with the lignocellulose is sole carbon source, on 25～35 ℃ of temperature, 150～200rpm/min shaking table, carry out enrichment culture, continuous passage to the micro-flora and the degraded function of enrichment culture thing stablized under identical fermentation condition, as middle temperature composite bacteria;
C) the middle temperature composite bacteria that is obtained is mixed by identical bacterium number with the high temperature composite bacteria, and be transferred to the solid fermentation substratum with 2～5% inoculum sizes, in 25～35 ℃, carried out shallow tray fermentation 5～7 days under gentle 50～60 ℃ of high temperature respectively, solid medium thickness is 5～10cm, carried out turning every 2 days, two kinds of cultures after the middle gentle high-temperature cultivation are dried down at 40～50 ℃ respectively, pulverize, cross 10～20 mesh sieve, warm solid koji and high-temp solid song in obtaining, the middle temperature solid koji and 70%～30% high-temp solid Qu Jinhang of mass content 30%～70% are mixed, promptly obtain described a kind of organic waste quick puterfaction bacteria agent.
Two of method may further comprise the steps:
A) screening of warm composite bacteria in: from be rich in lignocellulose decomposer environment, take a sample, with the lignocellulose is sole carbon source, on 25～35 ℃ of temperature, 150～200rpm/min shaking table, carry out enrichment culture, continuous passage to the micro-flora and the degraded function of enrichment culture thing stablized under identical fermentation condition, as middle temperature composite bacteria;
B) with the middle temperature composite bacteria that obtained by 2～5% inoculum sizes switching solid fermentation substratum, carried out shallow tray fermentation 5～7 days under 25～35 ℃, the substratum gauge control was carried out turning at 5～10cm every 2 days, dry down, pulverize the solid koji of warm compound system in promptly becoming for 40～50 ℃;
C) screening of high temperature composite bacteria: from be rich in lignocellulose decomposer environment, take a sample, with the lignocellulose is sole carbon source, under 50～60 ℃, leave standstill enrichment culture, continuous passage to the micro-flora and the degraded function of enrichment culture thing stablized under identical fermentation condition, is the high temperature composite bacteria;
D) with the high temperature composite bacteria that is obtained, be inoculated into liquid fermentation medium in 2～5% ratios, 50～60 ℃ of following static fermentations 3～5 days are the fermented liquid of high temperature bacterium;
E) fermented liquid of high temperature bacterium is adsorbed onto with identical bacterium number in the solid koji of warm compound system, 40～50 ℃ of oven dry down, promptly become described a kind of organic waste quick puterfaction bacteria agent.
A kind of organic waste quick puterfaction bacteria agent of the present invention has following characteristic:
Isolate the different types of microorganism of 14 strains (seeing Table 1) in A, the microbial inoculum of the present invention altogether, be respectively 6 bacillus, 4 strain actinomycetes, 4 fungal strains, wherein bacterium and actinomycetes are made up of genus bacillus and streptomycete respectively, and fungi is formed with aspergillus by wood is mould.As seen this composite flora is made of jointly different monoid microorganisms such as bacterium, actinomycetes, fungies.
The existing mesophile of composite flora (30 ℃) in B, the microbial inoculum of the present invention has thermophile bacteria (55 ℃) again, and mesophile kind and quantity all are higher than thermophile bacteria (seeing Table 1).Number be the obligate high temperature bacterium except strain actinomycetes (PC2-f4) in this composite flora, thermophilic bacteria and fungi all belong to facultative high temperature bacterium (as YC2-x1～x4 and YC2-z1～z2).
The middle temperature of C, quick puterfaction bacteria agent of the present invention (30 ℃) microbe population is respectively bacterium 10 10Individual/g (dry sample), actinomycetes 10 8Individual/g (dry sample), fungi 10 7Individual/g (dry sample); And high temperature (55 ℃) microbe population is respectively, bacterium 10 8Individual/g (dry sample), actinomycetes 10 6Individual/g (dry sample), fungi 10 5Individual/g (dry sample) (seeing Table 1).
YUP1 micro-flora under the different culture temperature of table 1
Microbe species ??????????????30℃ ?????????????????55℃
Kind Quantity Kind Quantity
Bacterium ??YC1-x1～x6 ??2.7×10 10 ????YC1-x1～x4 ????3.5×10 8
Actinomycetes ??YC2-f1～f3 ??7.2×10 8 ????YC2-f4 ????4.2×10 6
Fungi ??YC3-z1～z4 ??2.1×10 7 ????YC3-z1～z2 ????3.6×10 5
Before mixing, the degradation efficiency of the quick puterfaction bacteria agent that is mixed and made into by high temperature composite bacteria and middle temperature composite bacteria under temperature or the 55 ℃ of hot conditionss among the D, 30 ℃ is significantly improved (seeing Table 2).
The synergistic blend of YPC1 under the table 2 solid fermentation condition
Handle Enzyme work/U Mode of appearance Smell Rate of weight loss/%
??30℃ ??55℃ ??30℃ ??55℃ ??30℃ ??55℃ ??30℃ ??55℃
The high temperature composite bacteria ??96.0 ??537.6 Primary colors Vandyke brown Do not have Become thoroughly decomposed ??13.6 ??45.2
In warm composite bacteria ??320.4 ??273.5 Greyish white Light brown Musty Become thoroughly decomposed ??36.8 ??42.7
Quick puterfaction bacteria agent ??408.2 ??697.9 Greyish white Vandyke brown Musty Dense becoming thoroughly decomposed ??46.5 ??55.6
E, 30 ℃ of suitable water ratio of quick puterfaction bacteria agent of the present invention are 55%～70%, and wherein optimum moisture percentage is 65% (seeing Table 3); Suitable water ratio is 60～80% under 55 ℃ the high temperature, and wherein optimum moisture percentage is 70%.
Table 3 different water cut rate is to the influence of degradation effect
Culture temperature/℃ Water ratio/% Enzyme work/U.ml -1 Rate of weight loss/%
??30 ????50 ????83.06 ????17.75
????60 ????100.13 ????29.11
????65 ????142.38 ????35.66
????70 ????115.20 ????31.30
????80 ????0.02 ????7.5
??55 ????50 ????237.88 ????25.68
????60 ????230.04 ????33.71
????65 ????278.50 ????36.72
????70 ????250.79 ????39.85
????80 ????203.13 ????34.54
F, a kind of organic waste quick puterfaction bacteria agent of the present invention are the mixed fermentation products by high temperature composite bacteria and middle temperature composite bacteria, the temperature range that is fit to is wide, all show stable degraded function (seeing Table 4) in 20～65 ℃ of temperature ranges, wherein optimum temperuture is 55 ℃.
The function of degrading under table 4 condition of different temperatures (fermenting 5 days)
Leavening temperature/℃ ??25 ??30 ??45 ????55 ??65
Rate of weight loss/% ??40.2 ??45.3 ??48.7 ????56.5 ??46.8
G, quick puterfaction bacteria agent of the present invention, suitable pH scope are 4～10, and wherein the suitableeest pH is 6.0～8.0 (seeing Table 5).
Filter paper disintegration situation under the different initial pH conditions of table 5
????pH 1 day 2 days 3 days 4 days 5 days 6 days 7 days 8 days
????4.0 ??～ ??～ ??～ ??～ ??～ ????+ ????++ ??0
????5.0 ??～ ??+ ??+++ ??0 ??0 ????0 ????0 ??0
????6.0 ??～ ??+ ??+++ ??0 ??0 ????0 ????0 ??0
????7.0 ??+ ??+++ ??0 ??0 ??0 ????0 ????0 ??0
????8.0 ??+ ??+++ ??0 ??0 ??0 ????0 ????0 ??0
????9.0 ??～ ??～ ??++ ??++ ??0 ????0 ????0 ??0
????10.0 ??～ ??～ ??～ ??～ ??～ ????++ ????++ ??0
～: also do not begin to decompose; +: the expression decomposition intensity; 0: all decomposed
Embodiment
Embodiment 1
By making During High-Temperature Composting method enrichment lignocellulose decomposer under field conditions (factors):
1, the making of During High-Temperature Composting:
Take by weighing the fresh crop material of 500kg (as rice straw, straw etc.), add the fresh feces of livestock and poultry of 50kg (chicken manure, pig manure, cow dung etc.), regulating water ratio is 65%, puddles equably, piles mountain type heap body.Be warming up to hot stage and carried out turning every 2 days in the making processes of compost, and regulate water ratio simultaneously, heap temperature is controlled in 65 ℃ as far as possible, water ratio keeps 60%～65%; Be cooled to the spontaneous fermentation of after-ripening stage.
2, the screening of high temperature composite bacteria
A, sampling: from the hot stage compost sample, take a sample;
The liquid preparation that B, soil leach: matured compost or fertile vegetable garden soil 200g add the 1000mL tap water, boil 1h, filter, and sterilization is preserved stand-by;
The preparation of C, liquid fermentation medium A (W/%): peptone 0.5, fresh rice straw powder 1, NaCl 0.5, CaCO 30.2, yeast powder 0.1, soil extract 10,0.5 * 6cm Xinhua filter paper paper slip;
The screening of D, high temperature composite bacteria: take by weighing the compost sample of the above-mentioned hot stage of 5g, add in the 250ml triangular flask of 100mL sterilized water, under 180rpm, shake 30min.Be added among the test tube liquid fermentation medium A in 5% (v/v) ratio that (1.8 * 18cm), the height of substratum keeps about 6～10cm, 55 ℃ of static enrichment culture down.When filter paper will disintegration, be transferred to fresh liquid fermentation medium A by 5% inoculum size, under identical culture condition continuous passage to the enrichment culture thing micro-flora and to produce enzyme performance stable; This compound system is as the high temperature composite bacteria.
3, the screening of middle temperature composite bacteria
A, sampling: from the During High-Temperature Composting sample in after-ripening stage, take a sample;
The preparation of B, liquid fermentation medium B (W/%): fresh rice straw powder 1.0, wheat bran 0.5, peptone 0.05, urea 0.03, KH 2PO 40.4, K 2HPO 40.3, (NH 4) 2SO 47H 2O0.03, Tween-80 0.2, FeSO 47H 2O 0.05, MnSO 4H 2O 0.16, ZnSO 47H 2O 0.14, CaCl 2Trace (2mg), pH6.0;
The screening of C, middle temperature composite bacteria: the sample of compost being made the after-ripening stage adds by 2～5% solid-to-liquid ratios and is equipped with in the 500ml triangular flask of 200ml liquid fermentation medium B, on 30 ℃, 180rpm/min shaking table, carries out enrichment culture 5～7 days.This enriched substance as seed, is transferred in the fresh substratum by 5% inoculum size, and successive goes down to posterity down under identical fermentation condition, and the micro-flora in nutrient solution tends towards stability substantially, and this enriched substance is middle temperature composite bacteria.
4, the preparation of quick puterfaction bacteria agent
The preparation of A solid fermentation substratum (W/%): be cut into the fresh rice straw 77% of 2～5cm, wheat bran 20%, (NH 4) 2SO 42%, urea 0.5%, KH 2PO 40.25%, MgSO 47H 2O 0.25%, and pH is a nature, water ratio 65%; Sterilization 1h;
B, will screen good middle temperature composite bacteria and high temperature composite bacteria and mix in 1: 1 ratio, be transferred to above-mentioned solid fermentation substratum by 2～5% inoculum sizes, under 30 ℃ and 55 ℃, carried out shallow tray fermentation 5～7 days respectively, the substratum gauge control is at 5～10cm, carried out turning every 2 days, dry down, pulverize for 40～50 ℃, cross 10～20 mesh sieve, middle temperature solid koji after will sieving at last (30 ℃ of bottom fermentations) and high-temp solid song (55 ℃ of bottom fermentations) mix in 1: 1 ratio of mass ratio, promptly become microbial inoculum of the present invention.
Embodiment 2
1, the screening of high temperature composite bacteria
A, sampling: from embodiment 1, take a sample in the hot stage compost sample;
The liquid preparation that B, soil leach: matured compost or fertile vegetable garden soil 200g add the 1000mL tap water, boil 1h, filter, and sterilization is preserved stand-by;
The preparation of C, liquid fermentation medium A (W/%): peptone 0.5, fresh rice straw powder 1, NaCl 0.5, CaCO 30.2, yeast powder 0.1, soil extract 10,0.5 * 6cm Xinhua filter paper paper slip;
The screening of D, high temperature composite bacteria: take by weighing the compost sample of the above-mentioned hot stage of 5g, add in the 250ml triangular flask of 100mL sterilized water, under 200rpm, shake 30min.Be added among the test tube liquid fermentation medium A in 5% (v/v) ratio that (1.8 * 18cm), the height of substratum keeps about 6～10cm, 60 ℃ of static enrichment culture down.When filter paper will disintegration, be transferred to fresh liquid fermentation medium A by 5% inoculum size, under identical culture condition continuous passage to the enrichment culture thing micro-flora and to produce enzyme performance stable; This compound system is as the high temperature composite bacteria.
2, the screening of middle temperature composite bacteria
A, sampling: from embodiment 1, take a sample in the During High-Temperature Composting sample in after-ripening stage;
The preparation of B, liquid fermentation medium B (W/%): fresh rice straw powder 1.0, wheat bran 0.5, peptone 0.05, urea 0.03, KH 2PO 40.4, K 2HPO 40.3, (NH 4) 2SO 47H 2O0.03, Tween-80 0.2, FeSO 47H 2O 0.05, MnSO 4H 2O 0.16, ZnSO 47H 2O 0.14, CaCl2 trace (2mg), pH6.0;
The screening of C, middle temperature composite bacteria: the sample of compost being made the after-ripening stage adds by 2～5% solid-to-liquid ratios and is equipped with in the 500ml triangular flask of 200ml liquid fermentation medium B, on 35 ℃, 200rpm/min shaking table, carries out enrichment culture 5～7 days.This enriched substance as seed, is transferred in the fresh substratum by 5% inoculum size, and successive goes down to posterity down under identical fermentation condition, and the micro-flora in nutrient solution tends towards stability substantially, and this enriched substance is middle temperature composite bacteria.
3, the preparation of quick puterfaction bacteria agent
The preparation of A, solid fermentation substratum (W/%): be cut into the fresh rice straw 77% of 2～5cm, wheat bran 20%, (NH 4) 2SO 42%, urea 0.5%, KH 2PO 40.25%, MgSO 47H 2O 0.25%, and pH is a nature, water ratio 65%; Sterilization 1h;
B, will screen good middle temperature composite bacteria and high temperature composite bacteria and mix in 1: 1 ratio, be transferred to above-mentioned solid fermentation substratum by 2～5% inoculum sizes, under 35 ℃ and 60 ℃, carried out shallow tray fermentation 5～7 days respectively, the substratum gauge control is at 10cm, carried out turning every 2 days, dry down, pulverize for 40～50 ℃, cross 10～20 mesh sieve, middle temperature solid koji after will sieving at last (35 ℃ of bottom fermentations) and high-temp solid song (60 ℃ of bottom fermentations) mix in 7: 3 ratios of mass ratio, promptly become microbial inoculum of the present invention.
Embodiment 3
1, the screening of high temperature composite bacteria
A, sampling: from hot stage compost sample described in the embodiment 1, take a sample;
The liquid preparation that B, soil leach: matured compost or fertile vegetable garden soil 200g add the 1000mL tap water, boil 1h, filter, and sterilization is preserved stand-by;
The preparation of C, liquid fermentation medium A (W/%): peptone 0.5, fresh rice straw powder 1, NaCl 0.5, CaCO 30.2, yeast powder 0.1, soil extract 10,0.5 * 6cm Xinhua filter paper paper slip;
The screening of D, high temperature composite bacteria: take by weighing the compost sample of the above-mentioned hot stage of 5g, add in the 250ml triangular flask of 100mL sterilized water, under 150rpm, shake 30min.Be added among the test tube liquid fermentation medium A in 5% (v/v) ratio that (1.8 * 18cm), the height of substratum keeps about 6～10cm, 50 ℃ of static enrichment culture down.When filter paper will disintegration, be transferred to fresh liquid fermentation medium A by 5% inoculum size, under identical culture condition continuous passage to the enrichment culture thing micro-flora and to produce enzyme performance stable; This compound system is as the high temperature composite bacteria.
2, the screening of middle temperature composite bacteria
A, sampling: from embodiment 1, take a sample in the During High-Temperature Composting sample in after-ripening stage;
The preparation of B, liquid fermentation medium B (W/%): fresh rice straw powder 1.0, wheat bran 0.5, peptone 0.05, urea 0.03, KH 2PO 40.4, K 2HPO 40.3, (NH 4) 2SO 47H 2O0.03, Tween-80 0.2, FeSO 47H 2O 0.05, MnSO 4H 2O 0.16, ZnSO 47H 2O 0.14, CaCl2 trace (2mg), pH6.0;
C, middle temperature composite bacteria screening: the sample of compost being made the after-ripening stage adds by 2～5% solid-to-liquid ratios and is equipped with in the 500ml triangular flask of 200ml liquid fermentation medium B, on 25 ℃, 150rpm/min shaking table, carries out enrichment culture 5～7 days.This enriched substance as seed, is transferred in the fresh substratum by 5% inoculum size, and successive goes down to posterity down under identical fermentation condition, and the micro-flora in nutrient solution tends towards stability substantially, and this enriched substance is middle temperature composite bacteria.
3, the preparation of quick puterfaction bacteria agent
A, prepare the solid fermentation substratum in ratio described in embodiment 1 or 2;
B, will screen good middle temperature composite bacteria and high temperature composite bacteria and mix in 1: 1 ratio, be transferred to above-mentioned solid fermentation substratum by 2～5% inoculum sizes, under 25 ℃ and 50 ℃, carried out shallow tray fermentation 5～7 days respectively, the substratum gauge control is at 5cm, carried out turning every 2 days, dry down, pulverize for 40～50 ℃, cross 10～20 mesh sieve, middle temperature solid koji after will sieving at last (25 ℃ of bottom fermentations) and high-temp solid song (50 ℃ of bottom fermentations) mix in 3: 7 ratios of mass ratio, promptly become microbial inoculum of the present invention.
Embodiment 4
The middle temperature composite bacteria and the high temperature composite bacteria that obtain among the embodiment 1 are fermented under solid-state and liquid condition respectively, at last liquid bacterial agent is adsorbed onto in the solid fungicide; Concrete steps are:
A, middle temperature composite bacteria were inoculated into the solid fermentation substratum in 2% ratio, carry out shallow tray fermentation 5～7 days under 25 ℃, and the substratum gauge control was carried out turning at 5cm every 2 days, dried down, pulverized for 40～50 ℃, crossed 10～20 mesh sieve, and be stand-by;
B, the high temperature composite bacteria is transferred to described in the liquid embodiment 1 among the fermention medium A by 2% inoculum size, 50 ℃ of following static fermentations 3～5 days, the filter paper to the substratum is fully by disintegration.
C, the high temperature composite bacteria is adsorbed onto with identical bacterium number in the solid koji of warm composite bacteria, 40～50 ℃ of oven dry down, promptly become microbial inoculum of the present invention.
Embodiment 5
The middle temperature composite bacteria and the high temperature composite bacteria that obtain among the embodiment 1 are fermented under solid-state and liquid condition respectively, at last liquid bacterial agent is adsorbed onto in the solid fungicide; Concrete steps are:
A, middle temperature composite bacteria are inoculated into the solid fermentation substratum described in the foregoing description in 5% ratio, carry out shallow tray fermentation 5～7 days under 35 ℃, and the substratum gauge control is at 10cm, carried out turning every 2 days, dry down, pulverize for 40～50 ℃, cross 10～20 mesh sieve, stand-by;
B, the high temperature composite bacteria is transferred to described in the liquid embodiment 1 among the fermention medium A by 5% inoculum size, 60 ℃ of following static fermentations 3～5 days, the filter paper to the substratum is fully by disintegration.
C, the high temperature composite bacteria is adsorbed onto with identical bacterium number in the solid koji of warm composite bacteria, 40～50 ℃ of oven dry down, promptly become microbial inoculum of the present invention.
Embodiment 6
Take by weighing the fresh crop material of 1000kg (as rice straw, straw etc.), add the fresh feces of livestock and poultry (chicken manure of 50～100kg, pig manure, cow dung etc.), 1kg is by composite fungus agent that the foregoing description obtained, regulating water ratio is 65%, puddle equably, (pile the elongated heap body of high 1.2m, wide 1.5m in 1.2m * 1.5m * 20m), ferment at the elongated fermenter.Be warming up to hot stage and carried out turning every 2 days and regulate water ratio simultaneously in the making processes of compost, heap temperature is controlled in 65 ℃ as far as possible, water ratio keeps 60%～65%; Be cooled to the spontaneous fermentation of after-ripening stage, fermenting can be made into the fine During High-Temperature Composting in 15 days, and the organism in the raw material has obtained degraded.
Embodiment 7
Take by weighing the fresh crop material of 1000kg (as rice straw, straw etc.), add 2～5kg by composite fungus agent, 20～30kg urea or sulphur ammonium, 50kg matured compost that the foregoing description obtained, regulating water ratio is 65%, puddle equably, (pile the elongated heap body of high 1.2m, wide 1.5m in 1.2m * 1.5m * 20m), ferment at the elongated fermenter.Be warming up to hot stage and regulate water ratio in the making processes of compost when 2 days carry out turning, heap temperature is controlled in 65 ℃ as far as possible, water ratio keeps 60%～65%; Be cooled to the spontaneous fermentation of after-ripening stage, ferment and can reach the purpose of becoming thoroughly decomposed after 15 days, the lignocellulose in the stalk has obtained degraded.

Claims (3)

1, a kind of organic waste quick puterfaction bacteria agent, it is characterized in that: described microbial inoculum is made of jointly the suitable composite flora of having a liking for warm microorganism and thermophilic microorganism of pH scope between 4.0～10.0, contain bacterium, actinomycetes and the fungi that can produce the lignocellulose enzyme in the described microbial inoculum, bacterium mainly is made up of genus bacillus, actinomycetes mainly are made up of streptomycete, fungi is mainly formed with aspergillus by wood is mould, contains bacterium 10 in the microbial inoculum of every dry weight 1 gram 8～10 10Individual, actinomycetes 10 6～10 8Individual, fungi 10 5～10 7Individual.
2, a kind of organic waste quick puterfaction bacteria agent according to claim 1, its construction process comprises the steps:
A) screening of high temperature composite bacteria: from be rich in lignocellulose decomposer environment, take a sample, with the lignocellulose is sole carbon source, under 50～60 ℃, leave standstill enrichment culture, continuous passage to the micro-flora and the degraded function of enrichment culture thing stablized under identical fermentation condition, is the high temperature composite bacteria;
B) screening of warm composite bacteria in: from be rich in lignocellulose decomposer environment, take a sample, with the lignocellulose is sole carbon source, on 25～35 ℃ of temperature, 150～200rpm/min shaking table, carry out enrichment culture, continuous passage to the micro-flora and the degraded function of enrichment culture thing stablized under identical fermentation condition, as middle temperature composite bacteria;
C) the middle temperature composite bacteria that is obtained is mixed by identical bacterium number with the high temperature composite bacteria, and be transferred to the solid fermentation substratum with 2～5% inoculum sizes, in 25～35 ℃, carried out shallow tray fermentation 5～7 days under gentle 50～60 ℃ of high temperature respectively, solid medium thickness is 5～10cm, carried out turning every 2 days, two kinds of cultures after the middle gentle high-temperature cultivation are dried down at 40～50 ℃ respectively, pulverize, cross 10～20 mesh sieve, warm solid koji and high-temp solid song in obtaining, the middle temperature solid koji and 70%～30% high-temp solid Qu Jinhang of mass content 30%～70% are mixed, and the mixture that is obtained is described a kind of organic waste quick puterfaction bacteria agent.
3, a kind of organic waste quick puterfaction bacteria agent according to claim 1, its construction process comprises the steps:
A) screening of warm composite bacteria in: from be rich in lignocellulose decomposer environment, take a sample, with the lignocellulose is sole carbon source, on 25～35 ℃ of temperature, 150～200rpm/min shaking table, carry out enrichment culture, continuous passage to the micro-flora and the degraded function of enrichment culture thing stablized under identical fermentation condition, as middle temperature composite bacteria;
B) with the middle temperature composite bacteria that obtained by 2～5% inoculum sizes switching solid fermentation substratum, carried out shallow tray fermentation 5～7 days under 25～35 ℃, the substratum gauge control was carried out turning at 5～10cm every 2 days, dry down, pulverize the solid koji of warm compound system in promptly becoming for 40～50 ℃;
C) screening of high temperature composite bacteria: from be rich in lignocellulose decomposer environment, take a sample, with the lignocellulose is sole carbon source, under 50～60 ℃, leave standstill enrichment culture, continuous passage to the micro-flora and the degraded function of enrichment culture thing stablized under identical fermentation condition, is the high temperature composite bacteria;
D) with the high temperature composite bacteria that is obtained, be inoculated into liquid fermentation medium in 2～5% ratios, 50～60 ℃ of following static fermentations 3～5 days are the fermented liquid of high temperature bacterium;
E) fermented liquid of high temperature bacterium is adsorbed onto with identical bacterium number in the solid koji of warm compound system, 40～50 ℃ of oven dry down, promptly become described a kind of organic waste quick puterfaction bacteria agent.
CNA2004100142013A 2004-03-01 2004-03-01 A kind of fast-rot organic waste bacterial agent and its construction method Pending CN1560230A (en)

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CN100408673C (en) * 2006-09-04 2008-08-06 利耀铭 Organic microbial composite and use
CN101497870B (en) * 2009-03-02 2011-04-13 北京市京圃园生物工程有限公司 Microbial complex bacterial agent and use thereof
CN102061270A (en) * 2010-04-20 2011-05-18 怀化学院 Streptomyces thermocarboxydus strain and cultivating method thereof
CN102234616A (en) * 2010-04-28 2011-11-09 中国科学院沈阳应用生态研究所 Corrosion promoting agent for straw, its preparation method and application
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100408673C (en) * 2006-09-04 2008-08-06 利耀铭 Organic microbial composite and use
CN101497870B (en) * 2009-03-02 2011-04-13 北京市京圃园生物工程有限公司 Microbial complex bacterial agent and use thereof
CN102061270A (en) * 2010-04-20 2011-05-18 怀化学院 Streptomyces thermocarboxydus strain and cultivating method thereof
CN102234616B (en) * 2010-04-28 2013-01-30 中国科学院沈阳应用生态研究所 A straw rotting agent and its preparation and application
CN102234616A (en) * 2010-04-28 2011-11-09 中国科学院沈阳应用生态研究所 Corrosion promoting agent for straw, its preparation method and application
CN102321992A (en) * 2011-06-22 2012-01-18 韩保新 Agent for recycling straws by using biologically cold fermentation method and process for producing plant fibers by using straws with biologically cold fermentation method
CN102321992B (en) * 2011-06-22 2012-07-04 韩保新 Agent for recycling straws by using biologically cold fermentation method and process for producing plant fibers by using straws with biologically cold fermentation method
CN103980906A (en) * 2014-05-14 2014-08-13 扬州大学 Biological-reinforced carbon conditioning agent and method of reducing soluble salt content in secondary salinized soil using same
CN103980906B (en) * 2014-05-14 2017-10-17 扬州大学 A kind of biological reinforced carbon conditioning agent and the method using its reduction secondary salinization soil soluble-salt content
CN104725087A (en) * 2015-01-30 2015-06-24 上海胜维有机肥有限公司 Environment-friendly and harmless novel technology for producing organic fertilizers by utilizing agricultural wastes
CN104651277A (en) * 2015-02-09 2015-05-27 福建三炬生物科技股份有限公司 Straw composting bacterium agent as well as preparation method and application thereof
CN104651277B (en) * 2015-02-09 2017-10-20 福建三炬生物科技股份有限公司 A kind of straw decomposing microbial inoculum and its preparation method and application
CN106636234A (en) * 2015-10-29 2017-05-10 清华大学 Method for producing microbial oil through combination of bacterial flora and oleaginous microorganisms
CN106636234B (en) * 2015-10-29 2021-11-16 清华大学 Method for producing microbial oil by combining flora and oil-producing microorganisms

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