CN1537465A - Functional food contg. ewe placenta, and its prepn. method - Google Patents
Functional food contg. ewe placenta, and its prepn. method Download PDFInfo
- Publication number
- CN1537465A CN1537465A CNA2003101048546A CN200310104854A CN1537465A CN 1537465 A CN1537465 A CN 1537465A CN A2003101048546 A CNA2003101048546 A CN A2003101048546A CN 200310104854 A CN200310104854 A CN 200310104854A CN 1537465 A CN1537465 A CN 1537465A
- Authority
- CN
- China
- Prior art keywords
- sheep placenta
- placenta dish
- dish
- sheep
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000002826 placenta Anatomy 0.000 title claims abstract description 155
- 238000000034 method Methods 0.000 title description 7
- 235000013376 functional food Nutrition 0.000 title description 3
- 239000007788 liquid Substances 0.000 claims abstract description 55
- 235000013305 food Nutrition 0.000 claims abstract description 37
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 35
- 239000000284 extract Substances 0.000 claims abstract description 25
- 230000000694 effects Effects 0.000 claims abstract description 19
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 15
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 239000002775 capsule Substances 0.000 claims abstract description 9
- 108090000526 Papain Proteins 0.000 claims abstract description 8
- 229920001184 polypeptide Polymers 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
- 241001494479 Pecora Species 0.000 claims description 148
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 36
- 238000002360 preparation method Methods 0.000 claims description 28
- 102000004190 Enzymes Human genes 0.000 claims description 23
- 108090000790 Enzymes Proteins 0.000 claims description 23
- 229940088598 enzyme Drugs 0.000 claims description 23
- 235000016709 nutrition Nutrition 0.000 claims description 22
- 230000035764 nutrition Effects 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 20
- 102000019197 Superoxide Dismutase Human genes 0.000 claims description 16
- 108010012715 Superoxide dismutase Proteins 0.000 claims description 16
- 238000005516 engineering process Methods 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 14
- 235000020995 raw meat Nutrition 0.000 claims description 14
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 13
- 229910052804 chromium Inorganic materials 0.000 claims description 13
- 239000011651 chromium Substances 0.000 claims description 13
- 239000007787 solid Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 238000000502 dialysis Methods 0.000 claims description 11
- 238000012856 packing Methods 0.000 claims description 11
- 206010012601 diabetes mellitus Diseases 0.000 claims description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 9
- 238000007710 freezing Methods 0.000 claims description 9
- 230000008014 freezing Effects 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 8
- 239000002504 physiological saline solution Substances 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 8
- 230000007062 hydrolysis Effects 0.000 claims description 7
- 238000006460 hydrolysis reaction Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000011149 active material Substances 0.000 claims description 6
- 238000000108 ultra-filtration Methods 0.000 claims description 6
- 235000011194 food seasoning agent Nutrition 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 4
- 229920001503 Glucan Polymers 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 240000004922 Vigna radiata Species 0.000 claims description 4
- 235000010721 Vigna radiata var radiata Nutrition 0.000 claims description 4
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 claims description 4
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 4
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 4
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 4
- 239000001527 calcium lactate Substances 0.000 claims description 4
- 229960002401 calcium lactate Drugs 0.000 claims description 4
- 235000011086 calcium lactate Nutrition 0.000 claims description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 4
- 235000021552 granulated sugar Nutrition 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 4
- 235000012907 honey Nutrition 0.000 claims description 4
- 239000001630 malic acid Substances 0.000 claims description 4
- 235000011090 malic acid Nutrition 0.000 claims description 4
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 4
- 238000006396 nitration reaction Methods 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 230000003068 static effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- 235000010447 xylitol Nutrition 0.000 claims description 4
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 4
- 229960002675 xylitol Drugs 0.000 claims description 4
- 239000000811 xylitol Substances 0.000 claims description 4
- 239000011701 zinc Substances 0.000 claims description 4
- 239000011670 zinc gluconate Substances 0.000 claims description 4
- 235000011478 zinc gluconate Nutrition 0.000 claims description 4
- 229960000306 zinc gluconate Drugs 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000001900 immune effect Effects 0.000 claims description 3
- 101000868474 Mus musculus Sialoadhesin Proteins 0.000 claims description 2
- 210000004698 lymphocyte Anatomy 0.000 claims description 2
- 230000003301 hydrolyzing effect Effects 0.000 abstract 2
- 239000004365 Protease Substances 0.000 abstract 1
- 230000000975 bioactive effect Effects 0.000 abstract 1
- 230000036039 immunity Effects 0.000 abstract 1
- 229940055729 papain Drugs 0.000 abstract 1
- 235000019834 papain Nutrition 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 13
- 238000001556 precipitation Methods 0.000 description 10
- 239000000047 product Substances 0.000 description 5
- 239000011575 calcium Substances 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 241000283707 Capra Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000002605 anti-dotal effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 239000007963 capsule composition Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002281 placental hormone Substances 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
A functional ewe placenta food 'oral polypeptide liquid' or 'polypeptide capsule' is prepared from the fresh ewe placenta through extracting active component to obtain liquid extract A, hydrolyzing the residual deposit and stored ewe placenta by papain and trypsase and fermenting to obtain hydrolytic liquid B, proportional mixing, dispensing, filtering and sterilizing. Its advantages are rich in bioactive substance and active polypeptide, and high effect to regulate immunity and delay sanility.
Description
Technical field the present invention relates to a kind of food, particularly the goat series food.
Background technology mainly concentrates on fields such as bio-pharmaceuticals and cosmetics for the Application and Development of sheep placenta dish at present, utilizes active material in the sheep placenta dish to combine with other Chinese herbal medicine or chemical reagent and develops various sheep placenta dish biologic products, health products or cosmetic skin care liquid etc.And the Application and Development in food industry is less relatively, and the product of having developed has full powder of sheep placenta dish and activation sheep placental hormone etc., and the technology of employing mainly contains tissue homogenate, vacuum drying and lixiviate etc.Though the sheep placenta dish food of more than developing has kept its biologically active effectively, molecular weight is all bigger, and active peptide and amino acid content are very little, therefore is unfavorable for absorption by human body.
Summary of the invention the present invention is by the extracting of sheep placenta dish natural active matter and two aspect developing sheep placentas of proteolysis dish functional food.Be that raw material adopts ultrafiltration or tissue grinding-homogenate-dialysis to carry out the extraction of active material at first with fresh sheep placenta dish, precipitation part after then active material being extracted and sheep placenta dish are stocked raw material and are carried out enzymolysis, merging extract and enzymolysis liquid and promptly get sheep placenta dish food stoste, is that base-material is made the functionality series health food that has sheep placenta dish biologically active and be rich in active peptide with this stoste again.
One, the preparation method of sheep placenta dish food stoste
1, the preparation of sheep placenta dish extract, it can be realized by dual mode
1. ultrafiltration
Get fresh sheep placenta dish, clean and remove the manadesma part, shred the back and add 1～2 times physiological saline (W/V), carry out tissue homogenate, then with sheep placenta dish homogenate in refrigerated centrifuge centrifugal 20～30 minutes in 7000～8000r/min.Get supernatant and carry out ultrafiltration, inlet pressure is 20～30Psi, and outlet pressure is 2～4Psi.Collecting not, filtration fraction is sheep placenta dish extract (A liquid); Centrifugation partly is used to prepare enzymolysis liquid.
2. organize grinding-homogenate-dialysis
Get fresh sheep placenta dish, clean and remove manadesma, weigh after shredding, organize grinding, add the physiological saline of 1～2 times of sheep placenta dish weight, carry out the high speed tissue homogenate, the placenta after the homogenate is uniform meat gruel shape, then the homogenate of sheep placenta dish was dissolved under 25 ℃ after under-30 ℃～-40 ℃ freezing 48 hours, freeze so repeatedly molten 2～3 times.Again through 5000～70000r/min refrigerated centrifuge 25～30 minutes, got the static dialysis of supernatant 24～48 hours or the vacuum dialysis can obtain the sheep placenta property vitalized extract (A liquid) in 10～12 hours; Centrifugation partly is used to prepare enzymolysis liquid.
2, take off the preparation of raw meat enzymolysis liquid
Clean after the freezing sheep placenta dish of stocking dissolved, remove manadesma, processing such as shred, then with itself and 1 in precipitation weigh after partially mixed, 10～15 times of water that add sheep placenta dish weight carry out tissue homogenate, the homogenate heating was boiled 10～15 minutes, be cooled to 50～55 ℃, regulate pH value to 7.0～7.5, carry out two enzyme combined hydrolysis by 1: 1 enzyme papain and the trypsase of adding than simultaneously alive, total enzyme concentration is 3000～3200u/g material, enzymolysis time 3 hours.Enzymolysis finishes the back to be handled in 90 ℃ of heating 10min enzyme that goes out, and filtering, be concentrated into content of peptides then is 0.10g/ml, sheep placenta dish hydrolyzate.Adopt yeast fermentation method that sheep placenta dish hydrolyzate is taken off raw meat and handle, method is to add 0.8～1.2% dusty yeast to cultivate 50～60 minutes down in 30～35 ℃ in hydrolyzate.
3, the preparation of sheep placenta dish food stoste
With the sheep placenta dish extract of preparation in 1,2 with take off the raw meat hydrolyzate by proper proportion mixed sheep placenta dish food stoste.
Two, adopt above-mentioned sheep placenta dish food stoste can prepare the following functional health-care food that has immunocompetence and be rich in low molecular peptide.
1, sheep placenta dish nutrition oral administration
1. be allocated as the consumption demand that adapts to different crowd, adopt the dissimilar sheep placenta dish nutrition oral administration of multiple formulations modulation.
A, child nutrition enhanced type are pressed sheep placenta dish food stoste weight and are added 6～8% sucrose, 0.023～0.046g/kg calcium lactate, 195～545mg/kg zinc gluconate, 0.010～0.015% essence, the back that stirs is nitration mixture adjust pH to 3～4 of 1: 1 malic acid and citric acid with weight ratio.
B, diabetes patient's tailored version Organic Chromium have the good effect of preventing and treating diabetes.Add rich chromium (chromium content is 50 μ g/ml) mung bean sprouts juice 4～6% by sheep placenta dish food stoste weight, xylitol 7～9%, glucan 0.2～0.4%, back citric acid adjust pH to 3～4 stir.Suitable diabetic takes.
C, plain edition are pressed sheep placenta dish food stoste weight and are added 2% granulated sugar, 0.04～0.06% honey element, and 0.010～0.015% essence, back citric acid adjust pH to 3～4 stir.
2. filter the sheep placenta dish nutrient solution after the above-mentioned seasoning is filtered through 400 eye mesh screens.
3. canned, sterilization are injected into above-mentioned sheep placenta dish nutrient solution in the brown oral liquid bottle with can packing machine, adopt 63 ℃, 30 minutes pasteurize technology.
As follows by the sheep placenta dish nutrition oral administration composition that said method obtains:
A, child nutrition enhanced type
Solid content 10～20%
Protein content 25%～30%
Peptide yield 50～60%
Superoxide dismutase activity 2000～2500u/ml
Ca 2～4mg/ml
Zn 18～50mg/ml
B, diabetes patient's tailored version
Solid content 10～20%
Protein content 25～30%
Peptide yield 50～60%
Superoxide dismutase activity 2000～2500u/ml
Chromium 2～3 μ g/ml
C, plain edition
Solid content 5～15%
Protein content 25～30%
Peptide yield 50～60%
Superoxide dismutase activity 2000～2500u/ml
2, sheep placenta dish biologically active capsule
Adopt vacuum freeze drying, above-mentioned sheep placenta dish food stoste is placed freeze drier, carry out drying, make moisture≤4% in-40 ℃ of following vacuum 133 * 10-3Pa, obtain sheep placenta dish polypeptide powder, more encapsulated getting product under 50～60% relative humidity.
As follows by the sheep placenta dish biologically active capsule composition that said method makes:
Superoxide dismutase activity 4000～6000u/g
Protein content 50～60%
Solubility in acid peptide yield 60～70%
Moisture 4%
E-garland experimental result: sheep placenta dish biologically active capsule has the immunologic competence that promotes that sheep erythrocyte receptor recovers on the human lymphocyte.
The present invention has following advantage:
1, adopt membrane technology and biological enzymolysis technology to carry out the extraction of sheep placenta dish bioactivator and the preparation of hydrolyzate respectively, then sheep placenta dish extract and hydrolyzate are united two into one, make the sheep placenta dish polypeptide nutrient fluid of biologically active, and then prepare sheep placenta dish nutrition oral administration, sheep placenta dish biologically active capsule, to satisfy people's multiple demand.
2, the present invention extracts and proteolysis two parts in conjunction with active material, both overcome the shortcoming that natural activity reduces in the hydrolyzate, though remedied also that extract is rich in natural active matter but the very little deficiency of active peptide and amino acid content, accomplished making full use of to the sheep placenta dish, products obtained therefrom not only possesses immunological regulation and antidotal function, is more conducive to digest and assimilate.
3, the present invention adopts yeast fermentation method to take off raw meat, and method is simply effective, and operative temperature does not almost have influence below 35 ℃ to the active component in the sheep placenta dish.
The specific embodiment
Example 1
The preparation of sheep placenta dish extract (A liquid): get fresh sheep placenta dish, clean, remove blood vessel, fat and connective tissue part, shred, the physiological saline (W/V) that adds 1 times carries out tissue homogenate, then with sheep placenta dish homogenate in refrigerated centrifuge in 7000r/min centrifugal 30 minutes.Get supernatant and carry out ultrafiltration, inlet pressure is 20Psi, and outlet pressure is 2Psi.Collecting not, filtration fraction is sheep placenta dish extract (A liquid); The precipitation part is used to prepare enzymolysis liquid.
Take off the preparation of raw meat sheep placenta dish hydrolyzate (B liquid): processing such as clean, remove manadesma after the freezing sheep placenta dish of stocking is dissolved, shred, then the precipitation in the preparation of itself and extract is weighed after partially mixed, 10 times of water that add sheep placenta dish weight carry out tissue homogenate, the homogenate heating was boiled 10 minutes, be cooled to 52 ℃, regulate pH value to 7.0, carry out two enzyme combined hydrolysis by 1: 1 enzyme papain and the trypsase of adding than simultaneously alive, total enzyme concentration is the 3000u/g material, enzymolysis time 3 hours.Enzymolysis finishes the back to be handled in 90 ℃ of heating 10min enzyme that goes out, filtering, be concentrated into content of peptides then is that 0.10g/ml gets sheep placenta dish hydrolyzate, the dusty yeast of adding 1.2% was cultivated 50 minutes down in 30 ℃ in hydrolyzate, made the sheep placenta dish hydrolyzate (B liquid) of removing fishy smell.
With the above-mentioned A liquid that makes and B liquid in 1: 10 ratio mix sheep placenta dish food stoste.
Example 2
The preparation of sheep placenta dish extract (A liquid): get fresh sheep placenta dish, clean, remove blood vessel, fat and connective tissue part, shred, the physiological saline (W/V) that adds 2 times carries out tissue homogenate, then with sheep placenta dish homogenate in refrigerated centrifuge in 8000r/min centrifugal 20 minutes.Get supernatant and carrying out ultrafiltration, inlet pressure is 30Psi, and outlet pressure is 4Psi.Collecting not, filtration fraction is sheep placenta dish extract (A liquid); The precipitation part is used to prepare enzymolysis liquid.
Take off the preparation of raw meat sheep placenta dish hydrolyzate (B liquid): processing such as clean, remove manadesma after the freezing sheep placenta dish of stocking is dissolved, shred, then the precipitation in the preparation of itself and extract is weighed after partially mixed, 15 times of water that add sheep placenta dish weight carry out tissue homogenate, the homogenate heating was boiled 10 minutes, be cooled to 55 ℃, regulate pH value to 7.5, carry out two enzyme combined hydrolysis by 1: 1 enzyme papain and the trypsase of adding than simultaneously alive, total enzyme concentration is the 3200u/g material, enzymolysis time 3 hours.Enzymolysis finishes the back to be handled in 90 ℃ of heating 10min enzyme that goes out, and filtering, be concentrated into content of peptides then is that 0.10g/ml gets sheep placenta dish hydrolyzate (B liquid).The dusty yeast of adding 0.8% was cultivated 55 minutes down in 35 ℃ in hydrolyzate, made the sheep placenta dish hydrolyzate (B liquid) of removing fishy smell.
With the above-mentioned A liquid that makes and B liquid in 1: 5 ratio mix sheep placenta dish food stoste.
Example 3
The preparation of sheep placenta dish extract (A liquid): get fresh sheep placenta dish, clean and remove the manadesma part, weigh after shredding, organize grinding, add the physiological saline of 1 times of sheep placenta dish weight again, carry out the high speed tissue homogenate, the placenta after the homogenate is uniform meat gruel shape, then the homogenate of sheep placenta dish was dissolved under 25 ℃ after under-30 ℃ freezing 48 hours, freeze so repeatedly molten 3 times.Again through 5000r/min refrigerated centrifuge 30 minutes, got the static dialysis of supernatant 24 hours or the vacuum dialysis can obtain the sheep placenta property vitalized extract (A liquid) in 12 hours.The precipitation part is used to prepare enzymolysis liquid.
Take off the preparation of raw meat sheep placenta dish hydrolyzate (B liquid): processing such as clean, remove manadesma after the freezing sheep placenta dish of stocking is dissolved, shred, then the precipitation in the preparation of itself and extract is weighed after partially mixed, 15 times of water that add sheep placenta dish weight carry out tissue homogenate, the homogenate heating was boiled 15 minutes, be cooled to 50 ℃, regulate pH value to 7.0, carry out two enzyme combined hydrolysis by 1: 1 enzyme papain and the trypsase of adding than simultaneously alive, total enzyme concentration is the 3000u/g material, enzymolysis time 3 hours.Enzymolysis finishes the back to be handled in 90 ℃ of heating 10min enzyme that goes out, filtering, be concentrated into content of peptides then is 0.10g/ml, get sheep placenta dish hydrolyzate, the dusty yeast of adding 0.8% was cultivated 55 minutes down in 35 ℃ in hydrolyzate, made the sheep placenta dish hydrolyzate (B liquid) of removing fishy smell.
With the above-mentioned A liquid that makes and B liquid in 1: 10 ratio mix sheep placenta dish food stoste.
Example 4
The preparation of sheep placenta dish extract (A liquid): get fresh sheep placenta dish, clean and remove the manadesma part, weigh after shredding, organize grinding, add the physiological saline of 2 times of sheep placenta dish weight again, carry out the high speed tissue homogenate, the placenta after the homogenate is uniform meat gruel shape, then the homogenate of sheep placenta dish was dissolved under 25 ℃ after under-40 ℃ freezing 48 hours, freeze so repeatedly molten 2 times.Again through 70000r/min refrigerated centrifuge 25 minutes, got the static dialysis of supernatant 48 hours or the vacuum dialysis can obtain the sheep placenta property vitalized extract (A liquid) in 10 hours.The precipitation part is used to prepare enzymolysis liquid.
Take off the preparation of raw meat sheep placenta dish hydrolyzate (B liquid): processing such as clean, remove manadesma after the freezing sheep placenta dish of stocking is dissolved, shred, then with itself and 1 in precipitation weigh after partially mixed, 10 times of water that add sheep placenta dish weight carry out tissue homogenate, the homogenate heating was boiled 15 minutes, be cooled to 55 ℃, regulate pH value to 7.5, carry out two enzyme combined hydrolysis by 1: 1 enzyme papain and the trypsase of adding than simultaneously alive, total enzyme concentration is the 3200u/g material, enzymolysis time 3 hours.Enzymolysis finishes the back to be handled in 90 ℃ of heating 10min enzyme that goes out, and filtering, be concentrated into content of peptides then is 0.10g/ml, sheep placenta dish hydrolyzate.The dusty yeast of adding 1.2% was cultivated 60 minutes down in 30 ℃ in hydrolyzate, made the sheep placenta dish hydrolyzate (B liquid) of removing fishy smell.
With the above-mentioned A liquid that makes and B liquid in 1: 5 ratio mix sheep placenta dish food stoste.
Example 5
Get the sheep placenta dish food stoste that obtains by example 1, add 6～8% sucrose by weight, 0.023g/kg calcium lactate, 195mg/kg zinc gluconate, 0.010% essence, stirring back is 1: 1 the malic acid and the nitration mixture adjust pH to 3 of citric acid with weight ratio, after 400 eye mesh screens filter, use can packing machine to be injected in the brown oral liquid bottle, adopt 63 ℃ at last, 30 minutes pasteurize technology promptly makes " child nutrition enhanced type " sheep placenta dish nutrition oral administration.Its composition is as follows:
Solid content 10%
Protein content 25%
Peptide yield 55%
Superoxide dismutase activity 2000u/ml
Ca 2mg/ml
Zn 18mg/ml
Example 6
Get the sheep placenta dish food stoste that obtains by example 2, add 8% sucrose by weight, 0.046g/kg calcium lactate, 545mg/kg zinc gluconate, 0.015% essence, stirring back is 1: 1 the malic acid and the nitration mixture adjust pH to 3 of citric acid with weight ratio, after 400 eye mesh screens filter, use can packing machine to be injected in the brown oral liquid bottle, adopt 63 ℃ at last, 30 minutes pasteurize technology promptly makes " child nutrition enhanced type " sheep placenta dish nutrition oral administration.Its composition is as follows:
Solid content 20%
Protein content 30%
Peptide yield 55%
Superoxide dismutase activity 2500u/ml
Ca 4mg/ml
Zn 48mg/ml
Example 7
Get the sheep placenta dish food stoste that obtains by example 3, add rich chromium (chromium content is 50 μ g/ml) mung bean sprouts juice 4% by weight, xylitol 7%, glucan 0.2%, back citric acid adjust pH to 3 stirs.After 400 eye mesh screens filter, use can packing machine to be injected in the brown oral liquid bottle, adopt 63 ℃ at last, 30 minutes pasteurize technology promptly makes " diabetes patient's tailored version " sheep placenta dish nutrition oral administration.Suitable diabetic takes.Its composition is as follows:
Solid content 15%
Protein content 28%
Peptide yield 55%
Superoxide dismutase activity 2000u/ml
Chromium 2 μ g/ml
Example 8
Get the sheep placenta dish food stoste that obtains by example 4, add rich chromium (chromium content is 50 μ g/ml) mung bean sprouts juice 6% by weight, xylitol 9%, glucan 0.4%, back citric acid adjust pH to 4 stirs.After 400 eye mesh screens filter, use can packing machine to be injected in the brown oral liquid bottle, adopt 63 ℃ at last, 30 minutes pasteurize technology promptly makes " diabetes patient's tailored version " sheep placenta dish nutrition oral administration.Suitable diabetic takes.Its composition is as follows:
Solid content 20%
Protein content 30%
Peptide yield 55%
Superoxide dismutase activity 2500u/ml
Chromium 3 μ g/ml
Example 9
Get the sheep placenta dish food stoste that obtains by example 1, add 2% granulated sugar by weight, 0.04% honey element, 0.010% essence, back citric acid adjust pH to 3 stirs.After 400 eye mesh screens filter, use can packing machine to be injected in the brown oral liquid bottle, adopt 63 ℃ at last, 30 minutes pasteurize technology promptly makes " plain edition " sheep placenta dish nutrition oral administration.Its composition is as follows:
Solid content 5%
Protein content 25%
Peptide yield 50%
Superoxide dismutase activity 2000u/ml
Example 10
Get the sheep placenta dish food stoste that obtains by example 2, add 2% granulated sugar by weight, 0.06% honey element, 0.015% essence, back citric acid adjust pH to 4 stirs.After 400 eye mesh screens filter, use can packing machine to be injected in the brown oral liquid bottle, adopt 63 ℃ at last, 30 minutes pasteurize technology promptly makes " plain edition " sheep placenta dish nutrition oral administration.Its composition is as follows:
Solid content 15%
Protein content 30%
Peptide yield 60%
Superoxide dismutase activity 2000u/ml
Example 11
Get the sheep placenta dish food stoste that is obtained by example 3, put into freeze drier, the control temperature makes moisture≤4% at-40 ℃, vacuum 133 * 10-3Pa.During the sheep placenta dish polypeptide powder that freeze drying is made incapsulates, be sheep placenta dish biologically active capsule, its composition is as follows:
Superoxide dismutase activity 5000u/g
Total protein 50%
Solubility in acid peptide yield 70%
Moisture≤4%
Example 12
Get the sheep placenta dish food stoste that is obtained by example 4, put into freeze drier, the control temperature makes moisture≤4% at-40 ℃, vacuum 133 * 10-3Pa.During the sheep placenta dish polypeptide powder that freeze drying is made incapsulates, promptly get sheep placenta dish biologically active capsule, its composition is as follows:
Superoxide dismutase activity 6000u/g
Total protein 60%
Solubility in acid peptide yield 70%
Moisture≤4%
Annotate: the sheep placenta dish of selecting for use meets the provisions of the relevant regulations issued by the State through sanitary inspection.
Claims (10)
1, a kind of preparation method of sheep placenta dish food stoste is characterized in that:
1. active material extracts
A, add the physiological saline (W/V) of 1～2 times of fresh sheep placenta dish weight, carry out tissue homogenate,
B, with above-mentioned sheep placenta dish homogenate in refrigerated centrifuge centrifugal 20～30 minutes in 7000～8000r/min,
C, get supernatant and carry out ultrafiltration, inlet pressure is 20～30Psi, and outlet pressure is 2～4Psi,
2. take off the preparation of raw meat enzymolysis liquid
A, raw material are handled: 10～15 times of water that add sheep placenta dish weight carry out tissue homogenate,
B, enzymolysis: above-mentioned homogenate heating was boiled 10～15 minutes, adjust the temperature to 50～55 ℃, pH value to 7.0～7.5, carry out two enzyme combined hydrolysis by 1: 1 enzyme papain and the trypsase of adding than simultaneously alive, total enzyme concentration is 3000～3200u/g material, enzymolysis time 3 hours
C, enzyme goes out: with above-mentioned enzymolysis liquid in 90 ℃ of heating 10min,
D, filtration, concentrate: enzymolysis liquid goes out and filters behind the enzyme, and being concentrated into content of peptides is 0.10g/ml, sheep placenta dish hydrolyzate,
E, hydrolyzate take off raw meat to be handled: in sheep placenta dish hydrolyzate, add 0.8～1.2% dusty yeast and cultivated 50～60 minutes down in 30～35 ℃,
3. the preparation of sheep placenta dish food stoste
In 1: 5～1: 10 ratio mix 1., 2. in the gained extract with take off the raw meat hydrolyzate and promptly get sheep placenta dish food stoste.
2, a kind of preparation method of sheep placenta dish food stoste is characterized in that:
1. active material extracts
A, tissue grind: the sheep placenta dish after the using-system mill will shred is organized grinding,
B, homogenate: after placenta grinds, add the physiological saline (W/V) of 1～2 times of its weight, carry out tissue homogenate,
C, freeze molten: the homogenate of sheep placenta dish is dissolved under 25 ℃ after freezing 48 hours down at-30 ℃～40 ℃, freezes so repeatedly 2～3 times,
D, centrifugal: freeze homogenate after molten through 5000～70000r/min refrigerated centrifuge 25～30 minutes,
E, dialysis: got the static dialysis of supernatant 24～48 hours or vacuum dialysis can obtain the sheep placenta property vitalized extract in 10～12 hours,
2. take off the preparation of raw meat enzymolysis liquid
A, raw material are handled: 10～15 times of water that add sheep placenta dish weight carry out tissue homogenate,
B, enzymolysis: above-mentioned homogenate heating was boiled 10～15 minutes, adjust the temperature to 50～55 ℃, pH value to 7.0～7.5, carry out two enzyme combined hydrolysis by 1: 1 enzyme papain and the trypsase of adding than simultaneously alive, total enzyme concentration is 3000～3200u/g material, enzymolysis time 3 hours
C, enzyme goes out: with above-mentioned enzymolysis liquid in 90 ℃ of heating 10min,
D, filtration, concentrate: enzymolysis liquid goes out and filters behind the enzyme, and being concentrated into content of peptides is 0.10g/ml, sheep placenta dish hydrolyzate,
E, hydrolyzate take off raw meat to be handled: in sheep placenta dish hydrolyzate, add 0.8～1.2% dusty yeast and cultivated 50～60 minutes down in 30～35 ℃,
3. the preparation of sheep placenta dish food stoste
In 1: 5～1: 10 ratio mix 1., 2. in the gained extract with take off the raw meat hydrolyzate and promptly get sheep placenta dish food stoste.
3, a kind of preparation method of child nutrition enhanced type sheep placenta dish nutrition oral administration is characterized in that:
A, allotment: press sheep placenta dish food stoste weight and add 6～8% sucrose, 0.023～0.046g/kg calcium lactate, 195～545mg/kg zinc gluconate, 0.010～0.015% essence, the back that stirs is nitration mixture adjust pH to 3～4 of 1: 1 malic acid and citric acid with weight ratio
B, filtration: the sheep placenta dish nutrient solution after the above-mentioned seasoning is filtered through 400 eye mesh screens,
C, canned, sterilization: above-mentioned sheep placenta dish nutrient solution is injected brown oral liquid bottle by can packing machine, adopt 63 ℃, 30 minutes pasteurize technology is sterilized.
4, child nutrition enhanced type sheep placenta dish nutrition oral administration according to claim 3 is characterized in that:
Solid content 10～20%
Protein content 25%～30%
Peptide yield 50～60%
Superoxide dismutase activity 2000～2500u/ml
Ca 2～4mg/ml
Zn 18～50mg/ml
5, a kind of preparation method of diabetes patient's tailored version sheep placenta dish nutrition oral administration is characterized in that:
A, allotment: add rich chromium (chromium content is 50 μ g/ml) mung bean sprouts juice 4～6% by sheep placenta dish food stoste weight, xylitol 7～9%, glucan 0.2～0.4%, back citric acid adjust pH to 3～4 that stir,
B, filtration: the sheep placenta dish nutrient solution after the above-mentioned seasoning is filtered through 400 eye mesh screens,
C, canned, sterilization: above-mentioned sheep placenta dish nutrient solution is injected brown oral liquid bottle by can packing machine, adopt 63 ℃, 30 minutes pasteurize technology is sterilized.
6, diabetes patient's tailored version sheep placenta dish nutrition oral administration according to claim 5 is characterized in that:
Solid content 10～20%
Protein content 25～30%
Peptide yield 50～60%
Superoxide dismutase activity 2000～2500u/ml
Chromium 2～3 μ g/ml
7, a kind of preparation method of plain edition sheep placenta dish nutrition oral administration is characterized in that:
A, seasoning: press sheep placenta dish food stoste weight and add 2% granulated sugar, 0.04～0.06% honey element, 0.010～0.015% essence, back citric acid adjust pH to 3～4 that stir,
B, filtration: the sheep placenta dish nutrient solution after the above-mentioned seasoning is filtered through 400 eye mesh screens,
C, canned, sterilization: above-mentioned sheep placenta dish nutrient solution is injected brown oral liquid bottle by can packing machine, adopt 63 ℃, 30 minutes pasteurize technology.
8, plain edition sheep placenta dish nutrition oral administration according to claim 7 is characterized in that:
Solid content 5～15%
Protein content 25～30%
Peptide yield 50～60%
Superoxide dismutase activity 2000～2500u/ml
9, a kind of preparation method of sheep placenta dish biologically active capsule is characterized in that:
A, drying: sheep placenta dish food stoste is placed freeze drier, carry out drying, make moisture≤4% in-40 ℃ of following vacuum 133 * 10-3Pa,
B, packing: it is encapsulated then that the relative humidity environment of sheep placenta dish polypeptide powder in 50～60% is adjusted downward to room temperature.
10, sheep placenta dish biologically active capsule according to claim 9 is characterized in that:
Superoxide dismutase activity 4000～6000u/g
Protein content 50～60%
Solubility in acid peptide yield 60～70%
Moisture 4%
E-garland experimental result: sheep placenta dish biologically active capsule has the immunologic competence that promotes that sheep erythrocyte receptor recovers on the human lymphocyte.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101048546A CN1277489C (en) | 2003-10-14 | 2003-10-14 | Functional food contg. ewe placenta, and its prepn. method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101048546A CN1277489C (en) | 2003-10-14 | 2003-10-14 | Functional food contg. ewe placenta, and its prepn. method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1537465A true CN1537465A (en) | 2004-10-20 |
| CN1277489C CN1277489C (en) | 2006-10-04 |
Family
ID=34333539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101048546A Expired - Lifetime CN1277489C (en) | 2003-10-14 | 2003-10-14 | Functional food contg. ewe placenta, and its prepn. method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1277489C (en) |
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100434530C (en) * | 2005-04-22 | 2008-11-19 | 黄磊 | Proteinic small peptide of Placenta and preparation method thereof |
| CN102329842A (en) * | 2011-07-12 | 2012-01-25 | 湖州康海斯生物科技有限公司 | Lamb placenta polypeptide and preparation method thereof |
| CN102329841A (en) * | 2011-07-12 | 2012-01-25 | 湖州康海斯生物科技有限公司 | Lamb placenta protein and preparation method thereof |
| CN102485030A (en) * | 2010-12-05 | 2012-06-06 | 杜冰 | Method for preparing sheep placenta health care chafing dish base flavouring by using enzymatic hydrolysis |
| CN103330048A (en) * | 2013-07-19 | 2013-10-02 | 内蒙古迦妮生物科技开发有限公司 | Preparation methods of sheep placenta polypeptide powder and soluble granules |
| CN103961404A (en) * | 2014-05-23 | 2014-08-06 | 汤臣倍健股份有限公司 | Health-care composition and health-care product |
| CN105030630A (en) * | 2014-12-12 | 2015-11-11 | 黄宗堂 | Stem cell eye cream and preparation method thereof |
| CN105030631A (en) * | 2014-12-12 | 2015-11-11 | 黄宗堂 | Stem cell face cream and preparation method thereof |
| CN105147575A (en) * | 2014-12-12 | 2015-12-16 | 黄宗堂 | Hair restorer containing stem cells and preparation method thereof |
| CN106692943A (en) * | 2017-02-09 | 2017-05-24 | 赵德润 | Method for preparing aspongopus bioactive peptide |
| CN106889295A (en) * | 2017-02-09 | 2017-06-27 | 赵德润 | A kind of preparation method of live body sea stichopus japonicus active peptide |
| CN107254502A (en) * | 2017-07-26 | 2017-10-17 | 兰州名德农牧科技有限公司 | A kind of method that animal protease prepares Goat Placenta-peptide |
| CN107412136A (en) * | 2017-09-04 | 2017-12-01 | 银川凤仪堂生物工程有限公司 | Goat Placenta enzymatic hydrolysis and fermentation concentrate and preparation technology for cosmetic material |
| CN107519206A (en) * | 2017-10-20 | 2017-12-29 | 陕西干细胞工程有限公司 | A kind of Human plactnta freeze-dried powder preparation and preparation method thereof |
| CN108142939A (en) * | 2018-02-05 | 2018-06-12 | 内蒙古康肽生物工程有限公司 | A kind of oral liquid of the factor containing placenta stem-cell and preparation method thereof |
| CN109222110A (en) * | 2018-07-17 | 2019-01-18 | 闵红岗 | A kind of antifatigue soluble granule and preparation method of the polypeptide dry powder containing Goat Placenta |
| CN109568345A (en) * | 2019-01-04 | 2019-04-05 | 湖南海济药业有限公司 | A kind of strengthen immunity, oral solution of anti-aging and preparation method thereof |
| CN109943614A (en) * | 2019-04-02 | 2019-06-28 | 河北康进生物科技有限公司 | A kind of preparation method and applications of Goat Placenta active peptide |
| CN110478305A (en) * | 2019-08-21 | 2019-11-22 | 银川凤仪堂生物工程有限公司 | A kind of preparation process and its application of sheep placenta extract |
| CN110613128A (en) * | 2019-07-24 | 2019-12-27 | 宁波华子制药设备制造有限公司 | Low-temperature extraction production process of active sheep placenta |
| CN111820404A (en) * | 2020-07-07 | 2020-10-27 | 肖启森 | Sheep embryo extract beautifying oral liquid and preparation method thereof |
| CN114592024A (en) * | 2022-04-01 | 2022-06-07 | 珠海市华喜生物科技有限公司 | Sheep placenta polypeptide and preparation method and application thereof |
| CN114766619A (en) * | 2022-05-30 | 2022-07-22 | 中山市美太保健制品有限公司 | Health-care product oral liquid for delaying senility and preparation method thereof |
-
2003
- 2003-10-14 CN CNB2003101048546A patent/CN1277489C/en not_active Expired - Lifetime
Cited By (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100434530C (en) * | 2005-04-22 | 2008-11-19 | 黄磊 | Proteinic small peptide of Placenta and preparation method thereof |
| CN102485030A (en) * | 2010-12-05 | 2012-06-06 | 杜冰 | Method for preparing sheep placenta health care chafing dish base flavouring by using enzymatic hydrolysis |
| CN102329842A (en) * | 2011-07-12 | 2012-01-25 | 湖州康海斯生物科技有限公司 | Lamb placenta polypeptide and preparation method thereof |
| CN102329841A (en) * | 2011-07-12 | 2012-01-25 | 湖州康海斯生物科技有限公司 | Lamb placenta protein and preparation method thereof |
| CN102329842B (en) * | 2011-07-12 | 2014-10-29 | 湖州康海斯生物科技有限公司 | Lamb placenta polypeptide and preparation method thereof |
| CN103330048A (en) * | 2013-07-19 | 2013-10-02 | 内蒙古迦妮生物科技开发有限公司 | Preparation methods of sheep placenta polypeptide powder and soluble granules |
| CN103961404A (en) * | 2014-05-23 | 2014-08-06 | 汤臣倍健股份有限公司 | Health-care composition and health-care product |
| CN105030630A (en) * | 2014-12-12 | 2015-11-11 | 黄宗堂 | Stem cell eye cream and preparation method thereof |
| CN105030631A (en) * | 2014-12-12 | 2015-11-11 | 黄宗堂 | Stem cell face cream and preparation method thereof |
| CN105147575A (en) * | 2014-12-12 | 2015-12-16 | 黄宗堂 | Hair restorer containing stem cells and preparation method thereof |
| CN106692943A (en) * | 2017-02-09 | 2017-05-24 | 赵德润 | Method for preparing aspongopus bioactive peptide |
| CN106889295A (en) * | 2017-02-09 | 2017-06-27 | 赵德润 | A kind of preparation method of live body sea stichopus japonicus active peptide |
| CN107254502A (en) * | 2017-07-26 | 2017-10-17 | 兰州名德农牧科技有限公司 | A kind of method that animal protease prepares Goat Placenta-peptide |
| CN107412136A (en) * | 2017-09-04 | 2017-12-01 | 银川凤仪堂生物工程有限公司 | Goat Placenta enzymatic hydrolysis and fermentation concentrate and preparation technology for cosmetic material |
| CN107519206A (en) * | 2017-10-20 | 2017-12-29 | 陕西干细胞工程有限公司 | A kind of Human plactnta freeze-dried powder preparation and preparation method thereof |
| CN108142939A (en) * | 2018-02-05 | 2018-06-12 | 内蒙古康肽生物工程有限公司 | A kind of oral liquid of the factor containing placenta stem-cell and preparation method thereof |
| CN109222110A (en) * | 2018-07-17 | 2019-01-18 | 闵红岗 | A kind of antifatigue soluble granule and preparation method of the polypeptide dry powder containing Goat Placenta |
| CN109568345A (en) * | 2019-01-04 | 2019-04-05 | 湖南海济药业有限公司 | A kind of strengthen immunity, oral solution of anti-aging and preparation method thereof |
| CN109943614A (en) * | 2019-04-02 | 2019-06-28 | 河北康进生物科技有限公司 | A kind of preparation method and applications of Goat Placenta active peptide |
| CN110613128A (en) * | 2019-07-24 | 2019-12-27 | 宁波华子制药设备制造有限公司 | Low-temperature extraction production process of active sheep placenta |
| CN110478305A (en) * | 2019-08-21 | 2019-11-22 | 银川凤仪堂生物工程有限公司 | A kind of preparation process and its application of sheep placenta extract |
| CN111820404A (en) * | 2020-07-07 | 2020-10-27 | 肖启森 | Sheep embryo extract beautifying oral liquid and preparation method thereof |
| CN114592024A (en) * | 2022-04-01 | 2022-06-07 | 珠海市华喜生物科技有限公司 | Sheep placenta polypeptide and preparation method and application thereof |
| CN114766619A (en) * | 2022-05-30 | 2022-07-22 | 中山市美太保健制品有限公司 | Health-care product oral liquid for delaying senility and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1277489C (en) | 2006-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1277489C (en) | Functional food contg. ewe placenta, and its prepn. method | |
| CN1223283C (en) | Phycomycetes food for improving physical ability of athletes and its preparation method | |
| JP2019516412A (en) | Low molecular weight collagen peptide and method for producing food composition using the same | |
| CN108753874A (en) | A kind of preparation method of small active peptides novel spirulina powder | |
| CN102150836A (en) | Ammonia sugar peptide soft capsule | |
| CN102302195A (en) | Fish bean curd and preparation method thereof | |
| CN112438356A (en) | Multi-element compound peptide solid beverage and preparation method thereof | |
| JP4132635B2 (en) | Uninactivated enzyme-enhanced composition | |
| CN1791419A (en) | Remedy | |
| CN102302161A (en) | Preparation method of ophidine oral liquid | |
| CN1178672C (en) | Process for preparing polypeptide-nucleic acid nutritive liquid of sheep placenta | |
| CN109123036A (en) | Soybean-marrow peptide composition and application | |
| CN101965982A (en) | Method for preparing beverage from silk peptide | |
| CN1260248C (en) | Active lactoprotein extracted from yak milk and its extraction method and use | |
| CN1531399A (en) | Bioactive food additive and bioactive feed additive for preventing iodine deficiency and optimizing iodine metabolism, and food and feed containing the additive | |
| CN106854232A (en) | A kind of preparation method and applications of bone marrow proteins in tumor | |
| JP2001231497A (en) | Functional food | |
| CN105476014A (en) | Method for preparing biological zinc from oysters | |
| KR102085436B1 (en) | How to process food raw materials using raw silkworms | |
| CN1698448A (en) | Polypeptide almond milk and preparation method thereof | |
| CN1920049A (en) | Small molecule bioactive peptide, preparation method, composition and application thereof | |
| CN102273633A (en) | Method for preparing oyster shell peptide oral liquid | |
| CN106173853B (en) | Nutritional composition for reducing blood fat and application thereof | |
| CN1240297C (en) | Peptide milk and its preparation technology | |
| CN108795622A (en) | A kind of semi-fermented arrow thorn wine and its production technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20061004 |