CN1522747A - Drug for treating coronary heart disease or coronary disease and cardiac insufficiency, and its preparation method - Google Patents
Drug for treating coronary heart disease or coronary disease and cardiac insufficiency, and its preparation method Download PDFInfo
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Abstract
The present invention relates to a medicine in treating coronary heart disease or coronary heart disease with complication of cardiac functional insufficiency. It is characterized by that it made rhodiola kirilowii and notoginseng as raw material, red peony root and ginkgo leaf also can also be added. Said invention also discloses its preparation method.
Description
Technical field
The present invention relates to a kind of Chinese herbal medicine composition of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency and preparation method thereof.
Background technology
Coronary heart disease is the abbreviation of coronary heart disease, be a kind of because coronary artery stationarity (atheroma sclerosis) or dynamic property (vasospasm) stenosis or occlusion, the coronary circulation obstacle takes place, cause that the myocardial oxygen pin is unbalance and cause myocardial ischemia-anoxemia or downright bad a kind of heart disease between needing, also claim ischemic heart desease.This sick basic lesion be supply myocardial nutrition material blood vessel--coronary artery has taken place atherosis, mainly show as angina pectoris, arrhythmia, heart failure, may die suddenly.In recent decades, the sickness rate of coronary atherosclerotic heart disease increases gradually in China.Coronary heart disease is because its sickness rate height, the mortality rate height, and serious harm human healthy, thereby b referred to as " the first human killers ".
In the prior art, the common drug of treatment coronary heart disease is to take nitroglycerine tablets, nitroglycerin to cerebrovascular dilating effect clearly, flushed face, headache appear after taking medicine immediately, occur " orthostatic hypotension " when standing and mistake easily takes place faint, elderly patients or the patient who takes medicine for the first time, being seated has dizzy headache sensation after taking medicine, take nitroglycerine tablets for a long time, can produce certain side effect.
Summary of the invention
Technical problem to be solved by this invention is at the deficiencies in the prior art, and a kind of new treatment coronary heart disease or the medicine of coronary heart disease merging cardiac insufficiency are provided, and this drug side effect is little, instant effect.
Another technical problem to be solved by this invention is at the deficiencies in the prior art, has proposed manufacturing method for above mentioned medicine.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency, is characterized in that it is to comprise that Radix Rhodiolae and Radix Notoginseng are the medicament that raw material is made.
Technical problem to be solved by this invention can also further realize by following technical scheme.Above-described a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency is characterized in that wherein the weight proportion of each raw material is,
Radix Rhodiolae 100-400 Radix Notoginseng 100-400
Technical problem to be solved by this invention can also further realize by following technical scheme.Above-described a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency is characterized in, also comprises Radix Paeoniae Rubra in its raw material.
Technical problem to be solved by this invention can also further realize by following technical scheme.Above-described a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency is characterized in that wherein the weight proportion of each raw material is,
Radix Rhodiolae 100-400 Radix Notoginseng 100-400 Radix Paeoniae Rubra 100-400
Technical problem to be solved by this invention can also further realize by following technical scheme.Above-described a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency is characterized in, also comprises Folium Ginkgo in its raw material.
Technical problem to be solved by this invention can also further realize by following technical scheme.Above-described a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency is characterized in that wherein the weight proportion of each raw material is,
Radix Rhodiolae 100-400 Radix Notoginseng 100-400
Radix Paeoniae Rubra 100-400 Folium Ginkgo 100-400
Technical problem to be solved by this invention can also further realize by following technical scheme.Above-described a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency is characterized in that described medicament is said any dosage form on the pharmaceutics.Wherein preferred dosage form is an injection.
Technical problem to be solved by this invention can also further realize by following technical scheme.The present invention is a kind of preparation method for the treatment of the medicine of coronary heart disease or coronary heart disease merging cardiac insufficiency, is characterized in that its step is as follows:
(1) gets Radix Rhodiolae, add 40～80% ethanol and extract, filter at 60～70 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract; Perhaps get Radix Rhodiolae, add water 10-12 and doubly measure decoction 1-3 time, each 1-3 hour, filter, merging filtrate, at 70-80 ℃, reclaim under reduced pressure adds ethanol to containing alcohol amount 60-80% to containing 3-5g crude drug/ml under the-0.5--1.0MPa condition, static 20-30 hour, filter, at 70-80 ℃, decompression recycling ethanol is to there not being the alcohol flavor under the-0.5--1.0MPa condition, continue to be concentrated into and contain 4-6g crude drug/ml, add ethanol to containing alcohol amount 70-90%, add 15-25%NaOH and transfer pH to 7-9, static 20-30 hour, filter, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Notoginseng, carry out percolation, make solvent, flood after 30-50 hour with 90-100% ethanol by percolation, speed with per minute 2～4ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use the 50-70% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(3) get above-mentioned Radix Rhodiolae extract and Radix Notoginseng extract and mix, add the dissolving of injection water, leave standstill, filter, the filtrate ultrafiltration, the embedding ampoule promptly gets injection; Perhaps add 0.5-2.0% freeze-dried powder proppant, make its dissolving, aseptic subpackaged in the sterilization cillin bottle, lyophilization, tamponade, gland promptly gets lyophilized injectable powder; Perhaps add conventional medical accessory, make other any dosage form according to a conventional method, in the preparation of described any dosage form, the content sum of effective ingredient Radix Notoginseng total arasaponins and rhodioside is greater than 25%.
Technical problem to be solved by this invention can also further realize by following technical scheme.The present invention is a kind of preparation method for the treatment of the medicine of coronary heart disease or coronary heart disease merging cardiac insufficiency, is characterized in that its step is as follows:
(1) gets Radix Rhodiolae, add 40～80% ethanol and extract, filter at 60～70 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract; Perhaps get Radix Rhodiolae, add water 10-12 and doubly measure decoction 1-3 time, each 1-3 hour, filter, merging filtrate, at 70-80 ℃, reclaim under reduced pressure adds ethanol to containing alcohol amount 60-80% to containing 3-5g crude drug/ml under the-0.5--1.0MPa condition, static 20-30 hour, filter, at 70-80 ℃, decompression recycling ethanol is to there not being the alcohol flavor under the-0.5--1.0MPa condition, continue to be concentrated into and contain 4-6g crude drug/ml, add ethanol to containing alcohol amount 70-90%, add 15-25%NaOH and transfer pH to 7-9, static 20-30 hour, filter, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Paeoniae Rubra, decoct with water 1-3 time, each 1-3 hour, filter, merging filtrate, reclaim under reduced pressure is to an amount of, add ethanol to containing alcohol amount 60-80%, static spending the night filters, reclaim ethanol to there not being the alcohol flavor, add ethanol, add 15-25%NaOH and transfer pH to 7-9 to containing alcohol amount 70-90%, static spending the night filters, and reclaims ethanol, concentrating under reduced pressure gets Radix Paeoniae Rubra extract;
Perhaps, get Radix Rhodiolae, Radix Paeoniae Rubra, add water 10-12 and doubly measure decoction 1-3 time, each 1-3 hour, filter, merging filtrate is at 70-80 ℃, reclaim under reduced pressure is to containing 3-5g crude drug/ml under the-0.5-1.0MPa condition, add ethanol to containing alcohol amount 60-80%, static 20-30 hour, filter, at 70-80 ℃, decompression recycling ethanol is not distinguished the flavor of to there being alcohol under the-0.5--1.0MPa condition, continues to be concentrated into to contain 4-6g crude drug/ml, adds ethanol to containing alcohol amount 70-90%, add 15-20%NaOH and transfer pH to 7-9, static 20-30 hour, filter, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
Perhaps get Radix Rhodiolae, Radix Paeoniae Rubra, add 40～80% ethanol and extract, filter at 60～70 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
(3) get Radix Notoginseng, carry out percolation, make solvent, flood after 30-50 hour with 90-100% ethanol by percolation, speed with per minute 2～4ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use the 50-70% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(4) get above-mentioned Radix Rhodiolae extract, Radix Paeoniae Rubra extract or Radix Rhodiolae and mix, add the dissolving of injection water, leave standstill, filter with the extract and the Radix Notoginseng extract of Radix Paeoniae Rubra, the filtrate ultrafiltration, the embedding ampoule promptly gets injection; Perhaps add 0.5-2.0% freeze-dried powder proppant, make its dissolving, aseptic subpackaged in the sterilization cillin bottle, lyophilization, tamponade, gland promptly gets lyophilized injectable powder; Perhaps add conventional medical accessory, make other any dosage form; In the preparation of described any dosage form, the content sum of effective ingredient Radix Notoginseng total arasaponins, Radix Paeoniae Rubra glycosides, rhodioside is greater than 25%.
Technical problem to be solved by this invention can also further realize by following technical scheme.The present invention is a kind of preparation method for the treatment of the medicine of coronary heart disease or coronary heart disease merging cardiac insufficiency, is characterized in that its step is as follows:
(1) gets Radix Rhodiolae, add 40～80% ethanol and extract, filter at 60～70 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract; Perhaps get Radix Rhodiolae, add water 10-12 and doubly measure decoction 1-3 time, each 1-3 hour, filter, merging filtrate, at 70-80 ℃, reclaim under reduced pressure adds ethanol to containing alcohol amount 60-80% to containing 3-5g crude drug/ml under the-0.5--1.0MPa condition, static 20-30 hour, filter, at 70-80 ℃, decompression recycling ethanol is to there not being the alcohol flavor under the-0.5--1.0MPa condition, continue to be concentrated into and contain 4-6g crude drug/ml, add ethanol to containing alcohol amount 70-90%, add 15-25%NaOH and transfer pH to 7-9, static 20-30 hour, filter, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Paeoniae Rubra, decoct with water 1-3 time, each 1-3 hour, filter, merging filtrate, reclaim under reduced pressure is to an amount of, add ethanol to containing alcohol amount 60-80%, static spending the night filters, reclaim ethanol to there not being the alcohol flavor, add ethanol, add 15-25%NaOH and transfer pH to 7-9 to containing alcohol amount 70-90%, static spending the night filters, and reclaims ethanol, concentrating under reduced pressure gets Radix Paeoniae Rubra extract;
Perhaps, get Radix Rhodiolae, Radix Paeoniae Rubra, add water 10-12 and doubly measure decoction 1-3 time, each 1-3 hour, filter, merging filtrate is at 70-80 ℃, reclaim under reduced pressure is to containing 3-5g crude drug/ml under the-0.5-1.0MPa condition, add ethanol to containing alcohol amount 60-80%, static 20-30 hour, filter, at 70-80 ℃, decompression recycling ethanol is not distinguished the flavor of to there being alcohol under the-0.5-1.0MPa condition, continues to be concentrated into to contain 4-6g crude drug/ml, adds ethanol to containing alcohol amount 70-90%, add 15-20%NaOH and transfer pH to 7-9, static 20-30 hour, filter, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
Perhaps get Radix Rhodiolae, Radix Paeoniae Rubra, add 40～80% ethanol and extract, filter at 60～70 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
(3) get Radix Notoginseng, carry out percolation, make solvent, flood after 30-50 hour with 90-100% ethanol by percolation, speed with per minute 2～4ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use the 50-70% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(4) get Folium Ginkgo, add 60-80% alcoholic solution that 8-12 doubly measures under 70～80 ℃ of temperature, warm macerating 1-3 hour, filter, add the 60-80% alcoholic solution that 6-10 doubly measures, under 70～80 ℃ of temperature, warm macerating 1-3 hour, filter, merging filtrate, recovery ethanol is not to there being the alcohol flavor, and thin up is to 2-4 times of original volume, static 20-30 hour, filter, filtrate is doubly measured absorption with macroporous adsorbent resin by 1-3, and is colourless to effluent with distilled water flushing, with the 70-90% ethanol elution, collect eluent, reclaim ethanol, drying under reduced pressure is to doing, refining, get the Folium Ginkgo extract powder;
(5) get extract, Radix Notoginseng extract and the Folium Ginkgo extract powder of Radix Rhodiolae extract, Radix Paeoniae Rubra extract or Radix Rhodiolae and Radix Paeoniae Rubra, mix, add solubilizing agent, add water for injection again and make its dissolving, leave standstill, filter, filtrate is with the ultrafiltration of 10000-30000 dalton filter membrane, and the embedding ampoule promptly gets injection; Or add 0.5-2.0% freeze-dried powder proppant, make dissolving, and additional water for injection, aseptic subpackaged in the sterilization cillin bottle, lyophilization, tamponade, gland promptly gets lyophilized injectable powder; Perhaps add conventional medical accessory, make other any dosage form; In the preparation of described any dosage form, the content sum of ketone was greater than 25% in effective ingredient Radix Notoginseng total arasaponins, Radix Paeoniae Rubra glycosides, rhodioside and Ginkgo total lactones were total.
In several above-mentioned preparation methoies, described raw material Radix Rhodiolae, Radix Notoginseng etc. can be pulverized and be coarse powder, also can be other forms, but for the ease of extracting active ingredient wherein, and preferable methods is to be coarse powder with raw material pulverizing.Described freeze-dried powder proppant is conventional medical accessory, comprises glucose, mannitol etc.Described solubilizing agent is some solubilizing agents commonly used, as HYDROXYPROPYL BETA-CYCLODEXTRIN, meglumine etc.
Composition of medicine disclosed in this invention meets Chinese medical theory, and wherein, Radix Rhodiolae has tangible anti-hypoxia, cold resistance, fatigue-resisting function; Radix Paeoniae Rubra has body-temp. reducing, calmness, anticonvulsant action, simultaneously coronary artery and blood vessel is had dilating effect; Radix Notoginseng also has hemostasis, invigorates blood circulation, changes silt, effects such as easing pain and diminishing inflammation, anticoagulant, cerebral blood flow increasing amount except that having effect such as strengthening by means of tonics, blood fat reducing blood glucose; Folium Ginkgo has effects such as blood pressure lowering blood fat.In three kinds of prescriptions of the present invention, each Chinese herbal medicine of distinguishing the flavor of complements each other, and can bring into play effect separately, also can promote the performance of the drug action of other medicines simultaneously, is a kind of treatment coronary heart disease of novel composing prescription or the efficient medicine that coronary heart disease merges cardiac insufficiency.
Compared with prior art, medicine of the present invention and preparation method thereof has the following advantages: one, medicine of the present invention is the medicine that a kind of new treatment coronary heart disease or coronary heart disease merge cardiac insufficiency, and it has advantages such as curative effect height, instant effect, side effect are little; Two, in preparation technology, adopt technologies such as secondary precipitate with ethanol and macroporous resin adsorption, extract obtained extractum active constituent content height; Three, can be made into true solution after extractum is dissolved in water, be different from the solution of general cosolvent preparation, this extract reclaims and concentrates and all carries out under reduced pressure simultaneously, and heated time is short, keeps its effective ingredient constant, has increased the homogeneity and the stability of preparation; Four, medicinal liquid has been removed the impurity that macromole in the former extract, pyrogen, microgranule, microorganism etc. have a strong impact on the injection safety, the safety that has improved preparation significantly through ultrafiltration.
The specific embodiment
The following examples are for allowing those skilled in the art understand the present invention further, rather than to the restriction of right of the present invention.
Embodiment 1.A kind ofly treat the medicine that coronary heart disease or coronary heart disease merge cardiac insufficiency, it is to be the medicament that raw material is made by Radix Rhodiolae and Radix Notoginseng, and wherein, the weight proportion of raw material is,
200 kilograms of 200 kilograms of Radix Notoginseng of Radix Rhodiolae
With the method for routine, it is added water decoct, extract active ingredient wherein, add conventional medical accessory, make oral liquid.
Embodiment 2.A kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency, wherein the weight proportion of each raw material is,
200 kilograms of 400 kilograms of Radix Notoginseng of Radix Rhodiolae
Its preparation method is as follows,
(1) get Radix Rhodiolae, 50% ethanol that adds 6 times of amounts extracts at 60 ℃ of warm macerating, filters, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Notoginseng, carry out percolation, make solvent, flood after 30 hours with 90% ethanol by percolation, speed with per minute 2ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 50% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(3) get above-mentioned Radix Rhodiolae extract and Radix Notoginseng extract and mix, add the dissolving of injection water, leave standstill, filter, the filtrate ultrafiltration, the embedding ampoule promptly gets injection.
Embodiment 3.A kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency, wherein the weight proportion of each raw material is,
300 kilograms of 200 kilograms of Radix Notoginseng of Radix Rhodiolae
Its preparation method is as follows,
(1) gets Radix Rhodiolae, be ground into coarse powder, add 10 times of amounts of water and decoct each 2 hours 2 times, filter, merging filtrate, at 70 ℃, reclaim under reduced pressure is to containing 3g crude drug/ml under the-0.5MPa condition, add ethanol to containing alcohol amount 60%, static 20 hours, filter, at 70 ℃, decompression recycling ethanol is not distinguished the flavor of to there being alcohol under the-0.5MPa condition, continues to be concentrated into to contain 4g crude drug/ml, adds ethanol to containing alcohol amount 70%, add 15%NaOH and transfer pH to 7, static 20 hours, filter, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Notoginseng, carry out percolation, make solvent, flood after 40 hours with 95% ethanol by percolation, speed with per minute 3ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 60% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(3) get above-mentioned Radix Rhodiolae extract and Radix Notoginseng extract and mix, add 0.5% freeze-dried powder proppant glucose, make its dissolving, aseptic subpackaged in the sterilization cillin bottle, lyophilization, tamponade, gland promptly gets lyophilized injectable powder; Wherein the content sum of effective ingredient Radix Notoginseng total arasaponins and rhodioside is 28%.
Embodiment 4.A kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency, wherein the weight proportion of each raw material is,
350 kilograms of Radix Notoginseng double centners of Radix Rhodiolae
Its preparation method is as follows,
(1) gets Radix Rhodiolae, be ground into coarse powder, add 12 times of amounts of water and decoct each 3 hours 3 times, filter, merging filtrate, at 80 ℃, reclaim under reduced pressure is to containing 4g crude drug/ml under the-0.8MPa condition, add ethanol to containing alcohol amount 80%, static 24 hours, filter, at 80 ℃, decompression recycling ethanol is not distinguished the flavor of to there being alcohol under the-0.8MPa condition, continues to be concentrated into to contain 5g crude drug/ml, adds ethanol to containing alcohol amount 90%, add 25%NaOH and transfer pH to 8, static 24 hours, filter, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Notoginseng, carry out percolation, make solvent, flood after 50 hours with 99% ethanol by percolation, speed with per minute 4ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 70% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(3) get above-mentioned Radix Rhodiolae extract and Radix Notoginseng extract and mix, add conventional medical accessory, make tablet.
Embodiment 5.A kind ofly treat the medicine that coronary heart disease or coronary heart disease merge cardiac insufficiency, it is to be the medicament that raw material is made by Radix Rhodiolae, Radix Notoginseng and Radix Paeoniae Rubra, and wherein the weight proportion of each raw material is,
200 kilograms of 200 kilograms of Radix Paeoniae Rubra of 200 kilograms of Radix Notoginseng of Radix Rhodiolae
Its preparation method is as follows,
(1) get Radix Rhodiolae, be ground into coarse powder, 60% ethanol that adds 8 times of amounts extracts at 65 ℃ of warm macerating, filter, decompression recycling ethanol concentrates, thin up leaves standstill, and filters, filtrate is by absorption with macroporous adsorbent resin, and is colourless to effluent with distilled water flushing, uses the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Paeoniae Rubra, be ground into coarse powder, add 10 times of amounts of water and decoct 2 times, each 3 hours, filter merging filtrate, reclaim under reduced pressure adds ethanol to containing alcohol amount 70%, static spending the night to an amount of, filter, reclaim ethanol, add ethanol to containing alcohol amount 75% to there not being the alcohol flavor, add 15%NaOH and transfer pH to 9, static spending the night filters, reclaim ethanol, concentrating under reduced pressure gets Radix Paeoniae Rubra extract;
(3) get Radix Notoginseng, carry out percolation, make solvent, flood after 45 hours with 100% ethanol by percolation, speed with per minute 3ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 65% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(4) get above-mentioned Radix Rhodiolae extract, Radix Paeoniae Rubra extract and Radix Notoginseng extract and mix, add the dissolving of injection water, leave standstill, filter, the filtrate ultrafiltration, the embedding ampoule promptly gets injection.
Embodiment 6.A kind ofly treat the medicine that coronary heart disease or coronary heart disease merge cardiac insufficiency, it is to be the medicament that raw material is made by Radix Rhodiolae, Radix Notoginseng and Radix Paeoniae Rubra, and wherein the weight proportion of each raw material is,
200 kilograms of Radix Paeoniae Rubra double centners of 400 kilograms of Radix Notoginseng of Radix Rhodiolae
Its preparation method is as follows,
(1) gets Radix Rhodiolae, be ground into coarse powder, add 11 times of amounts of water and decoct each 2 hours 3 times, filter, merging filtrate, at 75 ℃, reclaim under reduced pressure is to containing 5g crude drug/ml under the-1.0MPa condition, add ethanol to containing alcohol amount 75%, static 30 hours, filter, at 75 ℃, decompression recycling ethanol is not distinguished the flavor of to there being alcohol under the-1.0MPa condition, continues to be concentrated into to contain 6g crude drug/ml, adds ethanol to containing alcohol amount 85%, add 20%NaOH and transfer pH to 8, static 30 hours, filter, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Paeoniae Rubra, be ground into coarse powder, add 8 times of amounts of water and decoct 3 times, each 1 hour, filter merging filtrate, reclaim under reduced pressure adds ethanol to containing alcohol amount 70%, static spending the night to an amount of, filter, reclaim ethanol, add ethanol to containing alcohol amount 75% to there not being the alcohol flavor, add 25%NaOH and transfer pH to 8, static spending the night filters, reclaim ethanol, concentrating under reduced pressure gets Radix Paeoniae Rubra extract;
(3) get Radix Notoginseng, carry out percolation, make solvent, flood after 35 hours with 90% ethanol by percolation, speed with per minute 4ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 60% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(4) getting above-mentioned Radix Rhodiolae extract, Radix Paeoniae Rubra extract and Radix Notoginseng extract mixes, add 1.0% freeze-dried powder proppant mannitol, make its dissolving, aseptic subpackaged in the sterilization cillin bottle, lyophilization, tamponade, gland, promptly get lyophilized injectable powder, wherein the content sum of effective ingredient Radix Notoginseng total arasaponins, Radix Paeoniae Rubra glycosides, rhodioside is 30%.
Embodiment 7.A kind ofly treat the medicine that coronary heart disease or coronary heart disease merge cardiac insufficiency, it is to be the medicament that raw material is made by Radix Rhodiolae, Radix Notoginseng and Radix Paeoniae Rubra, and wherein the weight proportion of each raw material is,
400 kilograms of 300 kilograms of Radix Notoginseng double centners of Radix Rhodiolae Radix Paeoniae Rubra
Its preparation method is as follows,
(1) gets Radix Rhodiolae, Radix Paeoniae Rubra, add 12 times of amounts of water and decoct 3 times, each 3 hours, filter, merging filtrate, at 80 ℃, reclaim under reduced pressure adds ethanol to containing alcohol amount 480% to containing 4g crude drug/ml under the-0.8MPa condition, static 24 hours, filter, at 80 ℃, decompression recycling ethanol is not distinguished the flavor of to there being alcohol under the-0.8MPa condition, continues to be concentrated into to contain 5g crude drug/ml, adds ethanol to containing alcohol amount 70%, add 15%NaOH and transfer pH to 8, static 24 hours, filter, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
(2) get Radix Notoginseng, carry out percolation, make solvent, flood after 40 hours with 90% ethanol by percolation, speed with per minute 2ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 50% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(3) get above-mentioned Radix Rhodiolae and mix, add conventional medical accessory, make capsule with the extract and the Radix Notoginseng extract of Radix Paeoniae Rubra.
Embodiment 8.A kind ofly treat the medicine that coronary heart disease or coronary heart disease merge cardiac insufficiency, it is to be the medicament that raw material is made by Radix Rhodiolae, Radix Notoginseng, Radix Paeoniae Rubra and Folium Ginkgo, and wherein the weight proportion of each raw material is,
200 kilograms of 200 kilograms of Radix Notoginseng of Radix Rhodiolae
200 kilograms of Folium Ginkgo double centners of Radix Paeoniae Rubra
Its preparation method is as follows,
(1) gets Radix Rhodiolae, Radix Paeoniae Rubra, add 12 times of amounts of water and decoct 3 times, each 3 hours, filter, merging filtrate, at 80 ℃, reclaim under reduced pressure adds ethanol to containing alcohol amount 480% to containing 4g crude drug/ml under the-0.8MPa condition, static 24 hours, filter, at 80 ℃, decompression recycling ethanol is not distinguished the flavor of to there being alcohol under the-0.8MPa condition, continues to be concentrated into to contain 5g crude drug/ml, adds ethanol to containing alcohol amount 70%, add 15%NaOH and transfer pH to 8, static 24 hours, filter, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
(2) get Radix Notoginseng, carry out percolation, make solvent, flood after 40 hours with 90% ethanol by percolation, speed with per minute 2ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 50% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(3) get Folium Ginkgo, 60% alcoholic solution that adds 10 times of amounts under 70 ℃ of temperature, warm macerating 3 hours, filter, 60% alcoholic solution that adds 10 times of amounts, under 70 ℃ of temperature, warm macerating 1 hour, filter, merging filtrate reclaims ethanol to there not being the alcohol flavor, and thin up is to 3 times of original volume, static 24 hours, filter, filtrate is by 2 times of amount absorption with macroporous adsorbent resin, and is colourless to effluent with distilled water flushing, with 70% ethanol elution, collect eluent, reclaim ethanol, drying under reduced pressure is to doing, refining, get the Folium Ginkgo extract powder;
(4) get extract, Radix Notoginseng extract and the Folium Ginkgo extract powder of Radix Rhodiolae and Radix Paeoniae Rubra, mix, add the solubilizing agent HYDROXYPROPYL BETA-CYCLODEXTRIN, add water for injection again and make its dissolving, leave standstill, filter, filtrate is with 30000 dalton's filter membrane ultrafiltration, and the embedding ampoule promptly gets injection.
Embodiment 9.A kind ofly treat the medicine that coronary heart disease or coronary heart disease merge cardiac insufficiency, it is to be the medicament that raw material is made by Radix Rhodiolae, Radix Notoginseng, Radix Paeoniae Rubra and Folium Ginkgo, and wherein the weight proportion of each raw material is,
300 kilograms of 400 kilograms of Radix Notoginseng of Radix Rhodiolae
300 kilograms of 200 kilograms of Folium Ginkgos of Radix Paeoniae Rubra
Its preparation method is as follows,
(1) gets Radix Rhodiolae, be ground into coarse powder, add 11 times of amounts of water and decoct each 2 hours 3 times, filter, merging filtrate, at 75 ℃, reclaim under reduced pressure is to containing 5g crude drug/ml under the-1.0MPa condition, add ethanol to containing alcohol amount 75%, static 30 hours, filter, at 75 ℃, decompression recycling ethanol is not distinguished the flavor of to there being alcohol under the-1.0MPa condition, continues to be concentrated into to contain 6g crude drug/ml, adds ethanol to containing alcohol amount 85%, add 20%NaOH and transfer pH to 8, static 30 hours, filter, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Paeoniae Rubra, be ground into coarse powder, add 8 times of amounts of water and decoct 3 times, each 1 hour, filter merging filtrate, reclaim under reduced pressure adds ethanol to containing alcohol amount 70%, static spending the night to an amount of, filter, reclaim ethanol, add ethanol to containing alcohol amount 75% to there not being the alcohol flavor, add 25%NaOH and transfer pH to 8, static spending the night filters, reclaim ethanol, concentrating under reduced pressure gets Radix Paeoniae Rubra extract;
(3) get Radix Notoginseng, carry out percolation, make solvent, flood after 35 hours with 90% ethanol by percolation, speed with per minute 4ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 60% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(4) get Folium Ginkgo, 80% alcoholic solution that adds 8 times of amounts under 80 ℃ of temperature, warm macerating 3 hours, filter, 80% alcoholic solution that adds 6 times of amounts, under 80 ℃ of temperature, warm macerating 2 hours, filter, merging filtrate reclaims ethanol to there not being the alcohol flavor, and thin up is to 2 times of original volume, static 30 hours, filter, filtrate is by 3 times of amount absorption with macroporous adsorbent resin, and is colourless to effluent with distilled water flushing, with 90% ethanol elution, collect eluent, reclaim ethanol, drying under reduced pressure is to doing, with active carbon dephenolize acid treating, get the Folium Ginkgo extract powder;
(5) get Radix Rhodiolae extract, Radix Paeoniae Rubra extract, Radix Notoginseng extract and Folium Ginkgo extract powder, mix, add 2.0% freeze-dried powder proppant glucose, make dissolving, and additional water for injection, aseptic subpackaged in the sterilization cillin bottle, lyophilization, tamponade, gland promptly gets lyophilized injectable powder.
Embodiment 10.A kind ofly treat the medicine that coronary heart disease or coronary heart disease merge cardiac insufficiency, it is to be the medicament that raw material is made by Radix Rhodiolae, Radix Notoginseng, Radix Paeoniae Rubra and Folium Ginkgo, and wherein the weight proportion of each raw material is,
400 kilograms of 300 kilograms of Radix Notoginseng of Radix Rhodiolae
200 kilograms of 400 kilograms of Folium Ginkgos of Radix Paeoniae Rubra
Its preparation method is as follows,
(1) get Radix Rhodiolae, be ground into coarse powder, 60% ethanol that adds 8 times of amounts extracts at 65 ℃ of warm macerating, filter, decompression recycling ethanol concentrates, thin up leaves standstill, and filters, filtrate is by absorption with macroporous adsorbent resin, and is colourless to effluent with distilled water flushing, uses the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Paeoniae Rubra, be ground into coarse powder, add 10 times of amounts of water and decoct 2 times, each 3 hours, filter merging filtrate, reclaim under reduced pressure adds ethanol to containing alcohol amount 70%, static spending the night to an amount of, filter, reclaim ethanol, add ethanol to containing alcohol amount 75% to there not being the alcohol flavor, add 15%NaOH and transfer pH to 9, static spending the night filters, reclaim ethanol, concentrating under reduced pressure gets Radix Paeoniae Rubra extract;
(3) get Radix Notoginseng, carry out percolation, make solvent, flood after 45 hours with 100% ethanol by percolation, speed with per minute 3ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 65% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(4) get Folium Ginkgo, 70% alcoholic solution that adds 8 times of amounts is under 75 ℃ of temperature, and warm macerating 3 hours filters, 70% alcoholic solution that adds 8 times of amounts, under 75 ℃ of temperature, warm macerating 3 hours filters, merging filtrate reclaims ethanol to there not being the alcohol flavor, and thin up is to 3 times of original volume, static 20 hours, filter, filtrate is by 2 times of amount absorption with macroporous adsorbent resin, and is colourless to effluent with distilled water flushing, with 80% ethanol elution, collect eluent, reclaim ethanol, drying under reduced pressure is to doing, and is refining, gets the Folium Ginkgo extract powder;
(5) get Radix Rhodiolae extract, Radix Paeoniae Rubra extract, Radix Notoginseng extract and Folium Ginkgo extract powder, mix, add conventional medical accessory, make granule.
Embodiment 11.A kind ofly treat the medicine that coronary heart disease or coronary heart disease merge cardiac insufficiency, it is to be the medicament that raw material is made by Radix Rhodiolae, Radix Notoginseng and Radix Paeoniae Rubra, and wherein the weight proportion of each raw material is,
150 kilograms of 300 kilograms of Radix Paeoniae Rubra of 400 kilograms of Radix Notoginseng of Radix Rhodiolae
Its preparation method is as follows,
(1) gets Radix Rhodiolae, Radix Paeoniae Rubra, add 50% ethanol and extract, filter at 65 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
(2) get Radix Notoginseng, carry out percolation, make solvent, flood after 45 hours with 90% ethanol by percolation, speed with per minute 3.5ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 50% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(3) get above-mentioned Radix Rhodiolae and mix, add conventional medical accessory, make capsule with the extract and the Radix Notoginseng extract of Radix Paeoniae Rubra.
Embodiment 12.A kind ofly treat the medicine that coronary heart disease or coronary heart disease merge cardiac insufficiency, it is to be the medicament that raw material is made by Radix Rhodiolae, Radix Notoginseng, Radix Paeoniae Rubra and Folium Ginkgo, and wherein the weight proportion of each raw material is,
300 kilograms of 350 kilograms of Radix Notoginseng of Radix Rhodiolae
250 kilograms of 350 kilograms of Folium Ginkgos of Radix Paeoniae Rubra
Its preparation method is as follows,
(1) gets Radix Rhodiolae, Radix Paeoniae Rubra, add 70% ethanol and extract, filter at 60 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
(2) get Radix Notoginseng, carry out percolation, make solvent, flood after 38 hours with 90% ethanol by percolation, speed with per minute 2.5ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use 50% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(4) get Folium Ginkgo, 70% alcoholic solution that adds 9 times of amounts under 80 ℃ of temperature, warm macerating 3 hours, filter, 70% alcoholic solution that adds 10 times of amounts, under 80 ℃ of temperature, warm macerating 3 hours, filter, merging filtrate reclaims ethanol to there not being the alcohol flavor, and thin up is to 4 times of original volume, static 28 hours, filter, filtrate is by 2 times of amount absorption with macroporous adsorbent resin, and is colourless to effluent with distilled water flushing, with 80% ethanol elution, collect eluent, reclaim ethanol, drying under reduced pressure is to doing, refining, get the Folium Ginkgo extract powder;
(5) get extract, Radix Notoginseng extract and the Folium Ginkgo extract powder of above-mentioned Radix Rhodiolae and Radix Paeoniae Rubra, mix, add conventional medical accessory, make oral liquid.
Embodiment 13.The selection of freeze-dried powder adjuvant.
Get Radix Rhodiolae, Radix Paeoniae Rubra, Radix Notoginseng,, get extracts such as Radix Rhodiolae (calculating), add proper amount of water for injection, contain the 10g crude drug with every bottle and make the Radix Rhodiolae freeze-dried powder with a recipe quantity by technological operation.The according to the form below ratio adds mannitol, glucose, and by every bottle of 6ml volume dosing medicinal liquid, packing after the ultrafiltration, mouldability is observed in lyophilizing (earlier-15 ℃ freezing 2 hours, ℃ pre-freeze 3 hours again-40 after waiting to freeze was put in the small frozen exsiccator lyophilizing 48 hours), sees Table 1.
Table 1 different auxiliary material amount is to the mouldability impact analysis
Adjuvant type adjuvant amount
The preparation character
(mg/ bottle)
30 powder pin molding are poor slightly, do not have obviously caking, and block is loose, hole
Greatly, color and luster is inhomogeneous.
Mannitol 60 powder pin molding are good, do not have obviously caking, and sedimentation is loose, hole,
Color and luster is even.
During 90 powder pin molding, obvious caking is arranged, part has the atrophy shape, has big
Crazing, freeze-drying time obviously prolongs.
30 powder pin molding are poor slightly, do not have obviously caking, and block is loose but uneven
Even, hole is big, and freeze-drying time is long.
Glucose 60 powder pin molding are bad slightly, do not have obviously caking, and certain sedimentation is arranged,
Hole, color and luster are inhomogeneous, and freeze-drying time is long.
90 powder pin molding are bad, do not have obviously caking, and are loose, but freeze-drying time
Prolong.
As known from Table 1, add not commensurability adjuvant, to the difference that influences of mouldability, the mannitol consumption is when 60mg/6ml, and the molding of powder pin is good, does not have obviously caking, and sedimentation is loose, and hole, color and luster are even, so the mannitol consumption is 60mg/6ml.
Embodiment 14.The selection of ultrafiltration condition.
Selecting rolling ultrafiltration (polysulfone membrane) film main part for use, adopt the film of different molecular weight, serves as to investigate index with the content of rhodioside, arasaponin, Radix Paeoniae Rubra glycosides in the medicinal liquid, each test specimen is carried out the different molecular weight ultrafilter membrane preferably compare, and calculates the relative rate of transform.Experimental result sees Table 2.
Table 2 ultrafiltration test result analysis
Tested number membrane molecule amount arasaponin Rg1+Rb1 rate of transform % Radix Paeoniae Rubra glycosides rate of transform % rhodioside rate of transform %
1 10000 69.23 87.67 85.70
2 30000 85.61 91.23 93.22
As shown in Table 2, different aperture film has in various degree the influence of holding back to the Radix Rhodiolae composition.The membrane molecule amount is more little, and the rate of transform of Radix Rhodiolae composition correspondingly reduces, and 10000 molecular weight films are bigger than membrane molecule amount 30000 to the rate of transform influence of its rhodioside, arasaponin, Radix Paeoniae Rubra glycosides.Can remove pyrogen, antibacterial etc. during ultrafiltration, after this safety, particularly injectable powder that improve injection can not carry out disinfection again, and the ultrafiltration technology process is particularly important, and therefore selecting for use the less molecular weight of composition influence is that 30000 film carries out ultrafiltration.
Embodiment 15.Injection and the test of injectable powder pyrogen test
Choose healthy rabbits by the check of Chinese Pharmacopoeia appendix XIII A method, measure each rabbit anus body temperature, result such as table 3 with MC-3B computer numeral formula clinical thermometer.
The result of the test of table 3 pyrogen test
Body temperature after the administration of administration precursor (℃) fervescence
The high fever of administration group ℃
Temperature (℃) 1 hour 2 hours 3 hours sums (℃)
38.3 38.4 38.5 38.6 38.6 0.3
Ultrafiltration
38.4 38.4 38.4 38.5 38.5 0.1
The powder pin
39.2 39.4 39.5 39.5 39.5 0.3
38.2 38.6 39.0 39.4 39.4 1.2
Not ultrafiltration
38.6 38.9 39.7 38.9 39.7 1.1
The powder pin
38.4 39.6 39.3 39.7 39.7 1.3
The result of the test of table 4 pyrogen test
Body temperature after the administration of administration precursor (℃) fervescence
The high fever of administration group ℃
Temperature (℃) 1 hour 2 hours 3 hours sums (℃)
38.5 38.5 38.6 38.7 38.7 0.2
Ultrafiltration
38.4 38.4 38.4 38.5 38.6 0.1
Injection
39.1 39.3 39.5 39.8 39.5 0.4
38.0 38.2 38.5 38.9 38.9 0.9
Not ultrafiltration
38.3 38.9 39.9 39.98 39.1 0.8
Injection
38.6 39.6 39.3 39.7 39.7 1.1
By table 4 as seen, the rabbit injection is through the powder pin of ultrafiltration, and fervescence is not obvious; And injection is without the powder pin of ultrafiltration, and body temperature slightly raises; The result shows that solution can be removed pyrogen through ultrafiltration, guarantees the safety of preparation.
Claims (10)
1, a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency is characterized in that it is to comprise that Radix Rhodiolae and Radix Notoginseng are the medicament that raw material is made.
2, a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency according to claim 1 is characterized in that wherein the weight proportion of each raw material is,
Radix Rhodiolae 100-400 Radix Notoginseng 100-400
3, a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency according to claim 1 is characterized in that, also comprises Radix Paeoniae Rubra in its raw material.
4, a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency according to claim 3 is characterized in that wherein the weight proportion of each raw material is,
Radix Rhodiolae 100-400 Radix Notoginseng 100-400 Radix Paeoniae Rubra 100-400
5, a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency according to claim 3 is characterized in that, also comprises Folium Ginkgo in its raw material.
6, a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency according to claim 5 is characterized in that wherein the weight proportion of each raw material is,
Radix Rhodiolae 100-400 Radix Notoginseng 100-400
Radix Paeoniae Rubra 100-400 Folium Ginkgo 100-400
7, according to any one described a kind of medicine for the treatment of coronary heart disease or coronary heart disease merging cardiac insufficiency among the claim 1-6, it is characterized in that described medicament is said any dosage form on the pharmaceutics.
8, claim 1 or 2 described a kind of coronary heart disease or coronary heart disease for the treatment of merge cardiac insufficiency
The preparation method of medicine is characterized in that, its step is as follows:
(1) gets Radix Rhodiolae, add 40～80% ethanol and extract, filter at 60～70 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
Perhaps get Radix Rhodiolae, add water 10-12 and doubly measure decoction 1-3 time, each 1-3 hour, filter, merging filtrate, at 70-80 ℃, reclaim under reduced pressure adds ethanol to containing alcohol amount 60-80% to containing 3-5g crude drug/ml under the-0.5--1.0MPa condition, static 20-30 hour, filter, at 70-80 ℃, decompression recycling ethanol is to there not being the alcohol flavor under the-0.5--1.0MPa condition, continue to be concentrated into and contain 4-6g crude drug/ml, add ethanol to containing alcohol amount 70-90%, add 15-25%NaOH and transfer pH to 7-9, static 20-30 hour, filter, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Notoginseng, carry out percolation, make solvent, flood after 30-50 hour with 90-100% ethanol by percolation, speed with per minute 2～4ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use the 50-70% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(3) get above-mentioned Radix Rhodiolae extract and Radix Notoginseng extract and mix, add the dissolving of injection water, leave standstill, filter, the filtrate ultrafiltration, the embedding ampoule promptly gets injection; Perhaps add 0.5-2.0% freeze-dried powder proppant, make its dissolving, aseptic subpackaged in the sterilization cillin bottle, lyophilization, tamponade, gland promptly gets lyophilized injectable powder; Perhaps add conventional medical accessory, make other any dosage form according to a conventional method, in the preparation of described any dosage form, the content sum of effective ingredient Radix Notoginseng total arasaponins and rhodioside is greater than 25%.
9, claim 3 or 4 described a kind of preparation methoies for the treatment of the medicine of coronary heart disease or coronary heart disease merging cardiac insufficiency is characterized in that its step is as follows:
(1) gets Radix Rhodiolae, add 40～80% ethanol and extract, filter at 60～70 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract; Perhaps get Radix Rhodiolae, add water 10-12 and doubly measure decoction 1-3 time, each 1-3 hour, filter, merging filtrate, at 70-80 ℃, reclaim under reduced pressure adds ethanol to containing alcohol amount 60-80% to containing 3-5g crude drug/ml under the-0.5--1.0MPa condition, static 20-30 hour, filter, at 70-80 ℃, decompression recycling ethanol is to there not being the alcohol flavor under the-0.5--1.0MPa condition, continue to be concentrated into and contain 4-6g crude drug/ml, add ethanol to containing alcohol amount 70-90%, add 15-25%NaOH and transfer pH to 7-9, static 20-30 hour, filter, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Paeoniae Rubra, decoct with water 1-3 time, each 1-3 hour, filter, merging filtrate, reclaim under reduced pressure is to an amount of, add ethanol to containing alcohol amount 60-80%, static spending the night filters, reclaim ethanol to there not being the alcohol flavor, add ethanol, add 15-25%NaOH and transfer pH to 7-9 to containing alcohol amount 70-90%, static spending the night filters, and reclaims ethanol, concentrating under reduced pressure gets Radix Paeoniae Rubra extract;
Perhaps, get Radix Rhodiolae, Radix Paeoniae Rubra, add water 10-12 and doubly measure decoction 1-3 time, each 1-3 hour, filter, merging filtrate is at 70-80 ℃, reclaim under reduced pressure is to containing 3-5g crude drug/ml under the-0.5-1.0MPa condition, add ethanol to containing alcohol amount 60-80%, static 20-30 hour, filter, at 70-80 ℃, decompression recycling ethanol is not distinguished the flavor of to there being alcohol under the-0.5--1.0MPa condition, continues to be concentrated into to contain 4-6g crude drug/ml, adds ethanol to containing alcohol amount 70-90%, add 15-20%NaOH and transfer pH to 7-9, static 20-30 hour, filter, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
Perhaps get Radix Rhodiolae, Radix Paeoniae Rubra, add 40～80% ethanol and extract, filter at 60～70 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
(3) get Radix Notoginseng, carry out percolation, make solvent, flood after 30-50 hour with 90-100% ethanol by percolation, speed with per minute 2～4ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use the 50-70% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(4) get above-mentioned Radix Rhodiolae extract, Radix Paeoniae Rubra extract or Radix Rhodiolae and mix, add the dissolving of injection water, leave standstill, filter with the extract and the Radix Notoginseng extract of Radix Paeoniae Rubra, the filtrate ultrafiltration, the embedding ampoule promptly gets injection; Perhaps add 0.5-2.0% freeze-dried powder proppant, make its dissolving, aseptic subpackaged in the sterilization cillin bottle, lyophilization, tamponade, gland promptly gets lyophilized injectable powder; Perhaps add conventional medical accessory, make other any dosage form; In the preparation of described any dosage form, the content sum of effective ingredient Radix Notoginseng total arasaponins, Radix Paeoniae Rubra glycosides, rhodioside is greater than 25%.
10, claim 5 or 6 described a kind of preparation methoies for the treatment of the medicine of coronary heart disease or coronary heart disease merging cardiac insufficiency is characterized in that its step is as follows,
(1) gets Radix Rhodiolae, add 40～80% ethanol and extract, filter at 60～70 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract; Perhaps get Radix Rhodiolae, add water 10-12 and doubly measure decoction 1-3 time, each 1-3 hour, filter, merging filtrate, at 70-80 ℃, reclaim under reduced pressure adds ethanol to containing alcohol amount 60-80% to containing 3-5g crude drug/ml under the-0.5--1.0MPa condition, static 20-30 hour, filter, at 70-80 ℃, decompression recycling ethanol is to there not being the alcohol flavor under the-0.5-1.0MPa condition, continue to be concentrated into and contain 4-6g crude drug/ml, add ethanol to containing alcohol amount 70-90%, add 15-25%NaOH and transfer pH to 7-9, static 20-30 hour, filter, reclaim ethanol, concentrating under reduced pressure gets Radix Rhodiolae extract;
(2) get Radix Paeoniae Rubra, decoct with water 1-3 time, each 1-3 hour, filter, merging filtrate, reclaim under reduced pressure is to an amount of, add ethanol to containing alcohol amount 60-80%, static spending the night filters, reclaim ethanol to there not being the alcohol flavor, add ethanol, add 15-25%NaOH and transfer pH to 7-9 to containing alcohol amount 70-90%, static spending the night filters, and reclaims ethanol, concentrating under reduced pressure gets Radix Paeoniae Rubra extract;
Perhaps, get Radix Rhodiolae, Radix Paeoniae Rubra, add water 10-12 and doubly measure decoction 1-3 time, each 1-3 hour, filter, merging filtrate is at 70-80 ℃, reclaim under reduced pressure is to containing 3-5g crude drug/ml under the-0.5-1.0MPa condition, add ethanol to containing alcohol amount 60-80%, static 20-30 hour, filter, at 70-80 ℃, decompression recycling ethanol is not distinguished the flavor of to there being alcohol under the-0.5-1.0MPa condition, continues to be concentrated into to contain 4-6g crude drug/ml, adds ethanol to containing alcohol amount 70-90%, add 15-20%NaOH and transfer pH to 7-9, static 20-30 hour, filter, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
Perhaps get Radix Rhodiolae, Radix Paeoniae Rubra, add 40～80% ethanol and extract, filter at 60～70 ℃ of warm macerating, decompression recycling ethanol concentrates thin up, leave standstill, filter, filtrate is passed through absorption with macroporous adsorbent resin, colourless with distilled water flushing to effluent, use the Diluted Alcohol eluting, collect eluent, reclaim ethanol, concentrating under reduced pressure, the extract of Radix Rhodiolae and Radix Paeoniae Rubra;
(3) get Radix Notoginseng, carry out percolation, make solvent, flood after 30-50 hour with 90-100% ethanol by percolation, speed with per minute 2～4ml is carried out percolation, collects percolate to Radix Notoginseng total arasaponins and filters out fully, and percolate decolours through aluminium oxide, filter, reclaim ethanol and be concentrated into the thick paste shape, be dissolved in water, static cold preservation filters macroporous resin adsorption on the filtrate, use the 50-70% ethanol elution, collect eluent, reclaim ethanol, be concentrated into the thick paste shape, drying promptly gets Radix Notoginseng extract;
(4) get Folium Ginkgo, add 60-80% alcoholic solution that 8-12 doubly measures under 70～80 ℃ of temperature, warm macerating 1-3 hour, filter, add 60 80% alcoholic solution that 6-10 doubly measures, under 70～80 ℃ of temperature, warm macerating 1-3 hour, filter, merging filtrate, recovery ethanol is not to there being the alcohol flavor, and thin up is to 2-4 times of original volume, static 20-30 hour, filter, filtrate is doubly measured absorption with macroporous adsorbent resin by 1-3, and is colourless to effluent with distilled water flushing, with the 70-90% ethanol elution, collect eluent, reclaim ethanol, drying under reduced pressure is to doing, refining, get the Folium Ginkgo extract powder;
(5) get extract, Radix Notoginseng extract and the Folium Ginkgo extract powder of Radix Rhodiolae extract, Radix Paeoniae Rubra extract or Radix Rhodiolae and Radix Paeoniae Rubra, mix, add solubilizing agent, add water for injection again and make its dissolving, leave standstill, filter, filtrate is with the ultrafiltration of 10000-30000 dalton filter membrane, and the embedding ampoule promptly gets injection; Or add 0.5-2.0% freeze-dried powder proppant, make dissolving, and additional water for injection, aseptic subpackaged in the sterilization cillin bottle, lyophilization, tamponade, gland promptly gets lyophilized injectable powder; Perhaps add conventional medical accessory, make other any dosage form; In the preparation of described any dosage form, ketone contained sum greater than 25% in effective ingredient Radix Notoginseng total arasaponins, Radix Paeoniae Rubra glycosides, rhodioside and Ginkgo total lactones were total.
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| WO2007124674A1 (en) * | 2006-04-20 | 2007-11-08 | Integrated Chinese Medicine Holdings, Ltd. | Compositions for promoting brain and cardiovascular health, preventing and treating brain and cardiovascular disorders |
| CN100398117C (en) * | 2005-12-23 | 2008-07-02 | 关中宪 | Medicine for treating coronary heart disease |
| US7906159B2 (en) | 2008-04-17 | 2011-03-15 | Vivien Chou | Herbal compositions and methods for enhancing vital energy and athletic performance |
| CN102940694A (en) * | 2012-10-31 | 2013-02-27 | 成都医路康医学技术服务有限公司 | Medicine composition for treating cardia-cerebrovascular diseases |
| CN103497873A (en) * | 2013-09-11 | 2014-01-08 | 刘祥义 | Active rhizoma gastrodiae raw stock wine and preparation method thereof |
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2003
- 2003-08-26 CN CN 03152897 patent/CN1248698C/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100398117C (en) * | 2005-12-23 | 2008-07-02 | 关中宪 | Medicine for treating coronary heart disease |
| WO2007124674A1 (en) * | 2006-04-20 | 2007-11-08 | Integrated Chinese Medicine Holdings, Ltd. | Compositions for promoting brain and cardiovascular health, preventing and treating brain and cardiovascular disorders |
| US7906159B2 (en) | 2008-04-17 | 2011-03-15 | Vivien Chou | Herbal compositions and methods for enhancing vital energy and athletic performance |
| US8119170B2 (en) | 2008-04-17 | 2012-02-21 | Wen Hsien Chou | Herbal compositions and methods for enhancing vital energy and athletic performance |
| CN102940694A (en) * | 2012-10-31 | 2013-02-27 | 成都医路康医学技术服务有限公司 | Medicine composition for treating cardia-cerebrovascular diseases |
| CN102940694B (en) * | 2012-10-31 | 2014-06-25 | 成都医路康医学技术服务有限公司 | Medicine composition for treating cardia-cerebrovascular diseases |
| CN103497873A (en) * | 2013-09-11 | 2014-01-08 | 刘祥义 | Active rhizoma gastrodiae raw stock wine and preparation method thereof |
| CN103497873B (en) * | 2013-09-11 | 2016-05-11 | 刘祥义 | A kind of active Gastrodia elata original plasm wine and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN1248698C (en) | 2006-04-05 |
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