CN1390949A - Reagent kit for detecting different microbes with colloidal gold by one step - Google Patents
Reagent kit for detecting different microbes with colloidal gold by one step Download PDFInfo
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- CN1390949A CN1390949A CN 02112230 CN02112230A CN1390949A CN 1390949 A CN1390949 A CN 1390949A CN 02112230 CN02112230 CN 02112230 CN 02112230 A CN02112230 A CN 02112230A CN 1390949 A CN1390949 A CN 1390949A
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- colloidal gold
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Abstract
A reagent kit for detecting one or more microbes by colloidal gold in one step is composed of bottom plate, colloidal gold pad coated by the labelled colloidal gold for capturing one or more microbe antibodies, specimen absorbing pad, water absorbing pad and the reaction membrane, which is a nitrocellulose membrane and has one or more detecting strips for capturing the antigen (or antibody) of a microbe to be detected, and a reference strip coated with another antibody of said antigen (or antibody). Its advantages are high speed, low cost and easy operation.
Description
Technical field
The test kit that the Radioactive colloidal gold of the present invention's many inspections of one step detects microorganism relates to a kind of device of one or more microorganisms of rapid detection simultaneously, can be applicable to biology, medical science and association area.
Background technology
Immune colloidal gold technique in biomedical each research field, has particularly obtained increasingly extensive application as a kind of new immunological method in medical test.Radioactive colloidal gold has become after fluorescein, emitting isotope and enzyme, and a kind of on-radiation commonly used in immunolabelling technique is marked the track agent.Radioactive colloidal gold be by hydrochloro-auric acid at reductive agent, as tannic acid, trisodium citrate, effect under polymerization form the gold grain of specific size, and since electrostatic interaction become a kind of stable colloidal state, it has high electron density, and can with multiple biomacromolecule bonded characteristics.The immuno-gold labeling technology has mainly been utilized this feature of gold grain, be in the visible chocolate particle of microscopically in gold mark protein binding, when these mark things are assembled in a large number at corresponding part place, naked eyes red color visible or red-purple spot, thereby be used for qualitative or semiquantitative tachysynthesis detection method.
Traditional microorganism detection need be to bacterium or viral smear, cultivation, numeration detects after a few hours or a couple of days, and using monoclonal antibody is in conjunction with various forms of radioimmunoassays (RIA), enzyme immunoassay (EIA), fluorometric analysis (FIA), chemical analysis immunoluminescence (CIA), (BLIA) bioluminescence immunoassay (BIA) etc., be enough to detect the microbial antigen of trace in the clinical samples, so just, can remove bacterium or virus culture process from, directly finish the quick diagnosis of infected by microbes.
Since the test kit that detects microorganism with Radioactive colloidal gold with traditional bacterium or viral smear, cultivate to detect and compare, and detection method such as exempt from, enzyme is exempted from, fluorometric analysis, chemo-immunity are luminous and compare advantages such as having (step is detected and can finish) easy and simple to handle and (only need several minutes) fast with putting.So huge development potentiality is arranged aspect microorganism detection.
Existing microorganism detection is based on detection methods such as traditional bacterium or viral smear, cultivations, its weak point is, the loaded down with trivial details complexity of first operation, how need dye as smear method, culture method need dispose substratum and different bacteriums needs different substratum, cultivates by its required different culture condition then.These methods need a few hours or a couple of days to finish, and are consuming time longer.The second since different nacterial smear or cultivate need be through different dyestuffs painted, perhaps cultivate, so can not single job detect multiple microorganism simultaneously with different substratum and different environmental requirements.The 3rd, traditional method requires strict when operation, to avoid the microbiological contamination interference detection results.The 4th, for the diagnosis of some deep fungal infection, because the positive rate of cultivating from blood or body fluid is very low, so comparatively difficult with traditional technique in measuring.
Radioimmunology analysis (RIA), enzyme exempt to analyze (EIA), fluorometric analysis (FIA), chemical analysis immunoluminescence (CIA) the detections microorganism of etc.ing is compared traditional detection method and wants simple quick, but also need several hours, complicated operation, and the operator had relatively high expectations.And the test kit of correlation detection microorganism also can only carry out the individual event detection to microorganism.
The Radioactive colloidal gold that the purpose of this invention is to provide a kind of many inspections of a step detects the test kit of microorganism, and it can apace and can once detect one or more microorganisms.
Purpose of the present invention can realize by following technical proposal: the test kit that the Radioactive colloidal gold of many inspections of a step detects microorganism comprises base plate, Radioactive colloidal gold pad, absorption of sample pad, absorbent pad and reaction film, wherein, the described Radioactive colloidal gold pad antigen that is used to catch a kind of and more than one microorganisms to be checked (or antibody) of Radioactive colloidal gold that has been coated with mark; Described reaction film is a nitrocellulose filter, be fixed with one or one or more detection band (T band) on it, with contrast band (C band), every described detection with on be embedded with the antigen (or antibody) that can catch a kind of microorganism to be checked, contrast with on be embedded with the another kind of antibody (or two anti-) of anti-this antigen (or antibody).The present invention one goes on foot the test kit of the Radioactive colloidal golds detection microorganisms of many inspections, is to detect microorganism by antigen or the antibody that detects microorganism in the sample, the energy rapid detection, and can one or more microorganisms of step detection.
On the technique scheme basis, microorganism that can be detected can be: one or more bacterium of intestinal bacteria, streptococcus pneumoniae, helicobacter pylori, Mycobacterium tuberculosis, candidiasis, also can be mycoplasma pneumoniae, influenza virus, parainfluenza virus, one or more viruses of respiratory syncytial virus, or comprise bacterium and virus simultaneously.
Superiority of the present invention is: the Radioactive colloidal gold of this many inspections of a step detects the test kit of microorganism and compares with traditional detection, has advantage fast, only needs several minutes just can finish detection; Compare with traditional detection, and detection method such as exempt from, enzyme is exempted from, fluorometric analysis, chemo-immunity are luminous and compare and have advantages of simple operation with putting, a step is detected and can finish; Owing to have advantages of simple operation, therefore require lower to operator; Owing to save repeated operation and material, so its production cost will be lower than the cost of each individual event check-out console sum.
Description of drawings
The Radioactive colloidal gold of accompanying drawing many inspections of 1, one step detects the structural representation of the test kit of microorganism.
The Radioactive colloidal gold of accompanying drawing many inspections of 2, one step detects the schematic appearance of the test kit of microorganism.
Accompanying drawing 3, two positive test symbol synoptic diagram.
Embodiment
Referring to accompanying drawing 1, the test kit that the Radioactive colloidal golds of many inspections of one step of present embodiment detect microorganisms comprises base plate 11, be bonded at the nitrocellulose filter 12 that is embedded with T band and C band on the base plate, be covered in absorbent pad 13, the Radioactive colloidal gold pad 14 that is covered in the nitrocellulose filter lower end, the absorption of sample pad 15 of nitrocellulose filter upper end, and is covered in the absorption of sample pad on the Radioactive colloidal gold pad.
Be embedded with the antigen (or antibody) (T band) 16,17 that can catch two kinds of microorganisms to be checked respectively in the T region of nitrocellulose filter 12, the embedding of C region the antibody of anti-this antigen (or antibody) (or two anti-) 18.
Microorganism to be checked is two in the microorganisms such as intestinal bacteria, streptococcus pneumoniae, helicobacter pylori, Mycobacterium tuberculosis, candidiasis, mycoplasma pneumoniae, influenza virus, parainfluenza virus, respiratory syncytial virus.
The another kind of antigen (or antibody) that is used to catch two kinds of microorganisms to be checked of Radioactive colloidal gold that has been coated with mark on the Radioactive colloidal gold pad 14.
Referring to Fig. 2, label 21 is a lower end thieving paper, inserts during reaction in the sample, notes not making liquid level surpass the arrow of max line 22.23,24 for detecting the band position, and 25 are contrast band position.
The reaction back promptly can be observed the T district in 1 to 2 minute and the red-purple band appears in corresponding position, C district, if band appears in the C district, then the T district band occurs and represents that promptly the index of position representative is positive, and feminine gender is promptly represented in the position that band do not occur.If band does not appear in the C district, represent that then this test kit lost efficacy.
Referring to Fig. 3, band appears in the C district, and two bands appear in the T district, show to survey two indexs all positive.
Claims (2)
1, a kind of Radioactive colloidal gold of many inspections of a step detects the test kit of microorganism, it comprises base plate, Radioactive colloidal gold pad, absorption of sample pad, absorbent pad and reaction film, it is characterized in that the described Radioactive colloidal gold pad antigen that is used to catch a kind of and more than one microorganisms to be checked (or antibody) of Radioactive colloidal gold that has been coated with mark; Described reaction film is a nitrocellulose filter, be fixed with one or one or more detection band (T band) on it, with contrast band (C band), every described detection with on be embedded with the antigen (or antibody) that can catch a kind of microorganism to be checked, contrast with on be embedded with the another kind of antibody (or two anti-) of anti-this antigen (or antibody).
2, the Radioactive colloidal gold of many inspections of a step according to claim 1 detects the test kit of microorganism, it is characterized in that, microorganism that can be detected can be: one or more bacterium of intestinal bacteria, streptococcus pneumoniae, helicobacter pylori, Mycobacterium tuberculosis, candidiasis, also can be mycoplasma pneumoniae, influenza virus, parainfluenza virus, one or more viruses of respiratory syncytial virus, or comprise bacterium and virus simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02112230 CN1390949A (en) | 2002-06-25 | 2002-06-25 | Reagent kit for detecting different microbes with colloidal gold by one step |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02112230 CN1390949A (en) | 2002-06-25 | 2002-06-25 | Reagent kit for detecting different microbes with colloidal gold by one step |
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| CN1390949A true CN1390949A (en) | 2003-01-15 |
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| CN 02112230 Pending CN1390949A (en) | 2002-06-25 | 2002-06-25 | Reagent kit for detecting different microbes with colloidal gold by one step |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100399026C (en) * | 2003-12-23 | 2008-07-02 | 深圳市康美实业发展有限公司 | Quick aiagnostic reagent kid for pyloric spiral bacillus urease serum antibody and its preparing method |
| CN101699290A (en) * | 2009-04-03 | 2010-04-28 | 李克生 | Method for testing mycoplasma pneumoniae antibody, gold-labeled quick test card and preparation method |
| CN101699289A (en) * | 2009-04-03 | 2010-04-28 | 李克生 | Method for detecting toxoplasma antibody, gold-marked detection card and preparation method thereof |
| CN102411053A (en) * | 2011-08-11 | 2012-04-11 | 浙江省农业科学院 | Co-detection test strip of colibacillus 0157 and listeria monocytogenes |
| CN102928589A (en) * | 2012-11-15 | 2013-02-13 | 南京凯基生物科技发展有限公司 | Colloidal gold method detection test strip and reagent kit for IgG antibody of respiratory disease and preparation method of reagent kit |
| CN102928587A (en) * | 2012-11-16 | 2013-02-13 | 南京凯基生物科技发展有限公司 | Colloidal gold method detection test strip and reagent kit for IgM and IgG antibodies of mycoplasma pneumoniae and preparation method of reagent kit |
| CN101846677B (en) * | 2009-12-30 | 2013-06-26 | 吉林大学 | A kind of bovine tubercle bacillus antibody immune colloidal gold detection test strip and preparation method thereof |
| CN104181298A (en) * | 2014-09-02 | 2014-12-03 | 秦志浩 | Human parainfluenza virus (HPIVs) IgM antibody detection test strip and preparation method thereof |
| CN104823051A (en) * | 2012-10-05 | 2015-08-05 | 韦拉克斯生物医学股份有限公司 | Multi-analyte assay |
| CN104897892A (en) * | 2015-06-01 | 2015-09-09 | 上海凯创生物技术有限公司 | Helicobacter pylori antigen colloidal gold detection kit |
| CN105319359A (en) * | 2014-08-18 | 2016-02-10 | 董俊 | Streptococcus pneumoniae quantum dot immunochromatographic assay detection card and preparing method and application thereof |
| CN105277714B (en) * | 2014-08-18 | 2017-02-15 | 董俊 | Rapid detection method and kit for human parainfluenza virus based on magnetic separation and quantum dot labeling |
| CN107430124A (en) * | 2015-01-29 | 2017-12-01 | 滋吾堂有限公司 | The immunological detection and kit of mycoplasma pneumoniae |
| CN108977555A (en) * | 2018-03-20 | 2018-12-11 | 南京农业大学 | A kind of specific gene, detection method and the immuno-chromatographic test paper strip of fowl enteropathogenic E. Coli O78 serotype |
| CN111983229A (en) * | 2020-09-07 | 2020-11-24 | 三门县人民医院 | Reagent strip for quantitatively detecting helicobacter pylori antibody by colloidal gold and detection method |
-
2002
- 2002-06-25 CN CN 02112230 patent/CN1390949A/en active Pending
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100399026C (en) * | 2003-12-23 | 2008-07-02 | 深圳市康美实业发展有限公司 | Quick aiagnostic reagent kid for pyloric spiral bacillus urease serum antibody and its preparing method |
| CN101699290A (en) * | 2009-04-03 | 2010-04-28 | 李克生 | Method for testing mycoplasma pneumoniae antibody, gold-labeled quick test card and preparation method |
| CN101699289A (en) * | 2009-04-03 | 2010-04-28 | 李克生 | Method for detecting toxoplasma antibody, gold-marked detection card and preparation method thereof |
| CN101699290B (en) * | 2009-04-03 | 2013-12-25 | 李克生 | Method for testing mycoplasma pneumoniae antibody (IgG/IgM) gold-labeled quick test card |
| CN101846677B (en) * | 2009-12-30 | 2013-06-26 | 吉林大学 | A kind of bovine tubercle bacillus antibody immune colloidal gold detection test strip and preparation method thereof |
| CN102411053A (en) * | 2011-08-11 | 2012-04-11 | 浙江省农业科学院 | Co-detection test strip of colibacillus 0157 and listeria monocytogenes |
| CN104823051A (en) * | 2012-10-05 | 2015-08-05 | 韦拉克斯生物医学股份有限公司 | Multi-analyte assay |
| CN102928589A (en) * | 2012-11-15 | 2013-02-13 | 南京凯基生物科技发展有限公司 | Colloidal gold method detection test strip and reagent kit for IgG antibody of respiratory disease and preparation method of reagent kit |
| CN102928587A (en) * | 2012-11-16 | 2013-02-13 | 南京凯基生物科技发展有限公司 | Colloidal gold method detection test strip and reagent kit for IgM and IgG antibodies of mycoplasma pneumoniae and preparation method of reagent kit |
| CN105277714B (en) * | 2014-08-18 | 2017-02-15 | 董俊 | Rapid detection method and kit for human parainfluenza virus based on magnetic separation and quantum dot labeling |
| CN105319359A (en) * | 2014-08-18 | 2016-02-10 | 董俊 | Streptococcus pneumoniae quantum dot immunochromatographic assay detection card and preparing method and application thereof |
| CN105319359B (en) * | 2014-08-18 | 2017-02-08 | 董俊 | Streptococcus pneumoniae quantum dot immunochromatographic assay detection card and preparing method and application thereof |
| CN104181298A (en) * | 2014-09-02 | 2014-12-03 | 秦志浩 | Human parainfluenza virus (HPIVs) IgM antibody detection test strip and preparation method thereof |
| CN104181298B (en) * | 2014-09-02 | 2016-01-20 | 秦志浩 | A kind of human parainfluenza virus's IgM antibody test strip and preparation method thereof |
| CN107430124A (en) * | 2015-01-29 | 2017-12-01 | 滋吾堂有限公司 | The immunological detection and kit of mycoplasma pneumoniae |
| CN104897892A (en) * | 2015-06-01 | 2015-09-09 | 上海凯创生物技术有限公司 | Helicobacter pylori antigen colloidal gold detection kit |
| CN108977555A (en) * | 2018-03-20 | 2018-12-11 | 南京农业大学 | A kind of specific gene, detection method and the immuno-chromatographic test paper strip of fowl enteropathogenic E. Coli O78 serotype |
| CN108977555B (en) * | 2018-03-20 | 2019-07-02 | 南京农业大学 | Specific gene, detection method and immunochromatographic test strip of avian pathogenic Escherichia coli O78 serotype |
| CN111983229A (en) * | 2020-09-07 | 2020-11-24 | 三门县人民医院 | Reagent strip for quantitatively detecting helicobacter pylori antibody by colloidal gold and detection method |
| WO2022048693A1 (en) * | 2020-09-07 | 2022-03-10 | 三门县人民医院 | Reagent strip for quantitatively detecting helicobacter pylori antibody by means of colloidal gold and detection method |
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