CN1350057A - Composite ferment strain and its production process - Google Patents
Composite ferment strain and its production process Download PDFInfo
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- CN1350057A CN1350057A CN00123960A CN00123960A CN1350057A CN 1350057 A CN1350057 A CN 1350057A CN 00123960 A CN00123960 A CN 00123960A CN 00123960 A CN00123960 A CN 00123960A CN 1350057 A CN1350057 A CN 1350057A
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Abstract
The present invention discloses a microbe composition for fertilizer and feed industry and its production method. It is a ferment combined bacterial spawn at least including saccharomycetes, bacillus, cellulose-decomposing bacterium, actinomycetes, lactobacillus and aspergillus with the weight ratio of (10-30):(20-40):(10-30):(10-20):(10-15). They are respectively cultured, and mixed and adsorbed on the carrier to obtain the invented ferment combined bacterial spawn.
Description
The present invention relates to a kind of microbial composite and production method thereof that can be widely used in fields such as fertilizer, feed.Be a kind of composite ferment strain and production method thereof specifically.
The soil is the basis that the mankind depend on for existence, and for generations, all the time, people sow in spring and reap in autumn every year.Human inexhaustible wealth is given in the soil.Yet for a long time, people use chemical fertilizer in a large number, have brought serious negative interaction to the soil.Original microecological balance is broken in the soil, and soil hardening hardens, and protects fertile water-retentivity and reduces greatly, and disease and pest constantly aggravates, and the synthetic pesticide consumption increases severely, and crop quality and output constantly descend.More prior is that the hazard residue thing of fertilizer and pesticide produces adverse consequences to human body in the vegetables that we ate, the fruit.Enzymatic microorganism farming method is this microbial technique that the forties grows up in this century of Japanese microbiologist island, is an agricultural high-tech patent of Japan, is widely used in fields such as plant husbandry, aquaculture.It not only can increase considerably agricultural output, can also improve quality, reaches high yield and high quality, non-harmful purpose.Can also significantly reduce simultaneously pollution to physical environment: the Shanghai Lexicographic Publishing House publish 1989 version " Ci hai " to the explanation of " ferment " speech be: being once called as of " enzyme "; And to the explanation of " enzyme " be: the protein that organism produces with catalytic capability.As seen, the meaning of enzymatic microorganism is exactly: the microorganism that can produce enzyme.Enzymatic microorganism farming method just is meant the application of microorganism on agricultural.Microorganism and composition thereof can also be applied to aquaculture, mainly are to make various microorganism feed addictive.This additive adds the raising that can be used for various livestock and poultry and special animals in the feed by a certain percentage.For example application number is that 96109854.6 Chinese patent application discloses a kind of " efficient biological compound fertilizer and preparation method thereof ", said composite fertilizer is the fermented liquid mixed solution that several genus bacillus and phosphate solubilizing bacteria, rhizosphere combination azotobacter are fermented respectively in this patent application, with the various plants trace element and sorbent material is mixed must.Again for example: number of patent application is that 98109527 Chinese patent application discloses a kind of " microecologic freshwater fish feed ", it is to be work in-process by three kinds of micro-ecological feeds, the ratio of determining with orthogonal experiment adds the full price fish meal and forms product, and these three kinds of micro-ecological feed raw materials are: is the active animal protein powder that the fermented mixture of base-material forms with probiotic bacteria to the animal proteinum raw material; The photosynthetic probiotics yeast that the vegetable protein fermenting raw materials is formed with photosynthetic bacterium, bacillus cereus and some yeast; And the soil fodder that forms with the fermenting raw materials such as pure pollution-free soil of candida tropicalis and some probiotic bacteria.
The above-mentioned various microbial composite kind that is used for fertilizer and feedstuff industry is single, effect is also more single, and the cultivation of microorganism is more complicated also, and price is higher.
The purpose of this invention is to provide a kind of plant husbandry and aquaculture as fertilizer sources and feed, simple, the low-cost microbial composite of method for culturing microbes and production method thereof of being widely used in.
For achieving the above object, the present invention adopts following technical scheme:
Composite ferment strain of the present invention comprises yeast, genus bacillus, cellulose-decomposing bacterium, actinomycetes, Bacterium lacticum and aspergillus at least, and wherein yeast, genus bacillus, cellulose-decomposing bacterium, actinomycetes, Bacterium lacticum and aspergillar quantity are than being (10-30): (20-40): (10-30): (10-20): (10-15).
As the further improvement to composite ferment strain of the present invention, described yeast is the mixture that comprises the arbitrary proportion of saccharomyces hansenii, cereuisiae fermentum; Described genus bacillus is the mixture that comprises the arbitrary proportion of subtilis, Bacillus licheniformis; Described cellulose-decomposing bacterium is that the Gram-negative Mierocrystalline cellulose decomposes dialister bacterium; Described actinomycetes are actinomyces israeliis; Described Bacterium lacticum is a lactobacillus fermentum; Described aspergillus is aspergillus niger.
The production method of composite ferment strain of the present invention comprises the steps: at least
One, preparation substratum
(1), potato glucose substratum
With peeling potatoes, be cut into piece, add water boil 15-45 minute, use filtered through gauze then, the potato after filtering being mixed according to weight ratio with glucose and water at 10: 1: 50 and place it in pressure is that 0.8-1.5 kilogram/square centimeter, temperature are to sterilize 10-30 minute in 110-150 degree centigrade the environment.
(2), nutrient broth medium
With extractum carnis, peptone, sodium-chlor and water according to weight ratio 3: 10: 5: 1000 mix, and accents pH value is 7.0-7.2, and to place it in pressure be that 0.8-1.5 kilogram/square centimeter, temperature are to sterilize 10-30 minute in 110-150 degree centigrade the environment.
(3), inorganic salt basic medium
(ammonium nitrate: three water dipotassium hydrogen phosphates: potassium primary phosphate: magnesium sulfate heptahydrate: sodium-chlor: calcium chloride: iron trichloride: yeast extract paste: water) be 1.0: 0.5: 0.5: 0.5: 1.0: 1.0: it is 7.0-7.2 that 0.02: 0.05: 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to the inorganic salt basic medium that aforementioned proportion prepares and be 0.8-1.5 kilogram/square centimeter, temperature and be in 110-150 degree centigrade the environment sterilization 10-30 minute.
(4), No. 1 substratum of Gao Shi
(Zulkovsky starch: saltpetre: sodium-chlor: dipotassium hydrogen phosphate: sal epsom: iron vitriol: water) be 20: 1: 0.5: 0.5: 0.5: 0.01: it is 7.2-7.4 that 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to No. 1 substratum of Gao Shi that aforementioned proportion prepares and be 0.8-1.5 kilogram/square centimeter, temperature and be in 110-150 degree centigrade the environment sterilization 10-30 minute.
(5), PYG substratum
(peptone: yeast extract paste: glucose: water) be 10: 5: 20: it is 6.5-7.0 that 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to the PYG substratum that aforementioned proportion prepares and be 0.8-1.5 kilogram/square centimeter, temperature and be in 110-150 degree centigrade the environment sterilization 10-30 minute.
(6), czapek's solution
According to weight ratio (SODIUMNITRATE: dipotassium hydrogen phosphate: Repone K: sal epsom: ferrous sulfate: sucrose: water) be 2: 1: 0.5: 0.5: 0.01: 30: placed it in pressure after 1000 ratio prepares and be 0.8-1.5 kilogram/square centimeter, temperature and be in 110-150 degree centigrade the environment sterilization 10-30 minute.
Two, cultivate bacterial strain
Be seeded in yeast on the above-mentioned potato glucose substratum and place 33-35 degree centigrade incubator to cultivate 3-5 days.
Be seeded in genus bacillus on the above-mentioned nutrient broth medium and place 33-35 degree centigrade incubator to cultivate 3-5 days.
Be seeded in cellulose-decomposing bacterium on the above-mentioned inorganic salt basic medium and place 40-60 degree centigrade incubator to cultivate 5-10 days.
Be seeded in actinomycetes on No. 1 substratum of above-mentioned Gao Shi and place 33-35 degree centigrade incubator to cultivate 3-5 days.
Be seeded in Bacterium lacticum on the above-mentioned PYG substratum and place 33-35 degree centigrade incubator fermentation 8-12 hour.
Be seeded in aspergillus on the above-mentioned czapek's solution and place 33-35 degree centigrade incubator to cultivate 3-5 days.
Three, be mixed and made into product
With above-mentioned cultured each bacterial classification together with nutrient solution according to yeast: genus bacillus: cellulose-decomposing bacterium: actinomycetes: Bacterium lacticum: aspergillar quantity is than being (10-30): (20-40): (10-30): (10-20): ratio (10-15) is adsorbed on the solid carrier with alr mode, dries then or dries in temperature is not higher than 70 degrees centigrade drying machine.Described solid carrier can be one or more 20-50 purpose granular carriers that are ground into of sawdust, rice chaff, wheat bran, plant straw.
After adopting technique scheme, following positively effect can be arranged:
1, yeast: aerobic conditions produces nutritious single-cell protein down, produces nutritive substance and various VITAMIN (based on B family) promotion animal and plant growth that small molecules such as various alcohols, organic acid, aromatic compound are easily received at the oxygen free condition bottom fermentation.2, genus bacillus: having a large amount of amylase, proteolytic enzyme, rnase (DNA enzyme), lecithinase, can transforming the macromolecular substance that is difficult for absorbing is the small molecules nutrition that is easy to absorb, and promotes the breeding of multiple beneficial microbial growth.Potassium in the conversion silicate is for can utilize attitude potassium.The breeding of aerobic spore-bearing bacilli in enteron aisle can absorb the remaining oxygen in the enteron aisle, forms oxygen-free environment, helps the growth and breeding of probioticss such as bifidus bacillus.3, cellulose-decomposing bacterium: have the powerful crop straw that decomposes, be converted into the nutraceutical effect of organic molecule.4, actinomycetes: can produce microbiotic, suppress the growth of harmful bacterium, synthesis capability is strong.5, Bacterium lacticum: acid of producing and the effect that suppresses enteric pathogenic bacteria are arranged, also can synthesise vitamins.6, aspergillus: contain amylase, proteolytic enzyme and cellulase, transforming provides the small molecules nutritive substance, promotes the beneficial microorganism growth.
Through the multiple spot evidence, this microbial composite is except that having effect brilliance, efficient, strong stress resistance, be that composite fungus agent also has following characteristics outward: 1, have extremely strong decomposition, fermentation conversion capability, organic substance and inorganic mineral can be changed into effective nutrition, improve the utilization ratio of fertilizer and feed greatly; Hot properties when 2, relying on its enlarged culturing and the antagonistic action between microorganism can play the effect of killing to harmful microorganism, effectively reduce the generation of disease and pest; 3, this bacterial classification can suppress enteropahtogenic microganism, clears up the objectionable impurities in the body, thereby eliminates virulence factor, improves microecological balance, promotes the digestion and absorption function of human body and animal; 4, this bacterial classification can be secreted the large number of biological organized enzyme, the material that is difficult for passive plant utilization is converted into can utilizes attitude, sets up and keep microecological balance, and synthetic auxin, promotes that animal and plant grow, and improves crop yield and quality.5, the culture of strains method simple, can reduce production costs, easy to utilize.
Further specify the present invention below in conjunction with specific embodiment:
Embodiment one:
One, preparation substratum
(1), potato glucose substratum
With peeling potatoes, be cut into piece, added water boil 30 minutes, and used filtered through gauze then, the potato after filtering being mixed according to weight ratio with glucose and water at 10: 1: 50 and place it in pressure is that 1.05 kilograms/square centimeter, temperature are to sterilize 20 minutes in 121.3 degrees centigrade the environment.
(2), nutrient broth medium
With extractum carnis, peptone, sodium-chlor and water according to weight ratio 3: 10: 5: 1000 mix, and transferring pH value is 7.1, and to place it in pressure be that 1.05 kilograms/square centimeter, temperature are sterilization 20 minutes in 121.3 degrees centigrade the environment.
(3), inorganic salt basic medium
(ammonium nitrate: three water dipotassium hydrogen phosphates: potassium primary phosphate: magnesium sulfate heptahydrate: sodium-chlor: calcium chloride: iron trichloride: yeast extract paste: water) be 1.0: 0.5: 0.5: 0.5: 1.0: 1.0: it is 7.1 that 0.02: 0.05: 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to the inorganic salt basic medium that aforementioned proportion prepares and be 1.05 kilograms/square centimeter, temperature and be in 121.3 degrees centigrade the environment sterilization 20 minutes.
(4), No. 1 substratum of Gao Shi
(Zulkovsky starch: saltpetre: sodium-chlor: dipotassium hydrogen phosphate: sal epsom: iron vitriol: water) be 20: 1: 0.5: 0.5: 0.5: 0.01: it is 7.3 that 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to No. 1 substratum of Gao Shi that aforementioned proportion prepares and be 1.05 kilograms/square centimeter, temperature and be in 121.3 degrees centigrade the environment sterilization 20 minutes.
(5), PYG substratum
(peptone: yeast extract paste: glucose: water) be 10: 5: 20: it is 6.8 that 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to the PYG substratum that aforementioned proportion prepares and be 1.05 kilograms/square centimeter, temperature and be in 121.3 degrees centigrade the environment sterilization 20 minutes.
(6), czapek's solution
According to weight ratio (SODIUMNITRATE: dipotassium hydrogen phosphate: Repone K: sal epsom: ferrous sulfate: sucrose: water) be 2: 1: 0.5: 0.5: 0.01: 30: placed it in pressure after 1000 ratio prepares and be 1.05 kilograms/square centimeter, temperature and be in 121.3 degrees centigrade the environment sterilization 20 minutes.
Two, cultivate bacterial strain
Be seeded in yeast on the above-mentioned potato glucose substratum and place 33-35 degree centigrade incubator to cultivate 3 days.
Be seeded in genus bacillus on the above-mentioned nutrient broth medium and place 33-35 degree centigrade incubator to cultivate 3 days.
Be seeded in cellulose-decomposing bacterium on the above-mentioned inorganic salt basic medium and place 40-60 degree centigrade incubator to cultivate 7 days.
Be seeded in actinomycetes on No. 1 substratum of above-mentioned Gao Shi and place 33-35 degree centigrade incubator to cultivate 3 days.
Be seeded in Bacterium lacticum on the above-mentioned PYG substratum and place 33-35 degree centigrade incubator fermentation 8 hours.
Be seeded in aspergillus on the above-mentioned czapek's solution and place 33-35 degree centigrade incubator to cultivate 3 days.
The concentration of adjusting used sodium hydroxide of above-mentioned substratum and hydrochloric acid is 1mol/l.
Three, be mixed and made into product
With above-mentioned cultured each bacterial classification together with nutrient solution according to being adsorbed on the solid carrier with alr mode according to one of ratio shown in the table 1 (quantity than), dry then or in temperature is not higher than 70 degrees centigrade drying machine, dry.Described solid carrier can be one or more 20-50 purpose granular carriers that are ground into of sawdust, rice chaff, wheat bran, plant straw.
Embodiment two:
One, preparation substratum
(1), potato glucose substratum
With peeling potatoes, be cut into piece, added water boil 30 minutes, and used filtered through gauze then, the potato after filtering being mixed according to weight ratio with glucose and water at 10: 1: 50 and place it in pressure is that 0.8 kilogram/square centimeter, temperature are to sterilize 40 minutes in 110 degrees centigrade the environment.
(2), nutrient broth medium
With extractum carnis, peptone, sodium-chlor and water according to weight ratio 3: 10: 5: 1000 mix, and transferring pH value is 7.1, and to place it in pressure be that 0.8 kilogram/square centimeter, temperature are sterilization 40 minutes in 110 degrees centigrade the environment.
(3), inorganic salt basic medium
(ammonium nitrate: three water dipotassium hydrogen phosphates: potassium primary phosphate: magnesium sulfate heptahydrate: sodium-chlor: calcium chloride: iron trichloride: yeast extract paste: water) be 1.0: 0.5: 0.5: 0.5: 1.0: 1.0: it is 7.1 that 0.02: 0.05: 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to the inorganic salt basic medium that aforementioned proportion prepares and be 0.8 kilogram/square centimeter, temperature and be in 110 degrees centigrade the environment sterilization 40 minutes.
(4), No. 1 substratum of Gao Shi
(Zulkovsky starch: saltpetre: sodium-chlor: dipotassium hydrogen phosphate: sal epsom: iron vitriol: water) be 20: 1: 0.5: 0.5: 0.5: 0.01: it is 7.3 that 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to No. 1 substratum of Gao Shi that aforementioned proportion prepares and be 0.8 kilogram/square centimeter, temperature and be in 110 degrees centigrade the environment sterilization 40 minutes.
(5), PYG substratum
(peptone: yeast extract paste: glucose: water) be 10: 5: 20: it is 6.8 that 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to the PYG substratum that aforementioned proportion prepares and be 0.8 kilogram/square centimeter, temperature and be in 110 degrees centigrade the environment sterilization 40 minutes.
(6), czapek's solution
According to weight ratio (SODIUMNITRATE: dipotassium hydrogen phosphate: Repone K: sal epsom: ferrous sulfate: sucrose: water) be 2: 1: 0.5: 0.5: 0.01: 30: placed it in pressure after 1000 ratio prepares and be 0.8 kilogram/square centimeter, temperature and be in 110 degrees centigrade the environment sterilization 40 minutes.
Two, cultivate bacterial strain
Be seeded in yeast on the above-mentioned potato glucose substratum and place 33-35 degree centigrade incubator to cultivate 4 days.
Be seeded in genus bacillus on the above-mentioned nutrient broth medium and place 33-35 degree centigrade incubator to cultivate 4 days.
Be seeded in cellulose-decomposing bacterium on the above-mentioned inorganic salt basic medium and place 40-60 degree centigrade incubator to cultivate 8 days.
Be seeded in actinomycetes on No. 1 substratum of above-mentioned Gao Shi and place 33-35 degree centigrade incubator to cultivate 4 days.
Be seeded in Bacterium lacticum on the above-mentioned PYG substratum and place 33-35 degree centigrade incubator fermentation 10 hours.
Be seeded in aspergillus on the above-mentioned czapek's solution and place 33-35 degree centigrade incubator to cultivate 4 days.
The concentration of adjusting used sodium hydroxide of above-mentioned substratum and hydrochloric acid is 1mol/l.
Three, be mixed and made into product
With above-mentioned cultured each bacterial classification together with nutrient solution according to being adsorbed on the solid carrier with alr mode according to one of ratio shown in the table 1 (quantity than), dry then or in temperature is not higher than 70 degrees centigrade drying machine, dry.Described solid carrier can be one or more 20-50 purpose granular carriers that are ground into of sawdust, rice chaff, wheat bran, plant straw.
Embodiment three:
One, preparation substratum
(1), potato glucose substratum
With peeling potatoes, be cut into piece, added water boil 30 minutes, and used filtered through gauze then, the potato after filtering being mixed according to weight ratio with glucose and water at 10: 1: 50 and place it in pressure is that 1.5 kilograms/square centimeter, temperature are to sterilize 20 minutes in 150 degrees centigrade the environment.
(2), nutrient broth medium
With extractum carnis, peptone, sodium-chlor and water according to weight ratio 3: 10: 5: 1000 mix, and transferring pH value is 7.1, and to place it in pressure be that 1.5 kilograms/square centimeter, temperature are sterilization 20 minutes in 150 degrees centigrade the environment.
(3), inorganic salt basic medium
(ammonium nitrate: three water dipotassium hydrogen phosphates: potassium primary phosphate: magnesium sulfate heptahydrate: sodium-chlor: calcium chloride: iron trichloride: yeast extract paste: water) be 1.0: 0.5: 0.5: 0.5: 1.0: 1.0: it is 7.1 that 0.02: 0.05: 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to the inorganic salt basic medium that aforementioned proportion prepares and be 1.5 kilograms/square centimeter, temperature and be in 150 degrees centigrade the environment sterilization 20 minutes.
(4), No. 1 substratum of Gao Shi
(Zulkovsky starch: saltpetre: sodium-chlor: dipotassium hydrogen phosphate: sal epsom: iron vitriol: water) be 20: 1: 0.5: 0.5: 0.5: 0.01: it is 7.3 that 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to No. 1 substratum of Gao Shi that aforementioned proportion prepares and be 1.5 kilograms/square centimeter, temperature and be in 150 degrees centigrade the environment sterilization 20 minutes.
(5), PYG substratum
(peptone: yeast extract paste: glucose: water) be 10: 5: 20: it is 6.8 that 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to the PYG substratum that aforementioned proportion prepares and be 1.5 kilograms/square centimeter, temperature and be in 150 degrees centigrade the environment sterilization 20 minutes.
(6), czapek's solution
According to weight ratio (SODIUMNITRATE: dipotassium hydrogen phosphate: Repone K: sal epsom: ferrous sulfate: sucrose: water) be 2: 1: 0.5: 0.5: 0.01: 30: placed it in pressure after 1000 ratio prepares and be 1.5 kilograms/square centimeter, temperature and be in 150 degrees centigrade the environment sterilization 20 minutes.
Two, cultivate bacterial strain
Be seeded in yeast on the above-mentioned potato glucose substratum and place 33-35 degree centigrade incubator to cultivate 5 days.
Be seeded in genus bacillus on the above-mentioned nutrient broth medium and place 33-35 degree centigrade incubator to cultivate 5 days.
Be seeded in cellulose-decomposing bacterium on the above-mentioned inorganic salt basic medium and place 40-60 degree centigrade incubator to cultivate 8 days.
Be seeded in actinomycetes on No. 1 substratum of above-mentioned Gao Shi and place 33-35 degree centigrade incubator to cultivate 5 days.
Be seeded in Bacterium lacticum on the above-mentioned PYG substratum and place 33-35 degree centigrade incubator fermentation 12 hours.
Be seeded in aspergillus on the above-mentioned czapek's solution and place 33-35 degree centigrade incubator to cultivate 5 days.
The concentration of adjusting used sodium hydroxide of above-mentioned substratum and hydrochloric acid is 1mol/l.
Three, be mixed and made into product
With above-mentioned cultured each bacterial classification together with nutrient solution according to being adsorbed on the solid carrier with alr mode according to one of ratio shown in the table 1 (quantity than), dry then or in temperature is not higher than 70 degrees centigrade drying machine, dry.Described solid carrier can be one or more 20-50 purpose granular carriers that are ground into of sawdust, rice chaff, wheat bran, plant straw.
The influence of when table 2 demonstrates composite ferment strain of the present invention and is used for vegetables fertilizer vegetable seedling being grown.
Table 1
| Yeast | Genus bacillus | Cellulose-decomposing bacterium | Actinomycetes | Bacterium lacticum | Aspergillus | |||
| The saccharomyces hansenii bacterium | Saccharomyces cerevisiae | Bacillus licheniformis | Subtilis | |||||
| Example 1 | ?10 | ?10 | ?15 | ?15 | ?15 | ?10 | ?10 | ?15 |
| Example 2 | ?7.5 | ?7.5 | ?20 | ?20 | ?15 | ?10 | ?10 | ?10 |
| Example 3 | ?15 | ?15 | ?10 | ?10 | ?10 | ?15 | ?15 | ?10 |
| Example 4 | ?5 | ?5 | ?15 | ?15 | ?15 | ?10 | ?20 | ?15 |
| Example 5 | ?20 | ?0 | ?30 | ?0 | ?15 | ?10 | ?10 | ?15 |
| Example 6 | ?0 | ?20 | ?0 | ?30 | ?15 | ?10 | ?10 | ?15 |
| Example 7 | ?5 | ?5 | ?15 | ?15 | ?15 | ?20 | ?10 | ?12 |
| Example 8 | ?30 | ?0 | ?40 | ?0 | ?10 | ?20 | ?10 | ?15 |
| Example 9 | ?0 | ?30 | ?0 | ?40 | ?12 | ?18 | ?16 | ?13 |
Table 2
| Crop | Fertilizer | Plant height (cm) | Stem thick (cm) | Cauline leaf dry weight (g) | Root dry weight (g) | Chlorophyll content (mg/g fw) | Strong sprout index |
| Cucumber | Example 1 | ??75.3 | ??7.36 | ????8.15 | ??1.31 | ????4.876 | |
| Chemical fertilizer | ??70.6 | ??6.99 | ????7.41 | ??1.04 | ????4.342 | ||
| Capsicum | Example 1 | ??24.6 | ??6.49 | ????5.089 | ??0.0177 | ||
| Chemical fertilizer | ??19.6 | ??6.31 | ????4.352 | ??0.0103 |
Claims (3)
1, a kind of composite ferment strain, it is characterized in that: described composite ferment strain comprises yeast, genus bacillus, cellulose-decomposing bacterium, actinomycetes, Bacterium lacticum and aspergillus at least, and wherein yeast, genus bacillus, cellulose-decomposing bacterium, actinomycetes, Bacterium lacticum and aspergillar quantity are than being (10-30): (20-40): (10-30): (10-20): (10-15).
2, composite ferment strain according to claim 1 is characterized in that: described yeast is the mixture that comprises the arbitrary proportion of saccharomyces hansenii, cereuisiae fermentum; Described genus bacillus is the mixture that comprises the arbitrary proportion of subtilis, Bacillus licheniformis; Described cellulose-decomposing bacterium is that the Gram-negative Mierocrystalline cellulose decomposes dialister bacterium; Described actinomycetes are actinomyces israeliis; Described Bacterium lacticum is a lactobacillus fermentum; Described aspergillus is aspergillus niger.
3, a kind of production method of composite ferment strain is characterized in that: this method comprises the steps: at least
One, preparation substratum
(1), potato glucose substratum
With peeling potatoes, be cut into piece, add water boil 15-45 minute, use filtered through gauze then, the potato after filtering being mixed according to weight ratio with glucose and water at 10: 1: 50 and place it in pressure is that 0.8-1.5 kilogram/square centimeter, temperature are to sterilize 10-30 minute in 110-150 degree centigrade the environment.
(2), nutrient broth medium
With extractum carnis, peptone, sodium-chlor and water according to weight ratio 3: 10: 5: 1000 mix, and accents pH value is 7.0-7.2, and to place it in pressure be that 0.8-1.5 kilogram/square centimeter, temperature are to sterilize 10-30 minute in 110-150 degree centigrade the environment.
(3), inorganic salt basic medium
(ammonium nitrate: three water dipotassium hydrogen phosphates: potassium primary phosphate: magnesium sulfate heptahydrate: sodium-chlor: calcium chloride: iron trichloride: yeast extract paste: water) be 1.0: 0.5: 0.5: 0.5: 1.0: 1.0: it is 7.0-7.2 that 0.02: 0.05: 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to the inorganic salt basic medium that aforementioned proportion prepares and be 0.8-1.5 kilogram/square centimeter, temperature and be in 110-150 degree centigrade the environment sterilization 10-30 minute.
(4), No. 1 substratum of Gao Shi
(Zulkovsky starch: saltpetre: sodium-chlor: dipotassium hydrogen phosphate: sal epsom: iron vitriol: water) be 20: 1: 0.5: 0.5: 0.5: 0.01: it is 7.2-7.4 that 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to No. 1 substratum of Gao Shi that aforementioned proportion prepares and be 0.8-1.5 kilogram/square centimeter, temperature and be in 110-150 degree centigrade the environment sterilization 10-30 minute.
(5), PYG substratum
(peptone: yeast extract paste: glucose: water) be 10: 5: 20: it is 6.5-7.0 that 1000 ratio prepares and transfer pH value according to weight ratio.To be placed on pressure according to the PYG substratum that aforementioned proportion prepares and be 0.8-1.5 kilogram/square centimeter, temperature and be in 110-150 degree centigrade the environment sterilization 10-30 minute.
(6), czapek's solution
According to weight ratio (SODIUMNITRATE: dipotassium hydrogen phosphate: Repone K: sal epsom: ferrous sulfate: sucrose: water) be 2: 1: 0.5: 0.5: 0.01: 30: placed it in pressure after 1000 ratio prepares and be 0.8-1.5 kilogram/square centimeter, temperature and be in 110-150 degree centigrade the environment sterilization 10-30 minute.
Two, cultivate bacterial strain
Be seeded in yeast on the above-mentioned potato glucose substratum and place 33-35 degree centigrade incubator to cultivate 3-5 days.
Be seeded in genus bacillus on the above-mentioned nutrient broth medium and place 33-35 degree centigrade incubator to cultivate 3-5 days.
Be seeded in cellulose-decomposing bacterium on the above-mentioned inorganic salt basic medium and place 40-60 degree centigrade incubator to cultivate 5-10 days.
Be seeded in actinomycetes on No. 1 substratum of above-mentioned Gao Shi and place 33-35 degree centigrade incubator to cultivate 3-5 days.
Be seeded in Bacterium lacticum on the above-mentioned PYG substratum and place 33-35 degree centigrade incubator fermentation 8-12 hour.
Be seeded in aspergillus on the above-mentioned czapek's solution and place 33-35 degree centigrade incubator to cultivate 3-5 days.
Three, be mixed and made into product
With above-mentioned cultured each bacterial classification together with substratum according to yeast: genus bacillus: cellulose-decomposing bacterium: actinomycetes: Bacterium lacticum: aspergillar quantity is than being (10-30): (20-40): (10-30): (10-20): ratio (10-15) is adsorbed on the solid carrier with alr mode, dries then or dries in temperature is not higher than 70 degrees centigrade drying machine.Described solid carrier can be one or more 20-50 purpose granular carriers that are ground into of sawdust, rice chaff, wheat bran, plant straw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00123960A CN1350057A (en) | 2000-10-25 | 2000-10-25 | Composite ferment strain and its production process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00123960A CN1350057A (en) | 2000-10-25 | 2000-10-25 | Composite ferment strain and its production process |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1881948A2 (en) * | 2005-02-22 | 2008-01-30 | EVL Inc. | Enhanced fertilizer and method for producing same |
| CN101368165B (en) * | 2008-08-12 | 2011-01-12 | 辽宁医学院 | Probiotic composition for livestock and poultry |
| CN103385362A (en) * | 2013-04-18 | 2013-11-13 | 湖北宏全生物科技有限公司 | Preparation method for biological microbial protease preparation |
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2000
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1881948A2 (en) * | 2005-02-22 | 2008-01-30 | EVL Inc. | Enhanced fertilizer and method for producing same |
| EP1881948A4 (en) * | 2005-02-22 | 2011-11-23 | Evl Inc | Enhanced fertilizer and method for producing same |
| CN101368165B (en) * | 2008-08-12 | 2011-01-12 | 辽宁医学院 | Probiotic composition for livestock and poultry |
| CN103385362A (en) * | 2013-04-18 | 2013-11-13 | 湖北宏全生物科技有限公司 | Preparation method for biological microbial protease preparation |
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