CN1290859A - Method and device for on-line monitoring waste water - Google Patents
Method and device for on-line monitoring waste water Download PDFInfo
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- CN1290859A CN1290859A CN 00116096 CN00116096A CN1290859A CN 1290859 A CN1290859 A CN 1290859A CN 00116096 CN00116096 CN 00116096 CN 00116096 A CN00116096 A CN 00116096A CN 1290859 A CN1290859 A CN 1290859A
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- 239000002351 wastewater Substances 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000012544 monitoring process Methods 0.000 title claims description 14
- 238000005259 measurement Methods 0.000 claims abstract description 6
- 239000002699 waste material Substances 0.000 claims abstract description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 20
- 229910052760 oxygen Inorganic materials 0.000 claims description 20
- 239000001301 oxygen Substances 0.000 claims description 20
- 230000004044 response Effects 0.000 claims description 15
- 230000002572 peristaltic effect Effects 0.000 claims description 7
- 230000000813 microbial effect Effects 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 239000002028 Biomass Substances 0.000 claims description 2
- 238000005070 sampling Methods 0.000 claims 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 20
- 230000008859 change Effects 0.000 abstract description 8
- 238000001514 detection method Methods 0.000 abstract description 2
- 230000002906 microbiologic effect Effects 0.000 description 14
- 244000005700 microbiome Species 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- 238000005188 flotation Methods 0.000 description 4
- 238000004065 wastewater treatment Methods 0.000 description 4
- WFJIVOKAWHGMBH-UHFFFAOYSA-N 4-hexylbenzene-1,3-diol Chemical compound CCCCCCC1=CC=C(O)C=C1O WFJIVOKAWHGMBH-UHFFFAOYSA-N 0.000 description 3
- 238000013016 damping Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000003760 magnetic stirring Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000004401 flow injection analysis Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000036284 oxygen consumption Effects 0.000 description 2
- 240000008886 Ceratonia siliqua Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
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- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
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- 238000001303 quality assessment method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
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- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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Abstract
Microbe screened from waste water is utilized to constitute waste BOD measuring microbe sensor, which, after calibrated with standard GGA solution, is used in quantitative measurement of BOD of waste water flowing through constantly. By means of the method and device of the present invention, BOD curve of waste water may be measured and the property change of waste water may be obtained. The present invention is suitable for fast detection and estimation of water quality.
Description
The present invention relates to the method for on-line monitoring waste water BOD and the device of realizing this method, method and apparatus of the present invention is particularly suitable for measuring the BOD value of petrochemical wastewater.
BOD (Biochemical Oxygen Demand), promptly biochemical oxygen demand (BOD) is a water body organic contamination overall target, has significant values at aspects such as water quality assessment, wastewater biodegradability assessments, its notion and method have been continued to use last 100 years.Yet classical BOD determination method (being BOD5 standard dilution method) adopts microorganism cultivation on the 5th, complex operation, consuming time, labor capacity big, just can obtain measurement result later in 5 days, be difficult to be directly used in and instruct biological wastewater treatment, be a very big shortcoming.
Japan scholar Karube has invented BOD microbiological sensor method; can record waste water BOD value at tens of minutes, and repeatability better, is a kind of challenging methodology innovation; be subjected to the favor of scholar and environmental protection department, the continuity of relevant research report constantly.The principle of BOD microbiological sensor also is based on microorganism to organic oxygen consumption metabolism, be with the difference of classic method, classic method is calculated the BOD value by the absolute oxygen utilization of cultivating 5 days, and microbiological sensor method mensuration BOD only relates to initial oxidation speed.Immobilized microorganism and oxygen electrode are constituted sensor, containing in the water body can be biochemical during organism, and microbial film can utilize organic substrates to carry out external source and breathe, thereby consumes the dissolved oxygen DO in the water body, the output current of oxygen electrode is descended, and the current value of decline and BOD concentration have certain linear.About 10 minutes of the response time of this method, the mensuration cycle was compared with classic method about 30 minutes, and is not only quick, and easy and simple to handle, is easy to robotization.If this microbiological sensor method can directly link to each other with wastewater treatment equipment, online (on line) measures BOD, can more in time carry out the monitoring of wastewater treatment, improves waste water treatment efficiency.
The method that the purpose of this invention is to provide a kind of on-line monitoring waste water BOD is utilized microbiological sensor on-line monitoring petrochemical wastewater BOD value, and method is simple for this, and response is stable, can in time reflect the water quality characteristic of tested waste water.
Another object of the present invention provides the device of this waste water on-line monitoring.
For achieving the above object, the present invention adopts following technical measures: the BOD microbiological sensor that utilizes the applicant to make up is formed flow type and is measured system; The parameter of staking-out work system is carried out on-line determination.The steps include:
A. adopt the microbiological sensor (ACTA Scientiae Circumstantiae of having reported, 1986,6:184-192), biomass, sandwich method with 0.750D/ml are made the microorganism mycoderm, the method that the acclimated microorganism mycoderm adopts the petrochemical wastewater of the standard GGA solution of 33mg/L, about 30mg/L successively respectively to tame 12 hours.
B. with flow type BOD determinator (see figure 1), carry out Performance Detection such as responding range, mensuration cycle, the coefficient of variation, correlativity.
C. flow through after work system makes it to reach steady state (SS) with blank phosphate buffer, with the standard GGA standardization of solution work system of 16.5mg/L, output signal is recorded on the recording chart through oxygen analyser, or prints through main frame.Again with various types of waste water 48 hours incessantly the continuous flow injection analyze, directly reflected by the situation of change of response current the change of water quality of waste water to be on-line determination.In the on-line monitoring experiment, BOD value and the classic method that sensor is measured very close (seeing Table 1), the situation of change of response current has directly reflected the character (see figure 2) of different waste water.
Be used to realize that the device of method of the present invention comprises:
A waste water treater has waste water injection port, waste water outlet and a magnetic stirring apparatus;
A peristaltic pump, import connects waste water outlet and blank damping fluid hold-up vessel, and outlet connects microbiological sensor;
A microbiological sensor is made up of biosensor and oxygen electrode, has freeing port and connects waste liquid pool, and oxygen electrode is communicated with the measuring instrument main frame.
The present invention compares with the technology of existing monitoring waste water, have the following advantages and effect: simplified the origin operation step, shortened the cycle of waste water BOD pH-value determination pH greatly, measure easily and fast, respond reliable and stable, substantially realize the on-line monitoring of waste water, can reflect the water quality characteristic of tested waste water, be applicable to the fast detecting and the assessment of water quality.
Fig. 1 is a kind of flow type BOD assay device structures synoptic diagram.
Fig. 2 is on-line monitoring BOD value change curve synoptic diagram.
Below in conjunction with accompanying drawing the present invention is explained in further detail.
According to Fig. 1 as can be known, waste water treater 1 has a waste water injection port 2 and a waste water outlet 3, underlying magnetic stirring apparatus 8, and waste water outlet 3 links to each other with peristaltic pump 4; Peristaltic pump 4 is communicated with blank damping fluid hold-up vessel 10 and microbiological sensor 5; Microbiological sensor 5 is made up of biosensor 6 and oxygen electrode 7, and oxygen electrode 7 is communicated with main frame 11, and sensor 5 has a freeing port and is communicated with waste liquid pool 9.
This determinator is made up of microbiological sensor, peristaltic pump and main frame three parts.Main frame comprises stabilized voltage supply electric power system, mimic channel amplification system, I/F converting system, single-chip computer control system and software thereof, LD digital display unit, parts such as mini-printer and shell.This device has following basic function: the mensuration mode is selected: dynamic method and steady state method; Self-poise prompting, the prompting application of sample is printed automatically and is shown measurement result, sample number and BOD concentration value.
The method of on-line determination is: waste water to be measured at first enters waste water treater 1 through water inlet 2, carries out saturated dissolved oxygen process under the stirring of magnetic stirring apparatus 8; Secondly the waste water behind the oxygen saturation is mixed into peristaltic pump 4 through outlet 3 and a certain amount of blank damping fluid 10, comes the control system flow velocity to be about 1mL/min by peristaltic pump; The waste water sample that flows out enters microbiological sensor 5, biosensor 6 wherein (microorganism mycoderm) but carry out the oxygen consumption metabolism for the biochemical substances in the waste water, consume the oxygen in the solution, the oxygen electrode that closely contacts with biological membrane 7 responds to this, the reduction of oxygen is converted into the variation of current value, be conveyed into main frame 11, waste liquid drains into waste liquid pool 9.The variation of response current is shown by main frame 11 and prints.
According to Fig. 2 as can be known, in the on-line monitoring experiment, we utilize the mode of oxygen analyser linkage record instrument, have drawn the waste water of the flowing through influence curve figure to response current.As seen, the A stage is that the standard GGA flow of solution of 16.5mg/L is through BOD microbiological sensor 1 hour, calibration system parameter among the figure; The B stage is that the water outlet of 1# flotation cell was flowed through about 9 hours, and the response variation in 2.4%, is 293mg/L all the time; The C stage is that total freeing port went out current 6 hours, and the response variation in 4.4%, is 53.8mg/L all the time; The D stage flowed through 12 hours for the oil interceptor water outlet, and response changes in 1.9%, is 232.2mg/L; The E stage is that the water outlet of 1# flotation cell was flowed through 8 hours, and response changes in 1.7%, is 207.8mg/L; The F stage flowed through 12 hours for the oil interceptor water outlet, and the response change value is 414.8mg/L between 3.5%.The measured value in each stage is more consistent with the classic method value of measuring.In a word, stable for the response of five types that are surveyed waste water, disturb very for a short time, changing value is no more than 5%, has proved the feasibility that works online.
Table 1 is the on-line determination experimental data.
In the on-line determination, at first,, directly reflect the change of water quality of waste water by the situation of change of response current then with the continuous flow injection analysis incessantly in 48 hours of various types of waste water with the standard GGA standardization of solution work system of 16.5mg/L.As seen, BOD sensor continuous working 48 hours is stablized for the response of various concentration waste water in the table, and the long period, interior variation was very little, and approaching with the classic method measurement result.Illustrate that available this kind method carries out the continuous monitoring of waste water.
| Sample | Extension rate | Changing value | BOD s | BOD 5 |
| GGA | 1 | 2.7％ | 16.5 | 16.5 |
| The water outlet of 1 | 20 | 2.4％ | 293.3 | 205 |
| Total freeing | 2 | 4.4％ | 53.8 | 95 |
| The oil | 20 | 1.9％ | 232.2 | 180 |
| The water outlet of 1 | 20 | 1.7％ | 207.8 | 175 |
| The oil | 20 | 3.5％ | 414.8 | 306 |
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| CN 00116096 CN1118705C (en) | 2000-10-25 | 2000-10-25 | Method and device for on-line monitoring waste water |
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| CN 00116096 CN1118705C (en) | 2000-10-25 | 2000-10-25 | Method and device for on-line monitoring waste water |
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| CN1290859A true CN1290859A (en) | 2001-04-11 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101206191B (en) * | 2006-12-20 | 2011-12-14 | 株式会社东芝 | Biosensor type exception water quality monitoring apparatus |
| CN101241103B (en) * | 2007-02-08 | 2012-03-21 | 株式会社东芝 | Abnormal water detecting device and poison response sensitivity prediction method thereof |
| CN105137015A (en) * | 2014-12-10 | 2015-12-09 | 中国科学院城市环境研究所 | Water-quality biological-pollution on-line monitoring mechanism |
| US11714030B2 (en) * | 2018-07-31 | 2023-08-01 | Biobot Analytics, Inc. | Modular system for upstream wastewater sampling |
-
2000
- 2000-10-25 CN CN 00116096 patent/CN1118705C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101206191B (en) * | 2006-12-20 | 2011-12-14 | 株式会社东芝 | Biosensor type exception water quality monitoring apparatus |
| CN101241103B (en) * | 2007-02-08 | 2012-03-21 | 株式会社东芝 | Abnormal water detecting device and poison response sensitivity prediction method thereof |
| CN105137015A (en) * | 2014-12-10 | 2015-12-09 | 中国科学院城市环境研究所 | Water-quality biological-pollution on-line monitoring mechanism |
| US11714030B2 (en) * | 2018-07-31 | 2023-08-01 | Biobot Analytics, Inc. | Modular system for upstream wastewater sampling |
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| Publication number | Publication date |
|---|---|
| CN1118705C (en) | 2003-08-20 |
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