CN1246687C - A device combining a point light source with a single lens - Google Patents

Abstract

A device combining a point light source and a single lens. The device of the present invention mainly includes a point light source, a light sensor and a lens. The lens is arranged on the same side of the point light source and the light sensor so that the emitted light of the point light source is focused on an object to be measured through the lens, and the reflected light of the object to be measured is focused on the light sensor through the lens. With the aid of the device of the present invention, the content of a specific component of a test object can be determined qualitatively and quantitatively, and the measurement clarity can be improved by the combination of the point light source, the light sensor and the single lens.

Description

A device combining a point light source with a single lens

(1) Technical field

The invention relates to a device capable of detecting a test object with a small detection area; in particular, it relates to a device combining a point light source and a single lens.

(2) Background technology

The quantification of chemical and biochemical components in chromogenic aqueous liquids, especially in chromogenic biological fluids such as blood, urine and derivatives of biological fluids such as serum and plasma, has become more and more important. Many important applications already exist in medical diagnosis and treatment, as well as in the quantification of therapeutic drugs, toxic substances and harmful chemicals. In some applications, the concentration of the substance that needs to be quantified is often in the range of mg/100 cm3 or below, which is too small, so that it is difficult to quantify accurately, or requires the use of complex instruments and skilled operators. Also, in such cases, analytical results are usually not available until hours or days after sampling.

A common medical test is the measurement of blood sugar levels in diabetic patients. The current practice is to recommend that diabetic patients measure their blood sugar levels two to seven times a day according to their individual conditions. Diabetic patients and doctors then adjust the diet, exercise and insulin injections of diabetic patients according to the blood sugar measurement results, so as to effectively control the diabetes condition.

A kind of blood sugar level detection method widely used now is to adopt a kind of test strip, apply blood sample (about 20 to 40 microliters) on the test strip coated with ethyl cellulose on a reagent pad, this The reagent pad contains an enzyme system including glucose oxidase and peroxidase. This enzyme system reacts with glucose to produce hydrogen peroxide. The reagent pad still contains an indicator that, in the presence of peroxidase, reacts with hydrogen peroxide to produce a chromogenic substance whose color intensity is directly proportional to the glucose concentration in the blood sample. Another blood sugar detection method uses the same chemical reaction principle, but replaces the reagent gasket coated with ethyl cellulose with a waterproof film, wherein the enzyme components and indicators are dispersed in the waterproof film.

In the above two known methods for measuring blood sugar levels, the blood sample is in contact with the reagent pad for a predetermined time, about several minutes. Then, with respect to the above-mentioned first known method, the blood sample is washed off the reagent pad with clear water, and with respect to the above-mentioned second known method, the blood sample is wiped off the reagent pad. Next, the reagent pads were blotted dry and assayed. This method of determination is to compare the color intensity of the reagent pad with a color chart, or place the test piece in a reflectance measuring instrument to read a color intensity value on the reagent pad .

Although the above-mentioned methods have been used in the detection of blood sugar levels for many years, these methods have their limitations. For example, in terms of fingertip sampling, a larger sample detection area is required. For people whose microvessels are not obvious In other words, it is difficult to obtain sufficient blood samples.

The quantitative results of the above-mentioned known detection methods depend on the read color intensity value, and the color intensity value is related to the degree of reaction between the blood sample and the detection reagent on the reagent pad. The first known method is to wash or wipe off the blood sample from the reagent pad after a predetermined reaction time, so the user needs to be prepared to wash off the reagent pad with water after the predetermined reaction time. blood samples or swabs of blood samples. The removal of the blood sample from the reagent pad will stop the reaction of the blood sample with the detection reagent on the reagent pad, resulting in uncertainty of the test results. Excessive cleaning may result in lower detection results, while insufficient cleaning may result in higher detection results , for users who use it at home by themselves, this problem is more obvious.

Another problem is that a time sequence needs to be initiated when the blood sample is applied on the reagent pad. The user usually takes the blood sample by pricking the fingertip, and the user needs to use the other hand to start the timer while applying the blood sample on the reagent pad. Therefore, the above-mentioned known detection method requires the user to use both hands at the same time. Since the timer has to be started only when the blood sample is applied to the reagent pad, it becomes difficult for the user to operate.

Accordingly, it is urgent to provide a device capable of detecting the content of specific components in chromogenic liquids such as blood samples, which can overcome the shortcomings of conventional detection methods.

(3) Contents of the invention

The main purpose of the present invention is to provide a device combining a point light source and a single lens, which can detect point-shaped or small detection objects.

Another object of the present invention is to provide a device combining a point light source and a single lens, which is light, thin, short, small and miniaturized, and is easy to carry.

Another object of the present invention is to provide a device combining a point light source and a single lens, wherein the lens has a light-condensing effect without requiring a high-intensity light source.

Another object of the present invention is to provide a device combining a point light source and a single lens, which has few and simple components, is easy to manufacture, and can save manufacturing cost.

Yet another object of the present invention is to provide a device combining a point light source and a single lens, which can be used to qualitatively and quantitatively detect a specific component in a test area of a test strip.

Still another object of the present invention is to provide a device combining a point light source and a single lens, which can be used to detect a specific component in a biological sample, and is easy to operate, which is beneficial to the user's self-detection.

Yet another object of the present invention is to provide a device combining a point light source and a single lens, which can improve measurement clarity through the light-gathering effect of the lens.

A device combining a point light source and a single lens, comprising: a point light source; a light sensor; and a lens, which is arranged on the same side of the point light source and the light sensor, so that the emitted light of the point light source is focused through the lens on an object to be tested, and the reflected light of the object to be tested is focused on the light sensor through the lens; the object to be tested is a test piece comprising a light-absorbing area, and the light-absorbing area is given by the A specific component of a tested sample of the test strip is produced, and the light absorption area can absorb the emitted light of the point light source; the feature is that the point light source can emit light of a first wavelength and a second wavelength light, wherein the light of the first wavelength can be absorbed by the test sample contained in the light absorption area of the test piece, and the intensity of the light of the first wavelength reflected by the light absorption area is used to determine the The sample amount of the test object of the test strip, and the second wavelength is absorbed by the light absorption area corresponding to the specific component of the test sample, and by means of the second wavelength reflected by the light absorption area The intensity of the wavelength of light to determine the concentration of that particular component.

The lens has a secondary light-gathering effect, and the measurement clarity of the device of the invention can be improved by virtue of the combined relationship of the point light source, the light sensor and the lens.

The device combining a point light source and a single lens provided by the present invention has few and simple components, can save space and is easy to carry, and can reduce manufacturing costs.

The objectives and advantages of the present invention will be more apparent with the detailed description of the following specific embodiments and with reference to the accompanying drawings.

(4) Description of drawings

Figure 1 is a schematic perspective view of a test piece; and

FIG. 2 is a functional block diagram of a light reflectance measuring instrument including a preferred embodiment of the present invention.

(5) specific implementation

The invention provides a device combining a point light source and a single lens, which mainly includes a point light source, a light sensor and a lens. The lens is arranged on the same side of the point light source and the light sensor, so that the emitted light of the point light source is focused on an object under test through the lens, and the reflected light of the object under test is focused on the light sensor through the lens. The device of the present invention can be used as a light reflectance measuring instrument, which can be applied to detect objects with point-shaped detection areas or small detection areas, and determine the object to be measured by measuring the reflected light intensity reflected by the object to be measured. The amount of a specific ingredient in it. The device of the present invention can be used to measure the reflected light intensity of a color-developed area on a test strip, and determine the content of a specific component in a tested sample tested by the test strip by the measured reflected light intensity.

Referring to FIG. 1 , it is a schematic perspective view of a test strip 10 for detecting a specific component in a test sample. The test strip 10 includes a strip substrate 12 and a reagent gasket 14 . The reagent gasket 14 is pasted on one end of a test surface of the strip substrate 12 by an adhesive 16 . The reagent gasket 14 includes an enzyme system and an indicator (indicator), wherein the enzyme system contains an oxidase that can cause the specific component in the test sample to react to produce hydrogen peroxide and react the hydrogen peroxide with the indicator of peroxidase. The peroxidase in the enzyme system can make hydrogen peroxide react with the indicator to produce a color-absorbing substance. An opening 18 is provided on a sample surface of the strip substrate 12 opposite to the test surface, through which the sample to be tested is applied to the reagent pad 14 . When the enzyme system of the reagent gasket 14 contains glucose oxidase and peroxidase, the glucose in the blood sample given to the reagent gasket 14 through the opening 18 on the sample surface is catalyzed by glucose oxidase to generate hydrogen peroxide, and Under the catalysis of peroxidase, the hydrogen peroxide reacts with the indicator to produce a color-developing and light-absorbing substance, which is formed on a pair of sides of the test surface of the strip substrate 12 corresponding to the reagent gasket 14 . The glucose concentration in the blood sample is proportional to the color intensity of the color-absorbing substance. This color-developing light-absorbing substance will change the light reflection intensity of the reagent gasket 14, and by measuring the reflected light intensity of the color-developing light-absorbing substance, the glucose concentration in the blood sample can be determined. When the enzyme system of the reagent pad 14 contains cholesterol oxidase, which can catalyze cholesterol to generate hydrogen peroxide, the test strip 12 can be used to detect the cholesterol content in the blood sample. Therefore, by changing the composition of the enzyme system of the test strip 12, the specific composition of the test sample that can be detected can be determined. In addition, the light reflection intensity of the color-developing light-absorbing substance on the reagent pad 14 is inversely proportional to the concentration of a specific component in the test sample. The light reflection intensity of the color-developing and light-absorbing substance can determine the concentration of this specific component in the tested sample.

The device of the present invention and its application will be described in detail below through preferred specific embodiments.

With reference to Fig. 2, it is the functional block diagram that comprises a light reflectance measuring instrument of a preferred embodiment of the present invention, and this embodiment comprises a holder 21, a point light source 22, a light sensor 23, a lens 24, a Amplifier 25 , an analog-to-digital converter 26 , a microprocessor 27 , a memory and a display 29 . The point light source 22 is disposed at a first end of the holder 21 , and the light sensor 23 is disposed at a second end of the holder 21 on the same side as the first end. The lens 24 is disposed on the same side of the point light source 22 and the light sensor 23 , so that the emitted light of the point light source 22 is focused on a light absorption area 31 of a test object 30 on the other side of the lens 24 through the lens 24 . In the present invention, the object under test 30 is preferably located at the focal length of the lens 24 . The reflected light from the light absorption area 31 of the object under test 30 is then focused on the light sensor 23 through the lens 24 . The light-absorbing area 31 of the object under test 30 includes a color-developing light-absorbing substance, which can absorb the light emitted by the point light source 22 . The color intensity of the color-developing light-absorbing substance is directly proportional to the content of a specific component that produces the color-developing light-absorbing substance, so that the light reflection intensity of the color-developing light-absorbing substance to the point light source 22 is inversely proportional to the specific component. . Referring again to FIG. 1 , the test object 30 can be the test strip 10 including a light-absorbing substance, and the light-absorbing substance is produced by a specific component of a test sample applied to the test strip 10 . The light sensor 23 generates a response current corresponding to the reflected light passing through the light absorption area 31 of the object under test 30 . In this preferred embodiment, the point light source 22 can be a light emitting diode (light emitting diode), and the light sensor 23 can be a photodiode (photodiode), a charge-coupled device (charge-coupled device) and a complementary Metal-Oxide-Semiconductor sensor (Complementary Metal-Oxide-Semiconductor sensor).

An induced current generated by the light sensor 23 corresponding to the reflected light intensity reflected by the light absorption area 31 of the object under test 30 is converted into a voltage through an amplifier 25, and then converted into a set of digital signals through an analog-to-digital converter 26, and then sent to to a microprocessor 27. The microprocessor 27 can provide the following functions: timing the measurement of the light reflectance measuring instrument; reading the digital signal output by the analog-to-digital converter 26; combining with an execution program stored in the memory 28, to calculate a sampling time point The light reflection intensity value is stored in the memory 28; according to the stored light reflection intensity value, a specific component concentration value corresponding to the light absorption area 31 of the object under test 30 is determined. The concentration value is then displayed via a display 29 such as a liquid crystal display.

In another preferred embodiment of the present invention, except for the point light source, all other components are the same as those shown in FIG. 2 . The point light source 22 is designed to emit a first wavelength light and a second wavelength light Wherein the light of the first wavelength can be absorbed by a test object contained in the light absorption area 31 of the object to be tested 30, and the intensity of the light of the first wavelength reflected by the light absorption area 31 can be determined to be applied to the object to be tested 30. Whether the sampling volume of the tested sample is sufficient. For example, when the sample amount of the test sample administered to the light absorption area 31 of the test object 30 is not enough, the light reflection intensity of the first wavelength of light can be measured compared to the time when the test sample is not administered. Slightly reduced, for example, when the light reflection intensity of the first wavelength light is not lower than a predetermined value, the analog-to-digital converter 26 can send a one-bit digital signal "0" to the microprocessor 27, and then the microprocessor 27 sends a warning Signal to remind the user that the sample volume of the tested sample is insufficient. When the sample amount of the tested sample given to the light absorption area 31 of the test object 30 is sufficient, it can be measured that the light reflection intensity of the first wavelength is significantly lower than that when it is not given to the tested sample, For example, the light reflection intensity of the light with the first wavelength is lower than the predetermined value, at this time, the analog-to-digital converter 26 can send a one-bit digital signal “1” to the microprocessor 27 . The microprocessor 27 then controls the point light source 22 to emit light with a second wavelength, which is focused on the light-absorbing area 31 of the object under test 30 through the lens 24. The second-wavelength light can be absorbed by a color-developing light-absorbing substance contained in the light-absorbing area 31 , and this chromogenic light-absorbing substance is caused by a specific component in the tested sample. The specific component concentration can be determined by the intensity of the light of the second wavelength reflected by the light absorption area 31 .

The device combining a point light source and a single lens provided by the present invention can be applied to a light reflectance measuring instrument, wherein the lens has a secondary light-gathering effect, which can improve the measurement clarity of the light reflectance measuring instrument. The device of the present invention has few and simple components. In addition to achieving the purpose of reducing manufacturing costs, it also meets the needs of lightness, thinness, shortness, smallness and miniaturization, and is convenient for users to carry.

The above description is only a specific embodiment of the present invention, and it is not intended to limit the patent scope of the present invention; all other equivalent changes or equivalent replacements that do not deviate from the spirit disclosed by the present invention should be included in the following within the scope defined by the claims.

Claims (9)

1. the device of binding site light source and single lens comprises:

One pointolite;

One optical sensor; And

One lens are homonymies of being located at this pointolite and this optical sensor so that the emission light of this pointolite via this lens focus in a determinand, and the reflected light that makes this determinand via this lens focus in this optical sensor; Described determinand is a test piece that comprises a light absorption area, and this light absorption area is that this light absorption area can absorb this emission light of this pointolite by a specific composition generation of a tested corpse or other object for laboratory examination and chemical testing that is administered to this test piece; It is characterized in that:

Described pointolite can be launched one first wavelength light and one second wavelength light, wherein this first wavelength light is to be absorbed by this tested corpse or other object for laboratory examination and chemical testing that this light absorption area of this test piece comprises, reach with the intensity of this first wavelength light that reflects through this light absorption area, bestow sampling amount with decision at this tested corpse or other object for laboratory examination and chemical testing of this test piece, and this second wavelength is by this light absorption area absorption that should specific composition produces that mutually should a tested corpse or other object for laboratory examination and chemical testing, reach with the intensity of this second wavelength light that reflects through this light absorption area, to determine the concentration of this specific composition.

2. the device of binding site light source as claimed in claim 1 and single lens is characterized in that, also comprises a retaining piece, is used to set firmly this pointolite in one first end of this retaining piece and set firmly one second end of this optical sensor in this retaining piece.

3. the device of binding site light source as claimed in claim 1 and single lens is characterized in that, described determinand is a focal position that is positioned at these lens.

4. the device of binding site light source as claimed in claim 1 and single lens is characterized in that described pointolite comprises a light emitting diode.

5. the device of binding site light source as claimed in claim 1 and single lens is characterized in that, described optical sensor is that corresponding reflected light through this determinand produces an induction current.

6. the device of binding site light source as claimed in claim 5 and single lens is characterized in that, described optical sensor is to be selected from one of photodiode, charge coupled cell and CMOS (Complementary Metal Oxide Semiconductor) sensor.

7. the device of binding site light source as claimed in claim 1 and single lens is characterized in that, this specific composition of this tested corpse or other object for laboratory examination and chemical testing that described test piece detects is the ferment system decision that is comprised by this test piece.

8. the device of binding site light source as claimed in claim 7 and single lens is characterized in that, described test piece is in order to detect the concentration of glucose of a blood sample.

9. the device of binding site light source as claimed in claim 7 and single lens is characterized in that, described test piece is in order to detect the cholesterol level of a blood sample.

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