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CN1243543A - 通过交叉流过滤纯化或/和浓缩dna,从核酸制品中分离内毒素 - Google Patents

通过交叉流过滤纯化或/和浓缩dna,从核酸制品中分离内毒素 Download PDF

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Publication number
CN1243543A
CN1243543A CN98801764A CN98801764A CN1243543A CN 1243543 A CN1243543 A CN 1243543A CN 98801764 A CN98801764 A CN 98801764A CN 98801764 A CN98801764 A CN 98801764A CN 1243543 A CN1243543 A CN 1243543A
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CN
China
Prior art keywords
nucleic acid
solution
described method
purifying
cross
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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CN98801764A
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English (en)
Chinese (zh)
Inventor
W·库恩
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Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Publication of CN1243543A publication Critical patent/CN1243543A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CN98801764A 1997-01-10 1998-01-09 通过交叉流过滤纯化或/和浓缩dna,从核酸制品中分离内毒素 Pending CN1243543A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97100331A EP0853123A1 (de) 1997-01-10 1997-01-10 Reinigung von DNA durch Cross-Flow-Filtration
EP97100331.4 1997-01-10

Publications (1)

Publication Number Publication Date
CN1243543A true CN1243543A (zh) 2000-02-02

Family

ID=8226362

Family Applications (1)

Application Number Title Priority Date Filing Date
CN98801764A Pending CN1243543A (zh) 1997-01-10 1998-01-09 通过交叉流过滤纯化或/和浓缩dna,从核酸制品中分离内毒素

Country Status (8)

Country Link
EP (2) EP0853123A1 (de)
JP (1) JP2000513580A (de)
KR (1) KR100322398B1 (de)
CN (1) CN1243543A (de)
BR (1) BR9807062A (de)
CA (1) CA2277165C (de)
TR (1) TR199901840T2 (de)
WO (1) WO1998030685A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103173438A (zh) * 2013-04-10 2013-06-26 杭州百迈生物技术有限公司 一种提取质粒的试剂和方法

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GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
GB2337261B (en) * 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids
DE19900681B4 (de) 1999-01-04 2007-04-12 Sartorius Ag Verwendung von Anionaustauschermembranen zur Entfernung von Endotoxinen aus Nukleinsäure-haltigen Lösungen
ES2218185T3 (es) * 1999-07-23 2004-11-16 Genentech, Inc. Metodo para la purificacion de adn plasmidico exento de rnasa y de solvente organico, usando filtracion de flujo tangencial.
US6284117B1 (en) * 1999-12-22 2001-09-04 Nanogen, Inc. Apparatus and method for removing small molecules and ions from low volume biological samples
DE10006591B4 (de) * 2000-02-11 2007-03-29 Eppendorf Ag Verfahren zur Aufreinigung von Nukleinsäuren
DE10022258A1 (de) * 2000-05-08 2001-11-15 Bayer Ag Reinigung von Proteineinschlusskörpern durch Querstrom-Mikrofiltration
WO2001094573A2 (en) * 2000-06-02 2001-12-13 Pall Corporation Processing of plasmid-containing fluids
KR100356738B1 (ko) * 2000-09-07 2002-10-18 주식회사 삼양제넥스 염기성 단백질로부터 엔도톡신을 제거하는 방법
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
JP2005538929A (ja) 2002-01-16 2005-12-22 ダイナル バイオテック エイエスエイ 単一サンプルからの核酸及びタンパク質の単離方法
EP1554398A4 (de) 2002-09-13 2005-12-14 Valentis Inc VORRICHTUNG UND VERFAHREN ZUR REINIGUNG VON NUKLEINSûUREN IM PRûPARATIVEN MASSSTAB
WO2004048398A1 (ja) * 2002-11-28 2004-06-10 Arkray Inc. 核酸の濃縮精製方法および装置
US7238522B2 (en) 2003-05-30 2007-07-03 Advisys, Inc. Devices and methods for biomaterial production
US8075780B2 (en) * 2003-11-24 2011-12-13 Millipore Corporation Purification and concentration of synthetic biological molecules
WO2010009415A1 (en) * 2008-07-18 2010-01-21 Canon U.S. Life Sciences, Inc. Methods and systems for microfluidic dna sample preparation
AU2010326132B9 (en) 2009-12-01 2014-10-02 Translate Bio, Inc. Delivery of mRNA for the augmentation of proteins and enzymes in human genetic diseases
EP4458350A3 (de) 2011-06-08 2025-01-22 Translate Bio, Inc. Lipidnanopartikelzusammensetzungen und verfahren zur mrna-abgabe
US20150267192A1 (en) 2012-06-08 2015-09-24 Shire Human Genetic Therapies, Inc. Nuclease resistant polynucleotides and uses thereof
HUE042640T2 (hu) 2013-03-14 2019-07-29 Translate Bio Inc CFTR-mRNS-készítmények, valamint kapcsolódó eljárások és alkalmazások
EP2970955B1 (de) * 2013-03-14 2018-11-14 Translate Bio, Inc. Verfahren zur reinigung von messenger-rna
DK2970948T3 (en) 2013-03-15 2019-04-08 Glaxosmithkline Biologicals Sa METHODS OF RNA PURIFICATION
AU2014340092B2 (en) 2013-10-22 2019-09-19 Translate Bio, Inc. mRNA therapy for Argininosuccinate Synthetase Deficiency
CA2928186A1 (en) 2013-10-22 2015-04-30 Shire Human Genetic Therapies, Inc. Mrna therapy for phenylketonuria
DK178664B1 (en) * 2013-10-25 2016-10-24 Amphi Consult V Martin Hesselsøe Aps A system and a method for concentrating traces of tissue from aquatic organisms in a water sample and use thereof
US20170173537A1 (en) * 2014-03-07 2017-06-22 Agency For Science, Technology And Research Apparatus and methods for fractionation of biological products
PT3134506T (pt) 2014-04-25 2019-10-31 Translate Bio Inc Métodos de purificação de rna mensageiro
DK3294885T3 (da) 2015-05-08 2020-08-10 Curevac Real Estate Gmbh Fremgangsmåde til at fremstille rna
US10760070B2 (en) * 2015-05-29 2020-09-01 Curevac Real Estate Gmbh Method for producing and purifying RNA, comprising at least one step of tangential flow filtration
MX2019010155A (es) 2017-02-27 2020-12-10 Translate Bio Inc Arnm de cftr optimizado por codón novedoso.
US11173190B2 (en) 2017-05-16 2021-11-16 Translate Bio, Inc. Treatment of cystic fibrosis by delivery of codon-optimized mRNA encoding CFTR
CN112930396B (zh) 2018-08-24 2024-05-24 川斯勒佰尔公司 用于纯化信使rna的方法
AU2019384557A1 (en) 2018-11-21 2021-06-10 Translate Bio, Inc. Treatment of cystic fibrosis by delivery of nebulized mRNA encoding CFTR

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2705251B1 (fr) * 1993-05-13 1995-07-07 France Etat Armement Détermination de substances dans un milieu liquide par filtration tangentielle.
CA2192342A1 (en) * 1994-07-15 1996-02-01 Ann L. Lee A method for large scale plasmid purification
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103173438A (zh) * 2013-04-10 2013-06-26 杭州百迈生物技术有限公司 一种提取质粒的试剂和方法

Also Published As

Publication number Publication date
EP0853123A1 (de) 1998-07-15
JP2000513580A (ja) 2000-10-17
WO1998030685A2 (de) 1998-07-16
KR20000069943A (ko) 2000-11-25
KR100322398B1 (ko) 2002-03-18
AU723817B2 (en) 2000-09-07
EP0961826A2 (de) 1999-12-08
TR199901840T2 (xx) 2000-02-21
CA2277165C (en) 2004-03-30
WO1998030685A3 (de) 1998-10-22
AU6208498A (en) 1998-08-03
CA2277165A1 (en) 1998-07-16
BR9807062A (pt) 2000-05-02

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