CN1240827C - Culture medium of salt water for preserving and multiplying fungus in use for chicken eggs containing potassium tellurite - Google Patents

Abstract

The present invention relates to a culture medium prepared from potassium tellurite, eggs and saline for preserving and multiplying fungi, which is applied to bacterial (corynebacterium diphtheriae) examinations of microbiological examination aspects in medical microbiology. The culture medium which is a liquid culture medium is prepared from eggs, physiological saline and a potassium tellurite solution; protein in the culture medium has the dual functions of prevention of the death of target fungi and nutrition, and potassium tellurite can prevent non-target fungi from being multiplied, and simultaneously has the functions of preserving and multiplying fungi. The present invention solves the problems of death of target fungi and damage of sampling equipment, enhances the examination rate of target fungi, and reduces labor intensity.

Description

Potassium tellurite egg salt solution is protected the bacterium enrichment medium

Technical field

The present invention relates to the substratum of " Micro biological Tests " aspect in a kind of being applied to " medical microbiology " " pathogenic bacteria (diphtheria corynebacterium) " check.

Background technology

Before this technological invention, the check of diphtheria corynebacterium is a throat swab of gathering patient, patient suspected or carrier, with throat swab direct inoculation potassium tellurite blood agar plate substratum or egg slant medium or Lv Shi serum slant medium.Egg slant medium or Lv Shi serum slant medium are pure egg liquid or serum to be heated be frozen into ramped shaped in test tube, and the potassium tellurite blood agar plate is with dissolvings such as peptone, glucose, beef extract, rabbit blood and sneaks into distilled water and add the flat substratum that the peptizer agar powder is made again.There are some shortcomings in these substratum: the one, the preparation of egg slant medium or Lv Shi serum slant medium is very loaded down with trivial details, need divide sterilization in three days three times under 85 ℃ wet heat condition, after each sterilization with culture medium culturing 24 hours; The 2nd, after usefulness egg slant medium or Lv Shi serum slant medium are cultivated sample, assorted bacterium is too many, and each sample all needs to carry out microscopy with after gramstaining and the Babes-Ernst bodies dyeing, workload is big especially, can't be used for the above large sample test of tens people; The 3rd, potassium tellurite blood agar plate culture volume is big, measure to be difficult to transportation when big, and glass dish damages easily when transportation; The 4th, above-mentioned these substratum must be cultivated behind seed sample immediately, have cultivated with regard to inconvenience when the laboratory is far away far from the sampling location, and at this moment the bacterium in the sample will be dead gradually, and reduced the later possibility that detects the purpose bacterium; The 5th, above-mentioned these substratum all are solid mediums, and they only produce single bacterium colony during to microbial culture, do not have the bacterium of increasing effect, so bacteria containing amount more just can't detect in the sample.

Summary of the invention

For overcoming the deficiencies in the prior art part, the object of the present invention is to provide a kind of make simple, easy to use, easily obtain result's novel culture medium.

For achieving the above object, the technical scheme that the present invention takes is: the albumen of utilization in the egg prevents the death of purpose bacterium and a large amount of breedings that nutrition dual function, potassium tellurite prevent non-purpose bacterium, makes liquid nutrient medium into, make it have the bacterium of guarantor, increase the bacterium dual function, before cultivation, increase bacterium earlier, carry out separation and Culture again after increasing bacterium.Through experimental study, we have determined to protect the prescription of bacterium enrichment medium and the volume percent of each composition is: egg: physiological saline 75%: 1% potassium tellurite solution 23%: 2%.

Because this substratum has the effect of protecting bacterium, increasing bacterium simultaneously, some beneficial effects have been obtained: when remote sampling, can avoid the death of purpose bacterium, before formal check, make the amplification in advance of purpose bacterium, increase the bacteria containing amount of purpose bacterium in the sample, can improve the recall rate of purpose bacterium.Solved transportation problem after away from breadboard spot sampling, can prevent the death of purpose bacterium, the breakage of the equipment that prevents to sample, in sample the very low recall rate that can improve the purpose bacterium of purpose bacterial content, reduced and carried out microscopy after labour intensity one when great amount of samples is checked need not each sample all used gramstaining and Babes-Ernst bodies dyeing, need not to prepare loaded down with trivial details egg slant medium or Lv Shi serum slant medium.

Embodiment

Preparation potassium tellurite solution: take by weighing analytical pure potassium tellurite 1 gram, be dissolved in 100 ml distilled waters, the sterilization in 15 minutes of water proof heated and boiled, the cooling back is standby.

Preparing normal saline: take by weighing analytical pure sodium-chlor 0.85 gram, be dissolved in 100 ml distilled waters, pack in the clean saline bottle, build, sterilized 15 minutes for 121 ℃, the cooling back is standby.

Preparation is beaten eggs and filters: get one of 1000 milliliters of clean Erlenmeyer flask, 100 of the granulated glass spherees clean, diameter 5 millimeter of packing into seal; Other gets gauze a slice, is folded into 8 layers (length of side is more than 15 centimetres), puts into instruments box with glass funnel, wraps; Wrap several 1000 clean several of ml flasks again, 15 * 150 millimeters test tube suction pipes some, 10 milliliters are several, all put into pressure kettle, 121 ℃ of sterilizations 15 minutes, cooling, oven dry back are standby.

The sterilization egg: it is some to get fresh hen egg, and it is soaked in 75% the ethanol for disinfection, soaks band handle metal ring (making with thick long iron wire) and antimagnetic type tweezer simultaneously, soaks 5 minutes.

Preparation egg liquid: take out an egg with the metal ring after soaking, in the hole that the major part of egg is opened about 3 centimetres of diameters lightly, entire contents is poured in the Erlenmeyer flask with little granulated glass sphere of sterilization with tweezers.Till repetitive operation is extremely enough.Seal the mouth of Erlenmeyer flask, use forced oscillation, all smash, stir evenly until yolk.The egg smashing, stir evenly is packed into after with filtered through gauze in the another Erlenmeyer flask, build standby.

Preparation potassium tellurite egg salt water culture medium: 92 milliliters of 300 milliliters of egg liquids, sterile salines, 8 milliliters of the sterilization potassium tellurite solution of getting above-mentioned preparation, fully behind the mixing, be sub-packed in the aseptic test tube with 10 milliliters of aseptic suction pipes, 2 milliliters of every pipes can use after having filled in test tube according to a conventional method.

Claims (1)

1. a potassium tellurite egg salt solution is protected the bacterium enrichment medium, and described substratum is a kind of liquid nutrient medium, and the volume percent of its prescription and each composition is: the potassium tellurite solution 2% of egg liquid 75%, physiological saline 23%, 1%.