CN1234009C - Microflow controlled chip flow-type biochemical analysis instrument and method for detecting biochemical components - Google Patents

Abstract

The microfluidic chip flow type biochemical analyzer and the method for detecting biochemical components of the present invention belong to the detection and analysis instrument and the detection method. The microfluidic chip 14 is made of two layers of thin plates bonded by etching, and a cross channel 21 is engraved between the thin plates. The liquid outflow hole 20 is connected with the outside world. One electrode is inserted into each of the four holes. The laser light emitted by the laser light source 1 is focused on the detection point 24 of the cross channel; the fluorescence emitted by the substance in the channel 21 is received by the optical fiber 15 and transmitted to the spectroscopic detection system 16 composed of a CCD spectral detector. The invention is small in size, light in weight, compact in structure, simple in operation, less in sample sheath fluid consumption, can choose fluorescent reagents at will, obtain fluorescence intensity of multiple wavelengths at the same time, and has fast response speed, and can be used for analysis of biochemical substances and chemical components , drug screening, clinical diagnosis and other fields.

Description

Microfluidic chip flow biochemical analyzer and its application in the detection of biochemical components

technical field

The invention belongs to a biochemical analyzer used in biochemistry, pharmaceutical research, biomedical research and clinical diagnosis, etc. and a method for detecting nucleic acid, antibody or antigen, peptides and chemical factors using the biochemical analyzer.

Background technique

The prior art close to the present invention is organic fluorescent dye-encoded microspheres and flow cytometry based on fluorescent dye-encoded microspheres. Quantitative flow cytometry based on fluorescent dye-labeled microspheres has been used to accurately quantify cell surface receptors, etc., and the results are comparable to traditional Scatchard assays and can be used for inter-laboratory standardized studies, As a result, several companies have started to produce high-quality fluorescent calibration beads.

Instruments that combine organic fluorescent dye-encoded microspheres with traditional flow cytometers for simultaneous detection of multiple components are already on the market, such as the products of Luminex in the United States. The structural principle of the flow cytometer is shown in Fig. 1 . It is mainly composed of a laser light source 1, a flow chamber 2, a spectroscopic device and a detection system. The flow chamber 2 is a small chamber where the sample and the sheath fluid (buffer) are mixed, usually made of plexiglass, optical glass or quartz, and is the heart of the flow system. The detected sample particles (cells or microspheres) and the sheath fluid flow respectively enter the flow tube 5 of the flow chamber 2 from the sample inflow hole 3 and the sheath fluid inflow hole 4 and are irradiated by the focused laser. The air pressure sampling method is used to make the sample particles to be detected and the sheath fluid flow. The spectroscopic device includes two dichroic mirrors 6 . The specific optical signal related to the sample particle is reflected or transmitted by the dichroic mirror 6 according to the wavelength of the light, splits the light, passes through the band-pass filter 7 and the convex lens 8, and finally reaches the detection system. The detection system includes a photomultiplier tube 9 and a silicon photodiode 10, two photomultiplier tubes 9 and a silicon photodiode 10 respectively receive two beams of reflected light and transmitted light and detect the intensity thereof.

Because the flow chamber 2 is exquisite in workmanship and accurate in size, it is required to be very stable in fluid mechanics, optics, mechanics and electricity in design, so it has the disadvantages of difficult processing, large volume and high price. At the same time, due to the method of air pressure sampling, a large amount of sheath fluid is required, which further increases the difficulty of miniaturization. Since the photomultiplier tube 9 and the silicon photodiode 10 are used as the detector, and the dichroic mirror 6 is used as the spectroscopic device, the optical structure is quite complicated, the adjustment is also quite difficult, and the operation is complicated, requiring specially trained personnel to operate. And only a few specific emission wavelengths can be detected. The more types of wavelengths, the higher the price, and the larger the volume of the instrument.

The sample particles are microspheres encoded with fluorescent dyes. Because the excitation spectrum and emission spectrum of organic dyes are relatively close, and the peak shape is asymmetric, the emission peak "smears" seriously, the brightness is low, and the photofading phenomenon is serious. And due to the problem of light fading, this kind of labeled microspheres is unstable and difficult to store. And it is difficult to choose the same excitation light source to excite several fluorescent dyes (generally <3 types) at the same time, so it is difficult to use them to mark a large number of microspheres. At present, there are 64 types of labeled microspheres on the market, and it is said that there will be 100 types. Microspheres with different codes are coming soon, but this is still far from enough compared with the total of about 30,000 to 40,000 human genes.

Contents of the invention

The technical problem to be solved in the present invention is to overcome the deficiencies of the background technology, design a flow biochemical analyzer and a method for detecting biochemical components with quantum dot coded microspheres, so that the overall performance of the instrument is stable, and the volume of the flow chamber and the detector is reduced. Small, the amount of sample to be tested and the amount of sheath fluid are reduced, the price is low, the light intensity at all wavelengths can be detected, and the data at each wavelength can be obtained at one time; the coded microspheres used have high brightness, no fading, and are easy to store , more kinds of coded microspheres can be excited simultaneously with the same excitation light source, so that more kinds of biological or chemical components can be detected simultaneously and rapidly.

The structure of the microfluidic chip flow biochemical analyzer of the present invention mainly includes: a laser light source, a microfluidic chip analysis system, and a spectroscopic detection system (CCD full spectrum instrument).

The so-called microfluidic chip is made of two layers of thin plates after etching and bonding. The thin plates can be made of glass or plastic or other materials. There are cross channels engraved between the two layers of thin plates. Holes, two opposite buffer solution inflow holes, waste liquid outflow holes and connected with the outside world. An electrode is respectively inserted in the sample inflow hole, the buffer solution inflow hole and the waste liquid outflow hole. The laser light emitted by the laser light source is focused on the detection point of the cross channel of the microfluidic chip after passing through the plane mirror and the convex lens. The fluorescence emitted by the substance in the channel is received by the optical fiber under the chip, and then transmitted to the spectroscopic detection system. Said spectroscopic detection system refers to a CCD spectral detector with a volume of only a few cubic centimeters, which is internally composed of a collimating light-parallel concave mirror, a spectroscopic grating and an array detector. The helix is closely connected.

The sample inflow hole, the buffer solution inflow hole, and the waste liquid outflow hole on the microfluidic chip are all bonded with corresponding liquid reservoirs, which are used to hold samples, buffer solution, and waste liquid respectively. Each liquid reservoir communicates with the channel through the sample inflow hole, the buffer solution inflow hole and the waste liquid outflow hole respectively. Electrodes are inserted in each reservoir, and they can be made of platinum wire, gold wire, platinum sheet or gold sheet, but considering stability, electrical conductivity and cost, it is most suitable to choose platinum wire as the electrode. The size of the microfluidic chip is 1-15cm ² , but in order to ensure a certain size of electric field strength, the chip does not need to be too large, preferably 1-6cm ² ; the length of each microchannel is 1-5cm, and the length of each microchannel is 0.5-1.5cm It is the best; the width of each microchannel is 10-150 μm, and 20-100 μm is the best. The material of the microfluidic chip can be monocrystalline silicon, amorphous silicon, glass, quartz, epoxy resin, polyurea, polyurethane, polystyrene, polymethyl methacrylate and dimethyl siloxane. Microfluidic chips of polymer materials with low price and large aspect ratio can be obtained by chemical etching, excimer laser etching, plastic film method and hot pressing method. The microfluidic chip can be used repeatedly or once.

The laser light source can be a blue or green semiconductor laser or an argon ion laser, but because the semiconductor laser has the advantages of small size, no need for water cooling, short warm-up time, and low price, and the blue laser can excite a wide range of fluorescence, especially For quantum dots, quantum dots of various emission wavelengths can be excited by blue lasers, so blue semiconductor lasers are the best.

The technical scheme adopted in the method for detecting biochemical components of the present invention is: using the luminescent quantum dot coded microsphere as a carrier, and first activating the hydroxyl group on the surface of the microsphere. Then, the encoded microspheres are coupled with the capture antibodies, that is, nucleic acids, antibodies or antigens, peptides or cytokines are adsorbed on different encoded luminescent quantum dot encoded microspheres to make labeled microspheres. Then mix it with the sample to be tested, and through specific interactions such as DNA hybridization reaction and immune reaction, all the specific nucleic acids, antibodies or antigens, peptides or cytokines in various samples to be tested are also adsorbed to the corresponding Luminescent quantum dots are encoded on microspheres. Finally, a microfluidic chip flow biochemical analyzer was used to detect and analyze the content of related factors in the sample to be tested.

The luminescent quantum dot coded microspheres have uniform size and contain polystyrene or latex polymer microspheres encoded by various specific proportions of different types of luminescent quantum dots. The invention adopts microspheres with a diameter range of 1-20 μm and emission bandwidth of quantum dots less than 20 nm. The diameter range of the microspheres is 2-6 μm, and the diameter deviation is less than ±5%.

The so-called coupling of encoded microspheres and capture antibodies is to modify the encoded microspheres, and immobilize biological and chemical substances such as nucleic acids, antibodies or antigens, peptides, cytokines, etc. on the encoded microspheres.

The so-called mixing with the sample to be tested means that the microsphere-labeled probe is combined with the corresponding component to be tested in the sample through DNA hybridization reaction, immune reaction and molecular recognition.

In the method of detecting biochemical components, the voltage applied to the sample liquid storage tank is 600-1800V, and the buffer liquid storage tank is 200-1500V. When the voltage increases, the flow speed will increase and the detection time can be shortened. However, due to electrolysis and Joule heat, etc. Influenced by various factors, the optimal voltage is 1000-1500V for the sample cell and 600-1200V for the buffer pool, and the operating voltage can be controlled by the system operating software. The number of microspheres detected per second is 2-500. Due to the limitation of the corresponding speed of the detector, the processing speed of the computer and the detection time, it is advisable to detect 30-200 microspheres per second. The sample volume required for the test is 0.04-0.5mL, and the optimal volume is 0.05-0.1mL.

In the method of detecting biochemical components, the flow injection sampling technology can be used, and the computer can be used to control the flow injection system, so that a certain volume of samples and reagents can be directly introduced, and the operation is more convenient and accurate.

The instrument has a special supporting software, using VC or VB programming, can classify and count different types of coded microspheres according to their fluorescence wavelengths and corresponding intensities, and can simultaneously and intuitively display the number of various coded microspheres displayed on the screen.

The micro-flow biochemical analyzer of the present invention integrates sample introduction, liquid flow control, waste discharge, and spectroscopic detection, has small volume, light weight, and compact structure, can simultaneously obtain fluorescence intensity values of multiple wavelengths, and has a fast response speed , up to milliseconds, can record the full-wavelength spectrum of each fast-flowing microsphere, so that it can detect up to millions of analytes at the same time. The microfluidic chip can be used for automatic operation, and it can be continuously injected and discharged without cleaning, flow injection, and the injection volume of each injection is μL level, which can be precisely controlled. The use of microchip system and electrokinetic focusing technology can reduce the volume of the instrument, and the use of CCD spectral detector as the detection system makes the structure of the instrument simpler, and can detect the light intensity on all wavelengths, and obtain each The advantage of this is that the selection of fluorescent reagents is not limited by its type and emission wavelength, which increases flexibility, thereby expanding the application range, improving flexibility, and making instrument operation easier and reducing costs. Due to the reduction of unstable factors, the overall performance of the instrument is also more stable. In addition to the functions of traditional flow cytometry, the instrument also adds molecular recognition, so it can be widely used in biochemical substance analysis, chemical composition analysis, drug screening, clinical diagnosis and other fields.

The method for detecting biochemical components of the present invention also uses flow injection sampling technology for micro-flow biochemical analyzers, which will greatly increase the analysis speed while reducing the volume and weight of the instrument; adopt self-developed and processed microchips, samples The consumption of reagents is low, and the detection cost is low, which greatly reduces the detection cost; the liquid flow control of the instrument adopts electrokinetic focusing technology, so that the microspheres form a uniform laminar single-row flow surrounded by the buffer, and each microsphere is one by one. By passing through the detector independently, a full spectrum of each encoded microsphere can be obtained, and cumbersome steps such as using a large amount of sheath fluid to introduce the sample can be omitted. The advantage of the present invention is that the content of multiple nucleic acids, antigens or antibodies in the same sample can be obtained at the same time in one test, so as to be used for the diagnosis, differential diagnosis or diagnosis of various related diseases such as tumors, various inflammations and autoimmune diseases Early detection of diseases and drug screening, etc.

Description of drawings

Fig. 1 is a structural schematic diagram of a flow cytometer in the background technology.

Fig. 2 is a structural principle diagram of the microfluidic chip flow biochemical analyzer of the present invention.

Fig. 3 is an appearance diagram of the microfluidic chip.

Figure 4 is a schematic diagram of the flow of solutions and microspheres in the microchannel.

Detailed ways

Embodiment 1 further illustrates structure and working process of the present invention in conjunction with accompanying drawing

In the accompanying drawings, 1 is a laser light source, preferably a blue semiconductor laser; 12 is a plane mirror; 13 is a convex lens; 14 is a microfluidic chip, in which there are two cross channels with a width of several microns to more than one hundred microns 21. At the four end points of the microchannel 21, there are sample inflow holes 17, two opposite buffer solution inflow holes 18, and waste liquid outflow holes connected to the outside world. Sample inflow holes 17, buffer solution inflow holes 18, and waste liquid outflow Corresponding liquid reservoirs are respectively bonded on the holes 20, and an electrode is inserted in each of them; 24 is a detection area, which is located on the channel 21 leading to the waste liquid outflow hole 20; The detection point 24 is opposite, and the other end is closely connected with the detection system 16; the detection system 16 is a CCD spectrum detector.

The microfluidic chip 14 can be composed of two pieces of glass or other materials with a size of several square centimeters to tens of square centimeters bonded together, and one of them is engraved with two cross-shaped channels with a width of several micrometers to more than one hundred micrometers. 21 , the cross channel 21 is divided into four sections, which are respectively a sample channel 22 , a two-section buffer channel 23 and a waste liquid channel 25 . There is a small hole with a diameter of 1-2mm at the end of each channel to connect the microchannel with the outside world. A liquid reservoir can be bonded to each of the small holes. They are respectively the sample liquid reservoir 27 for holding samples, The two buffer liquid storage tanks 28 are used to hold buffer solution, and the waste liquid storage tank 19 is used to hold waste liquid. The electrodes can be four sections of platinum wire inserted in the four liquid storage tanks respectively, so that an electric field of a corresponding magnitude will be generated in the channel 21 after a certain high voltage is applied, and the sample and buffer solution will flow into the waste liquid storage tank 19 at the same time under the action of the electric field.

The microfluidic chip 14 can be placed on a three-dimensional adjustment frame, and clamped by a plastic clip whose width can be adjusted left and right to keep it fixed. The position of the microfluidic chip 14 can be adjusted by the knob outside the instrument. To ensure that the laser light is just focused on the detection point 24, it is easy to use and the accuracy can reach micron level.

When detecting a sample, the laser beam emitted by the laser light source 1 passes through the plane mirror 12 and the convex lens 13 and then focuses on the detection point 24 about 50 μm downstream of the intersection point of the microfluidic chip 14 . The coded microspheres flow in a laminar flow surrounded by the buffer, and can pass through the intersection of the channel 21 in a single row. After being excited by the laser, each individual microsphere emits a variety of fluorescent signals with different wavelengths, which can be detected by the optical fiber 15. It is collected at one end and introduced into a detection system 16 by an optical fiber 15 for full-wavelength detection and analysis.

After the light intensity signal obtained above is converted into a digital signal that can be recognized by the computer, the difference between the fluorescence wavelength and intensity on each microsphere is distinguished by software, and each code is counted separately, and finally the detection conclusion is drawn according to the data characteristics .

The voltage applied to the electrodes can also be uniformly controlled by a computer. The software uses VC or VB language for programming, and the number of each type of coded microspheres can be intuitively displayed on the screen.

Both sample introduction and waste liquid extraction can be completed by the flow injection system, which can realize continuous measurement of various samples without cleaning the microfluidic chip 14 .

The detection system 16 can also use a plurality of photomultiplier tubes as a detector, and adopt a plurality of dichroic filters to separate various monochromatic lights, thereby detecting the intensity of fluorescence of various wavelengths on each microsphere. Although this method responds The speed is faster and the sensitivity is higher, but the device is quite complicated and the volume is much larger.

Example 2 Antigen contained in detection sample:

1. Shake the coded microspheres in a vortex shaker and an ultrasonic shaker to suspend them evenly; 3-(3-Dimethylaminopropyl) carbodiimide salt (EDC) was mixed and shaken, and left at room temperature for 20 minutes to activate the carboxyl groups on the surface of the microspheres.

2. The activated microspheres were washed 3 times with PBS solution, then suspended by vortex, and the capture antibody was added quickly, after mixing, shake and incubate at room temperature for 30min, centrifuge at 200g for 15min, and discard the supernatant.

3. Suspend the microspheres in BSA containing 1mg/mL and 0.02% Tween 20, incubate at 4°C for 30min, centrifuge at 200g for 15min, discard the supernatant; wash the microspheres twice with the above buffer, and suspend them in Incubate in 1mL of cell culture medium at 4°C for 20min; count with a hemocytometer, adjust the concentration of suspended microspheres to 2×10 ⁶ /mL, and place in a dark place at 4°C;

4. Mix the sample to be tested with microspheres linked with various specific antibodies in a small test tube, suspend and shake, incubate at 4°C for 30 minutes; centrifuge at 200g for 15 minutes, and discard the supernatant.

5. Add anti-species-specific IgG antibodies labeled with quantum dots or other colored organic dyes, incubate at 4°C for 45 minutes; centrifuge at 200g for 15 minutes, and discard the supernatant. Wash twice with PBS to remove excess detection antibody, and suspend in 50 μL of PBS-FTN solution; put the above-mentioned treated sample into the sample pool above the chip in the micro-flow biochemical analyzer for analysis and detection.

6. Negative control group setting: set a non-specific control group for each sample, replace the specific antibody with PBS, and the rest of the operations are the same as above.

Example 3 Detection of cytokines by microsphere-labeled receptors

1. The process of activating the hydroxyl groups on the surface of the microspheres is the same as in Example 2.

2. Add receptors such as IL-1, 2, 6, and 12 to each microsphere, each microsphere corresponds to a receptor, mix and incubate at room temperature for 30 minutes, centrifuge at 200g for 15 minutes, and discard the supernatant ;

3. Suspend the microspheres in BSA containing 1mg/mL and 0.02% Tween 20, incubate at 4°C for 30min, centrifuge at 200g for 15min, discard the supernatant; wash the microspheres twice with the above buffer, and suspend them in Incubate in 1mL of cell culture medium at 4°C for 20min; count with a hemocytometer, adjust the concentration of suspended microspheres to 2×10 ⁶ /mL, and place in a dark place at 4°C;

4. Add 100 μL of the solution containing the cytokine to be tested, incubate at 4°C for 45 minutes; centrifuge at 200 g for 15 minutes, and discard the supernatant.

5. Add another series of colors of quantum dots or fluorescent dye-labeled IL-1, 2, 6, 12 and other antibodies, mix with microspheres connected with various receptors in a small test tube, vortex, and incubate at 4°C for 45 minutes Centrifuge at 200g for 15min, discard the supernatant; wash twice with PBS to remove excess detection antibody, add 50μL PBS (containing 0.1% sodium azide) buffer, and analyze on the machine. The percentage content of each cytokine was determined.

6. Setting of negative control group: same as in Example 2.

Example 4 Microsphere-labeled oligonucleotide probes for gene detection

1. The process of activating the hydroxyl groups on the surface of the microspheres is the same as in Example 2.

2. Add a coded microsphere and one of the oligonucleotide fragments F1, F2, F6, and F12 of IL-1, 2, 6, and 12 genes to each test tube, each microsphere corresponds to a Oligonucleotide fragments; after mixing, incubate at room temperature in the dark for 15min-30min, centrifuge at 200g for 15min, and discard the supernatant.

3. Suspend the microspheres in BSA containing 1mg/mL and 0.02% Tween 20, incubate at 4°C for 30min, centrifuge at 200g for 15min, discard the supernatant; wash the microspheres twice with the above buffer, and suspend them in Incubate in 1mL of cell culture medium at 4°C for 20min; count with a hemocytometer, adjust the concentration of suspended microspheres to 2×10 ⁶ /mL, and place in a dark place at 4°C;

4. Add 10 μL of the sample to be tested, incubate at room temperature in the dark for 30 minutes, centrifuge at 200 g for 15 minutes, and discard the supernatant.

5. Add 5 μL each of the oligonucleotide fragments R1, R2, R6, and R12 of another series of quantum dots or fluorescent dye-labeled IL-1, 2, 6, and 12 genes (F and R sequences are not complementary); mix After uniformity, incubate at room temperature and in the dark for 15min-30min, centrifuge at 200g for 15min, discard the supernatant; wash twice with PBS to remove excess detection antibody, add 50μL PBS (containing 0.1% sodium azide) buffer, and put on Machine analysis to determine the percentage content of each oligonucleotide.

6. Negative control group setting: each sample was set as a non-specific control group, and PBS was used to replace the specific probe, and the rest were the same as above.

Example 5 Microsphere-labeled peptides for detection of specific receptors

1. The process of activating the hydroxyl groups on the surface of the microspheres is the same as in Example 2.

2. Add a coded microsphere and one of IL-1, 2, 6, and 12 peptides F1, F2, F6, and F12 to each test tube, and each microsphere modifies a peptide; after mixing Incubate at room temperature in the dark for 15min-30min, centrifuge at 200g for 15min, and discard the supernatant.

3. Suspend the microspheres in BSA containing 1mg/mL and 0.02% Tween 20, incubate at 4°C for 30min, centrifuge at 200g for 15min, discard the supernatant; wash the microspheres twice with the above buffer, and suspend them in Incubate in 1mL of cell culture medium at 4°C for 20min; count with a hemocytometer, adjust the concentration of suspended microspheres to 2×10 ⁶ /mL, and place in a dark place at 4°C;

4. Add the sample to be tested, incubate at room temperature in the dark for 30 minutes, centrifuge at 200 g for 15 minutes, and discard the supernatant.

5. Add 5 μL each of quantum dots of another series of colors or fluorescent dye-labeled antibodies RAb1, RAb2, RAb6, and RAb12 to IL-1, RAb2, RAb6, and RAb12; mix well and incubate at room temperature in the dark for 15min-30min , centrifuged at 200g for 15min, discarded the supernatant; washed twice with PBS to remove excess detection antibody, added 50μL PBS (containing 0.1% sodium azide) buffer, analyzed on the machine, and determined the percentage content of each peptide .

6. Setting of negative control group: same as in Example 2.

Claims (5)

1, a kind of micro-fluidic chip streaming Biochemical Analyzer, its structure includes LASER Light Source (1), spectral detection system (16), and promptly the CCD spectroscopic detector is characterized in that, and its structure also has micro-fluidic chip (14); Said micro-fluidic chip (14) is two-layer thin plate through etching and bonding and make, be carved with the passage (21) of right-angled intersection between the two-layer thin slice, the destination county of passage (21) is respectively sample flow hand-hole (17), relative two buffer solution ostiums (18), waste liquid tap hole (20); Sample flow hand-hole (17), buffer solution ostium (18), waste liquid tap hole are bonded with corresponding liquid storage tank respectively above (20), and each liquid storage tank communicates with passage (21) by sample flow hand-hole (17), buffer solution ostium (18), waste liquid tap hole (20) respectively; Respectively be fitted with electrode in the liquid storage tank; The laser that LASER Light Source (1) is sent focuses on the check point (24) of the right-angled intersection passage of micro-fluidic chip (14) behind level crossing (12), convex lens (13); The fluorescence that material in the passage (21) sends is received by the optical fiber (15) of micro-fluidic chip (14) below, imports spectral detection system (16) then into.

According to the described micro-fluidic chip streaming of claim 1 Biochemical Analyzer, it is characterized in that 2, the electrode of plug-in mounting is platinum filament or spun gold or platinized platinum or gold plaque in each liquid storage tank.

According to claim 1 or 2 described micro-fluidic chip streaming Biochemical Analyzers, it is characterized in that 3, said LASER Light Source (1) is blue or green semiconductor laser.

4, the application of a kind of micro-fluidic chip streaming Biochemical Analyzer of claim 1 in detecting biochemical component is characterized in that, uses the luminescent quantum dot coding microball to be carrier; At first activate the hydroxyl of microsphere surface; Secondly with coding microball and capture antibodies coupling connection, be about to nucleic acid, antibody or antigen, peptide class or cell factor and be adsorbed on the microballoon of encoding, make the mark microballoon with luminescent quantum dot; Mix with testing sample once more, interact, the specific nucleic acid in the testing sample, antibody or antigen, peptide class or cell factor are adsorbed onto on the corresponding luminescent quantum dot coding microball by specificity; Use the content of correlation factor in the micro-fluidic chip streaming Biochemical Analyzer check and analysis testing sample at last.

5, according to the application of the described micro-fluidic chip streaming of claim 4 Biochemical Analyzer in detecting biochemical component, it is characterized in that, using micro-fluidic chip streaming Biochemical Analyzer to detect in the biochemical component, sample liquid storage tank (27) institute making alive is 600-1800V, and damping fluid liquid storage tank (28) institute making alive is 200-1500V.

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