CN1218510A - 无融合生殖种子的产生方法 - Google Patents

无融合生殖种子的产生方法 Download PDF

Info

Publication number: CN1218510A
Authority: CN; China
Prior art keywords: leu; sequence; dna; asn; pro
Prior art date: 1996-05-14
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

CN97194634A

Other languages

English (en)

Chinese (zh)

Inventor

S·C·德夫里斯

E·D·L·史密特

G·J·范霍尔斯特

V·F·G·赫克特

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Syngenta Participations AG

Original Assignee

Novartis AG

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-05-14

Filing date

1997-05-13

Publication date

1999-06-02

1997-05-13 Application filed by Novartis AG filed Critical Novartis AG

1999-06-02 Publication of CN1218510A publication Critical patent/CN1218510A/zh

Status Pending legal-status Critical Current

Links

238000004519 manufacturing process Methods 0.000 title description 8
108090000623 proteins and genes Proteins 0.000 claims abstract description 190
102000004169 proteins and genes Human genes 0.000 claims abstract description 121
238000000034 method Methods 0.000 claims abstract description 95
210000001161 mammalian embryo Anatomy 0.000 claims abstract description 75
239000000463 material Substances 0.000 claims abstract description 21
239000012528 membrane Substances 0.000 claims abstract description 18
108020001756 ligand binding domains Proteins 0.000 claims abstract description 12
239000002773 nucleotide Substances 0.000 claims abstract description 11
125000003729 nucleotide group Chemical group 0.000 claims abstract description 11
230000000408 embryogenic effect Effects 0.000 claims abstract description 8
125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 claims abstract description 8
210000004027 cell Anatomy 0.000 claims description 174
241000196324 Embryophyta Species 0.000 claims description 153
108020004414 DNA Proteins 0.000 claims description 77
230000014509 gene expression Effects 0.000 claims description 50
108091000080 Phosphotransferase Proteins 0.000 claims description 41
150000001413 amino acids Chemical group 0.000 claims description 41
238000009396 hybridization Methods 0.000 claims description 40
102000020233 phosphotransferase Human genes 0.000 claims description 40
230000000694 effects Effects 0.000 claims description 33
241000219194 Arabidopsis Species 0.000 claims description 23
230000000392 somatic effect Effects 0.000 claims description 22
108020004999 messenger RNA Proteins 0.000 claims description 20
239000005418 vegetable material Substances 0.000 claims description 18
230000002068 genetic effect Effects 0.000 claims description 15
108091028043 Nucleic acid sequence Proteins 0.000 claims description 13
102000001253 Protein Kinase Human genes 0.000 claims description 13
230000035578 autophosphorylation Effects 0.000 claims description 13
230000010152 pollination Effects 0.000 claims description 12
108060006633 protein kinase Proteins 0.000 claims description 11
230000000295 complement effect Effects 0.000 claims description 10
230000001939 inductive effect Effects 0.000 claims description 10
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 9
230000012010 growth Effects 0.000 claims description 9
108020004511 Recombinant DNA Proteins 0.000 claims description 8
235000013311 vegetables Nutrition 0.000 claims description 8
101150031639 IV gene Proteins 0.000 claims description 7
150000001875 compounds Chemical class 0.000 claims description 7
210000001672 ovary Anatomy 0.000 claims description 7
108020004705 Codon Proteins 0.000 claims description 6
101710196809 Non-specific lipid-transfer protein 1 Proteins 0.000 claims description 6
241001505935 Phalaenopsis Species 0.000 claims description 6
230000006698 induction Effects 0.000 claims description 6
230000008488 polyadenylation Effects 0.000 claims description 6
108700030152 Arabidopsis AINTEGUMENTA Proteins 0.000 claims description 5
108700037463 Arabidopsis BEL1 Proteins 0.000 claims description 5
RVMXMLSYBTXCAV-VEVYYDQMSA-N Asp-Pro-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(O)=O RVMXMLSYBTXCAV-VEVYYDQMSA-N 0.000 claims description 5
102000012286 Chitinases Human genes 0.000 claims description 5
108010022172 Chitinases Proteins 0.000 claims description 5
QENSHQJGWGRPQS-QEJZJMRPSA-N Gln-Ser-Trp Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)N)C(O)=O)=CNC2=C1 QENSHQJGWGRPQS-QEJZJMRPSA-N 0.000 claims description 5
230000004927 fusion Effects 0.000 claims description 5
230000004048 modification Effects 0.000 claims description 5
238000012986 modification Methods 0.000 claims description 5
108700035929 Arabidopsis ATDMC1 Proteins 0.000 claims description 4
108700026244 Open Reading Frames Proteins 0.000 claims description 4
210000000170 cell membrane Anatomy 0.000 claims description 4
FANFRJOFTYCNRG-JYBASQMISA-N Cys-Thr-Trp Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)O)NC(=O)[C@H](CS)N)O FANFRJOFTYCNRG-JYBASQMISA-N 0.000 claims description 3
RGUXWMDNCPMQFB-YUMQZZPRSA-N Leu-Ser-Gly Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)NCC(O)=O RGUXWMDNCPMQFB-YUMQZZPRSA-N 0.000 claims description 3
XZNJZXJZBMBGGS-NHCYSSNCSA-N Leu-Val-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O XZNJZXJZBMBGGS-NHCYSSNCSA-N 0.000 claims description 3
SPXWRYVHOZVYBU-ULQDDVLXSA-N Phe-His-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CN=CN1)NC(=O)[C@H](CC2=CC=CC=C2)N SPXWRYVHOZVYBU-ULQDDVLXSA-N 0.000 claims description 3
ODSAPYVQSLDRSR-LKXGYXEUSA-N Thr-Cys-Asn Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(O)=O ODSAPYVQSLDRSR-LKXGYXEUSA-N 0.000 claims description 3
238000000338 in vitro Methods 0.000 claims description 3
238000002156 mixing Methods 0.000 claims description 3
230000002018 overexpression Effects 0.000 claims description 3
230000008929 regeneration Effects 0.000 claims description 3
238000011069 regeneration method Methods 0.000 claims description 3
230000001105 regulatory effect Effects 0.000 claims description 3
QTAIIXQCOPUNBQ-QXEWZRGKSA-N Arg-Val-Asp Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O QTAIIXQCOPUNBQ-QXEWZRGKSA-N 0.000 claims description 2
FMEICTQWUKNAGC-YUMQZZPRSA-N Leu-Gly-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(O)=O FMEICTQWUKNAGC-YUMQZZPRSA-N 0.000 claims description 2
NXEYSLRNNPWCRN-SRVKXCTJSA-N Pro-Glu-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O NXEYSLRNNPWCRN-SRVKXCTJSA-N 0.000 claims description 2
IDKGBVZGNTYYCC-QXEWZRGKSA-N Val-Asn-Pro Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N1CCC[C@H]1C(O)=O IDKGBVZGNTYYCC-QXEWZRGKSA-N 0.000 claims description 2
239000003375 plant hormone Substances 0.000 claims description 2
241000207748 Petunia Species 0.000 claims 2
XJROSHJRQTXWAE-XGEHTFHBSA-N Pro-Cys-Thr Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)O)C(O)=O XJROSHJRQTXWAE-XGEHTFHBSA-N 0.000 claims 2
VUMCLPHXCBIJJB-PMVMPFDFSA-N Trp-Phe-His Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CC2=CN=CN2)C(=O)O)NC(=O)[C@H](CC3=CNC4=CC=CC=C43)N VUMCLPHXCBIJJB-PMVMPFDFSA-N 0.000 claims 2
BZDGLJPROOOUOZ-XGEHTFHBSA-N Val-Thr-Cys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](C(C)C)N)O BZDGLJPROOOUOZ-XGEHTFHBSA-N 0.000 claims 2
230000008034 disappearance Effects 0.000 claims 1
108010020688 glycylhistidine Proteins 0.000 claims 1
230000002779 inactivation Effects 0.000 claims 1
108091005682 Receptor kinases Proteins 0.000 abstract description 9
230000001131 transforming effect Effects 0.000 abstract description 7
230000001172 regenerating effect Effects 0.000 abstract description 2
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 670
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 306
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 303
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 295
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 262
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 237
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 236
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 229
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 152
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 145
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 113
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 106
235000018102 proteins Nutrition 0.000 description 88
239000002299 complementary DNA Substances 0.000 description 49
239000002609 medium Substances 0.000 description 37
235000001014 amino acid Nutrition 0.000 description 30
229940024606 amino acid Drugs 0.000 description 29
239000012634 fragment Substances 0.000 description 29
238000006243 chemical reaction Methods 0.000 description 28
102220023256 rs387907547 Human genes 0.000 description 27
239000000523 sample Substances 0.000 description 26
108020004707 nucleic acids Proteins 0.000 description 24
102000039446 nucleic acids Human genes 0.000 description 24
150000007523 nucleic acids Chemical class 0.000 description 24
238000013016 damping Methods 0.000 description 23
239000012530 fluid Substances 0.000 description 23
239000000047 product Substances 0.000 description 21
210000001519 tissue Anatomy 0.000 description 21
239000013598 vector Substances 0.000 description 21
230000000692 anti-sense effect Effects 0.000 description 20
102220023257 rs387907546 Human genes 0.000 description 18
244000000626 Daucus carota Species 0.000 description 17
235000002767 Daucus carota Nutrition 0.000 description 17
102220369445 c.668T>C Human genes 0.000 description 16
230000009261 transgenic effect Effects 0.000 description 16
108091032973 (ribonucleotides)n+m Proteins 0.000 description 15
239000000499 gel Substances 0.000 description 15
230000008569 process Effects 0.000 description 15
238000012216 screening Methods 0.000 description 15
108060001084 Luciferase Proteins 0.000 description 13
230000009182 swimming Effects 0.000 description 13
238000012360 testing method Methods 0.000 description 13
238000005406 washing Methods 0.000 description 13
241000219195 Arabidopsis thaliana Species 0.000 description 12
238000009395 breeding Methods 0.000 description 12
230000001488 breeding effect Effects 0.000 description 12
238000007901 in situ hybridization Methods 0.000 description 12
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 11
230000004913 activation Effects 0.000 description 11
238000004114 suspension culture Methods 0.000 description 11
235000002017 Zea mays subsp mays Nutrition 0.000 description 10
238000004113 cell culture Methods 0.000 description 10
241000894006 Bacteria Species 0.000 description 9
206010020649 Hyperkeratosis Diseases 0.000 description 9
239000005089 Luciferase Substances 0.000 description 9
241000209149 Zea Species 0.000 description 9
241000589158 Agrobacterium Species 0.000 description 8
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 8
238000004458 analytical method Methods 0.000 description 8
230000001580 bacterial effect Effects 0.000 description 8
235000005822 corn Nutrition 0.000 description 8
238000005516 engineering process Methods 0.000 description 8
230000008595 infiltration Effects 0.000 description 8
238000001764 infiltration Methods 0.000 description 8
239000010410 layer Substances 0.000 description 8
239000000203 mixture Substances 0.000 description 8
239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 7
241000238631 Hexapoda Species 0.000 description 7
241000700605 Viruses Species 0.000 description 7
238000002474 experimental method Methods 0.000 description 7
238000011534 incubation Methods 0.000 description 7
238000003757 reverse transcription PCR Methods 0.000 description 7
239000000725 suspension Substances 0.000 description 7
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 6
IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 6
CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 6
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 6
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 6
DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 6
240000007377 Petunia x hybrida Species 0.000 description 6
239000000284 extract Substances 0.000 description 6
239000013612 plasmid Substances 0.000 description 6
230000001568 sexual effect Effects 0.000 description 6
239000011780 sodium chloride Substances 0.000 description 6
238000012546 transfer Methods 0.000 description 6
229920000936 Agarose Polymers 0.000 description 5
102000004190 Enzymes Human genes 0.000 description 5
108090000790 Enzymes Proteins 0.000 description 5
MTAOBYXRYJZRGQ-WDSKDSINSA-N Glu-Gly-Asp Chemical compound OC(=O)CC[C@H](N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O MTAOBYXRYJZRGQ-WDSKDSINSA-N 0.000 description 5
235000010469 Glycine max Nutrition 0.000 description 5
244000068988 Glycine max Species 0.000 description 5
240000007594 Oryza sativa Species 0.000 description 5
235000007164 Oryza sativa Nutrition 0.000 description 5
BJEYSVHMGIJORT-NHCYSSNCSA-N Phe-Ala-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC1=CC=CC=C1 BJEYSVHMGIJORT-NHCYSSNCSA-N 0.000 description 5
230000003321 amplification Effects 0.000 description 5
238000000137 annealing Methods 0.000 description 5
102220369447 c.1352G>A Human genes 0.000 description 5
230000008859 change Effects 0.000 description 5
230000029087 digestion Effects 0.000 description 5
210000002615 epidermis Anatomy 0.000 description 5
108010025306 histidylleucine Proteins 0.000 description 5
239000005556 hormone Substances 0.000 description 5
229940088597 hormone Drugs 0.000 description 5
108010044056 leucyl-phenylalanine Proteins 0.000 description 5
238000003199 nucleic acid amplification method Methods 0.000 description 5
235000009566 rice Nutrition 0.000 description 5
239000002356 single layer Substances 0.000 description 5
239000000243 solution Substances 0.000 description 5
238000011282 treatment Methods 0.000 description 5
241000701447 unidentified baculovirus Species 0.000 description 5
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
FXKNPWNXPQZLES-ZLUOBGJFSA-N Ala-Asn-Ser Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(O)=O FXKNPWNXPQZLES-ZLUOBGJFSA-N 0.000 description 4
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 4
229930182566 Gentamicin Natural products 0.000 description 4
JFSGIJSCJFQGSZ-MXAVVETBSA-N Leu-Ile-His Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)NC(=O)[C@H](CC(C)C)N JFSGIJSCJFQGSZ-MXAVVETBSA-N 0.000 description 4
AIMGJYMCTAABEN-GVXVVHGQSA-N Leu-Val-Glu Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O AIMGJYMCTAABEN-GVXVVHGQSA-N 0.000 description 4
YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 description 4
235000002637 Nicotiana tabacum Nutrition 0.000 description 4
244000061176 Nicotiana tabacum Species 0.000 description 4
240000004713 Pisum sativum Species 0.000 description 4
235000010582 Pisum sativum Nutrition 0.000 description 4
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 4
108010093581 aspartyl-proline Proteins 0.000 description 4
230000015572 biosynthetic process Effects 0.000 description 4
239000000872 buffer Substances 0.000 description 4
230000001413 cellular effect Effects 0.000 description 4
230000004087 circulation Effects 0.000 description 4
238000010367 cloning Methods 0.000 description 4
238000012258 culturing Methods 0.000 description 4
238000006062 fragmentation reaction Methods 0.000 description 4
229960002518 gentamicin Drugs 0.000 description 4
108010049041 glutamylalanine Proteins 0.000 description 4
RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 4
238000005286 illumination Methods 0.000 description 4
230000001965 increasing effect Effects 0.000 description 4
230000003993 interaction Effects 0.000 description 4
238000002955 isolation Methods 0.000 description 4
KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 4
239000006870 ms-medium Substances 0.000 description 4
210000000056 organ Anatomy 0.000 description 4
108090000765 processed proteins & peptides Proteins 0.000 description 4
230000001850 reproductive effect Effects 0.000 description 4
HJYYPODYNSCCOU-ODRIEIDWSA-N rifamycin SV Chemical compound OC1=C(C(O)=C2C)C3=C(O)C=C1NC(=O)\C(C)=C/C=C/[C@H](C)[C@H](O)[C@@H](C)[C@@H](O)[C@@H](C)[C@H](OC(C)=O)[C@H](C)[C@@H](OC)\C=C\O[C@@]1(C)OC2=C3C1=O HJYYPODYNSCCOU-ODRIEIDWSA-N 0.000 description 4
102220023258 rs387907548 Human genes 0.000 description 4
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 4
239000002689 soil Substances 0.000 description 4
238000001890 transfection Methods 0.000 description 4
230000009466 transformation Effects 0.000 description 4
230000010474 transient expression Effects 0.000 description 4
230000002792 vascular Effects 0.000 description 4
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
101100421609 Arabidopsis thaliana SKIP32 gene Proteins 0.000 description 3
OTUQSEPIIVBYEM-IHRRRGAJSA-N Arg-Asn-Tyr Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O OTUQSEPIIVBYEM-IHRRRGAJSA-N 0.000 description 3
XOZYYXMHMIEJET-XIRDDKMYSA-N Arg-Trp-Glu Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CCC(O)=O)C(O)=O XOZYYXMHMIEJET-XIRDDKMYSA-N 0.000 description 3
244000075850 Avena orientalis Species 0.000 description 3
241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 3
235000006008 Brassica napus var napus Nutrition 0.000 description 3
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 3
241000209510 Liliopsida Species 0.000 description 3
235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
WXHHTBVYQOSYSL-FXQIFTODSA-N Met-Ala-Ser Chemical compound CSCC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O WXHHTBVYQOSYSL-FXQIFTODSA-N 0.000 description 3
108091034057 RNA (poly(A)) Proteins 0.000 description 3
101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 3
241000209056 Secale Species 0.000 description 3
240000003768 Solanum lycopersicum Species 0.000 description 3
235000021536 Sugar beet Nutrition 0.000 description 3
BVOVIGCHYNFJBZ-JXUBOQSCSA-N Thr-Leu-Ala Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O BVOVIGCHYNFJBZ-JXUBOQSCSA-N 0.000 description 3
-1 Threonine phosphoric acid ester Chemical class 0.000 description 3
241000209140 Triticum Species 0.000 description 3
235000021307 Triticum Nutrition 0.000 description 3
239000002671 adjuvant Substances 0.000 description 3
230000008901 benefit Effects 0.000 description 3
102220369446 c.1274G>A Human genes 0.000 description 3
230000006378 damage Effects 0.000 description 3
238000011161 development Methods 0.000 description 3
230000018109 developmental process Effects 0.000 description 3
238000006471 dimerization reaction Methods 0.000 description 3
238000004043 dyeing Methods 0.000 description 3
235000013399 edible fruits Nutrition 0.000 description 3
238000004520 electroporation Methods 0.000 description 3
230000013020 embryo development Effects 0.000 description 3
210000002257 embryonic structure Anatomy 0.000 description 3
239000013604 expression vector Substances 0.000 description 3
238000011049 filling Methods 0.000 description 3
238000013467 fragmentation Methods 0.000 description 3
210000001822 immobilized cell Anatomy 0.000 description 3
238000001727 in vivo Methods 0.000 description 3
208000015181 infectious disease Diseases 0.000 description 3
230000005764 inhibitory process Effects 0.000 description 3
230000003834 intracellular effect Effects 0.000 description 3
XUWPJKDMEZSVTP-LTYMHZPRSA-N kalafungina Chemical compound O=C1C2=C(O)C=CC=C2C(=O)C2=C1[C@@H](C)O[C@H]1[C@@H]2OC(=O)C1 XUWPJKDMEZSVTP-LTYMHZPRSA-N 0.000 description 3
230000035800 maturation Effects 0.000 description 3
230000001404 mediated effect Effects 0.000 description 3
230000000442 meristematic effect Effects 0.000 description 3
108010016686 methionyl-alanyl-serine Proteins 0.000 description 3
230000037039 plant physiology Effects 0.000 description 3
230000001681 protective effect Effects 0.000 description 3
230000007226 seed germination Effects 0.000 description 3
238000000926 separation method Methods 0.000 description 3
210000002966 serum Anatomy 0.000 description 3
238000009331 sowing Methods 0.000 description 3
241000894007 species Species 0.000 description 3
238000004659 sterilization and disinfection Methods 0.000 description 3
238000006467 substitution reaction Methods 0.000 description 3
230000002103 transcriptional effect Effects 0.000 description 3
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
QRXMUCSWCMTJGU-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-N 0.000 description 2
244000291564 Allium cepa Species 0.000 description 2
235000002732 Allium cepa var. cepa Nutrition 0.000 description 2
101000949831 Arabidopsis thaliana Meiotic recombination protein DMC1 homolog Proteins 0.000 description 2
WKPXXXUSUHAXDE-SRVKXCTJSA-N Arg-Pro-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C(N)N)C(O)=O WKPXXXUSUHAXDE-SRVKXCTJSA-N 0.000 description 2
AWMAZIIEFPFHCP-RCWTZXSCSA-N Arg-Pro-Thr Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(O)=O AWMAZIIEFPFHCP-RCWTZXSCSA-N 0.000 description 2
AITGTTNYKAWKDR-CIUDSAMLSA-N Asn-His-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(O)=O AITGTTNYKAWKDR-CIUDSAMLSA-N 0.000 description 2
QIRJQYQOIKBPBZ-IHRRRGAJSA-N Asn-Tyr-Arg Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O QIRJQYQOIKBPBZ-IHRRRGAJSA-N 0.000 description 2
241000201370 Autographa californica nucleopolyhedrovirus Species 0.000 description 2
240000002791 Brassica napus Species 0.000 description 2
235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
244000025254 Cannabis sativa Species 0.000 description 2
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
235000007516 Chrysanthemum Nutrition 0.000 description 2
244000189548 Chrysanthemum x morifolium Species 0.000 description 2
229920000742 Cotton Polymers 0.000 description 2
102000004594 DNA Polymerase I Human genes 0.000 description 2
108010017826 DNA Polymerase I Proteins 0.000 description 2
238000001712 DNA sequencing Methods 0.000 description 2
241000208175 Daucus Species 0.000 description 2
101150048726 E9 gene Proteins 0.000 description 2
108010067770 Endopeptidase K Proteins 0.000 description 2
241000588724 Escherichia coli Species 0.000 description 2
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
108010024636 Glutathione Proteins 0.000 description 2
KQDMENMTYNBWMR-WHFBIAKZSA-N Gly-Asp-Ala Chemical compound [H]NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(O)=O KQDMENMTYNBWMR-WHFBIAKZSA-N 0.000 description 2
229920002527 Glycogen Polymers 0.000 description 2
244000299507 Gossypium hirsutum Species 0.000 description 2
235000003222 Helianthus annuus Nutrition 0.000 description 2
244000020551 Helianthus annuus Species 0.000 description 2
HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
IZPVWNSAVUQBGP-CIUDSAMLSA-N Leu-Ser-Asp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O IZPVWNSAVUQBGP-CIUDSAMLSA-N 0.000 description 2
XOWMDXHFSBCAKQ-SRVKXCTJSA-N Leu-Ser-Leu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CC(C)C XOWMDXHFSBCAKQ-SRVKXCTJSA-N 0.000 description 2
FPFOYSCDUWTZBF-IHPCNDPISA-N Leu-Trp-Leu Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H]([NH3+])CC(C)C)C(=O)N[C@@H](CC(C)C)C([O-])=O)=CNC2=C1 FPFOYSCDUWTZBF-IHPCNDPISA-N 0.000 description 2
HLFSDGLLUJUHTE-SNVBAGLBSA-N Levamisole Chemical compound C1([C@H]2CN3CCSC3=N2)=CC=CC=C1 HLFSDGLLUJUHTE-SNVBAGLBSA-N 0.000 description 2
HLQWFLJOJRFXHO-CIUDSAMLSA-N Met-Glu-Ser Chemical compound [H]N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O HLQWFLJOJRFXHO-CIUDSAMLSA-N 0.000 description 2
239000004677 Nylon Substances 0.000 description 2
108091034117 Oligonucleotide Proteins 0.000 description 2
229930040373 Paraformaldehyde Natural products 0.000 description 2
DVOCGBNHAUHKHJ-DKIMLUQUSA-N Phe-Ile-Leu Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(O)=O DVOCGBNHAUHKHJ-DKIMLUQUSA-N 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
CJZTUKSFZUSNCC-FXQIFTODSA-N Pro-Asp-Asn Chemical compound NC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H]1CCCN1 CJZTUKSFZUSNCC-FXQIFTODSA-N 0.000 description 2
FDMCIBSQRKFSTJ-RHYQMDGZSA-N Pro-Thr-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(O)=O FDMCIBSQRKFSTJ-RHYQMDGZSA-N 0.000 description 2
108010009341 Protein Serine-Threonine Kinases Proteins 0.000 description 2
102000009516 Protein Serine-Threonine Kinases Human genes 0.000 description 2
238000012300 Sequence Analysis Methods 0.000 description 2
MUJQWSAWLLRJCE-KATARQTJSA-N Ser-Leu-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O MUJQWSAWLLRJCE-KATARQTJSA-N 0.000 description 2
UQGAAZXSCGWMFU-UBHSHLNASA-N Ser-Trp-Asp Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)[C@H](CO)N UQGAAZXSCGWMFU-UBHSHLNASA-N 0.000 description 2
244000046109 Sorghum vulgare var. nervosum Species 0.000 description 2
241000256251 Spodoptera frugiperda Species 0.000 description 2
229930006000 Sucrose Natural products 0.000 description 2
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
239000004473 Threonine Substances 0.000 description 2
241000219793 Trifolium Species 0.000 description 2
YXONONCLMLHWJX-SZMVWBNQSA-N Trp-Glu-Leu Chemical compound C1=CC=C2C(C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O)=CNC2=C1 YXONONCLMLHWJX-SZMVWBNQSA-N 0.000 description 2
VVIZITNVZUAEMI-DLOVCJGASA-N Val-Val-Gln Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](C(O)=O)CCC(N)=O VVIZITNVZUAEMI-DLOVCJGASA-N 0.000 description 2
108010059993 Vancomycin Proteins 0.000 description 2
235000010726 Vigna sinensis Nutrition 0.000 description 2
244000042314 Vigna unguiculata Species 0.000 description 2
241000482268 Zea mays subsp. mays Species 0.000 description 2
HMNZFMSWFCAGGW-XPWSMXQVSA-N [3-[hydroxy(2-hydroxyethoxy)phosphoryl]oxy-2-[(e)-octadec-9-enoyl]oxypropyl] (e)-octadec-9-enoate Chemical compound CCCCCCCC\C=C\CCCCCCCC(=O)OCC(COP(O)(=O)OCCO)OC(=O)CCCCCCC\C=C\CCCCCCCC HMNZFMSWFCAGGW-XPWSMXQVSA-N 0.000 description 2
230000003213 activating effect Effects 0.000 description 2
125000000539 amino acid group Chemical group 0.000 description 2
239000000074 antisense oligonucleotide Substances 0.000 description 2
238000012230 antisense oligonucleotides Methods 0.000 description 2
238000013459 approach Methods 0.000 description 2
239000011324 bead Substances 0.000 description 2
RTYJTGSCYUUYAL-YCAHSCEMSA-L carbenicillin disodium Chemical compound [Na+].[Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)C(C([O-])=O)C1=CC=CC=C1 RTYJTGSCYUUYAL-YCAHSCEMSA-L 0.000 description 2
210000002421 cell wall Anatomy 0.000 description 2
239000001913 cellulose Substances 0.000 description 2
229920002678 cellulose Polymers 0.000 description 2
238000010276 construction Methods 0.000 description 2
230000001086 cytosolic effect Effects 0.000 description 2
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
239000002274 desiccant Substances 0.000 description 2
XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
201000010099 disease Diseases 0.000 description 2
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
241001233957 eudicotyledons Species 0.000 description 2
230000004720 fertilization Effects 0.000 description 2
238000011010 flushing procedure Methods 0.000 description 2
229960003180 glutathione Drugs 0.000 description 2
229940096919 glycogen Drugs 0.000 description 2
108010050848 glycylleucine Proteins 0.000 description 2
239000008187 granular material Substances 0.000 description 2
229960002897 heparin Drugs 0.000 description 2
229920000669 heparin Polymers 0.000 description 2
230000007062 hydrolysis Effects 0.000 description 2
238000006460 hydrolysis reaction Methods 0.000 description 2
230000001976 improved effect Effects 0.000 description 2
230000006872 improvement Effects 0.000 description 2
239000003112 inhibitor Substances 0.000 description 2
229960001614 levamisole Drugs 0.000 description 2
239000007788 liquid Substances 0.000 description 2
239000003550 marker Substances 0.000 description 2
VNWKTOKETHGBQD-UHFFFAOYSA-N methane Natural products C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
229930182817 methionine Natural products 0.000 description 2
238000000520 microinjection Methods 0.000 description 2
229940031815 mycocide Drugs 0.000 description 2
230000001069 nematicidal effect Effects 0.000 description 2
239000005645 nematicide Substances 0.000 description 2
229920001778 nylon Polymers 0.000 description 2
150000002894 organic compounds Chemical class 0.000 description 2
229920002866 paraformaldehyde Polymers 0.000 description 2
239000002245 particle Substances 0.000 description 2
239000012466 permeate Substances 0.000 description 2
239000000575 pesticide Substances 0.000 description 2
YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
230000026731 phosphorylation Effects 0.000 description 2
238000006366 phosphorylation reaction Methods 0.000 description 2
230000000704 physical effect Effects 0.000 description 2
230000002062 proliferating effect Effects 0.000 description 2
239000011347 resin Substances 0.000 description 2
229920005989 resin Polymers 0.000 description 2
230000008117 seed development Effects 0.000 description 2
230000011218 segmentation Effects 0.000 description 2
SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
238000010186 staining Methods 0.000 description 2
230000001954 sterilising effect Effects 0.000 description 2
239000005720 sucrose Substances 0.000 description 2
125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 2
230000007704 transition Effects 0.000 description 2
MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 2
229960003165 vancomycin Drugs 0.000 description 2
MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 2
230000003612 virological effect Effects 0.000 description 2
239000005723 virus inoculator Substances 0.000 description 2
LDVVMCZRFWMZSG-OLQVQODUSA-N (3ar,7as)-2-(trichloromethylsulfanyl)-3a,4,7,7a-tetrahydroisoindole-1,3-dione Chemical compound C1C=CC[C@H]2C(=O)N(SC(Cl)(Cl)Cl)C(=O)[C@H]21 LDVVMCZRFWMZSG-OLQVQODUSA-N 0.000 description 1
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
240000004510 Agastache rugosa Species 0.000 description 1
NXSFUECZFORGOG-CIUDSAMLSA-N Ala-Asn-Leu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(O)=O NXSFUECZFORGOG-CIUDSAMLSA-N 0.000 description 1
YHKANGMVQWRMAP-DCAQKATOSA-N Ala-Leu-Arg Chemical compound C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CCCN=C(N)N YHKANGMVQWRMAP-DCAQKATOSA-N 0.000 description 1
DPNZTBKGAUAZQU-DLOVCJGASA-N Ala-Leu-His Chemical compound C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N DPNZTBKGAUAZQU-DLOVCJGASA-N 0.000 description 1
CNQAFFMNJIQYGX-DRZSPHRISA-N Ala-Phe-Glu Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)C)CC1=CC=CC=C1 CNQAFFMNJIQYGX-DRZSPHRISA-N 0.000 description 1
DCVYRWFAMZFSDA-ZLUOBGJFSA-N Ala-Ser-Ala Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O DCVYRWFAMZFSDA-ZLUOBGJFSA-N 0.000 description 1
MSWSRLGNLKHDEI-ACZMJKKPSA-N Ala-Ser-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(O)=O MSWSRLGNLKHDEI-ACZMJKKPSA-N 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
235000005254 Allium ampeloprasum Nutrition 0.000 description 1
240000006108 Allium ampeloprasum Species 0.000 description 1
241000219318 Amaranthus Species 0.000 description 1
244000099147 Ananas comosus Species 0.000 description 1
235000007119 Ananas comosus Nutrition 0.000 description 1
240000007087 Apium graveolens Species 0.000 description 1
235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
235000010591 Appio Nutrition 0.000 description 1
108010039627 Aprotinin Proteins 0.000 description 1
241000218156 Aquilegia Species 0.000 description 1
108700005870 Arabidopsis RLK5 Proteins 0.000 description 1
108700005871 Arabidopsis RPS2 Proteins 0.000 description 1
101100277910 Arabidopsis thaliana DMC1 gene Proteins 0.000 description 1
101100460511 Arabidopsis thaliana LTP1 gene Proteins 0.000 description 1
101000650127 Arabidopsis thaliana Wax ester synthase/diacylglycerol acyltransferase 1 Proteins 0.000 description 1
235000017060 Arachis glabrata Nutrition 0.000 description 1
244000105624 Arachis hypogaea Species 0.000 description 1
235000010777 Arachis hypogaea Nutrition 0.000 description 1
235000018262 Arachis monticola Nutrition 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
HDHZCEDPLTVHFZ-GUBZILKMSA-N Asn-Leu-Glu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O HDHZCEDPLTVHFZ-GUBZILKMSA-N 0.000 description 1
OSZBYGVKAFZWKC-FXQIFTODSA-N Asn-Pro-Cys Chemical compound NC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CS)C(O)=O OSZBYGVKAFZWKC-FXQIFTODSA-N 0.000 description 1
241000972773 Aulopiformes Species 0.000 description 1
235000005781 Avena Nutrition 0.000 description 1
235000000832 Ayote Nutrition 0.000 description 1
241000218993 Begonia Species 0.000 description 1
235000016068 Berberis vulgaris Nutrition 0.000 description 1
241000335053 Beta vulgaris Species 0.000 description 1
240000007124 Brassica oleracea Species 0.000 description 1
235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
241000209200 Bromus Species 0.000 description 1
101000583086 Bunodosoma granuliferum Delta-actitoxin-Bgr2b Proteins 0.000 description 1
101150088200 CF-9 gene Proteins 0.000 description 1
208000003643 Callosities Diseases 0.000 description 1
235000002566 Capsicum Nutrition 0.000 description 1
239000005746 Carboxin Substances 0.000 description 1
235000009467 Carica papaya Nutrition 0.000 description 1
240000006432 Carica papaya Species 0.000 description 1
235000003255 Carthamus tinctorius Nutrition 0.000 description 1
244000020518 Carthamus tinctorius Species 0.000 description 1
241001674939 Caulanthus Species 0.000 description 1
235000007542 Cichorium intybus Nutrition 0.000 description 1
244000298479 Cichorium intybus Species 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
244000241235 Citrullus lanatus Species 0.000 description 1
235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
241001503991 Consolida Species 0.000 description 1
235000005956 Cosmos caudatus Nutrition 0.000 description 1
241000219112 Cucumis Species 0.000 description 1
235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
240000008067 Cucumis sativus Species 0.000 description 1
235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
240000004244 Cucurbita moschata Species 0.000 description 1
235000009854 Cucurbita moschata Nutrition 0.000 description 1
235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
241000612153 Cyclamen Species 0.000 description 1
235000012040 Dahlia pinnata Nutrition 0.000 description 1
244000033273 Dahlia variabilis Species 0.000 description 1
241000208296 Datura Species 0.000 description 1
108010082495 Dietary Plant Proteins Proteins 0.000 description 1
SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
108700022714 Drosophila pll Proteins 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
102000002322 Egg Proteins Human genes 0.000 description 1
108010000912 Egg Proteins Proteins 0.000 description 1
101710129170 Extensin Proteins 0.000 description 1
102000008175 FSH Receptors Human genes 0.000 description 1
108010060374 FSH Receptors Proteins 0.000 description 1
108090000331 Firefly luciferases Proteins 0.000 description 1
241000233866 Fungi Species 0.000 description 1
108010010803 Gelatin Proteins 0.000 description 1
241000245654 Gladiolus Species 0.000 description 1
SHERTACNJPYHAR-ACZMJKKPSA-N Gln-Ala-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCC(N)=O SHERTACNJPYHAR-ACZMJKKPSA-N 0.000 description 1
UKKNTTCNGZLJEX-WHFBIAKZSA-N Gln-Ser Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@@H](CO)C(O)=O UKKNTTCNGZLJEX-WHFBIAKZSA-N 0.000 description 1
240000005322 Gloxinia perennis Species 0.000 description 1
108010070675 Glutathione transferase Proteins 0.000 description 1
SSFWXSNOKDZNHY-QXEWZRGKSA-N Gly-Pro-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)CN SSFWXSNOKDZNHY-QXEWZRGKSA-N 0.000 description 1
ZZWUYQXMIFTIIY-WEDXCCLWSA-N Gly-Thr-Leu Chemical compound [H]NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(O)=O ZZWUYQXMIFTIIY-WEDXCCLWSA-N 0.000 description 1
KOYUSMBPJOVSOO-XEGUGMAKSA-N Gly-Tyr-Ile Chemical compound [H]NCC(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O KOYUSMBPJOVSOO-XEGUGMAKSA-N 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
241000234473 Hippeastrum Species 0.000 description 1
SVHKVHBPTOMLTO-DCAQKATOSA-N His-Arg-Asp Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(O)=O SVHKVHBPTOMLTO-DCAQKATOSA-N 0.000 description 1
MMFKFJORZBJVNF-UWVGGRQHSA-N His-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CN=CN1 MMFKFJORZBJVNF-UWVGGRQHSA-N 0.000 description 1
GBMSSORHVHAYLU-QTKMDUPCSA-N His-Val-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC1=CN=CN1)N)O GBMSSORHVHAYLU-QTKMDUPCSA-N 0.000 description 1
101000957437 Homo sapiens Mitochondrial carnitine/acylcarnitine carrier protein Proteins 0.000 description 1
101000610537 Homo sapiens Prokineticin-1 Proteins 0.000 description 1
101001129076 Homo sapiens Serine/threonine-protein kinase N1 Proteins 0.000 description 1
241000209219 Hordeum Species 0.000 description 1
240000005979 Hordeum vulgare Species 0.000 description 1
235000007340 Hordeum vulgare Nutrition 0.000 description 1
TWYOYAKMLHWMOJ-ZPFDUUQYSA-N Ile-Leu-Asn Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O TWYOYAKMLHWMOJ-ZPFDUUQYSA-N 0.000 description 1
HPCFRQWLTRDGHT-AJNGGQMLSA-N Ile-Leu-Leu Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O HPCFRQWLTRDGHT-AJNGGQMLSA-N 0.000 description 1
PHRWFSFCNJPWRO-PPCPHDFISA-N Ile-Leu-Thr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)O)N PHRWFSFCNJPWRO-PPCPHDFISA-N 0.000 description 1
241001495448 Impatiens <genus> Species 0.000 description 1
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 1
108010025815 Kanamycin Kinase Proteins 0.000 description 1
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
101150054956 LTP gene Proteins 0.000 description 1
235000003228 Lactuca sativa Nutrition 0.000 description 1
240000008415 Lactuca sativa Species 0.000 description 1
241000283986 Lepus Species 0.000 description 1
NTRAGDHVSGKUSF-AVGNSLFASA-N Leu-Arg-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O NTRAGDHVSGKUSF-AVGNSLFASA-N 0.000 description 1
JYOAXOMPIXKMKK-YUMQZZPRSA-N Leu-Gln Chemical compound CC(C)C[C@H]([NH3+])C(=O)N[C@H](C([O-])=O)CCC(N)=O JYOAXOMPIXKMKK-YUMQZZPRSA-N 0.000 description 1
QVFGXCVIXXBFHO-AVGNSLFASA-N Leu-Glu-Leu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O QVFGXCVIXXBFHO-AVGNSLFASA-N 0.000 description 1
PBGDOSARRIJMEV-DLOVCJGASA-N Leu-His-Ala Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](C)C(O)=O PBGDOSARRIJMEV-DLOVCJGASA-N 0.000 description 1
JVTYXRRFZCEPPK-RHYQMDGZSA-N Leu-Met-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CC(C)C)N)O JVTYXRRFZCEPPK-RHYQMDGZSA-N 0.000 description 1
GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 1
241000209082 Lolium Species 0.000 description 1
RQILLQOQXLZTCK-KBPBESRZSA-N Lys-Tyr-Gly Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)NCC(O)=O RQILLQOQXLZTCK-KBPBESRZSA-N 0.000 description 1
241000219480 Mesembryanthemum Species 0.000 description 1
235000009072 Mesembryanthemum Nutrition 0.000 description 1
SBSIKVMCCJUCBZ-GUBZILKMSA-N Met-Asn-Arg Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(O)=O)CCCNC(N)=N SBSIKVMCCJUCBZ-GUBZILKMSA-N 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
102100038738 Mitochondrial carnitine/acylcarnitine carrier protein Human genes 0.000 description 1
101100084404 Mus musculus Prodh gene Proteins 0.000 description 1
XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
KZNQNBZMBZJQJO-UHFFFAOYSA-N N-glycyl-L-proline Natural products NCC(=O)N1CCCC1C(O)=O KZNQNBZMBZJQJO-UHFFFAOYSA-N 0.000 description 1
101150117329 NTRK3 gene Proteins 0.000 description 1
241000244206 Nematoda Species 0.000 description 1
101100342977 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) leu-1 gene Proteins 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
BZQFBWGGLXLEPQ-UHFFFAOYSA-N O-phosphoryl-L-serine Natural products OC(=O)C(N)COP(O)(O)=O BZQFBWGGLXLEPQ-UHFFFAOYSA-N 0.000 description 1
238000012408 PCR amplification Methods 0.000 description 1
241000208181 Pelargonium Species 0.000 description 1
239000006002 Pepper Substances 0.000 description 1
241000182534 Pericallis hybrida Species 0.000 description 1
235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
244000046052 Phaseolus vulgaris Species 0.000 description 1
RAGOJJCBGXARPO-XVSYOHENSA-N Phe-Thr-Asp Chemical compound OC(=O)C[C@@H](C(O)=O)NC(=O)[C@H]([C@H](O)C)NC(=O)[C@@H](N)CC1=CC=CC=C1 RAGOJJCBGXARPO-XVSYOHENSA-N 0.000 description 1
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
235000016761 Piper aduncum Nutrition 0.000 description 1
240000003889 Piper guineense Species 0.000 description 1
235000017804 Piper guineense Nutrition 0.000 description 1
235000008184 Piper nigrum Nutrition 0.000 description 1
239000005924 Pirimiphos-methyl Substances 0.000 description 1
108010064851 Plant Proteins Proteins 0.000 description 1
208000020584 Polyploidy Diseases 0.000 description 1
235000000497 Primula Nutrition 0.000 description 1
241000245063 Primula Species 0.000 description 1
BWCZJGJKOFUUCN-ZPFDUUQYSA-N Pro-Ile-Gln Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(O)=O BWCZJGJKOFUUCN-ZPFDUUQYSA-N 0.000 description 1
108010079005 RDV peptide Proteins 0.000 description 1
239000013614 RNA sample Substances 0.000 description 1
108700008625 Reporter Genes Proteins 0.000 description 1
241000209051 Saccharum Species 0.000 description 1
240000000111 Saccharum officinarum Species 0.000 description 1
235000007201 Saccharum officinarum Nutrition 0.000 description 1
241001106018 Salpiglossis Species 0.000 description 1
241000228160 Secale cereale x Triticum aestivum Species 0.000 description 1
229920002684 Sepharose Polymers 0.000 description 1
LQESNKGTTNHZPZ-GHCJXIJMSA-N Ser-Ile-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(N)=O)C(O)=O LQESNKGTTNHZPZ-GHCJXIJMSA-N 0.000 description 1
HEUVHBXOVZONPU-BJDJZHNGSA-N Ser-Leu-Ile Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O HEUVHBXOVZONPU-BJDJZHNGSA-N 0.000 description 1
DINQYZRMXGWWTG-GUBZILKMSA-N Ser-Pro-Pro Chemical compound OC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 DINQYZRMXGWWTG-GUBZILKMSA-N 0.000 description 1
HAYADTTXNZFUDM-IHRRRGAJSA-N Ser-Tyr-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](C(C)C)C(O)=O HAYADTTXNZFUDM-IHRRRGAJSA-N 0.000 description 1
102100031206 Serine/threonine-protein kinase N1 Human genes 0.000 description 1
235000005775 Setaria Nutrition 0.000 description 1
241000232088 Setaria <nematode> Species 0.000 description 1
235000002597 Solanum melongena Nutrition 0.000 description 1
244000061458 Solanum melongena Species 0.000 description 1
235000002595 Solanum tuberosum Nutrition 0.000 description 1
244000061456 Solanum tuberosum Species 0.000 description 1
244000062793 Sorghum vulgare Species 0.000 description 1
238000002105 Southern blotting Methods 0.000 description 1
235000009337 Spinacia oleracea Nutrition 0.000 description 1
244000300264 Spinacia oleracea Species 0.000 description 1
241001112810 Streptocarpus Species 0.000 description 1
101150019400 TMK1 gene Proteins 0.000 description 1
101150094094 TMKL1 gene Proteins 0.000 description 1
241000736851 Tagetes Species 0.000 description 1
235000012308 Tagetes Nutrition 0.000 description 1
KZSYAEWQMJEGRZ-RHYQMDGZSA-N Thr-Leu-Val Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(O)=O KZSYAEWQMJEGRZ-RHYQMDGZSA-N 0.000 description 1
UMFLBPIPAJMNIM-LYARXQMPSA-N Thr-Trp-Phe Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)N[C@@H](CC3=CC=CC=C3)C(=O)O)N)O UMFLBPIPAJMNIM-LYARXQMPSA-N 0.000 description 1
102000003911 Thyrotropin Receptors Human genes 0.000 description 1
108090000253 Thyrotropin Receptors Proteins 0.000 description 1
108091023040 Transcription factor Proteins 0.000 description 1
102000040945 Transcription factor Human genes 0.000 description 1
235000019714 Triticale Nutrition 0.000 description 1
WACMTVIJWRNVSO-CWRNSKLLSA-N Trp-Asp-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC2=CNC3=CC=CC=C32)N)C(=O)O WACMTVIJWRNVSO-CWRNSKLLSA-N 0.000 description 1
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
239000006035 Tryptophane Substances 0.000 description 1
BSCBBPKDVOZICB-KKUMJFAQSA-N Tyr-Leu-Asp Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O BSCBBPKDVOZICB-KKUMJFAQSA-N 0.000 description 1
QPBJXNYYQTUTDD-KKUMJFAQSA-N Tyr-Met-Gln Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)N QPBJXNYYQTUTDD-KKUMJFAQSA-N 0.000 description 1
RWOGENDAOGMHLX-DCAQKATOSA-N Val-Lys-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C(C)C)N RWOGENDAOGMHLX-DCAQKATOSA-N 0.000 description 1
235000007212 Verbena X moechina Moldenke Nutrition 0.000 description 1
240000001519 Verbena officinalis Species 0.000 description 1
235000001594 Verbena polystachya Kunth Nutrition 0.000 description 1
235000007200 Verbena x perriana Moldenke Nutrition 0.000 description 1
235000002270 Verbena x stuprosa Moldenke Nutrition 0.000 description 1
235000010749 Vicia faba Nutrition 0.000 description 1
240000006677 Vicia faba Species 0.000 description 1
235000002098 Vicia faba var. major Nutrition 0.000 description 1
241000863480 Vinca Species 0.000 description 1
241000405217 Viola <butterfly> Species 0.000 description 1
208000027418 Wounds and injury Diseases 0.000 description 1
241000607479 Yersinia pestis Species 0.000 description 1
240000003307 Zinnia violacea Species 0.000 description 1
DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
239000002253 acid Substances 0.000 description 1
238000003916 acid precipitation Methods 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
239000013543 active substance Substances 0.000 description 1
239000008272 agar Substances 0.000 description 1
239000003905 agrochemical Substances 0.000 description 1
108010087924 alanylproline Proteins 0.000 description 1
230000001476 alcoholic effect Effects 0.000 description 1
235000011114 ammonium hydroxide Nutrition 0.000 description 1
230000003322 aneuploid effect Effects 0.000 description 1
208000036878 aneuploidy Diseases 0.000 description 1
101150008083 ant gene Proteins 0.000 description 1
230000000844 anti-bacterial effect Effects 0.000 description 1
239000000427 antigen Substances 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
229960004405 aprotinin Drugs 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
108010077245 asparaginyl-proline Proteins 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
108010092854 aspartyllysine Proteins 0.000 description 1
239000003899 bactericide agent Substances 0.000 description 1
PXXJHWLDUBFPOL-UHFFFAOYSA-N benzamidine Chemical compound NC(=N)C1=CC=CC=C1 PXXJHWLDUBFPOL-UHFFFAOYSA-N 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
230000005540 biological transmission Effects 0.000 description 1
210000000081 body of the sternum Anatomy 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
244000309466 calf Species 0.000 description 1
GYSSRZJIHXQEHQ-UHFFFAOYSA-N carboxin Chemical compound S1CCOC(C)=C1C(=O)NC1=CC=CC=C1 GYSSRZJIHXQEHQ-UHFFFAOYSA-N 0.000 description 1
239000000969 carrier Substances 0.000 description 1
230000003197 catalytic effect Effects 0.000 description 1
230000011712 cell development Effects 0.000 description 1
230000010261 cell growth Effects 0.000 description 1
239000013553 cell monolayer Substances 0.000 description 1
230000004663 cell proliferation Effects 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
235000010675 chips/crisps Nutrition 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
238000004140 cleaning Methods 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
238000011109 contamination Methods 0.000 description 1
235000012343 cottonseed oil Nutrition 0.000 description 1
230000010154 cross-pollination Effects 0.000 description 1
210000004748 cultured cell Anatomy 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
229930186364 cyclamen Natural products 0.000 description 1
230000024835 cytogamy Effects 0.000 description 1
230000003412 degenerative effect Effects 0.000 description 1
230000003413 degradative effect Effects 0.000 description 1
230000018044 dehydration Effects 0.000 description 1
238000006297 dehydration reaction Methods 0.000 description 1
229950006137 dexfosfoserine Drugs 0.000 description 1
QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
238000007598 dipping method Methods 0.000 description 1
238000002224 dissection Methods 0.000 description 1
238000001035 drying Methods 0.000 description 1
230000009977 dual effect Effects 0.000 description 1
230000005014 ectopic expression Effects 0.000 description 1
230000005611 electricity Effects 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
230000021759 endosperm development Effects 0.000 description 1
206010014881 enterobiasis Diseases 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
230000002349 favourable effect Effects 0.000 description 1
230000008175 fetal development Effects 0.000 description 1
239000004459 forage Substances 0.000 description 1
235000019253 formic acid Nutrition 0.000 description 1
238000009472 formulation Methods 0.000 description 1
238000004108 freeze drying Methods 0.000 description 1
239000012737 fresh medium Substances 0.000 description 1
108020001507 fusion proteins Proteins 0.000 description 1
102000037865 fusion proteins Human genes 0.000 description 1
239000008273 gelatin Substances 0.000 description 1
229920000159 gelatin Polymers 0.000 description 1
235000019322 gelatine Nutrition 0.000 description 1
235000011852 gelatine desserts Nutrition 0.000 description 1
230000035784 germination Effects 0.000 description 1
229960002989 glutamic acid Drugs 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
108010077515 glycylproline Proteins 0.000 description 1
108010037850 glycylvaline Proteins 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
239000003630 growth substance Substances 0.000 description 1
230000002650 habitual effect Effects 0.000 description 1
239000012145 high-salt buffer Substances 0.000 description 1
108010040030 histidinoalanine Proteins 0.000 description 1
108010092114 histidylphenylalanine Proteins 0.000 description 1
150000002460 imidazoles Chemical class 0.000 description 1
238000007654 immersion Methods 0.000 description 1
229930190166 impatien Natural products 0.000 description 1
238000009399 inbreeding Methods 0.000 description 1
230000002458 infectious effect Effects 0.000 description 1
ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 description 1
239000007924 injection Substances 0.000 description 1
238000002347 injection Methods 0.000 description 1
208000014674 injury Diseases 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
230000000968 intestinal effect Effects 0.000 description 1
239000007928 intraperitoneal injection Substances 0.000 description 1
229960000310 isoleucine Drugs 0.000 description 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
230000009191 jumping Effects 0.000 description 1
GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 1
108010052968 leupeptin Proteins 0.000 description 1
239000003446 ligand Substances 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
239000012669 liquid formulation Substances 0.000 description 1
239000012160 loading buffer Substances 0.000 description 1
230000033001 locomotion Effects 0.000 description 1
239000006166 lysate Substances 0.000 description 1
108010025153 lysyl-alanyl-alanine Proteins 0.000 description 1
238000005259 measurement Methods 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
239000011859 microparticle Substances 0.000 description 1
235000019713 millet Nutrition 0.000 description 1
239000003750 molluscacide Substances 0.000 description 1
WYXYBEOWQBGFQB-UHFFFAOYSA-N n-benzyl-7h-purin-2-amine Chemical class N=1C=C2NC=NC2=NC=1NCC1=CC=CC=C1 WYXYBEOWQBGFQB-UHFFFAOYSA-N 0.000 description 1
FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
235000016709 nutrition Nutrition 0.000 description 1
230000035764 nutrition Effects 0.000 description 1
230000000050 nutritive effect Effects 0.000 description 1
230000008520 organization Effects 0.000 description 1
210000004681 ovum Anatomy 0.000 description 1
238000002161 passivation Methods 0.000 description 1
235000020232 peanut Nutrition 0.000 description 1
230000000149 penetrating effect Effects 0.000 description 1
108010091212 pepstatin Proteins 0.000 description 1
FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 1
239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
230000002093 peripheral effect Effects 0.000 description 1
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
DCWXELXMIBXGTH-QMMMGPOBSA-N phosphonotyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-QMMMGPOBSA-N 0.000 description 1
239000011574 phosphorus Substances 0.000 description 1
229910052698 phosphorus Inorganic materials 0.000 description 1
BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 description 1
USRGIUJOYOXOQJ-GBXIJSLDSA-N phosphothreonine Chemical compound OP(=O)(O)O[C@H](C)[C@H](N)C(O)=O USRGIUJOYOXOQJ-GBXIJSLDSA-N 0.000 description 1
QHOQHJPRIBSPCY-UHFFFAOYSA-N pirimiphos-methyl Chemical group CCN(CC)C1=NC(C)=CC(OP(=S)(OC)OC)=N1 QHOQHJPRIBSPCY-UHFFFAOYSA-N 0.000 description 1
210000002826 placenta Anatomy 0.000 description 1
238000003976 plant breeding Methods 0.000 description 1
238000004161 plant tissue culture Methods 0.000 description 1
235000021118 plant-derived protein Nutrition 0.000 description 1
210000002706 plastid Anatomy 0.000 description 1
229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
230000001376 precipitating effect Effects 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
238000012545 processing Methods 0.000 description 1
108010077112 prolyl-proline Proteins 0.000 description 1
235000019260 propionic acid Nutrition 0.000 description 1
230000020167 protein autophosphorylation Effects 0.000 description 1
238000001742 protein purification Methods 0.000 description 1
210000001938 protoplast Anatomy 0.000 description 1
235000015136 pumpkin Nutrition 0.000 description 1
238000010926 purge Methods 0.000 description 1
IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
230000007420 reactivation Effects 0.000 description 1
102000005962 receptors Human genes 0.000 description 1
108020003175 receptors Proteins 0.000 description 1
238000011084 recovery Methods 0.000 description 1
238000009418 renovation Methods 0.000 description 1
230000000452 restraining effect Effects 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
238000010839 reverse transcription Methods 0.000 description 1
238000012552 review Methods 0.000 description 1
230000005070 ripening Effects 0.000 description 1
235000019515 salmon Nutrition 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000004062 sedimentation Methods 0.000 description 1
210000000582 semen Anatomy 0.000 description 1
230000019491 signal transduction Effects 0.000 description 1
210000003491 skin Anatomy 0.000 description 1
238000002791 soaking Methods 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
WESJNFANGQJVKA-UHFFFAOYSA-M sodium;2,3-dichloro-2-methylpropanoate Chemical compound [Na+].ClCC(Cl)(C)C([O-])=O WESJNFANGQJVKA-UHFFFAOYSA-M 0.000 description 1
239000007787 solid Substances 0.000 description 1
230000028070 sporulation Effects 0.000 description 1
239000007921 spray Substances 0.000 description 1
238000005507 spraying Methods 0.000 description 1
230000003019 stabilising effect Effects 0.000 description 1
238000003756 stirring Methods 0.000 description 1
239000000126 substance Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
238000005496 tempering Methods 0.000 description 1
238000004809 thin layer chromatography Methods 0.000 description 1
KUAZQDVKQLNFPE-UHFFFAOYSA-N thiram Chemical compound CN(C)C(=S)SSC(=S)N(C)C KUAZQDVKQLNFPE-UHFFFAOYSA-N 0.000 description 1
238000004448 titration Methods 0.000 description 1
238000012549 training Methods 0.000 description 1
238000013518 transcription Methods 0.000 description 1
230000035897 transcription Effects 0.000 description 1
230000005030 transcription termination Effects 0.000 description 1
238000010361 transduction Methods 0.000 description 1
230000026683 transduction Effects 0.000 description 1
238000013519 translation Methods 0.000 description 1
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
229960004799 tryptophan Drugs 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
108010002164 tyrosine receptor Proteins 0.000 description 1
238000012795 verification Methods 0.000 description 1
230000009385 viral infection Effects 0.000 description 1
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1

Images

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Botany (AREA)
Gastroenterology & Hepatology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Cell Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Physiology (AREA)
Developmental Biology & Embryology (AREA)
Environmental Sciences (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)

CN97194634A 1996-05-14 1997-05-13 无融合生殖种子的产生方法 Pending CN1218510A (zh)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GB9610044.1		1996-05-14
GBGB9610044.1A GB9610044D0 (en)	1996-05-14	1996-05-14	Improvements in or relating to organic compounds

Publications (1)

Publication Number	Publication Date
CN1218510A true CN1218510A (zh)	1999-06-02

Family

ID=10793673

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CN97194634A Pending CN1218510A (zh)	1996-05-14	1997-05-13	无融合生殖种子的产生方法

Country Status (15)

Country	Link
EP (1)	EP0915984A1 (pt)
JP (1)	JP2000510342A (pt)
KR (1)	KR20000011136A (pt)
CN (1)	CN1218510A (pt)
AR (1)	AR007130A1 (pt)
AU (1)	AU713130B2 (pt)
BR (1)	BR9709098A (pt)
CA (1)	CA2254839A1 (pt)
GB (1)	GB9610044D0 (pt)
HU (1)	HUP9901477A3 (pt)
IL (1)	IL126490A0 (pt)
PL (1)	PL329872A1 (pt)
RU (1)	RU2223320C2 (pt)
TR (1)	TR199802322T2 (pt)
WO (1)	WO1997043427A1 (pt)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US8183242B2 (en)	2004-12-31	2012-05-22	Piaoyang Sun	Aminopyrimidine compounds and their salts, process for preparation and pharmaceutical use thereof
CN103430831A (zh) *	2013-08-15	2013-12-11	黑龙江省农业科学院经济作物研究所	获得无融合生殖亚麻种子的方法
WO2021185358A1 (zh) *	2020-03-19	2021-09-23	中国科学院遗传与发育生物学研究所	一种提高植物遗传转化和基因编辑效率的方法

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU3781897A (en) *	1996-08-30	1998-03-19	Danny N. P. Doan	Endosperm and nucellus specific genes, promoters and uses thereof
GB9626858D0 (en) *	1996-12-24	1997-02-12	Gatsby Plant Science Innovatio	Transcriptional regulation in plants
US6452068B1 (en)	1998-01-28	2002-09-17	The Rockefeller University	Chemical inducible promoters used to obtain transgenic plants with a silent marker
US6262228B1 (en) *	1998-08-17	2001-07-17	Tularik Inc.	IRAK3 polypeptides and methods
GB9823098D0 (en) *	1998-10-22	1998-12-16	Novartis Ag	Organic compounds
EP1057891A1 (en)	1999-06-02	2000-12-06	Centrum Voor Plantenveredelings- En Reproduktieonderzoek	Use of the BNM3 transcriptional activator to control plant embryogenesis and regeneration processes
EP1094113A1 (en) *	1999-10-22	2001-04-25	Genetwister Technologies B.V.	Regeneration
CA2401858C (en)	2000-03-02	2015-12-01	Sudwestdeutsche Saatzucht Dr. H.R. Spath	Embryo sac-specific genes
CN1501978A (zh) *	2001-04-10	2004-06-02	��ɷ�	可诱导的无融合生殖
CA2451654A1 (en)	2001-06-22	2003-01-03	Ceres, Inc.	Chimeric histone acetyltransferase polypeptides
EP1382682A3 (en)	2002-07-17	2004-06-30	Expressive Research B.V.	Modulating developmental pathways in plants
JP4062356B2 (ja)	2004-04-16	2008-03-19	治北島	フリンジ咲きの花を有するニチニチソウおよびその育種方法
EP1621629A1 (en) *	2004-07-28	2006-02-01	Expressive Research B.V.	A method to increase pathogen resistance in plants
KR101317730B1 (ko)	2005-03-03	2013-10-15	리즈크 즈완 자드틸트 엔 자드한델 비.브이.	근접 후향 육종 방법
JP5818398B2 (ja) *	2005-03-03	2015-11-18	ライク・ズワーン・ザードテールト・アン・ザードハンデル・ベスローテン・フェンノートシャップ	逆子孫マッピング方法
US7550579B2 (en)	2005-04-29	2009-06-23	Pioneer Hi-Bred International, Inc.	Pericarp-preferred regulatory element
EP2119786A1 (en)	2008-05-13	2009-11-18	Expressive Research B.V.	Increased production of health-promoting compounds in plants
NL2004624C2 (en)	2010-04-28	2011-11-01	Stichting Dienst Landbouwkundi	A new glycosyltransferase protein and its role in the metabolism of phenylpropanoid volatiles in tomato.
KR101971016B1 (ko) *	2010-11-24	2019-04-23	넌헴스 비.브이.	이중 목적 수분수 수박
BR112014016774A2 (pt) *	2012-01-06	2020-10-27	Pioneer Hi-Bred International, Inc	método para produzir grande população de sementes, planta autorreprodutora e semente
US20240140998A1 (en) *	2021-03-02	2024-05-02	National Institute Of Advanced Industrial Science And Technology	Method for inducing embryogenesis of seed plant without fertilization, protein used therein, nucleic acid, vector, and recombinant seed plant in which embryo can be generated without fertilization

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
BR8807155A (pt) *	1987-07-31	1989-10-17	Maxell Hybrids Inc	Inducao assexual de esterilidade masculina transmissivel e apomixia em plantas
US5811636A (en) *	1995-09-22	1998-09-22	The United States Of America As Represented By The Secretary Of Agriculture	Apomixis for producing true-breeding plant progenies
US5710367A (en) *	1995-09-22	1998-01-20	The United States Of America As Represented By The Secretary Of Agriculture	Apomictic maize

1996
- 1996-05-14 GB GBGB9610044.1A patent/GB9610044D0/en active Pending
1997
- 1997-05-13 IL IL12649097A patent/IL126490A0/xx unknown
- 1997-05-13 KR KR1019980709296A patent/KR20000011136A/ko not_active Application Discontinuation
- 1997-05-13 HU HU9901477A patent/HUP9901477A3/hu unknown
- 1997-05-13 RU RU98122315/13A patent/RU2223320C2/ru not_active IP Right Cessation
- 1997-05-13 JP JP09540288A patent/JP2000510342A/ja active Pending
- 1997-05-13 TR TR1998/02322T patent/TR199802322T2/xx unknown
- 1997-05-13 CA CA002254839A patent/CA2254839A1/en not_active Abandoned
- 1997-05-13 CN CN97194634A patent/CN1218510A/zh active Pending
- 1997-05-13 AU AU29539/97A patent/AU713130B2/en not_active Ceased
- 1997-05-13 EP EP97923882A patent/EP0915984A1/en not_active Withdrawn
- 1997-05-13 PL PL97329872A patent/PL329872A1/xx unknown
- 1997-05-13 WO PCT/EP1997/002443 patent/WO1997043427A1/en not_active Application Discontinuation
- 1997-05-13 BR BR9709098A patent/BR9709098A/pt not_active Application Discontinuation
- 1997-05-14 AR ARP970102014A patent/AR007130A1/es unknown

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US8183242B2 (en)	2004-12-31	2012-05-22	Piaoyang Sun	Aminopyrimidine compounds and their salts, process for preparation and pharmaceutical use thereof
CN103430831A (zh) *	2013-08-15	2013-12-11	黑龙江省农业科学院经济作物研究所	获得无融合生殖亚麻种子的方法
CN103430831B (zh) *	2013-08-15	2014-08-13	黑龙江省农业科学院经济作物研究所	获得无融合生殖亚麻种子的方法
WO2021185358A1 (zh) *	2020-03-19	2021-09-23	中国科学院遗传与发育生物学研究所	一种提高植物遗传转化和基因编辑效率的方法

Also Published As

Publication number	Publication date
AR007130A1 (es)	1999-10-13
TR199802322T2 (xx)	1999-02-22
AU713130B2 (en)	1999-11-25
BR9709098A (pt)	1999-08-03
KR20000011136A (ko)	2000-02-25
JP2000510342A (ja)	2000-08-15
PL329872A1 (en)	1999-04-12
GB9610044D0 (en)	1996-07-17
WO1997043427A1 (en)	1997-11-20
EP0915984A1 (en)	1999-05-19
AU2953997A (en)	1997-12-05
HUP9901477A3 (en)	2001-11-28
RU2223320C2 (ru)	2004-02-10
IL126490A0 (en)	1999-08-17
CA2254839A1 (en)	1997-11-20
HUP9901477A2 (hu)	1999-09-28

Publication	Publication Date	Title
CN1218510A (zh)	1999-06-02	无融合生殖种子的产生方法
CN1170940C (zh)	2004-10-13	生长得到改造的植物以及获得此种植物的方法
CN1040024C (zh)	1998-09-30	对除莠剂敏感的植物或植物细胞进行转化的方法
CN1191568A (zh)	1998-08-26	包含蜕皮激素受体的基因开关
CN1309714A (zh)	2001-08-22	调节真核细胞中程序性细胞死亡的方法和手段
CN101029308A (zh)	2007-09-05	植物来源分子、其编码遗传序列及其用途
CN1236394A (zh)	1999-11-24	除草剂抗性植物
CN1228784A (zh)	1999-09-15	编码拟南芥的gai基因的核酸
CN1446227A (zh)	2003-10-01	新型成纤维细胞生长因子(fgf23)及其使用方法
CN1073717A (zh)	1993-06-30	编码杀虫剂蛋白的基因，用该基因转化的禾本科植物及其制备方法
CN1193098C (zh)	2005-03-16	利用条件雌性不育性生产杂种种子的方法
CN1555414A (zh)	2004-12-15	来源于植物的抗性基因
CN1878463A (zh)	2006-12-13	不育性植物体的生产方法以及使用此方法得到的植物体及其利用
CN1219885C (zh)	2005-09-21	转基因植物及其生产方法
CN1420932A (zh)	2003-05-28	由表达serk相互作用蛋白质实现无融合生殖
CN1751065A (zh)	2006-03-22	棉蚜(Aphis gossypii)抗性基因
CN1318974A (zh)	2001-10-24	受体样蛋白激酶rkn以及用其增加植物生长和产量的方法
CN1646005A (zh)	2005-07-27	具有改进形态发生的植物及其制备方法
CN1842592A (zh)	2006-10-04	赋予植物再分化能力的基因及其应用
CN1246464C (zh)	2006-03-22	可增强植物对渗透压的抵抗力的新转录因子
CN1535315A (zh)	2004-10-06	增强植物中胁迫耐受的组合物和方法
CN1860230A (zh)	2006-11-08	赋予对黄单胞菌引起的细菌性黑枯病的抗性的来自水稻的核酸
CN1430671A (zh)	2003-07-16	植物油菜素类固醇敏感性－相关基因及其用途
CN1289523C (zh)	2006-12-13	水稻钾、钠离子转运基因及其应用
CN1379783A (zh)	2002-11-13	通过功能性地抑制植物细胞周期蛋白抑制剂基因增加植物细胞增殖的方法

Legal Events

Date	Code	Title	Description
1999-06-02	C06	Publication
1999-06-02	PB01	Publication
1999-08-18	C10	Entry into substantive examination
1999-08-18	SE01	Entry into force of request for substantive examination
2002-12-11	ASS	Succession or assignment of patent right	Owner name: SINGENTA PARTICIPATION AG Free format text: FORMER OWNER: NOVANNIS COMPANY Effective date: 20021017
2002-12-11	C41	Transfer of patent application or patent right or utility model
2002-12-11	TA01	Transfer of patent application right	Effective date of registration: 20021017 Address after: Basel Applicant after: Xingenta Shara Co., Ltd. Address before: Basel Applicant before: Novartis AG
2005-01-26	C02	Deemed withdrawal of patent application after publication (patent law 2001)
2005-01-26	WD01	Invention patent application deemed withdrawn after publication