CN1209456C - Hair growth promoter and its preparing process and application - Google Patents
Hair growth promoter and its preparing process and application Download PDFInfo
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- CN1209456C CN1209456C CN 01114351 CN01114351A CN1209456C CN 1209456 C CN1209456 C CN 1209456C CN 01114351 CN01114351 CN 01114351 CN 01114351 A CN01114351 A CN 01114351A CN 1209456 C CN1209456 C CN 1209456C
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- GMMAPXRGRVJYJY-UHFFFAOYSA-J tetrasodium 4-acetamido-5-hydroxy-6-[[7-sulfonato-4-[(4-sulfonatophenyl)diazenyl]naphthalen-1-yl]diazenyl]naphthalene-1,7-disulfonate Chemical compound [Na+].[Na+].[Na+].[Na+].OC1=C2C(NC(=O)C)=CC=C(S([O-])(=O)=O)C2=CC(S([O-])(=O)=O)=C1N=NC(C1=CC(=CC=C11)S([O-])(=O)=O)=CC=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 GMMAPXRGRVJYJY-UHFFFAOYSA-J 0.000 description 1
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to a substance for promoting hair growth, a preparation method of the substance for promoting hair growth, an agent for promoting hair growth by using the substance for promoting hair growth as an effective ingredient, and the application of the agent. The substance for promoting hair growth is product obtained by that saccharomyces cerevisiae Hansen No. 1 promotion fur CCTCC M201024 bacterial strains are cultured and fermented in a culture medium Gibco 1640 and then are sterilized and filtered. The preparation method comprises cultivation, fermentation, filtration and sterilization. The substance for promoting hair growth has the function of promoting hair growth, and can be used for treating alopecia and increasing the yield of animal hair, such as sheep hair, rabbit hair, etc. The substance for promoting hair growth is safe and is non-poisonous.
Description
Technical field
The invention relates to a kind of hair growth promoter and its preparation method, and be the short hair growth agent and the application thereof of effective ingredient with this hair growth promoter.
Background technology
At present, baldness patient accounts for adult's ratio and is about 10%, but does not still have the definite effective medicine and method is treated it, and method and the medicine of seeking definite effective treatment baldness are the secular hopes of people, and have paid unremitting effort in this field.In addition, the whole world constantly increases the demand of animal wools such as wool, the rabbit hair, and the major measure of tackling expanded demand in the past is to increase to raise the quantity of producing the hair animal, enlarges raising scale.Yet, because environmental disruption is serious day by day, the restriction of factors such as deterioration of grasslands aggravation, it is no longer feasible that the animal rearing of employing expansion product hair increases the way of animal wool supply, must ward off his footpath in addition.
Summary of the invention
The object of the present invention is to provide a kind of hair growth promoter that utilizes microorganisms producing, make it have the effect of the hair growth of promotion, the output that can be used for treating baldness and increase animal wools such as wool, the rabbit hair, and safety non-toxic.
The microorganism of using:
Being used for producing hair growth promoter of the present invention is No. 1, the short hair of cereuisiae fermentum (Saccharomyces cerevisiaeHansen) (hereinafter to be referred as No. 1 bacterial strain of short hair), get through 118 mutagenesis, it has the ability that produces hair growth promoter of the present invention, this bacterial classification in July 8 calendar year 2001 in China's typical culture collection center preservation, deposit number is CCTCC M201204.
No. 1 bacterial strain of short hair has following character
1. morphological specificity
This bacterial strain is 28 ℃ of cultivations on the sabouraud's agar base, form soft and wet bacterium colony, and oyster white, cellular form are garden shape, ovum garden shape, and ellipse garden shape, its size is 3～6 * 5～10 microns, and precipitation is arranged in malt juice liquid medium, son bundle spore garden shape, level and smooth.
2. biochemical characteristic:
To glucose, fructose, N.F,USP MANNITOL, semi-lactosi, sucrose, maltose fermentation.
To close disaccharides nonfermented
Do not utilize nitrate
Cultivate and process for purification:
With above-mentioned production bacterium, use Gibco 1640 culture medium culturing and can make hair growth promoter of the present invention.Concrete operations are: with the above-mentioned bacterial classification unpacking of freeze-drying preservation, recovery, carry out strain identification in 28 ℃ of cultivations after 48 hours with sabouraud culture medium, the preferred high bacterial strain of promotes growth vigor carries out seed culture with Gibco 1640 substratum, 220 rev/mins of shaking tables, 28 ℃ 48～72 hours, be loaded on fermentor tank, at the Gibco substratum, cultivate 24h under 28 ℃, 300 rev/mins, stir culture condition, remove by filter thalline with the sheet frame filter again and promptly make hair growth promoter of the present invention, the short hair biochemical substances that makes is placed under the cold condition preserve.
Short hair growth agent
With hair growth promoter of the present invention is effective ingredient, can be mixed with the preparation of the function with short hair growth, is 0.1～1% as the proportioning of growth-promoting substance of the present invention in preparation of effective ingredient, generally with water as thinner.
Growth-promoting substance of the present invention has the effect that promotes cell, animal wool and human hair growth.
Test 1:
Material: NIH3T3
24 hole porous culture plates
Method: 1. well-grown NIH3T3 cell dilution is become 1 * 10
5/ ml cell, every hole adds 100ul;
2. every hole adds 0.5ml 1640 nutritive mediums (containing 10% deactivation bovine serum);
3. wherein three holes add 0.5% growth promoter 100ul of the present invention as test group;
4. in addition each Ensure Liquid liquid 100ul of three holes is in contrast.
Test-results: test group: cell boundary is clear, and the cell attachment growth is intensive, and quantity increases, and the cell transparency increases, and the cell endoparticle is few, and can indistinctly see nucleus; Control group: cell quantity increases not obvious, and is adherent relatively poor, and the cell endoparticle is more, and transparency is relatively poor.
Test 2
Material: mouse, Kunming kind, heavy 25g
Method: get 6 of mouse, 3 is test group, and 3 is control group;
1. earlier the mouse back hair is cut only with scissors;
2. test group is coated with the short hair growth preparation with the present invention of 1: 1000, and evenly coating makes the full back of the body moistening, once a day, is coated with altogether 3 times, and control group is coated with salt solution;
Test-results: test group back part ramp in 1～2 day, 3 days is the topped full back of the body.Control group: treat just to become mildewed after 7 days.
Test 3
The happy hair decorations factory that improves looks tests 100 routine seborrheic alopecia patients through the sky, Shanghai, 100% hair tonic as a result, and chief's hair luster brilliant black, hardness is good.
Test 4
(1) acute toxicity does not appear in acute toxicity test abdominal injection 2.5ml/kg maximal dose, and animal all survives, hair color, diet, activity, the no abnormal variation of body weight, LD50＞25ml/kg.
(2) the continuous gastric infusion of long term toxicity test is 3 months, and body weight, routine blood test, routine urinalysis, liver function, kidney merit and important organ pathologic finding there is no unusually.
(3) Salmonella reversion test Salmonella reversion test feminine gender, no mutagenicity.
(4) each test group rate of teratosperm of sperm malformation test is close with negative control group, no significant difference, and confirming does not have the sexual cell genetic damage effect that causes.
(5) micronucleus test micronucleus test feminine gender is not had a chromosome aberration effect.
(6) local application's toxicity research
(a) vaginal mucomembranous irritant test: the rabbit local mucous membrane is not seen obvious stimulation effects such as hyperemia, redness, oedema.
(b) skin anaphylactic test result: the said preparation sensitization is extremely low.
Can prove according to above test, with hair growth promoter of the present invention is that the short hair growth agent of effective ingredient is the short hair growth agent of a kind of definite effective, and the people has the growth that promotes animal wool, the effect of treatment human body seborrheic alopecia, and safety non-toxic is to skin zero damage.
Embodiment
Embodiment 1:
With No. 1 CCTCCM201024 bacterial classification unpacking of the short hair of the cereuisiae fermentum (Saccharomyces cerevisiae Hansen) of freeze-drying preservation, recovery, carry out strain identification in 28 ℃ of cultivations after 48 hours with sabouraud culture medium, the preferred high bacterial strain of promotes growth vigor carries out seed culture with Gibco 1640 substratum, 220 rev/mins of shaking tables, 28 ℃ 48～72 hours, be loaded on fermentor tank, at the Gibco1640 substratum, 28 ℃, 300 rev/mins, cultivate 24h under the stir culture condition, remove by filter thalline with the sheet frame filter again and promptly make hair growth promoter of the present invention, with the short hair biochemical substances that makes place under the cold condition preserve standby.
Embodiment 2:
In 1 milliliter of hair growth promoter of the present invention, add water to 1000 milliliters, what promptly make 1:1000 is the short hair growth agent of effective ingredient with hair growth promoter of the present invention, has the effect that promotes cell, animal wool and human hair growth, the hair growth that can be used for treating the human body alopecia and promote animals such as rabbit, sheep.The using method of treatment alopecia is: cleans the part of alopecia, and fully massages or steam the part, wipe the grease at alopecia position then with paper, embrocate short hair growth agent of the present invention with steam Dong with hand, and massage, a Wednesday is inferior, and be a course of treatment January.Be used to promote the using method of animal wool growth to be: the rabbit or the sheep of cutting hair with short hair growth agent drip washing of the present invention
Claims (6)
1. No. 1, the short hair of the mutagenic strain cereuisiae fermentum of a saccharomyces cerevisiae (Saccharomyces cerevisiaeHansen), and its preserving number is CCTCC M201024.
2. hair growth promoter, it is characterized in that it is with No. 1 CCTCC M201024 bacterial classification unpacking of the short hair of the cereuisiae fermentum (Saccharomyces cerevisiae Hansen) of freeze-drying preservation, recovery, carry out strain identification in 28 ℃ of cultivations after 48 hours with sabouraud culture medium, the preferred high bacterial strain of promotes growth vigor carries out seed culture with Gibco 1640 substratum, 220 rev/mins of shaking tables, 28 ℃ 48-72 hour, be loaded on fermentor tank, at the Gibco substratum, 28 ℃, 300 rev/mins, cultivate 24h under the stir culture condition, remove by filter resulting product behind the thalline with the sheet frame filter again.
3. the preparation method of a hair growth promoter may further comprise the steps:
(1) with No. 1 CCTCC M201024 bacterial classification unpacking of the short hair of the cereuisiae fermentum (Saccharomyces cerevisiae Hansen) of freeze-drying preservation, recovery, carry out strain identification in 28 ℃ of cultivations after 48 hours with sabouraud culture medium, the preferred high bacterial strain of promotes growth vigor carries out seed culture with the Gibco1640 substratum, 220 rev/mins of shaking tables, 28 ℃ 48-72 hour, be loaded on fermentor tank, at the Gibco substratum, cultivate 24h under 28 ℃, 300 rev/mins, stir culture condition;
(2) remove by filter thalline with the sheet frame filter again and promptly get hair growth promoter.
4. short hair growth agent is characterized in that it is is effective constituent with the described hair growth promoter of claim 2.
5. a short hair growth agent is characterized in that it is that the hair growth promoter of producing with the described method of claim 3 is an effective constituent.
6. the described growth-promoting substance of claim 2 is used for the treatment of depilation alopecia and the application that promotes in the animal wool growth preparation in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01114351 CN1209456C (en) | 2001-07-13 | 2001-07-13 | Hair growth promoter and its preparing process and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01114351 CN1209456C (en) | 2001-07-13 | 2001-07-13 | Hair growth promoter and its preparing process and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1396256A CN1396256A (en) | 2003-02-12 |
| CN1209456C true CN1209456C (en) | 2005-07-06 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01114351 Expired - Fee Related CN1209456C (en) | 2001-07-13 | 2001-07-13 | Hair growth promoter and its preparing process and application |
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| Country | Link |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102318061B1 (en) * | 2018-05-31 | 2021-10-28 | (주)진바이오텍 | Oat fermentation for companion animal |
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2001
- 2001-07-13 CN CN 01114351 patent/CN1209456C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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