CN1206833A - Rapidly measure components of cell layers - Google Patents

Abstract

The anticoagulated whole blood sample contained in the transparent tube is centrifuged to separate various blood cells from other components by gravity separation due to a difference in specific gravity. An insert or float is provided in the tube for elongating some of the separated component layers. The various component layers can be measured in the separation step using a linear image dissector cycle that measures the different fluorescence in the several component layers. Illumination of the blood sample tube as it is centrifuged causes the blood component layer to fluoresce. Gravity separation and quantification of the blood sample component layers is performed during centrifugation of the sample tubes. The final concentration of the component layers can be determined directly during centrifugation or can be extrapolated by performing a number of sequential measurements before the final concentration is reached.

Description

Rapidly measure components of cell layers

The present invention relates to an assembly for rapidly determining the volume of a centrifuged layer of material. The assembly of the present invention is particularly suitable for counting and measuring blood components of centrifuged blood samples of anticoagulated whole blood. Components of the present invention are used to implement the concurrently submitted "Method for Rapid Measurement of Cell Layers".

A method for blood count measurement of centrifuged blood samples from anticoagulated whole blood has been reported in the scientific literature, and also disclosed in Stephen C. Wardlaw et al. A method for conveniently measuring layers of certain blood cells and other components. In the method proposed in the patent, a sample of anticoagulated whole blood is centrifuged in a precision capillary tube containing a plastic float. The float causes linear expansion of certain cell layers and platelet layers.

When implementing the method of the above-mentioned patent, the sample is centrifuged at 12,000 rpm for about 5 minutes, and then the swelling length of the blood cell layer and platelet layer is measured. One of the problems with this method is that relatively high centrifugation speeds must be used to obtain reliable concentrated layers, especially platelet layers. If the concentration of the component layer is not complete or the concentration layer is not uniform, the results obtained by this method will not be accurate. Achieving these high centrifugation speeds requires expensive centrifugation equipment and increases the risk of tube rupture. Another problem is that there must be a centrifugation time of at least five minutes, which is undesirable in many medical settings. Another problem with this method is that the operator must remove the tube from the centrifuge table and place it in the reader. Since this operation must be completed within a limited time after centrifugation, the operator must keep a close eye on the sample, which is not only inefficient, but may also require the operator to come into contact with potentially dangerous samples.

It is desirable to be able to measure blood group stratification in a short period of time, at low centrifugation speeds, and/or to reduce the number of sample tube transfers.

The present invention proposes a device capable of rapidly determining the volume of each material layer during centrifugation of a gravity-separatable mixture sample, such as an anticoagulated whole blood sample. Furthermore, the invention relates to an assembly capable of determining the volume of bleeding components and counting blood components during the centrifugation of an anticoagulated whole blood sample, before the centrifugation step is completed. The assembly of the present invention provides an integrated centrifuge and readout device capable of centrifugation and readout, thereby simplifying the operation of measuring layers of matter as disclosed in the aforementioned US patents. The assembly of the present invention provides a method for dynamic analysis of blood cell concentrations. According to the principle of the present invention, the white blood cell and platelet layer can be quantitatively analyzed at a lower centrifugation speed, such as about 8000-10000 rpm (of course, a higher centrifugation speed, such as 12000 rpm, can also be used).

During the centrifugation of anticoagulated whole blood samples, gravity forms a blood cell layer, and there are two reaction forces, namely, the outward concentration of blood cells and other components formed in the blood sample and the inward penetration of plasma in the blood sample. filter. Due to the sedimentation of blood cells and other component layers formed in the blood sample during centrifugation, the layers of each component are concentrated and the height of the layer is reduced. At the same time, the liquid component (plasma) of the blood sample percolates through the concentrated layer.

In order for the cells to concentrate, the liquid components must move, but due to the continuous concentration of the cell layer, the percolation channel of the liquid components becomes more and more tortuous. Concentration of the cell layer develops rapidly at first, but as concentration increases and percolation becomes more difficult, the concentration of cells becomes slower and slower. Therefore, the rate of concentration is non-linear. Concentration rates vary widely between blood samples due to differences in fluid viscosity and/or other factors, so a single reading of the cell layer taken before the cell layer is fully concentrated cannot be used to extrapolate the final cell layer concentration degree. Therefore, the thickness (height) of a fully concentrated cell layer cannot be determined from a single reading of the thickness (or height) of the cell layer taken during centrifugation. This is the basis for the traditional method to determine the optimal centrifugation speed and time, therefore, the thickness of the cell layer is only measured after confirming that the cell layer is not further concentrated. This "fully concentrated" centrifugation time was considered the minimum time required before any centrifuged anticoagulated whole blood layer was measured.

The inventors have found that the inherent unpredictability of the final concentration of the blood cell layer can be overcome by taking several independent initial measurements of the thickness of the cell layer during centrifugation and then combining the obtained The data were fitted to a non-linear mathematical rule that predicts the thickness of a fully concentrated cell layer. This method does not require a maximum concentration of the material layer, in fact, the thickness of the fully concentrated cell layer can be predicted mathematically after measuring only 4 or 5 initial cell layer thicknesses. In addition, the method of the present invention can accurately calculate the maximum concentration degree of the blood cell layer within a relatively large centrifugal speed range, thereby obtaining the thickness of the fully concentrated cell layer. Therefore, it can be accurately predicted from the multiple thickness data of the cell layer measured during low-speed centrifugation of anticoagulated whole blood samples that a longer centrifugation time at a very high centrifugation speed (such as the speed required by the prior art) can be accurately predicted. The maximum concentration of the cell layer resulting from the centrifugation step over time.

The apparatus of the present invention comprises: a centrifugal separation apparatus; a fluorescent dye excitation light source; a photodetector and a microprocessor controller for controlling the operation of the apparatus and collecting data from the readings obtained by the apparatus. The above-mentioned light source is preferably a pulsed light source which periodically illuminates the blood sample being centrifuged in the blood sample tube. When the blood sample in the blood sample tube is illuminated, it causes certain blood cell components to fluoresce and the red blood cell layer to glow. various cell layers. By setting appropriate filters for the light source and photodetector, the light and fluorescence reflected by the material layer can be measured. Moreover, if the light source is placed opposite to the photodetector, or a reflector is placed behind the capillary, the light passing through the capillary can also be measured. The pulse of the light source is synchronized with the position of the sample tube during centrifugation, so that the sample tube is illuminated as it passes the photodetector. The optical instruments and optical filters used to implement the method of the present invention are generally similar to those disclosed in S.C. Wardlaw's US Patent No. 4,558,947 (granted on December 17, 1985), so the content of this patent is incorporated as a reference of the present invention.

If the above-mentioned dynamic method is used to analyze the blood sample, the images of the concentration degree of several blood sample component layers can be periodically intercepted during the centrifugation process, and the continuous component layer images are stored in the microprocessor control of the device. device. After capturing and storing a sufficient number of images (about 4 or 5 images), the microprocessor controller can calculate the maximum concentration of one or more component layers to be measured, and display its calculated value. So far, the centrifugation of the sample has just come to an end. The microprocessor controller controls the work of the above-mentioned device according to the following steps: start centrifugation; monitor the centrifuge speed; synchronize the light pulse with the centrifuge speed in the process; control the work of the photodetector; receive and store the readings of the component layer ; calculate the maximum concentration of component layer concentration, and display the count value or analysis value of the component; and close the centrifuge device. Therefore, the operator only needs to put the blood sample tube on the centrifuge table and start operating the device. In order to facilitate the operation and ensure the safety of the operator, the blood sample tube can be put into a special box of the general type described in the co-pending US patent application USSN 08/755363 (filing date is 1996.11.25) of the present invention.

On the other hand, if it is desired to determine the final concentration of cells after a fixed centrifugation time, one or more images can be intercepted during the continuous centrifugation process after a fixed centrifugation time, and the group can be analyzed accordingly. layered thickness. Therefore, the advantage of measuring the concentration of the cell layer can be realized without transferring the blood sample tube from the centrifugal table to an independent reader.

It is therefore an object of the present invention to provide an assembly which makes it possible to measure the thickness of the final material layer in the centrifuged mixture before the final layer thickness is actually achieved.

Another object of the present invention is to provide an assembly capable of reading the thickness of a material layer after a fixed centrifugation time while the sample is still centrifuged.

Yet another object of the present invention is to provide an assembly characterized in that the mixture to be analyzed is an anticoagulated whole blood sample.

Another object of the present invention is to provide an assembly characterized in that, during the centrifugation of the mixture, the data of the interface position of a plurality of successive initial layers are detected and stored, and the final layer thickness is calculated from the obtained interface data .

Yet another object of the present invention is to provide an assembly characterized in that the final thickness of a plurality of material layers during centrifugation of a sample can be calculated from initial layer thickness measurements.

From the following detailed description of the present invention in conjunction with the accompanying drawings, the above-mentioned and other objects and advantages of the present invention will be more apparent, in the accompanying drawings:

Fig. 1 is a simple view of a tube containing a mixture sample during centrifugation;

Fig. 2 is the dynamic curve diagram of the layer concentration that the mixture is made to the centrifugation time with layer thickness in the centrifugation process;

Fig. 3 is a graph showing how to calculate the final height of the platelet layer in the special case where the blood sample is centrifuged at two different rotational speeds, and is made from the reciprocal of the platelet layer height to the centrifugation time;

Figure 4 is a simplified perspective view of a blood sample testing device for carrying out the method of the present invention;

Figure 5 is a perspective view of the preferred embodiment of the centrifugal platen and transmission mechanism designed for the present invention;

6 is a partial perspective view of a clamping mechanism and a rotating mechanism for a sample tube for implementing the method of the present invention;

Fig. 7 is a cross-sectional view of a sample tube rotation mechanism for carrying out the method of the present invention.

Specific examples for implementing the present invention will be described below.

Referring to Figure 1, there is shown a tube 2 having a transparent side wall 4 and a closed bottom. Tube 2 is filled with a mixture of particle components suspended in liquid component 6 . Arrows A and B in Fig. 1 represent the kinetics of particle concentration and liquid percolation when the mixture of particle components and liquid components is centrifuged. When centrifugal separation is performed, the particle component will be separated from the liquid component 6 according to the law of gravimetric analysis, and an interface 8 will be formed at a certain moment. As the centrifugation continues, the interface 8 continues to descend, moving away from the upper surface 7 of the liquid component 6 more and more. It should be understood that, depending on the type of sample, more than one interface 8 may be formed when the sample is centrifuged.

It has been found that when the particle component/liquid position (or interface between different components) 8 is to be monitored throughout the separation process, the sequential position of the interface can be represented by a typical functional curve 10 as shown in Figure 2, It can be seen from curve 10 that the initial change of the interface position is relatively fast, but with the gradual concentration of the particle components, the movement of the interface position slows down significantly. At a certain point, the concentration of the particle components stops, at which point the separation of the particle components from the liquid components is declared complete. In the context of the present invention, this phenomenon of cessation of concentration of particle components is referred to as "maximum" concentration, which is indicated by dashed line 11 in FIG. 2 .

It should be noted that the same phenomenon occurs when centrifuging a complex mixture of particle components suspended in a liquid, such as anticoagulated whole blood. In this case, layers of particle components are formed gravimetrically, while the liquid component 6 percolates into the upper part of the centrifuge tube 2 . Blood is an example of such a complex mixture of particle components, because red blood cells in blood are heavier than granulocytes, lymphocytes, monocytes, and platelets in order of decreasing density, and all cells in a blood sample are heavier than those in a blood sample. When a sample of anticoagulated whole blood is centrifuged, the various cell/cell and cell/plasma interfaces will descend through the blood sample in the same general manner as shown in Figure 2. It should be noted that in certain cases, in complex substance mixtures (eg whole blood), the thickness of the intermediate layer may actually be thicker than the concentrated layer. This occurs when the rate of separation of the target component from the mixture exceeds the rate of concentration of the layer. In all cases, however, the same mathematical analysis and extrapolation can be performed and the results are the same as those for layers of reduced thickness.

The curve derived from the rate of change of the location of any particle component interface (ie, the thickness of the component layer) during centrifugation of the sample is essentially a hyperbola. This can be used to mathematically predict the maximum concentration of the particle component layer in a centrifuged sample, thereby predicting the maximum thickness or maximum volume of the particle component layer or multiple particle layers.

Fig. 3 shows the specific example of the same anticoagulated whole blood sample centrifuged at two different speeds (i.e. 8,000 rpm and 12,000 rpm), wherein the hyperbolic slope shown in Fig. 2 is due to A linear relationship is shown by plotting the reciprocal of the continuous centrifugation time against the continuous thickness measurements of the platelet-concentrated layer interface 8 at two different centrifugation speeds. Line 14 generally represents the change rate of platelet layer thickness when the sample is centrifuged at a high centrifugation speed (12,000 r/min); The change rate of platelet layer thickness during centrifugation, the centrifugal force G of the latter is only 40% of the former.

To determine the rate of change of the slope, a series of initial platelet layer thicknesses 16 are successively measured and a least squares fit is calculated to obtain an optimal trajectory 18 among the initial layer thickness data points 16 . To calculate the maximum concentration layer thickness, the regression function was extrapolated to a longest centrifugation time (in this case, the reciprocal value is 0 for an infinite centrifugation time). At this point, the intercept of the layer thickness variation velocity curve on the y-axis represents the maximum concentrated layer thickness. It should be noted that although the centrifugation speeds vary. But the end result is the same, and it takes only a very short time, eg, 2-3 minutes (compared to 5-10 minutes in the prior art) to get the required reading. Initial measurements can be taken while centrifugation continues.

Referring now to Figure 4, there is shown a schematic diagram of the combined centrifuge section and reader assembly (generally indicated by reference numeral 1). Assembly 1 comprises a centrifugation platen 3 with recesses 5 for holding transparent capillaries 9 . The capillary 9 can be placed directly in the groove 5, or it can be clamped in a box (not shown) of the kind described in the pending patent application USSN 08/755363 (filing date: 1996.11.25). Regardless of which method is used, at least one surface of the capillary 9 must be transparent to collect the desired optical information about the contents of the tube. The centrifugal platen 3 is rotated by a motor 13 controlled by an output line 21 from a microprocessor controller assembly 17 . The rotational speed of the motor 13 is monitored by the controller 17 through the line 19 in order to adjust the rotational speed of the motor and thus the rotational speed of the centrifugal platen 3 . When the rotation speed of the centrifugal platen 3 reaches its predetermined working speed (which may be about 8,000-12,000 rpm), the controller 17 will act according to the type of analysis required. It is preferable to read the concentration data of the material layer according to a certain centrifugation time, and the controller 17 powers the motor 13 according to the required fixed time interval, and then, while continuously performing centrifugation, reads the concentration data of the layer .

On the other hand, if it is desired to dynamically determine the concentration of the material layer, multiple sequential readings of the height of the gravity-concentrated blood cell layer in the capillary 9 should be taken. When the platen 3 rotates, the indexer 15 on the side of the platen 3 passes by the sensor 23 and senses it, and the sensor 23 transmits a signal to the programmable delayer 22 through the line 20 . The indexer 15 can be a permanent magnet, and the sensor 23 can be a Hall effect sensor. In addition, the indexer 15 can also be a reflective member located on the edge of the platen 3, and the sensor 23 can be an infrared emitting-receiving pair. In addition, the sensing means may also comprise a sensor arranged in the drive motor 13 (provided that the platen 3 can be rigidly fixed to the shaft of the drive motor 13).

After the controller 17 receives the signal from the adjacent sensor 23 after a predetermined time, the flash driver 24 triggers the flash tube 26 to emit a very short light pulse (preferably less than about 50 microseconds in duration), and is filtered by the filter. Optic and lens assembly 28 focuses the desired wavelength of light from flash tube 26 onto capillary 9 . If the flash tube 26 is located below the stage 3, the stage 3 is provided with an opening 3' between the sample tube 9 and the flash tube 26 and filter lens assembly 28. Since the rotating speed of the console plate 3 is precisely controlled by the controller 17 through the circuit 19, and the circumferential distance between the indexer 15 and the position of the capillary 9 is fixed, the delay time required for timely excitation of the flash tube 26 can be determined by the controller 17 and can be determined by the data. Bus 30 is expressed to control the operation of flash drive tube 24 .

When the capillary 9 is illuminated by the flash tube 26, the light reflected by the cell layer or the fluorescence emitted by the cell layer is focused by the lens assembly 32 on the linear image resolver 36 through the filter group 34, and the image resolver 36 is preferably Is a charge-coupled device (CCD) with at least 256 elements (preferably 5000 elements) for optimum optical resolution. By means of an actuator 38 (such as a solenoid or a stepper motor) controlled by the controller 17 through a line 40, the light of a suitable wavelength can be selected, so the actuator 38 can be selected from the filter according to the wavelength of light selected by the controller 17. An appropriate filter is selected from the optical filter group 34. Alternatively, variable filters can be used to provide light of the appropriate wavelength, or multiple CCDs with multiple sensors (each with its own specific filter). Suitable variable filters are commercially available from Cambridge Research and Instruments (Cambridge, MA.). Suitable CCDs are commercially available from Sony, Hitachi, and others, and CCDs are a common instrument component.

The electronic shutter in the CCD 36 is opened by the controller 17 through the line 42 immediately before receiving the flash from the flash tube 26 . Immediately after the flash, data from the CCD 36 is read into a digitizer 44 which converts the analog signal from each CCD cell into a digital signal. Then, the digitized data are transmitted to the controller 17 via the data bus 46, so that they can be immediately analyzed or stored for further processing in the controller 17. Thus, the optical information from the sample tube 9 can be collected and analyzed at any desired point in the centrifugation process using the method disclosed in the co-filed patent application "Method for Rapid Measurement of Cell Layers", or for other Purpose. And the above-mentioned mathematical calculation for obtaining the "maximum concentration" is performed by the controller 17.

Referring now to FIG. 5, the preferred embodiment of the centrifugal platen 3 for placing the flashlight source 26 and counter 44 shown in FIG. 4 on or below the platen 3 is shown. The above-mentioned platen 3 is generally disc-shaped and has an outer flange 50 and a base plate 52 . Attached to the base plate 52 of the deck is a central hub 54 which is closed by a cover 56 . A pair of diametrically opposed apertures 58 are formed in hub ring 54 . The sample tube 9 is mounted on the deck 3 with one end inserted into an aperture in the hub ring 54 and the other end inserted down into a slot 60 formed in a block 62 mounted on the deck flange 50 . The base plate 52 of the deck has an opening (not shown) covered by a transparent plate 64 . Balance weights 66 are arranged on two radially opposite sides of the transparent plate 64 for dynamically balancing the platen 3 . Setting the transparent plate 64 can respectively install the light source and the sensor on the top or bottom of the platen substrate 52, and can also make the module 1 use reflected light, fluorescent light or transmitted light to test the sample to obtain the required results. Figures 5 and 6 show a specific embodiment using a single capillary, however, it is also possible to install a sample tube at each diametrically opposite position on the platen 3, use the assembly 1 and change the time from indexing to flashing The analysis is performed on multiple sample tubes, and separate readings are obtained for each sample in the multiple tubes. The transmission shaft of the above-mentioned centrifugal motor 13 is shown with reference numeral 13'.

6 and 7 show the specific connection method of the drive shaft 13' of the motor with the platen 3 and the sample tube 9 and the platen hub ring 54. The transmission shaft 13' of the motor is fixed on the transmission disc 68 inside the hub ring 54. A pair of drive pins 70 are secured to the drive disc 68 and protrude from the opening 58 of the hub ring 54 . They are the only driving contacts between the motor drive shaft 13' and the platen 3. When the motor transmission shaft 13 ' drives the transmission disc 68 to rotate, it impels the transmission pin 70 to engage with the side of the hub ring opening 58 so that the hub ring 54 and the platen 3 rotate together with the transmission disc 68. Rod 72 is rotatably mounted on hub ring 54 and has at one end a collar 74 which receives one end of sample tube 9 . An elastic sealing ring (not shown) that can clamp one end of the sample tube 9 is placed inside the collar 74 . A toothed ratchet 76 is also mounted on the rod 72, and the ratchet 76 is operable to selectively rotate the collar 74 and the sample tube 9 in steps as follows. Mounted on drive plate 68 is a pawl 78 that is spring biased to engage the ratchet, and on hub ring cover 56 is mounted a leaf spring 80 to engage the ratchet. When the motor 13 drives the centrifugal motor transmission shaft 13' to rotate, the rotation of the transmission disc 68 will promote the engagement of the ratchet 78 with a tooth of the ratchet 76, and the leaf spring 80 will move downward to meet the diameter of the tooth in the ratchet 76. Engaging with the opposite teeth, as shown in Figure 7, in order to make the ratchet 76 and the sample tube 9 carry out selective rotation, power is intermittently supplied to the centrifugal motor 13 according to a certain time interval, so that the speed of the drive shaft 13' is slowed down briefly. Rotating speed. The momentum of the table 3 causes itself and its hub 54 to briefly rotate at a faster rate than the drive disc 68 to disengage the pawl 78 from the ratchet 76 and the drive pin 70 from the table hub 54. During this brief disengagement, the pawl 78 disengages from the ratchet tooth and moves into engagement with the next adjacent ratchet tooth. Then, the motor 13 is re-energized to full speed, thereby re-engaging the driving pin 70 with the hub ring 54, and causing the pawl 78 to drive the ratchet wheel 76 and the sample tube 9 to rotate one step clockwise. Therefore, when the ratchet 78 drives the next adjacent ratchet tooth, the rotation of the ratchet 76 causes the leaf spring 80 to mesh with the radially opposite next adjacent tooth on the ratchet 76, thereby making the ratchet 76 and the sample tube 9 Stabilized on a new rotational position, so the stepwise rotation of the sample tube 9 can make the image analysis tube 36 "see" the entire circumferential surface of the sample in the tube 9 when the sample tube is centrifuged, and make the system Circumferential variations in the position of the interface 8 of the descending sample component are considered. The above-mentioned pawl and ratchet type tube rotation mechanism is an invention of Michael R. Walters of Becton Dickinson and Company, and it is described in this application in order to meet the requirements of "best mode" stipulated in the Patent Law.

The concentration of the substance layer in the sample tube can be calculated from the multiple concentration data read during the centrifugation and the rotation of the sample tube, or after a predetermined rotation time sufficient to completely concentrate the substance layer to be measured , is calculated from the sequential readings taken while the centrifugation is continued during the rotation of the sample tube. In either case, the sample does not have to be transferred from the centrifuge device to a separate readout instrument, thus saving time and limiting direct operator contact with the sample. Another embodiment of a means to obtain the same reading involves the use of an intense continuous light source, such as a halogen lamp, that is turned on when the reading is taken. In order to make the reading speed fast enough to stabilize the image in the high-speed rotating sample tube, when the sample tube is aligned with the optical part, the electronic shutter on the CCD is only opened for a very short moment, such as one thousandth, two seconds or so. The indexer mentioned above can be used to replace the flash light source to make the CCD electronic shutter open synchronously. An advantage of this arrangement is that it does not require a flash tube and its associated circuitry. However, in order for the second embodiment described above to work properly, the centrifuge speed must be slowed to about 1,000 rpm in order to obtain a clear image of the tubes.

Since the disclosed embodiments of the present invention can be modified and changed without departing from the concept of the present invention, the present invention should not be limited by the above-mentioned embodiments, and the present invention should be defined by the appended claims.

Claims (15)

1. the system of the dense poly-degree of a component layers of measuring the target component in centrifugal multicomponent flowable materials sample of splendid attire in transparent pipe, described system comprises:

(a) centrifugal device comprises a platen and rotates the motor of platen, described platen be included in the material sample separate in the device of supporting coupon;

When (b) coupon on described platen is centrifugal, illuminate the light source of coupon;

(c) image dissector of a linearity, it cooperates with described centrifugal platen so that the light beam of sample imaging obtains an analytic signal from make test tube, described analytic signal is that representative is from the signal value along a plurality of points of coupon, when digitizing, these signals allow the distance of the adjacent interface of microprocessor detection and measurement target component layers;

(d) digitizer is connected with described image dissector, is used for described analog signal conversion is become digital signal; With

(e) microprocessor, be connected with described digitizer, be used to receive signal from digitizer, but the described digital signal of described microprocessor operation handlebar converts the quantization means of the dense poly-degree of described target component component layers to, therefore converts the volume of described target component component layers to.

2. the system of the dense poly-degree of a component layers of measuring the target component in centrifugal multicomponent flowable materials sample of splendid attire in transparent pipe, described system comprises:

(a) centrifugal device comprises a platen and rotates the motor of platen, described platen be included in the material sample separate in the device of supporting coupon;

When (b) coupon on described platen was centrifugal, the cycle was illuminated the light source of coupon;

(c) image dissector of a linearity, it cooperates with described centrifugal platen so that the light beam of sample imaging obtains an analytic signal from make test tube, described analytic signal is that representative is from the signal value along a plurality of points of coupon, when digitizing, these signals allow the distance of the adjacent interface of microprocessor detection and measurement target component layers;

(d) digitizer is connected with described image dissector, is used for described analog signal conversion is become digital signal; With

(e) microprocessor, be connected with described digitizer, be used to receive signal from digitizer, but the described digital signal of described microprocessor operation handlebar converts the quantization means of the dense poly-degree of described target component component layers to, therefore converts the volume of described target component component layers to.

3. according to the system of claim 2, it is characterized in that described microprocessor is operably connected so that control the control operation of described centrifugal motor with described centrifugal motor.

4. according to the system of claim 2, it is characterized in that described centrifugal platen comprises a detectable dividing apparatus, also comprise being used to survey the approaching sensor of described dividing apparatus, the feasible turned position that can measure described platen.

5. according to the system of claim 4, it is characterized in that also comprising a time-delay mechanism, be operably connected, so that only when platen is in a predetermined turned position, trigger described light source with described sensor and described light source.

6. according to the system of claim 4, it is characterized in that also comprising a filter assembly, comprise the wave filter that a plurality of and described digitizer matches, be used for the light of suitable wavelengths is fed to described digitizer.

7. according to the system of claim 6, it is characterized in that also comprising a filter selector that matches with described filter assembly, described filter selector is operably connected with described microprocessor so that allow described microprocessor to select suitable filters to be used in the described system.

8. according to the system of claim 2, it is characterized in that described light source be positioned at described platen below, the device of described supporting coupon is positioned at the top surface of described platen, and described platen comprises and passes light spare, allows the light of described light source to pass platen and is used for illuminating the interior content of coupon to coupon.

9. the system of the final dense poly-degree of a component layers of measuring the target component in centrifugal multicomponent flowable materials sample of splendid attire in transparent pipe, described system comprises:

(a) centrifugal device comprises a platen and rotates the motor of platen, described platen be included in the material sample separate in the device of supporting coupon;

When (b) coupon on described platen is centrifugal, illuminate the light source of coupon;

(c) image dissector of a linearity, it cooperates with described centrifugal platen so that the light beam of sample imaging obtains an analytic signal from make test tube, described analytic signal is that representative is from the signal value along a plurality of points of coupon, when digitizing, these signals allow the distance of the adjacent interface of microprocessor detection and measurement target component layers;

(d) digitizer is connected with described image dissector, is used for described analog signal conversion is become digital signal; With

(e) microprocessor, be connected with described digitizer, be used to receive signal from digitizer, but the described digital signal of described microprocessor operation handlebar converts the quantization means of the dense poly-degree of described target component component layers to, and can operate, calculate the final dense poly-degree of component of target component from the dense poly-measured value of a plurality of target component components before reaching final dense gathering.

10. according to the system of claim 9, it is characterized in that described microprocessor is operably connected so that control the control operation of described centrifugal motor with described centrifugal motor.

11., it is characterized in that described centrifugal platen comprises a detectable dividing apparatus, also comprise being used to survey the approaching sensor of described dividing apparatus the feasible turned position that can measure described platen according to the system of claim 9.

12., it is characterized in that also comprising a time-delay mechanism, be operably connected with described sensor and described light source, so that only when platen is in a predetermined turned position, trigger described light source according to the system of claim 11.

13., it is characterized in that also comprising a filter assembly according to the system of claim 11, comprise the wave filter that a plurality of and described digitizer matches, be used for the light of suitable wavelengths is fed to described digitizer.

14. system according to claim 13, it is characterized in that also comprising a filter selector that matches with described filter assembly, described filter selector is operably connected with described microprocessor so that allow described microprocessor to select suitable filters to be used in the described system.

15. system according to claim 9, it is characterized in that described light source be positioned at described platen below, the device of described supporting coupon is positioned at the top surface of described platen, and described platen comprises and passes light spare, allows the light of described light source to pass platen and is used for illuminating the interior content of coupon to coupon.

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