CN1205241A - Separating appts. of vertical electrophoresis charge particles - Google Patents
Separating appts. of vertical electrophoresis charge particles Download PDFInfo
- Publication number
- CN1205241A CN1205241A CN 97105169 CN97105169A CN1205241A CN 1205241 A CN1205241 A CN 1205241A CN 97105169 CN97105169 CN 97105169 CN 97105169 A CN97105169 A CN 97105169A CN 1205241 A CN1205241 A CN 1205241A
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- electrophoresis
- electrode
- pair
- separation equipment
- flow direction
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 23
- 239000002245 particle Substances 0.000 title claims abstract description 19
- 239000000463 material Substances 0.000 claims abstract description 26
- 238000000926 separation method Methods 0.000 claims abstract description 22
- 239000012530 fluid Substances 0.000 claims abstract description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 16
- 239000000741 silica gel Substances 0.000 claims description 9
- 229910002027 silica gel Inorganic materials 0.000 claims description 9
- 230000005684 electric field Effects 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 5
- 238000009826 distribution Methods 0.000 claims description 4
- 238000004587 chromatography analysis Methods 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 abstract description 4
- 238000005868 electrolysis reaction Methods 0.000 abstract 1
- 239000002904 solvent Substances 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000005012 migration Effects 0.000 description 4
- 238000013508 migration Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229910002804 graphite Inorganic materials 0.000 description 3
- 239000010439 graphite Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 210000003746 feather Anatomy 0.000 description 2
- 239000011810 insulating material Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- -1 albumen Proteins 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000009713 electroplating Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 229910001410 inorganic ion Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000012766 organic filler Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Electrostatic Separation (AREA)
Abstract
A charged particles separator with vertical electrophoresis is composed of chromatographic bearing material, electrophoresis electrodes, material feeder and collector, and features that the electrophoresis field is perpendicular to flowing direction of fluid to turn the direction of charged particles, which are finally separated out by sample collector. Said separator can be used for continuous separation of charged particles from fluid, analysis, preparing purpose and fluid electrolysis.
Description
The invention belongs to the industrial separation and analysis separation equipment of utilizing principle of electrophoresis, specially refer to the separation equipment of the vertical electrophoresis charged particle that can be used for preparation continuously and sewage disposal.
Migration behavior-the electrophoresis of charged particle (organic filler and inorganic ions) under electric field action, but by people be used for separating, the many charged species undergoes of Analysis and Identification.Common as paper electrophoresis, flaggy electrophoresis and gel electrophoresis are used for many years by people, but all are to be purpose with the compartment analysis, can not realize preparing continuously purpose.
The present invention combines sorbing material and electrophoresis electric field, use vertical electric field, be that direction of an electric field is vertical mutually with fluid flow direction, when the fluid streamwise moves, wherein contained charged particle is by the migration perpendicular to fluid flow direction, because the carrying capacity and the particle itself of various particles vary in size, migration speed is different and separate.Owing to the wash-out effect of fluid, particle also longshore current body flow direction moves, and moves along curve track at last simultaneously.Fluid is collected by special collection method, the material after the separation is discharged from different collection mouths, thereby realize separating continuously and preparation, and can be used for large-scale industrial production process and water treatment procedure (as desalination, desalinization etc.).
The objective of the invention is to propose to be used for various objectives, the continuous vertical electrophoresis separation equipment of different occasions.Its apparatus-form comprises and is used for trace protein, biology enzyme, the mini-plant that bioactivator is purified and separated, also comprise and be used for the main equipment that extensive inorganic salts separate, comprise by chromatograph paper being the plane vertical electrophoresis equipment of backing material, also comprise with silica gel etc. being the three-dimensional vertical electrophoresis equipment of backing material.
Technical conceive of the present invention is: a kind of separation equipment of vertical electrophoresis charged particle is by equipment box (4), and the chromatography backing material is a pair of or some to iontophoretic electrode (5), feeder (1), and sample divider (6) is formed.The chromatography backing material can be used chromatograph paper (2) or chromatograph silica gel (8).The distribution of iontophoretic electrode (5) can be the pair of planar electrode, a pair of stereo electrod or two pairs of stereo electrods, its main feature is that formed direction of an electric field of electrode and fluid flow direction are perpendicular, make charged particle when longshore current body flow direction moves, be subjected to the electric field force effect to take place to realize separating perpendicular to the skew of fluid flow direction.
When using chromatograph paper (2) is backing material, when iontophoretic electrode (5) is taked the plane vertical distribution, the solvent that will be used for wash-out is put in solvent groove (7), chromatograph paper (2) upper end is immersed in the solvent groove (7), and sample to be separated puts on the chromatograph paper center line or suitable position by feeder (1).Iontophoretic electrode (5) is clamped chromatograph paper (2), and electrode is fixing by support (3).Chromatograph paper feather edge vandyked, each tip is provided with a gatherer (6), collects different components.Can be in feeder (1) application of sample and from gatherer (6), take the material of separation continuously away continuously, realize separating continuously.The volatilization degree of looking used solvent determines whether and whole device need be arranged in the airtight case, makes its balance of steam.When making solvent, adopt airtight casing (4) with organic solvent; When making solvent, adopt open system with the aqueous solution.Wherein sample introduction speed and material composition number, institute's making alive, chromatograph paper width all has relation.For width is 30 centimetres chromatograph paper, and institute's making alive is generally 1～10 volt/centimetre.When 5 of number of components are following, sample size 10～50 ml/min; When 5 of number of components are above, sample size 1～10 ml/min.Simultaneously, the sawtooth number of chromatograph paper feather edge what, also relevant with separative efficiency, general sawtooth number can be consistent with number of components or more than number of components.The width of chromatograph paper influences separative efficiency equally, and the width of chromatograph paper increases and widens along with number of components, and the width of every component must satisfy the requirement of voltage after the width widen between 1～3 centimetre.
When being backing material with chromatograph silica gel (8), when in the casing of making by insulating materials (4), being symmetrical arranged a pair of iontophoretic electrode (5), be full of chromatograph silica gel (8) in the casing (4), bottom half evenly is divided into some grids (9), every grid element center has an osculum (10), each osculum is divided into a gatherer (6), material to be separated is added in casing (4) upper end by feeder (1), wherein not charged particle will obtain in interceptor(-ter), different electrical charged particles obtain in the gatherer near the opposite electrode plate, and its controlled condition is identical during with the papery backing material.
When being backing material with chromatograph silica gel (8), when in the casing of making by insulating materials (4), being symmetrical arranged two pairs of iontophoretic electrodes (5), be full of chromatograph silica gel (8) in the casing (4), bottom half evenly is divided into some grids (9), every grid element center has an osculum (10), each osculum is divided into a gatherer (6), material to be separated is added in casing (4) upper end by feeder (1), wherein not charged particle will obtain in interceptor(-ter), different electrical charged particles obtain in the gatherer near the opposite electrode plate, its controlled condition is identical during with the papery backing material, but its separating effect is excellent during than pair of electrodes.
The effect that the present invention can reach is:
1. the continuous Separation ﹠ Purification of micro-bioactivator (enzyme, hormone, albumen, DNA, RNA, plasmid etc.), for difficult separate substance, the two poles of the earth or multipolely separate continuously of can connecting are up to reaching the expection separation of level.
2. the amino acid whose recovery of large-scale production or by-product can selectively separate 18 seed amino acids step by step.
3. the processing of inorganic salts waste water, with the yin, yang ion migration of inorganic salts to electrode surface, at electrode surface generating electrodes reaction or precipitating metal (negative electrode) or be oxidized to other material (anode), thereby they are removed from waste water, the method can be used electroplating industry, inorganic chemicals industry, organic synthesis industry, desalinization etc.
Above-mentioned three kinds of separation equipments can be composed in series multi-stage separation, to satisfy various separation requirement.
Figure one is for being the vertical electrophoresis separation equipment structure chart of backing material with chromatograph paper
Figure two is for being the three-dimensional vertical electrophoresis separation equipment structure chart of backing material with silica gel
Figure three is for being the two-way three-dimensional vertical electrophoresis separation equipment structure chart of backing material with silica gel
Embodiment 1:
In the casing that 30 cm x, 30 cm x made from pvc material are 40 centimetres, place a pair of graphite electrode, in fill silica G, divide into 4 * 4 totally 16 gatherers, on establish the friction feeding pump sample to be separated be provided, under the condition of 30 volts of voltages, amino acid in the waste liquor from gourmet powder production is separated, under 0.5 liter of/hour condition of feed flow, pH value keeps the PH=2.5 of original production waste liquid, amino acid can be concentrated in 4 gatherers through the one-level electrophoresis, and be distribution clocklike, can obtain different types of amino acid sample.
Embodiment 2:
In the casing that 30 cm x, 30 cm x made from pvc material are 40 centimetres, place a pair of graphite electrode, in fill silica G, divide into 4 * 4 totally 16 gatherers, on establish the friction feeding pump sample to be separated be provided, under the condition of 30 volts of voltages, to PH is that 7 sodium chloride waste liquid separates, and under 0.5 liter of/hour condition of feed flow, can collect 4 class samples through the one-level electrophoresis, PH is respectively 1,3,6,13, can obtain acid, alkali and neutral no salt solution.
Embodiment 3:
In the casing that 30 cm x, 30 cm x made from pvc material are 40 centimetres, place a pair of graphite electrode, in fill silica G, divide into 4 * 4 totally 16 gatherers, on establish the friction feeding pump sample to be separated be provided, under the condition of 30 volts of voltages, to PH is that 7 sodium sulphate waste liquid separates, under 0.5 liter of/hour condition of feed flow, can collect 3 class samples through the one-level electrophoresis, be respectively NaOH, sulfuric acid, reach neutral no salt solution.
Claims (4)
1. the separation equipment of a vertical electrophoresis charged particle is characterized in that by equipment box (4), chromatography backing material (2) or (8), and a pair of or two pairs of electrodes (5), continuous charging device (1) and continuous sample gatherer (6) are formed.
2. separation equipment according to claim 1 is characterized in that the electrophoresis backing material can use chromatograph paper (2) or chromatograph silica gel (8).
3. separation equipment according to claim 1, the distribution that it is characterized in that iontophoretic electrode (5) can be the pair of planar electrode, a pair of stereo electrod or two pairs of stereo electrods, make formed direction of an electric field of electrode and fluid flow direction perpendicular, charged particle is subjected to the electric field force effect to take place to realize separating perpendicular to the skew of fluid flow direction when longshore current body flow direction moves.
4. separation equipment according to claim 3 is characterized in that being composed in series secondary or multi-stage separation process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97105169 CN1205241A (en) | 1997-07-14 | 1997-07-14 | Separating appts. of vertical electrophoresis charge particles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97105169 CN1205241A (en) | 1997-07-14 | 1997-07-14 | Separating appts. of vertical electrophoresis charge particles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1205241A true CN1205241A (en) | 1999-01-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 97105169 Pending CN1205241A (en) | 1997-07-14 | 1997-07-14 | Separating appts. of vertical electrophoresis charge particles |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1205241A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104096479A (en) * | 2014-07-17 | 2014-10-15 | 同济大学 | High-throughput continuous graphene oxide separation method and device |
| CN104174496A (en) * | 2014-08-22 | 2014-12-03 | 成都代代吉前瞻科技股份有限公司 | Electricity and bag combined deduster |
| CN108795753A (en) * | 2017-04-26 | 2018-11-13 | 苏州壹达生物科技有限公司 | A kind of device and method of encapsulation plane electrode chip |
| CN110639365A (en) * | 2019-09-12 | 2020-01-03 | 大连理工大学 | Preparative vertical flow electrophoresis system with support medium for mixed protein separation |
-
1997
- 1997-07-14 CN CN 97105169 patent/CN1205241A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104096479A (en) * | 2014-07-17 | 2014-10-15 | 同济大学 | High-throughput continuous graphene oxide separation method and device |
| CN104174496A (en) * | 2014-08-22 | 2014-12-03 | 成都代代吉前瞻科技股份有限公司 | Electricity and bag combined deduster |
| CN108795753A (en) * | 2017-04-26 | 2018-11-13 | 苏州壹达生物科技有限公司 | A kind of device and method of encapsulation plane electrode chip |
| CN110639365A (en) * | 2019-09-12 | 2020-01-03 | 大连理工大学 | Preparative vertical flow electrophoresis system with support medium for mixed protein separation |
| CN110639365B (en) * | 2019-09-12 | 2021-10-15 | 大连理工大学 | A preparative vertical flow electrophoresis system with support medium for mixed protein separation |
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