CN120174668B - Edible and medicinal fungus fiber-based sensing chip embedded paper and its preparation method and application - Google Patents
Edible and medicinal fungus fiber-based sensing chip embedded paper and its preparation method and applicationInfo
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- CN120174668B CN120174668B CN202510608621.6A CN202510608621A CN120174668B CN 120174668 B CN120174668 B CN 120174668B CN 202510608621 A CN202510608621 A CN 202510608621A CN 120174668 B CN120174668 B CN 120174668B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H13/00—Pulp or paper, comprising synthetic cellulose or non-cellulose fibres or web-forming material
- D21H13/10—Organic non-cellulose fibres
- D21H13/28—Organic non-cellulose fibres from natural polymers
- D21H13/30—Non-cellulose polysaccharides
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/20—Macromolecular organic compounds
- D21H17/21—Macromolecular organic compounds of natural origin; Derivatives thereof
- D21H17/24—Polysaccharides
- D21H17/25—Cellulose
- D21H17/26—Ethers thereof
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/20—Macromolecular organic compounds
- D21H17/21—Macromolecular organic compounds of natural origin; Derivatives thereof
- D21H17/24—Polysaccharides
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- D21H17/29—Starch cationic
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- D21J—FIBREBOARD; MANUFACTURE OF ARTICLES FROM CELLULOSIC FIBROUS SUSPENSIONS OR FROM PAPIER-MACHE
- D21J3/00—Manufacture of articles by pressing wet fibre pulp, or papier-mâché, between moulds
- D21J3/12—Manufacture of articles by pressing wet fibre pulp, or papier-mâché, between moulds of sheets; of diaphragms
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
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- C12R2001/645—Fungi ; Processes using fungi
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Abstract
本发明的目的是提供一种食药用真菌菌物纤维基感应芯片嵌入式纸张及其制备方法和应用，涉及微生物发酵技术领域，该食药用真菌菌物纤维基感应芯片嵌入式纸张的制备方法包括以下步骤：食药用真菌气生菌丝的培养，带有感应芯片的食药用真菌气生菌丝的干燥，食药用真菌菌物纤维的制备，食药用真菌菌物纤维纸浆的制备，嵌入感应芯片的食药用真菌菌物纤维纸张的制备。制备得到的菌物纤维基感应芯片嵌入式纸张具有良好的强度，并具有一定的防水性能，可作为包装纸应用于智能包装、物流追踪、医疗监测等多个领域。
The present invention aims to provide a paper with an embedded sensor chip based on edible and medicinal fungi fibers, as well as a preparation method and application thereof, relating to the field of microbial fermentation technology. The preparation method comprises the following steps: cultivating edible and medicinal fungi aerial hyphae, drying the edible and medicinal fungi aerial hyphae with the sensor chip, preparing edible and medicinal fungi fibers, preparing edible and medicinal fungi fiber pulp, and preparing edible and medicinal fungi fiber paper with the sensor chip embedded. The prepared paper with an embedded sensor chip based on edible and medicinal fungi fibers has good strength and certain waterproof properties, and can be used as wrapping paper in various fields such as smart packaging, logistics tracking, and medical monitoring.
Description
Technical Field
The invention relates to the technical field of microbial fermentation, in particular to edible and medicinal fungus fiber-based sensing chip embedded paper, and a preparation method and application thereof.
Background
Paper plays an irreplaceable role in society as a widely used material. The traditional paper is made from wood fiber as main raw material through strong alkali and strong acid treatment, bleaching, papermaking, drying, post-treatment and other processes, and has the problems of high wood resource consumption, environmental pollution and the like in the production process. As a novel renewable resource, the fungus fiber has the advantages of large-scale cultivation, high growth speed, easy degradation after use, environmental friendliness and the like, and has been widely paid attention to in the global scope.
The fungus fiber is mainly derived from edible and medicinal fungi and is a renewable resource. Compared with wood, the cultivation period of the edible and medicinal fungi is short, and the cultivation period of the edible and medicinal fungi hypha is shorter. The paper is prepared by taking edible and medicinal fungus fiber as a main raw material, so that the dependence of the paper industry on forests is reduced, and alkali and acid treatment is not needed. The bacterial fiber paper has good physical properties such as flexibility, strength, stiffness and the like. Meanwhile, the bacterial fiber is treated, and the paper preparation process is optimized, so that the performances of surface smoothness, glossiness and the like of the paper can be regulated, and the application field of the paper is expanded.
At present, the anti-counterfeiting and tracing of regular products are hot spot problems of interest to manufacturers and consumers. In view of the wide application foundation of paper in the fields of packaging, printing, labels and the like, the invention combines the fungus fiber paper with the induction chip, and can provide a new solution for enterprise product management and anti-counterfeiting. At present, most of the existing commodities use industrial glue to bond the induction chip and paper, which not only affects the performance of the chip, but also causes pollution to the environment. The edible and medicinal fungus fiber-based sensing chip embedded paper is novel intelligent environment-friendly paper, has the advantages of reproducibility, degradability, no pollution and the like, can realize the functions of fake detection, tracing, counting and the like, is embedded into fungus fiber paper, increases the concealment of the chip, gives more functions and application value to the paper, and can not influence the sensing function of the embedded chip even after being folded and rubbed for many times based on the unique physical properties of the fungus fiber paper, so that the function stability of the chip is ensured. Therefore, the invention can provide an effective method for enterprises in aspects of commodity fake detection, tracing, checking and the like, reduces the technical, manpower and time costs of the enterprises, has wide market application prospect and has good ecological benefit.
Disclosure of Invention
The invention provides a fiber-based sensing chip embedded paper of edible and medicinal fungus substances, a preparation method and application thereof, and a green paper preparation process is initiated, strong alkali and strong acid are not used, and the paper is environment-friendly; the prepared edible and medicinal fungus fiber-based sensing chip embedded paper is novel environment-friendly paper, has the advantages of being renewable and degradable, realizes natural embedding of the sensing chip in the forming process of the edible and medicinal fungus fiber-based sensing chip embedded paper, does not influence chip functions, and meanwhile, the edible and medicinal fungus fiber-based sensing chip embedded paper has good flexibility and elasticity, can play a role in buffering and protecting the sensing chip under the action of external forces such as folding, kneading and the like, ensures the functional stability of the sensing chip, and can realize the functions of fake detection, tracing, counting and the like.
According to one aspect of the invention, a preparation method of an embedded paper of a fiber-based sensing chip of edible and medicinal fungus substances is provided, and the method comprises the following steps:
step 1, culturing aerial hyphae of edible and medicinal fungi:
Inoculating the strain of the edible and medicinal fungi into a solid culture medium, wherein the inoculum size is 5% -10%, and placing the solid culture medium in a culture room for culture to obtain aerial hyphae of the edible and medicinal fungi;
Step 2, drying aerial hyphae of edible and medicinal fungi with sensing chip:
Placing a sensing chip with a clean surface in the edible and medicinal fungus aerial hypha, culturing for 1-3 days to obtain the edible and medicinal fungus aerial hypha with the sensing chip, taking out the edible and medicinal fungus aerial hypha with the sensing chip, and drying to obtain the dried edible and medicinal fungus aerial hypha with the sensing chip;
step3, preparing edible and medicinal fungus fiber:
taking out the sensing chip with the edible and medicinal fungi aerial hypha on the surface from the dried edible and medicinal fungi aerial hypha with the sensing chip for standby, then adding water into the rest dried edible and medicinal fungi aerial hypha for grinding to prepare edible and medicinal fungi fungus fiber liquid, and obtaining the edible and medicinal fungi fungus fiber after multiple centrifugation;
step 4, preparing edible and medicinal fungus fiber pulp:
adding water into edible and medicinal fungus fiber to prepare edible and medicinal fungus fiber suspension, adding sodium carboxymethyl cellulose to obtain suspension 1, stirring with a magnetic stirrer, adding cationic starch to obtain suspension 2, and stirring with a magnetic stirrer to obtain edible and medicinal fungus fiber pulp;
step 5, preparing edible and medicinal fungus substance fiber paper embedded with sensing chip:
Adding water into edible and medicinal fungus fiber pulp to prepare edible and medicinal fungus pulp suspension, carrying out suction filtration by a vacuum suction filter to obtain two edible and medicinal fungus fiber filter cakes, spreading one of the edible and medicinal fungus fiber filter cakes in a die, putting the sensing chip with the edible and medicinal fungus aerial hypha on the surface of the sensing chip in the step 3, putting the other edible and medicinal fungus fiber filter cake into the sensing chip to form an edible and medicinal fungus fiber filter cake embedded into the sensing chip, and putting the edible and medicinal fungus fiber filter cake into a paper sheet former to form, dehydrate and dry the edible and medicinal fungus fiber filter cake to obtain the edible and medicinal fungus fiber-based sensing chip embedded paper.
Preferably, the edible and medicinal fungi in the step 1 are selected from any one of ganoderma lucidum, phellinus linteus, lentinus Edodes, auricularia, hericium erinaceus, maitake Mushroom, and Poria.
Preferably, the preparation method of the solid culture medium in the step 1 comprises the steps of filling a culture bag with a solid culture medium raw material, punching a small hole in the center of a bag body of the culture bag, sterilizing for 20-120 min at 115-121 ℃, and cooling the solid culture medium to 25-30 ℃ to obtain the solid culture medium.
Preferably, the solid culture medium in the step 1 comprises solid culture medium raw materials and water, wherein the solid culture medium raw materials comprise 70% -78% of wood chips, 20% -26% of bran, 1% -2% of gypsum and 1% -2% of sugar.
Preferably, after the step 1, the method further comprises screening the aerial hyphae of the edible and medicinal fungi with white color, no abnormal color change and uniform and dense growth by adopting a naked eye observation method, and screening the aerial hyphae of the edible and medicinal fungi with a shape of robustness and a hyphae diameter of not less than 1 mu m by adopting a scanning electron microscope observation method.
Preferably, the specific method of the drying treatment in the step2 is that the aerial hypha of the edible and medicinal fungi with the induction chip is placed into a constant-temperature blast drying oven, the temperature is 45-65 ℃, and the drying treatment is carried out for 5-10 hours.
Preferably, after the step 2, the method further comprises the step of screening the dried aerial hyphae of the edible and medicinal fungi with the induction chip by adopting a reduced pressure drying method, wherein the moisture content of the aerial hyphae is not more than 4%.
The preparation method of the edible and medicinal fungus fiber in the step 3 comprises the steps of taking out a sensing chip with the surface covered with the edible and medicinal fungus aerial hypha from dried edible and medicinal fungus aerial hypha with the sensing chip for standby, adding water into the residual dried edible and medicinal fungus aerial hypha for grinding to prepare edible and medicinal fungus fiber liquid, placing the edible and medicinal fungus fiber liquid into a water bath kettle with the temperature of 45-65 ℃ and the rotation speed of 200-300 r/min for 60-120 min, placing the edible and medicinal fungus fiber liquid into a centrifuge for centrifugation at 3500-4500 r/min for 10-15 min, washing the centrifuged sediment with purified water, and centrifuging at 3500-4500 r/min for 10-15 min to obtain the sediment which is the edible and medicinal fungus fiber.
Preferably, in the step 3, the method for grinding the residual dry aerial hyphae of the edible and medicinal fungi by adding water comprises the steps of putting the dry aerial hyphae of the edible and medicinal fungi into a pulping machine, adding purified water to obtain aerial hyphae suspension of the edible and medicinal fungi, enabling the concentration of the aerial hyphae of the edible and medicinal fungi in the aerial hyphae suspension of the edible and medicinal fungi to be 40-60 g/100mL, setting the clearance distance of a grinding disc to be 1-5 mm, enabling the rotor power of a fly cutter to be 1.1kW, enabling the rotating speed of the fly cutter to be 1430-1470 r/min, and grinding the aerial hyphae suspension of the edible and medicinal fungi for 5-15 min to obtain the edible and medicinal fungi fiber liquid.
Preferably, after the step 3, the method further comprises screening the edible and medicinal fungus substance fibers by adopting a laser confocal fluorescence microscope, wherein the length of the edible and medicinal fungus substance fibers is not less than 20 mu m, and the width of the edible and medicinal fungus substance fibers is not more than 1 mu m.
Preferably, the specific preparation method of the edible and medicinal fungus fiber pulp in the step 4 comprises the steps of adding water into edible and medicinal fungus fiber to prepare edible and medicinal fungus fiber suspension, adding sodium carboxymethylcellulose to obtain suspension 1, stirring by a magnetic stirrer, stirring the suspension 1 at 45-65 ℃ at a rotating speed of 150-200 r/min for 15-45 min, adding cationic starch to obtain suspension 2, stirring by the magnetic stirrer, and stirring the suspension 2 at 45-65 ℃ at a rotating speed of 150-200 r/min for 15-45 min to obtain the edible and medicinal fungus fiber pulp.
Preferably, the concentration of the edible and medicinal fungus fiber in the step 4 in the edible and medicinal fungus fiber suspension is 30-60 g/100mL, the concentration of sodium carboxymethyl cellulose in the suspension 1 is 0.2-1.2 g/100mL, and the concentration of cationic starch in the suspension 2 is 0.4-1.4 g/100mL.
Preferably, after the step 4, the method further comprises the steps of screening the surface charge of the edible and medicinal fungus fiber paper pulp by using a potentiometer to be not less than 8mV, and screening the average particle size of the edible and medicinal fungus fiber paper pulp by using a particle sizer to be not less than 1mm.
Preferably, the concentration of the edible and medicinal fungus fiber in the edible and medicinal fungus paper pulp suspension in the step 5 is 0.7-1.4 g/100mL, the gram weight of the edible and medicinal fungus fiber filter cake embedded with the sensing chip in the step 5 is 50-100 g/m2, and the specific conditions of dehydration and drying in the step 5 are that the heating power is 10-20 kw, the drying temperature is 40-60 ℃ and the drying time is 12-24 h.
Preferably, after the step 5, the method further comprises the steps of screening the edible and medicinal fungus fiber-based sensing chip embedded paper by adopting a contact angle measuring instrument, screening the edible and medicinal fungus fiber-based sensing chip embedded paper by adopting a folding endurance tester, wherein the folding endurance tester can not influence the sensing function of the sensing chip after 100 times of folding, screening the edible and medicinal fungus fiber-based sensing chip embedded paper by adopting an electronic universal tester, wherein the tensile strength of the edible and medicinal fungus fiber-based sensing chip embedded paper is not less than 180MPa, screening the surface smoothness of the edible and medicinal fungus fiber-based sensing chip embedded paper by adopting a smoothness tester, screening the stiffness of the edible and medicinal fungus fiber-based sensing chip embedded paper by adopting a stiffness tester is not less than 0.1mNm, and screening the glossiness of the edible and medicinal fungus fiber-based sensing chip embedded paper by adopting a glossiness tester is not less than 70%.
According to another aspect of the invention, a paper prepared by the method for preparing the edible and medicinal fungus fiber-based sensing chip embedded paper is also provided.
According to another aspect of the invention, the invention also provides application of the edible and medicinal fungus fiber-based sensing chip embedded paper as packaging paper.
The method for preparing the paper by using the edible and medicinal fungus fiber as the raw material has the advantages of being beneficial to reducing dependence on traditional wood fiber, meeting the requirements of environmental protection and sustainable development, initiating the paper with the embedded edible and medicinal fungus fiber-based sensing chip for the first time, having good strength and certain waterproof performance, and ensuring the stability and reliability of the paper with the embedded edible and medicinal fungus fiber-based sensing chip. The edible and medicinal fungus fiber-based sensing chip embedded paper prepared by the invention can be applied to a plurality of fields such as intelligent packaging, logistics tracking, medical monitoring and the like, and has wide market prospect.
Drawings
FIG. 1 is a flow chart of an embodiment of the present invention.
FIG. 2 is an apparent morphology of aerial hyphae of Ganoderma lucidum.
FIG. 3 is a morphology of a scanning electron microscope of aerial hyphae of Ganoderma lucidum.
Fig. 4 is a schematic diagram of a sensing chip.
FIG. 5 is a schematic illustration of the surface of the sensor chip surrounded by and interwoven with aerial mycelia of Ganoderma lucidum.
FIG. 6 is a morphological diagram of a laser confocal fluorescence microscope of ganoderma lucidum fiber.
FIG. 7 is a diagram showing the morphology of a ganoderma lucidum fiber-based sensing chip embedded in a mold prior to molding.
FIG. 8 is a diagram showing the morphology of the ganoderma lucidum fiber-based sensor chip embedded paper before molding.
FIG. 9 is a diagram showing the morphology of the molded ganoderma lucidum fiber-based sensor chip embedded paper.
Detailed Description
Definition:
the term "edible and medicinal fungus fiber" as used herein refers to a type of dietary fiber existing in the cell wall of edible and medicinal fungus, and mainly consists of polysaccharide substances, including beta-glucan, chitin, cellulose and the like.
As used herein, the term "aerial mycelium" refers to a type of mycelium of a fungus that is edible and medicinal, and that extends into space from the mycelium after it grows in the medium. On a solid medium, aerial hyphae are visible to the naked eye and often cover the surface of the medium, giving a layer of fuzzy appearance. The aerial hypha has multiple important physiological functions, on one hand, the aerial hypha can be further extended and expanded to explore new nutrition sources and find suitable growth environments, and on the other hand, the aerial hypha can be differentiated to form propagation organs such as sporangium, conidiophore and the like when being developed to a certain stage, and the aerial hypha can be used for generating various asexual or sexual spores so as to realize propagation and propagation of edible and medicinal fungi. In the artificial culture process of edible and medicinal fungi, the growth condition of aerial hyphae is one of important indexes for judging whether culture conditions are proper. For example, if aerial hyphae grow too vigorously, the formation and development of fruiting bodies may be affected, which may be related to factors such as humidity, temperature, ventilation, etc. in the culture environment. Conversely, if the aerial hyphae grow poorly, this may mean that the nutrition is insufficient or the environmental conditions are unsuitable, and the culture conditions need to be adjusted.
The term "solid culture medium" as used herein refers to a solid substance which provides nutrition and habitat for the growth, development and propagation of edible and medicinal fungi, and which comprises the following components:
(1) The main nutrition source comprises a carbon source (such as glucose, sucrose, starch, wood dust, corncob and the like, and provides energy for the growth of edible and medicinal fungi and a carbon skeleton for synthesizing cell substances), and a nitrogen source (such as peptone, yeast powder, bean cake powder, bran, corn powder and the like), meets the requirement of fungi on nitrogen, and is used for synthesizing nitrogen-containing biomolecules such as protein, nucleic acid and the like.
(2) Inorganic salts, which are mineral elements necessary for providing growth of edible and medicinal fungi, such as phosphorus, potassium, magnesium, calcium and the like, are generally added with the inorganic salts such as monopotassium phosphate, magnesium sulfate, calcium sulfate and the like to meet the demands. These elements play an important role in cell metabolism, regulation of enzyme activity, etc.
(3) Growth factors, including vitamins, amino acids, nucleic acids, and the like, are organic substances that are essential for growth of the edible and medicinal fungi, but cannot be synthesized by themselves or are not synthesized in sufficient amounts. For example, yeast extract contains abundant growth factors such as B vitamins, and can promote growth and development of fungi.
(4) Coagulants, most commonly agar, which allows the medium to remain solid at normal temperature, provide a support structure for the growth of the hyphae of the fungus.
Example 1 of the present invention:
step 1, culturing ganoderma aerial hyphae:
The method comprises the steps of taking 936g of wood dust, 240g of bran, 12g of gypsum and 12g of sugar as solid culture matrix raw materials, uniformly stirring, adding 1500 g of purified water to obtain a solid culture matrix, enabling the water content in the solid culture matrix to be 55%, filling the solid culture matrix into a culture bag, enabling the culture bag to be a plastic bag for cultivation, punching a small hole in the center of a bag body of the culture bag, sterilizing the culture bag at 121 ℃ for 60min until the temperature is 25 ℃, obtaining a solid culture medium, inoculating ganoderma lucidum seeds into the solid culture medium in an ultra-clean workbench, enabling the inoculation amount to be 5%, placing the solid culture medium in a culture room, enabling the temperature to be 28 ℃ and the humidity to be 70%, culturing for 10 days, and forming ganoderma lucidum aerial hyphae which are white, fluffy and radial in edge, screening ganoderma lucidum aerial hyphae which are white in color, free of abnormal color change, uniform and thick, and capable of growing, and screening ganoderma lucidum aerial hyphae which are thick in a form and have the diameter of not smaller than 1 mu m by adopting a scanning electron microscope observation method, as shown in a graph 2.
Step 2, drying the ganoderma aerial hyphae with the induction chip:
Placing a round induction chip with a clean surface and a diameter of 1cm and a thickness of 0.3mm in the ganoderma lucidum aerial hypha grown in the solid culture medium obtained in the step 1, culturing the induction chip for 2 days as shown in figure 4, surrounding and interweaving the induction chip surface with ganoderma lucidum aerial hypha to cover the induction chip, obtaining ganoderma lucidum aerial hypha with the induction chip as shown in figure 5, stripping the ganoderma lucidum aerial hypha with the induction chip from the solid culture medium, placing the ganoderma lucidum aerial hypha into a constant-temperature blast drying oven, drying at 45 ℃ for 6 hours to obtain the dried ganoderma lucidum aerial hypha with the induction chip, and screening the moisture content of the dried ganoderma lucidum aerial hypha with the induction chip by adopting a decompression drying method to be no more than 4%.
Step3, preparing ganoderma lucidum fungus fibers:
Step 3, the dried ganoderma aerial hyphae with induction chips obtained in the step 2 can be seen as two parts, wherein one part is an induction chip with ganoderma aerial hyphae covered on the surface, the other part is the dried ganoderma aerial hyphae, the induction chip with ganoderma aerial hyphae covered on the surface is firstly taken out for standby, then 120g of the dried ganoderma aerial hyphae are put into a pulping machine, purified water is added to obtain ganoderma aerial hyphae suspension, the concentration of the ganoderma aerial hyphae in the ganoderma aerial hyphae suspension is 60g/100mL, the clearance distance of a grinding disc is set to be 1mm, the rotor power of a fly cutter is 1.1kW, the rotating speed of a fly cutter is 1470r/min, the ganoderma aerial hyphae are ground for 5min to obtain ganoderma fungus fiber liquid, the ganoderma fungus fiber liquid is put into a water bath pot with 65 ℃ and stirred for 120min at the rotating speed of 200r/min, then the ganoderma fungus fiber liquid is put into a centrifugal machine, the centrifugal sediment is washed with purified water for 15min at the temperature of 0r/min, the centrifugal sediment is centrifuged for 15min at the temperature of 4500r/min, and the obtained sediment is not more than 20 mu m, and the ganoderma lucidum fiber is not subjected to a fluorescent light microscopy with the fluorescent light of 20 mu m, and the fluorescent light microscopy fiber is not shown in the graph, and the fluorescent light is not shown, and the fluorescent light microscopy fiber is not shown, but is not shown.
Step4, preparing ganoderma lucidum fungus fiber paper pulp:
Preparing a ganoderma lucidum fiber suspension with the concentration of 30g/100mL by using 360mL of purified water to 108g ganoderma lucidum fiber obtained in the step 3, adding sodium carboxymethyl cellulose into the ganoderma lucidum fiber suspension to obtain a suspension 1, enabling the concentration of sodium carboxymethyl cellulose in the suspension 1 to be 0.2g/100mL, stirring the suspension 1 at the temperature of 65 ℃ for 30min at the speed of 150r/min by using a magnetic stirrer, then adding cationic starch into the suspension 1 to obtain a suspension 2, enabling the concentration of the cationic starch in the suspension 2 to be 0.5g/100mL, stirring the suspension 2 at the temperature of 65 ℃ for 45min by using a magnetic stirrer, obtaining ganoderma lucidum fiber pulp, screening the surface charge of the ganoderma lucidum fiber pulp by using a potentiometer to be not less than 8mV, and screening the average particle size of the ganoderma lucidum fiber pulp by using a particle size meter to be not less than 1mm.
Step 5, preparing ganoderma lucidum fungus fiber-based induction chip embedded paper:
360mL of ganoderma lucidum fungus fiber pulp obtained in the step 4, 7200mL of purified water is used for preparing ganoderma lucidum fungus pulp suspension, the concentration of ganoderma lucidum fungus fibers in the ganoderma lucidum fungus pulp suspension is 1.5 g/100mL, the ganoderma lucidum fungus pulp suspension is divided into two parts with the volume ratio of 1:1, and a vacuum suction filter is adopted to carry out suction filtration on the two parts of ganoderma lucidum fungus pulp suspension for two times, so that two ganoderma lucidum fungus fiber filter cakes are obtained; spreading one of the Ganoderma lucidum fiber filter cakes in a square mold with a side length of 20cm, trowelling the surface of the Ganoderma lucidum fiber filter cake by a scraper, then placing a standby induction chip with the surface covered with Ganoderma lucidum aerial hyphae mentioned in the step 3 in the center of the Ganoderma lucidum fiber filter cake, placing the other Ganoderma lucidum fiber filter cake on the first trowelled Ganoderma lucidum fiber filter cake, trowelling the surface of the Ganoderma lucidum fiber filter cake by the scraper, taking down the mold, placing the overlapped Ganoderma lucidum fiber filter cakes in a paper former, setting a heating power of 10kw, a drying temperature of 45 ℃ and a drying time of 12h to obtain the embedded paper of the Ganoderma lucidum fiber-based induction chip, as shown in figure 9, the contact angle of the ganoderma lucidum fiber-based induction chip embedded paper with water is not less than 79 degrees, the folding endurance tester is used for screening the induction function of the induction chip, the ganoderma lucidum fiber-based induction chip embedded paper is not influenced by 100 folds, the electronic universal tester is used for screening the tensile strength of the ganoderma lucidum fiber-based induction chip embedded paper to be not less than 180MPa, the smoothness tester is used for screening the surface smoothness of the ganoderma lucidum fiber-based induction chip embedded paper to be not less than 600s, the stiffness of the ganoderma lucidum fiber-based induction chip embedded paper to be not less than 0.1mNm is used for screening the glossiness of the ganoderma lucidum fiber-based induction chip embedded paper to be not less than 70 percent.
The preparation mechanism of the edible and medicinal fungus fiber-based sensing chip embedded paper comprises that the edible and medicinal fungus fiber contains a large amount of beta-glucan, wherein the beta-glucan is a polysaccharide formed by connecting glucose monomers through beta-1, 4 glycosidic bonds, and the molecular structure of the polysaccharide contains a large amount of hydroxyl (-OH) groups. Sodium carboxymethyl cellulose is a water-soluble high molecular compound, and the molecular chain of the sodium carboxymethyl cellulose is provided with carboxyl (-COOH) and hydroxyl (-OH). In the paper pulp taking the edible and medicinal fungus fiber as the main raw material, the hydroxyl groups on the beta-glucan molecules in the edible and medicinal fungus fiber and the carboxyl groups or hydroxyl groups on the carboxymethyl cellulose sodium molecules can form hydrogen bond interaction, electrostatic interaction and the like, and hydrophobic interaction can occur in hydrophobic areas in the two molecules, namely, the long-chain structure of the carboxymethyl cellulose sodium can play a role of a bridge between the edible and medicinal fungus fiber, so that the interaction between the edible and medicinal fungus fiber is further enhanced, the physical strength properties such as tensile strength, folding resistance and the like of paper are improved, and the uniformity of the embedded paper of the edible and medicinal fungus fiber-based sensing chip can be improved. In addition, in the pulp of the fiber of the edible and medicinal fungus, the surface of the fiber of the edible and medicinal fungus is usually negatively charged, while the cationic starch is positively charged. Based on the electrostatic attraction principle, the cationic starch can be adsorbed on the surfaces of the edible and medicinal fungus fibers and is filled in gaps among the edible and medicinal fungus fibers, so that the contact area among the edible and medicinal fungus fibers is increased, the combination among the edible and medicinal fungus fibers is promoted, meanwhile, hydroxyl groups on a molecular chain of the cationic starch can also form hydrogen bonds with hydroxyl groups on the surfaces of the edible and medicinal fungus fibers, the combination among the edible and medicinal fungus fibers is enhanced, and the strength of the edible and medicinal fungus fiber base paper is further improved. In the pulping process, the cationic starch is adsorbed on the surfaces of the edible and medicinal fungus fibers, so that the charge distribution on the surfaces of the edible and medicinal fungus fibers is changed, the electrostatic repulsive force between the edible and medicinal fungus fibers is reduced, the edible and medicinal fungus fibers can be more uniformly dispersed in paper pulp, the quick sedimentation of the edible and medicinal fungus fibers can be prevented to a certain extent, the suspension time of the edible and medicinal fungus fibers in the paper pulp is prolonged, the uniform distribution of the edible and medicinal fungus fibers is facilitated, and the uniformity of paper is further improved.
Through the specific implementation steps, the ganoderma lucidum fiber-based sensing chip embedded paper is processed, and the apparent form is shown in figure 6.
According to another aspect of the invention, a paper prepared by the method for embedding the ganoderma lucidum fiber-based sensing chip into paper is also provided.
According to another aspect of the invention, the invention also provides application of the ganoderma lucidum fiber-based sensing chip embedded paper as packaging paper.
Example 2 of the present invention:
The main difference from example 1 is that in step 1, the composition of the solid culture medium material is different from that of the wood chip 888g, the bran 264g, the gypsum 12 g and the sugar 12 g, namely, the wood chip 74%, the bran 22%, the gypsum 1%, the sugar 1% and the other steps are the same as those of example 1.
Example 3 of the present invention:
The main difference from example 1 is that in step 1, the composition of the solid culture medium material is different from that of wood dust 864 g, bran 264g, gypsum 24 g and sugar 24 g, namely, wood dust 72%, bran 26%, gypsum 1%, sugar 1% and the other steps are the same as those of example 1.
Example 4 of the present invention:
The main difference with the example 1 is that in the step 1, the inoculation amount of the ganoderma lucidum strain is 7%, and the other steps are the same as those of the example 1.
Example 5 of the present invention:
The main difference with the example 1 is that in the step 1, the inoculation amount of the ganoderma lucidum strain is 9%, and the other steps are the same as those of the example 1.
And (3) effect test:
measuring parameters of the ganoderma lucidum fungus fiber-based induction chip embedded paper:
Each parameter of the ganoderma lucidum fiber-based sensing chip embedded paper prepared in examples 1 to 5 was measured, a contact angle between the ganoderma lucidum fiber-based sensing chip embedded paper and water was measured by a contact angle measuring instrument, the influence of 100 folds of the ganoderma lucidum fiber-based sensing chip embedded paper on the sensing function of the sensing chip was measured by a folding endurance measuring instrument, the tensile strength of the ganoderma lucidum fiber-based sensing chip embedded paper was measured by an electronic universal tester, the surface smoothness of the ganoderma lucidum fiber-based sensing chip embedded paper was measured by a smoothness measuring instrument, the stiffness of the ganoderma lucidum fiber-based sensing chip embedded paper was measured by a stiffness measuring instrument, and the gloss of the ganoderma lucidum fiber-based sensing chip embedded paper was measured by a gloss measuring instrument, and specific values are shown in table 1:
TABLE 1 parameters of the ganoderma lucidum fiber-based sensor chip embedded paper prepared in examples 1-5.
As can be seen from Table 1, examples 2 and 3 are sheets with embedded glossy ganoderma fiber-based sensing chips prepared from different solid culture medium raw material ratios, and the parameters of the sheets are slightly different, wherein in example 1, the contact angle between the glossy ganoderma fiber-based sensing chips and water is not less than 79 DEG, the induction function of the sensing chips is not affected by 100 folds of the glossy ganoderma fiber-based sensing chips, the tensile strength of the glossy ganoderma fiber-based sensing chips is not less than 180MPa, the surface smoothness of the glossy ganoderma fiber-based sensing chips is not less than 600s, the stiffness of the glossy ganoderma fiber-based sensing chips is not less than 0.1mNm, and the glossiness of the glossy ganoderma fiber-based sensing chips is not less than 70%;
In comparison with example 1, examples 4 and 5 are glossy ganoderma bacteria fiber-based induction chip embedded paper prepared by different inoculation amounts of glossy ganoderma bacteria, wherein various parameters of the glossy ganoderma bacteria fiber-based induction chip embedded paper are slightly different, example 4 meets the following conditions that the contact angle of the glossy ganoderma bacteria fiber-based induction chip embedded paper with water is not less than 79 degrees, the glossy ganoderma bacteria fiber-based induction chip embedded paper can not influence the induction function of the induction chip after being folded for 100 times, the tensile strength of the glossy ganoderma bacteria fiber-based induction chip embedded paper is not less than 180MPa, the surface smoothness of the glossy ganoderma bacteria fiber-based induction chip embedded paper is not less than 600s, the stiffness of the glossy ganoderma bacteria fiber-based induction chip embedded paper is not less than 0.1mNm, and the glossiness of the glossy ganoderma bacteria fiber-based induction chip embedded paper is not less than 70%.
The steps in the method of the embodiment of the invention can be sequentially adjusted, combined and deleted according to actual needs. In the scheme of the invention, the descriptions of the embodiments are focused on, and parts, which are not detailed or described in a certain embodiment, of the embodiments can be referred to as related descriptions of other embodiments. The technical features of the technical scheme of the invention can be arbitrarily combined, and all possible combinations of the technical features in the embodiment are not described for simplifying the description, however, as long as the combinations of the technical features are not contradictory, the scope of the invention is considered.
The foregoing is merely a preferred embodiment of the present application and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present application, which are intended to be comprehended within the scope of the present application.
Claims (17)
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| NZ525521A (en) * | 2003-04-24 | 2005-12-23 | Univ Waikato | Isolation and use of white rot fungi |
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