CN119979315A

CN119979315A - An efficient detection device and method for bacterial content in a pipe column based on a shale gas production system

Info

Publication number: CN119979315A
Application number: CN202510481389.4A
Authority: CN
Inventors: 唐诗国; 葛枫; 桑宇; 周长林; 陶诗平; 赖宁; 冉立; 李鸘; 杨海; 钟海峰
Original assignee: Sichuan Shengnuo Oil And Gas Engineering Technology Service Co ltd
Current assignee: Sichuan Shengnuo Oil And Gas Engineering Technology Service Co ltd
Priority date: 2025-04-17
Filing date: 2025-04-17
Publication date: 2025-05-13
Anticipated expiration: 2045-04-17
Also published as: CN119979315B

Abstract

The invention discloses a high-efficiency detection device and method for the bacterial content in a tubular column based on a shale gas exploitation system, which relates to the technical field of detecting the bacterial content, the device comprises a machine table supported on the ground, an auxiliary detection assembly arranged on the machine table and used for collecting sample water, automatically separating bacteria in the sample water independently and automatically shaking a reagent, and a liquid adding and shading assembly positioned on the right side of the auxiliary detection assembly; the right end of the rotating shaft is provided with a biomembrane installation assembly positioned right below the straight cylinder. The invention has the advantages of reducing the working intensity of workers, greatly improving the detection efficiency of the bacterial content in the pipe column and timely changing the displacement of the variable pump.

Description

Efficient detection device and method for bacterial content in tubular column based on shale gas exploitation system

Technical Field

The invention relates to the technical field of detection of bacterial content, in particular to a high-efficiency detection device and method for bacterial content in a tubular column based on a shale gas exploitation system.

Background

The structure of a shale gas exploitation system used in a certain area is shown in fig. 1, and the shale gas exploitation system is used for exploiting underground shale gas, and comprises a shaft 1 fixedly arranged in the well, a gas exploitation tree 2 fixedly arranged on the top surface of the shaft 1, a pipe column 3 arranged in the shaft 1, the top end part of the pipe column 3 fixedly arranged on the gas exploitation tree 2, a gas-water separator 5 connected to a tail end valve 4 of the gas exploitation tree 2, a gas transmission pipeline 6 and a drainage pipeline 7 connected to the gas-water separator 5, a first stop valve 8 connected to the drainage pipeline 7, a sand remover 9 connected to the tail end opening of the first stop valve 8, a second stop valve 10 connected to the tail end opening of the sand remover 9, and a water outlet pipe 11 connected to the other end opening of the second stop valve 10, wherein the first stop valve 8 and the second stop valve 10 are in a closed state in an initial state;

The shale gas exploitation system further comprises a variable pump 12 and a sterilizing corrosion inhibitor storage tank 13 which are arranged on the ground, wherein a liquid suction port of the variable pump 12 and the sterilizing corrosion inhibitor storage tank 13 are connected with an elbow pipe 14 at a liquid discharge port of the variable pump 12, the other end of the elbow pipe 14 penetrates through the side wall of the shaft 1 and extends under the pipe column 3, and an atomization ball head 15 is connected to the extending end of the elbow pipe 14.

The method for exploiting the underground shale gas by the shale gas exploitation system comprises the following steps:

S1, turning on a variable pump 12, pumping out the sterilizing corrosion inhibitor in a sterilizing corrosion inhibitor storage tank 13 by the variable pump 12, sequentially passing through the variable pump 12, an elbow pipe 14 and an atomization ball 15 under the pumping pressure, and finally spraying out from holes on the atomization ball 15, wherein the sprayed sterilizing corrosion inhibitor enters a pipe column 3;

S2, opening a tail end valve 4 and a gas-water separator 5 of a gas production tree, wherein shale gas in a production layer enters a shaft 1, then the shale gas sequentially flows through a bottom port of a pipe column 3, an inner cavity of the pipe column 3, the gas production tree 2 and the tail end valve 4 and finally enters the gas-water separator 5 under the pressure, and the flow direction of the shale gas is shown as an arrow in FIG. 1, wherein after the shale gas enters the pipe column 3, the shale gas is mixed with a sterilization corrosion inhibitor sprayed into the pipe column 3, and the sterilization corrosion inhibitor kills bacteria entrained in the shale gas, so that the phenomenon that the pipe column 3 is perforated due to corrosion of bacteria (including iron bacteria, saprophyte and sulfate reducing bacteria) is effectively avoided, and the pipe column 3 is protected;

s3, separating water entrained in shale gas by a gas-water separator 5, and conveying the separated shale gas without water from a gas pipeline 6 to a rear section, wherein the separated water enters a drainage pipeline 7, and the drainage pipeline 7 discharges the water into sewage treatment equipment;

S4, after shale gas is mined for a period of time, workers need to detect the content of bacteria in the pipe column 3 regularly, and the workers change the discharge capacity of the variable pump 12 according to the detected content of the bacteria, so that the sterilization corrosion inhibitor is accurately filled in the pipe column 3, the pipe column 3 is protected, the consumption of the sterilization corrosion inhibitor is saved, and the method for detecting the bacterial content in the pipe column 3 regularly by the workers is as follows:

S41, sampling sample water, namely placing a sampling container 16 under a water outlet pipe 11 of a shale gas exploitation system by workers, as shown in fig. 2, opening a first stop valve 8, shunting water in a drainage pipeline 7 into a sand remover 9 through the first stop valve 8, closing the first stop valve 8, removing sand and greasy dirt in the water by the sand remover 9, after the sand remover 9 is stationary for a period of time, purifying the water by the sand remover 9, opening a second stop valve 10, and enabling the purified water in the sand remover 9 to flow into the sampling container 16 through the second stop valve 10 and the water outlet pipe 11 in sequence, thereby completing sampling of the sample water;

s42, taking out a filter container shown in FIG. 3 by a worker, wherein the filter container comprises a container body 17 and an end cover 18 connected to the bottom of the container body 17, a biological film 19 is fixedly arranged between the end cover 18 and the container body 17, and a through groove 20 is formed in the middle of the end cover 18;

S43, pouring the sample water in the sampling container 16 into the container body 17 of the filtering container by workers, enabling water molecules of the sample water to sequentially pass through the biological membrane 19 and the through groove 20 and finally fall on the ground, wherein the flowing direction of the water molecules is shown by an arrow in FIG. 4, and bacteria in the sample water are intercepted on the top surface of the biological membrane 19 because the water molecules cannot pass through the biological membrane 19, so that the bacteria in the sample water are separated, and at the moment, the top surface of the biological membrane 19 is covered with a layer of bacteria;

S44, a worker inserts the blocking head 21 into the through groove 20 of the end cover 18 to block the through groove 20 as shown in fig. 5, then sequentially adds a lysis reagent and a stabilizer into the container body 17 of the filter container in the direction shown by solid arrows in fig. 6, and after the addition, the worker shakes the container body 17 to mix the lysis reagent, the stabilizer and bacteria, wherein the lysis reagent is used for destroying the bacteria to release ATP (adenosine triphosphate) in the bacteria, and the stabilizer is used for stabilizing the ATP to prevent ATP degradation;

s45, a worker adds a certain amount of luciferase reagent into the container body 17 of the filter container, and the luciferase reagent reacts with ATP to generate fluorescent substances, and the fluorescent substances emit light;

S46, a worker shields the top end opening of the container body 17 by using the shading cover plate 22, as shown in fig. 7, so as to prevent external natural light from entering the container body 17 to avoid the detection precision of the photometer, then stretches the photosensitive probe 24 of the photometer 23 into the shading cover plate 22, as shown in fig. 8, the photosensitive probe 24 detects the intensity of light in the container body 17, and the content of bacteria in the pipe column 3 is finally detected because the light intensity is proportional to the content of the bacteria;

s47, pouring fluorescent substances and residual reagents in the container body 17 of the filter container into a designated waste liquid tank by workers after detection is finished so as to prepare for the next detection;

S5, when the detected bacterial content is higher than the specified content, the amount of bacteria entering the tubular column 3 is increased, at the moment, the discharge capacity of the variable pump 12 is increased, the amount of the sterilization corrosion inhibitor sprayed into the tubular column 3 is increased, so that the tubular column 3 is prevented from being corroded by bacteria, and when the detected bacterial content is lower than the specified content, the amount of bacteria entering the tubular column 3 is decreased, at the moment, the discharge capacity of the variable pump 12 is decreased, the amount of the sterilization corrosion inhibitor sprayed into the tubular column 3 is reduced, and therefore the use amount of the sterilization corrosion inhibitor is saved.

However, in step S4, although the worker can detect the content of bacteria, in actual operation, the following technical drawbacks still exist:

I. In step S43, a worker is required to manually pour the sample water in the sampling container 16 into the container body 17 of the filter container to separate bacteria in the sample water, in step S44, a worker is required to manually plug the through groove 20 of the end cover 18 by the plug 21, in step S44, the worker is required to shake the container body 17 to mix the lysis reagent, the stabilizer and the bacteria, in steps S44-S45, the worker sequentially adds the lysis reagent, the stabilizer and the luciferase reagent into the container body 17 of the filter container to react and generate fluorescent substances, in step S46, the worker is required to cover the top end opening of the container body 17 of the filter container by the light shielding cover 22, and the worker is required to extend the photosensitive probe 24 of the photometer 23 into the light shielding cover 22 to detect the light intensity by the photometer 23, so that the content of the bacteria in the tubular column 3 can be finally detected.

In the whole detection process, manual operation is performed by manpower, and detection is discontinuous, so that the working intensity of workers is increased, and long time is required to complete detection of the bacterial content once, thereby reducing the detection efficiency of the bacterial content in the pipe column 3, further leading to incapability of timely changing the displacement of the variable pump 12, and still achieving the purposes of protecting the pipe column 3 and saving the use amount of the sterilization corrosion inhibitor.

II. In step S47, after the detection is completed, the worker is required to turn the filter container to a position right above the waste liquid tank, and then pour all the fluorescent material and the residual reagent in the container body 17 into the waste liquid tank, and the whole pouring process is manually operated, so that the working strength of the worker is increased.

Therefore, there is a need for a high-efficiency detection device and method that can reduce the work intensity of workers, greatly improve the detection efficiency of the bacterial content in the pipe string 3, and timely change the displacement of the variable pump.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides the high-efficiency detection device and the method for the bacterial content in the pipe column based on the shale gas exploitation system, which are used for reducing the working intensity of workers, greatly improving the detection efficiency of the bacterial content in the pipe column and timely changing the displacement of a variable pump.

The invention aims at realizing the technical scheme that the high-efficiency detection device for the bacterial content in the pipe column based on the shale gas exploitation system comprises a machine table supported on the ground, an auxiliary detection assembly which is arranged on the machine table and used for collecting sample water, automatically separating bacteria in the sample water and automatically shaking a reagent, and a liquid adding and shading assembly which is positioned on the right side of the auxiliary detection assembly;

The auxiliary detection assembly comprises a driving assembly fixedly arranged on the machine table, an L plate is fixedly arranged at the top of the driving assembly, a feeding oil cylinder is fixedly arranged on the L plate, a piston rod of the feeding oil cylinder penetrates through the L plate leftwards, a movable plate is fixedly arranged on the extending end of the feeding oil cylinder, a straight cylinder is hinged to the left side of the movable plate through a hinge seat, a reciprocating oil cylinder is fixedly arranged on the right side of the movable plate, a piston rod of the reciprocating oil cylinder penetrates through the movable plate leftwards, a connecting rod is hinged to the extending end of the reciprocating oil cylinder, and the other end of the connecting rod is hinged to the straight cylinder;

A main oil cylinder is fixedly arranged on the left side wall of the straight cylinder, a lifting plate is fixedly arranged on a piston rod of the main oil cylinder, a secondary oil cylinder is fixedly arranged on the top surface of the lifting plate, the piston rod of the secondary oil cylinder downwards penetrates through the lifting plate, a rack is fixedly arranged on the extending end of the lifting plate, a connecting plate is fixedly arranged on the bottom surface of the lifting plate, a rotating shaft is rotatably arranged at the lower end part of the connecting plate, a gear is arranged at the left end part of the rotating shaft and is meshed with the rack, a biological film installation assembly positioned right below the straight cylinder is arranged at the right end part of the rotating shaft, the biological film installation assembly comprises a lower annular clamping plate, biological films and an upper annular clamping plate which are sequentially stacked from bottom to top, a plurality of locking screws are connected at the outer edges of the upper annular clamping plate and the lower annular clamping plate, the outer edge of the biological film is fixedly clamped between the upper annular clamping plate and the lower annular clamping plate under the threaded connection force of the locking screws, the top surface of the upper annular clamping plate is pressed on a sealing ring positioned on the bottom surface of the straight cylinder, and the left side wall of the lower annular clamping plate is welded on the right end part of the rotating shaft;

The right side wall of the straight cylinder is fixedly provided with a fixed seat, the top surface of the fixed seat is fixedly provided with a hydraulic motor, a rotating shaft of the hydraulic motor downwards penetrates through the fixed seat, the extending end is fixedly provided with a vertical oil cylinder, the acting end of a piston rod of the vertical oil cylinder is fixedly provided with a lifting plate extending leftwards, the top surface of the lifting plate is fixedly provided with a plug, the plug seals a central hole of the lower annular clamping plate, and the bottom surface of the biological film is supported.

The driving assembly comprises a driving motor fixedly arranged on the machine table, a vertically arranged jacking cylinder is fixedly arranged on an output shaft of the driving motor, and the L plate is fixedly arranged on an acting end of a piston rod of the jacking cylinder.

And a liquid level sensor is fixedly arranged in the straight cylinder and positioned on the left side wall of the straight cylinder.

Center holes are formed in the middle of the upper annular clamping plate and the middle of the lower annular clamping plate of the biological film installation assembly, and the biological film separates the upper annular clamping plate from the lower annular clamping plate.

The liquid feeding and shading component comprises a support fixedly arranged on the machine table, a cracking reagent storage tank, a stabilizer storage tank and a luciferase reagent storage tank are fixedly arranged on a transverse plate of the support, electromagnetic valves are connected to bottom end openings of the cracking reagent storage tank, the stabilizer storage tank and the luciferase reagent storage tank, a shading barrel is fixedly arranged on the transverse plate of the support, the shading barrel is located on the right side of the luciferase reagent storage tank, the top of the shading barrel is closed, a photometer is fixedly arranged in the closed end, and a photosensitive probe of the photometer is arranged downwards.

A connecting frame is fixedly arranged between the closed end of the shading cylinder and the transverse plate of the bracket.

The table top of the machine table is provided with a water receiving tank and a waste liquid tank, the water receiving tank is positioned right below the straight cylinder, and the waste liquid tank is positioned right below the shading cylinder.

The high-efficiency detection device further comprises a controller, and the driving motor, the hydraulic motor, the jacking oil cylinder, the vertical oil cylinder, the reciprocating oil cylinder, the main oil cylinder, the auxiliary oil cylinder, the liquid level sensor and the photometer are electrically connected with the controller through signal wires.

A high-efficiency detection method for the bacterial content in a tubular column based on a shale gas exploitation system comprises the following steps:

S1, sampling sample water, wherein the specific operation steps are as follows:

S11, placing a machine table of the detection device on the ground, and ensuring that a straight barrel of the detection device is positioned right below a water outlet pipe of a shale gas exploitation system;

S12, opening a first stop valve, shunting water in the drainage pipeline into the sand remover through the first stop valve, and then closing the first stop valve;

s13, removing sand and greasy dirt in water by the sand remover, after standing for 65min, cleaning the water by the sand remover, then opening a second stop valve, sequentially passing the cleaned water in the sand remover through the second stop valve and a water outlet pipe, and finally flowing into a straight cylinder, when the water just reaches a liquid level sensor, sending an electric signal to a controller by the liquid level sensor, and immediately closing the second stop valve by a worker after receiving the electric signal, wherein the straight cylinder is filled with sample water, so that the sampling of the sample water is completed;

S2, separating bacteria in sample water, wherein the specific operation steps are as follows:

S21, controlling a piston rod of a jacking cylinder of the auxiliary detection assembly to retract downwards, wherein the piston rod drives an L plate to move downwards, the L plate drives a feeding cylinder, a movable plate, a straight cylinder and a biological film installation assembly to move downwards synchronously, and when the piston rod of the jacking cylinder is completely retracted, the straight cylinder is far away from a water outlet pipe and is close to a water receiving tank;

S22, controlling a piston rod of a vertical oil cylinder of the auxiliary detection assembly to extend downwards, driving a lifting plate to move downwards by the piston rod, and driving a plug to move downwards by the lifting plate, wherein the plug just exits from a central hole of a lower annular clamping plate of the biological film installation assembly after the piston rod of the vertical oil cylinder extends completely;

S23, controlling a rotating shaft of a hydraulic motor of the auxiliary detection assembly to rotate, wherein the rotating shaft drives the vertical oil cylinder, the lifting plate and the blockage to synchronously rotate, when the blockage rotates by 90 degrees, the controller controls the hydraulic motor to be closed, at the moment, water molecules of sample water in the straight cylinder sequentially pass through a biomembrane and a central hole of the lower annular clamping plate and finally fall into the water receiving tank, bacteria in the sample water are intercepted on the top surface of the biomembrane due to incapability of passing through the biomembrane, and therefore the bacteria in the sample water are finally separated, and at the moment, the top surface of the biomembrane is covered with a layer of bacteria;

s3, controlling the rotating shaft of the hydraulic motor to reversely rotate so as to enable the blockage to move to the position right below the central hole of the lower annular clamping plate, and then controlling the piston rod of the vertical oil cylinder to retract upwards so as to enable the blockage to be plugged into the central hole of the lower annular clamping plate again and enable the biological membrane to be blocked;

S4, reacting bacteria on the surface of the biological film into fluorescent substances, wherein the specific operation steps are as follows:

s41, controlling an output shaft of a driving motor to rotate, wherein the output shaft drives a jacking oil cylinder, an L plate, a feeding oil cylinder, a movable plate, a straight cylinder and a biological film installation assembly to synchronously rotate, and after the straight cylinder rotates 180 degrees, a controller controls the driving motor to be closed, and at the moment, the straight cylinder just moves to a liquid adding station of a liquid adding and shading assembly, namely, directly below a cracking reagent storage tank, a stabilizer storage tank and a luciferase reagent storage tank of the straight cylinder;

S42, controlling a piston rod of a jacking cylinder of the auxiliary detection assembly to extend upwards, wherein the piston rod drives an L plate to move upwards, the L plate drives a feeding cylinder, a movable plate, a straight cylinder and a biological film installation assembly to move upwards synchronously, and when the piston rod of the jacking cylinder extends completely, the top end opening of the straight cylinder is close to a cracking reagent storage tank, a stabilizer storage tank and a luciferase reagent storage tank;

S43, controlling the electromagnetic valve of the cracking reagent storage tank to start, enabling the cracking reagent in the cracking reagent storage tank to enter the straight cylinder through the electromagnetic valve, submerging bacteria on the surface of the biological film, closing the electromagnetic valve of the cracking reagent storage tank when the bacteria are added to a set time point, and then controlling the electromagnetic valve of the stabilizer storage tank to start, enabling the stabilizer in the stabilizer storage tank to enter the straight cylinder through the electromagnetic valve, and closing the electromagnetic valve of the stabilizer storage tank when the bacteria are added to the set time point;

S44, controlling a piston rod of a reciprocating cylinder of the auxiliary detection assembly to reciprocate, wherein the piston rod drives a connecting rod to reciprocate left and right, and the connecting rod drives a straight cylinder to reciprocate around a hinge seat, so that a cracking reagent, a stabilizer and bacteria are mixed, in the mixing process, the cracking reagent damages the bacteria to release ATP in the bacteria, and the stabilizer stabilizes the ATP to prevent ATP degradation;

s45, controlling the solenoid valve of the luciferase reagent storage tank to start, enabling the luciferase reagent in the luciferase reagent storage tank to enter the straight cylinder through the solenoid valve, closing the solenoid valve of the luciferase reagent storage tank when the luciferase reagent is added to a set time point, and reacting the luciferase reagent with ATP to generate luminescent fluorescent materials at the moment, so that all bacteria on the surface of the biological film are reacted to the fluorescent materials finally;

s5, detecting the light intensity of light emitted by fluorescent materials in the straight cylinder, wherein the specific operation steps are as follows:

S51, controlling a piston rod of a jacking cylinder of the auxiliary detection assembly to retract downwards, wherein the piston rod drives an L plate to move downwards, and the L plate drives a feeding cylinder, a movable plate, a straight cylinder and a biological film installation assembly to move downwards synchronously;

S52, controlling a piston rod of the feeding oil cylinder to extend rightwards, driving the movable plate to move rightwards by the piston rod, driving the straight cylinder and the biological film installation assembly to synchronously move rightwards by the movable plate, and enabling the straight cylinder to move right below a shading cylinder of the liquid adding and shading assembly after the piston rod of the feeding oil cylinder extends completely;

S53, controlling a piston rod of a jacking cylinder to extend upwards, driving an L plate to move upwards, driving a straight cylinder to move upwards by the L plate, enabling a top end opening of the straight cylinder to be just embedded into a shading cylinder after the piston rod of the jacking cylinder extends completely, shading the top end opening of the straight cylinder at the moment to prevent external natural light from entering a container body, simultaneously enabling a photosensitive probe of a photometer to enter the straight cylinder, detecting the light intensity of light emitted by fluorescent materials by the photosensitive probe, converting the light intensity into an electric signal by the photosensitive probe, transmitting the electric signal to a controller by the photosensitive probe, and calculating the content of bacteria in a pipe column by the controller according to the electric signal;

When the detected bacterial content is higher than the specified content, the number of the bacteria entering the pipe column is large, at the moment, a worker regulates the discharge capacity of the variable pump, and further increases the amount of the sterilization corrosion inhibitor sprayed into the pipe column so as to prevent the pipe column from being corroded by the bacteria, and thereby the pipe column is protected; when the detected bacterial content is lower than the specified content, the bacterial quantity entering the tubular column is small, and at the moment, a worker adjusts the discharge capacity of the variable displacement pump, so that the quantity of the sterilization corrosion inhibitor sprayed into the tubular column is reduced, and the use quantity of the sterilization corrosion inhibitor is saved;

s6, after the detection is finished, the operation steps of pouring the fluorescent material and the residual reagent in the straight cylinder into the waste liquid tank by workers are as follows:

S61, controlling a piston rod of a jacking cylinder of the auxiliary detection assembly to retract downwards, so that the straight cylinder and the biological film installation assembly synchronously move downwards;

S62, controlling a piston rod of a vertical oil cylinder of an auxiliary detection assembly to extend downwards so as to enable the blockage to just withdraw from a central hole of a lower annular clamping plate of the biological film installation assembly, then controlling a hydraulic motor to rotate, driving the vertical oil cylinder, a lifting plate and the blockage to synchronously rotate, and controlling the hydraulic motor to be closed by a controller after the blockage rotates 180 degrees, wherein at the moment, residual reagent in a straight cylinder sequentially passes through the central hole of an upper annular clamping plate, the biological film and the central hole of the lower annular clamping plate and finally falls into a waste liquid tank, so that the residual reagent is discharged into the waste liquid tank;

S63, controlling a piston rod of a main oil cylinder of the auxiliary detection assembly to extend downwards, driving a lifting plate to move downwards by the piston rod, driving a secondary oil cylinder, a rack, a connecting plate, a gear, a rotating shaft and a biological film installation assembly to move downwards synchronously by the lifting plate, and separating the biological film installation assembly from a straight cylinder just after the piston rod of the main oil cylinder extends completely;

S64, controlling a piston rod of a secondary oil cylinder of the auxiliary detection assembly to retract upwards, enabling the piston rod to drive the rack to move upwards, enabling the rack to drive the gear to rotate, enabling the gear to drive the rotating shaft to rotate, enabling the rotating shaft to drive the biological film installation assembly to rotate synchronously, enabling the biological film installation assembly to be converted into a vertical state after the piston rod of the secondary oil cylinder is completely retracted, enabling fluorescent substances on the surface of the biological film to fall into a waste liquid tank through a central hole of an upper annular clamping plate, and accordingly enabling the fluorescent substances to be poured into the waste liquid tank to prepare for secondary bacterial content.

The invention has the advantages of reducing the working intensity of workers, greatly improving the detection efficiency of the bacterial content in the pipe column and timely changing the displacement of the variable pump.

Drawings

FIG. 1 is a schematic structural diagram of a shale gas extraction system;

FIG. 2 is a schematic illustration of a worker placing a sampling vessel directly below a water outlet pipe of a shale gas recovery system;

FIG. 3 is a schematic view of the structure of a filter vessel;

FIG. 4 is a schematic illustration of a worker pouring sample water in a sampling container into the container body of a filter container;

FIG. 5 is a schematic illustration of a worker inserting a capping head into a through slot of an end cap;

FIG. 6 is a schematic illustration of a worker sequentially adding a lysing agent and a stabilizing agent to the vessel body of a filtration vessel;

FIG. 7 is a schematic view of a worker shielding the top end opening of the container body with a light shielding cover;

FIG. 8 is a schematic view of a worker extending a photosensitive probe of a photometer into a light shielding cover plate;

FIG. 9 is a schematic diagram of the structure of the present invention;

FIG. 10 is a schematic diagram of the structure of the auxiliary detecting assembly;

FIG. 11 is a schematic view in the direction A of FIG. 10;

FIG. 12 is a schematic diagram of the main section of FIG. 10;

FIG. 13 is a schematic illustration of the attachment of the biofilm mounting assembly, shaft and gear of FIG. 12;

FIG. 14 is a top view of FIG. 13;

FIG. 15 is a schematic view of a liquid feeding and shading assembly;

FIG. 16 is a schematic view of the straight barrel of the present invention directly below the outlet pipe of the shale gas recovery system;

FIG. 17 is a schematic view of the straight cylinder being spaced apart from the outlet pipe;

FIG. 18 is a schematic illustration of the plug exiting from the central bore of the lower annular clamping plate of the biofilm mounting assembly;

FIG. 19 is a schematic view of the plug rotated 90;

FIG. 20 is a schematic view of a straight barrel moving to a priming station of a priming and shading assembly;

FIG. 21 is a schematic view of the top end opening of a straight barrel adjacent to a lysis reagent reservoir, a stabilizer reservoir, and a luciferase reagent reservoir;

FIG. 22 is a schematic view of the straight barrel moving directly under the shade barrel of the liquid feeding and shade assembly;

FIG. 23 is a schematic view of the top end opening of the straight barrel being embedded into the shade barrel;

FIG. 24 is a schematic illustration of the occlusion rotated 180;

FIG. 25 is a schematic illustration of the biofilm mounting assembly separated from the cartridge;

FIG. 26 is a schematic illustration of the biofilm mounting assembly being converted to a vertical state;

In the figure:

1-well bore, 2-gas production tree, 3-pipe column, 4-end valve, 5-gas-water separator, 6-gas pipeline, 7-drainage pipeline, 8-first stop valve, 9-sand remover, 10-second stop valve, 11-water outlet pipe, 12-variable pump, 13-sterilizing corrosion inhibitor storage tank, 14-elbow pipe, 15-atomizing bulb, 16-sampling container, 17-container body, 18-end cover, 19-biological film, 20-through tank, 21-blocking head, 22-shading cover plate, 23-photometer, 24-photosensitive probe;

25-machine table, 26-auxiliary detection component, 27-liquid adding and shading component, 28-L plate, 29-feeding cylinder, 30-movable plate, 31-hinge seat, 32-straight cylinder, 33-reciprocating cylinder and 34-connecting rod;

The hydraulic lifting device comprises a main cylinder 35, a lifting plate 36, an auxiliary cylinder 37, a rack 38, a connecting plate 39, a rotating shaft 40, a gear 41, a lower annular clamping plate 42, an upper annular clamping plate 43, a hydraulic motor 44, a vertical cylinder 45, a lifting plate 46, a blocking 47, a driving motor 48 and a lifting cylinder 49;

50-bracket, 51-cracking reagent storage tank, 52-stabilizer storage tank, 53-luciferase reagent storage tank, 54-shading cylinder, 55-water receiving tank and 56-waste liquid tank.

Detailed Description

The invention is further described below with reference to the accompanying drawings, the scope of the invention not being limited to the following:

As shown in FIG. 9, the efficient detection device for the bacterial content in the pipe column based on the shale gas exploitation system comprises a machine table 25 supported on the ground, an auxiliary detection assembly 26 arranged on the machine table 25 and used for collecting sample water and automatically separating out bacteria in the sample water and automatically shaking a reagent, and a liquid adding and shading assembly 27 positioned on the right side of the auxiliary detection assembly 26 is further arranged on the machine table 25.

As shown in fig. 10-14, the auxiliary detecting assembly 26 comprises a driving assembly fixedly arranged on the machine 25, an L plate 28 is fixedly arranged at the top of the driving assembly, a feeding oil cylinder 29 is fixedly arranged on the L plate 28, a piston rod of the feeding oil cylinder 29 penetrates through the L plate 28 leftwards, a movable plate 30 is fixedly arranged on the extending end of the feeding oil cylinder, a straight cylinder 32 is hinged to the left side of the movable plate 30 through a hinge seat 31, a reciprocating oil cylinder 33 is fixedly arranged on the right side of the movable plate 30, a piston rod of the reciprocating oil cylinder 33 penetrates through the movable plate 30 leftwards, a connecting rod 34 is hinged to the extending end, the other end of the connecting rod 34 is hinged to the straight cylinder 32, and a liquid level sensor is fixedly arranged in the straight cylinder 32 and on the left side wall of the straight cylinder. The driving assembly comprises a driving motor 48 fixedly arranged on the machine table 25, a vertically arranged jacking cylinder 49 is fixedly arranged on an output shaft of the driving motor 48, and the L plate 28 is fixedly arranged on an acting end of a piston rod of the jacking cylinder 49.

As shown in fig. 10-14, a main cylinder 35 is fixed on the left side wall of the straight cylinder 32, a lifting plate 36 is fixed on a piston rod of the main cylinder 35, a secondary cylinder 37 is fixed on the top surface of the lifting plate 36, a rack 38 is fixed on the extending end of the piston rod of the secondary cylinder 37, a connecting plate 39 is fixed on the bottom surface of the lifting plate 36, a rotating shaft 40 is rotatably mounted at the lower end of the connecting plate 39, a gear 41 is mounted at the left end of the rotating shaft 40, the gear 41 is meshed with the rack 38, a biological film mounting assembly positioned right below the straight cylinder 32 is arranged at the right end of the rotating shaft 40, the biological film mounting assembly comprises a lower annular clamping plate 42, a biological film 19 and an upper annular clamping plate 43 which are sequentially stacked from bottom to top, a plurality of locking screws are connected at the outer edges of the upper annular clamping plate 43 and the lower annular clamping plate 42, the outer edges of the biological film 19 are clamped and fixed between the upper annular clamping plate 43 and the lower annular clamping plate 42 under the threaded connection force of the locking screws, the top surface of the upper annular clamping plate 43 is pressed against a sealing ring positioned on the bottom surface of the straight cylinder 32, and the right side wall of the upper side wall of the rotating shaft 40 is welded on the right side wall of the rotating shaft 40. Center holes are formed in the middle of the upper annular clamping plate 43 and the lower annular clamping plate 42 of the biological film installation assembly, and the biological film 19 separates the upper annular clamping plate 43 from the lower annular clamping plate 42.

The right side wall of the straight cylinder 32 is fixedly provided with a fixed seat, the top surface of the fixed seat is fixedly provided with a hydraulic motor 44, a rotating shaft of the hydraulic motor 44 downwards penetrates through the fixed seat, the extending end is fixedly provided with a vertical oil cylinder 45, the acting end of a piston rod of the vertical oil cylinder 45 is fixedly provided with a lifting plate 46 extending leftwards, the top surface of the lifting plate 46 is fixedly provided with a plug 47, and the plug 47 plugs a central hole of the lower annular clamping plate 42 and supports the bottom surface of the biological membrane 19.

As shown in fig. 15, the liquid adding and shading assembly 27 comprises a bracket 50 fixed on the machine 25, a cracking reagent storage tank 51, a stabilizer storage tank 52 and a luciferase reagent storage tank 53 are fixed on a transverse plate of the bracket 50, electromagnetic valves are connected to bottom end openings of the cracking reagent storage tank 51, the stabilizer storage tank 52 and the luciferase reagent storage tank 53, a shading cylinder 54 is also fixed on the transverse plate of the bracket 50, the shading cylinder 54 is positioned on the right side of the luciferase reagent storage tank 53, the top of the shading cylinder 54 is closed, a photometer 23 is fixed in the closed end, and a photosensitive probe 24 of the photometer 23 is arranged downward. A connecting frame is fixedly arranged between the closed end of the shading cylinder 54 and the transverse plate of the bracket 50.

A water receiving tank 55 and a waste liquid tank 56 are arranged on the table top of the machine table 25, the water receiving tank 55 is positioned right below the straight cylinder 32, and the waste liquid tank 56 is positioned right below the shading cylinder 54.

The high-efficiency detection device further comprises a controller, wherein the driving motor 48, the hydraulic motor 44, the lifting oil cylinder 49, the vertical oil cylinder 45, the reciprocating oil cylinder 33, the main oil cylinder 35, the auxiliary oil cylinder 37, the liquid level sensor and the photometer 23 are electrically connected with the controller through signal lines, a worker can control the starting or closing of the driving motor 48 and the hydraulic motor 44 through the controller, and meanwhile, the lifting oil cylinder 49, the vertical oil cylinder 45, the reciprocating oil cylinder 33, the main oil cylinder 35 and the piston rods of the auxiliary oil cylinder 37 can be controlled to extend or retract, so that the operation of the worker is facilitated, and the high-automation-level detection device is characterized in that the automation degree is high.

S1, sampling sample water, wherein the specific operation steps are as follows:

S11, placing the machine 25 of the detection device on the ground, and ensuring that a straight cylinder 32 of the detection device is positioned right below a water outlet pipe 11 of the shale gas exploitation system shown in FIG. 1, as shown in FIG. 16;

S12, opening a first stop valve 8, shunting water in the drainage pipeline 7 into the sand remover 9 through the first stop valve 8, and then closing the first stop valve 8;

S13, removing sand and greasy dirt in water by the sand remover 9, after standing for 65min, purifying the water by the sand remover 9, opening a second stop valve 10, sequentially passing the purified water in the sand remover 9 through the second stop valve 10 and a water outlet pipe 11, and finally flowing into a straight cylinder 32, when the water just reaches a liquid level sensor, sending an electric signal to a controller by the liquid level sensor, and immediately closing the second stop valve 10 by a worker after receiving the electric signal, wherein the straight cylinder 32 is filled with sample water, thereby completing sampling of the sample water;

S21, controlling a piston rod of a lifting cylinder 49 of the auxiliary detection assembly 26 to retract downwards, wherein the piston rod drives an L plate 28 to move downwards, the L plate 28 drives a feed cylinder 29, a movable plate 30, a straight cylinder 32 and a biological film installation assembly to move downwards synchronously, and when the piston rod of the lifting cylinder 49 is fully retracted, the straight cylinder 32 is far away from the water outlet pipe 11, as shown in FIG. 17, and is close to a water receiving groove 55;

s22, controlling a piston rod of a vertical oil cylinder 45 of the auxiliary detection assembly 26 to extend downwards, driving a lifting plate 46 to move downwards by the piston rod, driving a plug 47 to move downwards by the lifting plate 46, and just withdrawing the plug 47 from a central hole of a lower annular clamping plate 42 of the biological film installation assembly after the piston rod of the vertical oil cylinder 45 extends completely, as shown in fig. 18;

S23, controlling a rotating shaft of a hydraulic motor 44 of the auxiliary detection assembly 26 to rotate, wherein the rotating shaft drives a vertical oil cylinder 45, a lifting plate 46 and a plug 47 to synchronously rotate, when the plug 47 rotates by 90 degrees, as shown in fig. 19, the controller controls the hydraulic motor 44 to be closed, at the moment, water molecules of sample water in a straight cylinder 32 sequentially pass through a biological membrane 19 and a central hole of a lower annular clamping plate 42 and finally fall into a water receiving tank 55, the movement direction of the water molecules is shown by hollow arrows in fig. 19, bacteria in the sample water are intercepted on the top surface of the biological membrane 19 because the bacteria cannot pass through the biological membrane 19, and therefore, the separation of the bacteria in the sample water is finally realized, and at the moment, the top surface of the biological membrane 19 is covered with a layer of bacteria;

S3, controlling the rotation shaft of the hydraulic motor 44 to reversely rotate so as to enable the plug 47 to move to the position right below the central hole of the lower annular clamping plate 42, and then controlling the piston rod of the vertical oil cylinder 45 to retract upwards so as to enable the plug 47 to be plugged into the central hole of the lower annular clamping plate 42 again and enable the biological film 19 to be plugged;

S4, bacteria on the surface of the biological film 19 are reacted into fluorescent substances, and the specific operation steps are as follows:

S41, controlling an output shaft of a driving motor 48 to rotate, wherein the output shaft drives a jacking oil cylinder 49, an L plate 28, a feeding oil cylinder 29, a movable plate 30, a straight cylinder 32 and a biological film installation assembly to synchronously rotate, and after the straight cylinder 32 rotates 180 degrees, the controller controls the driving motor 48 to be closed, at the moment, the straight cylinder 32 just moves to a liquid adding station of a liquid adding and shading assembly 27, as shown in FIG. 20, namely, under a cracking reagent storage tank 51, a stabilizer storage tank 52 and a luciferase reagent storage tank 53 of the straight cylinder 32;

S42, controlling a piston rod of a lifting cylinder 49 of the auxiliary detection assembly 26 to extend upwards, wherein the piston rod drives an L plate 28 to move upwards, the L plate 28 drives a feed cylinder 29, a movable plate 30, a straight cylinder 32 and a biological film installation assembly to synchronously move upwards, and when the piston rod of the lifting cylinder 49 extends completely, the top end opening of the straight cylinder 32 is close to a cracking reagent storage tank 51, a stabilizer storage tank 52 and a luciferase reagent storage tank 53, as shown in FIG. 21;

S43, controlling the electromagnetic valve of the cracking reagent storage tank 51 to start, enabling the cracking reagent in the cracking reagent storage tank 51 to enter the straight cylinder 32 through the electromagnetic valve, submerging bacteria on the surface of the biological film 19, closing the electromagnetic valve of the cracking reagent storage tank 51 when the bacteria are added to a set time point, and then controlling the electromagnetic valve of the stabilizer storage tank 52 to start, enabling the stabilizer in the stabilizer storage tank 52 to enter the straight cylinder 32 through the electromagnetic valve, and closing the electromagnetic valve of the stabilizer storage tank 52 when the bacteria are added to the set time point;

s44, controlling a piston rod of a reciprocating cylinder 33 of the auxiliary detection assembly 26 to reciprocate, wherein the piston rod drives a connecting rod 34 to reciprocate left and right, the connecting rod 34 drives a straight cylinder 32 to reciprocate around a hinge seat 31, the shaking direction of the straight cylinder 32 is shown by an arrow in FIG. 21, so that a cracking reagent, a stabilizer and bacteria are mixed, and in the mixing process, the cracking reagent damages the bacteria to release ATP in the bacteria, and the stabilizer stabilizes the ATP to prevent ATP degradation;

S45, controlling the solenoid valve of the luciferase reagent storage tank 53 to start, enabling the luciferase reagent in the luciferase reagent storage tank 53 to enter the straight cylinder 32 through the solenoid valve, closing the solenoid valve of the luciferase reagent storage tank 53 when the luciferase reagent is added to a set time point, and enabling the luciferase reagent to react with ATP to generate luminescent fluorescent substances at the moment, so that all bacteria on the surface of the biological film 19 are finally reacted to the fluorescent substances;

S5, detecting the light intensity of light emitted by fluorescent materials in the straight tube 32, wherein the specific operation steps are as follows:

S51, controlling a piston rod of a jacking cylinder 49 of the auxiliary detection assembly 26 to retract downwards, wherein the piston rod drives an L plate 28 to move downwards, and the L plate 28 drives a feeding cylinder 29, a movable plate 30, a straight cylinder 32 and a biological film installation assembly to move downwards synchronously;

s52, controlling a piston rod of the feeding oil cylinder 29 to extend rightwards, driving the movable plate 30 to move rightwards by the piston rod, driving the straight cylinder 32 and the biological film installation assembly to synchronously move rightwards by the movable plate 30, and enabling the straight cylinder 32 to move right below a shading cylinder 54 of the liquid adding and shading assembly 27 after the piston rod of the feeding oil cylinder 29 extends completely, as shown in fig. 22;

S53, controlling a piston rod of the jacking cylinder 49 to extend upwards, driving the L plate 28 to move upwards by the piston rod, driving the straight cylinder 32 to move upwards by the L plate 28, and after the piston rod of the jacking cylinder 49 extends completely, embedding a top end opening of the straight cylinder 32 into the shading cylinder 54 just as shown in FIG. 23, wherein the shading cylinder 54 shields the top end opening of the straight cylinder 32 to prevent external natural light from entering the container body 17, simultaneously, a photosensitive probe 24 of the photometer 23 enters the straight cylinder 32, the photosensitive probe 24 detects the light intensity of light emitted by fluorescent substances, the photosensitive probe 24 converts the light intensity into an electric signal, then the electric signal is transmitted to a controller by the photosensitive probe 24, and the controller calculates the bacterial content in the pipe column 3 according to the electric signal;

When the detected bacterial content is higher than the specified content, the amount of bacteria entering the tubular column 3 is increased, at the moment, the discharge capacity of the variable pump 12 is regulated by a worker, and then the amount of the sterilization corrosion inhibitor sprayed into the tubular column 3 is increased, so that the tubular column 3 is prevented from being corroded by bacteria, and the tubular column 3 is protected, when the detected bacterial content is lower than the specified content, the amount of bacteria entering the tubular column 3 is reduced, at the moment, the discharge capacity of the variable pump 12 is regulated by the worker, and then the amount of the sterilization corrosion inhibitor sprayed into the tubular column 3 is reduced, so that the use amount of the sterilization corrosion inhibitor is saved.

In step S4, the detection device automatically adds a lysis reagent, a stabilizer and a luciferase reagent into the straight tube 32 only through the linkage coordination of the auxiliary detection component 26 and the liquid adding and shading component 27, and can automatically shake the straight tube 32 to mix the lysis reagent, the stabilizer and bacteria and release ATP in the bacteria, and in step S5, the detection device automatically sleeves the shading tube 54 on the top port of the straight tube 32 only through the linkage coordination of the auxiliary detection component 26 and the liquid adding and shading component 27, and stretches the photosensitive probe 24 of the photometer 23 into the straight tube 32 to detect the light intensity and further detect the content of the bacteria in the tubular column 3;

As can be seen from the above, in the detection device, compared with the detection method shown in FIG. 2 to FIG. 8, the bacteria in the sample water can be separated independently without manually pouring the sample water in the sampling container 16 into the container body 17 of the filter container, without shaking the container body 17 by a worker to mix the lysis reagent, the stabilizer and the bacteria, without sequentially adding the lysis reagent, the stabilizer and the luciferase reagent into the container body 17 of the filter container by a worker to react and generate fluorescent substances, without shielding the top end opening of the container body 17 of the filter container by a worker with the light shielding cover plate 22, and without extending the photosensitive probe 24 of the photometer 23 into the light shielding cover plate 22 by a worker, and the light intensity can be detected by the photometer 23.

The detection device realizes automatic continuous detection, not only greatly lightens the working intensity of workers, but also realizes the detection of the bacterial content once in a short time, thereby greatly improving the detection efficiency of the bacterial content in the tubular column 3, and further changing the displacement of the variable pump 12 in time, protecting the tubular column 3 and saving the use amount of the sterilization corrosion inhibitor.

S6, after the detection is finished, the operation steps of pouring the fluorescent material and the residual reagent in the straight cylinder 32 into the waste liquid tank 56 by workers are as follows:

s61, controlling a piston rod of the lifting cylinder 49 of the auxiliary detection assembly 26 to retract downwards, so that the straight cylinder 32 and the biological film installation assembly synchronously move downwards;

S62, controlling a piston rod of a vertical oil cylinder 45 of the auxiliary detection assembly 26 to extend downwards so as to enable a plug 47 to just withdraw from a central hole of a lower annular clamping plate 42 of the biological film installation assembly, then controlling a hydraulic motor 44 to rotate, driving the vertical oil cylinder 45, a lifting plate 46 and the plug 47 to synchronously rotate, after the plug 47 rotates 180 degrees, controlling the hydraulic motor 44 to be closed by a controller, at the moment, sequentially passing through the central hole of an upper annular clamping plate 43, a biological film 19 and the central hole of the lower annular clamping plate 42 by residual reagent in a straight cylinder 32, and finally falling into a waste liquid tank 56, wherein the movement direction of the residual reagent is shown by an arrow in FIG. 24, so that the residual reagent is discharged into the waste liquid tank 56;

S63, controlling a piston rod of a main oil cylinder 35 of the auxiliary detection assembly 26 to extend downwards, driving a lifting plate 36 to move downwards by the piston rod, and driving a secondary oil cylinder 37, a rack 38, a connecting plate 39, a gear 41, a rotating shaft 40 and a biological film installation assembly to move downwards synchronously by the lifting plate 36, wherein when the piston rod of the main oil cylinder 35 extends completely, the biological film installation assembly is just separated from a straight cylinder 32, as shown in FIG. 25;

S64, the piston rod of the auxiliary oil cylinder 37 of the auxiliary detection assembly 26 is controlled to retract upwards, the piston rod drives the rack 38 to move upwards, the rack 38 drives the gear 41 to rotate, the gear 41 drives the rotating shaft 40 to rotate, the rotating shaft 40 drives the biological film installation assembly to rotate synchronously, when the piston rod of the auxiliary oil cylinder 37 is completely retracted, the biological film installation assembly is converted into a vertical state, as shown in FIG. 26, at the moment, fluorescent substances on the surface of the biological film 19 fall into the waste liquid tank 56 through the central hole of the upper annular clamping plate 43, so that the fluorescent substances are dumped into the waste liquid tank 56 to prepare for the second bacterial content.

As can be seen from step S6, the detection device only needs to control the piston rod of the vertical cylinder 45 to extend downwards so that the plug 47 just exits from the central hole of the lower annular clamping plate 42 of the biological film installation assembly, thereby realizing that the residual reagent in the straight cylinder 32 is discharged into the waste liquid tank 56, and then controls the linkage fit of the main cylinder 35 and the auxiliary cylinder 37, so that the biological film installation assembly can be converted into a vertical state, thereby realizing that the fluorescent substances on the surface of the biological film 19 are poured into the waste liquid tank 56. Therefore, the fluorescent material and the residual reagent in the straight cylinder 32 are automatically poured into the waste liquid tank 56 by the detection device without manual pouring, so that the working intensity of workers is greatly reduced.

Claims

1. The efficient detection device for the bacterial content in the pipe column based on the shale gas exploitation system is characterized by comprising a machine table (25) supported on the ground, an auxiliary detection assembly (26) which is arranged on the machine table (25) and is used for collecting sample water, automatically separating out bacteria in the sample water and automatically shaking a reagent, and a liquid adding and shading assembly (27) which is positioned on the right side of the auxiliary detection assembly (26) is further arranged on the machine table (25);

the auxiliary detection assembly (26) comprises a driving assembly fixedly arranged on the machine table (25), an L plate (28) is fixedly arranged at the top of the driving assembly, a feeding oil cylinder (29) is fixedly arranged on the L plate (28), a piston rod of the feeding oil cylinder (29) penetrates through the L plate (28) leftwards and a movable plate (30) is fixedly arranged on the extending end, a straight cylinder (32) is hinged to the left side of the movable plate (30) through a hinge seat (31), a reciprocating oil cylinder (33) is fixedly arranged on the right side of the movable plate (30), a piston rod of the reciprocating oil cylinder (33) penetrates through the movable plate (30) leftwards, a connecting rod (34) is hinged to the extending end, and the other end of the connecting rod (34) is hinged to the straight cylinder (32);

A main oil cylinder (35) is fixedly arranged on the left side wall of the straight cylinder (32), a lifting plate (36) is fixedly arranged on a piston rod of the main oil cylinder (35), a secondary oil cylinder (37) is fixedly arranged on the top surface of the lifting plate (36), a piston rod of the secondary oil cylinder (37) downwards penetrates through the lifting plate (36) and is fixedly provided with a rack (38) at the extending end, a connecting plate (39) is fixedly arranged on the bottom surface of the lifting plate (36), a rotating shaft (40) is rotatably arranged at the lower end part of the connecting plate (39), a gear (41) is arranged at the left end part of the rotating shaft (40), the gear (41) is meshed with the rack (38), a biological membrane installation assembly positioned right below the straight cylinder (32) is arranged at the right end part of the rotating shaft (40), the biological film installation assembly comprises a lower annular clamping plate (42), biological films (19) and an upper annular clamping plate (43) which are sequentially stacked from bottom to top, a plurality of locking screws are connected at the outer edges of the upper annular clamping plate (43) and the lower annular clamping plate (42), under the threaded connection force of the locking screw, the outer edge of the biological film (19) is clamped and fixed between the upper annular clamping plate (43) and the lower annular clamping plate (42), the top surface of the upper annular clamping plate (43) is propped against the sealing ring positioned on the bottom surface of the straight cylinder (32), and the left side wall of the lower annular clamping plate (42) is welded on the right end part of the rotating shaft (40);

The right side wall of the straight cylinder (32) is fixedly provided with a fixed seat, the top surface of the fixed seat is fixedly provided with a hydraulic motor (44), a rotating shaft of the hydraulic motor (44) downwards penetrates through the fixed seat, the extending end is fixedly provided with a vertical oil cylinder (45), the acting end of a piston rod of the vertical oil cylinder (45) is fixedly provided with a lifting plate (46) extending leftwards, the top surface of the lifting plate (46) is fixedly provided with a plug (47), the plug (47) plugs a central hole of the lower annular clamping plate (42), and the bottom surface of the biological film (19) is supported.

2. The efficient detection device for the bacterial content in the pipe column based on the shale gas exploitation system according to claim 1, wherein the driving assembly comprises a driving motor (48) fixedly arranged on a machine table (25), a vertically arranged jacking cylinder (49) is fixedly arranged on an output shaft of the driving motor (48), and the L plate (28) is fixedly arranged on an acting end of a piston rod of the jacking cylinder (49).

3. The efficient detection device for the bacterial content in the pipe column based on the shale gas exploitation system according to claim 2, wherein a liquid level sensor is fixedly arranged in the straight cylinder (32) and on the left side wall of the straight cylinder.

4. The efficient detection device for the bacterial content in the tubular column based on the shale gas exploitation system according to claim 3, wherein the middle parts of the upper annular clamping plate (43) and the lower annular clamping plate (42) of the biological film installation assembly are respectively provided with a central hole, and the biological film (19) separates the upper annular clamping plate (43) from the lower annular clamping plate (42).

5. The efficient detection device for the bacterial content in the pipe column based on the shale gas exploitation system according to claim 4, wherein the liquid adding and shading component (27) comprises a support (50) fixedly arranged on a machine table (25), a cracking reagent storage tank (51), a stabilizer storage tank (52) and a luciferase reagent storage tank (53) are fixedly arranged on a transverse plate of the support (50), electromagnetic valves are respectively connected to bottom end openings of the cracking reagent storage tank (51), the stabilizer storage tank (52) and the luciferase reagent storage tank (53), a shading cylinder (54) is fixedly arranged on the transverse plate of the support (50), the shading cylinder (54) is located on the right side of the luciferase reagent storage tank (53), the top of the shading cylinder (54) is closed, a photometer (23) is fixedly arranged in the closed end, and a photosensitive probe (24) of the photometer (23) is arranged downwards.

6. The efficient detection device for the bacterial content in the tubular column based on the shale gas exploitation system according to claim 5, wherein a connecting frame is fixedly arranged between the closed end of the shading cylinder (54) and the transverse plate of the bracket (50).

7. The efficient detection device for the bacterial content in the tubular column based on the shale gas exploitation system according to claim 6, wherein a water receiving tank (55) and a waste liquid tank (56) are arranged on the table top of the machine table (25), the water receiving tank (55) is located under the straight cylinder (32), and the waste liquid tank (56) is located under the shading cylinder (54).

8. The efficient detection device for the bacterial content in the pipe column based on the shale gas exploitation system according to claim 7, wherein the efficient detection device further comprises a controller, and the driving motor (48), the hydraulic motor (44), the jacking cylinder (49), the vertical cylinder (45), the reciprocating cylinder (33), the main cylinder (35), the auxiliary cylinder (37), the liquid level sensor and the photometer (23) are electrically connected with the controller through signal wires.

9. The efficient detection method for the bacterial content in the pipe column based on the shale gas exploitation system is characterized by comprising the following steps of:

S1, sampling sample water, wherein the specific operation steps are as follows:

S11, placing a machine table (25) of the detection device on the ground, and ensuring that a straight cylinder (32) of the detection device is positioned right below a water outlet pipe (11) of the shale gas exploitation system;

s12, opening a first stop valve (8), shunting water in the drainage pipeline (7) into the sand remover (9) through the first stop valve (8), and then closing the first stop valve (8);

S13, removing sand and greasy dirt in water by the sand remover (9), after standing for 65min, cleaning the water by the sand remover (9), then opening a second stop valve (10), sequentially passing the cleaned water in the sand remover (9) through the second stop valve (10) and a water outlet pipe (11), and finally flowing into a straight cylinder (32), when the water just reaches a liquid level sensor, sending an electric signal to a controller by the liquid level sensor, and immediately closing the second stop valve (10) by the worker after receiving the electric signal, wherein the straight cylinder (32) is filled with sample water, so that the sample water is sampled;

S21, controlling a piston rod of a jacking cylinder (49) of an auxiliary detection assembly (26) to retract downwards, driving an L plate (28) to move downwards by the piston rod, driving a feeding cylinder (29), a movable plate (30), a straight cylinder (32) and a biological film installation assembly to move downwards synchronously by the L plate (28), and keeping the straight cylinder (32) away from a water outlet pipe (11) and approaching a water receiving groove (55) after the piston rod of the jacking cylinder (49) is completely retracted;

S22, controlling a piston rod of a vertical oil cylinder (45) of an auxiliary detection assembly (26) to extend downwards, driving a lifting plate (46) to move downwards by the piston rod, driving a plug (47) to move downwards by the lifting plate (46), and when the piston rod of the vertical oil cylinder (45) extends completely, enabling the plug (47) to just withdraw from a central hole of a lower annular clamping plate (42) of the biological film installation assembly;

S23, controlling a rotating shaft of a hydraulic motor (44) of an auxiliary detection assembly (26) to rotate, driving a vertical oil cylinder (45), a lifting plate (46) and a plug (47) to synchronously rotate, controlling the hydraulic motor (44) to be closed by a controller after the plug (47) rotates by 90 degrees, enabling water molecules of sample water in a straight cylinder (32) to sequentially pass through a biological membrane (19) and a central hole of a lower annular clamping plate (42) and finally fall into a water receiving groove (55), and enabling bacteria in the sample water to be intercepted on the top surface of the biological membrane (19) due to the fact that the bacteria cannot pass through the biological membrane (19), so that the bacteria in the sample water are finally separated, and covering a layer of bacteria on the top surface of the biological membrane (19);

s3, controlling the rotation shaft of the hydraulic motor (44) to reversely rotate so as to enable the plug (47) to move to be right below the central hole of the lower annular clamping plate (42), and then controlling the piston rod of the vertical oil cylinder (45) to retract upwards so as to enable the plug (47) to be plugged into the central hole of the lower annular clamping plate (42) again and enable the biological film (19) to be plugged;

S4, bacteria on the surface of the biological film (19) are reacted into fluorescent substances, and the specific operation steps are as follows:

S41, controlling an output shaft of a driving motor (48) to rotate, wherein the output shaft drives a jacking oil cylinder (49), an L plate (28), a feeding oil cylinder (29), a movable plate (30), a straight cylinder (32) and a biological film installation assembly to synchronously rotate, and when the straight cylinder (32) rotates for 180 degrees, a controller controls the driving motor (48) to be closed, and at the moment, the straight cylinder (32) just moves to a liquid adding station of a liquid adding and shading assembly (27), namely, a cracking reagent storage tank (51), a stabilizer storage tank (52) and a luciferase reagent storage tank (53) of the straight cylinder (32);

s42, controlling a piston rod of a lifting cylinder (49) of an auxiliary detection assembly (26) to extend upwards, driving an L plate (28) to move upwards by the piston rod, driving a feeding cylinder (29), a movable plate (30), a straight cylinder (32) and a biological film installation assembly to synchronously move upwards by the L plate (28), and enabling a top port of the straight cylinder (32) to be close to a cracking reagent storage tank (51), a stabilizer storage tank (52) and a luciferase reagent storage tank (53) after the piston rod of the lifting cylinder (49) extends completely;

S43, controlling the electromagnetic valve of the cracking reagent storage tank (51) to start, enabling the cracking reagent in the cracking reagent storage tank (51) to enter the straight cylinder (32) through the electromagnetic valve, submerging bacteria on the surface of the biological film (19), closing the electromagnetic valve of the cracking reagent storage tank (51) when the bacteria are added to a set time point, then controlling the electromagnetic valve of the stabilizer storage tank (52) to start, enabling the stabilizer in the stabilizer storage tank (52) to enter the straight cylinder (32) through the electromagnetic valve, and closing the electromagnetic valve of the stabilizer storage tank (52) when the bacteria are added to the set time point;

s44, controlling a piston rod of a reciprocating cylinder (33) of the auxiliary detection assembly (26) to reciprocate, driving a connecting rod (34) to reciprocate left and right by the piston rod, and driving a straight cylinder (32) to reciprocate around a hinge seat (31) by the connecting rod (34), so that a cracking reagent, a stabilizer and bacteria are mixed, and in the mixing process, the cracking reagent damages bacteria to release ATP in the bacteria, and the stabilizer stabilizes ATP to prevent ATP degradation;

S45, controlling the electromagnetic valve of the luciferase reagent storage tank (53) to start, enabling the luciferase reagent in the luciferase reagent storage tank (53) to enter the straight cylinder (32) through the electromagnetic valve, closing the electromagnetic valve of the luciferase reagent storage tank (53) when the luciferase reagent is added to a set time point, and enabling the luciferase reagent to react with ATP to generate luminescent fluorescent substances, so that all bacteria on the surface of the biological film (19) are finally reacted to the fluorescent substances;

s5, detecting the light intensity of light emitted by fluorescent materials in the straight cylinder (32), wherein the specific operation steps are as follows:

s51, controlling a piston rod of a jacking cylinder (49) of an auxiliary detection assembly (26) to retract downwards, wherein the piston rod drives an L plate (28) to move downwards, and the L plate (28) drives a feeding cylinder (29), a movable plate (30), a straight cylinder (32) and a biological film installation assembly to move downwards synchronously;

S52, controlling a piston rod of the feeding oil cylinder (29) to extend rightwards, driving the movable plate (30) to move rightwards by the piston rod, driving the straight cylinder (32) and the biological film installation assembly to synchronously move rightwards by the movable plate (30), and enabling the straight cylinder (32) to just move to the position right below the shading cylinder (54) of the liquid adding and shading assembly (27) after the piston rod of the feeding oil cylinder (29) extends completely;

S53, controlling a piston rod of a jacking cylinder (49) to extend upwards, driving an L plate (28) to move upwards by the piston rod, driving a straight cylinder (32) to move upwards by the L plate (28), enabling a top end opening of the straight cylinder (32) to be just embedded into a shading cylinder (54) after the piston rod of the jacking cylinder (49) extends completely, shading the top end opening of the straight cylinder (32) by the shading cylinder (54) at the moment so as to prevent external natural light from entering a container body (17), enabling a photosensitive probe (24) of a photometer (23) to enter the straight cylinder (32), enabling the photosensitive probe (24) to detect the light intensity of light emitted by fluorescent substances, enabling the photosensitive probe (24) to convert the light intensity into an electric signal, enabling the electric signal to be transmitted to a controller by the photosensitive probe (24), and enabling the controller to calculate the content of bacteria in a pipe column (3) according to the electric signal;

When the detected bacterial content is higher than the specified content, the method indicates that the quantity of bacteria entering the tubular column (3) is large, at the moment, a worker regulates the discharge capacity of the variable pump (12) to increase the quantity of the sterilization corrosion inhibitor sprayed into the tubular column (3) so as to prevent the bacteria from corroding the tubular column (3) and further protect the tubular column (3), when the detected bacterial content is lower than the specified content, the method indicates that the quantity of bacteria entering the tubular column (3) is small, at the moment, the worker regulates the discharge capacity of the variable pump (12) to decrease the quantity of the sterilization corrosion inhibitor sprayed into the tubular column (3), and further the use quantity of the sterilization corrosion inhibitor is saved;

S6, after the detection is finished, the operation steps of pouring the fluorescent material and the residual reagent in the straight cylinder (32) into the waste liquid tank (56) by workers are as follows:

s61, controlling a piston rod of a jacking cylinder (49) of the auxiliary detection assembly (26) to retract downwards, so that the straight cylinder (32) and the biological film installation assembly synchronously move downwards;

S62, controlling a piston rod of a vertical oil cylinder (45) of an auxiliary detection assembly (26) to extend downwards so that a plug (47) just exits from a central hole of a lower annular clamping plate (42) of the biological film installation assembly, then controlling a hydraulic motor (44) to rotate, driving the vertical oil cylinder (45), a lifting plate (46) and the plug (47) to synchronously rotate, and controlling the hydraulic motor (44) to be closed by a controller after the plug (47) rotates 180 degrees, wherein residual reagent in a straight cylinder (32) sequentially passes through the central hole of an upper annular clamping plate (43), the biological film (19) and the central hole of the lower annular clamping plate (42) and finally falls into a waste liquid tank (56), so that the residual reagent is discharged into the waste liquid tank (56);

S63, controlling a piston rod of a main oil cylinder (35) of an auxiliary detection assembly (26) to extend downwards, driving a lifting plate (36) to move downwards by the piston rod, driving a secondary oil cylinder (37), a rack (38), a connecting plate (39), a gear (41), a rotating shaft (40) and a biological film installation assembly to synchronously move downwards by the lifting plate (36), and separating the biological film installation assembly from a straight cylinder (32) just after the piston rod of the main oil cylinder (35) extends completely;

S64, controlling a piston rod of a secondary oil cylinder (37) of an auxiliary detection assembly (26) to retract upwards, enabling the piston rod to drive a rack (38) to move upwards, enabling the rack (38) to drive a gear (41) to rotate, enabling a rotating shaft (40) to rotate through the gear (41), enabling a biological film installation assembly to rotate synchronously, enabling the biological film installation assembly to be converted into a vertical state after the piston rod of the secondary oil cylinder (37) is completely retracted, enabling fluorescent substances on the surface of a biological film (19) to fall into a waste liquid groove (56) through a central hole of an upper annular clamping plate (43), and accordingly enabling the fluorescent substances to be poured into the waste liquid groove (56) to prepare for secondary bacterial content.