CN119454495A - Composition for rapidly removing black eye and long-acting anti-aging and application thereof - Google Patents

Abstract

The invention discloses a composition and application thereof for rapidly removing dark circles and long-term anti-aging, and the components of the composition include: β-nicotinamide mononucleotide, glutathione, acetyl hexapeptide-8, acetyl tetrapeptide-5, snake venom peptide, arginine/lysine polypeptide, tocopherol acetate and ergothioneine. The present invention adopts a multi-pronged approach, and plays the effects of repairing, restoring elasticity, anti-aging, etc. while removing dark circles, so as to block the generation of dark circles, and also ensure the normal function of skin. The composition of the present invention has the effect of rapidly removing dark circles, and when the composition is contained in eye cream, the eye cream can effectively remove dark circles, and the effect is obvious within 15 minutes, and the effect is rapid; long-term use can remove dark circles and play a good control effect.

Description

Composition for rapidly removing black eye and long-acting anti-aging and application thereof

Technical Field

The application relates to the technical field of cosmetics, in particular to a composition for rapidly removing dark circles and resisting aging for a long time and application thereof.

Background

The formation of black eye is affected by various factors, long-term stay up, irregular work and rest, insufficient sleep can lead to the blood vessel blood flow velocity of the skin of the eyes to be too slow to form stagnation, insufficient oxygen supply of tissues, excessive accumulation of metabolic wastes in blood vessels to cause pigmentation of the eyes, and along with continuous accumulation of melanin, the black eye appears on the skin of the eyes. And with the age, the epidermis tissue of the eye skin is dried and thinned, collagen and elastin are gradually lost, and the eye metabolism is slow, so that the eye marks, the black eye and the eye bags are formed. The black eye is classified into a cyan black eye, which is due to the stagnation of venous blood of microvasculature and slow metabolism of metabolic waste in veins, and a brown black eye, which is mainly due to pigmentation of eyes caused by melanin generation and insufficient metabolism.

In order to remove dark circles and delay skin aging around eyes, in daily life, besides ensuring sufficient sleep and developing good living habits and eye habits, the common method is to use eye cosmetics, and most of eye cosmetics in the market at present have unobvious use effects or single effects, and some products can take effect quickly, but after a period of use, side effects are easy to generate or bring new problems such as fat particles and the like, so that the eye burden is increased. Therefore, there is a need for an eye cream product that can quickly remove dark circles and is effective against aging for a long period of time.

Disclosure of Invention

In order to solve the problems, the invention provides a composition for rapidly removing dark circles and resisting aging for a long time and application thereof. The composition prepared in the invention is composed of beta-nicotinamide mononucleotide, polypeptide compound a, polypeptide compound b, tocopheryl acetate and ergothioneine, can solve the root causes of dryness, fine lines, looseness, eye bags and black eyes of eye skin caused by the cell level, is applied to the development of eye care products, has excellent effects of moisturizing, fading fine lines, tightening and removing the eye bags and the black eyes, and can comprehensively improve the skin function and younger the skin after long-term use.

In order to achieve the above object, the present invention provides a composition for rapidly removing dark circles and for long-acting anti-aging, comprising beta-nicotinamide mononucleotide, polypeptide complex a, polypeptide complex b, tocopheryl acetate, and ergothioneine.

Optionally, the composition comprises, by mass, 0.1-2 parts of beta-nicotinamide mononucleotide, 0.052-2.15 parts of polypeptide complex a, 0.002-0.2 parts of polypeptide complex b, 0.1-2 parts of tocopheryl acetate, and 0.01-0.05 parts of ergothioneine.

Preferably, the composition comprises, in parts by mass, 0.5-1.5 parts of beta-nicotinamide mononucleotide, 0.11-1.04 parts of polypeptide complex a, 0.02-0.1 parts of polypeptide complex b, 0.5-1.5 parts of tocopheryl acetate, and 0.02-0.04 parts of ergothioneine;

preferably, the composition comprises 1 part by mass of beta-nicotinamide mononucleotide, 0.52 part by mass of polypeptide complex a, 0.065 part by mass of polypeptide complex b, 1 part by mass of tocopheryl acetate, and 0.03 part by mass of ergothioneine.

Optionally, the polypeptide compound a comprises 0.05-2 parts of glutathione, 0.001-0.1 part of acetyl hexapeptide-8 and 0.001-0.05 part of acetyl tetrapeptide-5, and the polypeptide compound b comprises 0.001-0.1 part of snake venom peptide and 0.001-0.1 part of arginine/lysine polypeptide.

Preferably, the polypeptide compound a comprises 0.1-1 part of glutathione, 0.005-0.02 part of acetyl hexapeptide-8 and 0.005-0.02 part of acetyl tetrapeptide-5, wherein the polypeptide compound b comprises 0.01-0.05 part of snake venom peptide and 0.01-0.05 part of arginine/lysine polypeptide;

Preferably, the polypeptide complex a comprises 0.5 part of glutathione, 80.01 parts of acetyl hexapeptide and 0.01 part of acetyl tetrapeptide, and preferably, the polypeptide complex b comprises 0.025 part of snake venom peptide and 0.04 part of arginine/lysine polypeptide.

The invention also provides a preparation method of the emulsion for rapidly removing dark circles and resisting aging for a long time, which comprises the following steps:

s1, stirring and mixing the grease composition, a thickener and a humectant to obtain a phase A;

s2, stirring and mixing beta-nicotinamide mononucleotide, ergothioneine, polypeptide complex a and water to obtain a phase B;

S3, stirring and mixing the tocopheryl acetate, the polypeptide compound b and the emulsifier to obtain a C phase;

s4, uniformly stirring A, B, C phases to obtain emulsion.

Optionally, in the step S1, the grease composition is at least one of polydimethylsiloxane, cyclopentadimethicone, polydimethylsiloxane cross-linked polymer, cyclopentadimethicone, dodecylbenzenesulfonic acid TEA salt, the thickener is at least one of acryloyldimethyl taurate amine/VP copolymer, carbomer, sodium acrylate/acryloyldimethyl taurate amine/isohexadecane/polysorbate-80, and the humectant is at least one of hyaluronic acid and sodium hyaluronate.

Optionally, in the step S3, the emulsifier is at least one of glycerol, phenoxyethanol and ethylhexyl glycerol.

Optionally, in the step S4, the A, B, C phase pH is adjusted to 6.2, and the emulsion is prepared after stirring and mixing for 30min under a 40 ℃ water bath.

The invention also provides the application of the composition in cosmetics, health products and medicines, wherein the application comprises that the composition can be prepared into emulsion, solution, paste, gel or spray.

The synergistic effect mechanism among the components in the application is as follows:

The beta-nicotinamide mononucleotide adopted in the application can be converted into nicotinamide adenine dinucleotide (NAD ⁺ coenzyme) in cells, so that the beta-nicotinamide mononucleotide can be supplemented to improve the NAD ⁺ coenzyme content in the body, restore mitochondrial energy, activate organism energy metabolism, stimulate metabolism and repair of various aging tissues, prolong the cell life, improve the ATP generation amount and further improve the organism metabolism capacity, thereby delaying, improving, preventing aging and the like, or improving age-induced metabolic disorders, senile diseases and the like.

The polypeptide compound a adopted in the application comprises glutathione, acetyl hexapeptide-8 and acetyl tetrapeptide-5, wherein the glutathione is a tripeptide consisting of glutamic acid, cysteine and glycine, can help to maintain the normal immune system function, has the functions of antioxidation and integrated detoxification, and the acetyl hexapeptide-8 can instantly remove dynamic lines, tighten skin, imitate the N-terminal of SNAP-25, compete with natural protein at the binding site in the SNARE protein compound, lead neuron vesicles to not effectively release neurotransmitters, lead muscle contraction to be weakened, effectively and rapidly prevent the formation of the dynamic lines, effectively reorganize collagen elasticity, increase the activity of elastin, relax facial lines, improve relaxation, and the acetyl tetrapeptide-5 has the effects of preventing liquid accumulation in eye bags, fading dark circles, increasing the permeability of blood vessels, inhibiting angiotensin converting enzyme, inhibiting collagen glycosylation, increasing skin elasticity, promoting skin smoothness and the like.

The polypeptide compound b used in the application comprises a snake venom-like peptide and an arginine/lysine polypeptide, wherein the snake venom-like peptide (INCI name is dipeptide diamino Ding Xianbian-base amide diacetate) mainly acts on a protruding posterior model, is an antagonist reversible to a muscle nicotinic acetylcholine receptor (nmAChR), is combined with an epsilon subunit of nmAChR to block the combination of acetylcholine and the receptor, finally leads to receptor blocking, can not absorb sodium ions in a blocking state, can not depolarize, blocks nerve excitation transmission and relaxes muscles, thus smoothing wrinkles and not affecting expression of facial expression, and the arginine/lysine polypeptide is a biological simulation conotoxin peptide, provides very strong muscle relaxing effect through sequencing and artificial synthesis, and provides a rapid and instant anti-wrinkle effect. Arginine/lysine polypeptides are highly folded peptides, penetrating strongly, highly stable, targeted against muscle relaxation.

The tocopherol acetate used in the application is used as vitamin E derivative, and has the effect of preventing the oxidation of cell membranes, intracellular unsaturated fatty acids and the like in the skin metabolism process, thereby protecting the integrity of the cell membranes and further preventing aging. The external product contains tocopheryl acetate and has strong reducibility, and can eliminate cell free radicals and reduce the damage of ultraviolet rays to skin.

The ergothioneine (L-Ergothioneine, EGT) adopted in the application is a rare natural chiral amino acid, is an important natural active substance in a body, plays roles in resisting oxidation, scavenging singlet oxygen, hydroxyl free radicals, hypochlorous acid and hydrogen peroxide free radicals, detoxifies, maintaining the biosynthesis of DNA, the normal growth of cells and cellular immunity, preventing ultraviolet radiation damage, regulating oxidation-reduction reaction in cells, participating in the regulation of intracellular energy, the physiological protection of cells and the like.

The beneficial effects of the invention are as follows:

1. The invention reasonably compounds beta-nicotinamide mononucleotide, glutathione, acetyl hexapeptide-8, acetyl tetrapeptide-5, snake venom peptide, arginine/lysine polypeptide, tocopheryl acetate and ergothioneine, utilizes the synergistic effect of different 'nerve inhibitors' of the arginine/lysine polypeptide, the acetyl hexapeptide-8 and the snake venom peptide to relax eye muscles aiming at dynamic lines and static lines around eyes so as to reduce the formation of fine lines, changes the microcirculation of the eye area through acetyl tetrapeptide-5, changes the osmotic pressure of liquid so as to reduce edema, thereby effectively eliminating edema, eye bags and black eyes, and simultaneously forms a renewable antioxidant network system by taking glutathione, tocopheryl acetate and ergothioneine and beta-nicotinamide mononucleotide, and has synergistic effects of inhibiting tyrosinase activity and scavenging free radicals, thus better resisting oxidation and saccharification. The product can be used for a long time, and can achieve the effects of long-term aging resistance by enabling the skin to be revived and youthful from instant to long-term and from the outside to the inside.

2. The invention has the functions of repairing, recovering elasticity, resisting aging and the like while eliminating black eyes under multiple tubes, thereby preventing the generation of black eyes and ensuring the normal function of skin; in addition, the composition provided by the invention has the effect of rapidly removing the black eye, when the composition is contained in the eye cream, the eye cream can effectively remove the black eye, has obvious effect within 15 minutes, takes effect rapidly, and can remove the black eye and has good control effect after long-term use.

3. The raw materials provided by the invention are environment-friendly and healthy, have no toxic or side effect and no dependency, can be used for a long time, have the effects of activating cells, promoting collagen synthesis, scavenging free radicals, inhibiting tyrosinase activity and the like, and have the effects of whitening skin, removing freckles and reducing wrinkles, and the skin state is improved in all directions, so that the long-acting anti-aging effect is achieved, and the raw materials can be used as emulsion, solution, paste, gel or spray type singly or matched with other skin care products.

Detailed Description

In order to more clearly illustrate the general inventive concept, a detailed description is given below by way of example.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than as described, and therefore the scope of the present invention is not limited to the specific embodiments disclosed below.

The starting materials, reagents or apparatus used in the examples which follow are commercially available from conventional sources or may be obtained by methods known in the art unless otherwise specified, and the methods used in the examples of the present invention are all well known to those skilled in the art. Wherein the arginine/lysine polypeptide is L-arginine/L-lysine polypeptide with CAS number 31014-78-5, which is purchased from Shanghai daydream medical science and technology Co.

Hereinafter, the composition having the rapid black eye removal and long-acting anti-aging functions of the present invention, and the preparation method and application thereof will be described in detail with reference to exemplary embodiments.

The specific embodiment of the application is to better embody the efficacy of the composition, and the composition is prepared into emulsion form for experimental test, but the application is not limited to one product form of emulsion in specific application, for example, the composition can be prepared into solution, paste, gel or spray form and other product forms.

Example 1 preparation of the composition

The preparation method of the product 1# comprises the following steps:

1. 3g of polydimethylsiloxane, 3g of cyclopentamethylsiloxane, 18g of polydimethylsiloxane cross-linked polymer (and) cyclopentamethylsiloxane (and) dodecyl benzene sulfonic acid TEA salt, 0.1g of carbomer, 0.55g of acrylamide/VP copolymer, 0.8g of sodium acrylate/acrylamide/cetyl isoester/polysorbate-80 and 1g of sodium hyaluronate are stirred and dispersed uniformly to obtain phase A;

2. 1g of beta-nicotinamide mononucleotide, 0.5g of glutathione, 0.01g of acetyl hexapeptide-8, 0.01g of acetyl tetrapeptide-5, 0.03g of ergothioneine and 66g of water are stirred and dispersed uniformly to obtain a phase B;

3. Mixing tocopheryl acetate 1g, snake venom peptide 0.025g, arginine/lysine polypeptide 0.04g, glycerol 4g, phenoxyethanol 0.9g, and ethylhexyl glycerol 0.1g, stirring and dispersing to obtain phase C;

4. Mixing A, B, C phases, adding citric acid to adjust the pH value to 6.2,40 ℃ and carrying out water bath for 30min, and stirring and mixing to obtain emulsion No. 1.

The product with the functions of rapidly removing dark circles and resisting aging for a long time is named as product No. 1.

According to the preparation method of product 1#, component amounts are adjusted to prepare product 1# -12# and product D1# -D4#, the specific formula is shown in table 1, product 1# -12# is an experimental group, and product D1# -D4# is a control group.

Table 1 formulation of the components of the product

Example 2

An emulsion product was prepared by the method of reference example 1, except that example 2 was set according to the parameter conditions in table 1, and the other conditions were the same as in example 1, to prepare product # 2.

Example 3

An emulsion product was prepared by the method of reference example 1, except that example 3 was set according to the parameter conditions in table 1, and the other conditions were the same as in example 1, to prepare product # 3.

Example 4

An emulsion product was prepared by the method of reference example 1, except that example 2 was set according to the parameter conditions in table 1, and the other conditions were the same as in example 1, to prepare product # 2.

Example 5

An emulsion product was prepared by the method of reference example 1, except that example 2 was set according to the parameter conditions in table 1, and the other conditions were the same as in example 1, to prepare product # 2.

Example 6

An emulsion product was prepared by the method of reference example 1 and example 6 was set up according to the parameters in Table 1, except that carnosine, acetyl tetrapeptide-2 was used instead of glutathione, acetyl hexapeptide-8, and the other conditions were the same as in example 1, to prepare product No. 6.

Example 7

An emulsion product was prepared by the method of reference example 1 and example 7 was set up according to the parameters conditions in Table 1, except that oligopeptide-1, oligopeptide-3 was used in place of acetyl hexapeptide-8, acetyl tetrapeptide-5, and the other conditions were the same as in example 1, to prepare product # 7.

Example 8

An emulsion product was prepared by the method of reference example 1 and example 8 was set up according to the parameters set forth in Table 1, except that palmitoyl pentapeptide-4 was used in place of the snake venom peptide, and the other conditions were the same as in example 1, to prepare product # 8.

Example 9

An emulsion product was prepared by the method of reference example 1 and example 9 was set up according to the parameters set forth in Table 1, except that pentapeptide-3 was used in place of the arginine/lysine polypeptide, and the other conditions were the same as in example 1, to prepare product # 9.

Example 10

An emulsion product was prepared by the method of reference example 1 and example 10 was set up according to the parameter conditions in table 1, except that the raw materials of the components in phase a, phase B, and phase C were directly subjected to simple physical mixing, and the other conditions were the same as in example 1, to prepare product # 10.

Example 11

An emulsion product was prepared by the method of reference example 1, and example 11 was set up according to the parameter conditions in Table 1, except that the beta-nicotinamide mononucleotide, ergothioneine, and tocopheryl acetate components were adjusted in parts, and the other conditions were the same as in example 1, to prepare a product 11#.

Example 12

An emulsion product was prepared by the method of reference example 1, and example 12 was set up according to the parameter conditions in table 1, except that the component parts of polypeptide complex a and polypeptide complex b were adjusted, and the other conditions were the same as in example 1, to prepare a product 12#.

Comparative example D1#)

An emulsion product was prepared by the method of reference example 1, and comparative example D1# was set according to the parameter conditions in Table 1, except that tripeptide-1 copper was used instead of beta-nicotinamide mononucleotide, and the other conditions were the same as in example 1, to prepare the product D1#.

Comparative example D2#)

An emulsion product was prepared by the method of reference example 1 and comparative example d2# was set according to the parameter conditions in table 1, except that carnosine, tocopherol phosphate was used instead of ergothioneine, tocopherol acetate, and the other conditions were the same as in example 1, to prepare the product d2#.

Comparative example D3#)

An emulsion product was prepared by the method of reference example 1, and comparative example D3# was set according to the parameter conditions in Table 1, except that carnosine, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 were used instead of glutathione, acetyl hexapeptide-8, acetyl tetrapeptide-5, and the other conditions were the same as in example 1, to prepare the product D3#.

Comparative example D4#)

An emulsion product was prepared by the method of reference example 1 and comparative example D4# was set according to the parameters set forth in Table 1, except that Pterocarpin-4, acetyl dipeptide-1 was used in place of the snake venom peptide, arginine/lysine polypeptide, and the other conditions were the same as in example 1 to prepare product D4#.

Experimental example 1

The safety, the black eye removing effect and the anti-aging effect of the eye cream are tested, and the testing method and the results are as follows:

1. Human body skin spot pasting experiment

The test method comprises the steps of testing products 1-12# and D1-D4# by adopting a closed patch experiment in a skin patch experiment of human body in chapter 2015 of cosmetic health Specification, selecting 64 healthy skin volunteers aged 18-40 years, dividing each half of men and women into 16 groups randomly, wherein 4 persons (2 men and 2 women) in each group are selected, 1-12 groups are used as products 1-12# and 13-16 groups are used as products D1-D4#. The reaction criteria are shown in Table 2 and the test results are shown in Table 3.

TABLE 2 criteria for human skin reactions

TABLE 3 test results of human skin Patch test

The test results show that the product prepared by the application has no irritation and good safety to skin by carrying out the closed patch test on the products prepared by the application for each group of subjects, and observing skin reactions for 0.5, 24 and 48 hours, wherein 0 cases show skin adverse reactions.

2. Test of effect of removing black eyes

Test method 80 female volunteers (age 40-50 years, average age 45 years) were selected and randomized on average into 16 groups. After each group of subjects cleans the face with warm water, the test before use is carried out after resting for 0.5-1h in a constant temperature and humidity room with the temperature of 25 ℃ and the humidity of 40-60%, the corresponding products are respectively coated on the lower eyelid and the outer canthus of the testers for 15 minutes, then the testers are uncovered and massaged for absorption, wherein 1-12 groups use products 1-12#,13-16 groups use products D1-D4#, and the test and self-evaluation are carried out after use. Test skin was tested for skin texture, brightness (averaged) using a PIXIENCE company C-Cube multi-function skin imaging analysis system and the tester self-assessed for periocular skin gloss and black eye level. The test results are shown in Table 4.

TABLE 4 test statistics of the effect of removing dark circles

Note that L values represent brightness, where complete darkness (l=0) to absolute whiteness (l=100);

ITA DEG (individual type angle) represents skin tone less than 10 DEG brown, 10-28 DEG brown, 28-41 DEG normal, 41-55 DEG off white, and more than 55 DEG very white.

As can be seen from Table 4, the L value and ITA degree of the eye skin of the test subjects using the products 1 to 12# are obviously improved, and the improvement effect of the test subjects using the products 1 to 5# is more obvious, especially the improvement effect of the product 2# is optimal, which indicates that the skin at the black eye is brighter and whiter, the eye cream has the effects of rapidly fading the black eye and brightening the eye skin color, and when the active substances are 1 part of beta-nicotinamide mononucleotide, 0.5 part of glutathione, 0.01 part of acetyl hexapeptide-8,0.01 part of acetyl tetrapeptide-5, 0.025 part of snake venom peptide, 0.04 part of arginine/lysine polypeptide, 1 part of tocopheryl acetate and 0.03 part of ergothioneine, the improvement effect is optimal, and the synergistic effect of removing the black eye of the active substances is further proved.

3. Anti-aging effect verification test

The test method comprises selecting 80 female volunteers (age 40-50 years, average age 45 years), dividing into 16 groups at random, cleaning face every day in the morning and evening, respectively corresponding to the products prepared in the application examples, wherein 1-12 groups respectively correspond to 1-12# and 13-16 groups respectively correspond to 1-D4# of the application products, each group has the same using amount, detecting with VISIA instrument after 8 weeks of continuous use, and evaluating the wrinkle area, wrinkle number and wrinkle depth reduction degree of the fish tail line area of the eyes, and the test results are shown in Table 5.

TABLE 5 statistical test of anti-aging effect

Experiments prove that in the long-acting anti-aging test, the products 1-12# can effectively play roles in long-acting anti-aging and reducing eye wrinkles, the effects of the products 1-5# are more obvious, especially the product 1# has the best lifting effects on long-acting anti-aging and reducing eye wrinkles, and in the use of the products D1-D4#, the long-acting anti-aging effects are far lower than the product 1#, which indicates that the active components have synergistic effects in anti-aging and better comprehensive effects, and when the active substances are 1 part of beta-nicotinamide mononucleotide, 0.5 part of glutathione, 0.01 part of acetyl hexapeptide-8,0.01 part of acetyl tetrapeptide-5, 0.025 part of snake venom peptide, 0.04 part of arginine/lysine polypeptide, 1 part of tocopheryl acetate and 0.03 part of ergothioneine, the improvement effects are the best.

The above description is only an example of the present application, and the scope of the present application is not limited to the specific examples, but is defined by the claims of the present application. Various modifications and variations of the present application will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the technical idea and principle of the present application should be included in the protection scope of the present application.

Claims (10)

1. A composition for rapidly removing dark circles and resisting aging for a long time, which is characterized by comprising beta-nicotinamide mononucleotide, a polypeptide compound a, a polypeptide compound b, tocopheryl acetate and ergothioneine.

2. The composition according to claim 1, wherein the composition comprises, in parts by mass, 0.1-2 parts of beta-nicotinamide mononucleotide, 0.052-2.15 parts of polypeptide complex a, 0.002-0.2 parts of polypeptide complex b, 0.1-2 parts of tocopheryl acetate, and 0.01-0.05 parts of ergothioneine.

3. The composition according to claim 1, wherein the composition comprises, in parts by mass, 0.5-1.5 parts of β -nicotinamide mononucleotide, 0.11-1.04 parts of polypeptide complex a, 0.02-0.1 parts of polypeptide complex b, 0.5-1.5 parts of tocopheryl acetate, 0.02-0.04 parts of ergothioneine;

preferably, the composition comprises 1 part by mass of beta-nicotinamide mononucleotide, 0.52 part by mass of polypeptide complex a, 0.065 part by mass of polypeptide complex b, 1 part by mass of tocopheryl acetate, and 0.03 part by mass of ergothioneine.

4. The composition according to claim 1, wherein the polypeptide complex a comprises 0.05-2 parts by mass of glutathione, 0.001-0.1 part by mass of acetyl hexapeptide-8, 0.001-0.05 part by mass of acetyl tetrapeptide-5, and the polypeptide complex b comprises 0.001-0.1 part by mass of snake venom-like peptide and 0.001-0.1 part by mass of arginine/lysine polypeptide.

5. The composition according to claim 1, wherein the polypeptide complex a comprises 0.1-1 part of glutathione, 0.005-0.02 part of acetyl hexapeptide-8, 0.005-0.02 part of acetyl tetrapeptide-5, 0.01-0.05 part of snake venom-like peptide and 0.01-0.05 part of arginine/lysine polypeptide;

Preferably, the polypeptide complex a comprises 0.5 part of glutathione, 80.01 parts of acetyl hexapeptide and 0.01 part of acetyl tetrapeptide, and preferably, the polypeptide complex b comprises 0.025 part of snake venom peptide and 0.04 part of arginine/lysine polypeptide.

6. The preparation method of the emulsion for rapidly removing dark circles and resisting aging for a long time is characterized by comprising the following steps:

s1, stirring and mixing the grease composition, a thickener and a humectant to obtain a phase A;

s2, stirring and mixing beta-nicotinamide mononucleotide, ergothioneine, polypeptide complex a and water to obtain a phase B;

S3, stirring and mixing the tocopheryl acetate, the polypeptide compound b and the emulsifier to obtain a C phase;

s4, uniformly stirring A, B, C phases to obtain emulsion.

7. The method according to claim 6, wherein in the step S1, the grease composition is at least one of polydimethylsiloxane, cyclopentadimethicone, dimethicone cross polymer, cyclopentadimethicone, and dodecyl benzene sulfonic acid TEA salt, the thickener is at least one of acrylamide/VP copolymer, carbomer, sodium acrylate/acrylamide/isohexadecane/polysorbate-80, and the humectant is at least one of hyaluronic acid and sodium hyaluronate.

8. The method according to claim 6, wherein in the step S3, the emulsifier is at least one of glycerol, phenoxyethanol, and ethylhexyl glycerol.

9. The method according to claim 6, wherein in the step S4, the A, B, C phase is adjusted to pH 6.2 and mixed in a water bath at 40 ℃ for 30min to obtain the emulsion.

10. Use of a composition according to any one of claims 1-5 in cosmetics, health products, pharmaceuticals, said use comprising the composition being formulated as an emulsion, solution, paste, gel or spray.