Detailed Description
The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by a person skilled in the art without any inventive effort, are intended to be within the scope of the present invention, based on the embodiments of the present invention.
Example 1
A preparation method of a plant matrix with long water retention time and good mechanical property comprises the following steps,
S1, adding polyvinyl alcohol and agarose into deionized water, and stirring and mixing uniformly at 95 ℃ to obtain a mixed solution, wherein the concentration of the polyvinyl alcohol in the mixed solution is 1.5%, and the concentration of the agarose in the mixed solution is 1%;
s2, filling the mixed solution and the matrix raw materials into a mould container with a cylinder of which the length, width and height are 10cm, 10cm and 6cm respectively and the bottom is provided with a cylinder of which the diameter is 4cm and the height is 2.5cm, wherein the matrix raw materials consist of peat, perlite and vermiculite according to the weight ratio of 8:4:1, and the volume of the mixed solution is 0.7;
S3, carrying out three freeze-thawing cycles on the mixed solution and the matrix raw material in the mold, wherein each cycle is frozen at-20 ℃ for 16 hours, and thawing at normal temperature for 8 hours to obtain the plant matrix 1.
Example 2
A preparation method of a plant matrix with long water retention time and good mechanical property comprises the following steps,
S1, adding polyvinyl alcohol and agarose into deionized water, and uniformly stirring and mixing at 95 ℃ to obtain a mixed solution, wherein the concentration of the polyvinyl alcohol in the mixed solution is 1.5%, and the concentration of the agarose in the mixed solution is 0.5%;
S2, filling the mixed solution and the matrix raw materials into a mould container with a cylinder of which the length, width and height are 10 cm-6 cm respectively and the bottom is provided with a cylinder of which the diameter is 4cm and the height is 2.5cm, wherein the matrix raw materials consist of peat, perlite and vermiculite according to the weight ratio of 8:4:1, and the volume of the mixed solution is 0.9
S3, carrying out three freeze-thawing cycles on the mixed solution and the matrix raw material in the mold, wherein each cycle is frozen at-20 ℃ for 16 hours, and thawing at normal temperature for 8 hours to obtain the plant matrix 2.
Example 3
A preparation method of a plant matrix with long water retention time and good mechanical property comprises the following steps,
S1, adding polyvinyl alcohol and agarose into deionized water, and uniformly stirring and mixing at 95 ℃ to obtain a mixed solution, wherein the concentration of the polyvinyl alcohol in the mixed solution is 1%, and the concentration of the agarose in the mixed solution is 1%;
S2, filling the mixed solution and the matrix raw materials into a mould container with a cylinder of which the length, width and height are 10 cm-6 cm respectively and the bottom is provided with a cylinder of which the diameter is 4cm and the height is 2.5cm, wherein the matrix raw materials consist of peat, perlite and vermiculite according to the weight ratio of 8:4:1, and the volume of the mixed solution is 1.1
S3, carrying out three freeze-thawing cycles on the mixed solution and the matrix raw material in the mold, wherein each cycle is frozen at-20 ℃ for 16 hours, and thawing at normal temperature for 8 hours to obtain the plant matrix 3.
Example 4
A preparation method of a plant matrix with long water retention time and good mechanical property comprises the following steps,
S1, adding polyvinyl alcohol and agarose into deionized water, and stirring and mixing uniformly at 95 ℃ to obtain a mixed solution, wherein the concentration of the polyvinyl alcohol in the mixed solution is 1%, and the concentration of the agarose in the mixed solution is 0.5%;
S2, filling the mixed solution and the matrix raw materials into a mould container with a cylinder of which the length, width and height are 10cm, 10cm and 6cm respectively and the bottom is provided with a cylinder of which the diameter is 4cm and the height is 2.5cm, wherein the matrix raw materials consist of peat, perlite and vermiculite according to the weight ratio of 8:4:1, and the volume of the mixed solution is 1.2;
s3, carrying out three freeze-thawing cycles on the mixed solution and the matrix raw material in the mold, wherein each cycle is frozen at-20 ℃ for 16 hours, and thawing at normal temperature for 8 hours to obtain the plant matrix 4.
Example 5
A preparation method of a plant matrix with long water retention time and good mechanical property comprises the following steps,
S1, adding polyvinyl alcohol and agarose into deionized water, and stirring and mixing uniformly at 95 ℃ to obtain a mixed solution, wherein the concentration of the polyvinyl alcohol in the mixed solution is 3%, and the concentration of the agarose in the mixed solution is 0.25%;
s2, filling the mixed solution and the matrix raw materials into a mould container with a cylinder of which the length, width and height are 10 cm-6 cm respectively and the bottom is provided with a cylinder of which the diameter is 4cm and the height is 2.5cm, wherein the matrix raw materials consist of peat, perlite and vermiculite according to the weight ratio of 8:4:1, and the volume of the mixed solution is 0.7
S3, carrying out three freeze-thawing cycles on the mixed solution and the matrix raw material in the mold, wherein each cycle is frozen at-20 ℃ for 16 hours, and thawing at normal temperature for 8 hours to obtain the plant matrix 5.
Example 6
A preparation method of a plant matrix with long water retention time and good mechanical property comprises the following steps,
S1, adding polyvinyl alcohol and agarose into deionized water, and uniformly stirring and mixing at 95 ℃ to obtain a mixed solution, wherein the concentration of the polyvinyl alcohol in the mixed solution is 3%, and the concentration of the agarose in the mixed solution is 2%;
S2, filling the mixed solution and the matrix raw materials into a mould container with a cylinder of which the length, width and height are 10 cm-6 cm respectively and the bottom is provided with a cylinder of which the diameter is 4cm and the height is 2.5cm, wherein the matrix raw materials consist of peat, perlite and vermiculite according to the weight ratio of 8:4:1, and the volume of the mixed solution is 0.9
S3, carrying out three freeze-thawing cycles on the mixed solution and the matrix raw material in the die, wherein each cycle is frozen at-20 ℃ for 16 hours, and thawing at normal temperature for 8 hours to obtain the plant matrix 6.
Example 7
A preparation method of a plant matrix with long water retention time and good mechanical property comprises the following steps,
S1, adding polyvinyl alcohol and agarose into deionized water, and uniformly stirring and mixing at 95 to obtain a mixed solution, wherein the concentration of the polyvinyl alcohol in the mixed solution is 1%, and the concentration of the agarose in the mixed solution is 0.25%;
S2, filling the mixed solution and the matrix raw materials into a mould container with a cylinder of which the length, width and height are 10 cm-6 cm respectively and the bottom is provided with a cylinder of which the diameter is 4cm and the height is 2.5cm, wherein the matrix raw materials consist of peat, perlite and vermiculite according to the weight ratio of 8:4:1, and the volume of the mixed solution is 1.1
S3, carrying out three freeze-thawing cycles on the mixed solution and the matrix raw material in the mold, wherein each cycle is frozen at-20 ℃ for 16 hours, and thawing at normal temperature for 8 hours to obtain the plant matrix 7.
Example 8
A preparation method of a plant matrix with long water retention time and good mechanical property comprises the following steps,
S1, adding polyvinyl alcohol and agarose into deionized water, and stirring and mixing uniformly at 95 ℃ to obtain a mixed solution, wherein the concentration of the polyvinyl alcohol in the mixed solution is 0.5%, and the concentration of the agarose in the mixed solution is 2%;
s2, filling the mixed solution and the matrix raw materials into a mould container with a cylinder of which the length, width and height are 10 cm-6 cm respectively and the bottom is provided with a cylinder of which the diameter is 4cm and the height is 2.5cm, wherein the matrix raw materials consist of peat, perlite and vermiculite according to the weight ratio of 8:4:1, and the volume of the mixed solution is 1.2
S3, carrying out three freeze-thawing cycles on the mixed solution and the matrix raw material in the die, wherein each cycle is frozen at-20 ℃ for 16 hours, and thawing at normal temperature for 8 hours to obtain the plant matrix 8.
Example 9
A preparation method of a plant matrix with long water retention time and good mechanical property comprises the following steps,
S1, adding polyvinyl alcohol and agarose into deionized water, and uniformly stirring and mixing at 95 ℃ to obtain a mixed solution, wherein the concentration of the polyvinyl alcohol in the mixed solution is 1%, and the concentration of the agarose in the mixed solution is 1%;
S2, filling the mixed solution and the matrix raw materials into a mould container with a cylinder of which the length, width and height are 10 cm-6 cm respectively and the bottom is provided with a cylinder of which the diameter is 4cm and the height is 2.5cm, wherein the matrix raw materials consist of peat, perlite and vermiculite according to the weight ratio of 8:4:1, and the volume of the mixed solution is 1.1
S3, performing freeze-thawing cycle on the mixed solution and the matrix raw material in the mold twice, wherein the mixed solution and the matrix raw material are frozen at-20 ℃ for 16 hours and thawed at normal temperature of 25 ℃ for 8 hours in each cycle, so as to obtain the plant matrix 9.
Example 10
A preparation method of a plant matrix with long water retention time and good mechanical property comprises the following steps,
S1, adding polyvinyl alcohol and agarose into deionized water, and uniformly stirring and mixing at 95 ℃ to obtain a mixed solution, wherein the concentration of the polyvinyl alcohol in the mixed solution is 1.5%, and the concentration of the agarose in the mixed solution is 0.5%;
s2, filling the mixed solution and the matrix raw materials into a mould container with a cylinder of which the length, width and height are 10cm, 10cm and 6cm respectively and the bottom is provided with a cylinder of which the diameter is 4cm and the height is 2.5cm, wherein the matrix raw materials consist of peat, perlite and vermiculite according to the weight ratio of 8:4:1, and the volume of the mixed solution is 0.9;
and S3, performing four freeze-thawing cycles on the mixed solution and the matrix raw material in the mold, wherein the mixed solution and the matrix raw material are frozen at-20 ℃ for 16 hours and thawed at normal temperature of 25 ℃ for 8 hours in each cycle, so as to obtain the plant matrix 10.
Test results
1. Determination of mechanical Properties of plant substrates
The impact resistance of the plant matrix was tested using a pendulum impact tester, and the surface hardness of the matrix material in the dry and wet state was tested using a shore durometer, respectively, with the results shown in table 1.
TABLE 1
2. Determination of pore structure of plant substrates
The pore structure of the matrix is a space capable of containing water and air among solid particles of the matrix material, and is also a space for the activities of animal microorganisms among plant root systems and the matrix, and the reasonable pore structure is very important for the growth condition of plants.
The porosity of a matrix refers to the percentage of pore volume per volume of the total volume of the matrix. The matrix porosity was defined as matrix porosity (%) = (1-matrix raw material volume/matrix volume) ×100% and the results are shown in table 2.
TABLE 2
3. Determination of the Water-holding Property of plant substrates
The water content of the plant matrix is the gram of water content held in each hundred grams of matrix, the water retention is calculated according to the following formula, wherein the water retention is measured= (W Wet state -W Dry )/W Dry is 100 percent), 10cm is 6cm square fiber culture soil is soaked for 10 minutes to fully absorb water, the plant matrix is spread to dryness and weighed, then the plant matrix is baked to constant weight in an oven at 65 ℃, and the plant matrix is moved into a dryer to be cooled to room temperature, and is weighed, and the result is shown in a table 3.
TABLE 3 Table 3
4. Observation of plant substrate stability
After harvesting the lettuce, observing whether the matrix block collapses, the volume is shrunken, the appearance changes such as cracking and the like. Collapse, volume shrinkage to less than 95% before cultivation, cracking, and cracking were recorded as 1 score, cracking was recorded as 2 scores for two of the three cases, cracking was recorded as 3 scores for three of the three cases, and cracking was recorded as 0 score if the three cases were not present, and substrate blocks were not cured and molded, and the results are shown in table 4.
TABLE 4 Table 4
5. Plant growth observation
Lettuce was sown in each run and observed for growth for 4 weeks (28 days total). The planted lettuce has strong plants, thick leaves, normal leaf color, strong root system, well developed capillary root and poor growth condition. In addition, after the lettuce grows for four weeks, the fresh weight and the dry weight of the single lettuce plant are recorded, and the more the fresh weight and the dry weight of the lettuce plant with good growth vigor are, the more excellent the lettuce cultivation effect of the substrate is considered.
TABLE 5
From the comparison of tables 1-5 above, it was found that the polyvinyl alcohol and agarose contents had a significant effect on the mechanical properties, pore structure, water holding capacity, stability and plant growth conditions of the matrix materials. And the number of freeze-thaw cycles can also affect the effect of the final matrix material.
Of examples 1-4, example 1 was 1.780 kj/square meter, the highest in terms of impact resistance. This indicates that the matrix has the strongest resistance when impacted by external forces and is suitable for use in environments where high strength protection is required. Example 2 was 1.395 kj/square meter, next highest. The impact resistance was higher but slightly lower than in example 1. Example 3, 1.195 kj/square meter, medium. The shock resistance is moderate, and the anti-shock agent is suitable for being used in general environments. Whereas example 4 was 1.002 kj/square meter, the lowest. The method is also suitable for being used in a general environment. The wet and dry matrix surface hardness tendencies are consistent with impact resistance, with example 1 being highest, example 2 times highest, example 3 medium, example 4 lowest. In combination with plant growth, example 1 is too hard, affecting plant root growth and expansion, and also limiting root respiration and moisture uptake. While examples 2-4, which are more suitable in hardness, are beneficial to the growth of plant root systems and the respiration and moisture absorption of the root systems. Also, the plants grew significantly worse in example 1 than in examples 2-4.
In examples 1-4, matrix porosity plays a critical role in the plant growth process, as it directly affects the air permeability and water retention of the matrix, thereby affecting respiration and moisture uptake of the plant root system. The lower porosity of example 1 means that the air permeability of the matrix is poor, which may limit the oxygen supply to the root system and affect the respiration of the root system. And example 1 also has relatively low water holding capacity (376%) combined with low porosity, the mobility of the moisture in the matrix may be poor, resulting in difficulty in obtaining sufficient moisture for the root system.
The lettuce growth was therefore poor, with both fresh and dry weights being minimal, indicating that low porosity and water retention are detrimental to lettuce growth.
In comparison, the porosity and water retention of the examples 2-4 are higher, the air permeability is better, the oxygen supply of the root system is facilitated, and the respiration of the root system is promoted. Sufficient moisture can also be provided, which is beneficial to the plants to keep the moisture under drought conditions.
By comparing the porosity of the matrix of the four examples and their effect on lettuce growth, it can be seen that the matrix of example 4 has the highest porosity, and the best water holding capacity and air permeability, which is most beneficial to lettuce growth. Example 1 had the lowest porosity, poor water retention and air permeability, resulting in the worst lettuce growth. Both the porosity and the water holding capacity of example 2 and example 3 are moderate, which is favorable for lettuce growth, but slightly inferior to example 4.
By comparing the matrix stability, impact resistance and surface hardness of examples 1-4, it can be seen that example 4 performed the worst in matrix stability because of its lower impact resistance and surface hardness, resulting in collapse of the matrix blocks after cultivation. However, these properties do not negatively affect lettuce growth, but rather provide a more suitable growth environment due to higher water holding capacity and porosity, but may undergo structural changes during use due to poor matrix stability, affecting long term use. In comparison, examples 2 and 3 are excellent in substrate stability, no significant change and damage of the external structure occurs in the plant growth cycle, and lettuce grows well as well, and examples 2 and 3 are probably superior to example 4 if used for plant cultivation in the growth cycle.
By comparing the four examples 1-4 laterally, example 4 performs best on multiple key performance indicators if the plant is cultivated non-vertically for a short period of time, and can provide an optimal growing environment for lettuce. But is only applicable to short-term and general-scene cultivation because of poor substrate stability due to its low impact resistance and surface hardness.
However, it can be found from examples 5, 6 and 8 that the soilless culture substrate blocks for plant cultivation can be produced without simply curing them, and that too high concentration of polyvinyl alcohol and agarose can result in too hard substrate blocks and difficulty in normal growth of plants. The mechanical properties of the whole of example 5, example 6 and example 8 are very excellent, and the solidification of the matrix material can be completely realized, but the whole matrix is too hard to be used for plant cultivation. In addition, in the cultivation process of example 6 and example 8, the situation that the plants cannot be rooted into the matrix blocks after seedling transplanting due to the too high surface hardness occurs, and the lettuce cannot be planted, but in the cultivation process of example 5, although the lettuce can grow by rooting, the root system is difficult to grow, so that insufficient water, nutrient and air are obtained, and the lettuce growth condition is extremely poor. In contrast to example 7, the immobilization of the matrix block was not achieved after three freeze-thaw cycles, since the polyvinyl alcohol and agarose in the mixture were too low. Therefore, the proper proportion of polyvinyl alcohol and agarose can obviously influence the mechanical property, pore structure, water holding capacity and stability of the matrix block, thereby influencing the growth of plants.
Comparing example 3 with example 9, example 2 and example 10 can find that the better the matrix material effect is as the number of freeze-thaw cycles increases, the less well the matrix mass can be set after two freeze-thaw cycles as in example 9, and the curing effect cannot be achieved. And example 3 with the same proportion has excellent mechanical property after three freeze thawing cycles, and the lettuce cultivation effect is also good. However, by comparing example 2 with example 10, no significant difference in properties was found, which can be explained by the fact that the hydrogels were able to achieve excellent crosslinking after three freeze-thawing cycles and thus had less change after the fourth freeze-thawing cycle. And (3) taking the energy consumption cost of the time cost grade freeze thawing cycle into consideration, wherein the freeze thawing cycle is completed for 3 times.
In the comprehensive view, the embodiment 2 and the embodiment 3 are balanced in various performance indexes, are favorable for growth of lettuce, have excellent stability and can be suitable for cultivation in multiple scenes and long periods.
The foregoing is merely a preferred embodiment of the invention, and it is to be understood that the invention is not limited to the form disclosed herein but is not to be construed as excluding other embodiments, but is capable of numerous other combinations, modifications and environments and is capable of modifications within the scope of the inventive concept, either as taught or as a matter of routine skill or knowledge in the relevant art. And that modifications and variations which do not depart from the spirit and scope of the invention are intended to be within the scope of the appended claims.