CN119408831A - A cell transport box for maintaining biological cell activity - Google Patents
A cell transport box for maintaining biological cell activity Download PDFInfo
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- CN119408831A CN119408831A CN202510035711.0A CN202510035711A CN119408831A CN 119408831 A CN119408831 A CN 119408831A CN 202510035711 A CN202510035711 A CN 202510035711A CN 119408831 A CN119408831 A CN 119408831A
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- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 18
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Abstract
本发明涉及生物工程技术领域，且公开了一种保持生物细胞活性的细胞运输箱，包括细胞转运箱，细胞转运箱内部设置有试管架，细胞转运箱的外表面固定连通有水箱，细胞转运箱的外表面固定安装有水泵，细胞转运箱内部设置有用于使试管架能被方便快捷地取出的快取组件，通过密封门板在开启的过程中，能够带动试管架整体滑出装置内部，进而此结构实现了多重改进和优势，首先此结构提高了操作的便捷性，操作人员无需费力地将试管架逐一取出，操作人员只需滑动密封门板，试管架即可整体滑出，从而极大地简化了操作步骤，其次，由于试管架在滑出的过程中保持了稳定，减少了倾斜、晃动和掉落的风险，从而避免了细胞样本因撞击而受损。
The present invention relates to the field of bioengineering technology, and discloses a cell transport box for maintaining the activity of biological cells, comprising a cell transfer box, a test tube rack being arranged inside the cell transfer box, an outer surface of the cell transfer box being fixedly connected to a water tank, a water pump being fixedly installed on the outer surface of the cell transfer box, a quick-access component for enabling the test tube rack to be taken out conveniently and quickly being arranged inside the cell transfer box, and the test tube rack can be driven to slide out of the device as a whole during the process of opening a sealed door panel, thereby achieving multiple improvements and advantages of this structure. Firstly, this structure improves the convenience of operation, and an operator does not need to laboriously take out the test tube racks one by one, and the operator only needs to slide the sealed door panel, and the test tube rack can slide out as a whole, thereby greatly simplifying the operation steps. Secondly, since the test tube rack remains stable during the sliding out process, the risks of tilting, shaking and falling are reduced, thereby avoiding damage to the cell sample due to impact.
Description
Technical Field
The invention relates to the technical field of bioengineering, in particular to a cell transport case for maintaining activity of biological cells.
Background
The cell transport case is a device specially designed for transporting and preserving living cells or tissues, and its main purpose is to ensure that cells or tissues maintain their activity during transportation so as to be able to perform further study or treatment after reaching a destination, and has been widely used in fields of biomedical research, biotechnology, medical diagnosis, and treatment, etc.
However, with the continuous development of technology, the existing cell transportation box also gradually exposes some problems, the existing cell transportation box usually adopts a mode of forming ventilation holes on a box body or adopting mechanical ventilation to realize ventilation effect, but the existing ventilation technology has a non-negligible problem that dust invades, the cell transportation box is transported by different transportation means, including land transportation means, sea transportation means, aircrafts and the like, wherein no matter which transportation means is transported, dust inevitably enters the cell transportation box through the ventilation holes, and the dust is easy to carry microorganisms such as bacteria and viruses and can directly contact with cells to cause physical damage.
When the dust enters the cell transportation box, the clean environment in the box is damaged at first, the cell has extremely high requirement on the cleanliness of the environment, any tiny pollutants can cause abnormal reaction of the cell, microorganisms such as bacteria and viruses in the dust can invade the cell once contacting the cell to damage the normal physiological function of the cell, even lead to cell death, further the cell transportation box cannot guarantee the activity of the cell transportation box when transporting biological cells, further the preservation effect of the cell transportation box on the biological cells is reduced, in addition, the dust can carry chemical substances such as heavy metals, harmful gases and the like, and the substances have toxic effects on the cells.
Meanwhile, when the cell transport case is used, because the cell transport case is designed initially to accommodate and transport a large amount of cell samples, the samples are usually placed in the test tube rack to be orderly and stable, however, when the test tube rack needs to be placed in an overlapping manner to save space, operators often need to integrally take out the whole test tube rack from the inside of the cell transport case in order to take out or put in the test tube rack, and the process is not only tedious, but also very easy to damage the test tube rack and the cell samples therein under the condition of limited space.
In order to take out the test tube rack, the operator usually needs to open the cover of the cell transport case, because the inner space of the transport case is limited, the opening angle of the cover is limited, so that the operator needs to take out the test tube rack with great care to avoid collision with the case, even a skilled operator works in a limited space, the collision and friction are difficult to avoid completely, and secondly, when the test tube rack is taken out integrally, the operator has difficulty in stably grasping all the test tube racks at one time due to a certain gap between the test tube racks placed in an overlapping manner, which increases the risks of tilting, shaking or even dropping the test tube rack in the process of taking out or placing back, and once the test tube rack is tilted or shaken, the cell sample therein is damaged due to collision, thereby affecting the activity of cells.
For this purpose, a cell transport container is proposed which retains the activity of biological cells.
Disclosure of Invention
The present invention is directed to a cell transport case for maintaining biological cell activity, which solves the above-mentioned problems of the prior art.
In order to achieve the above object, the invention provides a cell transport box for maintaining biological cell activity, which comprises a cell transport box, wherein a test tube rack is arranged inside the cell transport box, the outer surface of the cell transport box is fixedly communicated with a water tank, the outer surface of the cell transport box is fixedly provided with a water pump, a cache component for enabling the test tube rack to be conveniently and rapidly taken out is arranged inside the cell transport box, a buffer component for preventing the cell transport box from shaking in the transport process is arranged below the cell transport box, a ventilation and dust prevention component for providing air for cells and isolating dust is arranged outside the cell transport box, and a temperature control component for automatically adjusting the temperature inside the cell transport box is arranged outside the cell transport box.
The quick-access assembly comprises an L-shaped groove and a sealing door plate, wherein the L-shaped groove is symmetrically formed in the inner surface of the cell transfer box, the top and the bottom of the sealing door plate are slidably connected in the L-shaped groove, the inner surface of the cell transfer box is symmetrically formed in a linear groove, the test tube rack is slidably connected in the linear groove, a limit column is symmetrically and fixedly connected to the bottom of the side edge of the test tube rack, a rotation limit rod is symmetrically and rotatably connected to the inner wall of the cell transfer box, the limit column is slidably connected to the inner part of the rotation limit rod, and a reset spring is fixedly connected between the bottom surface of the test tube rack and the inner wall of the cell transfer box.
The dustproof subassembly of ventilation includes the ventilation casing, ventilation casing symmetry fixed connection is in the surface of cell transfer case, all eccentric rotation is connected with the dwang on the inner wall of ventilation casing, circular slot has been seted up to the symmetry on the inner wall of ventilation casing, the surface of dwang all is annular equidistance and arranges rotates and be connected with the blade, the air inlet has all been seted up to the internal surface of ventilation casing, the gas outlet has all been seted up on the inner wall of ventilation casing runs through the surface of cell transfer case.
Preferably, the buffer assembly comprises two vertical spring groups, wherein the two vertical spring groups are fixedly connected to the bottom of the cell transfer box respectively, the bottom of the vertical spring groups is fixedly connected with a supporting bottom plate, the two ends of the vertical spring groups, which are close to each other, are fixedly connected with a bearing plate, the outer surface of the bearing plate is symmetrically and rotationally connected with a rotating support, the upper surface of the supporting bottom plate is fixedly connected with a fixing rod, the surface of the fixing rod is symmetrically and slidingly connected with a sliding sleeve, the top of the sliding sleeve is rotationally connected with the bottom of the rotating support, the two ends of the sliding sleeve, which are close to each other, are fixedly connected with transverse springs, and the transverse springs are slidingly connected to the surface of the fixing rod.
Preferably, the temperature control assembly comprises a temperature control pipeline, the temperature control pipeline is fixedly connected to the outer surface of one side of the cell transfer box, which is far away from the sealing door plate, two ends of the temperature control pipeline are respectively communicated with the water tank and the water pump, a connecting plate is fixedly connected to the inner wall of the temperature control pipeline, a temperature bulb is fixedly connected to the outer surface of the connecting plate, and a valve core is fixedly connected to the surface of one side of the temperature bulb, which is far away from the connecting plate.
Preferably, the rotating block is arranged on the external motor in a driving way, and the external motor is started and closed under the electrical control of the external controller, so that the rotating block is driven to rotate by the rotation of the output shaft of the external motor.
Preferably, the cell transfer box is internally provided with a water pipeline, the water tank and the water pump are communicated with the water pipeline, the shape of the valve core is matched with the shape of the inner wall of the temperature control pipeline, the shape is matched, and the cell transfer box can be tightly matched.
Preferably, a refrigerating element is arranged in the water tank, and the refrigerating element and the water pump are electrically controlled to be started and closed by an external controller.
Preferably, a locating pin is clamped between the sealing door plate and the outer surface of the cell transfer box.
Preferably, the ethylene glycol is arranged in the temperature bulb, so that the effect that the ethylene glycol can change the temperature in time under the characteristic of higher temperature sensitivity of the ethylene glycol is achieved.
Compared with the prior art, the invention has the beneficial effects that:
1. Through the centrifugal force that the blade that the decentration set up produced to and the design of air inlet departure centre of a circle, blockked effectively the entering of impurity such as dust, through utilizing the blade that the decentration set up, produce outside centrifugal force when the blade rotates, and then centrifugal force can get rid of the air inlet through impurity such as the dust of air inlet entering incasement, thereby prevent impurity such as dust entering cell transport incasement portion, simultaneously when the air current passes through eccentric air inlet, the air current can receive the influence of rotatory effect, has further strengthened the inside effect of prevention dust entering ventilation casing.
Firstly, the structure obviously improves the sealing performance and the protective performance of the cell transport case, effectively prevents the entry of pollutants such as dust, bacteria, viruses and the like, and can keep a cleaner and stable environment in the transport process of cells, thereby reducing the risk of infection and physical damage of the cells.
Secondly, this structure improves the preservation environment of the cells, which, thanks to the effective isolation of dust and contaminants, can maintain their activity in a safer and more stable environment, which is critical for maintaining the physiological function of the cells and the success of subsequent experiments.
In addition, through avoiding the entering of impurity such as dust, also reduced death and the damage that the cell leads to because of the pollution, this has important meaning to improving the survival rate of cell transportation and guaranteeing the quality of cell sample, still helps reducing experimental failure and the repeated operation that lead to because of cell damage to improve the efficiency and the accuracy of experiment.
2. Through sealed door plant at the in-process of opening, can drive the whole roll-off device of test-tube rack inside, and then realized multiple improvement, at first, the convenience of operation has been improved, operating personnel need not hard take out the test-tube rack one by one, operating personnel only need slide sealed door plant, the test-tube rack can wholly roll-off, thereby operating procedure has been simplified, secondly, because the test-tube rack has kept stable at the in-process of roll-off, the risk of slope, rocking and dropping has been reduced, thereby the cell sample is impaired because the striking has been avoided, simultaneously because no longer need the operating personnel manual whole take out the test-tube rack from inside the cell transfer case, and then the collision and the friction of test-tube rack and cell transfer case have been reduced, also reduced the risk that the cell sample received the pollution, error and repeated work in the operation process have been reduced, because the test-tube rack can wholly roll-off device inside, operating personnel can observe the state of cell sample in the test-tube rack more directly perceivedly, the possibility of maloperation has been reduced, and because operating process is simple and fast more, operating personnel's work burden and tired also reduce, and the work efficiency of operating personnel has been improved.
3. Through the ingenious cooperation of horizontal spring and vertical spring group, the cell transfer case has shown horizontal shock attenuation and vertical shock attenuation dual effect on overall structure, not only strengthened the stability and the shock resistance of cell transfer case, still for the cell provides safer, stable environment in the transportation, at first absorbed the impact force on the horizontal direction, reduced the displacement and the rocking of cell transfer case in the horizontal direction, thereby the stability of cell transfer case has been improved, vertical spring group then is responsible for absorbing the impact force on the vertical direction simultaneously, the vibration of cell transfer case in the vertical direction has been buffered effectively, further strengthened the shock resistance of cell transfer case, and because the stability and the shock resistance of cell transfer case have obtained the promotion, the physical damage and the impact that the cell received reduce, thereby the death rate of cell has been reduced.
4. The moving distance of the valve core is controlled through the characteristic of thermal expansion and cold contraction of the temperature bulb, the effect of cell preservation is obviously improved, long-time heat exchange between water and the refrigerating element is ensured, so that a stable and proper temperature environment is maintained, the survival time of cells is prolonged, the preservation effect of the cells is improved, a higher-quality cell sample is provided for subsequent cell culture and experiments, the closing of the valve core is controlled through the characteristic of thermal expansion and cold contraction of the temperature bulb, the temperature can be controlled more accurately, the influence of temperature fluctuation on the cells is reduced, the stability in the transportation process is improved, the death rate of the cells is reduced, and a more reliable cell sample is provided for biomedical research and medical diagnosis.
Drawings
FIG. 1 is a schematic perspective view of a main structure of the present invention;
FIG. 2 is a rear perspective view of the main structure of the present invention;
FIG. 3 is a schematic cross-sectional perspective view of the internal connections of the inventive cache module;
FIG. 4 is an enlarged view of the invention at A in FIG. 3;
FIG. 5 is an enlarged view of the invention at B in FIG. 3;
FIG. 6 is a schematic perspective view showing the connection of the linear groove and the cell transfer box;
FIG. 7 is a schematic cross-sectional perspective view of a cache assembly of the present invention;
FIG. 8 is an enlarged view of FIG. 7 at C in accordance with the present invention;
FIG. 9 is a schematic cross-sectional perspective view of the vent dust assembly of the present invention;
FIG. 10 is an enlarged view of the invention at D in FIG. 9;
FIG. 11 is a schematic cross-sectional perspective view of a temperature control assembly of the present invention;
fig. 12 is an enlarged view of fig. 11 at E in accordance with the present invention.
In the figure:
1. the cell transfer box, 2, a test tube rack, 3, a water tank, 4, a water pump;
the quick-access component comprises a 51, an L-shaped groove, a 52, a sealing door plate, a 53, a linear groove, a 54, a limit post, a 55, a rotation limit rod, a 56 and a return spring;
the buffer assembly comprises 61, a vertical spring group, 62, a supporting bottom plate, 63, a bearing plate, 64, a rotating bracket, 65, a fixed rod, 66, a sliding sleeve and 67, and a transverse spring;
The ventilation dustproof component comprises a ventilation shell 71, a rotation block 72, a circular groove 73, a blade 74, a blade 75, an air inlet 76 and an air outlet;
The temperature control assembly comprises 81 parts of temperature control pipelines, 82 parts of connecting plates, 83 parts of temperature packs, 84 parts of valve cores.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to fall within the scope of the invention.
Referring to fig. 1 to 12, an embodiment of the present invention is provided:
The utility model provides a keep living cell transport case of biological cell, including cell transport case 1, cell transport case 1 embeds the water pipe, water tank 3 and water pump 4 all communicate with the water pipe, the shape of case 84 and the shape looks adaptation of control by temperature change pipe 81 inner wall have been played, can tight fit's effect, cell transport case 1 inside is provided with test-tube rack 2, cell transport case 1's fixed surface intercommunication has water tank 3, the built-in refrigeration component of water tank 3, refrigeration component starts and closes by external controller electrical control with water pump 4, cell transport case 1's surface fixed mounting has water pump 4, cell transport case 1 inside is provided with the quick-access subassembly that is used for making test-tube rack 2 can be taken out by convenient and fast, cell transport case 1's below is provided with the buffer assembly that is used for preventing cell transport case 1 production rocking in the transportation, cell transport case 1's outside is provided with the ventilation subassembly that is used for providing air and isolated dust for the cell transport case 1, cell transport case 1's outside is provided with the control by temperature subassembly that is used for automatically regulated cell transport case 1 inside temperature.
The quick-access assembly comprises an L-shaped groove 51 and a sealing door plate 52, wherein the L-shaped groove 51 is symmetrically formed in the inner surface of the cell transfer box 1, the top and the bottom of the sealing door plate 52 are both in sliding connection with the inside of the L-shaped groove 51, a locating pin is clamped between the sealing door plate 52 and the outer surface of the cell transfer box 1, a linear groove 53 is symmetrically formed in the inner surface of the cell transfer box 1, a test tube rack 2 is in sliding connection with the inside of the linear groove 53, a limit post 54 is symmetrically and rotationally connected to the side bottom of the test tube rack 2, a rotation limit rod 55 is symmetrically and rotationally connected to the inner wall of the cell transfer box 1, the limit post 54 is all in sliding connection with the inside of the rotation limit rod 55, a reset spring 56 is fixedly connected between the bottom surface of the test tube rack 2 and the inner wall of the cell transfer box 1, the stiffness coefficient of the reset spring 56 is insufficient to damage cells in the test tube rack 2, and the reset spring 56 can still return the test tube rack 2 to the cell transfer box 1 by means of self elasticity.
The ventilation dustproof assembly comprises a ventilation shell 71, the ventilation shell 71 is symmetrically and fixedly connected to the outer surface of the cell transfer box 1, rotating blocks 72 are eccentrically and rotationally connected to the inner wall of the ventilation shell 71, the rotating blocks 72 are drivingly installed on an external motor, the external motor is electrically controlled to be started and closed by an external controller, the effect that an output shaft of the external motor rotates to drive the rotating blocks 72 to rotate is achieved, circular grooves 73 are symmetrically formed in the inner wall of the ventilation shell 71, blades 74 are rotationally connected to the surface of the rotating blocks 72 in annular equidistant arrangement, air inlets 75 are formed in the inner surface of the ventilation shell 71, and air outlets 76 are formed in the inner wall of the ventilation shell 71 penetrating through the surface of the cell transfer box 1.
The buffer assembly comprises two vertical spring groups 61, the two vertical spring groups 61 are respectively fixedly connected to the bottom of the cell transfer box 1, a supporting bottom plate 62 is fixedly connected to the bottom of the two vertical spring groups 61, a bearing plate 63 is fixedly connected to one end, close to the two vertical spring groups 61, of the bearing plate 63, a rotating support 64 is symmetrically and rotatably connected to the outer surface of the bearing plate 63, a fixing rod 65 is fixedly connected to the upper surface of the supporting bottom plate 62, sliding sleeves 66 are symmetrically and slidably connected to the surface of the fixing rod 65, the tops of the sliding sleeves 66 are both rotatably connected to the bottom of the rotating support 64, transverse springs 67 are fixedly connected to one end, close to the two sliding sleeves 66, of the two sliding sleeves 67, and the transverse springs 67 are slidably connected to the surface of the fixing rod 65.
The control by temperature change subassembly includes control by temperature change pipeline 81, control by temperature change pipeline 81 fixed connection keeps away from the one side surface of sealed door plant 52 in cell transfer case 1, control by temperature change pipeline 81's both ends are linked together with water tank 3 and water pump 4 respectively, fixedly connected with connecting plate 82 on the control by temperature change pipeline 81 inner wall, the surface fixedly connected with temperature bulb 83 of connecting plate 82, the inside ethylene glycol that is provided with of temperature bulb 83 has played the effect that the ethylene glycol can make timely change to the temperature under the higher characteristic of self temperature sensitivity, one side surface fixedly connected with case 84 of connecting plate 82 is kept away from to temperature bulb 83.
The working principle of the invention is as follows:
the initial state is that the top of the sealing door plate 52 is located at one side of the vertical edge in the L-shaped groove 51, the test tube rack 2 is located at one side of the linear groove 53 far away from the sealing door plate 52, the limiting column 54 is located at one side of the rotation limiting rod 55 far away from the top of the cell transfer box 1, the reset spring 56 is not stretched, and the vertical spring group 61 and the transverse spring 67 are not compressed.
The following steps are specifically executed:
The cells are put into the test tube rack 2, as shown in fig. 1 to 6, the operator manually removes the positioning pins on the surface of the sealing door plate 52 and presses the bottom surface of the sealing door plate 52, and at this time, the sealing door plate 52 is wholly pressed by the operator, so that the movement trend towards the water pump 4 is generated, but the sealing door plate 52 is limited by the L-shaped groove 51, so that the bottom of the sealing door plate 52 slides towards the water pump 4 towards the side of the horizontal edge of the L-shaped groove 51 in compliance with the pressing force applied by the operator, and at the same time, the top of the sealing door plate 52 also slides towards the bottom of the cell transfer box 1 at the side of the vertical edge of the L-shaped groove 51.
As shown in fig. 7, when the bottom of the sealing door plate 52 enters the cell transfer box 1, the bottom of the sealing door plate 52 will collide with the bottom of the rotation limiting rod 55, and the rotation limiting rod 55 rotates with the center as a rotation pivot, so that the rotation limiting rod 55 makes the test tube rack 2 generate the same rotation trend through the limiting post 54, but the test tube rack 2 and the limiting post 54 cannot rotate under the limitation of the linear slot 53, so that under the guiding effect of the linear slot 53, the test tube rack 2 converts the rotation trend into the sliding motion towards the water pump 4, further at this time, the test tube rack 2 slides in the linear slot 53 towards the side away from the water pump 4, after the limiting post 54 slides to the top of the rotation limiting rod 55, the whole test tube rack 2 slides out of the cell transfer box 1, in the process, the reset spring 56 is pulled by the test tube rack 2 and is in a stretching state, and at this moment, the test tube rack 2 is integrally exposed outside, at this moment, the operator clamps the sealing door plate 52 on the surface of the cell transfer box 1 again through the locating pin, and put the test tube containing cells into the test tube rack 2, in the process of opening through the sealing door plate 52, can drive the test tube rack 2 to integrally slide out of the device, and further realize multiple improvements, firstly, the convenience of operation is improved, the operator does not need to take out the test tube rack 2 one by one, the operator only needs to slide the sealing door plate 52, the test tube rack 2 can integrally slide out, and therefore, the operation steps are simplified, secondly, because the test tube rack 2 keeps stable in the sliding-out process, the risks of tilting, shaking and dropping are reduced, so that the damage to cell samples due to collision is avoided, meanwhile, the operator is not required to manually take out the test tube rack 2 integrally from the cell transfer box 1, furthermore, the collision and friction between the test tube rack 2 and the cell transfer box 1 are reduced, the risk that the cell sample is polluted is also reduced, errors and repeated labor in the operation process are reduced, an operator can more intuitively observe the state of the cell sample in the test tube rack 2 because the test tube rack 2 can integrally slide out of the device, the possibility of misoperation is reduced, and the workload and fatigue feeling of the operator are also reduced because the operation process is simpler, more convenient and faster, and the working efficiency of the operator is further improved.
As shown in fig. 1 to 7, after the test tube is assembled, the operator removes the positioning pin, at this time, the sealing door plate 52, the test tube rack 2, the limiting post 54 and the rotation limiting rod 55 are not subjected to any external force except for the gravity of the operator, and then the return spring 56 pulls the test tube rack 2 back to the initial state under the action of the elastic contraction of the return spring 56, as the above steps indicate, when the return spring 56 is stretched, the sealing door plate 52 is located inside the cell transfer box 1, and then the return spring 56 contracts at this time, the sealing door plate 52 is driven to return to the initial state again by the limiting post 54 and the rotation limiting rod 55, at this time, the operator clamps the sealing door plate 52 on the surface of the cell transfer box 1 again by the positioning pin, at this time, the cells are already loaded into the cell transfer box 1, and the cell transfer box 1 can be transported.
During transportation of automobiles or trains, the cell transfer box 1 is often subjected to vibrations of different directions and intensities due to the complexity of road conditions and other external factors.
As shown in fig. 3 to 5, when the vehicle passes over a bumpy road surface or a train passes over a rail joint, when the cell transfer box 1 is subjected to vibration from a vertical direction, the vertical spring set 61 starts to act, at this time, the vertical spring set 61 is in a continuous compression and extension process due to external vibration, and in this process, the vertical spring set 61 converts part of kinetic energy into elastic potential energy, so that direct influence of vibration on the cell transfer box 1 is reduced, meanwhile, the vertical spring set 61 drives the receiving plate 63 to synchronously move towards the supporting bottom plate 62 when contracting, and the lowering of the receiving plate 63 causes a certain rotation of the rotating bracket 64, under the limitation of the fixed rod 65, the rotating bracket 64 also pushes the sliding sleeve 66 to slide towards one side, which is close to the two sliding sleeves 66, further, in the process that the two sliding sleeves 66 slide on the fixed rod 65, the transverse spring 67 is pushed, in the compression state, at this time, the vertical spring set 61 is in the process of being continuously compressed and extended due to vibration, and in the process that the transverse spring 67 is also in the continuous compression and extension state, the process of the transverse spring 67 is in the process of directly influencing the cell transfer box 1 due to the external vibration potential energy.
As shown in fig. 3 to 5, when the vehicle turns or brakes emergently, the cell transfer box 1 receives pushing force or pulling force from the side, the transverse spring 67 is responsible for processing vibration from the horizontal direction, the transverse spring 67 synchronously transmits vibration force to the vertical spring group 61 through the sliding sleeve 66, the rotating bracket 64 and the bearing plate 63, and then the vertical spring group 61 and the transverse spring 67 synchronously disperse the impact force through own elasticity at this time, so as to reduce the vibration of the cell transfer box 1 in the horizontal direction, and the vertical and horizontal vibration can cause the vertical and horizontal vibration of the vertical spring group 61 and the transverse spring 67 to absorb the vibration reasonably, so that the cell transfer box 1 is better protected, the vibration of the cell transfer box 1 is reduced, the ingenious matching of the transverse spring 67 and the vertical spring group 61 is realized, the cell transfer box 1 shows dual effects of horizontal shock absorption and vertical shock absorption on the whole structure, stability and shock resistance of the cell transfer box 1 are enhanced, safer and stable environments are provided for cells in the transportation process, impact force on the horizontal direction is firstly absorbed, displacement and shaking of the cell transfer box 1 in the horizontal direction are reduced, stability of the cell transfer box 1 is improved, meanwhile, the vertical spring group 61 is responsible for absorbing the impact force on the vertical direction, vibration of the cell transfer box 1 in the vertical direction is effectively buffered, shock resistance of the cell transfer box 1 is further enhanced, and physical damage and impact to the cells are reduced due to the stability and shock resistance of the cell transfer box 1, so that death rate of the cells is reduced.
As shown in fig. 9 and 10, during transportation, an operator needs to electrically control the external motor to start through the external controller, the output shaft of the external motor rotates to drive the rotating block 72 to synchronously rotate, and the rotating block 72 drives the vane 74 on the surface of the rotating block to synchronously rotate along the track of the circular groove 73.
In terms of ventilation, since both the air inlet 75 and the air outlet 76 are offset from the center of the ventilation housing 71, when air passes through the air outlets 76, the air does not flow straight along the center of the ventilation housing 71, but moves along an inclined path, and thus the air generates a rotational component when entering the ventilation housing 71, which helps to guide the air more smoothly through the ventilation housing 71.
Meanwhile, when the circular groove 73 rotates, the vane 74 fixed to the rotating block 72 rotates with it, and the rotating vane 74 forms a dynamic rotating passage which varies with the rotation of the vane 74, but always maintains a certain space for air to pass through, and at the same time, the inclined flow path helps to reduce the resistance of air flow, so that air can pass through the ventilation housing 71 more smoothly.
The structure utilizes Bernoulli principle in fluid mechanics, in incompressible fluid, the pressure is small at the place with high flow rate, the pressure is strong at the place with low flow rate, because the air passes through the ventilation shell 71, the flow path is inclined, and the air continuously changes along with the rotation of the vane 74, the flow rate of the air passes through the ventilation shell 71 is changed, the pressure is changed due to the change of the flow rate, so that a dynamic pressure field is formed, the pressure field helps to guide the air to pass through the ventilation shell 71 more smoothly, the ventilation effect is enhanced, and further air enters the ventilation shell 71 through the air inlet 75 and finally enters the cell transfer box 1 through the air outlet 76 for cell respiration.
In terms of dust protection, the rotation of the vane 74 within the circular groove 73 plays a vital role, the vane 74 not only forms a dynamic barrier, but also effectively prevents dust from entering the interior of the ventilation housing 71 by the centrifugal force generated by its rotation, and as the vane 74 rotates within the circular groove 73, the vane 74 constantly changes its position relative to the rotating block 72, which rotation forms a dynamic barrier, such that dust encounters the constantly changing vane 74 position when attempting to enter the ventilation housing 71, thereby increasing the difficulty of dust entry.
Second, since the mass of dust is greater than that of air, the dust is more easily affected by centrifugal force when the vane 74 rotates, and the centrifugal force is an outward force applied to the object when it rotates, which causes foreign substances such as dust to be pushed to a side away from the air inlet 75, and the effect of the centrifugal force further enhances the dust prevention effect of the ventilation dust prevention assembly.
In addition, the eccentric design of the ventilation dustproof assembly plays a key role, because the rotating block 72 deviates from the circle center of the ventilation shell 71, when the rotating block 72 drives the blades 74 to rotate, the blades 74 move along an inclined path, and the inclined moving path helps to enhance the ventilation effect, meanwhile, when air flows, dust is more easily influenced by gravity to be settled downwards, the inclined path enables the dust to be subjected to the combined action of gravity and centrifugal force when passing through the air inlet 75, the gravity pulls the dust to the bottom of the air inlet 75, the centrifugal force pushes the dust to the periphery of the air inlet 75, the dust is more difficult to enter the ventilation shell 71 under the combined action of gravity and centrifugal force, so that the dustproof effect of the ventilation dustproof assembly is enhanced under the combined action of the gravity and the centrifugal force, the inlet 75 is deviated from the circle center through the centrifugal force generated by the eccentrically arranged blades 74, the inlet 75 is effectively blocked from entering of impurities such as dust, the dust is more easily influenced by the gravity generated when the eccentrically arranged blades 74 rotate, the dust and the like are further centrifugal force can be tried to enter the air inlet 75 to be thrown away from the bottom of the air inlet 75, and the dust and the like in the air inlet 75 are prevented from entering the inside of the air inlet 71 through the air inlet 1 due to the combined action of the centrifugal force, and the centrifugal force is prevented from entering the inside of the air inlet 71.
Firstly, the structure obviously improves the sealing performance and the protective performance of the cell transfer box 1, effectively prevents the entry of pollutants such as dust, bacteria, viruses and the like, and can keep a cleaner and stable environment in the transportation process of cells, thereby reducing the risk of infection and physical damage of the cells.
Secondly, this structure improves the preservation environment of the cells, which, thanks to the effective isolation of dust and contaminants, can maintain their activity in a safer and more stable environment, which is critical for maintaining the physiological function of the cells and the success of subsequent experiments.
In addition, through avoiding the entering of impurity such as dust, also reduced death and the damage that the cell leads to because of the pollution, this has important meaning to improving the survival rate of cell transportation and guaranteeing the quality of cell sample, still helps reducing experimental failure and the repeated operation that lead to because of cell damage to improve the efficiency and the accuracy of experiment.
As shown in fig. 1 and 2, during the cell transportation process, an operator needs to add water into the water tank 3, and then the operator manually controls the external controller, and the external controller electrically controls the refrigeration element and the water pump 4 to be started.
When the outside temperature becomes high, the water in the water pipe is affected, and then the temperature of the water becomes high.
As shown in fig. 11 and 12, after the water pump 4 pumps the water in the water injection pipeline into the temperature control pipeline 81, the water with a higher temperature passes through the temperature-pack of the connecting plate 82 and contacts with the temperature-pack 83, in this process, the temperature-pack 83 is heated and expanded by a certain extent due to the higher temperature of the water, one end of the expanded temperature-pack 83 is fixedly connected with the connecting plate 82, and then most of the temperature-pack 83 continuously expands towards one side far away from the connecting plate 82, and the expansion of the temperature-pack 83 drives the valve core 84 to synchronously move towards the water tank 3, at this time, due to the movement of the valve core 84, the gap between the valve core 84 and the inner wall of the temperature control pipeline 81 gradually decreases, so that the water flow rate flowing through the valve core 84 decreases, and further, the flow rate of the water in the temperature control pipeline 81 at this time decreases synchronously.
When the flow rate of water is reduced, the contact time between water molecules and the refrigerating element arranged in the water tank 3 is prolonged, so that the heat exchange efficiency is improved, the heat in the water is absorbed and transferred to the refrigerating element, the contact time between the water and the refrigerating element is prolonged, the water is continuously refrigerated by the refrigerating element after heat exchange, and continuously enters the water channel arranged in the cell transfer box 1 through the water tank 3, the water is circulated, the water temperature in the water channel is kept at a proper temperature all the time to ensure the activity of cells, the moving distance of the valve core 84 is controlled through the thermal expansion and contraction characteristics of the temperature bulb 83, the effect of cell preservation is remarkably improved, the long-time heat exchange between the water and the refrigerating element is ensured, a stable and proper temperature environment is maintained, the survival time of cells is prolonged, the preservation effect of cells is improved, a cell sample with higher quality is provided for subsequent cell culture and experiments, the closing of the valve core 84 is controlled through the thermal expansion characteristics of the temperature bulb 83, the influence of the temperature fluctuation on the cells is reduced, the cell death rate is not improved, the medical diagnosis and the cell death rate is further improved, and the medical diagnosis of the cell sample is further provided.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises an element.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The cell transport box capable of keeping biological cell activity comprises a cell transport box (1), wherein a test tube rack (2) is arranged inside the cell transport box (1), a water tank (3) is fixedly communicated with the outer surface of the cell transport box (1), and a water pump (4) is fixedly arranged on the outer surface of the cell transport box (1), and the cell transport box is characterized in that a quick-taking component used for enabling the test tube rack (2) to be conveniently and rapidly taken out is arranged inside the cell transport box (1), a buffer component used for preventing the cell transport box (1) from shaking in the transport process is arranged below the cell transport box (1), a ventilation dustproof component used for providing air for cells and isolating dust is arranged outside the cell transport box (1), and a temperature control component used for automatically adjusting the temperature inside the cell transport box (1) is arranged outside the cell transport box (1);
The quick-access assembly comprises an L-shaped groove (51) and a sealing door plate (52), wherein the L-shaped groove (51) is symmetrically arranged on the inner surface of the cell transfer box (1), the top and the bottom of the sealing door plate (52) are both in sliding connection with the inside of the L-shaped groove (51), the inner surface of the cell transfer box (1) is symmetrically arranged with a linear groove (53), the test tube rack (2) is in sliding connection with the inside of the linear groove (53), the side bottom of the test tube rack (2) is symmetrically and fixedly connected with a limit column (54), the inner wall of the cell transfer box (1) is symmetrically and rotationally connected with a rotation limit rod (55), the limit column (54) is both in sliding connection with the inside of the rotation limit rod (55), and a reset spring (56) is fixedly connected between the bottom surface of the test tube rack (2) and the inner wall of the cell transfer box (1).
The utility model provides a cell transfer box, including ventilation dustproof subassembly, ventilation casing (71) symmetry fixed connection is in the surface of cell transfer box (1), all eccentric rotation is connected with rotor (72) on the inner wall of ventilation casing (71), circular slot (73) have been seted up to the symmetry on the inner wall of ventilation casing (71), rotor (72) surface all is annular equidistance and arranges rotates and be connected with blade (74), air inlet (75) have all been seted up to the internal surface of ventilation casing (71), gas outlet (76) have all been seted up on the surface that the inner wall of ventilation casing (71) runs through cell transfer box (1).
2. The cell transport case for maintaining activity of biological cells according to claim 1, wherein the buffer assembly comprises two vertical spring groups (61), the two vertical spring groups (61) are respectively and fixedly connected to the bottom of the cell transport case (1), a supporting bottom plate (62) is fixedly connected to the bottom of the two vertical spring groups (61), a bearing plate (63) is fixedly connected to one end, close to the two vertical spring groups (61), of the two vertical spring groups, a rotating bracket (64) is symmetrically and rotatably connected to the outer surface of the bearing plate (63), a fixing rod (65) is fixedly connected to the upper surface of the supporting bottom plate (62), sliding sleeves (66) are symmetrically and slidably connected to the surface of the fixing rod (65), the tops of the sliding sleeves (66) are both rotatably connected to the bottom of the rotating bracket (64), transverse springs (67) are fixedly connected to one end, close to the two sliding sleeves (66), and the transverse springs (67) are slidably connected to the surface of the fixing rod (65).
3. The cell transport case for keeping bioactivity of biological cells according to claim 1, wherein the temperature control assembly comprises a temperature control pipeline (81), the temperature control pipeline (81) is fixedly connected to the outer surface of one side of the cell transport case (1) far away from the sealing door plate (52), two ends of the temperature control pipeline (81) are respectively communicated with the water tank (3) and the water pump (4), a connecting plate (82) is fixedly connected to the inner wall of the temperature control pipeline (81), a temperature pack (83) is fixedly connected to the outer surface of the connecting plate (82), and a valve core (84) is fixedly connected to the outer surface of one side of the temperature pack (83) far away from the connecting plate (82).
4. A cell transport case for maintaining biological cell activity according to claim 1 wherein said rotating block (72) is drivingly mounted to an external motor which is electrically controlled on and off by an external controller.
5. A cell transport case for maintaining biological cell activity according to claim 3, wherein the cell transport case (1) is provided with a water pipe inside, the water tank (3) and the water pump (4) are both communicated with the water pipe, and the shape of the valve core (84) is adapted to the shape of the inner wall of the temperature control pipe (81).
6. The cell transport case for maintaining biological cell activity according to claim 1, wherein the water tank (3) is internally provided with a refrigerating element, and the refrigerating element and the water pump (4) are electrically controlled to be started and closed by an external controller.
7. The cell transport case for maintaining activity of biological cells according to claim 1, wherein a positioning pin is clamped between the sealing door plate (52) and the outer surface of the cell transport case (1).
8. A cell transport case for maintaining biological cell activity according to claim 3, wherein said temperature pack (83) is internally provided with ethylene glycol.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202510035711.0A CN119408831A (en) | 2025-01-09 | 2025-01-09 | A cell transport box for maintaining biological cell activity |
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| CN202510035711.0A CN119408831A (en) | 2025-01-09 | 2025-01-09 | A cell transport box for maintaining biological cell activity |
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| CN202510035711.0A Pending CN119408831A (en) | 2025-01-09 | 2025-01-09 | A cell transport box for maintaining biological cell activity |
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