CN119291210A

CN119291210A - Saliva detection pen and method of using the same

Info

Publication number: CN119291210A
Application number: CN202411399273.8A
Authority: CN
Inventors: 金忠其; 关洪炎
Original assignee: Zhejiang Wantaifu Biotechnology Co ltd
Current assignee: Zhejiang Wantaifu Biotechnology Co ltd
Priority date: 2024-10-09
Filing date: 2024-10-09
Publication date: 2025-01-10

Abstract

The present invention provides a saliva detection pen and a method for using the same. The detection pen includes a circuit device, a liquid suction rod, a test paper strip and a control chip; the circuit device includes a circuit line and an indicator light, and the liquid suction rod includes a liquid suction end and a delivery end; the circuit line connects the delivery end of the liquid suction rod, the indicator light and the control chip. When the sample to be tested flows and migrates from the liquid suction end of the liquid suction rod to the delivery end, the circuit is connected, and the indicator light flashes or stays on for prompting; the control chip is used to control the reaction time. The saliva detection pen provided by the present invention combines a saliva detection test paper strip with a liquid suction rod, a circuit device and a control chip, which solves the problem of errors in the results caused by factors such as inaccurate reading time and dim light.

Description

Saliva detection pen and using method thereof

Technical Field

The invention belongs to the technical field of rapid detection, and particularly relates to a saliva detection pen and a using method thereof.

Background

Saliva is a complex mixture containing not only various proteins but also DNA, RNA, fatty acids, various microorganisms, and the like. It has been found that various protein components in blood are also present in saliva, which reflects the changes in various protein levels in blood, and analysis of saliva components allows insight into body health and disease states. A variety of proteomic, transcriptomic and microbial markers in saliva have been identified as effective indicators for diagnosing oral and systemic diseases.

Saliva has been used for the detection of HIV, HBV viruses, and various drugs such as cocaine, alcohol. The research of saliva detection just starts, and saliva detection products adopted in the prior art need strict reading time, but can cause result difference because the products have no time prompt when in use and read outside the time required by the instruction, and meanwhile, the products need naked eye reading, and sometimes can cause inaccurate reading because of environmental difference, such as dim light, when the result is read.

Patent document CN108051579a discloses a portable aids saliva detector, which is provided with a circuit board and a shell, wherein the circuit board comprises a plurality of structures such as an indicator lamp, a buzzer, a temperature sensor and the like, when the portable aids saliva detector is used, the outer shell cap is required to be unscrewed, saliva is put into the outer shell cap with diluent to be stirred and mixed uniformly, the inner shell cap is unscrewed to screw the outer shell cap with the liquid and the shell of the portable aids saliva detector, and at the moment, the aids saliva detection test paper is automatically sucked into the test liquid to start detection. The device needs to be subjected to multiple operation steps such as sampling, mixing and detecting, the reaction time needs to be counted down by starting a key by a user, the operation process is complex, and the read result may generate errors of the reaction time due to incorrect manual operation.

Therefore, it is needed to provide a saliva detecting device capable of automatically completing sampling, detecting and timing and having functions of prompting, lighting and the like, so as to further improve the accuracy and sensitivity of saliva detection.

Disclosure of Invention

The invention provides a saliva detecting pen and a using method thereof, wherein the detecting pen comprises a circuit device, a liquid sucking rod, a test paper strip and a control chip, the circuit device comprises a circuit line and an indicator lamp, the liquid sucking rod comprises a liquid sucking end and a conveying end, and the conveying end is simultaneously connected with a positive electrode, a negative electrode and the test paper strip of the circuit line. In the detection process, the sample is transferred from the liquid suction end to the conveying end, the circuit is communicated, and the indicator light is lighted to prompt that the sample is sufficiently collected and the reaction is started. The saliva detecting pen solves the problems of inaccurate reading time, insufficient light and the like, and improves the accuracy of saliva detection.

In the prior art, most of sampling and testing integrated detection devices, such as early pregnancy detection pens, fecal occult blood detection tubes, saliva drug detection pens, urine drug detection cups and the like, reduce operation steps, and facilitate operation of terminal clients and obtain accurate results. However, the results are often abnormal or deviated due to insufficient sampling (particularly saliva products) or factors such as no clear prompt when the reading time is not strictly followed, and the reading results are incorrect due to large difference of reading environments. For example, in drug detection, a police provides a detection pen to detect suspected objects at night, and because light is darker and time is often urgent, the saliva detection pen is required to have obvious light indication in the detection process, the detection process of the detection pen is reminded by the light, detection is completed as soon as possible, and a final detection result is given.

According to the characteristics of the existing immunochromatography product, a circuit device, a liquid suction rod, a test strip and a control chip are designed. The pilot lamp in the circuit device can indicate the reaction to start to set up positive, negative electrode at the imbibition stick delivery end, after accomplishing the imbibition, positive, negative electrode intercommunication begins to start the pilot lamp scintillation flow, and control chip has preset the reaction time of pilot lamp scintillation, generally 4min, and after reaction time reached 4min, the pilot lamp is long bright, indicates that can read.

However, in the practical use process, it is often found that due to the influence of factors such as the material and the aperture of the liquid absorbing rod, the flowing speed of the sample in the liquid absorbing rod may be inconsistent, so that some samples arrive at the test strip to start the reaction, and some samples arrive at the liquid absorbing rod to react, so that when the reaction time reaches 4min, the samples arrive at the reaction, the reaction is not completed, and the detection result is negative or weak positive. Of course, this problem can also be solved by extending the reaction time in the detection, but this brings about the cost of time wasted for more testers and testers to wait together, and the problem brought by the detection apparatus itself cannot be solved by extending the reaction time.

Therefore, key factors such as the material of the liquid absorbing rod, the electrode material, the electrode distance and the like need to be determined, the flow consistency of the sample in the liquid absorbing rod is accurately controlled, meanwhile, the sample reaches the test strip to start reaction, and positive and negative electrodes also need to sensitively react the process of making a circuit be switched on after the sample reaches the liquid absorbing rod, so that the sensitivity and the accuracy of a detection result are improved.

In one aspect, the invention provides a saliva detection pen, which comprises a circuit device, a liquid suction rod and a test strip, wherein the circuit device comprises a circuit line and an indicator light.

In the existing saliva detection technology, saliva sampling detection analysis is generally carried out by only adopting saliva detection test paper or a detection pen with a liquid suction piece and detection test paper, wherein one end of the liquid suction piece of the detection pen is connected with the detection test paper, and the result is abnormal or deviated due to no clear prompt, and the reading result is incorrect due to large difference of reading environments. In the detection pen provided by the invention, the conveying end of the liquid suction rod is simultaneously connected with the circuit device and the test paper strip. In addition, because the bottom of the test strip and the delivery end of the liquid suction rod are partially overlapped, the sample simultaneously enters the test strip, and the reaction starts simultaneously when the indicator lights are lighted. Compared with the prior art, the indicator lamp device is additionally arranged, and the detection process can be marked.

Further, the liquid sucking rod comprises a liquid sucking end and a conveying end, wherein the liquid sucking end is used for contacting and sucking a sample, the conveying end is used for conveying the sample to the test strip, and the conveying end is connected with the positive electrode and the negative electrode of the circuit line.

The liquid suction rod provided by the invention comprises the liquid suction end and the conveying end, when a sample is migrated from the liquid suction end to the conveying end, the indicator light starts to flash to prompt when a circuit is communicated, so that the sample can be fully collected, the sample is stopped from entering the test strip to participate in detection and analysis, and the data result is reliable and accurate. In the collecting and detecting process, the indicator lamp prompts that the sample collection is completed, the sample collection can be finished, the sample enters the analysis, the subsequent reaction process is carried out through the reaction time set by the control chip, and the accuracy of the detection result is improved. In addition, the liquid absorbing rod provided by the invention adopts a biocompatible material and is sterilized, compared with the method of dipping a saliva sample and then analyzing on a test strip in the prior art, the liquid absorbing rod can directly absorb the saliva sample in the mouth, so that the sample collecting and analyzing steps are simplified, the practical use process is more convenient, and the operation is simpler.

Further, the liquid absorbing rod is porous fiber, the porous fiber material comprises one or more of polyolefin, polyester and polyamide, and the pore size is 5-10 mu m.

In some modes, the liquid absorbing rod provided by the invention is porous fiber, and the test paper strip material is nitrocellulose membrane.

The chemical properties of the porous fiber are determined by its material, and the selection of a suitable polymer material is important to ensure the durable function of the saliva test pen and the accuracy of the test result, and the material of the porous fiber is usually selected by the chemical substance in contact with it. The invention compares and analyzes the accuracy of results when polyolefin, polyester, polyamide and other different porous fiber materials are used for collecting and analyzing morphine and other fat-soluble drugs, and discovers that the polyolefin material has good water absorption capacity and high sensitivity compared with other materials, and the other materials can cause uneven sample absorption and inaccurate results when being detected.

Also, the pore size of the porous fiber can be large or small depending on the material, which directly affects the resolution of the analysis, the sample flow rate, and the sensitivity of the test. By selecting porous fibers with proper pore sizes, the liquid suction speed and the filtering efficiency of the liquid suction rod can be better controlled. Therefore, porous fibers with different pore sizes are further screened by using morphine standard, and the result shows that the pore size of the porous fibers suitable for morphine drug detection is 10 mu m.

Further, the electrode materials of the positive electrode and the negative electrode comprise any one or more of gold, platinum, copper and iron.

Since the communication of the circuit means is initiated when the chemical reaction occurs when the sample liquid migrates to the delivery end, the selection of a suitable electrode material is necessary to increase the sensitivity of the reaction. Electrode materials suitable for use in chemical reactions need to take into account a number of factors such as chemical stability, electrochemical activity, and electrical conductivity. According to the invention, through screening experiments, the platinum as the metal electrode material has excellent electrochemical activity, chemical stability and conductivity, and can be used for obviously improving the sensitivity and accuracy of reaction detection.

Further, the distance between the positive electrode and the negative electrode is 5-10 mm.

When the sample is fully transferred to the conveying end, the circuit device is communicated, the indicator lamp starts to light to prompt, and in the previous process, the transfer speed and the adsorption quantity of the sample on the liquid suction rod are particularly critical. Besides the material and aperture of the liquid suction rod can influence the adsorption capacity of the sample, the distance between the positive electrode and the negative electrode (namely the electrode distance) of the conveying end of the liquid suction rod can influence the final detection result. Too large electrode distance can lead to the situation that an excessive sample needs to be collected to be communicated with a circuit, the flickering time of an indicator lamp is delayed from the reaction time, so that the reaction is insufficient, and too small electrode distance can lead to insufficient sample collection, the reaction starts in advance, and the detection result can be falsely detected as weak positive or negative by a positive result.

In some embodiments, the distance between the positive and negative electrodes is 5mm.

Further, the detection pen also comprises a control chip and a shell, wherein the control chip is positioned on one side of the liquid suction rod, and the shell comprises a shell and a pen cap.

The saliva detecting pen provided by the invention is also provided with the control chip, so that the reaction time can be set, when the indicator lamp prompts the reaction to start, the chip enters the countdown mode until the reaction is finished, and the reading error caused by manual timing or visual observation is avoided.

In some embodiments, the control chip sets the reaction time to 4 minutes.

In some modes, a shell and a pen cap are sleeved outside the circuit device, the liquid suction rod, the test paper strip and the control chip, the shell comprises a first shell and a second shell, and an observation window is arranged on the first shell and used for observing a recording result. The pen cap is used for protecting the liquid suction end of the liquid suction rod, a diaphragm is arranged in the pen cap, and when the pen cap is taken off, the control chip controls the indicator lamp to flash once to prompt the detection pen to be in a normal state.

In another aspect, the invention provides a saliva test method, characterized in that the test is performed using a saliva test pen as described above.

Further, the method comprises the steps of:

(1) The pen cap is taken down, and the indicator light is turned on to prompt that the detection pen is in an effective state, so that the pen cap can be used;

(2) The liquid suction end of the liquid suction rod adsorbs a sample, liquid migration and flow reach the conveying end, a circuit line is communicated, an indicator light is lighted to prompt that the sample is sufficiently collected, and the sampling is stopped;

(4) The indicator lamp is kept normally on, the completion of the reaction is prompted, the detection is completed, and the result is recorded.

Further, the indication lamp in the steps (1) and (2) is turned on in a flashing manner.

In some modes, the pen cap is taken down, the indicator lamp flashes once to prompt a normal state, if the indicator lamp is abnormal, the detection pen needs to be checked, when the sample liquid reaches the conveying end, the circuit is communicated, the indicator lamp starts to flash continuously to prompt the reaction to start, and when the reaction is finished, the indicator lamp stops flashing, keeps always bright, provides a certain light for result record, and improves the accuracy of reading.

In still another aspect, the invention provides an application of a detection pen in preparing a device for improving saliva detection accuracy, wherein the device comprises a circuit device, a liquid suction rod, a test strip, a control chip and a shell, and the circuit device comprises a circuit line and an indicator lamp.

The detection pen provided by the invention is provided with the indicator lamp and the control chip, can accurately control the reaction time and provide illumination light for result reading, simultaneously screens the material, the aperture, the electrode material and the like of the liquid suction rod, provides a device which is most suitable for reaction detection according to the detection purpose, and improves the accuracy and the sensitivity of saliva detection analysis.

Compared with the prior art, the invention has the following advantages:

1. The saliva detection pen provided by the invention is provided with the indicator lamp, the reaction process can be clarified through the prompt of the indicator lamp, and the reading accuracy in a dim environment is improved;

2. The porous fiber is used as a morphine drug imbibition rod material, and metal platinum is used as an electrode material, so that the flow rate of a sample and the amount of the sample required for subsequent reaction are accurately controlled, and the detection accuracy and sensitivity are improved;

3. the method is simple to use, and improves the effectiveness and application range of morphine drug detection.

Drawings

FIG. 1 is a schematic view showing the appearance of a saliva detecting pen according to example 1;

FIG. 2 is a schematic diagram showing the split structure of the saliva detecting pen of example 1;

fig. 3 is a schematic sectional view of a saliva detecting pen according to example 1.

Detailed Description

The following description of the preferred embodiments of the present invention is further detailed in conjunction with the accompanying drawings, and it should be noted that the embodiments described below are intended to facilitate an understanding of the present invention, and are not intended to limit the invention in any way. The materials and equipment used in the embodiments of the present invention are all known products and are obtained by purchasing commercially available products.

The following preferred embodiments of the present invention are provided in no way to limit the invention, but to enable any modification, equivalent replacement, improvement or the like to be made within the spirit and principles of the present invention.

Example 1 saliva test pen provided by the invention

The saliva detecting pen provided in this embodiment is shown in fig. 1-3, in which fig. 1 is a schematic view of an appearance structure of the saliva detecting pen, fig. 2 is a schematic view of a split structure of the saliva detecting pen, and fig. 3 is a schematic view of a cross-sectional structure of the saliva detecting pen.

As shown in fig. 1-3, the saliva detecting pen 1 provided by the invention comprises a circuit device 2, a liquid absorbing rod 3, a test strip 4, a control chip 5 and a shell 6, wherein the shell 6 comprises a shell 61 and a pen cap 62, the shell 61 comprises a first shell 611 and a second shell 612, and an observation window 6111 is arranged on the first shell 611.

The circuit device 2 comprises a circuit line 21 and an indicator light 22, wherein the two ends of the liquid suction rod 31 are respectively provided with a liquid suction end 311 and a conveying end 312, the liquid suction rod 31 is made of porous fiber, the material is polyolefin, and the pore size is 10 mu m. Both ends of the delivery end 312 of the liquid suction rod 31 are respectively connected with the positive electrode 211 and the negative electrode 212 of the circuit line 21, the two electrodes are made of metal platinum, and the electrode distance is 5mm. The indicator light 22 and the control chip 5 are connected to the circuit arrangement 2 via a circuit line 21, the control chip 5 being located on one side of the wick 3. The inner side of the pen cap 62 is covered with a diaphragm 621, and the control chip 5 senses the control indicator lamp through the diaphragm 621 to display and detect the pen state.

Example 2 saliva test pen and method of Using the same

The saliva detection pen provided in the embodiment 1 is adopted to collect, detect and analyze drugs in saliva samples, and the specific operation steps are as follows:

(1) The pen cap is taken down, the indicator lamp flashes once to indicate that the detection pen is in an effective state, and the detection pen can be used for saliva detection and analysis;

(2) The liquid suction end of the liquid suction rod collects samples, liquid flows and migrates to the conveying end of the liquid suction rod, the liquid is communicated with the circuit, the indicator lights start to flash continuously, the samples are prompted to be sufficiently collected and react, the reaction time is 4min, and the sampling is stopped;

(3) The indicator light stops flashing and keeps normally bright, the reaction is prompted to finish, and detection is completed;

(4) The detection result on the test strip is recorded through the observation window.

Example 2 screening of porous fiber Material

The porous fibers used in the prior art generally include polyolefins, polyesters, polyamides, and biodegradable materials such as cellulose acetate. The porous fibers of different materials have different chemical properties, so that the porous fibers have different drug absorption capacity, hydrophilicity and the like in saliva samples. For saliva sample adsorption, especially adsorption materials capable of improving accuracy and sensitivity of morphine drug detection, three porous fiber materials shown in table 1 are used for preparing saliva detection pens in the embodiment, other structures are the same as those of embodiment 1, detection analysis is carried out on 200 μl morphine standard sample with concentrations of 0ng/ml, 100ng/ml, 200ng/ml and 300ng/ml respectively according to the detection method described in embodiment 2, and 3 repetitions are set for each concentration to screen porous fiber materials suitable for morphine drug adsorption detection. The test results are shown in Table 1.

TABLE 1 detection results of liquid suction bars of different materials

Note that "+" indicates positive, "±" indicates weak positive, and "-" indicates negative.

According to Table 2, it can be seen that when the saliva test pen prepared from three materials is used for testing under the condition of low concentration (0 ng/ml and 100 ng/ml), the consistency of the test result of the polyester material is poor, a weak positive result appears under the condition of 0ng/ml, a negative result appears under the condition of 100ng/ml, and when the saliva test pen is used for testing under the condition of high concentration (200 ng/ml and 300 ng/ml), the test results of the polyester and the polyamide are both weak positive results, the repeatability is poor, the test accuracy is 50% -100%, and the error of the test results is possibly caused by poor adsorption of the material to the drug when the polyester or the polyamide adsorbs the morphine standard, uneven biomolecule flow or partial blockage exists in the sample, the sample quantity is insufficient when the circuit is communicated, the indicator lamp starts timing in advance, and the sample reacted in the reaction time of 4min is insufficient, and the error occurs in the test results. When the saliva detecting pen prepared from the polyolefin material detects morphine drugs, the accuracy is 100%, and the repeatability of the detection result is consistent no matter in a low-concentration sample or a high-concentration sample, so that the sensitivity and the detection accuracy of the saliva detecting pen are obviously improved by the polyolefin material, and the porous fiber liquid absorbing rod of the polyolefin material is preferred.

Example 3 pore size screening of porous fibers

The porous fiber is used as an adsorption carrier of saliva samples, and the selection of the pore size directly influences the flow speed of detection samples and the sensitivity of analysis. Larger pore sizes may allow easier passage of these biological macromolecules, but may result in an excessive amount of sample undergoing the reaction, while insufficient sample reaction occurs for a limited period of time, while smaller pore sizes may increase binding specificity, but may limit passage of analytes, resulting in insufficient sample for subsequent reactions.

In order to screen the pore size of the porous fiber suitable for the collection, detection and analysis of morphine in saliva samples, 200 μl of morphine with concentrations of 0ng/ml, 100ng/ml, 300ng/ml and 500ng/ml are used as standard substances in the embodiment, and the saliva detection pen provided in the embodiment 1 is used, wherein the pore size of the porous fiber is shown in table 2, and the detection result is shown in table 2 according to the use method described in the embodiment 2.

TABLE 2 detection results of porous fibers of different pore sizes

As can be seen from Table 2, when the saliva detecting pen prepared by the porous fiber with the pore size of 5 μm is used for morphine drug detection, the accuracy is 25% -50%, and when the concentration of the standard substance is higher, the repeatability of the detection result is poorer, the accuracy is lower, the substances in the standard substance are blocked due to the fact that the pore size of the porous fiber is smaller, and the substances contained in the standard substance are more higher, so that the detection result is more serious, the detection result is inaccurate, when the pore size of the porous fiber is larger than or equal to 7 μm, the repeatability and the accuracy of the detection result are improved, wherein when the pore size is equal to 10 μm, the accuracy of the detection result is 100%, the repeatability is good, and when the pore size is 12 μm, the biomolecule flow rate in morphine is too high, the sample for reaction is too much, the reaction is insufficient, and the detection result is weak positive. As a result, it was found that too small a pore size of the membrane may cause clogging or uneven flow of the liquid, and too large a pore size may cause too many samples to be reacted, resulting in insufficient reaction, so that the porous fiber having a pore size of 10 μm is preferable in this example.

Example 4 screening of electrode Material

The electrode materials suitable for chemical reaction need to consider a plurality of factors such as chemical stability, electrochemical activity and conductivity, and different electrode materials can cause the unstable problem of contact and insensitive response of the circuit device due to different materials, so that deviation or abnormality of response time of the indicator lamp is caused, and further the detection result is unreliable.

Therefore, in order to screen electrode materials suitable for both ends of the circuit line to improve the sensitivity of saliva detection process and the accuracy of the result, the present example provides 4 electrode material preparation saliva detection pens as shown in table 3, other structures are the same as example 1, according to the detection method described in example 2, 20 parts of morphine standard (100 ng/ml to 300 ng/ml) with different concentrations are analyzed at 200 μl per part, the detection result and the indicator lamp start blinking time are recorded, and the results are shown in the following table.

TABLE 3 detection of different cathode and anode materials

As is clear from the results of table 3, when different metals were used as the electrode materials, there was a difference between the sensitivity of the circuit device and the sampling detection result of the detection pen. When iron and gold are used as electrode materials, the indicator lamp starts to flash with a lag time, the accuracy of detection results is 70-75%, probably due to poor conductivity of the two metal materials in morphine drug solution, when sample flows to a conveying end to start reaction, a circuit device is not timely communicated, the indicator lamp does not indicate that reaction starts, excessive sample is adsorbed by a liquid absorbing end, subsequent reaction is insufficient, weak positive or negative detection results appear, when copper is used as the metal materials, the detection accuracy is 85%, the indicator lamp flashes in advance, weak positive detection results appear, probably due to the fact that when copper is used as the electrode materials in morphine solution, the electrochemical activity is poor, the connecting circuit device is not stable enough, the response time of the indicator lamp is inaccurate, and deviation appears in detection results, and when platinum sheet is used as the electrode materials, the stability of the circuit device is remarkably improved, and the accuracy of detection results can reach 95%, so that in the embodiment, platinum is preferably used as the electrode materials.

Example 5 screening of electrode distance

In order to further analyze whether the interface distances of the electrodes at the two ends of the circuit line have an influence on the final detection result, in this embodiment, 200 μl of morphine standard substances (100 ng/ml to 300 ng/ml) with different concentrations is taken as a sample, and according to the saliva detection pen provided in embodiment 1, the electrode distances are set to 2.5mm, 5mm, 10mm and 12.5mm, and according to the detection method in embodiment 2, the detection effects of the saliva detection pen under the four electrode distances are compared and analyzed, and the results are shown in table 4.

TABLE 4 Effect of different electrode distances on detection results of Morphorphine Standard

As shown in Table 4, the morphine standard with different concentrations can be accurately detected when the electrode distance is 5mm, and the indicator lights normally flash, and when the electrode distance is equal to 7.5mm or 10mm, the detection of the morphine standard is reduced to 85%, the flashing time of the indicator lights is delayed, the result is inaccurate because the electrode distance is too large, so that the samples required to be adsorbed by the communication circuit are insufficient, the detection result is inaccurate when the electrode distance is 2.5mm, the accuracy is 80%, negative results appear, the circuit is communicated when a small amount of samples flow to the conveying end, the indicator lights flash in advance, the reaction starts in advance, and part of the samples do not reach the conveying end to start reacting, so that the result is inaccurate. According to the above analysis, the present embodiment prefers an electrode distance of 5 mm.

The foregoing is merely illustrative of specific embodiments of the present invention, but the design concept of the present invention is not limited thereto, and any insubstantial modification of the present invention by using the design concept shall fall within the scope of the present invention.

Claims

1. The saliva detecting pen is characterized by comprising a circuit device, a liquid sucking rod and a test strip, wherein the circuit device comprises a circuit line and an indicator lamp.

2. The test pen of claim 1, wherein the pipette tip comprises a pipette tip for contacting and absorbing a sample and a delivery tip for delivering the sample to a test strip, the delivery tip being connected to the positive and negative electrodes of the circuit line.

3. The detecting pen as claimed in claim 2, wherein the liquid absorbing rod is porous fiber, and the porous fiber material includes one or more of polyolefin, polyester and polyamide, and has a pore size of 5-10 μm.

4. The test pencil of claim 3 wherein the electrode material of the positive and negative electrodes comprises any one or more of gold, platinum, copper, iron.

5. The test pencil of claim 4, wherein the distance between the positive and negative electrodes is 5-10 mm.

6. The pen of claim 5, further comprising a control chip and a housing, the control chip being located on one side of the wand, the housing comprising a housing and a cap.

7. A saliva testing method, characterized in that it is carried out with a saliva testing pen according to claims 1-6.

8. The method of claim 7, comprising the steps of:

(2) The liquid suction end of the liquid suction rod adsorbs a sample, the liquid migration flow reaches the conveying end, the circuit line is communicated, the indicator light is lighted to prompt that the sample is sufficiently collected and starts to react, and the sampling is stopped;

(4) The chip controls the reaction time, the reaction ending indicator lamp keeps normally on, the completion of detection is prompted, and the result is recorded.

9. The method of claim 8, wherein the indication lamp in step (1) and (2) is lighted by flashing.

10. The application of the detection pen in preparing a device for improving saliva detection accuracy is characterized in that the device comprises a circuit device, a liquid suction rod, a test paper strip, a control chip and a shell, and the circuit device comprises a circuit line and an indicator lamp.