CN119199107B - Quantitative detection device for fluorescence immunochromatography - Google Patents
Quantitative detection device for fluorescence immunochromatography Download PDFInfo
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- CN119199107B CN119199107B CN202411709697.XA CN202411709697A CN119199107B CN 119199107 B CN119199107 B CN 119199107B CN 202411709697 A CN202411709697 A CN 202411709697A CN 119199107 B CN119199107 B CN 119199107B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
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Abstract
The invention relates to the technical field of immunochromatography detection, and discloses a quantitative detection device for fluorescence immunochromatography, which comprises a sample adding module, a fluorescence detection module, a temperature control module and a conveying module, wherein the sample adding module, the fluorescence detection module, the temperature control module and the conveying module are all arranged in a case, the conveying module comprises a first conveying part, the first conveying part carries linearly, the temperature control module comprises a copper pipe, the copper pipe is provided with a straight heat exchange part, and detection samples are rapidly controlled to the temperature required by experiments through mutual coordination among the sample adding module, the fluorescence detection module, the temperature control module and the conveying module, so that the interference of the temperature on the quantitative detection of the fluorescence immunochromatography can be effectively reduced, the accuracy of detection results can be ensured, the detection precision can be improved, and meanwhile, the automation degree of the whole equipment is high, and the operation difficulty can be further reduced.
Description
Technical Field
The invention relates to the technical field of immunochromatography detection, in particular to a quantitative detection device for fluorescence immunochromatography.
Background
Oxidized low density lipoprotein (oxLDL) is the product of oxidation of Low Density Lipoprotein (LDL) in vivo, and has a close relationship with atherosclerosis and the development of cardiovascular diseases. Oxidized low density lipoprotein can be quantitatively detected by fluorescent immunochromatography. When in quantitative analysis, an operator drops a blood sample onto the reagent card, the reagent card is put into an analysis instrument after a certain time interval, and the quantitative result is read by the instrument.
However, the existing fluorescence immunochromatography quantitative detection equipment is easily affected by temperature, light and the like, external light can directly interfere with the analysis result of an analysis instrument when the analysis instrument analyzes fluorescence, the temperature can not only cause the change of the conformation and spectral properties of fluorescent molecules, but also cause nonspecific binding to different degrees, especially near the room temperature of a human body, under the temperature environment, the activity of protein is high, specific and nonspecific binding are easy to occur, the analysis result can be seriously affected, and especially in some weak positive detection, the influence of the temperature can easily cause misjudgment of the detection result.
Disclosure of Invention
The invention aims to provide a quantitative detection device for fluorescence immunochromatography, which aims to solve the problem that the existing fluorescence immunochromatography equipment is easily interfered so as to influence the detection result.
The invention is realized by the following technical scheme:
The utility model provides a fluorescence immunochromatography's quantitative determination device, includes the application of sample module, fluorescence detection module, temperature control module and the transport module of locating quick-witted incasement, its characterized in that, transport module includes first transport portion, be fixed with a plurality of sample heat transfer module on the first transport portion, temperature control module includes the copper pipe, be equipped with straight heat exchange portion on the copper pipe, one side of conveyer belt is arranged in two in the first transport portion between the heat exchange portion, heat exchange portion with the direction of delivery of first transport portion is parallel, transport module still includes second transport portion, the second transport portion is lifter region and horizontal sideslip district, the top of lifter region is reagent card drop sample department, drops the operation on the reagent card in this reagent card drop sample department, be equipped with fluorescence detection department on the horizontal sideslip district, fluorescence detection department top is equipped with fluorescence detection module.
In one possible design, the sample heat exchange assembly comprises a first heat exchange plate, a second heat exchange plate and a disposable heat exchange tube, wherein the first heat exchange plate and the second heat exchange plate are detachably fixed, and a part of the disposable heat exchange tube is arranged between the first heat exchange plate and the second heat exchange plate in a bending state.
In one possible design, one end of the disposable heat exchange tube is fixed with a sample storage cup, the inside of the sample storage cup is communicated with the inside of the disposable heat exchange tube, a placement seat is fixedly arranged on the first heat exchange plate and used for placing the sample storage cup, the other end of the disposable heat exchange tube vertically penetrates through the second heat exchange plate downwards, and when the sample storage cup is placed on the placement seat, the liquid level of a sample dropped in the sample storage cup is not more than the highest position of the bent position of the disposable heat exchange tube at most.
In one possible design, the sample storage cup is fixedly provided with a fixing plate, the chassis is internally provided with a lifting control mechanism, and the lifting control mechanism can lift the sample storage cup by lifting the fixing plate after contacting with the fixing plate.
In one possible design, the first conveying section comprises a conveyor belt, which is controlled by a drive motor.
In one possible design, the first conveying part includes two identical conveyer belts, two first installation buckles are fixed on the conveyer belts, two second installation buckles are fixed on each sample heat exchange component, the second installation buckles at the upper end of the same sample heat exchange component are fixed with the first installation buckles on the conveyer belt, the second installation buckles at the lower end are fixed with the first installation buckles on the conveyer belt, and the two first installation buckles fixed with the same sample heat exchange component are positioned at the same vertical position, a central hole is formed in the center of the first installation buckles, a first magnet is fixed in the central hole, a central bulge matched with the central hole is arranged on the second installation buckles, and a second magnet is fixed on the central bulge.
In one possible design, the second conveying part includes a reagent card placing groove and conveying belt groups arranged on two sides of the reagent card placing groove, the conveying belt groups are symmetrically arranged with the reagent card placing groove as a center, each conveying belt group includes a conveying belt and a plurality of belt wheels, and the plurality of belt wheels are connected and driven by one conveying belt.
In one possible design, two connecting columns are fixed at two ends of the reagent card placing groove, each end of the reagent card placing groove is fixedly connected with the conveying belt through the connecting column, an observation window and a bayonet opening formed in one side of the reagent card placing groove are formed in the reagent card placing groove, the observation window is used for observing a C/T line of a reagent card, and the bayonet opening is used for inserting the reagent card into the reagent card placing groove.
In one possible design, the reagent card drop sample position and the fluorescence detection position are both fixed with a temperature control plate, when the conveyer belt conveys the reagent card placing groove to the temperature control plate, the temperature control plate can enable the reagent card placing groove and the reagent card fixed on the reagent card placing groove to keep a horizontal state, a waste reagent card temporary storage area is arranged in the lower position of the card inserting window in the case, used waste reagent cards are stored in the waste reagent card temporary storage area, when the reagent card placing groove on the conveyer belt moves from a position close to the waste reagent card temporary storage area to a card inserting window position, a card inserting opening of the reagent card placing groove faces downwards, and when the reagent card placing groove runs along a route from the card inserting window-the reagent card drop sample position-the fluorescence detection position-the waste reagent card temporary storage area, the card placing groove only faces to two horizontal sides and vertical upwards directions.
In one possible design, the top of the case is provided with a sample dropping port, a cover plate is arranged at the sample dropping port, one part of the cover plate is hinged on the case, a sealing ring is fixed on the cover plate, a switch is also fixedly arranged at the hinged position of the cover plate and the case, the switch is in a bouncing state when the cover plate is opened, and the switch is in a pressing state when the cover plate is closed.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. According to the invention, the sample adding module, the fluorescence detection module, the temperature control module and the conveying module are matched with each other to rapidly control the detection sample to the temperature required by an experiment, so that the interference of the temperature on quantitative detection of fluorescence immunochromatography can be effectively reduced, the accuracy of a detection result is ensured, the detection precision is improved, and meanwhile, the operation difficulty can be further reduced due to high automation degree of the whole equipment.
2. The case can realize the full sealing of the sample adding module, the fluorescent detection module, the temperature control module and the conveying module, can preserve heat of the detection environment in the case, and can prevent an external light source from entering the case to interfere the measurement of the fluorescent detection module.
Drawings
The accompanying drawings, which are included to provide a further understanding of embodiments of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the principles of the application. In the drawings:
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a side view of the present invention;
FIG. 3 is a cross-sectional view taken at A-A of FIG. 2 in accordance with the present invention;
FIG. 4 is an enlarged view of the invention at B in FIG. 3;
FIG. 5 is an enlarged view of the invention at C in FIG. 3;
FIG. 6 is a schematic diagram of a connection between a reagent card slot and a conveyor belt according to an embodiment of the present invention;
FIG. 7 is a half-sectional view of a sample heat exchange assembly according to an embodiment of the present invention;
FIG. 8 is a top view of a sample storage cup according to an embodiment of the present invention;
FIG. 9 is a schematic view of the connection members between the conveyor belt and the sample heat exchange assembly in an embodiment of the invention.
The reference numerals represent 1-machine case, 2-operation panel, 3-cover plate, 4-copper pipe, 5-reagent card placing groove, 501-connecting column, 502-observation window, 503-bayonet, 6-conveyer belt, 7-belt wheel, 8-waste reagent card temporary storage area, 9-fluorescence detection module, 10-sample heat exchange component, 101-first heat exchange plate, 1011-placing seat, 102-second heat exchange plate, 11-conveyer belt, 12-lifting control mechanism, 13-temperature control plate, 14-switch, 15-disposable heat exchange tube, 16-sample storage cup, 1601-fixing sheet, 17-window sealing cover, 18-suction magnet, 19-trapezoid block, 20-first mounting buckle, 2001-first magnet, 21-second mounting buckle and 2101-second magnet.
Detailed Description
For the purpose of making apparent the objects, technical solutions and advantages of the present invention, the present invention will be further described in detail with reference to the following examples and the accompanying drawings, wherein the exemplary embodiments of the present invention and the descriptions thereof are for illustrating the present invention only and are not to be construed as limiting the present invention.
The embodiment, as shown in fig. 1 to 9, includes a sample loading module, a fluorescence detection module 9, a temperature control module and a conveying module, where the sample loading module, the fluorescence detection module 9, the temperature control module and the conveying module are all disposed in a chassis 1, the conveying module includes a first conveying portion, a plurality of sample heat exchange assemblies 10 are fixed on the first conveying portion, the first conveying portion is a linear conveying, the temperature control module includes a copper pipe 4, a straight heat exchange portion is disposed on the copper pipe 4, one side of a conveyor belt 11 in the first conveying portion is disposed between two heat exchange portions, and when the sample heat exchange assemblies 10 fixed on the first conveying portion are moved to the side, the temperature of the copper pipe 4 can quickly cause the temperature change of the sample heat exchange assemblies 10, and the heat exchange portion is parallel to the conveying direction of the first conveying portion.
The conveying module further comprises a second conveying part, the second conveying part comprises a lifting area and a horizontal traversing area, the top end of the lifting area is a reagent card sample dripping position, the operation of dripping a sample onto the reagent card is completed at the reagent card sample dripping position, a fluorescence detection position is arranged on the horizontal traversing area, a fluorescence detection module 9 is arranged above the fluorescence detection position, when the reagent card waits for a period of reaction time after finishing sample dripping at the reagent card sample dripping position, the reagent card is conveyed to the fluorescence detection position through the second conveying part after the reaction is completed, and the detection is completed through the fluorescence detection module 9.
It should be noted that, the fluorescence detection module 9 preferably uses a fluorescence reader, uses the fluorescence reader to read the fluorescence intensities of the detection line and the control line and calculate the T/C value, and calculates the content of the specific substance in the sample through the standard curve built in the instrument, so as to ensure the accuracy of the detection result.
Further, the sample heat exchange assembly 10 includes a first heat exchange plate 101, a second heat exchange plate 102, and a disposable heat exchange tube 15, where the first heat exchange plate 101 and the second heat exchange plate 102 are detachably fixed, a portion of the disposable heat exchange tube 15 is in a bent state and is disposed between the first heat exchange plate 101 and the second heat exchange plate 102, one end of the disposable heat exchange tube 15 is fixed with a sample storage cup 16, the inside of the sample storage cup 16 is communicated with the inside of the disposable heat exchange tube 15, a placement seat 1011 is fixedly disposed on the first heat exchange plate 101, the placement seat 1011 is used for placing the sample storage cup 16, the other end of the disposable heat exchange tube 15 vertically penetrates through the second heat exchange plate 102 downwards, when the sample storage cup 16 is placed on the placement seat 1011, the liquid level of a sample dropped into the sample storage cup 16 does not exceed the highest position where the disposable heat exchange tube 15 is bent, at this time, even if the sample storage cup 16 is filled with sample liquid, the sample liquid will not flow out from the other end of the disposable heat exchange tube 15 to the reagent card, after the sample is dripped into the sample storage cup 16, the sample storage cup 16 is lifted up, so that the height of the sample storage cup 16 is higher than the highest position of the bending position of the disposable heat exchange tube 15, the sample liquid in the sample storage cup 16 can be dripped onto the sample card from the other end of the disposable heat exchange tube 15, wherein the disposable heat exchange tube 15 is made into a tube shape with a small diameter and a long length, the heat exchange area between the disposable heat exchange tube 15 and the heat exchange plate can be increased, the diameter of the disposable heat exchange tube 15 is smaller than 1 millimeter, and when the sample liquid flows from the bending position of the sample storage cup 16 to the other end through the bending position of the disposable heat exchange tube 15, the sample liquid can flow into the copper tube 4, the first heat exchange plate 101 and the second heat exchange plate 102 exchange heat rapidly, and the disposable heat exchange tube 15 reaches the temperature required for detection reaction from the sample storage cup 16.
Still further, the sample storage cup 16 is fixedly provided with a fixing piece 1601, the chassis 1 is internally provided with a lifting control mechanism 12, the lifting control mechanism 12 comprises a claw with a U-shaped groove, the claw can lift the sample storage cup 16 by lifting the fixing piece 1601 after contacting with the fixing piece 1601, so that automatic sample dripping is realized, and when the claw is lifted to be higher than the sample heat exchange assembly 10, the lifting control mechanism 12 cannot interfere with the operation of the first conveying part as a whole.
Meanwhile, the first conveying part comprises the conveying belt 11, the conveying belt 11 is controlled by a driving motor, a plurality of sample heat exchange assemblies 10 can be fixed on the conveying belt 11, temperature treatment of a plurality of samples can be rapidly and continuously completed through the plurality of sample heat exchange assemblies 10, and when a detection sample is conveyed by the first conveying part, the heat exchange part can rapidly exchange heat of the sample heat exchange assemblies 10 to a required detection temperature, so that rapid temperature change of sample liquid to a temperature required by an experiment is further ensured before reaction with a reagent card, the experiment temperature is ensured, and non-specific combination during detection is reduced.
Further, the first conveying part includes two identical conveyor belts 11, two first mounting buckles 20 are fixed on the two conveyor belts 11, two second mounting buckles 21 are fixed on each sample heat exchange assembly 10, a central hole is formed in the center of each first mounting buckle 20, a central protrusion matched with the central hole is formed in each second mounting buckle 21, a second magnet 2101 is fixedly arranged on each central protrusion, the second mounting buckle 21 at the lower end is fixed with the first mounting buckle 20 on the conveyor belt 11, the two first mounting buckles 20 fixed with the same sample heat exchange assembly 10 are located at the same vertical position, a central hole is formed in the center of each first mounting buckle 20, a first magnet 2001 is fixed in each central hole, a second magnet 2101 is fixedly arranged on each central protrusion, and the first mounting buckle 20 and the second mounting buckle 21 are fixed through mutual attraction between the first magnet 2001 and the second magnet 2101.
In this embodiment, the second conveying portion includes reagent card standing groove 5 and sets up conveyer belt 6 group in reagent card standing groove 5 both sides, conveyer belt 6 group with reagent card standing groove 5 is the central symmetry setting, every conveyer belt 6 group includes conveyer belt 6 and a plurality of band pulleys 7, and a plurality of band pulleys 7 are by conveyer belt 6 connection transmission, the both ends of reagent card standing groove 5 are fixed with two spliced poles 501, each end of reagent card standing groove 5 pass through this spliced pole 501 with conveyer belt 6 fixed connection, be equipped with reagent card standing groove on the reagent card standing groove 5, make reagent card and reagent card standing groove 5 fixed through placing reagent card in this reagent card standing groove, drive the reagent card through conveyer belt 6 and accomplish the removal of different positions to automatic completion is to the drop appearance of reagent card and the fluorescence measurement after the drop appearance, and degree of automation is high, simultaneously, can control the reaction time on the reagent card steadily and accurately after the drop appearance, effectively controls the time variable.
The reagent card drop sample position and the fluorescence detection position are both fixedly provided with the temperature control plate 13, when the conveyer belt 6 transports the reagent card placing groove 5 to the temperature control plate 13, the temperature control plate 13 can keep the reagent card placing groove 5 and the reagent card fixed on the reagent card placing groove 5 in a horizontal state, meanwhile, the temperature control plate 13 is internally provided with a temperature control pipe, and the temperature control pipe can further keep the reagent card at the temperature control plate 13 to a required test temperature.
At present, many detection devices for realizing quantitative detection by using immunochromatography have only one single reagent card placing groove 5, and the placing groove of a reagent card is inconvenient to use, mainly because the reagent card placing groove 5 is troublesome to install and take, further design is further carried out on the reagent card placing groove 5, referring to fig. 5 and 6, an observation window 502 and a card slot 503 formed at one side are formed on the reagent card placing groove 5, the observation window 502 is used for observing a C/T line of the reagent card, the card slot 503 is used for inserting a reagent card into the reagent card placing groove 5, a card inserting window is formed near a reagent card drop position on the side edge of the machine case 1, when the conveyer belt 6 drives the reagent card placing groove 5 to move to the card inserting window, the card slot 503 on the reagent card placing groove 5 is horizontally oriented to the card inserting window, at this moment, a C/T line of the reagent card is upwardly inserted into the reagent card placing groove 5 from the card inserting window, the card slot 503 is used for temporarily storing a reagent card in the machine case 1, a temporary storage area is automatically cleaned by using a temporary storage area 8 near a waste area 8 after the reagent card is placed in the machine case 1, the temporary storage area is automatically cleaned by using the temporary storage area 8 near the waste area 8 after the reagent card placing in the machine case 1, when the reagent card placing groove 5 runs along the route from the card inserting window to the reagent card sample dropping position to the fluorescence detection position to the abandoned reagent card temporary storage area 8, the directions of the card inserting openings 503 of the reagent card placing groove 5 only face the horizontal two sides and the vertical upward direction, and the reagent card can not fall from the reagent card placing groove 5, thus greatly facilitating card inserting operation and only needing to insert the reagent card.
Meanwhile, the main temperature control position of the copper pipe 4 is located at the heat exchange part, the reagent card drop sample and the fluorescence detection part, so that the energy consumption in temperature control is low, and the temperature environment can be changed very quickly after the heat exchange part is matched with the sample heat exchange assembly 10, so that the temperature variable in detection can be changed quickly and efficiently.
The card inserting window and the temporary storage area 8 of the waste reagent card are both provided with sealing plate assemblies, each sealing plate assembly comprises a window sealing cover 17, the upper end position of each window sealing cover 17 is hinged to the case 1, the lower end position of each window sealing cover 17 is fixedly provided with a suction magnet 18, the case 1 is also fixedly provided with the suction magnets 18, when the window sealing covers 17 are closed, the two suction magnets 18 are mutually attracted to enable the window sealing covers 17 to be closed more tightly, a trapezoid block 19 is further fixed above the hinged position of each window sealing cover 17, the trapezoid block 19 limits the window sealing covers 17, the window sealing covers 17 are prevented from rotating to a vertical state, and when an operator releases hands, the window sealing covers 17 can rotate downwards to close the window.
It should be noted that, the low temperature can reduce the non-specific binding in the detection process, the temperature range is 4-10 ℃, the binding between the antigen and the antibody is more stable in the environment, and more antigen-antibody complexes can be formed, which is particularly important for detecting weak positive samples, and the non-specific binding can be reduced, so that the copper tube 4 has the main function of rapid cooling.
In this embodiment, the top of machine case 1 is equipped with and drips the appearance mouth, it is equipped with apron 3 to drip appearance mouth department, apron 3 one is articulated on machine case 1, be fixed with the sealing washer on the apron 3, the sealing washer with apron 3 cooperation enable apron 3 with drip closely laminating between the appearance mouth to completely isolated machine case 1 makes machine case 1 not receive external light source interference, simultaneously, apron 3 can also play to the inside heat preservation effect of machine case 1.
Further, a switch 14 is further fixed at the hinge position of the cover plate 3 and the chassis 1, the switch 14 is in a sprung state when the cover plate 3 is opened, the switch 14 is in a pressed state when the cover plate 3 is closed, the opening and closing of the cover plate 3 can be judged through the opening and closing state of the switch 14, and meanwhile, whether the fluorescent detection module 9 can detect is judged through the opening and closing of the switch 14, so that the detection environment of the fluorescent detection module 9 is isolated from an external light source, and the accuracy of a detection result is ensured.
Further, the embodiment also comprises a data processing system, wherein the data processing system converts the fluorescence signal into a digital signal, and the concentration value is calculated by the data processing system.
The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the invention, and is not meant to limit the scope of the invention, but to limit the invention to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (7)
1. The quantitative detection device for the fluorescence immunochromatography comprises a sample adding module, a fluorescence detection module (9), a temperature control module and a conveying module which are arranged in a machine case (1), and is characterized in that the conveying module comprises a first conveying part, a plurality of sample heat exchange assemblies (10) are fixed on the first conveying part, the temperature control module comprises a copper pipe (4), a straight heat exchange part is arranged on the copper pipe (4), one side of a conveyor belt (11) in the first conveying part is arranged between the two heat exchange parts, and the heat exchange parts are parallel to the conveying direction of the first conveying part;
The conveying module further comprises a second conveying part, the second conveying part comprises a lifting area and a horizontal traversing area, the top end of the lifting area is a reagent card sample dripping position, the operation of dripping a sample onto a reagent card is completed at the reagent card sample dripping position, a fluorescence detection position is arranged on the horizontal traversing area, and a fluorescence detection module (9) is arranged above the fluorescence detection position;
the sample heat exchange device comprises a sample heat exchange assembly (10), and is characterized by further comprising a first heat exchange plate (101), a second heat exchange plate (102) and a disposable heat exchange tube (15), wherein the first heat exchange plate (101) and the second heat exchange plate (102) are detachably fixed, and a part of the disposable heat exchange tube (15) is arranged between the first heat exchange plate (101) and the second heat exchange plate (102) in a bending state;
One end of the disposable heat exchange tube (15) is fixedly provided with a sample storage cup (16), the interior of the sample storage cup (16) is communicated with the interior of the disposable heat exchange tube (15), a placement seat (1011) is fixedly arranged on the first heat exchange tube (101), the placement seat (1011) is used for placing the sample storage cup (16), the other end of the disposable heat exchange tube (15) vertically penetrates through the second heat exchange tube (102) downwards, and when the sample storage cup (16) is placed on the placement seat (1011), the liquid level of a sample dropped in the sample storage cup (16) is not more than the highest position of the bending position of the disposable heat exchange tube (15);
The sample storage cup (16) is fixedly provided with a fixing sheet (1601), a lifting control mechanism (12) is arranged in the case (1), and the lifting control mechanism (12) can lift the sample storage cup (16) through lifting the fixing sheet (1601) after contacting with the fixing sheet (1601).
2. The quantitative detection apparatus for fluorescence immunochromatography according to claim 1, in which the first conveying section comprises a conveyor belt (11), and the conveyor belt (11) is controlled by a driving motor.
3. The quantitative detection device for fluorescence immunochromatography according to claim 2, wherein the first conveying part comprises two identical conveying belts (11), two first mounting buckles (20) are fixed on the two conveying belts (11), two second mounting buckles (21) are fixed on each sample heat exchange assembly (10), the second mounting buckles (21) on the upper end of the same sample heat exchange assembly (10) are fixed with the first mounting buckles (20) on the conveying belts (11) on the upper end, the second mounting buckles (21) on the lower end are fixed with the first mounting buckles (20) on the conveying belts (11) on the lower end, the two first mounting buckles (20) fixed with the same sample heat exchange assembly (10) are located at the same vertical position, a central hole is formed in the center of each first mounting buckle (20), a first magnet (2001) is fixed in the central hole, a second mounting buckle (21) is provided with a center matched with the first magnet (2101) on the center, and a second magnet (2101) is arranged on the center of the center.
4. The quantitative detection device for fluorescence immunochromatography according to claim 1, wherein the second conveying part comprises a reagent card placing groove (5) and conveying belt (6) groups arranged on two sides of the reagent card placing groove (5), the conveying belt (6) groups are symmetrically arranged with the reagent card placing groove (5) as a center, each conveying belt (6) group comprises a conveying belt (6) and a plurality of belt wheels (7), and the plurality of belt wheels (7) are connected and driven by one conveying belt (6).
5. The quantitative detection device for fluorescence immunochromatography according to claim 4, wherein two connecting columns (501) are fixed at two ends of the reagent card placing groove (5), each end of the reagent card placing groove (5) is fixedly connected with the conveying belt (6) through the connecting column (501), an observation window (502) and an inserting opening (503) arranged at one side of the reagent card placing groove (5) are arranged on the reagent card placing groove (5), the observation window (502) is used for observing C and T lines of a reagent card, and the inserting opening (503) is used for inserting the reagent card into the reagent card placing groove (5).
6. The quantitative detection device for fluorescence immunochromatography according to claim 5, wherein a temperature control plate (13) is fixed at each of the drop sample position and the fluorescence detection position of the reagent card, when the conveyer belt (6) conveys the reagent card placing groove (5) to the temperature control plate (13), the temperature control plate (13) enables the reagent card placing groove (5) and the reagent card fixed on the reagent card placing groove (5) to maintain a horizontal state, a waste reagent card temporary storage area (8) is arranged at a lower position of a card insertion window in the case (1), waste reagent cards used for storing the used reagent cards are stored in the waste reagent card temporary storage area (8), and when the reagent card placing groove (5) on the conveyer belt (6) moves from a position close to the waste reagent card temporary storage area (8) to a card insertion window position, the card insertion opening (503) of the reagent card placing groove (5) faces downwards, and the reagent card placing groove (5) faces only the two sides of the fluorescent temporary storage area (503) in a vertical direction when the reagent card is in a direction from the card drop-detection position of the card insertion window to the reagent card drop-detection position.
7. The quantitative detection device for fluorescence immunochromatography according to claim 1, wherein a sample dropping port is formed in the top of the case (1), a cover plate (3) is arranged at the sample dropping port, one part of the cover plate (3) is hinged to the case (1), a sealing ring is fixed on the cover plate (3), a switch (14) is further fixed at the hinged position of the cover plate (3) and the case (1), the switch (14) is in a bouncing state when the cover plate (3) is opened, and the switch (14) is in a pressing state when the cover plate (3) is closed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202411709697.XA CN119199107B (en) | 2024-11-27 | 2024-11-27 | Quantitative detection device for fluorescence immunochromatography |
Applications Claiming Priority (1)
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| CA2873459A1 (en) * | 2012-05-15 | 2013-11-21 | Wellstat Diagnostics, Llc | Clinical diagnostic systems |
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| US10001501B2 (en) * | 2015-12-31 | 2018-06-19 | Universal Diagnostics, S. L. | Systems and methods for automated, customizable sample preparation for detection of metabolites and lipids |
| CN208568812U (en) * | 2018-08-14 | 2019-03-01 | 三诺生物传感股份有限公司 | A kind of Biochemical Analyzer |
| CN211086327U (en) * | 2019-08-09 | 2020-07-24 | 南京岚煜生物科技有限公司 | Light path detection assembly |
| CN110501514B (en) * | 2019-09-20 | 2023-12-22 | 四川朴澜医疗科技有限公司 | Automatic detector and automatic detection system |
| GB202015911D0 (en) * | 2020-10-07 | 2020-11-18 | Sigenex Inc | Systems and methods for processing a sample |
| CN113358884A (en) * | 2020-12-15 | 2021-09-07 | 南京诺唯赞医疗科技有限公司 | Fluorescence immunoassay system device and test method thereof |
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| CA2873459A1 (en) * | 2012-05-15 | 2013-11-21 | Wellstat Diagnostics, Llc | Clinical diagnostic systems |
| CN106592720A (en) * | 2015-10-20 | 2017-04-26 | 北京万生人和科技有限公司 | Water closet provided with urine analysis system |
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