CN119193729B - A method for preparing highly stable L-aspartic acid by synergistic catalysis - Google Patents
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Abstract
本发明涉及氨基酸制备领域，具体涉及一种协同催化高稳定L‑天冬氨酸的制备方法，通过结合组氨酸改性UiO‑67酶复合物和氨基酸改性ZIF‑8/Pebax‑1657混合基质膜技术，实现了高效的协同作用。组氨酸与UiO‑67的配位增强了酶的稳定性和活性，提高了L‑天冬氨酸的产率和纯度。同时，改性ZIF‑8/Pebax‑1657膜的高选择性和渗透性提升了分离效率，有效纯化L‑天冬氨酸，提高了L‑天冬氨酸的制备效率和产品质量，还减少了环境污染，实现了绿色生产和成本控制。
The present invention relates to the field of amino acid preparation, and in particular to a method for preparing highly stable L-aspartic acid by synergistic catalysis, which realizes efficient synergy by combining histidine-modified UiO-67 enzyme complex and amino acid-modified ZIF-8/Pebax-1657 mixed matrix membrane technology. The coordination of histidine and UiO-67 enhances the stability and activity of the enzyme, and improves the yield and purity of L-aspartic acid. At the same time, the high selectivity and permeability of the modified ZIF-8/Pebax-1657 membrane improve the separation efficiency, effectively purify L-aspartic acid, improve the preparation efficiency and product quality of L-aspartic acid, reduce environmental pollution, and realize green production and cost control.
Description
Technical Field
The invention relates to the technical field of amino acid preparation, in particular to a preparation method of synergistic catalysis high-stability L-aspartic acid.
Background
L-aspartic acid is used as a multifunctional amino acid, has wide application field and covers a plurality of industries such as food, medicine, chemical industry and the like. In the food industry, L-aspartic acid is used primarily as a nutritional supplement to enhance the nutritional value of food. In addition, L-aspartic acid is also used as a flavoring agent, added to various refreshing beverages, and used for enhancing the flavor of foods. The L-aspartic acid is also widely used in the medical field, and can be used as liver function promoter, ammonia antidote, fatigue recovery agent and the like. L-aspartic acid is also used for synthesizing various medical intermediates, is a main raw material for synthesizing L-alanine, and plays an important role in treating heart diseases and other diseases. In the chemical industry, L-aspartic acid is the main raw material for synthesizing polyaspartic acid which is a polymer material. In addition, the L-aspartic acid plays an important role in the agricultural field, and can promote the absorption of crops to nutrients, improve the germination rate of seeds and enhance the stress resistance of crops as a fertilizer synergist, thereby improving the agricultural production efficiency and reducing the cost. With the development of agricultural science and technology, the L-aspartic acid has wide application prospect in the agricultural field, and provides powerful support for realizing efficient, green and sustainable development of agricultural production.
L-aspartic acid, an important amino acid, has been widely used in various fields. At present, the production technology of L-aspartic acid is relatively mature, and the market demand is also growing. The domestic production scale is internationally at a leading level, but there are still some challenges and problems. First, in industrial production, L-aspartic acid is mainly produced in two steps, namely a chemical method and an enzyme catalysis method. The chemical method needs to be carried out at high temperature and high pressure, has high equipment requirements and is easy to cause environmental pollution. In addition, the traditional production process has the problems of high raw material purity requirement, multiple separation and purification steps, high energy consumption, large environmental pollution and the like, and the problems of cost and pollution greatly limit the market application and the value exertion of the L-aspartic acid. In the enzyme catalysis method, although the specificity is high, the cell consumption of catalyzing fumaric acid to L-aspartic acid by whole cells is large, and the maximum catalysis efficiency cannot be exerted. Second, there are problems of enzyme imbalance and catalytic-extraction cycling during biosynthesis of L-aspartic acid. There is a significant difference in the catalytic ability of the maleate isomerase and aspartase enzyme, resulting in poor synthesis efficiency.
In summary, the technical problems to be solved in the preparation process of L-aspartic acid include improvement of catalytic efficiency, reduction of environmental pollution, reduction of production cost, and the like, and the solution of these challenges is important to realize green and efficient production of L-aspartic acid.
Disclosure of Invention
The invention aims to provide a preparation method of synergistic catalysis high-stability L-aspartic acid, and the L-aspartic acid prepared by combining a histidine modified UiO-67 enzyme complex and an amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane technology is high in yield and purity. By using the histidine modified UiO-67 enzyme complex, the efficient conversion from fumaric acid to L-aspartic acid can be realized, the recycling of reaction liquid can be realized by a membrane concentration technology, the production cost is reduced, the utilization rate of raw materials is improved, the environmental pollution is reduced, the production cost is reduced, and the like.
In order to solve the technical problems, the invention provides a preparation method of synergistic catalysis high-stability L-aspartic acid.
In order to achieve the purpose, the invention provides the following technical scheme that the preparation method of the synergistic catalysis high-stability L-aspartic acid comprises the following steps:
Adding a histidine modified UiO-67 enzyme complex into a fumaric acid solution, and reacting for 6-20 hours at the temperature of 40-45 ℃ at 400r/min to obtain a first product, wherein the histidine modified UiO-67 enzyme complex comprises the following raw materials in a mass ratio of fumaric acid solution=1:70-150;
Filtering a product I, wherein a solid is a histidine modified UIO-67 enzyme complex, washing liquid with ultrapure water is used as a washing liquid I, mixing the washed histidine modified UIO-67 enzyme complex with an aspartase solution, adding an ammonium sulfate solution, stirring for 15min under ice bath conditions, adding glutaraldehyde under room temperature conditions, stirring for 15min, washing once with a tris (hydroxymethyl) aminomethane hydrochloride buffer solution, and drying to obtain a regenerated histidine modified UIO-67 enzyme complex, wherein the regenerated histidine modified UIO-67 enzyme complex can be continuously used in the step I, and the raw materials are used in a mass ratio of (1:0.01-0.1:0.8-1.2) of the washed histidine modified UIO-67 enzyme complex;
Heating the filtrate of the product I to 75-90 ℃, regulating the pH to be cooled and separated out crystals, leaching and drying to obtain L-aspartic acid and a washing liquid II, concentrating the mother solution after cooling and crystallizing, the washing liquid I and the washing liquid II through an amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane, regulating the pH of the concentrated mother solution, cooling and recrystallizing and separating out crystals, leaching and drying to obtain L-aspartic acid and a washing liquid III, mixing the washing liquid III and the mother solution after recrystallization, and adding fumaric acid for the step I.
Further, the preparation method of the histidine-modified UiO-67 enzyme complex comprises the following steps:
S1, dissolving zirconium tetrachloride in dimethylformamide, stirring for 15-30 min at the rotating speed of 600r/min to obtain a metal ion solution, wherein the mass ratio of the raw materials is calculated as that of zirconium tetrachloride, namely, dimethylformamide=1:35-63;
s2, dissolving 4, 4-biphenyldicarboxylic acid in dimethylformamide, stirring at the rotating speed of 600r/min for 15-30 min, adding histidine, and continuously stirring for 30min to obtain a ligand solution, wherein the mass ratio of the raw materials is (1:45-72:0.8-2.3) of 4, 4-biphenyldicarboxylic acid to dimethylformamide;
S3, pouring the ligand solution into a flask, placing the flask into a preheated oil bath pot with the temperature of 75-80 ℃ for rotating for 300r/min, dropwise adding the metal ion solution, after the dropwise adding, stirring for 2 hours at the rotating speed of 600r/min, heating to 95-110 ℃, continuously stirring for 5 hours, cooling the flask to room temperature in air, filtering, washing with ethanol for 3 times, and drying the product in a drying box at the temperature of 70 ℃ for 4-8 hours to obtain histidine modified UIO-67;
S4, mixing histidine modified UiO-67 with an aspartase solution, adding an ammonium sulfate solution, stirring for 1 hour under ice bath conditions, standing for 1 hour under room temperature conditions, adding glutaraldehyde, stirring for 30 minutes, washing three times by using a tris hydrochloride buffer solution, and drying to obtain the histidine modified UIO-67 enzyme complex, wherein the mass ratio of the histidine modified UiO-67 to the aspartase solution to the ammonium sulfate solution to glutaraldehyde=1:0.1-0.3:1.5-4 is 15-35 times that of the histidine modified UiO-67.
Further, the aspartase solution is prepared by dissolving aspartase in ultrapure water, wherein the mass ratio of the aspartase to the ultrapure water is 1:1-5.
Further, the fumaric acid solution is prepared by dissolving fumaric acid in ultrapure water, wherein the mass ratio of the fumaric acid to the ultrapure water is 1:5-15.
Further, the ammonium sulfate solution is prepared by dissolving ammonium sulfate in ultrapure water, wherein the concentration of the ammonium sulfate solution is 20% -50%.
Further, the preparation method of the amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane comprises the following steps:
Adding ZIF-8 nano particles into a methanol solution for dissolving threonine, wherein the mass ratio of the ZIF-8 nano particles to the threonine is that the methanol=1:0.75-1.55:50-100 is stirred for 2-4 hours at 40-80 ℃, and then filtering, washing and drying the mixture to obtain amino acid modified ZIF-8 nano particles;
Step 2, mixing absolute ethyl alcohol and ultrapure water, heating the mixture to 80-85 ℃ under stirring conditions in an oil bath, and then adding Pebax-1657 into the mixture, keeping the temperature of 80-85 ℃ and stirring for 4-5 hours to completely dissolve the mixture to obtain a Pebax-1657 solution, wherein the mass ratio of the Pebax-1657 to the absolute ethyl alcohol to the ultrapure water=1:5-20:10-25;
step 3, adding the amino acid modified ZIF-8 nano particles into the Pebax-1657 solution prepared in the step 2, and carrying out ultrasonic stirring and mixing to obtain a casting solution containing a filler, wherein the mass ratio of the amino acid modified ZIF-8 nano particles to the Pebax-1657 solution=1:12-23;
And 4, carrying out ultrasonic defoaming treatment on the casting solution obtained in the step3 for 10-30 min, standing for 4-10 min, pouring the casting solution onto a clean polytetrafluoroethylene flat plate, drying the casting solution in a constant-temperature oven at 60-80 ℃ for 18-24 h, and then placing the casting solution in a vacuum oven to be vacuumized for continuously removing the solvent for 18-24 h to obtain the amino acid modified ZIF-8/Pebax-1657 mixed matrix film.
Further, the preparation method of the tris hydrochloride buffer solution is that tris hydrochloride is dissolved in ultrapure water, and the concentration of the tris hydrochloride buffer solution is 5% -17%.
The preparation method of the synergistic catalytic high-stability L-aspartic acid has the beneficial effects that the preparation method of the synergistic catalytic high-stability L-aspartic acid combines the histidine modified UiO-67 enzyme complex and the amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane technology to prepare the L-aspartic acid, and has the advantage of synergistic effect. First, the addition of histidine-modified UiO-67 enzyme complex can improve the selectivity and efficiency of the reaction. Histidine is an amino acid which can form stable coordination with UiO-67 through the amino group, thereby enhancing the stability and activity of the enzyme. The modified enzyme complex can catalyze the reaction more effectively in the fumaric acid conversion process, and the yield and purity of the L-aspartic acid are improved. Meanwhile, the UiO-67 has larger pore diameter and specific surface area, and can accommodate more guest molecules, so that the reaction efficiency is improved. And secondly, the amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane is utilized for concentration, so that an efficient and environment-friendly separation process can be realized. The mixed matrix membrane combines the high selectivity of ZIF-8 with the high permeability of Pebax-1657, and can improve the self-diffusivity of molecules while maintaining the high selectivity, thereby improving the separation efficiency. In the preparation process of the L-aspartic acid, the membrane can effectively separate the L-aspartic acid from the reaction liquid, remove unwanted byproducts and impurities, improve the purity of the product, reduce the subsequent purification steps and reduce the production cost.
In conclusion, the technology combining the histidine modified UiO-67 enzyme complex and the amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane not only improves the preparation efficiency and the product quality of L-aspartic acid, but also reduces environmental pollution and realizes green production and cost control.
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FIG. 1 is a flow chart of a preparation method of synergistic catalytic high-stability L-aspartic acid provided by the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below in connection with the embodiments of the present invention. It will be apparent that the embodiments described are some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1, the embodiment of the invention provides a preparation method of synergistic catalysis high-stability L-aspartic acid.
The preparation method of the synergistic catalytic high-stability L-aspartic acid provided by the invention is specifically described by the following examples.
Example 1
Adding the histidine modified UiO-67 enzyme complex into fumaric acid solution (the mass ratio of fumaric acid to ultrapure water is 1:10), and reacting for 8 hours at the temperature of 45 ℃ at 400r/min to obtain a first product, wherein the histidine modified UiO-67 enzyme complex comprises the following raw materials in percentage by mass, namely fumaric acid solution=1:120;
Filtering a product I, wherein a solid is a histidine modified UIO-67 enzyme complex, washing liquid with ultrapure water is used as washing liquid I, mixing the washed histidine modified UIO-67 enzyme complex with an aspartase solution (the mass ratio of aspartase to ultrapure water is 1:3), adding a 50% ammonium sulfate solution, stirring for 15min under ice bath conditions, adding glutaraldehyde under room temperature conditions, stirring for 15min, washing with a 10% tris (hydroxymethyl) aminomethane hydrochloride buffer solution for one time, and drying to obtain a regenerated histidine modified UIO-67 enzyme complex, wherein the regenerated histidine modified UIO-67 enzyme complex can be continuously used in the step I, and the mass ratio of the raw materials is calculated according to the mass ratio.
Heating the filtrate of the product I to 85 ℃, regulating the pH to cool and separate out crystals, leaching and drying to obtain L-aspartic acid and a washing liquid II, concentrating the mother liquor after cooling and crystallizing, the washing liquid I and the washing liquid II through an amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane, regulating the pH of the concentrated mother liquor, cooling and recrystallizing to separate out crystals, leaching and drying to obtain L-aspartic acid and a washing liquid III, wherein the total yield of the L-aspartic acid reaches 89.9%, the purity reaches 98.6%, mixing the washing liquid III with the mother liquor after recrystallization, and adding the mother liquor into fumaric acid for the step I.
The preparation method of the histidine modified UiO-67 enzyme complex comprises the following steps:
S1, dissolving zirconium tetrachloride in dimethylformamide, stirring for 15min at the rotating speed of 600r/min to obtain a metal ion solution, wherein the mass ratio of the raw materials is calculated as that of zirconium tetrachloride, namely, dimethylformamide=1:40;
S2, dissolving 4, 4-biphenyldicarboxylic acid in dimethylformamide, stirring at 600r/min for 20min, adding histidine, and continuously stirring for 30min to obtain a ligand solution, wherein the mass ratio of the raw materials is calculated as 4, 4-biphenyldicarboxylic acid to dimethylformamide, and histidine=1:45:1.2;
S3, pouring the ligand solution into a flask, putting the flask into a preheated 80 ℃ oil bath kettle, rotating for 300r/min, dropwise adding the metal ion solution, stirring for 2 hours at 600r/min after the dropwise adding is finished, heating to 100 ℃, continuously stirring for 5 hours, cooling the flask to room temperature in air, filtering, washing with ethanol for 3 times, and drying the product in a drying oven at 70 ℃ for 6 hours to obtain histidine modified UiO-67;
S4, mixing histidine modified UiO-67 with an aspartase solution (the mass ratio of aspartase to ultrapure water is 1:2), adding a 40% ammonium sulfate solution, stirring for 1 hour under ice bath conditions, standing for 1 hour under room temperature conditions, adding glutaraldehyde, stirring for 30 minutes, washing with a 10% tris hydrochloride buffer solution for three times, and drying to obtain the histidine modified UIO-67 enzyme complex, wherein the mass ratio of the raw materials is calculated as the mass ratio, the histidine modified UiO-67: aspartase solution: ammonium sulfate solution: glutaraldehyde=1:0.2:2, and the mass of the tris hydrochloride buffer solution used for single washing is 20 times that of the histidine modified UiO-67.
The preparation method of the amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane comprises the following steps:
adding ZIF-8 nano particles into a methanol solution for dissolving threonine, wherein the mass ratio of the ZIF-8 nano particles to the threonine is that the methanol=1:1.25:50 is stirred for 24 hours at 75 ℃, filtering, washing and drying the mixture to obtain amino acid modified ZIF-8 nano particles;
Step 2, mixing absolute ethyl alcohol and ultrapure water, heating the mixture to 85 ℃ under the stirring condition in an oil bath, and then adding Pebax-1657 into the mixture, keeping the temperature of 80-85 ℃ and stirring the mixture for 4-5 hours to completely dissolve the mixture to obtain a Pebax-1657 solution, wherein the mass ratio of the Pebax-1657 to the absolute ethyl alcohol to the ultrapure water=1:8:15;
step 3, adding the amino acid modified ZIF-8 nano particles into the Pebax-1657 solution prepared in the step 2, and carrying out ultrasonic stirring and mixing to obtain a casting solution containing a filler, wherein the mass ratio of the amino acid modified ZIF-8 nano particles to the Pebax-1657 solution=1:18;
And 4, carrying out ultrasonic defoaming treatment on the casting solution obtained in the step 3 for 30min, standing for 5min, pouring the casting solution onto a clean polytetrafluoroethylene flat plate, putting the casting solution into a constant-temperature oven at 75 ℃ for drying for 8h, and then putting the casting solution into a vacuum oven for vacuumizing and continuously removing the solvent for 24h to obtain the amino acid modified ZIF-8/Pebax-1657 mixed matrix film.
Example 2:
Adding the histidine modified UiO-67 enzyme complex into fumaric acid solution (the mass ratio of fumaric acid to ultrapure water is 1:15), and reacting for 10 hours at the temperature of 45 ℃ at 400r/min to obtain a first product, wherein the histidine modified UiO-67 enzyme complex comprises the following raw materials in percentage by mass, namely fumaric acid solution=1:120;
Filtering a product I, wherein a solid is a histidine modified UIO-67 enzyme complex, washing liquid with ultrapure water is used as washing liquid I, mixing the washed histidine modified UIO-67 enzyme complex with an aspartase solution (the mass ratio of aspartase to ultrapure water is 1:4), adding a 40% ammonium sulfate solution, stirring the mixture for 15min under ice bath conditions, adding glutaraldehyde under room temperature conditions, stirring for 15min, washing the mixture once with a 12% tris (hydroxymethyl) aminomethane hydrochloride buffer solution, and drying the mixture to obtain a regenerated histidine modified UIO-67 enzyme complex, wherein the regenerated histidine modified UIO-67 enzyme complex can be continuously used in the step I, and the washed histidine modified UIO-67 enzyme complex comprises an aspartase solution comprising 40% ammonium sulfate solution comprising glutaraldehyde=1:0.1:1, wherein the mass of the washed product is 30 times that of histidine modified UiO-67;
Heating the filtrate of the product I to 75 ℃, regulating the pH to cool and separate out crystals, leaching and drying to obtain L-aspartic acid and a washing liquid II, concentrating the mother liquor after cooling and crystallizing, the washing liquid I and the washing liquid II through an amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane, regulating the pH of the concentrated mother liquor, cooling and recrystallizing to separate out crystals, leaching and drying to obtain L-aspartic acid and a washing liquid III, wherein the total yield of the L-aspartic acid reaches 92%, the purity reaches 98.7%, mixing the washing liquid III and the mother liquor after recrystallization, and adding fumaric acid for the step I.
The preparation method of the histidine modified UiO-67 enzyme complex is shown in example 1.
The preparation method of the amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane is shown in example 1.
Example 3:
Adding the histidine modified UiO-67 enzyme complex into fumaric acid solution (the mass ratio of fumaric acid to ultrapure water is 1:12), and reacting for 8 hours at the temperature of 45 ℃ at 400r/min to obtain a first product, wherein the histidine modified UiO-67 enzyme complex comprises the following raw materials in percentage by mass, namely fumaric acid solution=1:110;
Filtering a product I, wherein a solid is a histidine modified UIO-67 enzyme complex, washing liquid with ultrapure water is used as a washing liquid I, mixing the washed histidine modified UIO-67 enzyme complex with an aspartase solution (the mass ratio of aspartase to ultrapure water is 1:3.5), adding a 35% ammonium sulfate solution, stirring for 15min under ice bath conditions, adding glutaraldehyde, stirring for 15min at room temperature, washing with a 15% tris hydrochloride buffer solution for one time, and drying to obtain a regenerated histidine modified UIO-67 enzyme complex, wherein the regenerated histidine modified UIO-67 enzyme complex can be continuously used in the step I, and the mass ratio of the washed histidine modified UIO-67 enzyme complex to the aspartase solution to the 35% ammonium sulfate solution to glutaraldehyde=1:0.08:0.9, wherein the mass of the washed use 15% tris hydrochloride buffer solution is 30 times that of the histidine modified UiO-67;
Heating the filtrate of the product I to 85 ℃, regulating the pH to cool and separate out crystals, leaching and drying to obtain L-aspartic acid and a washing liquid II, concentrating the mother liquor after cooling and crystallizing, the washing liquid I and the washing liquid II through an amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane, regulating the pH of the concentrated mother liquor, cooling and recrystallizing to separate out crystals, leaching and drying to obtain L-aspartic acid and a washing liquid III, wherein the total yield of the L-aspartic acid reaches 89.5%, the purity reaches 98.5%, mixing the washing liquid III with the mother liquor after recrystallization, and adding the mother liquor into fumaric acid for the step I.
The preparation method of the histidine modified UiO-67 enzyme complex is shown in example 1.
The preparation method of the amino acid modified ZIF-8/Pebax-1657 mixed matrix membrane is shown in example 1.
Comparative example 1
Adding the UiO-67 enzyme complex into fumaric acid solution (the mass ratio of fumaric acid to ultrapure water is 1:12), reacting for 8 hours at 400r/min and 45 ℃ to obtain a first product, adjusting the pH value to be cooled to separate out crystals, leaching and drying to obtain L-aspartic acid, wherein the total yield of the L-aspartic acid reaches 62% and the purity reaches 93.7%, and the raw materials are calculated according to the mass ratio, wherein the UIO-67 enzyme complex comprises fumaric acid solution=1:110;
the preparation method of the UiO-67 enzyme complex comprises the following steps:
S1, dissolving zirconium tetrachloride in dimethylformamide, stirring for 15min at the rotating speed of 600r/min to obtain a metal ion solution, wherein the mass ratio of the raw materials is calculated as that of zirconium tetrachloride, namely, dimethylformamide=1:40;
S2, dissolving 4, 4-biphenyldicarboxylic acid in dimethylformamide, stirring at the rotating speed of 600r/min for 30min to obtain a ligand solution, wherein the mass ratio of the raw materials is 4, 4-biphenyldicarboxylic acid to dimethylformamide=1:45;
S3, pouring the ligand solution into a flask, putting the flask into a preheated 80 ℃ oil bath kettle, rotating for 300r/min, dropwise adding the metal ion solution, stirring for 2 hours at 600r/min after the dropwise adding is finished, heating to 100 ℃, continuously stirring for 5 hours, cooling the flask to room temperature in air, filtering, washing with ethanol for 3 times, and drying the product in a drying oven at 70 ℃ for 6 hours to obtain UIO-67;
s4, mixing UiO-67 with an aspartase solution (the mass ratio of aspartase to ultrapure water is 1:2), adding a 40% ammonium sulfate solution, stirring for 1 hour under ice bath conditions, standing for 1 hour under room temperature conditions, adding glutaraldehyde, stirring for 30 minutes, washing with a 10% tris hydrochloride buffer solution for three times, and drying to obtain the UIO-67 enzyme complex, wherein the mass ratio of the raw materials is (UiO-67) that of the aspartase solution to the ammonium sulfate solution to glutaraldehyde=1:0.2:2, and the mass of the tris hydrochloride buffer solution used for single washing is 20 times that of the histidine-modified UiO-67.
TABLE 1 summary of total yields and purities of L-aspartic acid according to examples 1-3 and comparative example 1
;
Summarizing, from Table 1, it is clear that the technology of the mixed matrix membrane of the histidine modified UiO-67 enzyme complex and the amino acid modified ZIF-8/Pebax-1657 can greatly improve the yield and the product purity of L-aspartic acid, and realize green production and cost control through recycling.
Finally, it should be noted that the above-mentioned embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same, and although the present invention has been described in detail with reference to examples, it should be understood by those skilled in the art that modifications and equivalents may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention, and all such modifications and equivalents are intended to be encompassed in the scope of the claims of the present invention.
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| CN109678809A (en) * | 2019-01-30 | 2019-04-26 | 山西大学 | The synthetic method and its application of the UIO-67 of tetrazole functionalization |
| CN111841536A (en) * | 2020-08-10 | 2020-10-30 | 昆山诺梵生物科技有限公司 | UiO-67 loaded Cu-based catalyst, and preparation method and application thereof |
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