CN119097577A - A method for preparing multi-dimensional co-assembled hydroponic oil and its application - Google Patents
A method for preparing multi-dimensional co-assembled hydroponic oil and its application Download PDFInfo
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- CN119097577A CN119097577A CN202411594873.XA CN202411594873A CN119097577A CN 119097577 A CN119097577 A CN 119097577A CN 202411594873 A CN202411594873 A CN 202411594873A CN 119097577 A CN119097577 A CN 119097577A
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- 239000012044 organic layer Substances 0.000 description 1
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- 150000003254 radicals Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
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- 238000005303 weighing Methods 0.000 description 1
Classifications
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/30—Characterized by the absence of a particular group of ingredients
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
本发明提供了一种制备多维共组装水培油的方法及其应用，属于油脂发酵技术领域。所述方法包括以下步骤：S1：取油不溶性物质，与大豆磷脂混合于无水乙醇中，于50‑70℃搅拌至溶液澄清，离心，取上清，挥干溶剂，得到复合物；S2：将壳聚糖、发酵油溶于浓度为0.5‑2 wt%的醋酸溶液中，混合均匀，静置，得到混合溶液；S3：将步骤S1得到的复合物溶于无水乙醇中，在匀速搅拌状态下缓慢滴加入步骤S2得到的混合溶液中，搅拌至其发出微蓝色乳光，得到共组装溶液；S4：将S3中共组装溶液添加至油脂中，得到多维共组装水培油。本发明所述方法可包裹油不溶性成分，使其溶于油脂中，结构稳定，还能提高有效成分的皮肤渗透和吸收性能，赋予油脂更多皮肤学功能活性。
The present invention provides a method for preparing multidimensional co-assembled hydroponic oil and its application, belonging to the field of oil fermentation technology. The method comprises the following steps: S1: taking oil-insoluble substances, mixing them with soybean lecithin in anhydrous ethanol, stirring at 50-70°C until the solution is clear, centrifuging, taking the supernatant, and evaporating the solvent to obtain a complex; S2: dissolving chitosan and fermented oil in an acetic acid solution with a concentration of 0.5-2 wt%, mixing evenly, standing to obtain a mixed solution; S3: dissolving the complex obtained in step S1 in anhydrous ethanol, slowly dropping it into the mixed solution obtained in step S2 under uniform stirring, stirring until it emits a slightly blue opalescent light, and obtaining a co-assembled solution; S4: adding the co-assembled solution in S3 to oil to obtain a multidimensional co-assembled hydroponic oil. The method of the present invention can encapsulate oil-insoluble components, dissolve them in oil, and have a stable structure. It can also improve the skin penetration and absorption performance of the effective ingredients, and give the oil more dermatological functional activity.
Description
Technical Field
The invention relates to the technical field of grease fermentation, in particular to a method for preparing multidimensional co-assembled hydroponic oil and application of the multidimensional co-assembled hydroponic oil in cosmetics and/or skin care products.
Background
The skin of the human body is mainly divided into epidermis, dermis and subcutaneous fat, wherein the epidermis is further divided into stratum corneum, transparent layer, granular layer, organic layer and basal layer. The outermost stratum corneum of the skin is composed of layers of keratin cells, and the cells of the stratum corneum are filled with ceramide, cholesterol, fatty acid and other structures.
Currently, in the oil and skin care product market, most of the products exist in the form of crude oil, such as sunflower seed oil, camellia seed oil, peony seed oil, jojoba oil, monkey tree oil and the like. However, crude oil lacks characteristic activity, and cannot play a certain active function while moisturizing skin, and most active substances cannot be stably present in grease. Therefore, the search for a suitable technology for stabilizing the oil-insoluble substances in the grease has great research value and market prospect for endowing the grease with characteristic functional activity.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides a method for preparing multidimensional co-assembled water culture oil and application thereof. The method can wrap the oil insoluble component, so that the oil insoluble component is dissolved in the grease, has stable structure, can improve the skin permeation and absorption performance of the effective component, and can endow the grease with more dermatological function activity.
The technical scheme of the invention is as follows:
a method for preparing multi-dimensional co-assembled hydroponic oil, comprising the steps of:
s1, mixing oil insoluble substances with soybean phospholipids in absolute ethyl alcohol, stirring at 50-70 ℃ until the solution is clear, centrifuging, taking supernatant, and volatilizing a solvent to obtain a compound;
S2, dissolving chitosan and fermentation oil in acetic acid solution with concentration of 0.5-2 wt%, uniformly mixing, and standing to obtain a mixed solution;
S3, dissolving the compound obtained in the step S1 in absolute ethyl alcohol, slowly dripping the compound into the mixed solution obtained in the step S2 under a uniform stirring state, and stirring until the compound emits light with bluish emulsion to obtain a co-assembly solution;
and S4, adding the co-assembly solution in the S3 into grease to obtain the multi-dimensional co-assembly water-cultured oil.
Preferably, in step S1, the oil-insoluble substance is at least one of glabridin, dihydroquercetin, ginsenoside, resveratrol, paeonol, naringin, hesperidin, tetrahydrocurcumin, quercetin, curcumin, anthocyanin, procyanidin, tea polyphenol, and arbutin, and the mass ratio of the oil-insoluble substance to soybean phospholipid is 1:8-10.
Further, the absolute ethyl alcohol in the step S1 is not limited.
Preferably, in step S2, the added amount of the chitosan is 1-1.5% of the mass of the acetic acid solution, and the added amount of the fermentation oil is 1-5% of the mass of the acetic acid solution.
Further, in step S2, the preparation method of the fermented oil includes the following steps:
m1, selecting a strain capable of producing a surfactant, and culturing the strain into seed liquid;
m2, inoculating the seed solution into a fermentation medium for pre-fermentation;
Adding grease for continuous fermentation;
and M4, after fermentation, adding a demulsifier to separate water and oil phases, and collecting an oil phase to obtain the fermentation oil.
Preferably, in the step M1, the strain capable of producing the surfactant is at least one of lactobacillus, bacillus, saccharomycete and corynebacterium glutamicum;
the culture medium formula adopted for culturing the strain into seed liquid is glucose 8-10 g/L, rice 15-20 g/L, and culturing at 28-30deg.C for 12-24 h after inoculation.
Preferably, in the step M2, the formula of the fermentation medium is 15-20 g/L glucose, 6-10 g/L yeast extract and 15-20 g/L rice, and the pre-fermentation is carried out by culturing 12-24 h at 28-30 ℃.
Preferably, in step M3 and step S4, the grease includes, but is not limited to, soybean oil, coconut oil, sunflower seed oil, jojoba seed oil, rose-hip oil, grape seed oil, almond oil, white pool seed oil, macadamia nut oil, olive fruit oil, stone chestnut seed oil, camellia seed oil, prinsepia utilis royle oil, sea buckthorn seed oil, peony seed oil, hazelnut oil, rice germ oil, wheat germ oil, glass chicory seed oil, safflower seed oil, plantain leaf blue thistle seed oil, bilberry seed oil, monkey seed oil, palm tree fruit oil, chile hazelnut oil, african wild mango oil, white lupin oil, olive shell oil, respectively.
Preferably, in the step M3, the volume ratio of the grease to the fermentation medium is 1:0.8-1.2, and the continuous fermentation time is 24-36h.
Further, the demulsifier in the step M4 is a combination of sodium chloride and hydrochloric acid, wherein the mass of the sodium chloride is 1.5-2.5% of that of the fermentation product, the mass of the hydrochloric acid is 0.1-0.5% of that of the fermentation product, and the concentration of the hydrochloric acid is 5-7M.
Preferably, in the step S3, the concentration of the compound after being dissolved in ethanol is 20-50 mg/mL, and the mass of the compound is 10-20% of the mass of the mixed solution.
Preferably, in the step S4, the adding amount of the co-assembly solution is 1-5% of the mass of the multi-dimensional co-assembly water-culture oil.
Further, the fermentation oil may also be refined using an integrated film system or silica gel.
The beneficial technical effects of the invention are as follows:
1. The lactobacillus selected by the invention can simultaneously produce a surfactant and a plurality of small molecular acid substances, wherein the surfactant has an emulsifying effect and can promote the generation of a co-assembly process, and the small molecular acid substances can promote the absorption of grease by a human body.
2. Compared with the traditional embedding or co-assembly technology, the non-natural oil and fat external emulsifying agent is not introduced in the co-assembly process, but the fermented oil prepared by strain fermentation is used for replacing the emulsifying agent function, so that the skin is more natural and has less irritation.
3. Compared with the traditional method for searching the grease with characteristic activity, the method for embedding the oil-insoluble substances by the co-assembly technology has the advantages that the obtained co-assembly water-cultured oil is stronger in effect, the characteristic activity of the grease can be artificially endowed, the use cost is lower, and the method is more convenient.
Drawings
FIG. 1 is a graph showing the results of the screening of the blood platelets of example 1;
fig. 2 is a schematic diagram of an oil drain red circle of example 2.
Detailed Description
The present invention will be described in detail below with reference to the drawings and examples. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
EXAMPLE 1 Primary screening of surfactant-producing Strain
The primary selection of the surfactant-producing strain is carried out by adopting a blood agar plate method, and the specific method is as follows:
(1) Respectively weighing 3g (3 mL) of soil samples (water samples) from Yan mountain Zhejiang, liyang Tianmu lake, nanjing Laoshan and Hebei Xingtai, supplementing 30 mL deionized water, and placing in 30 ℃ and 200 rpm for activation 30 min. And respectively transferring 1 mL activated soil suspension and activated water sample suspension to a20 mL enrichment culture medium, and placing the culture medium in a shaking table for shaking culture at 30 ℃ and 200 rpm for 2 days.
The formula of the enrichment medium (1L) is glucose 2g and yeast extract 0.2g,NH4NO30.2g,KH2PO40.3g,Na2HPO4·12H2O 0.5g,MgSO4·7H2O 0.05g.
(2) Enrichment culture solution of culture 2 d is taken, diluted 10 to 6-108 times in a gradient manner, 100 mu L of the enrichment culture solution is coated on a blood agar plate, and the enrichment culture solution is inversely cultured in a constant-temperature incubator at 30 ℃ for 24 h.
(3) The strain with larger hemolytic circle and clear edge (shown in figure 1) is selected, streaked and inoculated on a solid LB agar plate for standby, and the prepared glycerol tube is placed at-70 ℃ for preservation.
(4) And respectively carrying out 16S and ITS measurement on 7 strains with the best effect obtained by screening to obtain strains respectively including lactobacillus, bacillus subtilis, bacillus cereus, bacillus amyloliquefaciens, corynebacterium glutamicum, candida and pichia guilliermondii.
Example 2 surfactant producing Strain re-screening
The strain obtained by primary screening in the example 1 is further subjected to secondary screening by an oil discharge red ring method, and the specific method is as follows:
Clean glass petri dishes (diameter 9 cm) were placed on a piece of paper. 40 mL deionized water is added into a culture dish, and 200 mu L of oil red solution is added dropwise after the water surface is stable. After the oil red was completely and uniformly spread, 2.5. Mu.L of the supernatant of the fermentation broth was added dropwise to the center of the oil red surface. Recording the diameter of the oil drain ring after the transparent oil drain ring is stable in size. The surfactant activity generated by fermentation was judged by observing the diameter of the oil-extraction red circle (as shown in fig. 2), and the larger the diameter of the oil-extraction red circle, the stronger the surfactant activity generated by fermentation.
Experimental results show that the diameter of the lactobacillus oil discharge red ring is the largest, which indicates that the surfactant activity generated by fermentation is the strongest.
EXAMPLE 3 fat fermentation
The lactobacillus strains obtained by screening in the example 2 are used for preparing fermentation oil, and the specific process is as follows:
m1, inoculating lactobacillus obtained by screening in example 2 into seed culture medium, wherein the formula of the culture medium is glucose 10 g/L, rice 20 g/L, and culturing at 30deg.C for 12 h.
M2, inoculating the seed liquid cultured in the step M1 into a fermentation culture medium for pre-fermentation, wherein the formula of the culture medium is glucose 20 g/L, yeast extract 10 g/L, rice 20 g/L and 30 ℃ culture 12 h.
M3, adding sunflower seed oil with the volume 1 time of that of the fermentation medium, and continuing to ferment and culture for 24 h;
And M4, after fermentation is finished, adding a demulsifier to separate water and oil phases, centrifuging, and taking an upper oil phase to obtain the fermentation oil. The demulsifier is a composition of sodium chloride and hydrochloric acid, wherein the mass of the sodium chloride is 2% of that of the fermentation product, the mass of the hydrochloric acid is 0.1% of that of the fermentation product, and the concentration of the hydrochloric acid is 6M.
Example 4 preparation of Multi-dimensional Co-assembled hydroponic oil with whitening Properties
(1) Fully dissolving glabridin 1g and 10 g soybean phospholipid in absolute ethanol, stirring at 60 ℃ until the solution is clear and free of precipitate, centrifuging, collecting supernatant, and evaporating solvent to obtain glabridin compound.
(2) Taking chitosan 1 g and the fermentation oil 5g prepared in the example 3, dissolving the chitosan 1 g and the fermentation oil 5 in an acetic acid solution with the concentration of 1wt% in 100g, fully and uniformly stirring, standing to obtain a mixed solution, and taking 100g of the mixed solution for standby.
(3) Dissolving the glabridin compound prepared in the step (1) of 200 mg in 10 ml absolute ethyl alcohol, dropwise adding the glabridin compound into the mixed solution prepared in the step (2) of 100 g under uniform stirring, and continuously stirring until light blue opalescence appears, thus obtaining the co-assembly solution.
(4) And (3) adding the co-assembly solution 5g prepared in the step (3) into 95 g sunflower seed oil to obtain the multi-dimensional co-assembly water culture oil with whitening characteristics.
(5) The tyrosinase inhibition ability of the multidimensional co-assembled water culture oil was measured, and the sunflower seed crude oil was used as a control, and the specific results are shown in table 1.
TABLE 1 tyrosinase inhibition ability
From table 1, it can be seen that the water-cultured oil obtained by the co-assembly technology has much higher tyrosinase inhibition capability than the sunflower seed crude oil, and the invention endows the crude oil with whitening characteristic activity by the technology.
Example 5 preparation of Multi-dimensional Co-assembled hydroponic oil with antioxidant Activity
Taking dihydroquercetin 1 g, fully dissolving the dihydroquercetin 1 g and 8 g soybean phospholipid in absolute ethyl alcohol, stirring at 50 ℃ until the mixture is clear and free of precipitate, centrifuging, taking supernatant, and evaporating the solvent by rotary evaporation to obtain the dihydroquercetin compound.
Taking chitosan 1.5 g and the fermentation oil 1g prepared in the embodiment 3, dissolving the chitosan 1.5 g and the fermentation oil 1g in an acetic acid solution with the concentration of 100g being 1wt%, fully and uniformly stirring, standing to obtain a mixed solution, and taking 100g of the mixed solution for standby.
And (3) dissolving the dihydroquercetin compound prepared in the step (1) in 15 ml absolute ethyl alcohol, dropwise adding the dihydroquercetin compound into the mixed solution prepared in the step (2) in 100g under stirring, and continuously stirring until light blue opalescence appears, thus obtaining the co-assembly solution.
Adding the co-assembly solution 1 g prepared in the step (3) into 99 g sunflower seed oil to obtain the multi-dimensional co-assembly water-culture oil with antioxidant characteristics.
The DPPH clearance of the multidimensional co-assembled hydroponic oil was determined while sunflower seed crude oil was used as a control, and the specific results are shown in Table 2.
TABLE 2 DPPH scavenging Capacity
As can be seen from Table 2, the water-cultured oil obtained by the co-assembly technology has a DPPH free radical scavenging capability far higher than that of the sunflower seed crude oil, and the characteristic activity of scavenging free radicals of the crude oil is endowed by the technology.
Example 6 preparation of Multi-dimensional Co-assembled hydroponic oil with antibacterial Activity
Mixing ginsenoside 1 g with 9g soybean phospholipid and absolute ethanol, stirring at 50deg.C until the mixture is clear and has no precipitate, centrifuging, collecting supernatant, and evaporating solvent to obtain ginsenoside compound.
Taking chitosan 1.2 g and the fermentation oil 2.5 g prepared in the embodiment 3, dissolving in an acetic acid solution with the concentration of 100g being 1.5wt%, fully and uniformly stirring, standing to obtain a mixed solution, and taking 100g of the mixed solution for standby.
And (3) dissolving the ginsenoside compound prepared in the step (1) in the step of 360 mg in 12 ml absolute ethyl alcohol, dropwise adding the mixture into the mixed solution prepared in the step (2) of 100g under stirring, and continuously stirring until light blue opalescence appears, so as to obtain the co-assembly solution.
Adding 2.5g of the co-assembly solution prepared in the step (3) into 97.5 g sunflower seed oil to obtain the multi-dimensional co-assembly water-based oil with antibacterial activity characteristics.
The size of the bacteriostasis ring of the multidimensional co-assembled water culture oil is measured, and meanwhile, sunflower seed crude oil is used as a control, and the diameter of the bacteriostasis ring is expressed. The specific results are shown in Table 3.
Table 3 bacteriostatic ability test
As can be seen from Table 3, the water-cultured oil obtained by the co-assembly technology has much higher antibacterial capacity than the sunflower seed crude oil. The invention imparts antibacterial characteristic activity to crude oil by the technology.
Although the embodiments of the present invention have been disclosed in the foregoing description and drawings, it is not limited to the details of the embodiments and examples, but is to be applied to all the fields of application of the present invention, it will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the spirit and scope of the invention as defined by the appended claims and their equivalents.
Claims (10)
1. A method for preparing multidimensional co-assembled water culture oil, which is characterized by comprising the following steps:
s1, mixing oil insoluble substances with soybean phospholipids in absolute ethyl alcohol, stirring at 50-70 ℃ until the solution is clear, centrifuging, taking supernatant, and volatilizing a solvent to obtain a compound;
S2, dissolving chitosan and fermentation oil in acetic acid solution with concentration of 0.5-2 wt%, uniformly mixing, and standing to obtain a mixed solution;
S3, dissolving the compound obtained in the step S1 in absolute ethyl alcohol, slowly dripping the compound into the mixed solution obtained in the step S2 under a uniform stirring state, and stirring until the compound emits light with bluish emulsion to obtain a co-assembly solution;
and S4, adding the co-assembly solution in the S3 into grease to obtain the multi-dimensional co-assembly water-cultured oil.
2. The method according to claim 1, wherein in the step S1, the oil-insoluble substance is at least one of glabridin, dihydroquercetin, ginsenoside, resveratrol, paeonol, naringin, hesperidin, tetrahydrocurcumin, quercetin, curcumin, anthocyanin, procyanidin, tea polyphenol, and arbutin, and the mass ratio of the oil-insoluble substance to soybean phospholipid is 1:8-10.
3. The method according to claim 1, wherein in the step S2, the chitosan is added in an amount of 1-1.5% by mass of the acetic acid solution, and the fermentation oil is added in an amount of 1-5% by mass of the acetic acid solution.
4. The method according to claim 1, wherein in step S2, the method for preparing the fermented oil comprises the steps of:
m1, selecting a strain capable of producing a surfactant, and culturing the strain into seed liquid;
m2, inoculating the seed solution into a fermentation medium for pre-fermentation;
Adding grease for continuous fermentation;
and M4, after fermentation, adding a demulsifier to separate water and oil phases, and collecting an oil phase to obtain the fermentation oil.
5. The method according to claim 4, wherein in the step M1, the strain capable of producing the surfactant is at least one of lactobacillus, bacillus, yeast and corynebacterium glutamicum;
the culture medium formula adopted for culturing the strain into seed liquid is glucose 8-10 g/L, rice 15-20 g/L, and culturing at 28-30deg.C for 12-24 h after inoculation.
6. The method according to claim 4, wherein in the step M2, the fermentation medium is prepared from 15-20 g/L glucose, 6-10 g/L yeast extract and 15-20 g/L rice, and the pre-fermentation is performed by culturing 12-24 h at 28-30 ℃.
7. The method of claim 1 or 4, wherein in steps M3 and S4, the grease comprises, but is not limited to, soybean oil, coconut oil, sunflower seed oil, jojoba seed oil, rose-hip oil, grape seed oil, almond oil, white pool seed oil, macadamia nut oil, olive fruit oil, stone chestnut seed oil, camellia seed oil, prinsepia utilis royle oil, sea buckthorn seed oil, peony seed oil, hazelnut oil, rice germ oil, wheat germ oil, glass chicory seed oil, safflower seed oil, plantain seed oil, bilberry seed oil, monkey seed oil, palm tree fruit oil, zhili hazelnut oil, african mango oil, white lupin oil, olive shell oil, respectively.
8. The method according to claim 4, wherein in the step M3, the ratio of the volume of the fat to the volume of the fermentation medium in the step M2 is 1:0.8-1.2, and the fermentation is continued for 24-36 hours.
9. The method according to claim 1, wherein in step S3, the concentration of the complex after dissolution in ethanol is 20-50 mg/mL, and the mass of the complex is 10-20% of the mass of the mixed solution.
10. The method according to claim 1, wherein in step S4, the amount of the co-assembly solution added is 1-5% of the mass of the multi-dimensional co-assembly hydroponic oil.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN119606800A (en) * | 2025-02-11 | 2025-03-14 | 杭州佳嘉乐生物技术有限公司 | Preparation method of 11-seed fermentation oil and fermentation liquor with multiple effects |
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| CN117586828A (en) * | 2023-12-01 | 2024-02-23 | 杭州佳嘉乐生物技术有限公司 | Preparation method of high-permeability antioxidant crude oil |
| CN118086355A (en) * | 2024-01-19 | 2024-05-28 | 稀物集(广州)生物科技有限公司 | A strain enriched with active ingredients of pine mushrooms and a method for preparing pine mushroom fermented oil |
| CN118750423A (en) * | 2024-08-13 | 2024-10-11 | 贵州极海果蔬饮品工程技术有限公司 | A roxburghii extract and its application in cosmetics or skin care products |
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| CN103110107A (en) * | 2013-01-20 | 2013-05-22 | 浙江大学 | Method for wrapping tea polyphenol by using protein gel |
| CN115317410A (en) * | 2022-08-29 | 2022-11-11 | 广州那比昂生物科技有限公司 | Fermented oil capable of relieving and moisturizing and enhancing cell viability as well as preparation method and application of fermented oil |
| CN117586828A (en) * | 2023-12-01 | 2024-02-23 | 杭州佳嘉乐生物技术有限公司 | Preparation method of high-permeability antioxidant crude oil |
| CN118086355A (en) * | 2024-01-19 | 2024-05-28 | 稀物集(广州)生物科技有限公司 | A strain enriched with active ingredients of pine mushrooms and a method for preparing pine mushroom fermented oil |
| CN118750423A (en) * | 2024-08-13 | 2024-10-11 | 贵州极海果蔬饮品工程技术有限公司 | A roxburghii extract and its application in cosmetics or skin care products |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN119606800A (en) * | 2025-02-11 | 2025-03-14 | 杭州佳嘉乐生物技术有限公司 | Preparation method of 11-seed fermentation oil and fermentation liquor with multiple effects |
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