CN119016126A - Microfluidic chip and water quality detection system - Google Patents
Microfluidic chip and water quality detection system Download PDFInfo
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- CN119016126A CN119016126A CN202411517855.1A CN202411517855A CN119016126A CN 119016126 A CN119016126 A CN 119016126A CN 202411517855 A CN202411517855 A CN 202411517855A CN 119016126 A CN119016126 A CN 119016126A
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- 238000001514 detection method Methods 0.000 title claims abstract description 132
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 61
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 123
- 239000007788 liquid Substances 0.000 claims abstract description 88
- 230000002572 peristaltic effect Effects 0.000 claims description 56
- 230000029087 digestion Effects 0.000 claims description 25
- 238000012546 transfer Methods 0.000 claims description 21
- 239000012086 standard solution Substances 0.000 claims description 18
- 239000002699 waste material Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 238000007599 discharging Methods 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000010453 quartz Substances 0.000 claims description 2
- 238000012372 quality testing Methods 0.000 claims 1
- 230000010354 integration Effects 0.000 abstract description 10
- 238000004458 analytical method Methods 0.000 abstract description 3
- 238000002835 absorbance Methods 0.000 description 22
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 20
- 238000000034 method Methods 0.000 description 13
- 239000000126 substance Substances 0.000 description 12
- 239000012530 fluid Substances 0.000 description 11
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- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 10
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 10
- 229910052757 nitrogen Inorganic materials 0.000 description 10
- 229910052698 phosphorus Inorganic materials 0.000 description 10
- 239000011574 phosphorus Substances 0.000 description 10
- 229910002651 NO3 Inorganic materials 0.000 description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 8
- 238000002372 labelling Methods 0.000 description 8
- 239000001301 oxygen Substances 0.000 description 8
- 229910052760 oxygen Inorganic materials 0.000 description 8
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 8
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 7
- 229910019142 PO4 Inorganic materials 0.000 description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 6
- 239000000987 azo dye Substances 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 6
- 239000010452 phosphate Substances 0.000 description 6
- 230000002378 acidificating effect Effects 0.000 description 5
- 238000004891 communication Methods 0.000 description 5
- -1 nitrate ions Chemical class 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 4
- 229940010552 ammonium molybdate Drugs 0.000 description 4
- 235000018660 ammonium molybdate Nutrition 0.000 description 4
- 239000011609 ammonium molybdate Substances 0.000 description 4
- 125000001477 organic nitrogen group Chemical group 0.000 description 4
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 4
- 238000005086 pumping Methods 0.000 description 4
- 229960004889 salicylic acid Drugs 0.000 description 4
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
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- LECSHJWIACEDPZ-UHFFFAOYSA-N ethane-1,2-diamine naphthalene hydrochloride Chemical compound C(CN)N.C1=CC=CC2=CC=CC=C12.Cl LECSHJWIACEDPZ-UHFFFAOYSA-N 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
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- DHRLEVQXOMLTIM-UHFFFAOYSA-N phosphoric acid;trioxomolybdenum Chemical compound O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.OP(O)(O)=O DHRLEVQXOMLTIM-UHFFFAOYSA-N 0.000 description 2
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 2
- KQROHCSYOGBQGJ-UHFFFAOYSA-N 5-Hydroxytryptophol Chemical compound C1=C(O)C=C2C(CCO)=CNC2=C1 KQROHCSYOGBQGJ-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- GEHMBYLTCISYNY-UHFFFAOYSA-N Ammonium sulfamate Chemical compound [NH4+].NS([O-])(=O)=O GEHMBYLTCISYNY-UHFFFAOYSA-N 0.000 description 1
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 238000011481 absorbance measurement Methods 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
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- 125000003277 amino group Chemical group 0.000 description 1
- 238000004164 analytical calibration Methods 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
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- 229910052805 deuterium Inorganic materials 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
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- 239000003344 environmental pollutant Substances 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
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- 239000010865 sewage Substances 0.000 description 1
- 239000005368 silicate glass Substances 0.000 description 1
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- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
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- 238000012795 verification Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Plasma & Fusion (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
本申请涉及水质检测分析技术领域，公开了一种微流控芯片以及水质检测系统，微流控芯片包括芯体，芯体内形成有样本流道用于注入样本，标液流道适于注入标液或试剂，汇流流道与样本流道和标液流道相连，试剂流道为至少一条，且试剂流道与汇流流道连通，试剂流道适于注入试剂；汇流流道分支出连通至计量通道的第一支路，以及连通至检测通道的第二支路。前述芯体具有较高的集成化程度，将样本、标液以及试剂可选择的组合后通过汇流流道汇流至计量通道或者检测通道，并且可以通过试剂流道或者标液流道通入不同的试剂，以实现不同类型的水样参数检测，从而在一个芯体上实现样本的多种参数检测。
The present application relates to the technical field of water quality detection and analysis, and discloses a microfluidic chip and a water quality detection system. The microfluidic chip includes a core body, a sample flow channel is formed in the core body for injecting samples, a standard liquid flow channel is suitable for injecting standard liquid or reagents, a confluence flow channel is connected to the sample flow channel and the standard liquid flow channel, there is at least one reagent flow channel, and the reagent flow channel is connected to the confluence flow channel, and the reagent flow channel is suitable for injecting reagents; the confluence flow channel branches out a first branch connected to a metering channel, and a second branch connected to a detection channel. The aforementioned core body has a high degree of integration, and the sample, standard liquid and reagent are optionally combined and then converged to the metering channel or the detection channel through the confluence flow channel, and different reagents can be introduced through the reagent flow channel or the standard liquid flow channel to realize different types of water sample parameter detection, thereby realizing multiple parameter detection of samples on one core body.
Description
Technical Field
The application relates to the technical field of water quality detection and analysis, in particular to a micro-fluidic chip and a water quality detection system.
Background
When water quality detection is carried out, various detection needs to be carried out on a sample to be detected to determine various parameters of the sample, but the integration degree of the existing water quality detection system or device is low, and when various parameters are detected, corresponding results can be obtained only by carrying out different detection on the sample in different environments or devices, so that the defects of low water quality detection efficiency, large sample consumption, high cost and the like are caused.
Disclosure of Invention
The present application aims to solve at least one of the technical problems existing in the prior art. Therefore, an object of the present application is to provide a microfluidic chip for improving the integration degree of the microfluidic chip, thereby improving the water quality detection efficiency and reducing the cost.
The application further provides a water quality detection system comprising the microfluidic chip.
In a first aspect, a microfluidic chip according to an embodiment of the present application includes a core, in which a sample flow channel, a standard liquid flow channel, a confluence flow channel, a reagent flow channel, a metering channel, and a detection channel are formed, the sample flow channel is used for injecting a sample, the standard liquid flow channel is at least one and is arranged in parallel with the sample flow channel, the standard liquid flow channel is suitable for injecting a standard liquid or a reagent, the confluence flow channel is connected with the sample flow channel and the standard liquid flow channel, the reagent flow channel is at least one, and the reagent flow channel is communicated with the confluence flow channel, and the reagent flow channel is suitable for injecting a reagent; the converging flow channel branches to a first branch communicated with the metering channel and a second branch communicated with the detection channel, the reagent flow channel, the sample flow channel and the standard liquid flow channel are all communicated to the first branch, the other end of the metering channel is connected with the first peristaltic pump, the other end of the detection channel is communicated with the atmosphere, the first branch is further communicated with the second peristaltic pump and the digestion module in sequence, and the first branch is communicated with the second branch in a selectable mode.
According to the microfluidic chip provided by the embodiment of the application, the integration degree of the core body is improved, the sample, the standard solution and the reagent are optionally combined and then are converged to the metering channel or the detection channel through the converging flow channel, and different reagents can be introduced through the reagent flow channel or the standard solution flow channel, so that the detection of different types of water sample parameters is realized, and the detection of various parameters of the water body sample is realized on one core body.
According to a further embodiment of the application, a microfluidic chip comprises: the first plate body and the second plate body are bonded to define a sample flow channel, a standard liquid flow channel, a confluence flow channel, a reagent flow channel, a metering channel and a detection channel, wherein the volumes of the metering channel and the detection channel are 50 ul-250 ul, the length is 10 mm-50 mm, the width is 0.5 mm-3 mm, and one end communicated with the confluence flow channel is provided with a chamfer.
According to a further embodiment of the present application, the reagent flow channel is communicated with the metering channel and the detecting channel at the center position of the metering channel and the detecting channel, and the through-flow diameters of the sample flow channel, the standard liquid flow channel, the confluence flow channel and the reagent flow channel are 0.5 mm-1 mm.
According to a further embodiment of the application, at least part of the converging flow channels are projected in a U-shape in the thickness direction of the core, and the closed section of the U-shape is higher than the open section of the U-shape.
In accordance with a further embodiment of the present application, a reagent flow channel comprises: a first reagent flow channel, a second reagent flow channel, and a third reagent flow channel.
In some embodiments, the detection channel has a first detection region and a second detection region, the metering channel has a first metering region and a second metering region, and the first detection region, the second detection region, the first metering region, and the second metering region are each provided with at least one set of light sources and receivers.
According to a further embodiment of the application, the core further comprises: the auxiliary flow channel, the first switching flow channel and the second switching flow channel; the sample flow channel and the target flow channel are both communicated to the auxiliary flow channel, and the auxiliary flow channel is communicated to the converging flow channel through the first switching flow channel and the second switching flow channel.
Further, the core is constructed as a piece of quartz material.
In a second aspect, a water quality detection system according to an embodiment of the present application includes: the microfluidic chip, the first peristaltic pump, the second peristaltic pump, and the digestion module of any of the foregoing embodiments, the first peristaltic pump being connected to the metering channel; the second peristaltic pump is connected with the second branch.
In accordance with a further embodiment of the present application, the water quality detection system further comprises: the three-way valve is connected with the first peristaltic pump, the second port is connected with the digestion module, and the third port is used for discharging waste liquid.
Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.
Drawings
The foregoing and/or additional aspects and advantages of the application will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic view of a first plate structure of a core according to an embodiment of the present application;
FIG. 2 is an exploded block diagram of a core according to an embodiment of the present application;
FIG. 3 is an exploded view of another angle of a core according to an embodiment of the present application;
FIG. 4 is a top view of a core according to an embodiment of the application;
FIG. 5 is a schematic cross-sectional view corresponding to line A-A of FIG. 4 according to an embodiment of the present application;
FIG. 6 is a simplified schematic diagram of an internal flow path of a core according to an embodiment of the application;
FIG. 7 is a simplified flow chart of ammonia nitrogen detection according to an embodiment of the present application.
Reference numerals:
100-core;
110-a first plate body and 120-a second plate body;
210-sample flow channel;
220-a standard liquid flow channel, 221-a first standard liquid flow channel, 222-a second standard liquid flow channel;
230-confluence flow passage, 231-first branch, 2311-first section branch, 2312-second section branch, 2313-second pump port, 232-second branch, 233-first confluence flow passage, 234-second confluence flow passage, 235-third confluence flow passage, 236-closed section, 237-liquid inlet section, 238-liquid outlet section;
240-reagent flow channel, 241-first reagent flow channel, 242-second reagent flow channel, 243-third reagent flow channel;
250-metering channel, 251-first metering zone, 252-second metering zone, 253-first pump port, 254-first chamfer;
260-detection channel, 261-first detection zone, 262-second detection zone, 263-exhaust port;
270-auxiliary flow channel, 271-first transfer flow channel, 272-second transfer flow channel;
310-a first peristaltic pump, 320-a second peristaltic pump;
400-digestion module;
510-first valve body, 520-second valve body, 530-third valve body, 540-fourth valve body, 550-fifth valve body, 560-sixth valve body, 570-seventh valve body, 580-eighth valve body, 590-ninth valve body;
600-three-way valve, 610-first port, 620-second port, 630-third port.
Detailed Description
Embodiments of the present application are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the application.
In describing embodiments of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present application and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.
In the description of embodiments of the application, a "first feature" or "second feature" may include one or more of such features.
In the description of the embodiments of the present application, "plurality" means two or more.
In describing embodiments of the present application, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, or may include both the first and second features not being in direct contact but being in contact with another feature therebetween.
In the description of embodiments of the application, a first feature "above," "over" and "over" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature.
First, some technical terms related to the present application will be explained:
Total nitrogen is the total amount of inorganic and organic nitrogen in various forms in water. Inorganic nitrogen including nitrate ions (NO 3 -), nitrite ions (NO 2 -) and amino groups (NH 4 +) and organic nitrogen including proteins, amino acids and organic amines, calculated as milligrams of nitrogen per liter of water. Are often used to represent the degree to which a body of water is contaminated with nutrients.
Total phosphorus refers to the total amount of phosphorus in all forms in water, including inorganic phosphorus and organic phosphorus. Total phosphorus is an important index in water environments. The main sources of the total phosphorus include domestic sewage, chemical fertilizers, phosphate cleaning agents used in organophosphorus pesticides and detergents, and the like. Excess phosphorus in the water body is one of the main reasons for causing pollution and foreign odor of the water body, eutrophication of lakes and red tide in gulf.
The compound nitrogen in the form of free ammonia (NH 3) and ammonium ions (NH 4 +) is called ammonia nitrogen. Ammonia nitrogen is a nutrient in a water body, can cause water eutrophication, is a main oxygen consumption pollutant in the water body, and is toxic to fish and certain aquatic organisms.
Chemical oxygen demand COD (Chemical Oxygen Demand) is a measure of the amount of reducing species in a water sample that need to be oxidized by chemical means. Oxygen equivalent of substances (typically organic substances) that can be oxidized by strong oxidants in wastewater, wastewater treatment plant effluent and contaminated water.
And (3) marking liquid: also called standard solution, plays a critical role in the water quality analysis process, wherein the main roles comprise instrument calibration, method verification, quality control, result comparison, instrument performance monitoring, method standardization and the like. A microfluidic chip and a water quality detection system according to an embodiment of the present application are described below with reference to fig. 1 to 6.
The microfluidic chip according to the embodiment of the application comprises a core body 100 arranged in the microfluidic chip, wherein the core body 100 is a core component of the microfluidic chip for realizing online water quality monitoring, and is used for realizing water quality detection, and a sample flow channel 210, a standard liquid flow channel 220, a confluence flow channel 230, a reagent flow channel 240, a metering channel 250 and a detection channel 260 are formed in the core body 100.
The following specifically describes with reference to fig. 1.
Wherein the sample flow channel 210 is used for injecting a sample.
Specifically, the sample is a water sample to be detected, and the sample is injected through the flow channel to detect various parameters.
For example, total nitrogen detection is performed to obtain the total amount of inorganic and organic nitrogen in various forms in water; for another example, a total phosphorus test is performed to obtain the total amount of phosphorus in all forms in the water; for another example, ammonia nitrogen detection is performed to obtain the total amount of compound nitrogen in the water body, which exists mainly in the form of free ammonia (NH 3) and ammonium ions (NH 4 +); also for example, chemical oxygen demand COD detection is performed to obtain the oxygen equivalent of a substance (typically an organic substance) in the sample that can be oxidized by a strong oxidizing agent.
The labeling fluid channel 220 is suitable for injecting labeling fluid or reagent, and the labeling fluid channel 220 is one or more, and is arranged in parallel with the sample channel 210. When the number of the liquid channels 220 is one, the liquid channels 220 are connected in parallel with the sample channels 210, and when the number of the liquid channels 220 is plural, the liquid channels 220 are connected in parallel with each other and the sample channels 210.
The sample flow channel 210 and the target flow channel 220 are connected in parallel to the converging flow channel 230.
In some cases, the label fluid flow channel 220 is used for the incoming label fluid. In other cases, the label flow channel 220 may also be used to introduce reagents to effect the detection of a water quality parameter of the sample. Therefore, the label fluid channel 220 and the sample fluid channel 210 are arranged in parallel to realize the function of simultaneously inputting the sample and the label fluid, or simultaneously inputting the sample and the reagent, and of course, the label fluid channel 220 can be multiplexed to sequentially inject the label fluid and the reagent, or sequentially inject the reagent and the label fluid.
The reagent flow channels 240 are one or more, the plurality of reagent flow channels 240 are connected in parallel with each other, each reagent flow channel 240 is collected to the confluent flow channel 230, and the reagent flow channels 240 are in communication with the confluent flow channel 230, the reagent flow channels 240 being adapted for injection of a reagent.
Specifically, the sample flow channel 210 and the target liquid flow channel 220 input the sample and the target liquid, or the sample and the reagent, to the confluence flow channel 230; the reagent flow channel 240 inputs a reagent into the confluence flow channel 230. As can be seen, the confluence flow channel 230 enables confluence of the sample and the labeling solution, or the sample and the reagent, or the sample, the labeling solution, and the reagent, and delivers the confluent liquid to the metering channel 250 or the detection channel 260 after confluence.
The confluence flow channel 230 branches into a first branch 231 communicated with the metering channel 250 and a second branch 232 communicated with the detection channel 260, the reagent flow channel 240, the sample flow channel 210 and the standard solution flow channel 220 are all communicated with the first branch 231, the other end of the metering channel 250 is connected with the first peristaltic pump 310, the other end of the detection channel 260 is communicated with the atmosphere, wherein the first branch 231 is also communicated with the second peristaltic pump 320 and the digestion module 400 in sequence, and the first branch 231 is communicated with the second branch 232 in a selectable manner.
Specifically, the first branch 231 may branch out of the bypass pipe to be sequentially communicated with the second peristaltic pump 320 and the digestion module 400, and the confluence flow channel 230 may implement different internal circulation modes according to the communication condition between the first branch 231 and the second branch 232, for example, a valve body is disposed between the first branch 231 and the second branch 232 to implement the communication between the first branch 231 and the second branch 232.
In an exemplary case, the first branch 231 and the second branch 232 are not communicated, and the reagent flow channel 240, the sample flow channel 210, and the standard flow channel 220 merge into the metering channel 250 through the first branch 231.
In another exemplary case, the first branch 231 communicates with the second branch 232, so that the reagent flow channel 240, the sample flow channel 210 and the label flow channel 220 communicate with the second branch 232 through the first branch 231, and the confluence in the detection channel 260 is realized through the second branch 232.
According to the microfluidic chip of the embodiment of the application, through improving the integration degree of the core 100, the sample, the standard solution and the reagent are optionally combined and then converged to the metering channel 250 or the detection channel 260 through the converging flow channel 230, and different reagents can be introduced through the reagent flow channel 240 or the standard solution flow channel 220, so that different types of water sample parameter detection can be realized, and thus, multiple parameter detection of the water sample can be realized on one core 100.
In some embodiments, it is understood with reference to the illustrations of FIGS. 2-4.
The core body 100 is integrally plate-shaped and comprises a first plate body 110 and a second plate body 120, the first plate body 110 and the second plate body 120 are bonded, grooves are formed in the planes of the first plate body 110 and the second plate body 120 for bonding, and after the first plate body 110 and the second plate body 120 are bonded, the grooves of the first plate body 110 and the second plate body 120 are spliced to define a sample flow channel 210, a standard liquid flow channel 220, a confluence flow channel 230, a reagent flow channel 240, a metering channel 250 and a detection channel 260. The first plate 110 and the second plate 120 may be made of quartz glass, PDMS, silicate glass, acryl, or the like.
In some examples, the first plate 110 and the second plate 120 are 3-5mm thick, the measurement channel 250 and the detection channel 260 have a volume of 50 ul-250 ul, a length of 10 mm-50 mm, and a width of 0.5 mm-3 mm. The cavity is a long and narrow cuboid cavity, the length is 10-50mm, and the width and the height are 0.5-3mm.
For example, the thickness of the first plate 110 and the second plate 120 is 3mm, when the first plate 110 and the second plate 120 are bonded, a core 100 with a thickness of 6mm is formed, for example, the thickness of the first plate 110 and the second plate 120 is 5mm, when the first plate 110 and the second plate 120 are bonded, a core 100 with a thickness of 10mm is formed, for example, the thickness of the first plate 110 and the second plate 120 is 4mm, and when the first plate 110 and the second plate 120 are bonded, a core 100 with a thickness of 8mm is formed.
Illustratively, the metering channel 250 and the detection channel 260 have a volume of 50ul, a length of 10mm, and a width of 0.5mm, for example, to input less bodily fluid into the metering channel 250 and the detection channel 260 to complete the detection. For example, the volume of the metering channel 250 and the detecting channel 260 is 250ul, the length is 50mm, and the width is 3mm, so that more body fluid can be input into the metering channel 250 and the detecting channel 260 to complete detection, and for example, the volume of the metering channel 250 and the detecting channel 260 is 60ul, the length is 15mm, and the width is 1mm.
It can be understood that the metering channel 250 is used for metering, when metering, the local area needs to be irradiated by an external light source to achieve the purpose of metering, and the detection channel 260 in the same way is used for obtaining absorbance based on the irradiation of the external light source to the local area, so that the metering channel 250 and the detection channel 260 are configured into long and narrow cuboid cavities, the irradiation area of the light source to the metering channel 250 or the detection channel 260 can be reduced, and the accuracy of detection or metering is further improved.
In some examples, the first branch 231 is connected to the center of the metering channel 250, and the second branch 232 is connected to the center of the detection channel 260, and the flow diameters of the sample channel 210, the flow channel 220, the confluence channel 230, and the reagent channel 240 are 0.5 mm-1 mm.
Specifically, when the flow diameters of the sample flow channel 210, the target flow channel 220, the confluence flow channel 230 and the reagent flow channel 240 are 0.5mm, the liquid in each flow channel is less. For example, when the flow diameters of the sample flow channel 210, the target flow channel 220, the confluence flow channel 230, and the reagent flow channel 240 are 1mm, more liquid is contained in each flow channel. Alternatively, the flow diameters of the sample flow channel 210, the target flow channel 220, the confluence flow channel 230, and the reagent flow channel 240 may be 0.8mm.
It will be appreciated that by arranging the first branch 231 at the center of the metering channel 250 and the second branch 232 at the center of the detection channel 260, liquid is collected from the centers of the metering channel 250 and the detection channel 260, so that the microfluidic chip can be prevented from hanging on the inner wall of the metering channel 250 or the detection channel 260 due to bubbles generated by the flow of the liquid during operation.
The core 100 constructed based on the corresponding specific parameters has the characteristics of miniaturization and integration, and the projected area of the core 100 in the thickness direction in the example is reduced to a rectangular structure of 72mm by 62mm, so that all functions of sampling, liquid distribution, metering and detection are integrated on the miniaturized core 100.
Further, the end of the metering channel 250 communicating with the converging channel 230 and the end of the detecting channel 260 communicating with the converging channel 230 are provided with chamfers, specifically, a first chamfer 254 is provided in the metering channel 250 and a second chamfer (not shown) is provided in the detecting channel 260
Referring to fig. 2-5, the first chamfer 254 is illustrated as an example of the metering channel 250.
The first branch 231 in the confluence flow path 230 communicates with the metering channel 250, and herein, an end of the metering channel 250 communicating with the first branch 231 is defined as a first end. As shown in fig. 2-3, the metering channel 250 formed by combining the first plate body 110 and the second plate body 120 is integrally formed as a rectangular hollow structure, and is communicated with the first branch 231 at the center of the first end, and the first end is configured with a plurality of first chamfer angles 254 which are arranged around the center of the first end in a surrounding manner, for example, two first chamfer angles 254 which are arranged oppositely as shown in fig. 5.
The second chamfer in the detection channel 260 is substantially identical to the first chamfer 254 in the metering channel 250 and will not be described in detail herein.
It will be appreciated that the chamfer structure can direct the flow of liquid in the metering channel 250 or the detection channel 260 in a chamfer direction to avoid bubbles or liquid forming a residue in the metering channel 250 or the detection channel 260.
To better illustrate the flow channels within the core 100 of an embodiment of the present application, a detailed description is provided below in conjunction with fig. 1 and 6.
The core 100 is provided with a plurality of valve bodies, and the valve bodies can be controlled to open or close by controlling the valve bodies so as to control the liquid circulation in the core 100. The valve bodies include a first valve body 510, a second valve body 520, a third valve body 530, a fourth valve body 540, a fifth valve body 550, a sixth valve body 560, a seventh valve body 570, an eighth valve body 580, and a ninth valve body 590. The ninth valve body 590 is configured to connect the first branch 231 and the second branch 232.
It is further noted that the metering channel 250 is connected to the first pump port 253 at one end and the first branch 231 at the other end. The first pump port 253 is used for being externally connected to the first peristaltic pump 310, the second pump port 2313 is arranged at the middle section of the first branch 231, and the second pump port 2313 is used for being externally connected to the second peristaltic pump 320. The first branch 231 is divided into a first segment branch 2311 and a second segment branch 2312 by a second pump port 2313, the first segment branch 2311 is used for being connected to the metering channel 250, the middle segment of the second segment branch 2312 is communicated with the confluence flow channel 230, and the outlet end is connected to the ninth valve body 590. In addition, the detection channel 260 is connected to the second branch 232 at one end and the exhaust port 263 at the other end (i.e., the other end of the detection channel is connected to the atmosphere).
Referring to fig. 1 and 6, the reagent flow path 240 includes a first reagent flow path 241, a second reagent flow path 242, and a third reagent flow path 243, the three reagent flow paths 240 are respectively used for connecting different reagents, and a valve body for controlling the connection and disconnection is provided between each reagent flow path 240 and the confluence flow path 230.
Specifically, a sixth valve body 560 is provided between the first reagent flow path 241 and the confluence flow path 230, and two interfaces of the sixth valve body 560 are connected to the first reagent flow path 241 and the confluence flow path 230, respectively, and the opening and closing between the first reagent flow path 241 and the confluence flow path 230 are controlled by the sixth valve body 560. A seventh valve body 570 is provided between the second reagent flow path 242 and the confluence flow path 230, and two ports of the seventh valve body 570 are connected to the second reagent flow path 242 and the confluence flow path 230, respectively, and the opening and closing between the second reagent flow path 242 and the confluence flow path 230 are controlled by the seventh valve body 570. An eighth valve body 580 is provided between the third reagent flow channel 243 and the confluence flow channel 230, and two ports of the eighth valve body 580 are connected to the third reagent flow channel 243 and the confluence flow channel 230, respectively, and the opening and closing between the third reagent flow channel 243 and the confluence flow channel 230 are controlled by the sixth valve body 560.
It will be appreciated that the configuration of three reagent flow channels 240 allows for gating between different reagent flow channels 240 and the manifold flow channel 230, allowing different reagents to pass into either the metering channel 250 or the sensing channel 260 depending on the circumstances to allow for different parameter sensing. The integration of three reagent flow channels 240 within the same core 100 and the gating achieved by the valve body helps to increase the degree of integration of the core 100, thereby integrating multiple parameter sensing functions on one core 100.
Further, the core 100 further defines a secondary flow channel 270, a first transfer flow channel 271 and a second transfer flow channel 272 connected to the secondary flow channel 270, and the secondary flow channel 270 is also connected to the sample flow channel 210, the first standard solution flow channel 221 and the second standard solution flow channel 222.
For better illustration of the first transfer flow channel 271 and the second transfer flow channel 272, the flow channels converging to the second segment branch 2312 in the converging flow channel 230 shown in fig. 1 are defined as: a first merging flow path 233, a second merging flow path 234, and a third merging flow path 235. One end of the first transfer flow channel 271 is communicated with the auxiliary flow channel 270, and the other end is directly communicated with the second converging flow channel 234; the second switching flow channel 272 has one end connected to the auxiliary flow channel 270 and the other end directly connected to the third confluence flow channel 235, and the second reagent flow channel 242 is connected to the second confluence flow channel 234 and the third reagent flow channel 243 is connected to the third confluence flow channel 235.
The standard solution runner 220 is connected to the auxiliary runner 270 through the first valve body 510, the first standard solution runner 221 is connected to the auxiliary runner 270 through the second valve body 520, the second standard solution runner 222 is connected to the auxiliary runner 270 through the third valve body 530, the auxiliary runner 270 is connected to the first transfer runner 271 through the fourth valve body 540, the auxiliary runner 270 is transferred to the second transfer runner 272 through the fifth valve body 550, the first reagent runner 241 is connected to the first confluence runner 233 through the sixth valve body 560, the second reagent runner 242 is connected to the second confluence runner 234 through the seventh valve body 570, and the third reagent runner 243 is connected to the third confluence runner 235 through the eighth valve body 580. Each valve body controls the connection and stop between the two flow channels through the opening and closing of the valve body.
It can be understood that the flow channels 220 and 210 are respectively connected to the corresponding flow channels 230 through the auxiliary flow channel 270, the first transfer flow channel 271 and the second transfer flow channel 272, so as to realize multiplexing of the flow channels 230 (the same flow channel 230 is used for passing different liquids), thereby realizing the function of inputting multiple reagents to the core 100 for detection, and completing detection of different parameters. Therefore, multiplexing the confluence flow channel 230 can not only improve the integration degree of the core 100, but also realize the function of simultaneously inputting multiple types of reagents and further completing the detection of different parameters.
In combination with the foregoing, it can be seen that:
The sample enters the core 100 from the sample flow channel 210, enters the auxiliary flow channel 270 through the first valve body 510, further enters the first transfer flow channel 271 through the fourth valve body 540, the liquid further enters the second confluence flow channel 234 from the first transfer flow channel 271, the ninth valve body 590 is kept closed, and the liquid is converged into the metering channel 250 from the first branch 231; after metering is completed, the ninth valve body 590 is opened so that liquid passes through the ninth valve body 590 into the sensing passage 260.
The labeling solution enters the core 100 from the first labeling solution channel 221 or the second labeling solution channel 222, further enters the first transfer channel 271 or the second transfer channel 272 from the fourth valve body 540 or the fifth valve body 550 from the auxiliary channel 270, and the rest is substantially identical to the sample flow mode, and the process is not repeated.
The reagent enters the core 100 from the first reagent flow channel 241, the second reagent flow channel 242, or the third reagent flow channel 243, and the reagent flow channel 240 is directly connected to the confluence flow channel 230, so that the first reagent flow channel 241, the second reagent flow channel 242, and the third reagent flow channel 243 are respectively converged into the first branch 231 from the first confluence flow channel 233, the second confluence flow channel 234, and the third confluence flow channel 235, and the subsequent processes are substantially identical to the two processes, and are not repeated herein.
In some examples, the converging flow passage 230 is U-shaped in projection in the thickness direction of the core 100, and the closed section 236 of the U-shape is higher than the open section of the U-shape.
As will be further understood with reference to fig. 1, the first merging flow path 233, the second merging flow path 234, and the third merging flow path 235 are all U-shaped and are sequentially disposed from outside to inside, and the first merging flow path 233 will be described in detail.
The U-shaped structure of the first converging flow passage 233 projected in the thickness direction of the core 100 includes two sections of linear flow passages and one section of arc-shaped flow passage, the openings of the open sections of the two sections of linear flow passages are respectively connected to the second section branch 2312 and the first reagent flow passage 241, and the arc-shaped section is the closed section 236. For better illustration, the straight line segment on the side leading to the first reagent flow channel 241 is defined as the liquid inlet segment 237, and the straight line segment on the side leading to the second segment branch 2312 is defined as the liquid outlet segment 238.
It will be appreciated that when testing of different parameters is performed, various liquids flow from the liquid inlet segment 237 into the converging channel 230, through the closed segment 236 (i.e. the arc-shaped portion), and then to the liquid outlet segment 238, after the liquid flow is completed, the liquid outlet segment 238 has higher hydraulic pressure compared to the liquid inlet segment 237 directly communicating with the detecting channel 260 and the metering channel 250 because the detecting channel 260 and the metering channel 250 are in a filled state. The closed segment 236 (i.e., the arc segment) is configured such that the projection in the thickness direction is higher than the two straight segments (i.e., the liquid inlet segment 237 and the liquid outlet segment 238), so that the backflow of the liquid due to the pressure difference can be blocked.
In order to detect different parameters of the liquid, the microfluidic chip further includes a plurality of light sources (not shown) and a plurality of receivers (not shown), the light sources and the receivers are disposed in pairs on two sides of the thickness of the core 100, at least one group of light sources and the receivers are disposed corresponding to the metering channel 250 and the detection channel 260, and the light emission wavelengths of the plurality of light sources are different.
The light source of the micro-fluidic chip can be a light source which can emit specific wavelength, such as a xenon lamp, a deuterium lamp and the like, is connected with a micro-battery, and can also be a photodiode, a spectrometer and the like, and the micro-battery is irradiated by the light source and is received by a receiver after passing through liquid so as to realize a corresponding detection function.
In the process of introducing the liquid into the metering channel 250 by the first branch 231 during metering or in the process of introducing the liquid into the detection channel 260 by the second branch 232 during detection, the corresponding channels are gradually filled with the liquid, so that metering or detection can be performed under different filling degrees according to the filling degree of the liquid into the channels.
For example, the detection channel 260 has a first detection region 261 and a second detection region 262, the metering channel 250 has a first metering region 251 and a second metering region 252, and the first detection region 261, the second detection region 262, the first metering region 251, and the second metering region 252 are each provided with at least one set of light sources and receivers.
The metering region on the side of the metering channel 250 away from the first branch 231 is a first metering region 251, and the side of the metering channel 250 close to the first branch 231 is a second metering region 252. When the liquid fills and fills to half the volume of the metering channel 250, the liquid volume in the second metering region 252 is 50ul, and the liquid in the second metering region 252 is metered based on the light sources and the receivers on both sides of the first metering region 251. The volume of liquid in the metering channel 250 is 100ul when the light sources and receivers on both sides of the first metering region 251 meter while the metering channel 250 is full of liquid.
Similarly, two detection areas, namely a first detection area 261 and a second detection area 262, are respectively arranged in the detection channel 260, each detection area is respectively provided with a light source and a receiver, the two are respectively positioned at two sides of the microfluidic chip and are oppositely arranged, and the light source luminous points corresponding to the first detection area 261 are positioned below the liquid level of 60ul according to the filling degree of the liquid in the detection channel 260, and the light source luminous points corresponding to the second detection area 262 are positioned below the liquid level of 150ul, so that the description is omitted.
Several detection scenarios are illustrated next.
For example, when detecting ammonia nitrogen in a sample to be detected, the first detection region 261 uses 230 nm light to measure the absorbance of the water sample, and the second detection region 262 uses 275 nm light to measure the absorbance of the water sample.
For another example, first detection zone 261 employs 700nm illumination to measure absorbance of a water sample when total nitrogen is applied to a sample to be detected.
For another example, when Chemical Oxygen Demand (COD) is detected on a sample to be detected, the first detection zone 261 employs 254 nm illumination to measure the absorbance of the water sample.
The water quality detection system according to the embodiment of the application comprises: the microfluidic chip described in any of the previous embodiments, and a first peristaltic pump 310, a second peristaltic pump 320, and a digestion module 400, the first peristaltic pump 310 being connected to the metering channel 250, the second peristaltic pump 320 being connected to the second branch 232.
Specifically, a ninth valve body 590 is disposed between the second peristaltic pump 320 and the second branch 232, and the ninth valve body 590 is used to control the on/off between the second peristaltic pump 320 and the second branch 232, and of course, as shown in fig. 1, the second peristaltic pump 320 may be connected to the middle section of the first branch 231 through the second pump opening 2313, and then be communicated with the second branch 232 through the first branch 231, and a ninth valve body 590 is disposed between the first branch 231 and the second branch 232 to control the on/off between the second peristaltic pump 320 and the second branch 232.
Further, the system comprises a three-way valve 600, wherein a first port 610 of the three-way valve 600 is connected to the second peristaltic pump 320, a second port 620 is connected to the digestion module 400, and a third port 630 is used for discharging waste liquid. Wherein, the digestion module 400 is used for performing a heating digestion process.
Illustratively, referring to fig. 6, the second peristaltic pump 320 is coupled to the first leg 231, with the first leg 231 and the second leg 232 coupled by a ninth valve 590 and with the ninth valve 590 controlling the communication between the first leg 231 and the second leg 232. The second peristaltic pump 320 is thus in communication with the ninth valve body 590, the second branch 232 through the first branch 231. The second peristaltic pump 320 is connected to the first port 610 of the three-way valve 600, and discharges waste liquid to the third port 630 or conveys liquid to the digestion module 400 by the driving action of the second peristaltic pump 320.
It will be appreciated that the delivery of liquid to the digestion module 400 or to the detection channel 260 or to the discharge of waste liquid is accomplished by a three-way valve 600 and a second peristaltic pump 320. Thereby realizing various functions on the structure of the core 100 and further improving the integration level of the core 100.
Next, with continued understanding with reference to fig. 1 and fig. 6, the operation of the detection system according to the embodiment of the present application will be described by taking the case of ammonia nitrogen detection as an example.
Specifically, the ammonia nitrogen detection adopts a salicylic acid spectrophotometry, nitrate in a sample is required to be reduced into nitrite by a reducing agent under an acidic condition, and then the nitrite reacts with salicylic acid under the acidic condition to generate red azo dye. The color shade of the azo dye is proportional to the concentration of nitrite, the absorbance of which is measured by a spectrophotometer at a specific wavelength, and the maximum absorption wavelength of the dye is usually between 630 and 700 nanometers. The concentration of ammonia nitrogen in the water sample can be determined by measuring the absorbance at this wavelength by lambert beer's law.
As will be understood with reference to fig. 7, the detection includes the following steps:
s1, pumping a sample to be detected, which is externally connected with the sample flow channel 210, into the core body 100 through the first peristaltic pump 310.
Under the action of the suction force of the first peristaltic pump 310, the sample to be detected enters the auxiliary flow channel 270 through the first valve body 510, the auxiliary flow channel 270 enters the first transfer flow channel 271 through the fifth valve body 550, and then the liquid is converged to the first branch 231 after entering the third converging flow channel 235 from the first transfer flow channel 271, and finally flows into the metering channel 250 through the first branch 231 for metering.
S2, pushing the sample to be detected in the metering channel into the digestion module 400 through the first peristaltic pump 310.
After S1 is completed, the driving direction of the first peristaltic pump 310 is reversed such that the sample to be detected in the metering channel 250 is inputted into the first branch 231, the first port 610 and the second port 620 of the three-way valve 600 are kept in an open state, the third port 630 is closed, and then inputted into the digestion module 400 through the second peristaltic pump 320 and the first port 610 and the second port 620 of the three-way valve 600.
S3, pumping the reagent externally connected with the first reagent flow channel 241 into the core body 100 through the first peristaltic pump 310.
As shown in fig. 1 and 6, the driving direction of the first peristaltic pump 310 is reversed again, so that the pumping action of the liquid is achieved, the third reagent flow channel 243 is connected to the third confluence flow channel 235 through the eighth valve body 580, so that the eighth valve body 580 can be switched to an open state to achieve multiplexing of the third confluence flow channel 235, and the first reagent is input into the first branch 231 through the third confluence flow channel 235 and pumped to the metering channel 250 for metering. Specifically, the first reagent is a solution of sulfuric acid and amine sulfamate.
S4, pushing the first reagent in the metering channel into the digestion module 400 through the first peristaltic pump 310.
The process in this step is substantially identical to the process in S2, the sample to be detected is replaced with the first reagent, and is heated to perform the reduction reaction after being mixed with the sample to be detected in the digestion module 400, and is cooled after the reaction is completed.
S5, pumping the second reagent externally connected with the first standard liquid channel 221 into the core body 100 through the first peristaltic pump 310.
The second reagent is introduced into the auxiliary flow channel 270 from the first standard liquid flow channel 221, the subsequent flow process of the second reagent is basically consistent with that of the S1, and the sample to be detected is replaced by the second reagent.
S6, pushing the first reagent in the metering channel into the digestion module 400 through the first peristaltic pump 310.
This step is substantially consistent with S2, the sample to be tested is replaced with a second reagent and input to the digestion module 400 to be mixed with other liquids. Specifically, the second reagent is a salicylic acid solution and a naphthalene ethylenediamine hydrochloride solution, and an azo dye solution is formed after the salicylic acid solution and the naphthalene ethylenediamine hydrochloride solution are mixed with other solutions in the digestion module 400 for a period of time.
S7, injecting azo dye solution into the detection channel 260 through the second peristaltic pump 320.
The second peristaltic pump 320 pumps the azo dye solution in the digestion module 400 into the first leg 231 while closing the first peristaltic pump 310 and opening the detection channel 260 for the passage of external air so that the azo dye solution cannot enter the metering channel 250 but only into the detection channel 260 for absorbance testing. And finally, preparing a standard curve by using a nitrate nitrogen standard solution with known concentration, and determining the concentration of ammonia nitrogen in the water sample by comparing absorbance.
Next, other exemplary cases will be briefly described in connection with the description of the previous example.
For example, total phosphorus detection uses ammonium molybdate spectrophotometry, where ammonium molybdate reacts with phosphate under acidic conditions to form yellow phosphomolybdic acid complex whose concentration is proportional to the concentration of phosphate in the water sample. Under acidic conditions, the phosphate reacts with ammonium molybdate to form a yellow phosphomolybdic acid complex with a maximum absorption wavelength of typically about 420 nanometers. By measuring the absorbance at this wavelength, the phosphate content of the water sample can be quantitatively analyzed by lambert beer's law.
During detection, a sample to be detected is pumped by the first peristaltic pump 310, is input into the metering channel 250 for metering through the first valve body 510 and the fifth valve body 550, and then the metered water sample is injected into the detection channel 260 through the ninth valve body 590 by the first peristaltic pump 310. The sulfuric acid solution is then pumped by a first peristaltic pump 310 and metered through the metering channel 250 and injected into the detection channel 260.
Then, the ascorbic acid solution and the ammonium molybdate solution are injected into the detection channel 260 in this order in the same manner, and then left to stand for a certain period of time after being sufficiently mixed to complete the color reaction. And finally measuring the absorbance of the developed solution under the illumination of the wavelength of about 700 nanometers. Finally, a standard curve is made by using phosphate standard solution with known concentration, and the concentration of phosphate in the water sample is determined by comparing absorbance.
For another example, total nitrogen detection uses ultraviolet spectrophotometry, under acidic conditions, potassium persulfate is used as an oxidant to oxidize organic nitrogen, ammonia nitrogen and nitrite nitrogen in the water sample to nitrate. Nitrate has stronger absorption in the ultraviolet light region of about 220 nanometers, and the absorbance is proportional to the concentration of nitrate. By measuring the absorbance at this wavelength, the total nitrogen content in the water sample can be quantitatively analyzed by lambert beer's law.
During detection, a sample to be detected is pumped by the first peristaltic pump 310 and enters the metering channel 250 for metering through the first valve body 510 and the fifth valve body 550. The metered water sample is then injected into digestion module 400 by first peristaltic pump 310. Then the potassium persulfate solution and the sulfuric acid solution are respectively pumped by the first peristaltic pump 310, are metered by the metering channel 250 and then are injected into the digestion module 400, and are heated for a certain time to be digested and oxidized after being fully mixed.
After oxidation and cooling to room temperature, the mixed liquid was injected into the detection channel 260 by the second peristaltic pump 320, and absorbance of the mixed liquid was measured under light having a wavelength of 230 nm. Oxidative digestion and absorbance measurements were performed as described above using a series of standard solutions of nitrate of known concentration. And drawing a standard curve by taking the nitrate concentration as an abscissa and the absorbance as an ordinate. And according to the absorbance of the sample, the corresponding nitrate concentration is obtained on a standard curve, namely the total nitrogen concentration.
For another example, in Chemical Oxygen Demand (COD) detection, the pretreated water sample is pumped into the detection channel 260 by the first peristaltic pump 310 and the absorbance of the water sample is measured under light having a wavelength of 254 nm. Using a standard solution of known chemical oxygen demand detection result (hereinafter abbreviated as COD value), UV254 absorbance of the standard solution was measured and a calibration curve was established.
Specifically, UV254 refers to ultraviolet absorbance at 254 nm wavelength, which is an indicator of the organic content of a large amount of water, especially the organic content containing conjugated double bonds or aromatic rings. There is a certain correlation between UV254 and COD, so the COD value can be estimated rapidly by measurement of UV 254.
The calibration curve relates COD value to UV254 absorbance. And calculating the COD value according to the UV254 absorbance of the water sample through a calibration curve. In the process, the first standard flow channel 221 and the second standard flow channel 222 are respectively used for introducing the standard solution to obtain a corresponding standard result.
It can be understood that the detection system of the embodiment of the application realizes the detection of various parameters of water quality by using the highly integrated micro-fluidic chip, thereby improving the water quality detection efficiency, reducing the dosage of reagents and standard liquid and reducing the use cost.
In the description of the present specification, reference to the terms "one embodiment," "some embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the present application have been shown and described, it will be understood by those of ordinary skill in the art that: many changes, modifications, substitutions and variations may be made to the embodiments without departing from the spirit and principles of the application, the scope of which is defined by the claims and their equivalents.
Claims (10)
1. A microfluidic chip, comprising: a core body (100), wherein a sample flow channel (210), a standard liquid flow channel (220), a confluence flow channel (230), a reagent flow channel (240), a metering channel (250) and a detection channel (260) are formed in the core body (100);
The sample flow channel (210) is used for injecting a sample, the standard liquid flow channel (220) is at least one and is arranged in parallel with the sample flow channel (210), the standard liquid flow channel (220) is suitable for injecting standard liquid or reagent, the confluence flow channel (230) is connected with the sample flow channel (210) and the standard liquid flow channel (220), the reagent flow channel (240) is at least one, the reagent flow channel (240) is communicated with the confluence flow channel (230), and the reagent flow channel (240) is suitable for injecting reagent;
The confluence flow channel (230) branches into a first branch (231) communicated with the metering channel (250) and a second branch (232) communicated with the detection channel (260), the reagent flow channel (240), the sample flow channel (210) and the standard liquid flow channel (220) are all communicated with the first branch (231), the other end of the metering channel (250) is connected with a first peristaltic pump (310), the other end of the detection channel (260) is communicated with the atmosphere, wherein
The first branch (231) is also communicated with the second peristaltic pump (320) and the digestion module (400) in sequence, and the first branch (231) is communicated with the second branch (232) in a selectable mode.
2. The microfluidic chip according to claim 1, wherein the microfluidic chip comprises: the device comprises a first plate body (110) and a second plate body (120), wherein the first plate body (110) is bonded with the second plate body (120) to define a sample flow channel (210), a standard liquid flow channel (220), a confluence flow channel (230), a reagent flow channel (240), a metering channel (250) and a detection channel (260), the volumes of the metering channel (250) and the detection channel (260) are 50 ul-250 ul, the lengths are 10 mm-50 mm, the widths are 0.5 mm-3 mm, and one end communicated with the confluence flow channel (230) is provided with a chamfer.
3. The microfluidic chip according to claim 1, wherein the reagent flow channel (240) is communicated with the metering channel (250) and the detection channel (260) at a central position of the metering channel (250) and the detection channel (260), and the diameters of the sample flow channel (210), the standard solution flow channel (220), the confluence flow channel (230) and the reagent flow channel (240) are 0.5 mm-1 mm.
4. The microfluidic chip according to claim 1, wherein at least part of the confluence channels (230) are U-shaped in projection in the thickness direction of the core (100), and the closed section (236) of the U-shape is higher than the open section of the U-shape.
5. The microfluidic chip according to claim 1, wherein the reagent flow channel (240) comprises: a first reagent flow channel (241), a second reagent flow channel (242), and a third reagent flow channel (243).
6. The microfluidic chip according to claim 5, wherein the detection channel (260) has a first detection region (261) and a second detection region (262), the metering channel (250) has a first metering region (251) and a second metering region (252), and the first detection region (261), the second detection region (262), the first metering region (251) and the second metering region (252) are each provided with at least one set of light sources and receivers.
7. The microfluidic chip according to any one of claims 1-6, further comprising: an auxiliary flow passage (270), a first transfer flow passage (271) and a second transfer flow passage (272);
The sample flow channel (210) and the standard liquid flow channel (220) are both communicated with the auxiliary flow channel (270), and the auxiliary flow channel (270) is communicated with the confluence flow channel (230) through the first transfer flow channel (271) and the second transfer flow channel (272).
8. The microfluidic chip according to claim 7, wherein the core (100) is configured as a piece of quartz material.
9. A water quality testing system, comprising:
The microfluidic chip of any one of claims 1-8;
-a first peristaltic pump (310), said first peristaltic pump (310) being connected to said metering channel (250);
A second peristaltic pump (320) and a digestion module (400), the second peristaltic pump (320) being connected to the second branch (232).
10. The water quality detection system of claim 9, further comprising: and the three-way valve (600), a first port (610) of the three-way valve (600) is connected with the second peristaltic pump (320), a second port (620) is connected with the digestion module (400), and a third port (630) is used for discharging waste liquid.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110927373A (en) * | 2019-11-26 | 2020-03-27 | 北京化工大学 | Full-automatic multi-index joint detection micro-fluidic chip and device |
| CN114471762A (en) * | 2022-02-22 | 2022-05-13 | 西北大学 | Design and implementation method of micro-fluidic chip capable of controlling ultralow flow velocity |
| CN117025376A (en) * | 2023-07-06 | 2023-11-10 | 浙江工业大学 | Rapid sterile detection method based on liquid drop micro-fluidic and SLP reagent |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110927373A (en) * | 2019-11-26 | 2020-03-27 | 北京化工大学 | Full-automatic multi-index joint detection micro-fluidic chip and device |
| CN114471762A (en) * | 2022-02-22 | 2022-05-13 | 西北大学 | Design and implementation method of micro-fluidic chip capable of controlling ultralow flow velocity |
| CN117025376A (en) * | 2023-07-06 | 2023-11-10 | 浙江工业大学 | Rapid sterile detection method based on liquid drop micro-fluidic and SLP reagent |
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